CN107828913A - 对虾白斑综合症(wssv)的raa恒温荧光检测方法及试剂盒 - Google Patents
对虾白斑综合症(wssv)的raa恒温荧光检测方法及试剂盒 Download PDFInfo
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- CN107828913A CN107828913A CN201711107278.9A CN201711107278A CN107828913A CN 107828913 A CN107828913 A CN 107828913A CN 201711107278 A CN201711107278 A CN 201711107278A CN 107828913 A CN107828913 A CN 107828913A
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- wssv
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
Description
组别\结果 | CT值 | 荧光强度 |
第一组 | 7.23 | 270,000 |
第二组 | 9.11 | 310,000 |
第三组 | 9.56 | 330,000 |
第四组 | 5.02 | 430,000 |
RAA反应体系组分 | 体积(μL) |
A Buffer | 12.5μL |
B Buffer | 2.5μL |
引物混合液 | 4μL |
特异性荧光探针 | 0.6μL |
DNA模板 | 2μL |
ddH2O | 28.4μL |
总体积 | 50μL |
Claims (8)
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CN201711107278.9A CN107828913A (zh) | 2017-11-10 | 2017-11-10 | 对虾白斑综合症(wssv)的raa恒温荧光检测方法及试剂盒 |
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CN201711107278.9A CN107828913A (zh) | 2017-11-10 | 2017-11-10 | 对虾白斑综合症(wssv)的raa恒温荧光检测方法及试剂盒 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109385487A (zh) * | 2018-09-11 | 2019-02-26 | 浙江省食品药品检验研究院 | 贵细中草药西洋参的重组酶介导扩增恒温检测方法及试剂盒 |
CN110592240A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 哈维氏弧菌(vhv)的raa恒温荧光检测方法及试剂 |
CN110592275A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 鳜鱼传染性脾肾坏死病毒(isknv)的raa恒温荧光检测方法及试剂 |
CN110592267A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 鲤春病毒血症病毒(svcv)的raa恒温荧光检测方法及试剂 |
CN113444825A (zh) * | 2021-08-30 | 2021-09-28 | 至善时代智能科技(北京)有限公司 | 一种属水平鉴定环境细菌的引物组、试剂盒及恒温扩增方法 |
CN115786593A (zh) * | 2022-11-25 | 2023-03-14 | 湛江海关技术中心 | 一种用于对虾白斑综合征病毒检测的引物探针组合及试剂盒 |
CN117701777A (zh) * | 2024-01-22 | 2024-03-15 | 烟台海关技术中心 | WSSV检测引物、crRNA、CRISPR组合物、试剂盒及应用 |
Citations (2)
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WO2002029096A2 (fr) * | 2000-10-05 | 2002-04-11 | Skuld-Tech S.A.R.L. | Methode rapide de detection d'organismes dans un echantillon |
CN103088156A (zh) * | 2012-12-26 | 2013-05-08 | 杭州三合创新科技有限公司 | 白斑综合征病毒检测试剂盒及检测方法 |
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2017
- 2017-11-10 CN CN201711107278.9A patent/CN107828913A/zh active Pending
Patent Citations (2)
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WO2002029096A2 (fr) * | 2000-10-05 | 2002-04-11 | Skuld-Tech S.A.R.L. | Methode rapide de detection d'organismes dans un echantillon |
CN103088156A (zh) * | 2012-12-26 | 2013-05-08 | 杭州三合创新科技有限公司 | 白斑综合征病毒检测试剂盒及检测方法 |
Non-Patent Citations (4)
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XIAOMING XIA等: "Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay", 《PLOS ONE》 * |
YANG, F. 等: "White spot syndrome virus,complete genome", 《GENBANK DATABASE》 * |
张正阳 等: "白斑综合征病毒(WSSV)基因VP28原核表达与检测", 《上海海洋大学学报》 * |
阎隆飞 等: "《分子生物学》", 30 September 1993, 北京农业大学出版社 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110592240A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 哈维氏弧菌(vhv)的raa恒温荧光检测方法及试剂 |
CN110592275A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 鳜鱼传染性脾肾坏死病毒(isknv)的raa恒温荧光检测方法及试剂 |
CN110592267A (zh) * | 2018-06-13 | 2019-12-20 | 杭州众测生物科技有限公司 | 鲤春病毒血症病毒(svcv)的raa恒温荧光检测方法及试剂 |
CN109385487A (zh) * | 2018-09-11 | 2019-02-26 | 浙江省食品药品检验研究院 | 贵细中草药西洋参的重组酶介导扩增恒温检测方法及试剂盒 |
CN109385487B (zh) * | 2018-09-11 | 2022-04-01 | 浙江省食品药品检验研究院 | 贵细中草药西洋参的重组酶介导扩增恒温检测方法及试剂盒 |
CN113444825A (zh) * | 2021-08-30 | 2021-09-28 | 至善时代智能科技(北京)有限公司 | 一种属水平鉴定环境细菌的引物组、试剂盒及恒温扩增方法 |
CN115786593A (zh) * | 2022-11-25 | 2023-03-14 | 湛江海关技术中心 | 一种用于对虾白斑综合征病毒检测的引物探针组合及试剂盒 |
CN117701777A (zh) * | 2024-01-22 | 2024-03-15 | 烟台海关技术中心 | WSSV检测引物、crRNA、CRISPR组合物、试剂盒及应用 |
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Inventor after: Cheng Qi Inventor after: Shen Hong Inventor after: Zheng Xiaoye Inventor after: Zheng Tianlun Inventor after: Shen Weiliang Inventor after: Lv Wenhao Inventor after: Qian Dong Inventor after: Huang Zhenju Inventor after: Zhang Jianxun Inventor after: Xiao Wen Inventor after: Yu Guojun Inventor after: Tao Zhiyong Inventor after: Xu Jinyu Inventor after: Huo Shengnan Inventor before: Cheng Qi Inventor before: Shen Hong Inventor before: Zheng Xiaoye Inventor before: Zheng Tianlun Inventor before: Shen Weiliang Inventor before: Lv Wenhao Inventor before: Qian Dong Inventor before: Huang Zhenju Inventor before: Zhang Jianxun Inventor before: Xiao Wen Inventor before: Yu Guojun Inventor before: Tao Zhiyong Inventor before: Xu Jinyu Inventor before: Huo Shengnan |
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Application publication date: 20180323 |