CN107988325A - 虾肝肠胞虫(ehp)的raa恒温荧光检测方法及试剂 - Google Patents
虾肝肠胞虫(ehp)的raa恒温荧光检测方法及试剂 Download PDFInfo
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- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
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Abstract
Description
组别\结果 | CT值 | 荧光强度 |
第一组 | 14.21 | 330,000 |
第二组 | 17.97 | 170,000 |
第三组 | 15.47 | 380,000 |
第四组 | 12.93 | 560,000 |
RAA反应体系组分 | 体积(μL) |
A Buffer | 12.5μL |
B Buffer | 2.5μL |
引物混合液 | 4μL |
特异性荧光探针 | 0.6μL |
DNA模板 | 2μL |
ddH2O | 28.4μL |
总体积 | 50μL |
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Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109337990A (zh) * | 2018-11-13 | 2019-02-15 | 天津市水生动物疫病预防控制中心 | 一种虾肝肠胞虫可视化快速检测试剂盒 |
CN109735642A (zh) * | 2019-03-07 | 2019-05-10 | 江苏省血吸虫病防治研究所 | 一种raa荧光法检测蓝氏贾第鞭毛虫的引物、探针及检测方法 |
CN111485017A (zh) * | 2019-01-29 | 2020-08-04 | 广东美格基因科技有限公司 | 用于检测对虾虾肝肠孢虫的荧光定量pcr方法及相应的试剂盒 |
CN111635953A (zh) * | 2020-06-10 | 2020-09-08 | 广西壮族自治区水产科学研究院 | 基于TaqMan-MGB探针的虾肝肠胞虫荧光定量PCR检测引物组和试剂盒 |
CN113278718A (zh) * | 2021-06-09 | 2021-08-20 | 湛江海关技术中心 | 一种用于检测南美白对虾肝肠胞虫的引物对、扩增试剂、扩增试剂盒、检测方法和应用 |
CN113430274A (zh) * | 2021-06-11 | 2021-09-24 | 中山大学 | 一种检测肝肠胞虫的rpa引物、探针、试剂盒及方法 |
CN113913543A (zh) * | 2021-10-28 | 2022-01-11 | 天津市动物疫病预防控制中心 | Ehp实时荧光定量pcr检测引物探针组合、试剂盒及方法 |
CN114752695A (zh) * | 2022-04-13 | 2022-07-15 | 隆科生物(青岛)有限公司 | 一种对虾虾肝肠孢虫检测的荧光检测试剂 |
CN115852006A (zh) * | 2022-12-21 | 2023-03-28 | 中国海洋大学三亚海洋研究院 | 基于era技术的对虾肝肠胞虫快速检测方法及引物与探针组合 |
KR20230051991A (ko) | 2021-10-12 | 2023-04-19 | 한국생명공학연구원 | 양식 흰다리새우의 급성간췌장괴사증 및 새우미포자충의 동시 진단용 프라이머 세트 및 이의 용도 |
TWI817777B (zh) * | 2018-10-05 | 2023-10-01 | 福又達生物科技股份有限公司 | 用於偵測蝦微孢子蟲(Enterocytozoon hepatopenaei)之寡核苷酸對及探針 |
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2017
- 2017-11-10 CN CN201711106192.4A patent/CN107988325B/zh active Active
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XIAOMING XIA等: "Rapid detection of shrimp white spot syndrome virus by real time,isothermal recombinase polymerase amplification assay", 《PLOS ONE》 * |
刘珍: "虾肝肠胞虫实时荧光定量PCR检测技术的建立及其与对虾生长相关性的研究", 《中国优秀硕士学位论文全文数据库(农业科技辑)》 * |
刘雅梅: "虾肝肠胞虫Taqman荧光定量PCR检测技术的建立及其与对虾生长相关性的研究", 《中国优秀硕士学位论文全文数据库(农业科技辑)》 * |
骆云慧: "虾肝肠胞虫TaqMan实时荧光定量PCR检测方法的建立及应用", 《中国收益科学》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI817777B (zh) * | 2018-10-05 | 2023-10-01 | 福又達生物科技股份有限公司 | 用於偵測蝦微孢子蟲(Enterocytozoon hepatopenaei)之寡核苷酸對及探針 |
CN109337990A (zh) * | 2018-11-13 | 2019-02-15 | 天津市水生动物疫病预防控制中心 | 一种虾肝肠胞虫可视化快速检测试剂盒 |
CN111485017A (zh) * | 2019-01-29 | 2020-08-04 | 广东美格基因科技有限公司 | 用于检测对虾虾肝肠孢虫的荧光定量pcr方法及相应的试剂盒 |
CN109735642A (zh) * | 2019-03-07 | 2019-05-10 | 江苏省血吸虫病防治研究所 | 一种raa荧光法检测蓝氏贾第鞭毛虫的引物、探针及检测方法 |
CN111635953A (zh) * | 2020-06-10 | 2020-09-08 | 广西壮族自治区水产科学研究院 | 基于TaqMan-MGB探针的虾肝肠胞虫荧光定量PCR检测引物组和试剂盒 |
CN113278718A (zh) * | 2021-06-09 | 2021-08-20 | 湛江海关技术中心 | 一种用于检测南美白对虾肝肠胞虫的引物对、扩增试剂、扩增试剂盒、检测方法和应用 |
CN113430274A (zh) * | 2021-06-11 | 2021-09-24 | 中山大学 | 一种检测肝肠胞虫的rpa引物、探针、试剂盒及方法 |
KR20230051991A (ko) | 2021-10-12 | 2023-04-19 | 한국생명공학연구원 | 양식 흰다리새우의 급성간췌장괴사증 및 새우미포자충의 동시 진단용 프라이머 세트 및 이의 용도 |
CN113913543A (zh) * | 2021-10-28 | 2022-01-11 | 天津市动物疫病预防控制中心 | Ehp实时荧光定量pcr检测引物探针组合、试剂盒及方法 |
CN113913543B (zh) * | 2021-10-28 | 2023-09-22 | 天津市动物疫病预防控制中心 | Ehp实时荧光定量pcr检测引物探针组合、试剂盒及方法 |
CN114752695A (zh) * | 2022-04-13 | 2022-07-15 | 隆科生物(青岛)有限公司 | 一种对虾虾肝肠孢虫检测的荧光检测试剂 |
CN115852006A (zh) * | 2022-12-21 | 2023-03-28 | 中国海洋大学三亚海洋研究院 | 基于era技术的对虾肝肠胞虫快速检测方法及引物与探针组合 |
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Inventor after: Cheng Qi Inventor after: Shen Hong Inventor after: Zheng Xiaoye Inventor after: Zheng Tianlun Inventor after: Shen Weiliang Inventor after: Lv Wenhao Inventor after: Qian Dong Inventor after: Huang Zhenju Inventor after: Zhang Jianxun Inventor after: Xiao Wen Inventor after: Yu Guojun Inventor after: Tao Zhiyong Inventor after: Xu Jinyu Inventor after: Huo Shengnan Inventor before: Cheng Qi Inventor before: Shen Hong Inventor before: Zheng Xiaoye Inventor before: Zheng Tianlun Inventor before: Shen Weiliang Inventor before: Lv Wenhao Inventor before: Qian Dong Inventor before: Huang Zhenju Inventor before: Zhang Jianxun Inventor before: Xiao Wen Inventor before: Yu Guojun Inventor before: Tao Zhiyong Inventor before: Xu Jinyu Inventor before: Huo Shengnan |
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