CN108098458B - 一种用于抗hpv6/11的凝胶剂 - Google Patents

一种用于抗hpv6/11的凝胶剂 Download PDF

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CN108098458B
CN108098458B CN201710673893.XA CN201710673893A CN108098458B CN 108098458 B CN108098458 B CN 108098458B CN 201710673893 A CN201710673893 A CN 201710673893A CN 108098458 B CN108098458 B CN 108098458B
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徐先顺
覃鸿恩
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Qin Hongen
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Abstract

本发明公开了一种用于抗HPV6/11的凝胶剂,其特征在于,包括江边一碗水50g，白芷20g，白鲜皮20g，卡波姆1g，丙二醇10g，三乙醇胺2g，体积浓度为95％的乙醇，纯水100g。与现有治疗药物相比，本发明治疗效果更佳，配方简单，治愈率高。

Description

一种用于抗HPV6/11的凝胶剂
技术领域
本发明涉及一种发明配方，具体为一种用于抗HPV6/11的凝胶剂，属于医疗卫生领域。
背景技术
尖锐湿疣是由人乳头瘤病毒(HPV)感染所致的以肛门生殖器部位增生性损害为主要表现的性传播疾病。HPV有不同的亚型。最常引起尖锐湿疣的HPV有6、11等。现有治疗HPV的药物疗效周期较长，治疗效果较不佳，且治疗窗窄，毒性作用较大。
发明内容
本发明的目的在于针对上述问题，克服现有技术不足，提供一种用于抗HPV6/11的凝胶剂。
为实现上述目的，本发明采用以下技术方案：
一种用于抗HPV6/11的凝胶剂,由以下质量份的原料组成：江边一碗水50g，白芷20g，白鲜皮20g，卡波姆1g，丙二醇10g，三乙醇胺2g，体积浓度为95％的乙醇，纯水100g。
进一步，上述江边一碗水、白芷和白鲜皮粗粉后经80％乙醇提取两次，第一次提取时体积浓度80％的乙醇为混合物的9倍量，第二次提取时体积浓度80％的乙醇为混合物的7倍量，提取时间为60min。两次提取液合并，将提取液依次过滤、离心、回收乙醇、干燥、粉细得到纯化后的药物。
进一步，取水50ml煮沸，放至室温，加入卡波姆使其膨胀并搅拌溶解，溶液内加入三乙醇胺调节pH至4，将药物溶于95％乙醇中，加入丙二醇混合得到混合液；所述pH为4的溶液与混合液混合，加水至100g搅拌均匀得到凝胶剂。
进一步，所述凝胶剂以每盒20g分装于软膏盒中。
本发明的有益效果为：与现有治疗药物相比，本发明治疗效果更佳，配方简单，治愈率高，毒性低，治愈率高。
具体实施方式
以下通过具体实施例进一步描述本发明，但本发明不仅仅限于以下实施例。在本发明的范围内或者在不脱离本发明的内容、精神和范围内，对本发明进行的变更、组合或替换，对于本领域的技术人员来说是显而易见的，且包含在本发明的范围之内。
一种用于抗HPV6/11的凝胶剂,由以下质量份的原料组成：江边一碗水50g,白芷20g，白鲜皮20g，卡波姆1g，丙二醇10g，三乙醇胺2g，体积浓度为95％的乙醇，纯水100g。
进一步，上述江边一碗水、白芷和白鲜皮粗粉后经80％乙醇提取两次，第一次提取时体积浓度80％的乙醇为混合物的9倍量，第二次提取时体积浓度80％的乙醇为混合物的7倍量，提取时间为60min。两次提取液合并，将提取液依次过滤、离心、回收乙醇、干燥、粉细得到纯化后的药物。
进一步，取水50ml煮沸，放至室温，加入卡波姆使其膨胀并搅拌溶解，溶液内加入三乙醇胺调节pH至4；将物溶于95％乙醇中，加入丙二醇混合得到混合液；所述pH为4的溶液与混合液混合，加水至100g搅拌均匀得到凝胶剂。
进一步，所述凝胶剂以每盒20g分装于软膏盒中。
另外，采用病毒感染细胞载体，以病毒DNA载量为变化来分析凝胶剂作用，建立抗HPV6/11引起的尖锐湿疣的鬼二白活性物质的制备工艺、质量控制与评价体系，所述步骤包括：
(1)、以HaCaT细胞为载体，取人体尖锐湿疣病理组织，获取HPV6/11病毒片段，将病毒片段置于细胞中，建立细胞模型，采用FQ-PCR技术检查其DNA载量；
(2)、将感染HPV6/11病毒的细胞植于大鼠皮下组织，建立动物模型，使用鬼二白凝胶剂局部涂抹，观察细胞组织病理情况，FQ-PCR法检测细胞HPV DNA载量。
1、疗效标准
痊愈：症状全部消失，检查正常。
有效：症状基本消失。
无效：症状无明显改善。
2、疗效结果统计表：
本表格只适用于治疗尖锐湿疣研究。
上述实施例，只是本发明的较佳实施例，并非用来限制本发明实施范围，故凡以本发明权利要求所述内容所做的等效变化，均应包括在本发明权利要求范围之内。

Claims (1)

1.一种用于抗HPV6/11的凝胶剂,其特征在于,由以下质量份的原料组成：江边一碗水50g，白芷20g，白鲜皮20g，卡波姆1g，丙二醇10g，三乙醇胺2g，体积浓度为95％的乙醇，纯水100g；上述江边一碗水、白芷和白鲜皮粗粉后经80％乙醇提取两次，第一次提取时体积浓度80％的乙醇为混合物的9倍量，第二次提取时体积浓度80％的乙醇为混合物的7倍量，提取时间为60min；两次提取液合并，将提取液依次过滤、离心、回收乙醇、干燥、粉细得到纯化后的药物，其中取水50ml煮沸，放至室温，加入卡波姆使其膨胀并搅拌溶解，溶液内加入三乙醇胺调节pH至4；将药物溶于95％乙醇中，加入丙二醇混合得到混合液；所述pH为4的溶液与混合液混合，加水至100g搅拌均匀得到凝胶剂，所述凝胶剂以每盒20g分装于软膏盒中。
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* Cited by examiner, † Cited by third party
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CN1771937A (zh) * 2005-11-04 2006-05-17 沈阳明华制药有限公司 鬼臼毒素外用膏剂及制备方法
CN1833674A (zh) * 2006-01-11 2006-09-20 北京因科瑞斯生物制品研究所 一种疣迪凝胶及其制备方法
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CN101967151A (zh) * 2010-06-28 2011-02-09 南京泽朗农业发展有限公司 一种天然产物鬼臼毒素的制备方法
CN101974008A (zh) * 2010-10-28 2011-02-16 中南大学 小八角莲中提取纯化鬼臼毒素的工艺
CN103386041A (zh) * 2013-08-10 2013-11-13 王慧丽 用于尖锐湿疣的银倍黄草膏
CN105456315A (zh) * 2015-12-06 2016-04-06 原慧玲 一种治疗扁平疣、寻常疣的药物组合物及制备

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1771937A (zh) * 2005-11-04 2006-05-17 沈阳明华制药有限公司 鬼臼毒素外用膏剂及制备方法
CN1833674A (zh) * 2006-01-11 2006-09-20 北京因科瑞斯生物制品研究所 一种疣迪凝胶及其制备方法
CN1840116A (zh) * 2006-01-24 2006-10-04 北京中科派特生物技术有限公司 治疗由人类乳头瘤病毒引起的皮肤和粘膜疾病的外用中药
CN101375845A (zh) * 2007-08-30 2009-03-04 北京天川军威医药技术开发有限公司 治疗尖锐湿疣及生殖器疱疹的鬼臼毒素外用制剂处方、系列剂型及制备方法
CN101967151A (zh) * 2010-06-28 2011-02-09 南京泽朗农业发展有限公司 一种天然产物鬼臼毒素的制备方法
CN101974008A (zh) * 2010-10-28 2011-02-16 中南大学 小八角莲中提取纯化鬼臼毒素的工艺
CN103386041A (zh) * 2013-08-10 2013-11-13 王慧丽 用于尖锐湿疣的银倍黄草膏
CN105456315A (zh) * 2015-12-06 2016-04-06 原慧玲 一种治疗扁平疣、寻常疣的药物组合物及制备

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