CN108610182A - A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function - Google Patents

A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function Download PDF

Info

Publication number
CN108610182A
CN108610182A CN201810749558.8A CN201810749558A CN108610182A CN 108610182 A CN108610182 A CN 108610182A CN 201810749558 A CN201810749558 A CN 201810749558A CN 108610182 A CN108610182 A CN 108610182A
Authority
CN
China
Prior art keywords
fertilizer
compound fertilizer
function
prophyiaxis
bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810749558.8A
Other languages
Chinese (zh)
Inventor
王斌
张琪
盘文政
赵学通
何月秋
尚海丽
赵崇钧
杨丽娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fermentation Research Center Yunnan Co Ltd
Original Assignee
Fermentation Research Center Yunnan Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fermentation Research Center Yunnan Co Ltd filed Critical Fermentation Research Center Yunnan Co Ltd
Priority to CN201810749558.8A priority Critical patent/CN108610182A/en
Publication of CN108610182A publication Critical patent/CN108610182A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/30—Layered or coated, e.g. dust-preventing coatings
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention discloses a kind of compound fertilizer with prophyiaxis and promoting growth function, it includes composite fertilizer and is wrapped in the function bacterium powder on composite fertilizer surface；Preparation method includes the following steps：Function bacteria strain progress aerobic fermentation is obtained zymotic fluid, zymotic fluid is spray-dried, function bacterium bacterium powder is obtained by step 1；Step 2, the preparation of the raw materials for production of compound fertilizer, the compound fertilizer production technology carry out the granular composite fertilizer of fertilizer production using rotary drum prilling process；Step 3, by the function bacterium bacterium powder of step 1 dust after step 2 granulation of compound fertilizer coating this during mix with compound fertilizer, so that function bacterium bacterium powder is wrapped in compound fertilizer granule surface, it is composite coated that finished product is made；Step 4, by after the finished product packing of step 3 to get to the compound fertilizer product with prophyiaxis and promoting growth function.Active microorganism is combined with compound fertilizer, reaches improvement soil physical and chemical property, reduces fertilizer demand amount, improves utilization rate of fertilizer, fertilising cost is saved, promotes the purpose of plant growth.

Description

A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function
Technical field
The present invention relates to fertilizer field more particularly to a kind of compound fertilizers and its preparation side with prophyiaxis and promoting growth function Method.
Background technology
The application of chemical fertilizer improves the yield and production efficiency of crops.In recent decades, the extensive use of chemical fertilizer Soil physico-chemical property deterioration and problem of environmental pollution is caused increasingly to cause the concern of people.Although chemical fertilizer is China agricultural obtain at Significant contributor just, it plays great function for improving the grain yield of unit area, but China's agricultural does not conform to for a long time Reason the problem of being brought using chemical fertilizer, becomes increasingly conspicuous, including soil nutrient is unbalance, hardened, acidification, salination, environmental pollution etc..Fertilizer Expect that amount of application is high, plant recovery of nutrient is relatively low, and it has been a universal common recognition to have negative effect to agricultural product quality and environment.To protect Demonstrate,prove the lasting development of agricultural production health, research and develop environment-friendly high-efficiency new-type fertilizer become our fertilizer production enterprises focus and Impetus.
Relatively more are composite fertilizers now, and compound fertilizer refers to the change containing two or more nutrient Fertilizer, composite fertilizer have many advantages, such as that nutrient content is high, accessory ingredient is few and physical behavior is good, for balance fertilizing, improve fertilizer utilization Rate promotes the stable high yield of crop to have highly important effect.But there are also disadvantages for it, for example its nutrients ratio is always It is fixed, and nutrient type, quantity and the ratio needed for different soils, Different Crop are various.
Invention content
The purpose of the present invention is to solve the shortcomings of the prior art and providing the compound fertilizer with prophyiaxis and promoting growth function And preparation method thereof.Active microorganism is combined with compound fertilizer, while carrying out crop fertilization, utilizes beneficial microbe Activation improvement is carried out to soil, reaches improvement soil physical and chemical property, reduces fertilizer demand amount, improves utilization rate of fertilizer, saving is applied Fertile cost promotes the purpose of plant growth, that is, can guarantee the quick-acting of fertilizer, and can carry out improvement reparation to soil environment, be Carry out the new selection of soil improvement and soil remediation in agricultural production.
In order to achieve the above objectives, the present invention is achieved through the following technical solutions.
A kind of compound fertilizer with prophyiaxis and promoting growth function, it includes composite fertilizer and is wrapped in the function on composite fertilizer surface Bacterium powder, the ratio that the function bacterium powder is added are that the weight fraction of total amount is 0.2%~0.4%；
The function bacterium is a bacillus amyloliquefaciens, and preservation registration number is CGMCC No.15851.
A kind of preparation method of the compound fertilizer with prophyiaxis and promoting growth function, it includes the following steps：
Function bacteria strain progress aerobic fermentation is obtained zymotic fluid, zymotic fluid is spray-dried, work(is obtained by step 1 It can bacterium bacterium powder；
Step 2, the preparation of the raw materials for production of compound fertilizer, the compound fertilizer production technology use rotary drum prilling process The granular composite fertilizer of fertilizer production is carried out, the compound fertilizer production technology complies with standard GB/T 15063-2009；
Step 3, by the function bacterium bacterium powder of step 1 dust after step 2 granulation of compound fertilizer coating this during with Compound fertilizer mixes, and function bacterium bacterium powder is made to be wrapped in compound fertilizer granule surface, composite coated that finished product is made；
Step 4, by after the finished product packing of step 3 to get to the compound fertilizer product with prophyiaxis and promoting growth function.
Further, the culture medium prescription of the function bacteria strain progress aerobic fermentation is as follows in parts by weight：Analysis for soybean powder 10~16 parts, 7~13 parts of sucrose, 1~5 part of peptone, 2~8 parts of corn flour, 3~9 parts of calcium carbonate, 3~7 parts of fish meal, calcium chloride 0.2~0.8 part, 0.2~0.7 part of potassium dihydrogen phosphate, 0.2~0.8 part of dipotassium hydrogen phosphate, 0.1~0.5 part of manganese sulfate, hydroxide 0.1~0.5 part of sodium, 2~7 parts of sodium chloride, 1~2 part of antifoaming agent.Antifoaming agent is commercial product, such as Yantai Heng Xin chemical industry THIX-298 type products.
Further, the inoculum concentration (weight percent) that the function bacteria strain carries out aerobic fermentation is 0.5%~ 1.5%.
Further, the temperature that the function bacteria strain carries out aerobic fermentation is 35 DEG C~39 DEG C；Incubation time is 1~3 It.
Further, the living bacteria count of the function bacterium bacterium powder is more than 3.0 × 1010cfu/g。
A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function of the present invention.By active microorganism and again Hefei material is combined, and while carrying out crop fertilization, activation improvement is carried out to soil using beneficial microbe, reaches improvement soil Earth physicochemical character reduces fertilizer demand amount, improves utilization rate of fertilizer, saves fertilising cost, promotes the purpose of plant growth, can Ensure the quick-acting of fertilizer, and improvement reparation can be carried out to soil environment, is to carry out soil improvement in agricultural production to repair with soil Multiple new selection.
Specific implementation mode
With reference to embodiment, the present invention will be further described, but is not limited to the content on specification.
The technical scheme is that：
(1) prepared by function strain：WSWFJ-11 bacterial strains are subjected to aerobic fermentation, zymotic fluid is subjected to drying of dusting, is obtained WSWFJ-11 bacterium powders, living bacteria count are controlled 3.0 × 1010Cfu/g or more.
(2) preparation of raw materials for production：The preparation of compound fertilizer raw materials for production, since this patent production technology is suitable for highly concentrated All compound fertilizers of degree, middle concentration and low concentration conventional formulation, so to composition of raw materials without Special controlling and index request, it is raw Production. art carries out fertilizer production using rotary drum prilling process.Compound fertilizer production technology complies with standard GB/T 15063-2009.
(3) addition of function strain：By WSWFJ-11 bacterium powders dust after granulation of fertilizer coating this during with it is compound Fertilizer mixes, and WSWFJ-11 bacterium powders is made to be wrapped in compound fertilizer granule surface, composite coated that a kind of new exclusive fertilizer has been made Material, the adding proportion of bacterium powder are the 0.2%~0.4% of total weight of material number.
(4) to get to the compound fertilizer finished product with prophyiaxis and promoting growth function after product packaging.
The function strain WSWFJ-11 is screened by Fermentation Research Center Yunnan Co., Ltd. It arrives, is a bacillus amyloliquefaciens, preservation registration number is CGMCC No.15851
Illustrate with reference to specific embodiment.
Specific embodiment 1：Influence of the micro organism composite fertilizer to corn growth.
1.1 material
For planting experimentally son：Luo Dan 6 is produced by Yunnan great Tian Zhong industry Co., Ltd.WSWFJ-11 bacterial strains：It is big by Crop Diseases in Yunnan It learns and screens and provide, effective viable bacteria amount >=3.0 × 10 of bacterium powder10cfu.g-1.Base fertilizer：KNO3(N >=13.5%, K2O≥ 44.5%), 15-15-15 (N >=15%, P2O5>=15%, K2O >=15%) it is carried by Yunnan Yunye Fertilizer Co., Ltd. For.
The compounding of 1.2 functional microorganism bacterium powders and composite fertilizer
It is granulated (table 1) using WSWFJ-11 bacterium powders and composite fertilizer's compounding, composite fertilizer is produced using rotary drum prilling process, function Bacterium powder is wrapped in fertilizer surface, and coating in fertilizer granulating drying stage in the form of applying powder, and bacterium powder is respectively to account for the total matter of fertilizer 0.4%, 0.3%, 0.2%, 0.1% dosage of amount is added in base fertilizer, is respectively set to formula 1,2,3,4, hereinafter referred to as Micro organism composite fertilizer, base fertilizer is as a contrast (CK).
Table 1 is for trying fertilizer fundamental technology parameter
The influence that 1.3 micro organism composite fertilizers grow Maize Seedling
Using 1.2 different formulations compound fertilizer potting corns, potting soil and different formulations fertilizer mixing are sub-packed in nutrition Basin (diameter 13cm × high 12cm), per 4, basin seeding corn seed, 5 processing of two kinds of basis fertilizer, each setting 3 is different to be used Amount is 2g, 1.6g, 1.2g, i.e., each 15 processing of basis fertilizer are repeated 4 times, routinely water management, wait for that corn seedling is unearthed 3 weeks Afterwards, 12 plants of maize seedling, the morphological indexs such as investigation corn type height, root long, overground part, underground part fresh weight are chosen in each processing.And it answers Data statistics is completed with EXCEL 2007 and tabulation, SPSS 20 carry out variance analysis and Duncan Multiple range tests, wherein confidence level It is 95%, filters out each fertilizer most side of adaptation.
2 results and analysis
2.1 KNO3The influence that micro organism composite fertilizer grows Maize Seedling
After compound potassium nitrate fertilizer and bacterium powder compounding are granulated, when amount of application 2g, 1.6g and 1.2g, each microbial manure is handled Corn type height is superior to control treatment (table 2), shows as formula 1>Formula 2>Formula 3>Formula 4>CK, and the root long compareed is then excellent In the processing of each formula fertilizer, overall performance CK>Formula 1>Formula 2>Formula 3>Formula 4.Generally speaking, before the rational application of fertilizer It puts, for such micro organism composite fertilizer compared with CK, the growth promotion advantage for being formulated 1,2 pair of Maize Seedling aerial part is notable, per basin The 2g processing of base fertilizer is above using the corn type height of 1.6g and 1.2g, that is, synergistic effect of losing weight is apparent.As function is micro- The metabolic activity of the reduction of biological additive amount, the trend that the type height and root long of Maize Seedling taper off, functions microorganism promotees Into the growth of corn overground part.The root long of CK processing is better than the processing of each formula, it may be possible to because microbiota metabolic activity generates Growth regulatory substance caused by.With only with the CK of basis fertilizer compared with, when fertilizer amount 2.0g, 1,2,3,4 type height of formula increase respectively Add 5.76%, 3.49%, 2.42%, 1.82%, root long reduces 6.09%, 6.20%, 8.09%, 10.71% respectively；1.6g When, type height increases separately 17.48%, 13.81%, 6.59%, 5.86%, root long reduces 15.34% respectively, 20.25%, 24.90%, 23.54%；When 1.2g, type height increases separately 11.15%, 7.61%, 1.22%, 0.29%, and root long is reduced respectively 16.81%, 22.49%, 25.03%, 23.40%.
2 KNO of table3Influence of the micro organism composite fertilizer to Maize Seedling type height and root long
Note：Means standard deviation is marked with value expression significant difference in P=0.05 levels of lowercase in same row (Duncan methods), uppercase value indicate significant difference (the Duncan methods) in P=0.01 levels.
Each formula fertilizer processing overground part fresh weight and underground part fresh weight are all higher than control treatment (table 3).The root of control treatment Elongated thin and weak, the root of formula fertilizer processing relatively compares short but sturdy, illustrates the need that WSWFJ-11 promotes maize root system to nutrient It asks.With the reduction of WSWFJ-11 bacterium powder contents, trend that the overground part fresh weight and underground part fresh weight of Maize Seedling also taper off. Between same formula, when expecting dosage 1.6g, corn aerial part growing way is better than 2.0g and 1.2g, and is formulated 1 and is significantly higher than at control Reason, this may be just related with the nutrient requirement characteristics coupling of corn with the metabolic activity of microorganism, fertilizer amount.With CK ratios When compared with, fertilizer amount 2.0g, 1,2,3,4 processing corn overground part fresh weights of formula increase separately 50.93%, 21.43%, 20.81%, 8.07%, underground part fresh weight increases separately 67.66%, 42.51%, 24.55%, 22.75%；When 1.6g, formula 1,2,3,4 overground part fresh weights increase separately 38.61%, 36.11%, 31.94%, 18.06%, and underground part fresh weight increases separately 33.49%, 6.05%, 9.00%, 5.58%；When 1.2g, overground part fresh weight compares no significant difference, underground part fresh weight with mark fertilizer Only formula 1 is significantly higher than outside control, other 3 are formulated and compare no significant difference.
3 KNO of table3Influence of the micro organism composite fertilizer to Maize Seedling overground part fresh weight and underground part fresh weight
The influence that 2.2 15-15-15 micro organism composite fertilizers grow Maize Seedling
Under the conditions of identical rate of fertilizer, 15-15-15 micro organism composite fertilizers are compared with base fertilizer, and corn is in plant height With equally show certain advantage (table 4) in root long, when fertilizer amount 2.0g, 1,2,3,4 type height of formula increase separately 5.10%, 8.47%, 6.16%, 4.11%, root long increases separately 15.09%, 16.37%, 5.82%, 0.64%；When 1.6g, Type height increases separately 3.89%, 4.97%, 0.67%, 0.45%, root long increases separately 5.67%, 6.29%, 9.75%, 11.00%；When 1.2g, type height increases separately 6.52%, 7.72%, 7.02%, 5.28%, root long reduces 40.96% respectively, 49.06%, 36.78%, 34.54%.
Influence of the 4 15-15-15 micro organism composite fertilizers of table to Maize Seedling type height and root long
Similarly, compared with CK, when fertilizer amount 2.0g, 1,2,3,4 overground part fresh weights of formula increase separately 69.86%, 63.01%, 71.23%, 53.88%, underground part fresh weight increases separately 20.44%, 18.25%, 5.84%, 15.33%；1.6g When, 1,2,3,4 overground part fresh weights of formula increase separately 13.60%, 6.65%, 4.23%, 3.02%, and underground part fresh weight increases respectively Add 11.97%, 9.15%, 7.04%, 7.04%；When 1.2g, 1,2,3,4 overground part fresh weights of formula increase separately 18.77%, 20.31%, 16.0%, 12.92%, underground part fresh weight increases separately 30.4%, 35.2%, 28.8%, 23.2% (table 5).
Influence of the 5 15-15-15 micro organism composite fertilizers of table to Maize Seedling overground part fresh weight and underground part fresh weight
Specific embodiment 2：Micro organism composite fertilizer shelf life measures.
1, fertilizer items technology and Indexs measure
Living bacteria count detects under the conditions of 1.1 different moisture contents
The most adaptation side obtained in embodiment 1 sealing is sub-packed in sterilizing tissue culture bottle and is stored in shady and cool dry place and is used as pair According to (CK), while it is the processing under the conditions of 10%, 20%, 30%, 40% (deliquescing completely), periodic detection WSWFJ- that humidity, which is arranged, 11 effective viable bacteria amounts, and record statistics.
Living bacteria count detects under 1.2 different soils Water Content Conditions
Most suitable formula fertilizer is pressed into fertilizer with sterilizing (interference for excluding other microorganisms) red soil for determining moisture content in advance Native ratio 1:Be fitted into after 100 mixings in sterilizing tissue culture bottle and sterile water be added, make soil moisture be respectively 10%, 20%, 30%, 40%, 50%, 60%, and setting does not add the processing of sterile water as a contrast (CK), is sealed and stored in the tissue culture bottle that sterilizes, periodically Detection function microorganism colonizes situation in red soil, and record counts effective viable bacteria amount.
Function bacterium quantity detects in 1.3 fertilizer storage processes
Be coated with counting method measurement function bacterium number amount using plating dilutions, weigh fertilizer 5g (deduct water content) be dissolved in 45mL without In bacterium water, oscillation 30min ensures to carry out gradient dilution after sample fully dissolves, and dilution is coated on LB culture medium flat plates, 37 DEG C constant temperature incubation 36h or so writing function bacterium number amount.
Microorganism shelf life in 1.4 micro organism composite fertilizers
In micro organism composite fertilizer, the survival ability of function bacterium plays conclusive effect to the quality and fertilizer efficiency of fertilizer.Choosing Take KNO3The fertilizer of micro organism composite fertilizer bacterium powder additive amount 0.4% and 15-15-15 composite fertilizers bacterium powder additive amount 0.2% is had Living stems are imitated, effective viable bacteria theoretical value is respectively 6 × 108Cfu/g and 3.0 × 108cfu/g.After room temperature storage 6 months, Either KNO3Or the WSWFJ-11 in composite fertilizer 15-15-15 can preferably survive (table 6).When water content is more than 20% When, function bacterium living bacteria count is in first to increase the trend dropped afterwards with the extension of period of storage.After storage 2,4,6 months, KNO3In have Viable detection is imitated respectively to divide up to effectively Viable detection in 82.17%, 57.5%, 36.67% or more, 15-15-15 composite fertilizers Not up to 68.67%, 62.67%, 37.33% or more.Different moisture content store the result shows that：WSWFJ-11 bacterium powders with gemma After base fertilizer is added, bacterial strain survival rate is still higher, can maintain 1.0 × 108Cfu/g or more (national standard 2 × 107Cfu/g), the process conditions and produce reality demand of such micro organism composite fertilizer can be fully met.
6 different moisture content of table is to KNO3With the influence of effective viable bacteria amount in 15-15-15 micro organism composite fertilizers
Micro organism composite fertilizer is by loam than 1：After 100 mixings, contain KNO3In soil living bacteria count theory radix be 6.0 × 106Living bacteria count theory radix in cfu/g, 15-15-15 containing composite fertilizer soil is 3.0 × 106The two is placed in by cfu/g The result (table 7) of living bacteria count detection under the conditions of different soil moisture shows：Containing KNO3Soil conventional treatment, 10% soil Decline 76.83% and 54.17% respectively compared with effective viable bacteria theory radix, living bacteria count in soil after being stored 6 months under humidity, And in 20%, 30%, 40%, 50%, 60% soil moisture living bacteria count increase separately 8.33%, 22.17%, 31.33%, 6.17%, 15.33%；Similarly, after being stored 6 months under the soil of 15-15-15 containing composite fertilizer conventional treatment, 10%, 20% humidity Decline 60.00%, 45.00%, 12.67% respectively compared with effective viable bacteria theory radix, living bacteria count in soil；30%, 40%, 50%, living bacteria count increases separately 52.67%, 9.33%, 15.33%, 43.33% under 60% soil moisture.Result above Show：After the incorporation of fertilizerin the soil, under certain humidity conditions, functional microorganism can successfully be colonized in soil and be bred, can Can be sterilized soil with soil, dead after most indigenous microorganisms are sterilized, functional microorganism is related as dominant microflora, so And whether can also reach this effect in natural soils about the fertilizer, it also needs further to study.
7 different soil moisture of table is to KNO3With the influence of effective viable bacteria amount in 15-15-15 micro organism composite fertilizers
2, conclusion
WSWFJ-11 is the bacterial strain for having fine Soluble phosphorus potassium decomposing and fixed nitrogen function, by WSWFJ-11 bacterium powders with 0.2%~ 0.4% dosage is added to surface package in potassium nitrate and 15-15-15 composite fertilizers has the function of good fertilizer efficiency as after granulated fertilizer. The addition of WSWFJ-11, can significantly reduce the dosage of chemical fertilizer, and under same formulation condition, potassium nitrate micro organism composite fertilizer is applied With 1.6g compared with 2.0g and 1.2g processing, corn type is high and ground, underground fresh weight are optimal, and root long is not as good as mark fertilizer processing, it may be possible to Due to WSWFJ-11 generate secondary metabolite have the function of certain fertilizer efficiency, cause root system become fertilizer weaken.15-15-15 is micro- Biological compound fertilizer applies 1.2g compared with 2.0g and 1.6g, and plant above ground portion, underground part growing way are optimal, and the growth formula processing of root is equal It is opposite with the result of potassium nitrate better than mark fertilizer control, it may be possible to since phosphorus nourishing supply is abundant, to further promote root system and stretch Length is related.Generally speaking, WSWFJ-11 and chemical fertilizer compound can effectively facilitate plant strain growth, increase biomass, reduce Chemical fertilizer amount of application.
This research makes micro organism composite fertilizer in 10% to 40% range of water content using WSWFJ-11 bacterial strains, effectively living Bacterium number is during storing half a year in downward trend after first increasing, after 6 months, the potassium nitrate and 15-15- of water content 10% Living bacteria count can also be respectively up to 2.20 × 10 in 15 composite fertilizers8Cfu/g and 1.12 × 108Cfu/g or more, survival rate reach respectively 36.67% and 37.33% or more, overall quality meets existing national composite microbiological fertilizer standard.
Specific embodiment 3：Influence of the prophyiaxis and promoting growth micro organism composite fertilizer to tobacco leaf production.
1. purpose
From the reality of local tobacco leaf production, by compound fertilizer in the form of microbial bacterial agent is combined, in conjunction with flue-cured tobacco Basic condition and Fertilization Situation are planted, carries out adjustment and the correlation test of tobacco fertilization structure, to pass through fertilizer application mode, fertilizer The change of material formula, fertilizer properties improves tobacco plant nutrition supply from fertilising means, improves the fertilization structure of tobacco, reach cigarette Careless early growth and quick tiller early period, later stage fall Huang in due course, improve tobacco-growing soil physicochemical character, ensure leaf tobacco production while upgrading synergy Keep the target of sustainable development.
2. materials and methods
2.1 test material
2.1.1 test site
Test site be nine villages and small towns of Yuxi Dengjiang County dry grind the big graveyard in stone village, totally two mu.Soil is loam, soil fertility rank In, it in irrigation conditions, is irrigated using water cellar water, topsoil thickness is 20-30cm.It is preceding to be used as pea, applied urea.Because experimental field The mode managed moisture in the soil dig reservoir, therefore the pool is taken to apply will first test fertilizer according to processing, be applied respectively experimental field under the help of peasant household On, tobacco seedlings transplanting, watering are carried out later, and seeding row spacing is 50cm × 120cm.
2.1.2 material to be tested
This experiment tobacco seedlings kind is cloud and mist 97, and seedling age is about 3 half a months, using nutritional bag, non-leaf-cutting.
2.1.3 for trying fertilizer
" cloud leaf board " 15-5-25 Special compound fertilizer for tobacco of Yunnan Yunye Fertilizer Co., Ltd.'s production, disease prevention growth-promoting Long functional biological microbial inoculum.
2.2 experimental design
At the different settings 3 for the function microbial inoculum additive amount that base manure (base fertilizer) part is added in the fertilising of this experimental evidence Reason, 3 repetitions, using completely random district's groups.
Handle 1 (ck)：It is local conventional；
Processing 2：+ 0.2% functional biological microbial inoculum of 15-5-25 Special compound fertilizer for tobacco；
Processing 3：+ 0.4% functional biological microbial inoculum of 15-5-25 Special compound fertilizer for tobacco；
Each processing is in addition to base fertilizer type is different with proportioning, and other production measures are consistent, according to local High Quality Tobacco production technology It is managed.
2.3 field management
2.3.1 nursery and transplanting
Using nutritional bag, non-leaf-cutting, May 18, basal dressing was transplanted simultaneously.
2.3.2 cultivation management measure
Local conventional fertilizer application base manure is 40kg/ mus of composite fertilizer (16-6-20), and test process is then 43kg/ mus, and the two applies nitrogen Amount should be consistent.
Growth has some aphids early period, and diluting 1,000 times using dimethoate pesticide is sprayed, and it is climacteric that part occurs in the later stage Spot, on adopting roasting influence less.
Top dressing on June 22 is primary, and top dressing is to be formulated the special top dressing of tobacco of 10-0-30, and 20kg/ mus pour applying.
July 26 bound.
2.4 investigation records
2.4.1 breeding time is investigated：
Investigate tobacco seedlings transplanting time, group's phase, prosperous long-term, squaring period and the time for adopting the roasting phase of different disposal
2.4.2 economical character is investigated：
In group's phase, prosperous long-term, squaring period, adopts roasting the previous day and choose that 5 plants of almost the same cigarettes of growing way measure plant height, diameter encloses And the leaf of middle part maximum leaf is long, leaf width.
2.4.3 yield and quality is investigated：
According to cigarette strain mature condition, abundant maturation is adopted roasting, investigates yield, the output value, average price and the better-than-average cigarette of different disposal Ratio, the effect that comparative analysis is respectively handled.
2.4.4 tobacco leaf intrinsic chemical constituent analysis
It adopts roasting processing selection each 1kg of C3F and B2F each after the completion and unifies inspection, carry out tobacco leaf intrinsic chemical constituent analysis.
3. analysis and discussion
Influence of 3.1 micro organism composite fertilizers to tobacco breeding time：
Table 8 breeding time investigation records table
Processing Transplanting time Group's phase It is prosperous long-term Squaring period Adopt the roasting phase
Processing one May 19 June 12 June 30 July 23 August 13 days
Processing two May 19 June 12 June 30 July 23 August 13 days
Processing three May 19 June 11 June 29 July 21 August 13 days
As can be seen from the above data, the relatively processing one of the breeding time of processing three and processing two are shortened, processing one and place Manage second indifference, it was demonstrated that the amount of application for improving functional microorganism can reach tobacco early growth and quick tiller early period, the later stage falls yellow in due course Effect.
Influence of 3.2 micro organism composite fertilizers to tobacco economical character：
30 days economical character investigation records tables after table 9 is transplanted
45 days economical character investigation records tables after table 10 is transplanted
60 days economical character investigation records tables after table 11 is transplanted
75 days economical character investigation records tables after table 12 is transplanted
90 days economical character investigation records tables after table 13 is transplanted
120 days economical character investigation records tables after table 14 is transplanted
Data above investigation is 5 plants of average value, is shown according to data above, and experimental plot flue-cured tobacco breeding time meets High Quality Tobacco life Long rule, crop field are grown fine, and cigarette strain well developed root system, stalwartness, resistance are strong.Growth early period, each processing difference was little；In growth Phase processing one has more significant advantage in terms of plant height, stem girth, but three leaf areas of processing are larger, and processing two is differed with processing three Less；No matter Later growth processing three has been above in plant height, stem girth etc. handles one, and processing two differs not with processing three Greatly, the two differs larger with processing one.Whole to compare, three economical characters of processing are best.
It follows that three processing growing way othernesses early period are little, but occur lacking of staying power using composite fertilizer merely Phenomenon, and the functional microorganism in micro organism composite fertilizer can decompose the insoluble nutrient in soil, therefore handle for two, three later stages of processing Growing way is preferable, and leaf area is larger, and the yield of tobacco leaf can also increase.
Influence of 3.3 micro organism composite fertilizers to tobacco economic character：
15 economic characters investigation records table of table
No matter it is all highest that data above, which can be seen that processing three in the output value or average price, first it is minimum to handle, Show that micro organism composite fertilizer can effectively improve the output value, yield compared with Common compound fertilizer, while also having one to the quality of tobacco leaf Fixed raising also has certain effect to improving upper medium grade cigarette ratio.
Influence of 3.4 micro organism composite fertilizers to tobacco leaf intrinsic chemical ingredient：
16 C of table3F grade tobacco leaf intrinsic chemical constituent analysis record sheets
17 B of table2F grade tobacco leaf intrinsic chemical constituent analysis record sheets
For the tobacco leaf intrinsic chemical component content respectively handled above in addition to chlorinity is relatively low, remaining meets sound tobacco mark It is accurate.Three nitrogen pools and general potassium amount highest are handled for comparing, later stage nutrient is more sufficient, and chlorinity is higher, total reducing sugar and reduced sugar Content it is also higher, this has preferable influence to the mouthfeel of tobacco leaf, and nicotine content is relatively low, and toxicity is smaller.Comparatively one is handled Later stage fertilizer efficiency it is relatively low, quality of tobacco is also slightly worse, is more toxic.Handle two nicotine contents and processing be one close, other qualities with It is close to handle three.This shows that micro organism composite fertilizer has a great impact to improving quality of tobacco, especially functional microorganism microbial inoculum There is prodigious positive effect to reducing nicotine content of tobacco leaves.
4 summarize
4.1 micro organism composite fertilizers fertilizer efficiency compared with Common compound fertilizer is longer, while economic character and cigarette to improving tobacco Leaf intrinsic chemical quality has a certain impact；
For 4.2 prophyiaxis and promoting growth function microbial inoculums to increasing compound fertilizer fertilizer efficiency, improving the tobacco production output value has prodigious effect；
4.2 prophyiaxis and promoting growth function microbial inoculums can make the reduction of blade nicotine content, and total reducing sugar and content of reducing sugar rise, to carrying High quality of tobacco has certain positive effect.
A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function of the present invention.By active microorganism and again Hefei material is combined, and while carrying out crop fertilization, activation improvement is carried out to soil using beneficial microbe, reaches improvement soil Earth physicochemical character reduces fertilizer demand amount, improves utilization rate of fertilizer, saves fertilising cost, promotes the purpose of plant growth, can Ensure the quick-acting of fertilizer, and improvement reparation can be carried out to soil environment, is to carry out soil improvement in agricultural production to repair with soil Multiple new selection.
Obviously, the above embodiment of the invention be only to clearly illustrate example of the present invention, and not be Restriction to embodiments of the present invention.For those of ordinary skill in the art, on the basis of the above description also It can make other variations or changes in different ways.Here all embodiments can not be exhaustive.It is every to belong to this Row of the obvious changes or variations that the technical solution of invention is extended out still in protection scope of the present invention.

Claims (6)

1. a kind of compound fertilizer with prophyiaxis and promoting growth function, it is characterised in that：It includes composite fertilizer and is wrapped in composite fertilizer The function bacterium powder on surface, the ratio that the function bacterium powder is added are that the weight fraction of total amount is 0.2%~0.4%；
The function bacterium is bacillus amyloliquefaciens, and preservation registration number is CGMCC No.15851.
2. a kind of preparation method of compound fertilizer with prophyiaxis and promoting growth function according to claim 1, feature exist In：It includes the following steps：
Function bacteria strain progress aerobic fermentation is obtained zymotic fluid, zymotic fluid is spray-dried, function bacterium is obtained by step 1 Bacterium powder；
Step 2, the preparation of the raw materials for production of compound fertilizer, the compound fertilizer production technology are carried out using rotary drum prilling process The granular composite fertilizer of fertilizer production, the compound fertilizer production technology comply with standard GB/T 15063-2009；
Step 3, by the function bacterium bacterium powder of step 1 dust after step 2 granulation of compound fertilizer coating this during with it is compound Fertilizer mixes, and function bacterium bacterium powder is made to be wrapped in compound fertilizer granule surface, composite coated that finished product is made；
Step 4, by after the finished product packing of step 3 to get to the compound fertilizer product with prophyiaxis and promoting growth function.
3. a kind of preparation method of compound fertilizer with prophyiaxis and promoting growth function according to claim 2, feature exist In：The culture medium prescription that the function bacteria strain carries out aerobic fermentation is as follows in parts by weight：10~16 parts of analysis for soybean powder, sucrose 7 ~13 parts, 1~5 part of peptone, 2~8 parts of corn flour, 3~9 parts of calcium carbonate, 3~7 parts of fish meal, 0.2~0.8 part of calcium chloride, phosphorus 0.2~0.7 part of acid dihydride potassium, 0.2~0.8 part of dipotassium hydrogen phosphate, 0.1~0.5 part of manganese sulfate, 0.1~0.5 part of sodium hydroxide, 2~7 parts of sodium chloride, 1~2 part of antifoaming agent.
4. a kind of preparation method of compound fertilizer with prophyiaxis and promoting growth function according to claim 3, feature exist In：The inoculum concentration (weight percent) that the function bacteria strain carries out aerobic fermentation is 0.5%~1.5%.
5. a kind of preparation method of compound fertilizer with prophyiaxis and promoting growth function according to claim 2, feature exist In：The temperature that the function bacteria strain carries out aerobic fermentation is 35 DEG C~39 DEG C；Incubation time is 1~3 day.
6. a kind of preparation method of compound fertilizer with prophyiaxis and promoting growth function according to claim 2, feature exist In：The living bacteria count of the function bacterium bacterium powder is more than 3.0 × 1010cfu/g。
CN201810749558.8A 2018-07-10 2018-07-10 A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function Pending CN108610182A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810749558.8A CN108610182A (en) 2018-07-10 2018-07-10 A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810749558.8A CN108610182A (en) 2018-07-10 2018-07-10 A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function

Publications (1)

Publication Number Publication Date
CN108610182A true CN108610182A (en) 2018-10-02

Family

ID=63666329

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810749558.8A Pending CN108610182A (en) 2018-07-10 2018-07-10 A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function

Country Status (1)

Country Link
CN (1) CN108610182A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971680A (en) * 2019-03-29 2019-07-05 云南省微生物发酵工程研究中心有限公司 A kind of preparation and application of the complex micro organism fungicide with disease-resistant growth-promoting function
CN111138229A (en) * 2020-01-07 2020-05-12 上海交通大学 Preparation and application of beneficial trichoderma and bacillus coated fertilizer
CN111286473A (en) * 2019-12-20 2020-06-16 云南省微生物发酵工程研究中心有限公司 Agricultural microbial bacterium composite inoculant and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104877937A (en) * 2015-05-18 2015-09-02 青岛根源生物技术集团有限公司 Bacillus amyloliquefaciens bacterial fertilizer for promoting growth of capsicum annuum and preparation method and applications thereof
CN104894010A (en) * 2015-05-18 2015-09-09 青岛根源生物技术集团有限公司 Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof
KR20150119601A (en) * 2014-04-16 2015-10-26 (주) 에코텍씨드 Granulated fertilizer capable of controlling grass diseases and manufacturing method thereof
CN108249987A (en) * 2017-12-25 2018-07-06 云南云叶化肥股份有限公司 A kind of compound fertilizer containing active microorganism and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150119601A (en) * 2014-04-16 2015-10-26 (주) 에코텍씨드 Granulated fertilizer capable of controlling grass diseases and manufacturing method thereof
CN104877937A (en) * 2015-05-18 2015-09-02 青岛根源生物技术集团有限公司 Bacillus amyloliquefaciens bacterial fertilizer for promoting growth of capsicum annuum and preparation method and applications thereof
CN104894010A (en) * 2015-05-18 2015-09-09 青岛根源生物技术集团有限公司 Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof
CN108249987A (en) * 2017-12-25 2018-07-06 云南云叶化肥股份有限公司 A kind of compound fertilizer containing active microorganism and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109971680A (en) * 2019-03-29 2019-07-05 云南省微生物发酵工程研究中心有限公司 A kind of preparation and application of the complex micro organism fungicide with disease-resistant growth-promoting function
CN111286473A (en) * 2019-12-20 2020-06-16 云南省微生物发酵工程研究中心有限公司 Agricultural microbial bacterium composite inoculant and preparation method thereof
CN111138229A (en) * 2020-01-07 2020-05-12 上海交通大学 Preparation and application of beneficial trichoderma and bacillus coated fertilizer

Similar Documents

Publication Publication Date Title
CN109971680B (en) Preparation and application of compound microbial agent with disease-resistant and growth-promoting functions
CN104609997A (en) Manufacturing process and application method of ginseng bio-organic fertilizer
CN105085121B (en) A kind of composite microbiological fertilizer
CN103964946A (en) Special compound microbial fertilizer for paddy rice and preparation method thereof
CN108530232A (en) A kind of efficient biologic-organic fertilizer and preparation method thereof
CN106591193A (en) Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects
CN103518781B (en) Core bio-control bacteria and organic fertilizer containing the core bio-control bacteria
CN107493897A (en) The method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil
CN102731213A (en) Fertilizer for cultivation of tobacco, preparation method and application thereof
CN110100670A (en) A method of prevention and treatment crop in cruciferae clubroot
CN105294277A (en) Light matrix formula for camellia oleifera seedlings and preparation method thereof
CN105330420A (en) Biological function organic-inorganic compound fertilizer containing amino acid, humic acid and nicotine and preparation method of biological function organic-inorganic compound fertilizer
CN110143836A (en) Using phosphoric acid as regulating acid agent and the rice seedling-growth soil regulator of phosphorus source and its preparation method and application
CN104230418B (en) Method for preparing organic fertilizer by fermenting cattle manure
CN105622296A (en) Composite soil modifying agent and preparation method and application thereof
CN105036984A (en) Microbial organic fertilizer and application of microbial organic fertilizer to tobacco planting
CN108059575A (en) A kind of bio-feritlizer and preparation method
CN108610182A (en) A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function
CN110004082A (en) A kind of bacterium bacterial strain and application suitable for the regulation of industrialized agriculture nitrogen and phosphorus pollution
CN107382496A (en) A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity
CN115340968A (en) Novel application and method of pseudomonas spinosa, pseudomonas spinosa 21.1.9.2-14 and product thereof
CN107827488A (en) A kind of liquid-state composite bacterial microbe and preparation method thereof
CN107746303A (en) It is a kind of using xylose residue as decomposed matrix of raw material and its preparation method and application
CN104830706B (en) One plant of bacillusmusilaginosiengineering and the organic sustained-controll-release fertiliser of potato special bio
CN109206272A (en) The fertilizer formulations and its application method of continuous cropping vega soil are repaired in a kind of improvement

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20181002

RJ01 Rejection of invention patent application after publication