CN107998381A - Prevent the compound formulation and its preparation and application of Penaeus Vannmei white excrement disease - Google Patents
Prevent the compound formulation and its preparation and application of Penaeus Vannmei white excrement disease Download PDFInfo
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- CN107998381A CN107998381A CN201711308737.XA CN201711308737A CN107998381A CN 107998381 A CN107998381 A CN 107998381A CN 201711308737 A CN201711308737 A CN 201711308737A CN 107998381 A CN107998381 A CN 107998381A
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- 241000927735 Penaeus Species 0.000 title claims abstract description 35
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Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
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- A—HUMAN NECESSITIES
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A—HUMAN NECESSITIES
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses the compound formulation and its preparation and application of prevention Penaeus Vannmei white excrement disease, belong to cultural technique field, compound formulation includes the component of following parts by weight：24 parts of 35 parts of rhizoma atractylodis, 12 parts of the banksia rose, 0.5 1 parts of wood vinegar, 0.01 0.1 parts of lysozyme, 12 parts of chitosan, 58 parts of the coptis, 36 parts of Cortex Phellodendri, 5 10 parts of dried orange peel, 35 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.The present invention using Chinese medicine, wood vinegar, lysozyme and chitosan as raw material, treatment prawn in vain just during, securely and reliably, drug residue free, will not pollute water body, moreover it is possible to improve water quality.
Description
Technical field
The present invention relates to cultural technique field.A kind of it is more particularly related to prevention Penaeus Vannmei white excrement disease
Compound formulation and its preparation and application.
Background technology
There is the situation of " in vain just " during cultured prawn in many raisers.Long-term rainwater causes to prawn disease
Evil becomes complicated, and incidence is high.
In vain just the cardinal symptom of enteritis be the elongated white, toughness of shrimp bacterium, keep afloat, largely aggregation, foul smelling dissipates
Hair.Starting stage prawn ingests normally, but with aggravation, occurs that material feeding is slow, and not even material feeding or shrimp body is thin and weak and adjoint
Swim the pool and throw dead.Typical intestinal lesion be submucosa hyperplasia, epithelial cell shedding, hyperplasia simultaneously fibrosis it is thin
Born of the same parents' layer is overall to come off, and can repeatedly come off, formed repeatedly it is white just.
Prawn white excrement disease has become one of principal disease that prawn raising industry faces at present.The death rate is high after it is fallen ill,
Propagate soon, easily cause huge economic loss.Existing prawn white excrement disease is ineffective frequently with antibiotic in treatment, easily
Side effect is produced, and water quality can be influenced.
The content of the invention
The object of the present invention is to provide prevention Penaeus Vannmei white excrement disease compound formulation and its preparation and application, with
Prawn white excrement disease is prevented using compound formulation without side-effects, and it is pollution-free to water body.
In order to realize these purposes and further advantage according to the present invention, there is provided prevention Penaeus Vannmei white excrement disease is answered
Preparation is closed, includes the component of following parts by weight：
3-5 parts of rhizoma atractylodis, 1-2 parts of the banksia rose, 0.5-1 parts of wood vinegar, 0.01-0.1 parts of lysozyme, 1-2 parts of chitosan, coptis 5-
2-4 parts of 8 parts, 3-6 parts of Cortex Phellodendri, 5-10 parts of dried orange peel, 3-5 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.
The preparation method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, is comprised the following steps：
Step 1: by the rhizoma atractylodis of 3-5 parts by weight, the banksia rose of 1-2 parts by weight, the coptis of 5-8 parts by weight, 3-6 parts by weight
Cortex Phellodendri, the dried orange peel of 5-10 parts by weight, the Rhizoma Atractylodis Macrocephalae of the radix glycyrrhizae of 3-5 parts by weight and 2-4 parts by weight mix after crushing respectively, are mixed
Powder；
Step 2: after mixed-powder is placed in the water of 100-200 parts by weight, the fibre of 0.1-1 parts by weight is added into water
The pectase of the plain enzyme of dimension and 0.1-1 parts by weight, digests 2-3h at 40-50 DEG C, obtains enzymolysis liquid, backward enzymolysis liquid in add
Sucrose, the concentration for making sucrose is 0.4-0.6g/mL, and cell is shunk, and after reaching balance, quickly adds water, makes sugarcane in enzymolysis liquid
The concentration of sugar be 0.05-0.1g/mL, is warming up to 90-100 DEG C afterwards, after destroy the enzyme treatment 30-60min, filters, obtains the first filter
Liquid and the first filter residue；
Step 3: after the first filter residue is sealed by fermentation 5-10 days at 20-30 DEG C, filtering, obtains the second filtrate and second
Filter residue；
Step 4: the shell of the wood vinegar of 0.5-1 parts by weight, the lysozyme of 0.01-0.1 parts by weight, 1-2 parts by weight is gathered
Sugar, the first filtrate and the mixing of the second filtrate, obtain mixed liquor.
The application method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, including：
Step 1), by after the second filter residue and drying, carry out ultramicro grinding, mixed by per kilogram feed and take the second filter residues of 0.5-1g,
Eat food the vigorous time in shrimp, once a day, be used in conjunction 5-7 days；
Step 2), the 1-2h after shrimp eats food vigorous time, by the 150-180g/ mus meters mixed liquors unreasonable like a shrew into pond,
Company spreads 5-7 days.
Preferably, in the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the step 2)
In, using multiple flushers into pond mixed liquor unreasonable like a shrew；
The flusher includes：
Housing, it is cylindrical, and is vertically set, and is provided with feed inlet at the top of the housing, between bottom
Every being provided with multiple discharge holes, at least two pairs of otic placodes, each pair are arranged at intervals with along its axis direction on the lateral wall of the housing
Otic placode is set relative to the axisymmetrical of housing；
Partition plate, it is circular ring shape, and is arranged horizontally in the housing, the outside diameter of partition plate and the internal diameter phase of housing
Deng the outer ring of partition plate and the madial wall of housing are fixed and be seamlessly connected；
Guide cylinder, it is the cylinder bodily form, and is coaxially disposed with the housing, the top of the guide cylinder and the housing
Top is fixedly connected, and the bottom of the guide cylinder passes through the middle part of the partition plate, and extends to the lower section of the partition plate, and and shell
The bottom of body does not contact, and multiple through holes are arranged at intervals with the side wall of the guide cylinder, and the multiple through hole is located at the housing
Top and the partition plate between；
Sleeve, it is the cylinder bodily form, and is set on the guide cylinder, and the internal diameter of the sleeve is outer with the guide cylinder
Footpath is equal, and the outside diameter of the sleeve is equal with the internal diameter of the partition plate；
Power set, itself and the sleeve connection, for driving the sleeve to move up and down；
The step 2) in, use the specific method of multiple flushers from mixed liquor unreasonable like a shrew to pond for：
Multiple flushers are set at equal intervals along its width on pond, are worn in the otic placode of each flusher
Steel cable, makes steel wire rope extend along the length direction in pond, sleeve is sealed part or all of through hole, pass through charging afterwards
Mouth imports mixed liquor into housing, flusher is at the uniform velocity moved along the length direction in pond, while power set average rate is downward
Sleeve is promoted, mixed liquor after each discharge hole by being sprayed in pond.
Preferably, in the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the sleeve
Length is more than the top of the housing and the distance of the partition room.
Preferably, in the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the housing
The center of bottom tilts gradually downward towards edge.
The present invention includes at least following beneficial effect：
The present invention using Chinese medicine, wood vinegar, lysozyme and chitosan as raw material, treatment prawn in vain just during, safely may be used
Lean on, drug residue free, water body will not be polluted.
The present invention is all utilized the filter residue of Chinese medicine and filtrate, while cure rate is high, can also be greatlyd save
Cost.
The present invention is equally spaced sprayed using multiple flushers, can guarantee that sprinkling is uniform everywhere, avoids part right
Shrimp is not just cured in vain, and continues to infect.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 is the structure diagram of flusher according to an embodiment of the invention.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples, to make those skilled in the art's reference
Specification word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained；In the description of the present invention, term " transverse direction ", " vertical
To ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", the instruction such as " outer " side
Position or position relationship are based on orientation shown in the drawings or position relationship, are for only for ease of the description present invention and simplify description,
It is not instruction or implies signified device or element there must be specific orientation, with specific azimuth configuration and operation, because
This is not considered as limiting the invention.
Embodiment 1
The compound formulation of Penaeus Vannmei white excrement disease is prevented, includes the component of following parts by weight：
It is 3 parts of rhizoma atractylodis, 1 part of the banksia rose, 0.5 part of wood vinegar, 0.01 part of lysozyme, 1 part of chitosan, 5 parts of the coptis, 3 parts of Cortex Phellodendri, old
2 parts of 5 parts of skin, 3 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.
Storehouse art, for abdominal fullness and distention, diarrhea, oedema.
The banksia rose, for abdominal distention, spits weight, dyspepsia, do not feel like eating after diarrhea, dysentery.
The coptis, for heat-clearing and damp-drying drug, purging intense heat and detonicating, damp and hot feeling of fullness, vomiting acid regurgitation, rush down dysentery.
Cortex Phellodendri, for damp-heat dysentery, jaundice.
Dried orange peel, vomits and diarrhoea for abdominal fullness and distention, deficiency of food.
Radix glycyrrhizae, for having a stomachache, abdominal pain and gastrocnemius contraction urgency pain.
Rhizoma Atractylodis Macrocephalae, for strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, spleen eating less, abdominal distention diarrhea.
Wood vinegar, plays fungi-proofing sterilization, increases drug effect, the effect to purify water.
Lysozyme, has the effect such as antibacterial, anti-inflammatory, antiviral.
Chitosan, has and inhibits bacteria activity, promotes blood coagulation and wound healing, the effect to purify water.
Using above-mentioned formula, the digestion and absorption function of prawn can be obviously improved, and there is nourishing the stomach, the effect of Anti-bacterium, also
Water quality can be improved.
The preparation method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, is comprised the following steps：
Step 1: by the rhizoma atractylodis of 3 parts by weight, the banksia rose of 1 parts by weight, the coptis of 5 parts by weight, the Cortex Phellodendri of 3 parts by weight, 5 weights
The dried orange peel, the radix glycyrrhizae of 3 parts by weight and the Rhizoma Atractylodis Macrocephalae of 2 parts by weight of amount part mix after crushing respectively, obtain mixed-powder；
Step 2: after mixed-powder is placed in the water of 100 parts by weight, the cellulase of 0.1 parts by weight is added into water
With the pectase (destroy cell membrane) of 0.1 parts by weight, digest 2h at 40 DEG C, obtain enzymolysis liquid, backward enzymolysis liquid in plus
Entering sucrose (destroy cell membrane), the concentration for making sucrose is 0.4g/mL, and cell is shunk, and after reaching balance, quickly adds water,
The concentration for making sucrose in enzymolysis liquid is 0.05g/mL, is warming up to 90 DEG C afterwards, and after destroy the enzyme treatment 30min, filtering, obtains the first filter
Liquid and the first filter residue；By digesting and being placed in high concentration sucrose solution, the cell membrane and cell membrane of cell can be destroyed, makes cell
Interior active ingredient is dissolved out as much as possible.
Step 3: after the first filter residue is sealed by fermentation 5 days at 20 DEG C, filtering, obtains the second filtrate and the second filter residue；Hair
Ferment can further be such that intracellular active principle is fully dissolved out.And small-molecule substance is obtained, it is easier the second filter residue
Absorbed by prawn.
Step 4: the wood vinegar of 0.5 parts by weight, the lysozyme of 0.01 parts by weight, the chitosan of 1 parts by weight, first are filtered
Liquid and the mixing of the second filtrate, obtain mixed liquor.Mixed liquor has effects that heat-clearing, appetitive, moreover it is possible to kills stomach bacterium, only
Change water quality.
The application method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, including：
Step 1), by after the second filter residue and drying, carry out ultramicro grinding, mixed by per kilogram feed and take the second filter residues of 0.5g,
Shrimp eats food the vigorous time, once a day, is used in conjunction 7 days；
Step 2), the 1h after shrimp eats food vigorous time, by the 150g/ mu meters mixed liquors unreasonable like a shrew into pond, even spread 7
My god.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the step 2) in, using more
A flusher mixed liquor unreasonable like a shrew into pond；
As shown in Figure 1, the flusher includes：
Housing 100, it is cylindrical, and is vertically set, and the top of the housing 100 is provided with feed inlet
101, bottom interval is provided with multiple discharge holes, is arranged at intervals with least along its axis direction on the lateral wall of the housing 100
Two pairs of otic placodes, each pair otic placode are set relative to the axisymmetrical of housing 100；
Partition plate 110, it is circular ring shape, and is arranged horizontally in the housing 100, the outside diameter and shell of partition plate 110
The internal diameter of body 100 is equal, and the outer ring of partition plate 110 and the madial wall of housing 100 are fixed and be seamlessly connected；
Guide cylinder 120, it is the cylinder bodily form, and is coaxially disposed with the housing 100, the top of the guide cylinder 120 with
The top of the housing 100 is fixedly connected, and the bottom of the guide cylinder 120 passes through the middle part of the partition plate 110, and extends to institute
The lower section of partition plate 110 is stated, and is not contacted with the bottom of housing 100, is arranged at intervals with the side wall of the guide cylinder 120 multiple logical
Hole, the multiple through hole are located between top and the partition plate 110 of the housing 100；
Sleeve 130, it is the cylinder bodily form, and is set on the guide cylinder 120, the internal diameter of the sleeve 130 with it is described
The outside diameter of guide cylinder 120 is equal, and the outside diameter of the sleeve 130 is equal with the internal diameter of the partition plate 110；
Power set, it is connected with the sleeve 130, for driving the sleeve 130 to move up and down, such as cylinder or liquid
Cylinder pressure；
The step 2) in, use the specific method of multiple flushers from mixed liquor unreasonable like a shrew to pond for：
Multiple flushers are set at equal intervals along its width on pond, are worn in the otic placode of each flusher
Steel cable, make steel wire rope along pond length direction extend, make sleeve 130 seal part or all of through hole (sleeve 130
Bottom passes through the middle part of partition plate 110 all the time, and the addition of mixed liquor should be, and the top surface of mixed liquor is no more than sleeve 130 after addition
Top), mixed liquor is imported into housing 100 by feed inlet 101 afterwards, make flusher along pond length direction at the uniform velocity
Mobile (such as hoist engine being set on bank, move by elevator machine travel flusher), while power set average rate pushes down on
Moving sleeve 130, mixed liquor after each discharge hole by being sprayed in pond.
Equally spaced sprayed by multiple flushers, can guarantee that sprinkling is uniform everywhere, avoid part prawn in vain just
Do not cure, and continue to infect.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the length of the sleeve 130 is big
Distance between the top of the housing 100 and the partition plate 110.So sleeve 130 top contact housing 100 top
When, all through holes can be sealed, 100 interior energy of housing is contained more mixed liquors.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the bottom of the housing 100
Center tilts gradually downward towards edge.After mixed liquor is entered in guide cylinder 120 by through hole, go out from the bottom of guide cylinder 120
Come, flow to the middle part of the bottom of housing 100, then disperseed by the bottom of housing 100, can spray mixed liquor more equal
It is even.
Embodiment 2
The compound formulation of Penaeus Vannmei white excrement disease is prevented, includes the component of following parts by weight：
4 parts of rhizoma atractylodis, 1.5 parts of the banksia rose, 0.75 part of wood vinegar, 0.05 part of lysozyme, 1.5 parts of chitosan, 6.5 parts of the coptis, Huang
3 parts of 4.5 parts of cypress, 7.5 parts of dried orange peel, 4 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.
Storehouse art, for abdominal fullness and distention, diarrhea, oedema.
The banksia rose, for abdominal distention, spits weight, dyspepsia, do not feel like eating after diarrhea, dysentery.
The coptis, for heat-clearing and damp-drying drug, purging intense heat and detonicating, damp and hot feeling of fullness, vomits acid regurgitation, rush down dysentery.
Cortex Phellodendri, for damp-heat dysentery, jaundice.
Dried orange peel, for abdominal fullness and distention, deficiency of food is vomited and diarrhoea.
Radix glycyrrhizae, for having a stomachache, abdominal pain and gastrocnemius contraction urgency pain.
Rhizoma Atractylodis Macrocephalae, for strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, spleen eating less, abdominal distention diarrhea.
Wood vinegar, plays fungi-proofing sterilization, increases drug effect, the effect to purify water.
Lysozyme, has the effect such as antibacterial, anti-inflammatory, antiviral.
Chitosan, has and inhibits bacteria activity, promotes blood coagulation and wound healing, the effect to purify water.
Using above-mentioned formula, the digestion and absorption function of prawn can be obviously improved, and there is nourishing the stomach, the effect of Anti-bacterium, also
Water quality can be improved.
The preparation method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, is comprised the following steps：
Step 1: by the rhizoma atractylodis of 4 parts by weight, the banksia rose of 1.5 parts by weight, the coptis of 6.6 parts by weight, 4.5 parts by weight Huang
Cypress, the dried orange peel of 7.5 parts by weight, the Rhizoma Atractylodis Macrocephalae of the radix glycyrrhizae of 4 parts by weight and 3 parts by weight mix after crushing respectively, obtain mixed-powder；
Step 2: after mixed-powder is placed in the water of 150 parts by weight, the cellulase of 0.6 parts by weight is added into water
With the pectase of 0.6 parts by weight, digest 2.5h at 45 DEG C, obtain enzymolysis liquid, backward enzymolysis liquid in add sucrose, make sucrose
Concentration be 0.5g/mL, cell is shunk, and after reaching balance, quickly plus water, the concentration for making sucrose in enzymolysis liquid is 0.07g/
ML, is warming up to 95 DEG C afterwards, and after destroy the enzyme treatment 45min, filtering, obtains the first filtrate and the first filter residue；By digesting and being placed in
In high concentration sucrose solution, the cell membrane and cell membrane of cell can be destroyed, makes intracellular active ingredient dissolve as much as possible
Come.
Step 3: after the first filter residue is sealed by fermentation 7 days at 25 DEG C, filtering, obtains the second filtrate and the second filter residue；Hair
Ferment can be dissolved out further intracellular active principle.And small-molecule substance is obtained, it is easier the second filter residue right
Shrimp absorbs.
Step 4: by the wood vinegar of 0.75 parts by weight, the lysozyme of 0.05 parts by weight, the chitosan of 1.5 parts by weight, first
Filtrate and the mixing of the second filtrate, obtain mixed liquor.Mixed liquor has effects that heat-clearing, appetitive, moreover it is possible to stomach bacterium is killed,
Purify water.
The application method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, including：
Step 1), by after the second filter residue and drying, carry out ultramicro grinding, mixed by per kilogram feed and take the second filter residues of 0.7g,
Shrimp eats food the vigorous time, once a day, is used in conjunction 7 days；
Step 2), the 1.5h after shrimp eats food vigorous time, by the 165g/ mu meters mixed liquors unreasonable like a shrew into pond, even spread 7
My god.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the step 2) in, using more
A flusher mixed liquor unreasonable like a shrew into pond；
The flusher includes：
Housing, it is cylindrical, and is vertically set, and is provided with feed inlet at the top of the housing, between bottom
Every being provided with multiple discharge holes, at least two pairs of otic placodes, each pair are arranged at intervals with along its axis direction on the lateral wall of the housing
Otic placode is set relative to the axisymmetrical of housing；
Partition plate, it is circular ring shape, and is arranged horizontally in the housing, the outside diameter of partition plate and the internal diameter phase of housing
Deng the outer ring of partition plate and the madial wall of housing are fixed and be seamlessly connected；
Guide cylinder, it is the cylinder bodily form, and is coaxially disposed with the housing, the top of the guide cylinder and the housing
Top is fixedly connected, and the bottom of the guide cylinder passes through the middle part of the partition plate, and extends to the lower section of the partition plate, and and shell
The bottom of body does not contact, and multiple through holes are arranged at intervals with the side wall of the guide cylinder, and the multiple through hole is located at the housing
Top and the partition plate between；
Sleeve, it is the cylinder bodily form, and is set on the guide cylinder, and the internal diameter of the sleeve is outer with the guide cylinder
Footpath is equal, and the outside diameter of the sleeve is equal with the internal diameter of the partition plate；
Power set, itself and the sleeve connection, for driving the sleeve to move up and down, such as cylinder or hydraulic cylinder；
The step 2) in, use the specific method of multiple flushers from mixed liquor unreasonable like a shrew to pond for：
Multiple flushers are set at equal intervals along its width on pond, are worn in the otic placode of each flusher
Steel cable, makes steel wire rope extend along the length direction in pond, sleeve is sealed part or all of through hole, pass through charging afterwards
Mouth imports mixed liquor into housing, flusher is at the uniform velocity moved along the length direction in pond, while power set average rate is downward
Sleeve is promoted, mixed liquor after each discharge hole by being sprayed in pond.
Equally spaced sprayed by multiple flushers, can guarantee that sprinkling is uniform everywhere, avoid part prawn in vain just
Do not cure, and continue to infect.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the length of the sleeve is more than institute
State the top of housing and the distance of the partition room.So at the top of the top contact housing of sleeve, it can seal all
Through hole, makes housing interior energy contain more mixed liquors.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the center of the bottom of the housing
Tilted gradually downward towards edge.After mixed liquor is entered in guide cylinder by through hole, come out from the bottom of guide cylinder, flow to housing
Bottom middle part, then disperseed by the bottom of housing, mixed liquor sprinkling ground can be made evenly.
Embodiment 3
The compound formulation of Penaeus Vannmei white excrement disease is prevented, includes the component of following parts by weight：
5 parts of rhizoma atractylodis, 2 parts of the banksia rose, 1 part of wood vinegar, 0.1 part of lysozyme, 2 parts of chitosan, 8 parts of the coptis, 6 parts of Cortex Phellodendri, dried orange peel
4 parts of 10 parts, 5 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.
Storehouse art, for abdominal fullness and distention, diarrhea, oedema.
The banksia rose, for abdominal distention, spits weight, dyspepsia, do not feel like eating after diarrhea, dysentery.
The coptis, for heat-clearing and damp-drying drug, purging intense heat and detonicating, damp and hot feeling of fullness, vomits acid regurgitation, rush down dysentery.
Cortex Phellodendri, for damp-heat dysentery, jaundice.
Dried orange peel, for abdominal fullness and distention, deficiency of food is vomited and diarrhoea.
Radix glycyrrhizae, for having a stomachache, abdominal pain and gastrocnemius contraction urgency pain.
Rhizoma Atractylodis Macrocephalae, for strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, spleen eating less, abdominal distention diarrhea.
Wood vinegar, plays fungi-proofing sterilization, increases drug effect, the effect to purify water.
Lysozyme, has the effect such as antibacterial, anti-inflammatory, antiviral.
Chitosan, has and inhibits bacteria activity, promotes blood coagulation and wound healing, the effect to purify water.
Using above-mentioned formula, the digestion and absorption function of prawn can be obviously improved, and there is nourishing the stomach, the effect of Anti-bacterium, also
Water quality can be improved.
The preparation method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, is comprised the following steps：
Step 1: by the rhizoma atractylodis of 5 parts by weight, the banksia rose of 2 parts by weight, the coptis of 8 parts by weight, the Cortex Phellodendri of 6 parts by weight, 10 weights
The dried orange peel, the radix glycyrrhizae of 5 parts by weight and the Rhizoma Atractylodis Macrocephalae of 4 parts by weight of amount part mix after crushing respectively, obtain mixed-powder；
Step 2: after mixed-powder is placed in the water of 200 parts by weight, the cellulase and 1 of 1 parts by weight is added into water
The pectase of parts by weight, digests 3h at 50 DEG C, obtains enzymolysis liquid, backward enzymolysis liquid in add sucrose, make the concentration of sucrose
For 0.6g/mL, cell is shunk, and after reaching balance, quickly adds water, the concentration for making sucrose in enzymolysis liquid is 0.1g/mL, afterwards
It is warming up to 100 DEG C, after destroy the enzyme treatment 60min, filtering, obtains the first filtrate and the first filter residue；By digesting and being placed in high concentration
In sucrose solution, the cell membrane and cell membrane of cell can be destroyed, intracellular active ingredient is dissolved out as much as possible.
Step 3: after the first filter residue is sealed by fermentation 10 days at 30 DEG C, filtering, obtains the second filtrate and the second filter residue；
Fermentation energy is further dissolved out intracellular active principle.And small-molecule substance is obtained, the second filter residue is easier quilt
Prawn absorbs.
Step 4: by the lysozyme of 0.1 parts by weight of wood vinegar of 1 parts by weight, the chitosan of 2 parts by weight, the first filtrate and
Second filtrate mixes, and obtains mixed liquor.Mixed liquor has effects that heat-clearing, appetitive, moreover it is possible to kills stomach bacterium, purified water
Matter.
The application method of the compound formulation of Penaeus Vannmei white excrement disease is prevented, including：
Step 1), by after the second filter residue and drying, carry out ultramicro grinding, mixed by per kilogram feed and take the second filter residues of 1g, in shrimp
Eat food the vigorous time, once a day, be used in conjunction 7 days；
Step 2), the 2h after shrimp eats food vigorous time, by the 180g/ mu meters mixed liquors unreasonable like a shrew into pond, even spread 7
My god.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the step 2) in, using more
A flusher mixed liquor unreasonable like a shrew into pond；
The flusher includes：
Housing, it is cylindrical, and is vertically set, and is provided with feed inlet at the top of the housing, between bottom
Every being provided with multiple discharge holes, at least two pairs of otic placodes, each pair are arranged at intervals with along its axis direction on the lateral wall of the housing
Otic placode is set relative to the axisymmetrical of housing；
Partition plate, it is circular ring shape, and is arranged horizontally in the housing, the outside diameter of partition plate and the internal diameter phase of housing
Deng the outer ring of partition plate and the madial wall of housing are fixed and be seamlessly connected；
Guide cylinder, it is the cylinder bodily form, and is coaxially disposed with the housing, the top of the guide cylinder and the housing
Top is fixedly connected, and the bottom of the guide cylinder passes through the middle part of the partition plate, and extends to the lower section of the partition plate, and and shell
The bottom of body does not contact, and multiple through holes are arranged at intervals with the side wall of the guide cylinder, and the multiple through hole is located at the housing
Top and the partition plate between；
Sleeve, it is the cylinder bodily form, and is set on the guide cylinder, and the internal diameter of the sleeve is outer with the guide cylinder
Footpath is equal, and the outside diameter of the sleeve is equal with the internal diameter of the partition plate；
Power set, itself and the sleeve connection, for driving the sleeve to move up and down, such as cylinder or hydraulic cylinder；
The step 2) in, use the specific method of multiple flushers from mixed liquor unreasonable like a shrew to pond for：
Multiple flushers are set at equal intervals along its width on pond, are worn in the otic placode of each flusher
Steel cable, makes steel wire rope extend along the length direction in pond, sleeve is sealed part or all of through hole, pass through charging afterwards
Mouth imports mixed liquor into housing, flusher is at the uniform velocity moved along the length direction in pond, while power set average rate is downward
Sleeve is promoted, mixed liquor after each discharge hole by being sprayed in pond.
Equally spaced sprayed by multiple flushers, can guarantee that sprinkling is uniform everywhere, avoid part prawn in vain just
Do not cure, and continue to infect.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the length of the sleeve is more than institute
State the top of housing and the distance of the partition room.So at the top of the top contact housing of sleeve, it can seal all
Through hole, makes housing interior energy contain more mixed liquors.
In the application method of the compound formulation of the prevention Penaeus Vannmei white excrement disease, the center of the bottom of the housing
Tilted gradually downward towards edge.After mixed liquor is entered in guide cylinder by through hole, come out from the bottom of guide cylinder, flow to housing
Bottom middle part, then disperseed by the bottom of housing, mixed liquor sprinkling ground can be made evenly.
Comparative example 1
By the rhizoma atractylodis of 3 parts by weight, the banksia rose of 1 parts by weight, the coptis of 5 parts by weight, the Cortex Phellodendri of 3 parts by weight, 5 parts by weight it is old
After the Rhizoma Atractylodis Macrocephalae of skin, the radix glycyrrhizae of 3 parts by weight and 2 parts by weight endures water, filtering, filtrate is splashed according to 150 grams of total amount/mu/rice, daily
Once, continuous 7 times.
Comparative example 2
By the rhizoma atractylodis of 4 parts by weight, the banksia rose of 1.5 parts by weight, the coptis of 6.6 parts by weight, the Cortex Phellodendri of 4.5 parts by weight, 7.5 weights
After the dried orange peel, the radix glycyrrhizae of 4 parts by weight and the Rhizoma Atractylodis Macrocephalae of 3 parts by weight of amount part endure water, filtering, by filtrate according to 165 grams of total amount/mu/rice
Splash, once a day, continuous 7 times.
Comparative example 3
By the rhizoma atractylodis of 5 parts by weight, the banksia rose of 2 parts by weight, the coptis of 8 parts by weight, the Cortex Phellodendri of 6 parts by weight, 10 parts by weight
After the Rhizoma Atractylodis Macrocephalae of dried orange peel, the radix glycyrrhizae of 5 parts by weight and 4 parts by weight endures water, filtering, filtrate is splashed according to 180 grams of total amount/mu/rice, often
It once, continuous 7 times.
Experiment 1
After Guangxi somewhere July, prawn breaks out the white excrement disease of large area, during early infection, the prawn trip pool, and material reducing, row
Going out elongated stickiness white excrement, float on the water surface, shrimp shell softens, when serious, jejunum sky stomach, hepatopancrease lesion.Prawn is advised at this time
Lattice respectively support 6 area equations, cultivation density with other by the method for embodiment 1-3 and comparative example 1-3 up to 300,000 tails/mu
Grow the identical pond of mode to be prevented, prevention the results are shown in Table 1.
Table 1 prevents white result just respectively using the method for embodiment 1-3 and comparative example 1-3
As shown in Table 1, the death rate of prawn, and the prawn state of an illness after 5 days can be significantly reduced using the method for embodiment 1-3
Have been obtained for alleviating, the state of an illness can be consolidated by continuing to apply 2 days, make the state of an illness no longer recur.Thus illustrate by Chinese medicine into
After row is digested and soaked using sucrose solution, more active principles can be discharged, drug effect, wood vinegar, bacteriolyze can be increased
The introducing of enzyme and chitosan, can further suppress the germ in prawn stomach, and improve water quality, reduce the death rate of prawn.
While ensureing that medicine can play efficient, equally spaced sprayed by multiple flushers, can guarantee that sprinkling is equal everywhere
It is even, avoid part prawn from just not curing in vain, and continue to infect.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the legend and embodiment with description.
Claims (6)
1. prevent the compound formulation of Penaeus Vannmei white excrement disease, it is characterised in that include the component of following parts by weight：
3-5 parts of rhizoma atractylodis, 1-2 parts of the banksia rose, 0.5-1 parts of wood vinegar, 0.01-0.1 parts of lysozyme, 1-2 parts of chitosan, 5-8 parts of the coptis,
2-4 parts of 3-6 parts of Cortex Phellodendri, 5-10 parts of dried orange peel, 3-5 parts of radix glycyrrhizae and Rhizoma Atractylodis Macrocephalae.
2. prevent the preparation method of the compound formulation of Penaeus Vannmei white excrement disease, it is characterised in that comprise the following steps：
Step 1: by the rhizoma atractylodis of 3-5 parts by weight, the banksia rose of 1-2 parts by weight, the coptis of 5-8 parts by weight, 3-6 parts by weight Cortex Phellodendri,
The Rhizoma Atractylodis Macrocephalae of the dried orange peel of 5-10 parts by weight, the radix glycyrrhizae of 3-5 parts by weight and 2-4 parts by weight mixes after crushing respectively, obtains mixed-powder；
Step 2: after mixed-powder is placed in the water of 100-200 parts by weight, the cellulose of 0.1-1 parts by weight is added into water
The pectase of enzyme and 0.1-1 parts by weight, digests 2-3h at 40-50 DEG C, obtains enzymolysis liquid, backward enzymolysis liquid in add sugarcane
Sugar, the concentration for making sucrose is 0.4-0.6 g/mL, and cell is shunk, and after reaching balance, quickly adds water, makes sucrose in enzymolysis liquid
Concentration be 0.05-0.1 g/mL, be warming up to 90-100 DEG C afterwards, after destroy the enzyme treatment 30-60min, filtering, obtains the first filtrate
With the first filter residue；
Step 3: after the first filter residue is sealed by fermentation 5-10 days at 20-30 DEG C, filtering, obtains the second filtrate and the second filter residue；
Step 4: by the wood vinegar of 0.5-1 parts by weight, the lysozyme of 0.01-0.1 parts by weight, the chitosan of 1-2 parts by weight,
One filtrate and the mixing of the second filtrate, obtain mixed liquor.
3. prevent the application method of the compound formulation of Penaeus Vannmei white excrement disease, it is characterised in that including：
Step 1), by after the second filter residue and drying, carry out ultramicro grinding, mixed by per kilogram feed and take the second filter residues of 0.5-1g, in shrimp
Eat food the vigorous time, once a day, be used in conjunction 5-7 days；
Step 2), the 1-2h after shrimp eats food vigorous time, by the 150-180g/ mu meters mixed liquors unreasonable like a shrew into pond, even spread
5-7 days.
4. the application method of the compound formulation of prevention Penaeus Vannmei white excrement disease as claimed in claim 3, it is characterised in that institute
State step 2) in, using multiple flushers into pond mixed liquor unreasonable like a shrew；
The flusher includes：
Housing, it is cylindrical, and is vertically set, and feed inlet is provided with the top of the housing, and bottom interval is set
It is equipped with multiple discharge holes, at least two pairs of otic placodes, each pair otic placode is arranged at intervals with along its axis direction on the lateral wall of the housing
Set relative to the axisymmetrical of housing；
Partition plate, it is circular ring shape, and is arranged horizontally in the housing, and the outside diameter of partition plate is equal with the internal diameter of housing,
The outer ring of partition plate and the madial wall of housing are fixed and are seamlessly connected；
Guide cylinder, it is the cylinder bodily form, and is coaxially disposed with the housing, the top of the guide cylinder and the top of the housing
Be fixedly connected, the bottom of the guide cylinder passes through the middle part of the partition plate, and extends to the lower section of the partition plate, and with housing
Bottom does not contact, and multiple through holes are arranged at intervals with the side wall of the guide cylinder, and the multiple through hole is located at the top of the housing
Between portion and the partition plate；
Sleeve, it is the cylinder bodily form, and is set on the guide cylinder, the outside diameter phase of the internal diameter of the sleeve and the guide cylinder
Deng the outside diameter of the sleeve is equal with the internal diameter of the partition plate；
Power set, itself and the sleeve connection, for driving the sleeve to move up and down；
The step 2) in, use the specific method of multiple flushers from mixed liquor unreasonable like a shrew to pond for：
Multiple flushers are set at equal intervals along its width on pond, one is worn in the otic placode of each flusher
Steel wire rope, make steel wire rope along pond length direction extend, sleeve is sealed part or all of through hole, afterwards by feed inlet to
Mixed liquor is imported in housing, flusher is at the uniform velocity moved along the length direction in pond, while power set average rate pushes down on
Sleeve, mixed liquor after each discharge hole by being sprayed in pond.
5. the application method of the compound formulation of prevention Penaeus Vannmei white excrement disease as claimed in claim 4, it is characterised in that institute
The length for stating sleeve is more than the top of the housing and the distance of the partition room.
6. the application method of the compound formulation of prevention Penaeus Vannmei white excrement disease as claimed in claim 4, it is characterised in that institute
The center for stating the bottom of housing tilts gradually downward towards edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711308737.XA CN107998381B (en) | 2017-12-11 | 2017-12-11 | Compound preparation for preventing and treating white feces disease of penaeus vannamei boone and preparation and use methods thereof |
Applications Claiming Priority (1)
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| CN109452497A (en) * | 2018-12-29 | 2019-03-12 | 盐城工学院 | A kind of additive and the preparation method and application thereof preventing South America prawn white excrement disease |
| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
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| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
| CN115212276B (en) * | 2022-07-14 | 2023-11-21 | 集美大学 | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab |
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| CN107998381B (en) | 2021-09-07 |
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