CN107129358A - A kind of complex microorganism preparations that crop root part can be promoted to develop - Google Patents

A kind of complex microorganism preparations that crop root part can be promoted to develop Download PDF

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Publication number
CN107129358A
CN107129358A CN201710327930.1A CN201710327930A CN107129358A CN 107129358 A CN107129358 A CN 107129358A CN 201710327930 A CN201710327930 A CN 201710327930A CN 107129358 A CN107129358 A CN 107129358A
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parts
bacillus
sulfate
develop
vermiculite
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孔祥宜
孙瑞
屈庆涛
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Beijing Zhongnong Fuyuan Biological Engineering Technology Co Ltd
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Beijing Zhongnong Fuyuan Biological Engineering Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

A kind of complex microorganism preparations that crop root part can be promoted to develop, the complex microorganism preparations that can especially promote wheat root to develop, including:13 parts of 13 parts of bacillus subtilis, 15 parts of Rhodopseudomonas palustris, 13 parts of rhizobium, 12 parts of gel-shaped series bacillus, 35 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And 3 10 parts of ammonium sulfate, 18 parts of potassium sulfate, 1.5 2.8 parts of fulvic acid, 0.1 3 parts of sucrose, 5 12 parts of Diammonium phosphate (DAP), 0.1 0.6 parts of boric acid, 0.01 0.8 parts of sodium chloride, 1.5 3.8 parts of chitosan, 5 20 parts of plant ash, 0.1 3 parts of corn flour, 0.1 3 parts of calcium carbonate, 0.01 0.6 parts of lauryl sodium sulfate, and 5 20 parts of vermiculite.The present invention promotes the normal development of root system, improves wheat ability resistant to lodging, promote the volume increase of wheat, output increased 8 15%.

Description

A kind of complex microorganism preparations that crop root part can be promoted to develop
Technical field
It is more particularly to a kind of to promote the composite microbial of wheat root development the present invention relates to plant growth field is improved Thing preparation.
Background technology
Wheat is one of most important crop in China's agricultural components, and the wide geographic area in North of Yangtze River is planted extensively, is The main product grain kind in the provinces such as Shandong, Hebei, Henan.
One big problem of wheat planting is Wheat lodging phenomenon, and early lodging and evening lodging can be divided into from the time, and wheat falls Volt phenomenon has a strong impact on wheat maturation, reduces mass of 1000 kernel, causes the underproduction.According to statistics, due to lodging factor, every square metre averagely subtracts 35kg or so is produced, wheat large area high yield, stable yields is directly affected.
How to solve the problems, such as lodging is the research engineering of a system, can from breeding, edaphic condition, irrigation conditions and Consider in terms of weather, but all there is the inferior positions such as time-consuming, effect is not good in these modes.Therefore Wheat lodging how is solved to ask Topic is a crucial research factor of wheat planting.
The content of the invention
The present invention is directed to Wheat lodging problem, proposes a kind of complex microorganism preparations that wheat root can be promoted to develop, The growth of wheat root is improved, and then promotes the lodging tolerance of root.
Technical scheme is as follows.
A kind of complex microorganism preparations that wheat root can be promoted to develop, in terms of parts by weight, including:
1-3 parts of bacillus subtilis, 1-5 parts of Rhodopseudomonas palustris, 1-3 parts of rhizobium, gel-shaped series bacillus 1-2 1-3 parts of part, 3-5 parts of bacillus amyloliquefaciens and Friedlander's bacillus;
And 3-10 parts of ammonium sulfate, 1-8 parts of potassium sulfate, 1.5-2.8 parts of fulvic acid, 0.1-3 parts of sucrose, Diammonium phosphate (DAP) 5-12 Part, 0.1-0.6 parts of boric acid, 0.01-0.8 parts of sodium chloride, 1.5-3.8 parts of chitosan, 5-20 parts of plant ash, 0.1-3 parts of corn flour, 0.1-3 parts of calcium carbonate, 0.01-0.6 parts of lauryl sodium sulfate, and 5-20 parts of vermiculite.
Preferably, 2 parts of bacillus subtilis, 2 parts of Rhodopseudomonas palustris, 1.8 parts of rhizobium, gel-shaped series bacillus 2 parts of 1.5 parts, 4 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And 5 parts of ammonium sulfate, 2 parts of potassium sulfate, fulvic acid 2 Part, 1.8 parts of sucrose, 6 parts of Diammonium phosphate (DAP), 0.4 part of boric acid, 0.06 part of sodium chloride, 2 parts of chitosan, 10 parts of plant ash, corn flour 2 Part, 2 parts of calcium carbonate, 0.1 part of lauryl sodium sulfate, and 18 parts of vermiculite.
A kind of preparation method of described complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:1-3 parts of bacillus subtilis, 1-5 parts of Rhodopseudomonas palustris, root 1-3 parts of 1-3 parts of knurl bacterium, 1-2 parts of gel-shaped series bacillus, 3-5 parts of bacillus amyloliquefaciens and Friedlander's bacillus;With And 3-10 parts of ammonium sulfate, 1-8 parts of potassium sulfate, 1.5-2.8 parts of fulvic acid, 0.1-3 parts of sucrose, 5-12 parts of Diammonium phosphate (DAP), boric acid 0.1-0.6 parts, 0.01-0.8 parts of sodium chloride, 1.5-3.8 parts of chitosan, 5-20 parts of plant ash, 0.1-3 parts of corn flour, calcium carbonate 0.1-3 parts, 0.01-0.6 parts of lauryl sodium sulfate, and 5-20 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are at 25-40 DEG C Under be stirred mixing, stand 1-10 hour progress initial fermentations, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 50-100 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 12-72 hours and is carried out at fermentation Reason, obtains material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 0.8-3mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
Through monitoring, viable bacteria total concentration number is more than 1,000,000,000/ml in material C.
The present invention achieves significant technique effect.
Wherein, the present invention use bacillus subtilis, Rhodopseudomonas palustris, rhizobium, gel-shaped series bacillus, Bacillus amyloliquefaciens and Friedlander's bacillus, above-mentioned strain are strain purchased in market.6 kinds of described strains serve association With the effect of synergy, the function restriction of single culture is overcome, the synergy of above-mentioned 6 kinds of strains is particularly suitable for strengthening wheat The development of root system, and then the lodging problem of wheat is alleviated, improve the yield of wheat.
In the present invention, used two-step fermentation processing, first by bacillus subtilis, Rhodopseudomonas palustris, rhizobium, Gel-shaped series bacillus, bacillus amyloliquefaciens, Friedlander's bacillus are in ammonium sulfate, potassium sulfate, fulvic acid, sucrose, phosphorus Sour two ammoniums carry out initial fermentation under conditions of existing, and improve the adaptability of strain;Afterwards the product after initial fermentation again with Carry out further fermentation process after material B mixing, the new old agent capability of strain is greatly improved, it is to avoid strain it is abnormal dead Die, improve the activity of strain, metabolite and then the development for improving wheat root.
In the present invention, Surfactant SDS is added in the nutrient solution of strain, strain is improved and exists Mobility during fermentation, increases effect of mass transmitting.
In the present invention, zymocyte liquid is mixed with vermiculite power, strain is adsorbed in the duct of vermiculite, improve strain into Motility rate and life-span, and due to there is substantial amounts of inorganic microelement in vermiculite, the activity of strain is improved, while these are micro Element is absorbed after soil is spilt into by wheat, and then improves wheat grain filling degree, improves yield.
The present invention can be effectively improved the micro-ecological environment of wheat root, promote beneficial microorganism growth and breeding, simultaneously Suppress and the harmful humic bacterium of killing or pest and disease damage, promote the normal development of root system, improve wheat ability resistant to lodging, promote The volume increase of wheat, output increased 8-15%.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment.
Embodiment 1
A kind of preparation method for the complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:3 parts of bacillus subtilis, 5 parts of Rhodopseudomonas palustris, rhizobium 3 parts of 3 parts, 2 parts of gel-shaped series bacillus, 5 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And ammonium sulfate 10 Part, 8 parts of potassium sulfate, 2.8 parts of fulvic acid, 3 parts of sucrose, 12 parts of Diammonium phosphate (DAP), 0.6 part of boric acid, 0.8 part of sodium chloride, chitosan 3.8 Part, 20 parts of plant ash, 3 parts of corn flour, 3 parts of calcium carbonate, 0.6 part of lauryl sodium sulfate, and 20 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are entered at 40 DEG C Row stirring mixing, stands 2 hours and carries out initial fermentation, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 100 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 70 hours and is carried out fermentation process, obtains To material C;Through monitoring, viable bacteria total concentration number is 1,100,000,000/ml in material C.
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 0.8mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
Embodiment 2
A kind of preparation method for the complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:1 part of bacillus subtilis, 1 part of Rhodopseudomonas palustris, rhizobium 1 part of 1 part, 1 part of gel-shaped series bacillus, 3 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And 3 parts of ammonium sulfate, 1 part of potassium sulfate, 1.5 parts of fulvic acid, 0.1 part of sucrose, 5 parts of Diammonium phosphate (DAP), 0.1 part of boric acid, 0.01 part of sodium chloride, chitosan 1.5 Part, 5 parts of plant ash, 0.1 part of corn flour, 0.1 part of calcium carbonate, 0.01 part of lauryl sodium sulfate, and 5 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are entered at 25 DEG C Row stirring mixing, stands 10 hours and carries out initial fermentation, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 50 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 20 hours and is carried out fermentation process, obtains To material C;Through monitoring, viable bacteria total concentration number is 1,200,000,000/ml in material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 1mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
Embodiment 3
A kind of preparation method for the complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:2 parts of bacillus subtilis, 2 parts of Rhodopseudomonas palustris, rhizobium 2 parts of 1.8 parts, 1.5 parts of gel-shaped series bacillus, 4 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And ammonium sulfate 5 parts, 2 parts of potassium sulfate, 2 parts of fulvic acid, 1.8 parts of sucrose, 6 parts of Diammonium phosphate (DAP), 0.4 part of boric acid, 0.06 part of sodium chloride, chitosan 2 Part, 10 parts of plant ash, 2 parts of corn flour, 2 parts of calcium carbonate, 0.1 part of lauryl sodium sulfate, and 18 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are entered at room temperature Row stirring mixing, stands 10 hours and carries out initial fermentation, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 80 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 72 hours and is carried out fermentation process, obtains To material C;Through monitoring, viable bacteria total concentration number is 1,800,000,000/ml in material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 0.8-3mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
Embodiment 4
A kind of preparation method for the complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:3 parts of bacillus subtilis, 5 parts of Rhodopseudomonas palustris, rhizobium 1 part of 3 parts, 2 parts of gel-shaped series bacillus, 3 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And 3 parts of ammonium sulfate, 1 part of potassium sulfate, 2.8 parts of fulvic acid, 3 parts of sucrose, 5 parts of Diammonium phosphate (DAP), 0.1 part of boric acid, 0.01 part of sodium chloride, 1.5 parts of chitosan, 5 parts of plant ash, 0.1 part of corn flour, 3 parts of calcium carbonate, 0.6 part of lauryl sodium sulfate, and 20 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are entered at 30 DEG C Row stirring mixing, stands 10 hours and carries out initial fermentation, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 100 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 72 hours and is carried out fermentation process, obtains To material C;Through monitoring, viable bacteria total concentration number is 1,300,000,000/ml in material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 2mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
Embodiment 5
A kind of preparation method for the complex microorganism preparations that wheat root can be promoted to develop includes:
Step (1), prepares each raw material by weight:2.1 parts of bacillus subtilis, 2.6 parts of Rhodopseudomonas palustris, root 2 parts of 1.8 parts of knurl bacterium, 1.5 parts of gel-shaped series bacillus, 4 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And sulphur Sour 10 parts of ammonium, 8 parts of potassium sulfate, 2.8 parts of fulvic acid, 3 parts of sucrose, 5 parts of Diammonium phosphate (DAP), 0.6 part of boric acid, 0.01 part of sodium chloride, shell 3.8 parts of glycan, 20 parts of plant ash, 3 parts of corn flour, 3 parts of calcium carbonate, 0.01 part of lauryl sodium sulfate, and 5 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped class gemma bar Bacterium, bacillus amyloliquefaciens, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are entered at 40 DEG C Row stirring mixing, stands 10 hours and carries out initial fermentation, obtain material A,;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, dodecyl sulphur Sour sodium is well mixed, and adds the sterilized water of 100 times of calcium carbonate consumption, after stirring, obtains material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 72 hours and is carried out fermentation process, obtains To material C;Through monitoring, viable bacteria total concentration number is 131,000,000,000/ml in material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, then in comminutor Then middle granulation, a diameter of 3mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
A kind of complex microorganism preparations that wheat root can be promoted to develop prepared for above-described embodiment 1-5, according to Different soil, product can be used alone or be used after being well mixed with organic fertilizer, for example, this product is by every mu 2-6 liters Ratio uniform is mixed into organic fertilizer administration.
Through overtesting, a kind of using the present invention can promote the complex microorganism preparations of wheat root development, disposably Addition is 5 kgs/acre, and the lodging tolerance of wheat significantly increases and decreases, output increased 8-15%.
Above example is used for illustrative purposes only, rather than limitation of the present invention, about the technology people of technical field Member, without departing from the spirit and scope of the present invention, can also make various conversion or modification, therefore all equivalent Technical scheme should also belong to scope of the invention, should be limited by each claim.

Claims (4)

1. a kind of complex microorganism preparations that wheat root can be promoted to develop, it is characterised in that in terms of parts by weight, including:
1-3 parts of bacillus subtilis, 1-5 parts of Rhodopseudomonas palustris, 1-3 parts of rhizobium, 1-2 parts of gel-shaped series bacillus, 1-3 parts of 3-5 parts of bacillus amyloliquefaciens and Friedlander's bacillus;
And 3-10 parts of ammonium sulfate, 1-8 parts of potassium sulfate, 1.5-2.8 parts of fulvic acid, 0.1-3 parts of sucrose, 5-12 parts of Diammonium phosphate (DAP), 0.1-0.6 parts of boric acid, 0.01-0.8 parts of sodium chloride, 1.5-3.8 parts of chitosan, 5-20 parts of plant ash, 0.1-3 parts of corn flour, carbon Sour calcium 0.1-3 parts, 0.01-0.6 parts of lauryl sodium sulfate, and 5-20 parts of vermiculite.
2. a kind of complex microorganism preparations that wheat root can be promoted to develop according to claim 1, it is characterised in that 2 parts of bacillus subtilis, 2 parts of Rhodopseudomonas palustris, 1.8 parts of rhizobium, 1.5 parts of gel-shaped series bacillus, solution starch bud 2 parts of 4 parts of spore bacillus and Friedlander's bacillus;And 5 parts of ammonium sulfate, 2 parts of potassium sulfate, 2 parts of fulvic acid, 1.8 parts of sucrose, phosphorus Sour 6 parts of two ammonium, 0.4 part of boric acid, 0.06 part of sodium chloride, 2 parts of chitosan, 10 parts of plant ash, 2 parts of corn flour, 2 parts of calcium carbonate, ten 0.1 part of sodium dialkyl sulfate, and 18 parts of vermiculite.
3. a kind of preparation method of complex microorganism preparations that wheat root can be promoted to develop described in claim 1, it is special Levy and be, including:
Step (1), prepares each raw material by weight:1-3 parts of bacillus subtilis, 1-5 parts of Rhodopseudomonas palustris, rhizobium 1-3 parts of 1-3 parts, 1-2 parts of gel-shaped series bacillus, 3-5 parts of bacillus amyloliquefaciens and Friedlander's bacillus;And sulphur Sour ammonium 3-10 parts, 1-8 parts of potassium sulfate, 1.5-2.8 parts of fulvic acid, 0.1-3 parts of sucrose, 5-12 parts of Diammonium phosphate (DAP), boric acid 0.1-0.6 Part, 0.01-0.8 parts of sodium chloride, 1.5-3.8 parts of chitosan, 5-20 parts of plant ash, 0.1-3 parts of corn flour, 0.1-3 parts of calcium carbonate, 0.01-0.6 parts of lauryl sodium sulfate, and 5-20 parts of vermiculite;
Step (2), by the bacillus subtilis of step (1), Rhodopseudomonas palustris, rhizobium, gel-shaped series bacillus, solution Bacillus amyloliquefacienses, Friedlander's bacillus, ammonium sulfate, potassium sulfate, fulvic acid, sucrose, Diammonium phosphate (DAP) are carried out at 25-40 DEG C Stirring mixing, stands 1-10 hours and carries out initial fermentation, obtain material A;
Step (3), by the boric acid of step (1), sodium chloride, chitosan, plant ash, corn flour, calcium carbonate, lauryl sodium sulfate It is well mixed, and the sterilized water of 50-100 times of calcium carbonate consumption is added, after stirring, obtain material B;
Step (4), material A is added gradually in material B, after stirring, and is stood 12-72 hours and is carried out fermentation process, obtains To material C;
Step (5), material C is mixed with step (1) vermiculite, in stirring, is freeze-dried, is then made in comminutor Then grain, a diameter of 0.8-3mm of granulated pellet preserves at drying, lucifuge, that is, obtains product.
4. preparation method according to claim 3, it is characterised in that viable bacteria total concentration number is more than 1,000,000,000/ml in material C.
CN201710327930.1A 2017-05-11 2017-05-11 A kind of complex microorganism preparations that crop root part can be promoted to develop Pending CN107129358A (en)

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WO2019142199A1 (en) * 2018-01-21 2019-07-25 Grace Breeding Nitrogen Fixation Technologies Ltd. Compositions containing combinations of nitrogen-fixing bacteria and additional agents and their use in fixing nitrogen in plant species
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