CN108056111A - A kind of microbial bacterial agent that wheat nutrient is promoted to absorb and preparation method thereof - Google Patents
A kind of microbial bacterial agent that wheat nutrient is promoted to absorb and preparation method thereof Download PDFInfo
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- CN108056111A CN108056111A CN201711341450.7A CN201711341450A CN108056111A CN 108056111 A CN108056111 A CN 108056111A CN 201711341450 A CN201711341450 A CN 201711341450A CN 108056111 A CN108056111 A CN 108056111A
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- Prior art keywords
- wheat
- bacterial agent
- microbial bacterial
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- microbial
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
Abstract
The present invention relates to a kind of microbial bacterial agents that wheat nutrient is promoted to absorb and preparation method thereof, belong to microbial bacterial agent research and development field.The primary raw material that the microbial bacterial agent uses is Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,15834 etc..Utilize agrobacterium rhizogenes, dodecyl sodium sulfate, urea, potassium fulvate, chitosan is configured to microbial bacterial agent by a certain percentage, applied to wheat planting, apply microbial inoculum once within every 10 days, since agrobacterium rhizogenes and wheat form a kind of symbiosis, it have stimulated the root growth of wheat and increase root absorption area, enhance the ability of wheat nutrient absorption, it has been chiefly to facilitate wheat N, the absorption of P, and then promote its growth development, greatly improve wheat quality, it agriculturally can be used for improving the quality of wheat and increasing its yield, there is huge economic benefit.
Description
Technical field
The present invention relates to a kind of microbial bacterial agents that wheat nutrient is promoted to absorb and preparation method thereof, belong to agricultural technology neck
Domain.
Background technology
Wheat (Triticum aestivum L.) is grass family wheat platymiscium, is most important cereal crops in the world
One of, it is the three big crops for being only second to rice and corn in China.Wheat is mostly extensive due to site preparation, sowing it is too shallow, root system compared with
It is weak, wheat seedling growth is made not strengthen, tillering node is in dry ground, and in the case of soil freezing, root is not hygroscopic, and overground part still has
Rising phenomenon makes wheat seeding be difficult to that internal water is maintained to balance, and be easy to cause arid death.Common Diseases have stripe rust of wheat, leaf
Rust, head blight etc..However head blight has become one of important disease for influencing yield and quality of wheat, causes decreased production,
Seriously affect Wheat Production safety and food security.It applies nitrogen and improves the generation that plant nitrogen concentration advantageously reduces head blight, increase
Add crop yield.
Microbial bacterial agent be microorganism by production expand it is numerous after, utilize viable bacteria made of the zymotic fluid of adsorbent absorption thalline
Preparation.This microbial inoculum has for seed dressing or root dipping and directly or indirectly improves soil, recover soil fertility, prevent soil-borne disease, maintain
The effects that rhizosphere microflora balance and degradation poisonous and harmful substances.The appropriate use of agricultural microbial agent can improve agricultural product
Yield improves quality of agricultural product, reduces fertilizer amount, reduce cost, improvement soil, preserve the ecological environment.
Agrobacterium rhizogenes (Agrobacterium rhizogenes) can induce the hairy root that plant generates, hairy root tool
There is the features such as growth cycle, condition of culture are simple, stability is strong, purpose product content is high.The plant that agrobacterium rhizogenes induction generates
Strain well developed root system, is conducive to the absorption of the minerals such as soil N P.Ri plasmids can induce conversion plant and generate a large amount of hair roots, promote
Into plant establishment, flourishing root system is conducive to improve plant resistance to environment stress, improves crop survival rate.So it is prepared using agrobacterium rhizogenes
Microbial inoculum is conducive to improve the nutrient absorption efficiency of wheat, further improves crop quality.
The content of the invention
It, should the object of the present invention is to provide a kind of microbial bacterial agent that wheat nutrient is promoted to absorb for above-mentioned phenomenon
Microbial bacterial agent utilizes the distinctive effect for promoting plant root growth of agrobacterium rhizogenes, is absorbed so as to increase the nutrient of wheat,
Wheat is solved in growth course since root system is undeveloped, quality is low caused by rootability is weak and the problem of low output,
The present invention provides a kind of preparation methods for the microbial bacterial agent that wheat nutrient is promoted to absorb.
The present invention is realized by following technical scheme：
A kind of microbial bacterial agent that wheat nutrient is promoted to absorb, it is characterised in that：The microbial bacterial agent is by with the following group
The raw material proportioning of part is made：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, bacterial strain derives from Chinese microorganism strain collection；
The dodecyl sodium sulfate is that the place of production of content 70% on the market is the northern village board dodecyl sodium sulfonate in Nanjing
Sodium；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
The dodecyl sodium sulfate added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
A kind of preparation process for the microbial bacterial agent that wheat nutrient is promoted to absorb is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation extremely
OD values are 0.4-0.6, must take the bacterium solution after activation, and the bacterium solution 100ml after activation is separately added into dodecyl sodium sulfate 2g, urine
Plain 40g, alginic acid 16g, chitosan 20g, mixing obtain microbial bacterial agent.
The present invention has following positive effect：
1st, the microorganism fungus kind that microbial bacterial agent of the present invention uses for agrobacterium rhizogenes strain A4, R1205, R1000,
R1601, R1022, one or more of 15834, agrobacterium rhizogenes can generate symbiosis with crop root, make its adventitious root
Growth is rapid, and induction plant generates hairy root, promotes the growth of wheat root, and by consolidating root system of plant, improving increases nutrient
Absorbability has achieved the effect that improve crop quality.
2nd, microbial bacterial agent of the present invention use agrobacterium rhizogenes bacterium solution, the components such as urea, dodecyl sodium sulfate, have into
The advantages of this is relatively low, easily obtains, and preparation method is simple, is readily put into production.
3rd, the preparation method of a kind of microbial bacterial agent that wheat nutrient is promoted to absorb using the present invention, solves wheat and exists
In growth course due to root system it is undeveloped caused by nutrient content is low and the problem of low output, there is huge economic benefit.
Description of the drawings
Fig. 1 is at different number of days (10d, 20d, 30d), does not apply microbial inoculum wheat (control group) of the present invention and applies the present invention
The nitrogen content variation diagram of microbial inoculum wheat (experimental group).
Specific embodiment
With reference to case study on implementation, the invention will be further described：
A kind of microbial bacterial agent that wheat nutrient is promoted to absorb, the microbial bacterial agent by following component raw material proportioning
It is made：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, bacterial strain derives from Chinese microorganism strain collection；
The dodecyl sodium sulfate is that the place of production of content 70% on the market is the northern village board dodecyl sodium sulfonate in Nanjing
Sodium；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
The dodecyl sodium sulfate added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
The preparation process of the microbial bacterial agent is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation extremely
OD values are 0.4-0.6, must take the bacterium solution after activation, and the bacterium solution 100ml after activation is separately added into dodecyl sodium sulfate 2g, urine
Plain 40g, alginic acid 16g, chitosan 20g, mixing obtain microbial bacterial agent.
The application process that wheat nutrient is promoted to absorb
Microbial bacterial agent is imposed on into 5 centimetres of high wheat seedlings, every plant of about 50ml diluent (extension rate：20), every 10
It applies microbial inoculum once.There is the characteristics of apparent well developed root system, nutrient absorbability enhances in wheat plant developmental process.
Application of the microbial bacterial agent in Wheat Production based on agrobacterium rhizogenes
Wheat seed 100 on the market, are divided into control group and experimental group, control group does not apply fertilizer, experimental group by every 50 one group
Fertilising, 5 centimetres of high wheat seedlings, every plant of about 50ml diluent (extension rate are imposed on by agrobacterium rhizogenes microbial inoculum：20), every 10
It applies microbial inoculum once, applies microbial inoculum altogether 3 times, observes and records Reducing sugar, the results are shown in table below for record：
The wheat plant growing state of different number of days
The result shows that the wheat for being applied with the microbial bacterial agent based on agrobacterium rhizogenes has substantially in developmental process
Survival rate it is high, the characteristics of well developed root system, nutrient absorbability is strong, and growth is vigorous, and setting percentage is high, less applies the plant phase of microbial inoculum
Than wheat quality and yield increase, result explanation, a kind of microbial bacterial agent based on agrobacterium rhizogenes of the invention
The nutrient for promoting wheat absorbs, and improves its growth quality, there is important positive effect to agricultural production.
As shown in attached drawing 1：At different number of days (10d, 20d, 30d), do not apply microbial inoculum wheat (control group) of the present invention and apply
The nitrogen content of microbial inoculum wheat (experimental group) of the present invention is added to change.
As shown in Table 1：At different number of days (10d, 20d, 30d), do not apply microbial inoculum wheat (control group) and application microbial inoculum is small
Total root long, average root diameter, root surface area, root volume and the variation of tip of a root number of wheat (experimental group).
Table 1 applies influence of the microbial inoculum to growth of wheat roots
Table 1：At different number of days (10d, 20d, 30d), do not apply microbial inoculum wheat (control group) and apply the (experiment of microbial inoculum wheat
Group) total root long, average root diameter, root surface area, root volume and the tip of a root number variation.
By kjeldahl apparatus and root scanner etc., the wheat plant for measuring application microbial inoculum less applies the wheat plant of microbial inoculum
The variation of nitrogen content and the variation of root growth in the nutrient content of strain.The data obtained further illustrates, is applied with the small of microbial inoculum
Wheat has stronger nutrient absorbability in developmental process, and rootability is strong, eugonic feature, the plant less applied fertilizer
It compares, the quality of wheat increases.A kind of microbial bacterial agent based on agrobacterium rhizogenes of the invention promotes supporting for wheat
Part absorbs, and improves the growth quality of wheat, there is important positive effect to agricultural production.
Claims (4)
1. a kind of microbial bacterial agent that wheat nutrient is promoted to absorb, which is characterized in that the microbial bacterial agent is by following component
The raw material of proportioning is made：
Strain contained by the agrobacterium rhizogenes liquid for common Agrobacterium rhizogenesA4, R1205, R1000, R1601, R1022,
One or more of 15834, strain derives from Chinese microorganism strain collection；
The dodecyl sodium sulfate is that the place of production of content 70% on the market is the northern village board dodecyl sodium sulfate in Nanjing；
The urea that it is on the market 99.9% by the content of Beijing Kang Puhui dimensions Science and Technology Ltd. production that the urea, which is,；
The potassium fulvate is the potassium fulvate of content >=70% by the production of Jinan Rong Guan Co., Ltds on the market；
The chitosan is that the active principle content produced on the market by hundred taste bio tech ltd of Hebei is
99.8% chitosan.
2. a kind of preparation method of microbial bacterial agent that wheat nutrient is promoted to absorb according to claim 1, feature exist
In：The agrobacterium rhizogenes bacterium solution is the Agrobacterium bacterium solution that OD values are 0.4-0.6.
3. a kind of preparation method of microbial bacterial agent that wheat nutrient is promoted to absorb according to claim 1, feature exist
In：The dodecyl sodium sulfate added in microbial inoculum is surfactant, can promote absorption of the plant root to microbial inoculum.
4. a kind of preparation method of microbial bacterial agent that wheat nutrient is promoted to absorb according to claim 1, feature exist
In：Its preparation process is：
By agrobacterium rhizogenes strain A4, R1205, R1000, R1601, R1022, one or more of 15834 activation to OD values
For 0.4-0.6, the bacterium solution after activation must be taken, the bacterium solution 100ml after activation is separately added into dodecyl sodium sulfate 2g, urea
40g, alginic acid 16g, chitosan 20g, mixing obtain microbial bacterial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711341450.7A CN108056111A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial bacterial agent that wheat nutrient is promoted to absorb and preparation method thereof |
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| CN201711341450.7A CN108056111A (en) | 2017-12-14 | 2017-12-14 | A kind of microbial bacterial agent that wheat nutrient is promoted to absorb and preparation method thereof |
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