CN107998381B

CN107998381B - Compound preparation for preventing and treating white feces disease of penaeus vannamei boone and preparation and use methods thereof

Info

Publication number: CN107998381B
Application number: CN201711308737.XA
Authority: CN
Inventors: 苏汉沛
Original assignee: Hepu Junxiang Planting And Breeding Farmers' Professional Cooperative
Current assignee: Hepu Junxiang Planting And Breeding Farmers' Professional Cooperative
Priority date: 2017-12-11
Filing date: 2017-12-11
Publication date: 2021-09-07
Anticipated expiration: 2037-12-11
Also published as: CN107998381A

Abstract

The invention discloses a compound preparation for preventing and treating white feces disease of penaeus vannamei boone and a preparation method and a using method thereof, belonging to the technical field of cultivation, wherein the compound preparation comprises the following components in parts by weight: 3-5 parts of rhizoma atractylodis, 1-2 parts of elecampane, 0.5-1 part of pyroligneous, 0.01-0.1 part of lysozyme, 1-2 parts of chitosan, 5-8 parts of coptis chinensis, 3-6 parts of phellodendron, 5-10 parts of dried orange peel, 3-5 parts of liquorice and 2-4 parts of bighead atractylodes rhizome. The invention takes traditional Chinese medicines, wood vinegar, lysozyme and chitosan as raw materials, is safe and reliable in the process of treating the white feces of the prawns, has no medicine residue, does not cause pollution to water, and can improve the water quality.

Description

Compound preparation for preventing and treating white feces disease of penaeus vannamei boone and preparation and use methods thereof

Technical Field

The invention relates to the technical field of cultivation. More specifically, the invention relates to a compound preparation for preventing and treating white feces of penaeus vannamei boone, and a preparation method and a use method thereof.

Background

Many farmers have the condition of white feces in the process of cultivating prawns. Long-term rain causes prawn diseases to become complex and have high morbidity.

The main symptoms of enteritis caused by white feces are that shrimp bacteria are thin, white, sticky, float on the water surface, gather in a large amount and emit foul smell. The prawn ingests normally in the initial stage, but with the aggravation of the disease, the prawn eats slowly, even does not eat or is thin and weak, and the prawn swims to a pond and dies by throwing. Typical pathological changes of the intestinal tract include proliferation of submucosal cells, shedding of epithelial cells, and integral shedding of proliferated and fibrotic cell layers, which can be shed for many times to form white feces for many times.

At present, the white feces disease of prawns becomes one of the main diseases faced by the prawn feeding industry. The death rate is high after the disease occurs, the transmission is fast, and huge economic loss is easily caused. When the existing prawn white feces disease is treated, antibiotics are usually adopted, the effect is poor, side effects are easy to generate, and the water quality can be influenced.

Disclosure of Invention

The invention aims to provide a compound preparation for preventing and treating white feces disease of penaeus vannamei boone and a preparation and use method thereof, and the compound preparation without side effect is used for preventing and treating the white feces disease of the penaeus vannamei boone and has no pollution to water.

To achieve these objects and other advantages in accordance with the present invention, there is provided a complex formulation for controlling white feces disease of penaeus vannamei, comprising the following components in parts by weight:

3-5 parts of rhizoma atractylodis, 1-2 parts of elecampane, 0.5-1 part of pyroligneous, 0.01-0.1 part of lysozyme, 1-2 parts of chitosan, 5-8 parts of coptis chinensis, 3-6 parts of phellodendron, 5-10 parts of dried orange peel, 3-5 parts of liquorice and 2-4 parts of bighead atractylodes rhizome.

The preparation method of the compound preparation for preventing and treating white feces disease of penaeus vannamei boone comprises the following steps:

step one, respectively crushing and mixing 3-5 parts by weight of rhizoma atractylodis, 1-2 parts by weight of elecampane, 5-8 parts by weight of coptis chinensis, 3-6 parts by weight of golden cypress, 5-10 parts by weight of dried orange peel, 3-5 parts by weight of liquorice and 2-4 parts by weight of bighead atractylodes rhizome to obtain mixed powder;

step two, placing the mixed powder into water with the weight of 200 weight portions of 100-200 weight portions, adding 0.1-1 weight portion of cellulase and 0.1-1 weight portion of pectinase into the water, carrying out enzymolysis for 2-3h at 40-50 ℃ to obtain an enzymolysis liquid, then adding sucrose into the enzymolysis liquid to ensure that the concentration of the sucrose is 0.4-0.6g/mL, carrying out cell shrinkage to reach balance, quickly adding water to ensure that the concentration of the sucrose in the enzymolysis liquid is 0.05-0.1g/mL, then heating to 90-100 ℃, carrying out enzyme deactivation treatment for 30-60min, and filtering to obtain a first filtrate and first filter residue;

step three, sealing and fermenting the first filter residue at the temperature of 20-30 ℃ for 5-10 days, and filtering to obtain a second filtrate and a second filter residue;

and step four, mixing 0.5-1 part by weight of pyroligneous, 0.01-0.1 part by weight of lysozyme, 1-2 parts by weight of chitosan, the first filtrate and the second filtrate to obtain a mixed solution.

The use method of the compound preparation for preventing and treating white feces disease of penaeus vannamei boone comprises the following steps:

step one), drying the second filter residue, carrying out superfine grinding, mixing 0.5-1g of the second filter residue with each kilogram of feed, and taking the mixture once a day for 5-7 days continuously when the shrimps eat the mixture vigorously;

step two), sprinkling mixed liquor into the pond according to 180 g/mu of 150 plus materials 1-2 hours after the shrimps are eaten vigorously for 5-7 days.

Preferably, in the using method of the compound preparation for preventing and treating white feces of penaeus vannamei, in the second step), a plurality of spraying devices are adopted to spread the mixed solution into the pond;

the spraying device comprises:

the device comprises a shell, a feeding hole, a plurality of discharging holes, at least two pairs of ear plates, a plurality of sealing rings and a plurality of sealing rings, wherein the shell is cylindrical and is arranged along the vertical direction, the top of the shell is provided with the feeding hole, the bottom of the shell is provided with the plurality of discharging holes at intervals, the outer side wall of the shell is provided with at least two pairs of ear plates at intervals along the axial direction of the shell, and each pair of ear plates are symmetrically arranged relative to the axial line of the shell;

the baffle is annular and is arranged in the shell along the horizontal direction, the outer diameter of the baffle is equal to the inner diameter of the shell, and the outer ring of the baffle is fixed and connected with the inner side wall of the shell in a seamless manner;

the guide cylinder is cylindrical and is coaxially arranged with the shell, the top of the guide cylinder is fixedly connected with the top of the shell, the bottom of the guide cylinder penetrates through the middle of the partition plate and extends to the position below the partition plate without contacting with the bottom of the shell, a plurality of through holes are formed in the side wall of the guide cylinder at intervals, and the through holes are located between the top of the shell and the partition plate;

the sleeve is cylindrical and is sleeved on the guide cylinder, the inner diameter of the sleeve is equal to the outer diameter of the guide cylinder, and the outer diameter of the sleeve is equal to the inner diameter of the partition plate;

the power device is connected with the sleeve and is used for driving the sleeve to move up and down;

in the second step), the specific method for sprinkling the mixed liquid into the pond by adopting a plurality of sprinkling devices comprises the following steps:

set up a plurality of sprinkler along its width direction equidistant on the pond, wear to establish a wire rope in every sprinkler's otic placode, make wire rope extend along the length direction in pond, make the sleeve seal part or whole through-holes, later through leading-in mixed liquid in the feed inlet to the casing, make sprinkler at the uniform velocity along the length direction in pond remove, power device uniform velocity pushes down the sleeve simultaneously, mixed liquid sprays to the pond after through each discharge opening in.

Preferably, in the use method of the compound preparation for preventing and treating white feces of penaeus vannamei, the length of the sleeve is greater than the distance between the top of the shell and the partition plate.

Preferably, in the using method of the compound preparation for preventing and treating white feces of penaeus vannamei, the center of the bottom of the shell is gradually inclined downwards towards the edge.

The invention at least comprises the following beneficial effects:

the invention takes traditional Chinese medicines, wood vinegar, lysozyme and chitosan as raw materials, is safe and reliable in the process of treating the white feces of the prawns, has no medicine residue, and does not cause pollution to water.

The invention utilizes the filter residue and the filtrate of the traditional Chinese medicine, and can greatly save the cost while having high cure rate.

The invention adopts a plurality of spraying devices to spray at equal intervals, can ensure that the spraying is even everywhere, and avoids that part of the prawn is not cured and continues to be infected.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

Fig. 1 is a schematic structural view of a spraying apparatus according to an embodiment of the present invention.

Detailed Description

The present invention is further described in detail below with reference to the drawings and examples so that those skilled in the art can practice the invention with reference to the description.

It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials, if not otherwise specified, are commercially available; in the description of the present invention, the terms "lateral", "longitudinal", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Example 1

The compound preparation for preventing and treating white feces disease of penaeus vannamei boone comprises the following components in parts by weight:

3 parts of rhizoma atractylodis, 1 part of costustoot, 0.5 part of pyroligneous, 0.01 part of lysozyme, 1 part of chitosan, 5 parts of coptis chinensis, 3 parts of phellodendron, 5 parts of dried orange peel, 3 parts of liquorice and 2 parts of bighead atractylodes rhizome.

Cang Zhu for abdominal distention, diarrhea and edema.

Mu Xiang is indicated for abdominal distention and pain, belching, vomiting, diarrhea, dysentery, tenesmus, indigestion and poor appetite.

Coptidis rhizoma, for clearing heat and eliminating dampness, purging pathogenic fire and removing toxic substance, relieving distension and fullness due to damp-heat, emesis and acid regurgitation, and dysentery.

Huang Bai is indicated for dysentery due to damp-heat, jaundice and dark urine.

Chen Pi is indicated for gastric and abdominal distention, vomiting and diarrhea due to poor appetite.

Licorice root, radix Glycyrrhizae is indicated for stomach ache, abdominal pain and acute pain of gastrocnemius.

Atractylodis rhizoma, for invigorating spleen and qi, eliminating dampness and promoting diuresis, and treating spleen deficiency, anorexia, abdominal distention and diarrhea.

The wood vinegar has the functions of preventing bacteria, sterilizing, increasing drug effect and purifying water quality.

Lysozyme has the functions of antibiosis, antiphlogosis, antivirus, etc.

The chitosan has the functions of inhibiting bacterial activity, promoting blood coagulation and wound healing and purifying water quality.

By adopting the formula, the digestion and absorption functions of the prawns can be obviously improved, and the prawn feed has the effects of nourishing the stomach, resisting bacteria and improving water quality.

step one, respectively crushing and mixing 3 parts by weight of rhizoma atractylodis, 1 part by weight of costustoot, 5 parts by weight of coptis chinensis, 3 parts by weight of phellodendron amurense, 5 parts by weight of dried orange peel, 3 parts by weight of liquorice and 2 parts by weight of bighead atractylodes rhizome to obtain mixed powder;

step two, placing the mixed powder into 100 parts by weight of water, adding 0.1 part by weight of cellulase and 0.1 part by weight of pectinase (to destroy cell walls) into the water, carrying out enzymolysis for 2 hours at 40 ℃ to obtain an enzymolysis liquid, then adding sucrose (to destroy cell membranes) into the enzymolysis liquid to ensure that the concentration of the sucrose is 0.4g/mL, shrinking cells, rapidly adding water after balancing, ensuring that the concentration of the sucrose in the enzymolysis liquid is 0.05g/mL, then heating to 90 ℃, carrying out enzyme deactivation treatment for 30 minutes, and filtering to obtain a first filtrate and a first filter residue; through enzymolysis and placing in high-concentration sucrose solution, cell walls and cell membranes of cells can be damaged, and effective components in the cells can be dissolved out as much as possible.

Step three, sealing and fermenting the first filter residue at 20 ℃ for 5 days, and filtering to obtain a second filtrate and a second filter residue; the fermentation can further dissolve the effective substances in the cells. And small molecular substances are obtained, so that the second filter residue is easier to be absorbed by the prawns.

And step four, mixing 0.5 part by weight of pyroligneous, 0.01 part by weight of lysozyme, 1 part by weight of chitosan, the first filtrate and the second filtrate to obtain a mixed solution. The mixed solution has the effects of clearing heat, promoting appetite, killing gastrointestinal bacteria and purifying water.

step one), drying the second filter residue, carrying out superfine grinding, mixing 0.5g of the second filter residue with each kilogram of feed, and taking the mixture once a day for 7 days continuously when the shrimps eat the mixture vigorously;

step two), sprinkling mixed liquor into the pond according to 150 g/mu per meter 1 hour after the shrimps are eaten vigorously, and sprinkling for 7 days continuously.

In the using method of the compound preparation for preventing and treating white feces of the penaeus vannamei boone, in the second step), a plurality of spraying devices are adopted to spread mixed liquid into the pond;

as shown in fig. 1, the spraying apparatus includes:

the feeding device comprises a shell 100, a feeding hole 101, a plurality of discharging holes, at least two pairs of ear plates, a plurality of discharging holes and a plurality of discharging holes, wherein the shell 100 is cylindrical and is arranged along the vertical direction, the top of the shell 100 is provided with the feeding hole, the bottom of the shell 100 is provided with the discharging holes at intervals, the outer side wall of the shell 100 is provided with the at least two pairs of ear plates at intervals along the axial direction of the shell, and each pair of ear plates are symmetrically arranged relative to the axial line of the shell 100;

the partition plate 110 is annular and is arranged in the shell 100 along the horizontal direction, the outer diameter of the partition plate 110 is equal to the inner diameter of the shell 100, and the outer ring of the partition plate 110 is fixedly connected with the inner side wall of the shell 100 in a seamless mode;

the guide cylinder 120 is cylindrical and is coaxially arranged with the casing 100, the top of the guide cylinder 120 is fixedly connected with the top of the casing 100, the bottom of the guide cylinder 120 passes through the middle of the partition plate 110, extends to the lower part of the partition plate 110 and is not in contact with the bottom of the casing 100, and a plurality of through holes are arranged on the side wall of the guide cylinder 120 at intervals and are positioned between the top of the casing 100 and the partition plate 110;

a sleeve 130 having a cylindrical shape and fitted over the guide cylinder 120, wherein an inner diameter of the sleeve 130 is equal to an outer diameter of the guide cylinder 120, and an outer diameter of the sleeve 130 is equal to an inner diameter of the partition plate 110;

a power device connected to the sleeve 130 for driving the sleeve 130 to move up and down, such as an air cylinder or a hydraulic cylinder;

set up a plurality of sprinkler along its width direction equidistant on the pond, wear to establish a wire rope in every sprinkler's otic placode, make wire rope extend along the length direction in pond, make sleeve 130 seal part or whole through-hole (the middle part of baffle 110 is passed all the time to the bottom of sleeve 130, the addition volume of mixed liquid should be, the top surface of adding back mixed liquid is no longer than sleeve 130's top, later through leading-in mixed liquid in feed inlet 101 to casing 100, make sprinkler at the uniform velocity removal along the length direction in pond (for example set up the hoist engine on the bank, it removes to pull sprinkler through the hoist engine), power device uniform velocity pushes down sleeve 130 simultaneously, mixed liquid sprays to the pond after through each discharge opening in.

The spraying is carried out at equal intervals by a plurality of spraying devices, the spraying at each position can be ensured to be even, and the continuous infection caused by the uncured white feces of partial prawns is avoided.

In the use method of the compound preparation for preventing and treating white feces of penaeus vannamei, the length of the sleeve 130 is greater than the distance between the top of the shell 100 and the partition 110. This seals all of the holes when the top of the sleeve 130 contacts the top of the housing 100, allowing more of the mixture to be contained within the housing 100.

In the use method of the compound preparation for preventing and treating white feces of penaeus vannamei, the center of the bottom of the shell 100 is gradually inclined downwards towards the edge. Mixed liquid passes through the through-hole and gets into in the guide cylinder 120 back, comes out from the bottom of guide cylinder 120, flows to the middle part of the bottom of casing 100, and the bottom of rethread casing 100 disperses, and it is more even to enable mixed liquid to spray.

Example 2

4 parts of rhizoma atractylodis, 1.5 parts of costustoot, 0.75 part of pyroligneous, 0.05 part of lysozyme, 1.5 parts of chitosan, 6.5 parts of coptis chinensis, 4.5 parts of phellodendron, 7.5 parts of dried orange peel, 4 parts of liquorice and 3 parts of bighead atractylodes rhizome.

Cang Zhu for abdominal distention, diarrhea and edema.

Coptis root, rhizoma Coptidis, is used to clear heat and dry dampness, purge fire and remove toxicity, moisten and hot feeling and fullness, vomiting and acid regurgitation, dysentery.

Huang Bai is indicated for damp-heat dysentery, jaundice and dark urine.

Bai Zhu is indicated for invigorating spleen and qi, drying dampness and inducing diuresis, spleen deficiency and poor appetite, abdominal distension and diarrhea.

Lysozyme has the functions of antibiosis, antiphlogosis, antivirus, etc.

step one, respectively crushing and mixing 4 parts by weight of rhizoma atractylodis, 1.5 parts by weight of elecampane, 6.6 parts by weight of coptis chinensis, 4.5 parts by weight of golden cypress, 7.5 parts by weight of dried orange peel, 4 parts by weight of liquorice and 3 parts by weight of bighead atractylodes rhizome to obtain mixed powder;

step two, placing the mixed powder into 150 parts by weight of water, adding 0.6 part by weight of cellulase and 0.6 part by weight of pectinase into the water, carrying out enzymolysis for 2.5 hours at 45 ℃ to obtain an enzymolysis liquid, then adding sucrose into the enzymolysis liquid to ensure that the concentration of the sucrose is 0.5g/mL, shrinking cells, rapidly adding water to ensure that the concentration of the sucrose in the enzymolysis liquid is 0.07g/mL after balancing, then heating to 95 ℃, carrying out enzyme deactivation treatment for 45 minutes, and filtering to obtain a first filtrate and a first filter residue; through enzymolysis and placing in high-concentration sucrose solution, cell walls and cell membranes of cells can be damaged, and effective components in the cells can be dissolved out as much as possible.

Step three, sealing and fermenting the first filter residue at 25 ℃ for 7 days, and filtering to obtain a second filtrate and a second filter residue; the fermentation can further dissolve the effective substances in the cells. And small molecular substances are obtained, so that the second filter residue is easier to be absorbed by the prawns.

And step four, mixing 0.75 weight part of pyroligneous, 0.05 weight part of lysozyme, 1.5 weight parts of chitosan, the first filtrate and the second filtrate to obtain a mixed solution. The mixed solution has the effects of clearing heat, promoting appetite, killing gastrointestinal bacteria and purifying water.

step one), drying the second filter residue, carrying out superfine grinding, mixing 0.7g of the second filter residue with each kilogram of feed, and taking the mixture once a day for 7 days continuously when the shrimps eat the mixture vigorously;

step two), spraying mixed liquid into the pond according to 165 g/mu.m for 1.5 hours after the shrimps are eaten vigorously, and continuously spraying for 7 days.

the spraying device comprises:

the power device is connected with the sleeve and is used for driving the sleeve to move up and down, such as an air cylinder or a hydraulic cylinder;

In the use method of the compound preparation for preventing and treating white feces of the penaeus vannamei, the length of the sleeve is greater than the distance between the top of the shell and the partition plate. Therefore, when the top of the sleeve contacts the top of the shell, all the through holes can be sealed, and more mixed liquid can be contained in the shell.

In the use method of the compound preparation for preventing and treating white feces of the penaeus vannamei, the center of the bottom of the shell gradually inclines downwards towards the edge. Mixed liquid passes through the through-hole and gets into back in the guide cylinder, comes out from the bottom of guide cylinder, flows to the middle part of the bottom of casing, and the bottom of rethread casing disperses, enables mixed liquid and sprays more evenly.

Example 3

5 parts of rhizoma atractylodis, 2 parts of elecampane, 1 part of pyroligneous, 0.1 part of lysozyme, 2 parts of chitosan, 8 parts of coptis chinensis, 6 parts of phellodendron, 10 parts of dried orange peel, 5 parts of liquorice and 4 parts of bighead atractylodes rhizome.

Cang Zhu for abdominal distention, diarrhea and edema.

Huang Bai is indicated for damp-heat dysentery, jaundice and dark urine.

Lysozyme has the functions of antibiosis, antiphlogosis, antivirus, etc.

step one, respectively crushing and mixing 5 parts by weight of rhizoma atractylodis, 2 parts by weight of costustoot, 8 parts by weight of coptis chinensis, 6 parts by weight of phellodendron amurense, 10 parts by weight of dried orange peel, 5 parts by weight of liquorice and 4 parts by weight of bighead atractylodes rhizome to obtain mixed powder;

step two, placing the mixed powder into 200 parts by weight of water, adding 1 part by weight of cellulase and 1 part by weight of pectinase into the water, carrying out enzymolysis for 3 hours at 50 ℃ to obtain an enzymolysis liquid, then adding sucrose into the enzymolysis liquid to enable the concentration of the sucrose to be 0.6g/mL, shrinking cells, after balancing, rapidly adding water to enable the concentration of the sucrose in the enzymolysis liquid to be 0.1g/mL, then heating to 100 ℃, carrying out enzyme deactivation treatment for 60 minutes, and then filtering to obtain a first filtrate and a first filter residue; through enzymolysis and placing in high-concentration sucrose solution, cell walls and cell membranes of cells can be damaged, and effective components in the cells can be dissolved out as much as possible.

Step three, sealing and fermenting the first filter residue at 30 ℃ for 10 days, and filtering to obtain a second filtrate and a second filter residue; the fermentation can further dissolve the effective substances in the cells. And small molecular substances are obtained, so that the second filter residue is easier to be absorbed by the prawns.

And step four, mixing 1 part by weight of pyroligneous 0.1 part by weight of lysozyme, 2 parts by weight of chitosan, the first filtrate and the second filtrate to obtain a mixed solution. The mixed solution has the effects of clearing heat, promoting appetite, killing gastrointestinal bacteria and purifying water.

step one), drying the second filter residue, carrying out superfine grinding, mixing 1g of the second filter residue with each kilogram of feed, and taking the mixture once a day for 7 days continuously when the shrimps eat the mixture vigorously;

step two), sprinkling mixed liquor into the pond according to 180 g/mu.m for 2 hours after the shrimps are eaten vigorously, and sprinkling for 7 days continuously.

the spraying device comprises:

Comparative example 1

Decocting rhizoma Atractylodis 3 weight parts, radix aucklandiae 1 weight part, Coptidis rhizoma 5 weight parts, cortex Phellodendri 3 weight parts, pericarpium Citri Tangerinae 5 weight parts, Glycyrrhrizae radix 3 weight parts and Atractylodis rhizoma 2 weight parts in water, filtering, and sprinkling the filtrate 150 g/mu/m once a day for 7 times.

Comparative example 2

Decocting rhizoma Atractylodis 4 weight parts, radix aucklandiae 1.5 weight parts, Coptidis rhizoma 6.6 weight parts, cortex Phellodendri 4.5 weight parts, pericarpium Citri Tangerinae 7.5 weight parts, Glycyrrhrizae radix 4 weight parts and Atractylodis rhizoma 3 weight parts in water, filtering, and sprinkling the filtrate according to 165g total amount/mu/m once a day for 7 times.

Comparative example 3

Decocting rhizoma Atractylodis 5 weight parts, radix aucklandiae 2 weight parts, Coptidis rhizoma 8 weight parts, cortex Phellodendri 6 weight parts, pericarpium Citri Tangerinae 10 weight parts, Glycyrrhrizae radix 5 weight parts and Atractylodis rhizoma 4 weight parts in water, filtering, and sprinkling the filtrate 180 g/mu/m once a day for 7 times.

Test 1

After 7 months in certain areas in Guangxi, prawns have large-area white feces, and when the prawns are infected early, the prawns swim to the pond, the feed is reduced, slender viscous white feces are discharged, the prawns float on the water surface, the shrimp shells become soft, and when the prawns are severe, the pathological changes of the jejunum, the empty stomach and the liver and the pancreas occur. At the moment, the prawn size reaches 30 thousands of prawns/mu, 6 ponds with the same area and the same culture density and other culture modes are respectively controlled by the methods of the examples 1-3 and the comparative examples 1-3, and the control results are shown in the table 1.

TABLE 1 results of prevention and control of white feces by the methods of examples 1 to 3 and comparative examples 1 to 3, respectively

As can be seen from Table 1, the method of examples 1-3 can significantly reduce the mortality of the prawns, and after 5 days, the disease of the prawns is already alleviated, and the disease can be consolidated by continuously applying the method for 2 days, so that the disease does not relapse. Therefore, after the traditional Chinese medicinal materials are subjected to enzymolysis and are soaked by adopting a sucrose solution, more effective substances can be released, the drug effect can be improved, and the introduction of the wood vinegar, the lysozyme and the chitosan can further inhibit pathogenic bacteria in intestines and stomach of the prawns, improve the water quality and reduce the death rate of the prawns. The spraying device has the advantages that the medicine can be enabled to be effective, meanwhile, the spraying device sprays the medicine at equal intervals, the spraying at each position can be guaranteed to be even, and the situation that partial prawn white feces are not cured and are continuously infected is avoided.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details shown and described herein without departing from the generic concept as defined by the claims and their equivalents.

Claims

1. The preparation method of the compound preparation for preventing and treating white feces of penaeus vannamei boone is characterized by comprising the following steps of: