CN107890486A

CN107890486A - A kind of Chinese medicine external anti-inflammation formulation and its preparation method and application

Info

Publication number: CN107890486A
Application number: CN201711258828.7A
Authority: CN
Inventors: 党岩; 梁剑平; 马志宏; 张莉敏; 常攀峰; 孙春香; 苟想珍
Original assignee: GANSU INSTITUTE OF ANIMAL HUSHANDRY VETERINARY MEDICINE
Current assignee: GANSU INSTITUTE OF ANIMAL HUSHANDRY VETERINARY MEDICINE
Priority date: 2017-12-04
Filing date: 2017-12-04
Publication date: 2018-04-10
Anticipated expiration: 2037-12-04
Also published as: CN107890486B

Abstract

The invention discloses a kind of Chinese medicine external anti-inflammation formulation and its preparation method and application, belong to field of veterinary, to solve the problems, such as that existing external preparation has that effective ingredient content is low, therapeutic effect is unstable, production cost is high.Wherein slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation is made from the following raw materials in parts by weight, 13 parts of slenderstalk dicranostigma herb preparation, 13 parts of honeysuckle Chinese traditional compound medicine and auxiliary material.The Chinese traditional compound medicines such as slenderstalk dicranostigma herb HCl preparation and honeysuckle are combined by the present invention, there is good synergy therebetween, there is good therapeutic effect to animal oral cavity skin ulcer, inflammation and wound, and effective percentage is 100%, and cure rate is between 85.7~100%.

Description

A kind of Chinese medicine external anti-inflammation formulation and its preparation method and application

Technical field

The invention belongs to field of veterinary, and in particular to a kind of Chinese medicine external anti-inflammation formulation and its preparation method and application.

Background technology

At present under the background of the green strategy implementation in China, social extensive concern food security, nuisanceless production is called Product and safe animal products production, it is particularly applied to food-borne animal drug and is rapidly converting to low toxicity, noresidue, efficient etc. hair Exhibition.

Animal oral cavity scytitis, ulcer and wound refer to animal mound as caused by Medicine and Surgery disease and communicable disease Rash, inflammation, ulcer, bleeding, suppuration and exterior trauma infection, are a variety of sex pheromones of the external skin as caused by the various causes of disease Caused by infection.These disease some are a hair in animal population, whole to domestic animals and fowls such as purulent inflammation and trauma infection contamination Body Influence of production is little, but some communicable diseases are then mass-sendings, such as sheep contagiousness dermatitis impetiginosa, incidence of disease average out to 75%, the incidence of disease of lamb is up to more than 90%, and what fatal rate had can reach 15% or so, and great shadow is produced to Animal husbandry production Ring；Meanwhile be zoonosis again as this kind of disease of sheep contagiousness dermatitis impetiginosa, people, cat, camel, musk-ox, reinder, Antelope, sea dog and dog etc. can also infect this disease, also have important influence to public health and environmental protection.Animal health degree is not Only it is related to the quality of life of people, and is related to the sanitarian safety of society.Although modern medicine, materia medica and molecule The development of biology, the preventing and treating to animal oral cavity scytitis, ulcer and wound provide multiple therapy methods, but in face of complexity Various animal oral cavity scytitis, ulcer and wound can not still solve in many aspects.

Although the development of modern medicine, materia medica and molecular biology, the preventing and treating to animal skin inflammation, ulcer and wound Provide a variety of medicines and method, but treat animal skin inflammation, ulcer and wound mainly with the stronger chemical drug of toxicity, anti- Based on rhzomorph, strong acid and strong base, Strong oxdiative reducing agent and animal nucleic acid protein denaturant.These chemical drugs are blindly used for a long time, are resisted Rhzomorph, strong acid and strong base, Strong oxdiative reducing agent and animal nucleic acid protein denaturant, the natural environment for making us survive are seriously polluted； Pathogenic microorganism is growing to these Drug-resistant strains；Damage of these medicines to animal skin normal tissue cell is serious, The reparation and growth of unfavorable animal skin tissue's cell.

Slenderstalk dicranostigma herb (Dicranostigma leptopodum(Maxim) Fedde, DLF), it is Papaveraceae convex combination algorithm two Year life or herbaceos perennial, are mainly distributed on the ground such as Gansu, Shaanxi.Since last century the eighties, China's many is ground Pharmacological action of the person of studying carefully to the composition and slenderstalk dicranostigma herb preparation of slenderstalk dicranostigma herb is studied, extracted from slenderstalk dicranostigma herb slenderstalk dicranostigma herb alkali, The multiple compounds such as ICD, corydaline, Biflorine, allocryptopine, sinoacutine.Research shows that slenderstalk dicranostigma herb is to tuberculosis Disease has good healing effect, cures curative effect more than 79.5%.

Although present slenderstalk dicranostigma herb external preparation is effective to diseases such as treatment animal skin inflammation, ulcer and wounds, slenderstalk dicranostigma herb External preparation has that effective ingredient content is low, therapeutic effect is unstable, production cost is high, manufacturing process is relative complex and faces Bed treatment five big shortcoming of operating difficulties.

The effective ingredient of Chinese herbal medicine slenderstalk dicranostigma herb is alkaloid, and its dissolving feature is other in addition to small molecule alkaloid The slightly soluble in water and Fat-soluble alkaloids have preferable dissolubility in the organic solvent such as ethanol and chloroform but the slightly soluble in water, The paste or pulvis that slenderstalk dicranostigma herb external preparation final products are water or alcoholic solution preparation and add adjuvant to be formed on this basis.Therefore The preparation method of slenderstalk dicranostigma herb preparation is mainly extracted with organic solvent at present, aqueous solvent extraction and various chromatography methods based on, it is auxiliary it The methods of dissolving and distill with supersonic oscillations.Zhao Qiang etc. preparation method be by slenderstalk dicranostigma herb powder at room temperature, it is molten with ethanol It is liquid-tight to close soak extraction, supersonic oscillations dissolving, ethanol evaporation, slenderstalk dicranostigma herb ethanol concentrated extract is made；Wang Tingpu etc. system Preparation Method is to extract slenderstalk dicranostigma herb herb by water, and through ethanol precipitation, concentrates and obtain slenderstalk dicranostigma herb preparation by lyophilized；Yin Jianhua Preparation method with beam sword equality is that slenderstalk dicranostigma herb powder is first passed through into water extraction effective ingredient, and medicine is extracted with ethanol after concentration Thing active ingredient, last ethanol evaporation obtain slenderstalk dicranostigma herb water soluble preparation.

Antimicrobial, anti-inflammatory in Chinese herbal medicine slenderstalk dicranostigma herb and adjust the active ingredient of animal body immunity function and recognize altogether at present Mainly alkaloid, the preparation method of slenderstalk dicranostigma herb preparation is mainly extracted with organic solvent at present, aqueous solvent extraction and various chromatographies Based on method, the methods of being aided with supersonic oscillations dissolving and distill；And the purpose due to user and purposes are Bu Tong respectively ground Study carefully that unit preparation method is different, the active ingredient and standard of preparation are also different.

The content of the invention

It is existing outer to solve it is an object of the invention to provide a kind of simplicity, economically and efficiently Chinese medicine external anti-inflammation formulation The problem of effective ingredient content is low, therapeutic effect is unstable, production cost is high be present with preparation.

It is a further object to provide a kind of preparation method of Chinese medicine external anti-inflammation formulation, to solve the existing favus of the scalp The problem of flower external preparation manufacturing process is relative complex.

It is also another object of the present invention to provide a kind of application of Chinese medicine external anti-inflammation formulation.

Technical solution of the present invention is as follows：A kind of Chinese medicine external anti-inflammation formulation, it is honeysuckle Chinese traditional compound medicine, by following The raw material of parts by weight is made：Honeysuckle 60-80 parts, rhizoma atractylodis 30-60 parts, capsule of weeping forsythia 60-80 parts, radix scutellariae 60-80 parts, golden cypress 60-80 Part, Radix Isatidis 60-80 parts, cordate houttuynia 30-60 parts and radix glycyrrhizae 30-60 parts.

As another embodiment of the invention, a kind of Chinese medicine external anti-inflammation formulation is that slenderstalk dicranostigma herb preparation is answered with honeysuckle The slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation of square Chinese medicine preparation, be using water miscible alkaloid salt hydrochlorate as main component, it is auxiliary With the dissolubility and effect of phenmethylol, glycerine and ethanol increase effective ingredient.It is made from the following raw materials in parts by weight, the favus of the scalp Flower preparation 1-3 parts, honeysuckle Chinese traditional compound medicine 1-3 parts and auxiliary material, auxiliary material are ethanol 1-2 parts or glycerine or medical starch 1-2 Part.

The preparation method of honeysuckle Chinese traditional compound medicine, comprises the following steps in a kind of above-mentioned Chinese medicine external anti-inflammation formulation：

（1）Honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia and radix glycyrrhizae are weighed according to formula ratio, in addition to rhizoma atractylodis, Other medicines are poured into porcelain pot, and water 1600-2000ml, absolute ethyl alcohol 400-500ml, phenmethylol 5-15ml are added in pot With glycerine 5-15ml, pH to 5.0-6.0, sealing immersion are adjusted with hydrochloric acid after stirring；Stirred once every 30-60 minutes；It is grey Art seals immersion with 100-200ml alcohol in another container；Drug immersion 12-24h；

（2）After drug immersion, rhizoma atractylodis immersion is incorporated into pot with other drugs soak and stirred, 1.5- is boiled in heating 2h, after fluid temperature is down to 50-60 C °, the decoction 0-4 C ° filtered in pot are saved backup and are abandoned precipitation；

（3）Repeat the above steps（1）With（2）3-5 times；

（4）The dregs of a decoction are abandoned, merges above-mentioned each gained decoction, medicine liquid heating evaporation is carried out being concentrated into 250-500ml, when decoction temperature After degree is down to room temperature, product is produced after filtration high pressure sterilizing.

Further, said preparation is electuary or spray, honeysuckle Chinese traditional compound medicine 4-8 parts, adds 1-2 part ethanol, stirs Mixing is mixed to form.

Further, said preparation is pulvis, and honeysuckle Chinese traditional compound medicine 2-4 parts, medical starch 1-2 parts, the two is in room Temperature, which mixes, to be air-dried, grind.

Further, said preparation is paint, honeysuckle Chinese traditional compound medicine 4-8 parts, is heated and flashes to paste；With doctor With glycerine 0.5-1 parts in room temperature mixing.

A kind of preparation method of above-mentioned Chinese medicine external anti-inflammation formulation, slenderstalk dicranostigma herb preparation therein are prepared by following steps,

(1) slenderstalk dicranostigma herb dry powder 500-1000 parts are weighed, loads in multilayer gauze bag, is placed in porcelain pot, two is added and steams water 1600-2000ml, absolute ethyl alcohol 400-500ml, pH to 2.0-3.0, sealing immersion 12-24h are adjusted with hydrochloric acid after stirring;

(2) by the medical liquid squeezing in husky cloth medicine bag into pot, filtering liquid medicine simultaneously abandons precipitation；By the decoction after filtering and medicine bag one With being placed in pot, 1.5-2h is boiled in heating, and after fluid temperature is down to 20-25 C °, the decoction in husky cloth medicine bag is screwed onto into pot In pot, filter the decoction in pot and abandon precipitation；Then the pH value of decoction is adjusted to 10.0-11.0 with NaOH, filtering precipitation is standby With；After medicine liquid heating is boiled into 1.5-2h, multiply heat filtering decoction and abandon precipitation, decoction adjusts pH to 2.0-3.0 with hydrochloric acid, and filtering is sunk Form sediment standby；Decoction is poured out 0-4 C ° and saved backup；

（3）Medicine bag is placed in pot again, repeat step（1）And step（2）In immersion, boil, adjust pH value step, so Repeat 3-5 times；

（4）Abandon medicine bag, merge each time obtained by decoction, add 50-150ml phenmethylols and 50-150ml glycerine, with NaOH by decoction pH Value is adjusted to 5.0-6.0, and medicine liquid heating evaporation is carried out being concentrated into 500-1000ml, and above-mentioned after fluid temperature is down to room temperature The standby drug precipitation of step is mixed into suspension, and product is produced after autoclaving.

Further, the slenderstalk dicranostigma herb preparation is electuary or spray, slenderstalk dicranostigma herb preparation 4-8 parts, adds 1-2 part ethanol, stirs Mixing is mixed to form.

A kind of preparation method of above-mentioned Chinese medicine external anti-inflammation formulation, slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation therein Preparation process is as follows：

（1）It will be mixed after slenderstalk dicranostigma herb preparation, the dissolving of honeysuckle Chinese traditional compound medicine, be heated to 75-70 DEG C；

（2）When equitemperature is down to room temperature, auxiliary material is added, stirs and evenly mixs into slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation.

A kind of application of the above-mentioned Chinese medicine external anti-inflammation formulation in treatment animal oral cavity skin ulcer, inflammation and wound.

The drug ingedient that the present invention is easily metabolized with the distinctive low toxicity of Chinese traditional herbs solves the ring of animal externally applied anti-inflammation medicine Border pollution problem；Solves the micro- life of cause of disease with the distinctive antimicrobial composition of Chinese traditional herbs and the diversity and concertedness of effect The Drug-resistant sex chromosome mosaicism of thing；With the security of the distinctive effective ingredient of Chinese traditional herbs and to damaging tissue outside animal body Immunoloregulation function solve the problems, such as the quick repairing growth of animal skin injury tissue cell.

Chinese medicine external anti-inflammation formulation of the present invention, the first embodiment is honeysuckle Chinese traditional compound medicine, by Chinese herbal medicine monarch The compound principle that minister assistant makes, honeysuckle Chinese traditional compound medicine is monarch with clearing heat and detoxicating antimicrobial class medicine honeysuckle, with heat-clearing solution The antimicrobial class medicine capsule of weeping forsythia of poison, radix scutellariae are the increase of removing toxic substances anti-microbial effect scope and action effect enhancing that minister makes compound；With Clearing heat and detoxicating antimicrobial class medicine golden cypress, cordate houttuynia are that minister increases the anti-inflammatory antiulcer of compound and anti-microbial effect effect By force；Strengthen the antivirus action of compound by minister of clearing heat and detoxicating antiviral agent Radix Isatidis；Using dampness removing anti-inflammatory type medicine rhizoma atractylodis as assistant, Dampness removing acid suppression suppression ulcer and the protection mucous membrane effect of enhancing compound are cooperateed with minister golden cypress etc., using warm nature antipyretic and antidotal type medicine radix glycyrrhizae to make Mediation the monarch and his subjects' adjutant, makes the anti-inflammatory rehabilitative action effect of compound strengthen；Using ethanol, medical starch and glycerine as formulation adjutant, assign In preparation difference kenel, sick position and protection mucous membrane are reached beneficial to effective ingredient.

Chinese medicine external anti-inflammation formulation of the present invention, second of embodiment are slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation, with Eight kinds of Chinese herbal medicines are raw material, and it is formulation adjutant to be aided with ethanol, medical starch or glycerine, and the slenderstalk dicranostigma herb herbal mixture external application of preparation disappears Scorching preparation, it can preferably treat animal skin inflammation, ulcer and wound.

Although slenderstalk dicranostigma herb externally applied anti-inflammation preparation and honeysuckle Chinese traditional compound medicine have good treatment animal skin inflammation, The effect of ulcer and wound, but it is inconsistent for the disease therapeuticing effect of same symptom caused by Different types of etiopathogenises, cause this As a result the reason for be for the single antimicrobial pedigree of anti-inflammation formulation of the disease of same clinical condition caused by Different types of etiopathogenises not One and relatively narrow.Using between slenderstalk dicranostigma herb externally applied anti-inflammation preparation and honeysuckle Chinese traditional compound medicine to animal skin inflammation, The mutual synergy of ulcer and wound, effect pedigree scope of the increase medicine to animal skin inflammation, ulcer and wound；Enhancing Medicine is to being the master for developing slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation to the stress efficacy of animal skin inflammation, ulcer and wound Want the first purpose.

By the compound principle of Chinese herbal medicine monarch, slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation resists micro- life with clearing heat and detoxicating Species medicine slenderstalk dicranostigma herb is monarch, with honeysuckle Chinese traditional compound medicine it is auxiliary it（With clearing heat and detoxicating antimicrobial class medicine honeysuckle, the capsule of weeping forsythia, Radix scutellariae is that minister makes the removing toxic substances anti-microbial effect scope of compound increase and action effect enhancing；With clearing heat and detoxicating antimicrobial class medicine Golden cypress, cordate houttuynia are anti-inflammatory antiulcer and the enhancing of anti-microbial effect effect that minister makes compound；With clearing heat and detoxicating antiviral agent Radix Isatidis is that minister strengthens the antivirus action of compound；It is that minister etc. cooperates with enhancing with golden cypress using dampness removing anti-inflammatory type medicine rhizoma atractylodis as assistant The dampness removing acid suppression suppression ulcer of compound and protection mucous membrane effect, using warm nature antipyretic and antidotal type medicine radix glycyrrhizae as mediation the monarch and his subjects' adjutant is made, make multiple The anti-inflammatory rehabilitative action effect enhancing of side）；Using ethanol, medical starch or glycerine as formulation adjutant, preparation difference kenel, profit are given Sick position and protection mucous membrane are reached in effective ingredient.

Honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, folium isatidis, Radix Isatidis, cordate houttuynia and radix glycyrrhizae are mostly in antipyretic and antidotal type Herbal medicine in these Chinese herbal medicines to contain organic acid, alkaloid, glycoside or glucoside, polysaccharide, flavones, sterol, volatile oil and micro member more Element etc., there is antimicrobial and anti-inflammatory, while have preferable synergy between these Chinese herbal medicines.These above-mentioned medium-height grass Medicine not only has good anti-microbial effect, and has the function of regulation animal body immune system so as to promoting animal body Wound healing.The easily decomposes metabolism of composition in Chinese herbal medicine, the green and public health to the mankind have important meaning.Examination Result is tested to show, honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia, radix glycyrrhizae and slenderstalk dicranostigma herb preparation share have it is good Well antimicrobial, anti-inflammatory and promotion wound healing synergy.I is engaged in the research work of the Chinese herbal medicines such as slenderstalk dicranostigma herb for many years, On the basis of slenderstalk dicranostigma herb electuary, injection, liniment and oral capsule is developed, compound slenderstalk dicranostigma herb preparation is developed, is faced with solving animal doctor Bed operating difficulties, makes treatment become time saving and energy saving on the basis of curative effect is ensured.

Antimicrobial, anti-inflammatory and immunoregulatory active ingredient in Chinese herbal medicine slenderstalk dicranostigma herb are sanguinarine, allocryptopine, wood The alkaloids such as blue alkali, cucoline.Sanguinarine in slenderstalk dicranostigma herb has the effect such as antibacterial, antitumor, anti-trypanosome.Result of study shows, Sanguinarine hydrosulphate has inhibitory action in 717 mg/L concentration to koff-Wal gram trichobacteria and acrothesium floccosum；It is right Staphylococcus aureus, candida albicans and the small spore mould of ulotrichy have antibacterial action, to the minimum suppression of staphylococcus aureus Concentration is 1.95 mg/L-3.9 mg/L；Sanguinarine is stronger to the activity of Aeromonas hydrophila, its minimum inhibitory concentration and minimum Antimicrobial concentration is respectively 12.5 mg/L and 25 mg/L.It is weaker to the activity of Vibrio harveyi, Vibrio anguillarum, aeromonas salmonicida；Blood Root alkali has anti-dimension worm effect, and the formation to trypanosoma bocagei and trypanosoma confusum blood flow has inhibitory action, ED₅₀＜ 10mmol/L;Its sulphur 1/40000 solution of hydrochlorate and nitrate is still 100% to the inhibitory action of Louis silver worm after 24h；Sanguinarine refers to fish Ring worm, apple Tetranychus urticae, oncomelania have killing action；Sanguinarine can make human leukaemia K562 dead, can resist cervical cancer cell Drug tolerance, have inhibitory action to ehrlich ascites tumor, suppress the growth and transfer of cervical cancer cell, p53- can be induced Liver cancer cells are had by mutant and p53-null clones cancerous cell line, human pancreatic cancer cell AsPC-1 and BxPC-3 apoptosis There is obvious lethal effect, in vitro test confirms there is very strong cytotoxic activity to Human Prostate Cancer Cells.It is white in the wrong in slenderstalk dicranostigma herb The red alkali of dish has antibacterial and the effect such as antitumor.Experiment results proved, Chelerythrine to cariogenic bacteria, actinobacillus actinomycetem comitans, Becoming streptobacillus has inhibitory action, has inhibitory action to the pathogen of a variety of melons and fruits and vegetables, Chelerythrine can induce human gastric cancer The apoptosis of BGC823 cells, mankind's uveal melanoma bacterial strain, the propagation of cervical cancer cell can be suppressed.In addition, Chelerythrine Still there is anti-inflammation.Magnoline in slenderstalk dicranostigma herb has cytotoxicity, antibacterial, anti-oxidant, anti-inflammatory to tumor cell line Deng effect.Chen Jiahui etc. determines the antibacterial action of the two using flat band method with β, the αisomer of blue alkali, experiment results proved, and β- Magnoline is 10 μ g/disc to hazel color mould UC-4376 minimal inhibitory concentrations, is 100 μ to Pyricularia oryzae minimal inhibitory concentration g/disc；α-magnoline is 5 μ g/disc to hazel color mould minimal inhibitory concentration.Allocryptopine in slenderstalk dicranostigma herb have it is antitumor, Antibacterial, desinsection etc. acts on.Allocryptopine has antineoplastic action, and suppress DNA topoisomerases is probably its antitumor machine System.Allocryptopine is inhibited to various bacteria, has killing to make to cabbage caterpillar, red flour beetle adult and Culex quinquefasciatus With.The immunological regulation of cucoline in slenderstalk dicranostigma herb and antiinflammatory action.Cucoline can suppress the mouse of LPS and PMA inductions in vivo Spleen lymphocyte proliferation, ConA, LPS and anti-CD can be suppressed in vitro₃Inducing mouse spleen lymphocyte proliferation, rat can be made Elevated CD₄/ CD₈Ratio reduces, and mouse spleen lymphocyte early apoptosis percentage can in vitro dramatically increased.Sinomenium acutum Alkali can suppress the synthesis of IL -2, shorten the formation cycle of the T lymphocytes of activation, improve the survival rate of high immune response individual, Suppress macrophage and T-lymphocyte.Mouse macrophage can be made to reduce prostaglandin E in experiment in vitro₂, leukotriene C₄'s Synthesize and suppress nitric oxide production generation.Cucoline has significant regressive effect to rat formaldehyde and pale blue property " arthritis ", Have to the initiative anaphylactic shock of mouse it is pre- prevent from acting on, have therapeutic action to adjuvant arthritis ratses.Other slenderstalk dicranostigma herb In pentacyclic triterpenoid also have good anti-inflammatory and antibacterial action.

Honeysuckle Chinese traditional compound medicine is made up of nine kinds of Chinese herbal medicines, have between each other good antimicrobial, anti-inflammatory and Wound repair.Chinese herbal medicine honeysuckle is Caprifoliaceae woodbine honeysuckle and congener dry flower or with just opening Flower, its effective ingredient Main Flavonoids class, triterpenes, organic acid etc. and volatile oil, honeysuckle have antibacterial and antivirus action： To staphylococcus aureus, hemolytic streptococcus, dysentery, typhoid fever, diplococcus meningitidis, Diplococcus pneumopniae, green pus and influenza Virus etc. has obvious inhibiting effect, if being shared with the capsule of weeping forsythia, antibacterial range can also be complementary；Honeysuckle has the immune function of enhancing： Honeysuckle can promote lymphocyte transformation, strengthen the phagocytic function of leucocyte.Honeysuckle decoction is diluted to 1：1280 concentration, still The phagocytic function of leucocyte can be promoted.Mouse peritoneal injection honeysuckle flower injection, also it is obviously promoted inflammatory cell phagocytic function Effect；Honeysuckle has the function of anti-inflammatory：Honeysuckle can promote the release of cortex hormone of aadrenaline, have obvious suppression to make to inflammation With.Flos Lonicerae extractive solution 0.25g/kg is injected intraperitoneally, Rat Carrageenan colloidality pin can be suppressed and swollen.Separately have been reported that honeysuckle flower injection 30-40g/kg can mitigate the swollen degree of egg white pin.It is injected intraperitoneally Flos Lonicerae extractive solution 8g/kg, 2 times/day, continuous 6 days, to rat Crotons oiliness granulation capsule, also play the role of it is obvious it is impervious go out and anti-proliferate.Capsule of weeping forsythia glycoside is mainization contained by the Chinese herbal medicine capsule of weeping forsythia Study point, mainly include forsythin and two kinds of compositions of Forsythoside.The capsule of weeping forsythia has resisting pathogenic microbes effect：The capsule of weeping forsythia is blue to a variety of leather Positive and negative bacteria has inhibitory action, its concentrated decoction to suppress typhoid bacillus, paratyphosum Bacterium, large intestine bar in vitro Bacterium, shigella dysenteriae, corynebacterium diphtheriae and comma bacillus, staphylococcus, streptococcus etc., the capsule of weeping forsythia bacteriostasis in vitro and honeysuckle It is substantially similar;It is the main component of antibacterial, honeysuckle in Yinqiao San to Salmonella, particularly typhoid bacillus and hemolytic chain The inhibitory action of coccus seemingly exceedes the capsule of weeping forsythia, and the suppression to shigella dysenteriae, staphylococcus aureus is then with the capsule of weeping forsythia like preferable;In The medicine capsule of weeping forsythia has antiinflammatory action：50% Forsythinol solution of extract 20ml/kg intraperitoneal injections, to rat crotons oiliness granulation Capsule have obviously anti-transudation and reduction inflammation stove wall of micrangium fragility effect, the capsule of weeping forsythia can promote inflammatory barrier formation, 300% 30~40g/kg of capsule of weeping forsythia parenteral solution intraperitoneal injections, swollen to rat egg white pin have obvious inhibiting effect;Also can promote pair The phagocytosis of mouse inflammatory cell.Containing baicalin, baicalein, Chinese a kind of reed mentioned in ancient books element, B- sitosterol, wogonoside, Huang in Chinese herbal medicine radix scutellariae New element of a kind of reed mentioned in ancient books etc., radix scutellariae has antibacterial action：In vitro test radix scutellariae is to shigella dysenteriae, typhoid bacillus, Pseudomonas aeruginosa, staphylococcus, molten Courageous and upright streptococcus, Diplococcus pneumopniae etc. have stronger antibacterial action；Radix scutellariae has antivirus action：Radix scutellariae is to influenza type A Viral PR3 has inhibitory action；Animal is found again（Mouse）After infecting influenza, radix scutellariae can have certain therapeutic action；Radix scutellariae has antimycotic Effect：In vitro test radix scutellariae decoction is to a variety of dermatophytes（Such as epidermophyton inguinale）There is different degrees of inhibitory action.In Herbal medicine golden cypress mainly has the antibacterial action of wide spectrum containing a variety of alkaloids, golden cypresses such as jamaicin, Palmatine, phellodendrines, to various dysentery The inhibitory action of Shigella is especially strong, has synergy with the antibacterial activity of the coptis, golden cypress；Have to tubercle bacillus, Leptospira Stronger suppression and killing action；There is significant inhibitory action to a variety of fungies, trichomonad；External application can be used for treating in golden cypress Sore swollen toxin, eczema itch；Golden cypress has anti-trichomonal effect, 10% concentration golden cypress decoction and trichomonad liquid 1：1 mixes culture, to vagina Trichmonad has inhibitory action；Golden cypress has antihepatitic activity, 6.25-100% golden cypress decoction in vitro tests, has to hepatitis B antigen Inhibitory action, phellodendrine have certain effect to chronic hepatitis；Golden cypress has an influence to immunologic function, 100% concentration golden cypress decoction, 0.3ml/ gavage, continuous 7 days, mice spleen plaque forming cells's number can be increased；Golden cypress has antiulcer action, phellodendron extract （Remove jamaicin）100mg/kg is subcutaneously injected, 100,1000mg/kg gavages or intracutaneous injection, to ethanol, aspirin or deep and remote The rat gastric ulcer that door ligation induces has inhibitory action.Chinese herbal medicine cordate houttuynia contains decanoyl acetaldehyde, capraldehyde, lauryl aldehyde and methyl nonyl ketone Deng composition, wherein decanoyl acetaldehyde has resisting pathogenic microbes activity, and cordate houttuynia has resisting pathogenic microbes effect, suppresses golden yellow Color staphylococcus, Bacillus influenzae, Diplococcus pneumopniae；Cordate houttuynia has enhancing immune system effect, improves chronic bronchitis patients The phagocytosis of white blood cell；Cordate houttuynia has antitumor action, improves the CAMP in cancer cell and suppresses ehrlich ascites cancer；Fish raw meat Grass can be used for treating pathopyretic ulcer, often be used together with heat-clearing and detoxifying drugs such as Chinese violet, honeysuckle, the capsules of weeping forsythia, oral administration and externally.In Herbal medicine Radix Isatidis is the root of cruciferae isatis and careless smalt, the root and rhizome of acanthaceous vegetable acanthaceous indigo.Radix Isatidis have compared with The antibacterial action of wide spectrum；Radix Isatidis has inhibitory action to multiple pathogenic microorganisms such as virus, Leptospira, fungies；Radix Isatidis It is usually used in treating the infection of the upper respiratory tract, sphagitis, herpes zoster, epidemic acute conjunctivitis, viral encephalitis, tonsillitis, prevalence The infectious diseases such as property parotitis, stomatitis, canker sore.Chinese herbal medicine rhizoma atractylodis are composite family [WTBX herbaceos perennials, grey There is bacteriostasis containing chemical composition, rhizoma atractylodis such as terpene, flavones, polyene alkynes and volatile oils in art：Atractylodes chinensis, which has, to be eliminated The effect of resistance shigella flexneri R plasmids, can reduce the generation of bacterial drug resistance.Rhizoma atractylodis are soaked using 95% ethanol 10 hours, Rhizoma atractylodis are taken out, is positioned ready on the floor of operating room of sterilization, lights, it is more empty than before sterilization after sterilization untill rhizoma atractylodis turn to ash Clump count significantly reduces in gas；Rhizoma atractylodis have antitumor action：Rhzoma Atractylodis Lanceae volatile oil, hinesol, sagittol l00mg/ml are in vitro There is inhibitory action to esophageal cancer cell, wherein hinesol acts on stronger：Rhizoma atractylodis have stronger antiulcer action：Experiment discovery, thatch Rhizoma atractylodis and Atractylis chinensis have stronger suppression to make to pyloric ligation ulcers ulcer, pylorus ligation-aspirin ulcer, stress ulcer With ulcer, the gastric juice amount of animal, total acidity, total digestion power and Gastric mucosal lesion can be significantly inhibited.Chinese herbal medicine radix glycyrrhizae is pulse family Herbaceos perennial.With a variety of medicinal efficacies, more than 100 kinds of flavone compound, 60 are isolated from radix glycyrrhizae both at home and abroad A variety of triterpene compounds and Coumarins, 18 kinds of amino acid, a variety of alkaloids, female hormone and a variety of organic acids etc..It is sweet Grass has resisting pathogenic microbes effect：Glycyrrhizin can directly destroy test tube inner virus cell, to varicella, herpes zoster virus There is inhibitory action.The antivirus action of glycyrrhizin except the direct effect to virion, with its inducement interferon, increase from Natural killer cell activity also has certain relation：Radix glycyrrhizae has antipyretic and antidote functions：Radix glycyrrhizae is warm-natured, with heat-clearing, antipyretic toxic action The disease such as treatment toxication and the swollen serious case of furuncle of pain has certain effect；Radix glycyrrhizae has coordinating the drug actions of a prescription effect：Radix glycyrrhizae plays the role of Slow, uses radix glycyrrhizae Hou, the meaning of natural You Dot Tone sums, also there is the effect of its term of validity of Yan Slow And La Long every Ge Drug Drug Slow sweet slightly once Fruit；Radix glycyrrhizae has anti-inflammatory and anti-allergy action：Enoxolone is to the granuloma induced by implantation of cotton pellets of big white mouse, formaldehyde oedema, tulase Element reaction, subcutaneous granulation capsule inflammation, there is inhibitory action.Radix glycyrrhizae acid amide, Sodium glycyrrhetinate can effectively influence subcutaneous granulation Cystic inflammation oozes out phase and proliferative phase, and its anti-inflammatory intensity is weaker than or close to cortisone；Radix glycyrrhizae has detoxication：Experiment card Bright radix glycyrrhizae and its several formulations have certain detoxication to multi-medicament poisoning, animal poisoning, bacterium poisoning etc., can alleviate Poisoning symptom, reduce the death rate of intoxicated animals.

Slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation is mainly by slenderstalk dicranostigma herb preparation and honeysuckle Chinese traditional compound medicine Effective ingredient collaboration prepare, ethanol major function be ancillary drug it is antimicrobial effect, increase fat-soluble medicine have The dissolubility of composition is imitated, increases the concentration of effective ingredient and medicine is accessible to lesions position.Slenderstalk dicranostigma herb herbal mixture Externally applied anti-inflammation preparation main purpose is to increase the antimicrobial and antiinflammatory action pedigree of spray, promotes wound healing synergy, Make clinical treatment animal oral cavity skin ulcer, inflammation and wound time saving and energy saving.It is demonstrated experimentally that honeysuckle Chinese traditional compound medicine, bald Sore spends externally applied anti-inflammation preparation and slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray to have very animal oral cavity skin ulcer, inflammation and wound Good therapeutic effect, effective percentage are 100%, and cure rate is between 85.7~100%.

The innovative point of the present invention is as follows：

1. the preparation method of slenderstalk dicranostigma herb preparation of the present invention is to make effective ingredient extract solution with acid solution, its principle is to use Effective ingredient alkaloid in Chinese herbal medicine slenderstalk dicranostigma herb is changed into alkaloid salt hydrochlorate by hydrochloric acid, greatly increases slenderstalk dicranostigma herb Chinese medicine Dissolubility of the thing active ingredient in extract solution, solve the problems, such as that slenderstalk dicranostigma herb preparation of traditional Chinese medicine active constituent content is low；

2. the unstable basic reason of slenderstalk dicranostigma herb preparation for treating effect is the antimicrobial scope of slenderstalk dicranostigma herb preparation（Pedigree）Compared with It is narrow.It is wider antimicrobial that experiment shows that the slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation developed of the present invention has than slenderstalk dicranostigma herb preparation Scope（Pedigree）Narrower and stronger anti-microbial effect, the present invention are unstable so as to solve slenderstalk dicranostigma herb preparation for treating effect Problem；

3. the slenderstalk dicranostigma herb formulation preparation method of the present invention is to replace ethanol extraction method with 20% ethanol, the production of preparation is reduced Cost；The slenderstalk dicranostigma herb formulation preparation method of the present invention is to mix extraction method with acid alcohol water, while instead of the extraction of slenderstalk dicranostigma herb water Two processes are followed the example of in method and alcohol extracting, and have evaporated ethanol in slenderstalk dicranostigma herb medicine boiling extraction process, have saved ethanol evaporation backflow Process, the preparation technology of slenderstalk dicranostigma herb preparation is simplified, solve the problems, such as that slenderstalk dicranostigma herb external preparation manufacturing process is relative complex；

4. due to the particularity for the treatment of animal external skin inflammation, ulcer and wound, such as safety for the treatment of staff, animal mouth The fraction medicines such as the treatment difficulty at the positions such as intracavitary portion, animal hoof are difficult to fixed, animal external skin inflammation ulcer and wound Group for the treatment of etc., therefore the present invention is according to the external skin inflammation of animal different parts and different situations, ulcer and outer Hinder disease, prepare the treatment for being used for animal external skin inflammation, ulcer and wound using different dosage forms, ensuring to treat curative effect On the basis of, simplify therapeutic process, save treatment time, it is ensured that treatment operating personnel's safety, so as to solve clinical treatment operation The problem of difficult.

Embodiment

Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments Method, it is conventional method unless otherwise specified.Test material used, is city unless otherwise specified in following embodiments Sell.

Embodiment 1- honeysuckle Chinese traditional compound medicines

Raw material forms：Honeysuckle 60g, rhizoma atractylodis 30g, capsule of weeping forsythia 60g, radix scutellariae 60g, golden cypress 60g, Radix Isatidis 60g, cordate houttuynia 30g and Radix glycyrrhizae 30g.

Embodiment 2- honeysuckle Chinese traditional compound medicines

Raw material forms：Honeysuckle 70g, rhizoma atractylodis 50g, capsule of weeping forsythia 70g, radix scutellariae 70g, golden cypress 70g, Radix Isatidis 70g, cordate houttuynia 50g and Radix glycyrrhizae 50g.

Embodiment 3- honeysuckle Chinese traditional compound medicines

Raw material forms：Honeysuckle 80g, rhizoma atractylodis 60g, capsule of weeping forsythia 80g, radix scutellariae 80, golden cypress 80g, Radix Isatidis 80g, cordate houttuynia 60g and sweet Careless 60g.

The preparation of embodiment 4- honeysuckle Chinese traditional compound medicines

（1）Accurately weighed with electronic balance according to formula ratio honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia and Radix glycyrrhizae.Except rhizoma atractylodis load in multiple multilayer gauze bags with the alcohol of 200ml 95% in addition to the sealing immersion of another container, other medicines are equal Pour into porcelain pot, two are added in pot and steams water 1600ml, absolute ethyl alcohol 400ml (analysis is pure), phenmethylol (analysis is pure) 10ml and glycerine (analysis is pure) 10ml, pH to 5.0, sealing immersion are adjusted after stirring with hydrochloric acid (analysis is pure).Every 30 minutes Stirring is once.So by drug immersion 12h.

（2）After drug immersion, rhizoma atractylodis medicine and decoction are incorporated into pot and stirred, 2h is boiled in heating, works as decoction Temperature is down to 60 after C °, and C ° of the decoction 4 filtered in pot saves backup and abandoned precipitation.

（3）Repeat the above steps（1）With（2）4 times；

（4）The dregs of a decoction are abandoned, merges 4 gained decoctions, medicine liquid heating evaporation is carried out being concentrated into 500ml, when fluid temperature is down to room Wen Hou, honeysuckle Chinese traditional compound medicine is produced after filtration high pressure sterilizing, 4C ° is put and saves backup.

The preparation of embodiment 5- honeysuckle Chinese traditional compound medicines

（1）Honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia and radix glycyrrhizae are weighed according to formula ratio, in addition to rhizoma atractylodis, Other medicines are poured into porcelain pot, and two are added in pot and steams water 2000ml, absolute ethyl alcohol 500ml, phenmethylol 15ml and glycerine 15ml, pH to 6.0, sealing immersion are adjusted with hydrochloric acid after stirring；Every stirring in 60 minutes once；Rhizoma atractylodis with 100 95% alcohol Immersion is sealed in another container；Drug immersion 24h；

（2）After drug immersion, rhizoma atractylodis medicine and decoction are incorporated into pot and stirred, 1.5h is boiled in heating, when decoction temperature Degree is down to 50 after C °, and C ° of the decoction 0 filtered in pot saves backup and abandoned precipitation；

（3）Repeat the above steps（1）With（2）3 times；

（4）The dregs of a decoction are abandoned, merges above-mentioned each gained decoction, medicine liquid heating evaporation is carried out being concentrated into 250ml, when fluid temperature drops To room temperature, product is produced after filtration high pressure sterilizing, C ° is set to 0 and saves backup.

The preparation of embodiment 6- honeysuckle Chinese traditional compound medicines

（1）Accurately weighed with electronic balance according to formula ratio honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia and Radix glycyrrhizae.Except rhizoma atractylodis load in multiple multilayer gauze bags with the alcohol of 200ml 95% in addition to the sealing immersion of another container, other medicines are equal Pour into porcelain pot, two are added in pot and steams water 1600ml, absolute ethyl alcohol 400ml (analysis is pure), phenmethylol (analysis is pure) 5ml and glycerine (analysis is pure) 5ml, pH to 5.0, sealing immersion are adjusted after stirring with hydrochloric acid (analysis is pure).Stirred every 30 minutes Mix once.So by drug immersion 12h.

（3）Repeat the above steps（1）With（2）5 times；

（4）The dregs of a decoction are abandoned, merges 5 gained decoctions, medicine liquid heating evaporation is carried out being concentrated into 500ml, when fluid temperature is down to room Wen Hou, honeysuckle Chinese traditional compound medicine is produced after filtration high pressure sterilizing, 4C ° is put and saves backup.

The preparation of embodiment 7- honeysuckles herbal mixture externally applied anti-inflammation electuary and spray

Precise honeysuckle Chinese traditional compound medicine 400ml, 100ml ethanol is added, stirs and evenly mixs into honeysuckle herbal mixture system Agent, it is fitted into medicine watering can or sprayer and can be used to the flushing or spraying of animal injuries.

The preparation of embodiment 8- honeysuckles herbal mixture externally applied anti-inflammation electuary and spray

Precise honeysuckle Chinese traditional compound medicine 800ml, 200ml ethanol is added, stirs and evenly mixs into honeysuckle herbal mixture system Agent, it is fitted into medicine watering can or sprayer and can be used to the flushing or spraying of animal injuries.

The preparation of embodiment 9- honeysuckle herbal mixture externally applied anti-inflammation pulvis

Precise honeysuckle Chinese traditional compound medicine 200ml, medical starch 100g；The two mixes in room temperature and air-dried, grind Honeysuckle herbal mixture externally applied anti-inflammation pulvis.

The preparation of embodiment 10- honeysuckle herbal mixture externally applied anti-inflammation pulvis

Precise honeysuckle Chinese traditional compound medicine 400ml, medical starch 200g；The two mixes in room temperature and air-dried, grind Honeysuckle herbal mixture externally applied anti-inflammation pulvis.

The preparation of embodiment 11- honeysuckle herbal mixture externally applied anti-inflammation paints

Precise honeysuckle Chinese traditional compound medicine 800ml, is heated and flashes to paste；Mixed with medical glycerine 50ml in room temperature Even honeysuckle herbal mixture externally applied anti-inflammation paint.

The preparation of embodiment 12- honeysuckle herbal mixture externally applied anti-inflammation paints

Precise honeysuckle Chinese traditional compound medicine 400ml, is heated and flashes to paste；With medical glycerine 100ml in room temperature Mix honeysuckle herbal mixture externally applied anti-inflammation paint.

The preparation of embodiment 13- slenderstalk dicranostigma herb preparations

(1) slenderstalk dicranostigma herb dry powder 500g is accurately weighed with electronic balance, is loaded in multiple multilayer gauze bags, is tightened sack and be placed in porcelain In pot, add two and steam water 1600ml, absolute ethyl alcohol 400ml (analysis is pure), pH is adjusted extremely with hydrochloric acid (analysis is pure) after stirring 2.0, sealing immersion.Each medicine bag is fully extruded once every 30 minutes.Medicine bag is so soaked into 24h.

(2) by the medical liquid squeezing in husky cloth medicine bag into pot, filtering liquid medicine simultaneously abandons precipitation；By the decoction after filtering and Medicine bag is together placed in pot, and 2h is boiled in heating, and after fluid temperature is down to 25 C °, the decoction in husky cloth medicine bag is screwed onto into pot In pot, filter the decoction in pot and abandon precipitation；Then the pH value of decoction is adjusted to 11.0 with 1N NaOH, filtering precipitation is standby With；After medicine liquid heating is boiled into 2h, multiply heat filtering decoction and abandon precipitation, decoction adjusts pH to 2.0 with 1N hydrochloric acid, and filtering precipitates standby With；Decoction pour out 4 C ° save backup.

（3）Medicine bag is placed in pot again, repeat step（1）And step（2）Soak, boil, adjusting pH value, decoction is poured out 4 C ° save backup.So it is repeated 5 times.

（4）Medicine bag is abandoned, merges 5 gained decoctions, adds 50ml phenmethylols and 50ml glycerine, will with 1N NaOH (analysis is pure) Decoction pH value is adjusted to 5.0, medicine liquid heating evaporation is carried out being concentrated into 500ml, after fluid temperature is down to room temperature, with above-mentioned steps Standby drug precipitation is mixed into suspension, and slenderstalk dicranostigma herb traditional Chinese medicine for external application is produced after autoclaving, puts 4C ° and saves backup.

The preparation of embodiment 14- slenderstalk dicranostigma herb preparations

(1) slenderstalk dicranostigma herb dry powder 1000g is accurately weighed with electronic balance, is loaded in multiple multilayer gauze bags, is tightened sack and be placed in In porcelain pot, add two and steam water 2000ml, absolute ethyl alcohol 500ml (analysis is pure), pH is adjusted with hydrochloric acid (analysis is pure) after stirring To 3.0, sealing is soaked.Each medicine bag is fully extruded once every 30 minutes.Medicine bag is so soaked into 12h.

(2) by the medical liquid squeezing in husky cloth medicine bag into pot, filtering liquid medicine simultaneously abandons precipitation；By the decoction after filtering and Medicine bag is together placed in pot, and 1.5h is boiled in heating, and after fluid temperature is down to 20 C °, the decoction in husky cloth medicine bag is screwed onto In pot, filter the decoction in pot and abandon precipitation；Then the pH value of decoction is adjusted to 10.0 by 1N NaOH, and filtering precipitation is standby With；After medicine liquid heating is boiled into 1.5h, multiply heat filtering decoction and abandon precipitation, decoction adjusts pH to 3.0, filtering precipitation with 1N hydrochloric acid It is standby；Decoction pour out 0 C ° save backup.

（3）Medicine bag is placed in pot again, repeat step（1）And step（2）Soak, boil, adjusting pH value, decoction is poured out 4 C ° save backup.So it is repeated 3 times.

（4）Abandon medicine bag, merge 3 gained decoctions, add 150ml phenmethylols and 150ml glycerine, with 1N NaOH (analysis is pure) Decoction pH value is adjusted to 6.0, medicine liquid heating evaporation is carried out being concentrated into 1000ml, it is and above-mentioned after fluid temperature is down to room temperature The standby drug precipitation of step is mixed into suspension, and slenderstalk dicranostigma herb traditional Chinese medicine for external application is produced after autoclaving, puts 4C ° and saves backup.

The preparation of embodiment 15- slenderstalk dicranostigma herbs Chinese medicine external anti-inflammatory electuary or spray

Precise slenderstalk dicranostigma herb preparation 400ml, add 100ml ethanol, stir and evenly mix into slenderstalk dicranostigma herb preparation, load medicine watering can or The flushing or spraying of animal injuries are can be used in sprayer.

The preparation of embodiment 16- slenderstalk dicranostigma herbs Chinese medicine external anti-inflammatory electuary or spray

Precise slenderstalk dicranostigma herb preparation 800ml, add 200ml ethanol, stir and evenly mix into slenderstalk dicranostigma herb preparation, load medicine watering can or The flushing or spraying of animal injuries are can be used in sprayer.

The preparation of embodiment 17- slenderstalk dicranostigma herb Chinese medicine external anti-inflammatory pulvis

Precise slenderstalk dicranostigma herb preparation 200ml, medical starch 100g；The two mixes in room temperature and air-dried, grind outside slenderstalk dicranostigma herb With anti-inflammatory pulvis.

The preparation of embodiment 18- slenderstalk dicranostigma herb Chinese medicine external anti-inflammatory paints

Precise slenderstalk dicranostigma herb preparation 400ml, is heated and flashes to paste；With medical glycerine 50ml room temperature mix it is bald Sore spends externally applied anti-inflammation paint.

Embodiment 19- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 200g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 200g, ethanol 100ml.

Embodiment 20- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 300g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 100g, ethanol 200ml.

Embodiment 21- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 100g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 300g, ethanol 100ml.

Embodiment 22- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 300g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 300g, glycerine 50ml.

Embodiment 23- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 200g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 200g, medical starch 100ml.

Embodiment 24- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

Raw material forms：The concentration paste 100g of the slenderstalk dicranostigma herb preparation of embodiment 13, the honeysuckle Chinese traditional compound medicine of embodiment 2 Concentrate paste 100g, medical starch 200ml.

The preparation of embodiment 25- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparations

（1）Slenderstalk dicranostigma herb preparation and honeysuckle Chinese traditional compound medicine are separately added into 100ml sterilizings two according to formula ratio and steam water dissolving Afterwards, it is mixed and heated to 75-70 DEG C and stirs；

（2）When equitemperature is down to 25 DEG C, auxiliary material is added, stirs and evenly mixs into slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation, loads medicine The flushing or spraying of animal injuries are can be used in thing watering can or sprayer（Electuary and spray）.

The preparation of embodiment 26- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation pulvis

The concentration paste 200g of precise slenderstalk dicranostigma herb preparation, the concentration paste 200g of honeysuckle Chinese traditional compound medicine, medical shallow lake Powder；After slenderstalk dicranostigma herb preparation and honeysuckle Chinese traditional compound medicine are separately added into the steaming water dissolving of 100ml sterilizings two, with the medical shallow lakes of 200g Powder mix air-dry, grind slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation pulvis.

The preparation of embodiment 27- slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation paints

The concentration paste 200g of precise slenderstalk dicranostigma herb preparation, the concentration paste 200g of honeysuckle Chinese traditional compound medicine；With it is medical Glycerine 50ml room temperature mix slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation paint.

《Effect test》

24 DEG C of cultures for being applied to saccharomycete of cultivation temperature in experiment, 37 DEG C of cultures for being applied to other bacterium.

1. experimental section

The suppression microbe to screen experiment of 1.1 Chinese herbal medicine difference composing prescription preparations

1.1.1 the suppression microbe to screen trial test of Chinese herbal medicine difference composing prescription preparation

1.1.1.1 the capsule of weeping forsythia, cordate houttuynia, radix scutellariae and the screening trial test of golden cypress reciprocation

1.1.1.1.1 horizontal factor table

The capsule of weeping forsythia, cordate houttuynia, radix scutellariae and golden cypress reciprocation horizontal factor table are referring to table 1.

1.1.1.1.2 the preparation of preparation：

Preparation process is prepared by the methods described of embodiment 4, and it is experiment preparation that different preparations finally is concentrated by evaporation into 1 times.

1.1.1.1.3 experimental method：

With orthogonal design table L₁₆(4⁵) it is that horizontal factor combination carries out different preparation preparations, by the salmonella paratyphi of experiment Strain after purification, be inoculated in THB nutrient broths, after 37 DEG C or 24 DEG C culture 24h, is counted respectively with plate count It is configured to 10000 cfu/ml strain suspensions；By the μ l of strain suspensions 100, be separately added into each preparations of 6ml and THB nutrient broths by 1:In culture broth prepared by 1 ratio, counted after 37 DEG C of culture 16h with plate count.Finally calculate inhibiting rate.

1.1.1.1.4 experimental result is with discussing

The capsule of weeping forsythia, cordate houttuynia, radix scutellariae and golden cypress reciprocation experimental result are shown in Table 2.

It can be seen that from the capsule of weeping forsythia, cordate houttuynia, radix scutellariae and golden cypress reciprocation experimental result：It is yellow from factor, capsule of weeping forsythia ＞ A kind of reed mentioned in ancient books ＞ golden cypress ＞ cordate houttuynias；From level, the 4th of the capsule of weeping forsythia is horizontal best, and the 2nd of radix scutellariae is horizontal best, and the 3rd of golden cypress is horizontal Preferably, cordate houttuynia the 3rd is horizontal best.According to the principle of orthogonal experiment, we select capsule of weeping forsythia 50g, cordate houttuynia 25g, radix scutellariae 37.5g With the proportional basis that golden cypress 37.5g is further progress honeysuckle Chinese traditional compound medicine.

1.1.1.2 honeysuckle Chinese traditional compound medicine reciprocation screens trial test

1.1.1.2.1 horizontal factor table

Honeysuckle, Radix Isatidis, radix glycyrrhizae and rhizoma atractylodis reciprocation horizontal factor table are referring to table 3.

1.1.1.2.2 the preparation of preparation：

Preparation process is prepared by the methods described of embodiment 4.

1.1.1.2.3 experimental method：

On the basis of the experiment of the capsule of weeping forsythia, cordate houttuynia, radix scutellariae and golden cypress reciprocation, with capsule of weeping forsythia 50g, cordate houttuynia 25g, radix scutellariae Medicine based on 37.5g and golden cypress 37.5g, with orthogonal design table L above₁₆(4⁵) match somebody with somebody for the different preparations of horizontal factor combination progress System, the salmonella paratyphi strain of experiment after purification, be inoculated in THB nutrient broths, 37 DEG C or 24 DEG C culture 24h Afterwards, with plate count count respectively and be configured to 10000 cfu/ml strain suspensions；By the μ l of strain suspensions 100, difference Add each preparations of 6ml and press 1 with THB nutrient broths:In culture broth prepared by 1 ratio, plate count is used after 37 DEG C of culture 16h Method is counted.Finally calculate inhibiting rate.

1.1.1.2.4 experimental result is with discussing

Honeysuckle Chinese traditional compound medicine reciprocation experimental result is referring to table 4.

Can be with from the honeysuckle in honeysuckle Chinese traditional compound medicine, Radix Isatidis, radix glycyrrhizae and rhizoma atractylodis reciprocation experimental result Find out：From factor, honeysuckle ＞ Radix Isatidis ＞ radix glycyrrhizae ＞ rhizoma atractylodis；From level, the 4th of honeysuckle is horizontal best, plate The 4th of blue root is horizontal preferably, and the 3rd of radix glycyrrhizae is horizontal best, and rhizoma atractylodis the 3rd are horizontal preferably but little with the 2nd horizontal difference.According to just Hand over experiment principle, with reference to the capsule of weeping forsythia, cordate houttuynia, radix scutellariae, golden cypress reciprocation experimental result and honeysuckle, Radix Isatidis, radix glycyrrhizae and Rhizoma atractylodis reciprocation experimental result, the formula that we filter out claim 1 are the underlying scale of honeysuckle Chinese traditional compound medicine Formula.

1.1.1.3 slenderstalk dicranostigma herb herbal mixture external preparation interaction screening trial test

1.1.1.3.1 the preparation of preparation：

Honeysuckle Chinese traditional compound medicine is prepared by the methods described of embodiment 4, and slenderstalk dicranostigma herb preparation is prepared by the methods described of embodiment 13, Finally different preparations is concentrated by evaporation into paste and air-dried as experimental drug thing.

1.1.1.3.2 experimental method：

Following table carries out different formulations preparation preparation, and the aqueous solution that the medicine of different formulations is made into 400ml is used to test.Will be real The salmonella paratyphi strain tested after purification, be inoculated in THB nutrient broths, after 37 DEG C or 24 DEG C culture 24h, respectively Carried out counting the strain suspensions for being configured to 10000 cfu/ml with plate count；By the μ l of strain suspensions 100,6ml is separately added into Each preparation presses 1 with THB nutrient broths:In culture broth prepared by 1 ratio, carried out after 37 DEG C of culture 16h with plate count Count.Finally calculate inhibiting rate.

1.1.1.3.2 experimental result is with discussing

Traditional Chinese medicine for external application interaction experimental result is shown in Table 5.

To ensure that experimental result is obvious, this experiment will carry out 2 times of dilution using concentration, can be more although bacteriostasis rate is low The difference between the bacteriostasis rate of each formula can be clearly seen.In terms of traditional Chinese medicine for external application interaction experimental result, slenderstalk dicranostigma herb is multiple Square traditional Chinese medicine for external application（The compound external-use preparation of slenderstalk dicranostigma herb preparation and honeysuckle Chinese traditional compound medicine）Suppress paratyphoid Salmonella The effect of bacterium is better than single slenderstalk dicranostigma herb preparation and single honeysuckle compound external-use preparation；Slenderstalk dicranostigma herb herbal mixture external preparation The effect that formula 4 suppresses salmonella paratyphi is best, therefore will be formulated 4 bases for being preferably slenderstalk dicranostigma herb herbal mixture external preparation Plinth ratio formula.

1.1.2 the suppression microbe to screen experiment of Chinese herbal medicine difference composing prescription preparation

1.1.2.1 prescription forms：The Chinese herbal medicine component of different preparations is shown in Table 6.

1.1.2.2 the preparation of different preparations：

Preparation I, preparation II and preparation III are respectively the formula of embodiment 1, embodiment 2 and embodiment 3, and preparation process is according to implementation The same procedure of example 4 is prepared；Preparation IV is prepared by the methods described of embodiment 13；Preparation V, the preparation of preparation VI and VII are by real Apply the methods described of example 25 and be formulated as spray.

1.1.2.3 experimental method：

The different microorganisms kind strain of experiment is carried out after purification, to be inoculated in corresponding THB nutrient broths or yeast nutrition meat soup In, after 37 DEG C or 24 DEG C culture 24h, with plate count count respectively and be configured to 10000 cfu/ml microorganism and hang Liquid；Respectively by each μ l of microorganism suspension 100, each preparations of 6ml are separately added into corresponding nutrient broth by 1:Prepared by 1 ratio In culture broth, counted after 37 DEG C or 24 DEG C culture 16h with plate count.Finally calculate inhibiting rate.

1.1.2.4 experimental result：Each preparation is shown in Table 7 to the bacteriostasis rate of different microorganisms, and different microorganisms are respectively yeast Bacterium, staphylococcus aureus, Streptococcus hemolyticus, Streptococcusagalactiae, O2 Escherichia coli, K88 Escherichia coli, paratyphoid Salmonella Bacterium, Pasteurella.

1.1.2.5 discuss：

Honeysuckle Chinese traditional compound medicine（Preparation I, preparation II and preparation III）With slenderstalk dicranostigma herb externally applied anti-inflammation preparation（Preparation VI）Have Good antimicrobial effect, but the pedigree acted on is different.Slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray（Preparation VII）It can make Antimicrobial effect increase of preparation and anti-microbial effect pedigree are wider.Therefore, honeysuckle is filtered out according to experimental result Chinese traditional compound medicine, slenderstalk dicranostigma herb externally applied anti-inflammation preparation and slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray are scorching for treatment animal skin Three kinds of Chinese medicinal herb external anti-inflammation formulations of disease, ulcer and wound.

According to experimental result above, we are honeysuckle with the minimum preparation I of honeysuckle Chinese traditional compound medicine Chinese medicine dose The representative formula of Chinese traditional compound medicine；With the preparation that antimicrobial effect in slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray is minimum V is the representative formula of slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray, carries out following follow-up effect experiment.We are answered with honeysuckle Square Chinese medicine preparation Chinese medicine dose highest preparation III is the representative formula of honeysuckle Chinese traditional compound medicine；Outside with slenderstalk dicranostigma herb herbal mixture With the representative formula that the preparation VI in anti-inflammatory spray is slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray, following family's rabbit skin thorn is carried out Swash experiment.

Inactivation test of 1.2 3 kinds of Chinese medicinal herb external anti-inflammation formulations to ORF viruses

1.2.1 materials and methods

Material：Honeysuckle Chinese traditional compound medicine, slenderstalk dicranostigma herb external preparation and slenderstalk dicranostigma herb herbal mixture external preparation are respectively according to reality Example 4, embodiment 13, the methods described of embodiment 25 is applied to prepare；The HCE poison that Orf viruses preserve for Gansu Livestock and Veterinary Inst. Strain（Gansu Livestock and Veterinary Inst. is that the HCE kinds strain that China Institute of Veterinary Drug Control specifies preserves unit.）；Experiment is with goat by Gansu Province Pingliang The 3-6 monthly age health lambs that city Kongtong District Bai Miao townshiies raiser provides.

The processing of HCE strains：Accurately claim 200mg HCE scabs block malicious with balance, with Hank ' the s liquid containing 1000 units/ml 20ml grinding scab block poison, 24h is acted at 4 DEG C, freeze thawing 3 times, 3000 revs/min centrifuge 10 points, take supernatant, -80 DEG C save backup. Sheep body attacks 6h before poison, with each group different pharmaceutical（Experimental group）Or Hank ' s liquid (control group) carries out 10 times to the venom after defrosting It is diluted to 10^-1、10^-2、10^-3、10^-4、10^-5, 4 DEG C of effects are standby.

Sheep body attacks malicious method：4 groups are randomly divided into after testing sheep overbit piercing by 60, each group is respectively with 10^-1、10^-2、10^-3、 10^-4、10^-5Venom again carries out oral cavity scratch inoculation 0.2ml to experiment sheep, and each venom concentration is repeated 3 times；It is inoculated with 7d-10d After observe result.

1.2.2 result

There is red and swollen different degrees of oral mucosa, ulcer and abscess after 15 sheep inoculation 7d-10d of control group；Experimental group 45 Sheep, except slenderstalk dicranostigma herb externally applied anti-inflammation preparation experimental group 10^-1Venom concentration has 1 sheep to occur outside oral mucosa redness, remaining 44 Sheep is tested without disease symptom.Test result indicates that honeysuckle Chinese traditional compound medicine, slenderstalk dicranostigma herb externally applied anti-inflammation preparation and slenderstalk dicranostigma herb Herbal mixture externally applied anti-inflammation spray has good deactivation to ORF viruses.

1.3 rabbit skin irritation tests

Animal packet and experiment pre-treatment：Take healthy white rabbit 20, regular grade, body weight 2.0-2.5 kg, male and female half and half, 5 d are raised before experiment and observe diet and behavior, 4 groups are randomly divided into by sex, body weight if normal（1 group is compareed, experiment 3 Group）, every group 5.24 h cut off family's rabbit back backbone both sides hair before each group administration, are then lost hair or feathers with razor head；Hair removal section Right side skin is divided into small " # " shape to sterilize syringe needle, is degree to oozing of blood, as damaged skin group, left side is intact skin group.

Pharmaceutical preparation tests pre-treatment：Respectively by honeysuckle Chinese traditional compound medicine, slenderstalk dicranostigma herb external application made from embodiment 1-3 Anti-inflammation formulation and slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation are concentrated by evaporation into paste, are used in mixed way with 20% sterile glycerol；Control Group substitutes ointment with sterile glycerol.

Medication：All on right side, hair removal section is coated with ointment paint 2g to each test group of animals, and control group applies glycerine about 2g and made Control.Local complexity gauze and antistaling film after administration, and with immobilization with adhesive tape, medicine is kept with removing yarn after skin contact 4h Cloth and antistaling film, then wash away left drug with warm water.It is administered once daily, continuous 5d.1h and again after medicine is removed every time Skin condition is observed before secondary administration.1,24,48 and 72 h are observed and are recorded spreader portion again after last dose and removal medicine Position whether there is erythema and oedema and recovery situation, and its evaluation criterion is shown in Table 8 and table 9.

As a result：Learnt by experimental administration and observation, 1h and be administered again after medicine is removed every time on the left of each group skin 1,24,48 and 72h after preceding and last dose, visually observe without the skin wound repair symptom such as obvious erythema, oedema；Right side After percutaneous drug delivery, the 1st day visible abraded area of 3 experimental groups and control group has slight redness, recovers completely just to the 4th day Often, thereafter every time remove medicine after 1h and again administration before and last dose after 1,24,48 and 72h, visually observe without bright Skin irritation reaction symptom, every score values such as aobvious erythema, oedema are zero.

1.4 each preparation Stability experiments

Take honeysuckle Chinese traditional compound medicine, slenderstalk dicranostigma herb externally applied anti-inflammation preparation and the slenderstalk dicranostigma herb compound that various embodiments of the present invention prepare Chinese medicine external anti-inflammatory spray, stored 1 year under the conditions of 4 DEG C, clinical therapeutic efficacy is unchanged；The slenderstalk dicranostigma herb being configured to them is answered Square Chinese medicine external anti-inflammation formulation, clinical therapeutic efficacy are also unchanged.

2. clinical practice

2.1 material

2.1.1 experimental animal

Sheep is treated to be provided by the plant on the ground such as Gansu, Shaanxi, Ningxia and raiser.

2.1.2 preparation

Take honeysuckle Chinese traditional compound medicine, slenderstalk dicranostigma herb preparation, slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation prepared by the embodiment of the present invention Pulvis.The Chinese herbal medicine component of different preparations is shown in Table 6.

2.2 method

2.2.1 therapeutic test of the slenderstalk dicranostigma herb preparation to animal surgery disease

To animal wound caused by various mechanical reasons or the wound infected, papule, abscess and ulcer, first with sterile life Manage salt solution cleaning and rinse injury, dry physiological saline is dipped in sterile dry absorbent cotton, for injury that is easily fixed and easily applying, Anti-inflammation formulation is applied to wound position, with Sha Bu and immobilization with adhesive tape it, 1 time a day, treat 3-5d；For not being fixed easily and being not easy The injury of painting, with by daily 2-3 times of anti-inflammatory spray, treat 3-5 d.

To the disease sites for the animal for having suffered from mange, first with removing surface cortex after 60 DEG C or so of warm water hot compress 10min Layer, is then applied in wound face with Chinese medicine external anti-inflammation formulation, with Sha Bu and immobilization with adhesive tape, 1 time a day, treats 3-5 d；It is right In the injury for not being fixed easily and being not easy to apply, with by daily 2-3 times of slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation spray, treatment 3- 5 d。

The fistula formed to animal by infection, first with sterile saline and anti-inflammation formulation cleaning down cleaning fistula Portion, then Chinese medicine external anti-inflammatory pulvis is filled in inside fistula, finally with Sha Bu and immobilization with adhesive tape fistula mouth, 1 time a day, treatment 3—5 d。

2.2.2 Orf therapeutic tests

To having suffered from Orf (sheep contagiousness dermatitis impetiginosa) and the sheep and goat of obvious clinical symptoms occur, with difference Externally applied anti-inflammation preparation carry out therapeutic test.To papule, blister, warts and ulcer caused by Orf, need to first scratch cleaning papule, Blister, warts and ulcer wound surface,（Do not processed typically for the position for forming excipuliform crust in convalescence.）Use physiological saline Dipped in again with sterile dry absorbent cotton dry after flushing, externally applied anti-inflammation preparation is applied or be sprayed on papule, blister, warts and position of festering, daily 2-3 times, treat 3-5d.（For injury that is easily fixed and easily applying, externally applied anti-inflammation preparation can be applied to wound position, With Sha Bu and immobilization with adhesive tape it, 1 time a day, treat 3-5d.）Sick sheep recovers normal for healing in 5d.

2.3 results are with discussing

2.3.1 result

2.3.1.1 therapeutic test result of the externally applied anti-inflammation preparation to animal surgery disease

Therapeutic test of the externally applied anti-inflammation preparation to animal surgery wound, papule, abscess, ulcer, fistula and mange the results are shown in Table 10- Table 11.From following table, the healing percentage difference of preparation I, preparation IV and preparation V to animal surgery wound, fistula and mange For 94.3,95.1 and 98.1%；85.7th, 85.7 and 87.5%；94.7th, 89.5 and 100.0%.Therapeutic test result shows, of the invention Externally applied anti-inflammation preparation has good therapeutic action to animal surgery wound, fistula and mange.

2.3.1.2 different preparations are to sheep of virus Orf treatment results

The slenderstalk dicranostigma herb preparation of different prescriptions is seen the above table to Orf treatment results.As seen from the above table, Chinese medicine preparation I, IV, V pair of lamb Cure rate with adult sheep Orf is respectively 87.6%, 91.5%, 92.1% and 89.2%, 93.3% and 95.0%.Orf therapeutic test knots Fruit shows that externally applied anti-inflammation preparation has good therapeutic action to Orf.

2.3.2 discuss

With the globalization of world commerce, the complication of the incoming and old disease of new Animal diseases makes the preventing and treating of livestock and poultry Increasingly it is difficult, while zoonosis increases, its complexity increases, particularly the eruption and prevalence and animal disease as bird flu etc. Disease is relevant, adds the difficulty of prevention and cure of Animal diseases.Animal health degree is not only related to the quality of life of people, and relation To the sanitarian safety of society.Although the development of modern medicine, materia medica and molecular biology, the preventing and treating to Animal diseases carries Supply a variety of prevention and controls, but can not still solve in many aspects in face of complicated and diversified Animal diseases-such as the resistance of antibiotic With residue problem etc..And the traditional Chinese herbal medicine in China has the effect of uniqueness to the insurmountable disease of many modern medicines and easily existed Metabolic conversion and elimination in the inner and outer boundary's environment of animal body, served using the unique Chinese herbal medicine in China and the achievement obtained Animal husbandry and to promote the well-being of mankind be the obligation and responsibility of our veterinarians.

It was found from therapeutic test result of the externally applied anti-inflammation preparation to animal surgery wound, mange and fistula, externally applied anti-inflammation system Agent has extraordinary therapeutic effect to animal surgery wound, mange and fistula, and finds externally applied anti-inflammation system over the course for the treatment of Agent not only has killing or inhibiting effect but also healing and the reparation of tissue to surgery wound, mange and fistula to pathogenic microorganism There is facilitation.From externally applied anti-inflammation preparation to Orf treatment results in terms of, preparation V, preparation VI and preparation VII to lamb and adult sheep Orf have a good therapeutic effect, preparation VII is optimal to Orf therapeutic effects.

Claims

A kind of 1. Chinese medicine external anti-inflammation formulation, it is characterised in that：It is honeysuckle Chinese traditional compound medicine, by the original of following parts by weight Material is made：Honeysuckle 60-80 parts, rhizoma atractylodis 30-60 parts, capsule of weeping forsythia 60-80 parts, radix scutellariae 60-80 parts, golden cypress 60-80 parts, Radix Isatidis 60-80 parts, cordate houttuynia 30-60 parts and radix glycyrrhizae 30-60 parts.
A kind of 2. Chinese medicine external anti-inflammation formulation, it is characterised in that：It is the bald of slenderstalk dicranostigma herb preparation and honeysuckle Chinese traditional compound medicine Sore spends herbal mixture externally applied anti-inflammation preparation, is made from the following raw materials in parts by weight, slenderstalk dicranostigma herb preparation 1-3 parts, in honeysuckle compound Medicine preparation 1-3 parts and auxiliary material, auxiliary material are ethanol 1-2 parts or glycerine or medical starch 1-2 parts.
3. a kind of preparation method of Chinese medicine external anti-inflammation formulation described in claim 1, it is characterised in that comprise the following steps：

（1）Honeysuckle, rhizoma atractylodis, the capsule of weeping forsythia, radix scutellariae, golden cypress, Radix Isatidis, cordate houttuynia and radix glycyrrhizae are weighed according to formula ratio, in addition to rhizoma atractylodis, Other medicines are poured into porcelain pot, and water 1600-2000ml, absolute ethyl alcohol 400-500ml, phenmethylol 5-15ml are added in pot With glycerine 5-15ml, pH to 5.0-6.0, sealing immersion are adjusted with hydrochloric acid after stirring；Stirred once every 30-60 minutes；It is grey Art seals immersion with 100-200ml alcohol in another container；Drug immersion 12-24h；

（2）After drug immersion, rhizoma atractylodis immersion is incorporated into pot with other drugs soak and stirred, 1.5- is boiled in heating 2h, after fluid temperature is down to 50-60 C °, the decoction 0-4 C ° filtered in pot are saved backup and are abandoned precipitation；

（3）Repeat the above steps（1）And step（2）3-5 times；

（4）The dregs of a decoction are abandoned, merges above-mentioned each gained decoction, medicine liquid heating evaporation is carried out being concentrated into 250-500ml, when decoction temperature After degree is down to room temperature, product is produced after filtration high pressure sterilizing.
A kind of 4. preparation method of Chinese medicine external anti-inflammation formulation according to claim 3, it is characterised in that：Said preparation is electuary Or spray, honeysuckle Chinese traditional compound medicine 4-8 parts, 1-2 part ethanol is added, stirs and evenly mixs and forms.
A kind of 5. preparation method of Chinese medicine external anti-inflammation formulation according to claim 3, it is characterised in that：Said preparation is powder Agent, honeysuckle Chinese traditional compound medicine 2-4 parts, medical starch 1-2 parts, the two mixes in room temperature and air-dried, grind.
A kind of 6. preparation method of Chinese medicine external anti-inflammation formulation according to claim 3, it is characterised in that：Said preparation is painting Agent, honeysuckle Chinese traditional compound medicine 4-8 parts, is heated and flashes to paste；Mixed with medical glycerine 0.5-1 parts in room temperature and be Into.
A kind of 7. preparation method of Chinese medicine external anti-inflammation formulation described in claim 2, it is characterised in that：Slenderstalk dicranostigma herb system therein Agent is prepared by following steps,

(1) slenderstalk dicranostigma herb dry powder 500-1000 parts are weighed, loads in multilayer gauze bag, is placed in porcelain pot, two is added and steams water 1600-2000ml, absolute ethyl alcohol 400-500ml, pH to 2.0-3.0, sealing immersion 12-24h are adjusted with hydrochloric acid after stirring;

(2) by the medical liquid squeezing in husky cloth medicine bag into pot, filtering liquid medicine simultaneously abandons precipitation；By the decoction after filtering and medicine bag one With being placed in pot, 1.5-2h is boiled in heating, and after fluid temperature is down to 20-25 C °, the decoction in husky cloth medicine bag is screwed onto into pot In pot, filter the decoction in pot and abandon precipitation；Then the pH value of decoction is adjusted to 10.0-11.0 with NaOH, filtering precipitation is standby With；After medicine liquid heating is boiled into 1.5-2h, multiply heat filtering decoction and abandon precipitation, decoction adjusts pH to 2.0-3.0 with hydrochloric acid, and filtering is sunk Form sediment standby；Decoction is poured out 0-4 C ° and saved backup；

（3）Medicine bag is placed in pot again, repeat step（1）And step（2）In immersion, boil, adjust pH value step, so Repeat 3-5 times；

（4）Abandon medicine bag, merge each time obtained by decoction, add 50-150ml phenmethylols and 50-150ml glycerine, with NaOH by decoction pH Value is adjusted to 5.0-6.0, and medicine liquid heating evaporation is carried out being concentrated into 500-1000ml, and above-mentioned after fluid temperature is down to room temperature The standby drug precipitation of step is mixed into suspension, and product is produced after autoclaving.
A kind of 8. preparation method of Chinese medicine external anti-inflammation formulation according to claim 7, it is characterised in that：The slenderstalk dicranostigma herb Preparation is electuary or spray, slenderstalk dicranostigma herb preparation 4-8 parts, adds 1-2 part ethanol, stirs and evenly mixs and form.
A kind of 9. preparation method of Chinese medicine external anti-inflammation formulation described in claim 2, it is characterised in that：Slenderstalk dicranostigma herb therein is answered The preparation process of square Chinese medicine external anti-inflammation formulation is as follows：

（1）It will be mixed after slenderstalk dicranostigma herb preparation, the dissolving of honeysuckle Chinese traditional compound medicine, be heated to 75-70 DEG C；

（2）When equitemperature is down to room temperature, auxiliary material is added, stirs and evenly mixs into slenderstalk dicranostigma herb herbal mixture externally applied anti-inflammation preparation.
10. a kind of Chinese medicine external anti-inflammation formulation described in claim 1 or 2 is in treatment animal oral cavity skin ulcer, inflammation and outer The application of wound.