CN107890486B

CN107890486B - Traditional Chinese medicine external anti-inflammation preparation and preparation method and application thereof

Info

Publication number: CN107890486B
Application number: CN201711258828.7A
Authority: CN
Inventors: 党岩; 梁剑平; 马志宏; 张莉敏; 常攀峰; 孙春香; 苟想珍
Original assignee: Gansu Institute Of Animal Husbandry Veterinary Medicine
Current assignee: Gansu Institute Of Animal Husbandry Veterinary Medicine
Priority date: 2017-12-04
Filing date: 2017-12-04
Publication date: 2021-03-05
Anticipated expiration: 2037-12-04
Also published as: CN107890486A

Abstract

The invention discloses a traditional Chinese medicine external anti-inflammatory preparation as well as a preparation method and application thereof, belongs to the field of veterinary medicines, and aims to solve the problems of low content of active ingredients of medicines, unstable treatment effect and high production cost of the existing external preparation. The externally applied anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea pedis is prepared from the following raw materials, by weight, 1-3 parts of the tinea pedis preparation, 1-3 parts of the compound traditional Chinese medicine preparation of honeysuckle and auxiliary materials. The invention combines the hydrochloric acid salt preparation of the tinea capitis and the compound traditional Chinese medicine preparation of honeysuckle and the like, has good synergistic effect between the hydrochloric acid salt preparation of the tinea capitis and the compound traditional Chinese medicine preparation of the honeysuckle and the like, has good treatment effect on ulcer, inflammation and trauma of oral cavity and skin of animals, and has the effective rate of 100 percent and the cure rate of 85.7-100 percent.

Description

Traditional Chinese medicine external anti-inflammation preparation and preparation method and application thereof

Technical Field

The invention belongs to the field of veterinary medicines, and particularly relates to a traditional Chinese medicine external anti-inflammation preparation as well as a preparation method and application thereof.

Background

At present, under the background of the implementation of the national green environmental protection strategy, the society pays attention to food safety widely, calls the production of pollution-free products and green animal products, and is particularly applied to the development of food-borne animal medicines in the aspects of low toxicity, no residue, high efficiency and the like.

Inflammation, ulcer and trauma of oral cavity and skin of animals refer to pimples, inflammation, ulcer, hemorrhage, suppuration and external wound infection of animals caused by internal and external diseases and infectious diseases, and are caused by various pathogenic microorganism infections of external skin caused by various etiological factors. Some of the diseases are single in animal groups, such as suppurative inflammation and trauma infection, and have little influence on the whole production of livestock and poultry, but some infectious diseases are group, such as contagious pustular dermatitis of sheep, the morbidity is averagely 75%, the morbidity of lambs is up to more than 90%, and some of the mortality can reach about 15%, so that the animal husbandry production is greatly influenced; meanwhile, the diseases such as contagious ecthyma dermatitis of sheep are also diseases of people and livestock, and people, cats, camels, muskrats, reindeer, antelopes, seals, dogs and the like can also infect the diseases, and the diseases also have important influence on public health and environmental protection. The health degree of animals is not only related to the life quality of people, but also related to the safety of public health of society. Despite the advances in modern medicine, pharmacology and molecular biology that provide a variety of treatments for the prevention and treatment of oral and dermal inflammation, ulceration and trauma in animals, the face of the complex and diverse oral and dermal inflammation, ulceration and trauma in animals remains unsolved in many respects.

Although the development of modern medicine, pharmacology and molecular biology provides a plurality of therapeutic drugs and methods for preventing and treating animal skin inflammation, ulcer and trauma, the treatment of animal skin inflammation, ulcer and trauma mainly comprises chemical drugs with stronger toxicity, antibiotics, strong acid and strong base, strong oxidation reducing agent and animal nucleic acid protein denaturant. The chemical drugs, antibiotics, strong acid and strong base, strong oxidation reducing agent and animal nucleic acid protein denaturant are used blindly for a long time, so that the living natural environment of people is seriously polluted; pathogenic microorganisms are increasingly resistant to these drugs; the drugs can seriously damage normal tissue cells of animal skin and are not favorable for the repair and growth of the tissue cells of the animal skin.

Tinea pedis treating flower (Dicranostigma leptopodum(Maxim) Fedde, DLF), is a biennial or perennial herb of the poppy family leptopodium, and is mainly distributed in Gansu, Shaanxi, etc. Since the eighties of the last century, many researchers in China studied the components of the tinea capitis flowers and the pharmacological effects of the tinea capitis flower preparation, and extracted from the tinea capitis flowers include aloperine, isocorydine, corydaline, protopine, allocryptopine, sinomenine and the likeA plurality of compounds. Research shows that the tinea pedis flower has good healing effect on tuberculosis, and the healing effect is over 79.5 percent.

The existing external preparation for treating animal skin inflammation, ulcer, trauma and other diseases has the defects of low content of active ingredients of medicaments, unstable treatment effect, high production cost, relatively complex manufacturing process and difficult clinical treatment operation.

The effective component of the medicine of the Chinese herbal medicine bald sore flower is alkaloid, the dissolution characteristics are that except small molecular alkaloid, other matters are slightly soluble in water, fat-soluble alkaloid has better solubility in organic solvents such as ethanol, chloroform and the like but is slightly soluble in water, and the final product of the external preparation of the bald sore flower is an aqueous or alcoholic solution preparation and paste or powder formed by adding an adjuvant on the basis. Therefore, the preparation method of the preparation of the tinea pedis flower mainly comprises organic solvent extraction, aqueous solvent extraction and various chromatographic methods, and is assisted by ultrasonic oscillation dissolution, distillation and other methods. Zhaoqiang is prepared by soaking powder of tinea pedis pollen in ethanol solution at room temperature under sealed condition, ultrasonic oscillating for dissolving, and evaporating ethanol to obtain ethanol concentrated extract of tinea pedis flower; the preparation method of Wangting uncut jade comprises extracting whole plant of the tinea pedis flower with water, precipitating with ethanol, concentrating, and lyophilizing to obtain preparation; yi Jian Hua and Liangjianping, etc. are prepared by extracting the effective components of the medicine from the powder of the tinea pedis flower with water, concentrating, extracting the effective components with ethanol, and evaporating the ethanol to obtain the water-soluble preparation of the tinea pedis flower.

The effective components of the Chinese herbal medicine of the tinea pedis flower, which are antimicrobial, anti-inflammatory and capable of regulating the immune function of animal organisms, are commonly recognized to be mainly alkaloids at present, and the preparation method of the tinea pedis flower preparation is mainly organic solvent extraction, aqueous solvent extraction and various chromatographic methods at present and is assisted by methods of ultrasonic oscillation dissolution, distillation and the like; and the preparation method is different for different research units of the purpose and the application of users, and the effective components and the standard of the preparation are also different.

Disclosure of Invention

The invention aims to provide a simple, convenient, economic and effective traditional Chinese medicine external anti-inflammatory preparation, which solves the problems of low content of effective ingredients of medicines, unstable treatment effect and high production cost of the existing external preparation.

The invention also aims to provide a preparation method of the traditional Chinese medicine external anti-inflammatory preparation, so as to solve the problem that the manufacturing process of the existing external preparation for treating the tinea pedis is relatively complex.

The invention also aims to provide application of the traditional Chinese medicine external anti-inflammatory preparation.

The technical scheme of the invention is as follows: a traditional Chinese medicine external anti-inflammation preparation is a honeysuckle compound traditional Chinese medicine preparation and is prepared from the following raw materials in parts by weight: 60-80 parts of honeysuckle, 30-60 parts of rhizoma atractylodis, 60-80 parts of fructus forsythiae, 60-80 parts of radix scutellariae, 60-80 parts of cortex phellodendri, 60-80 parts of radix isatidis, 30-60 parts of herba houttuyniae and 30-60 parts of liquorice.

In another embodiment of the invention, the external anti-inflammatory traditional Chinese medicine preparation is a compound traditional Chinese medicine preparation of the tinea pedis flower, which is a compound traditional Chinese medicine preparation of the tinea pedis flower and the honeysuckle flower, and the external anti-inflammatory traditional Chinese medicine preparation takes water-soluble alkaloid hydrochloride as a main component and is supplemented with benzyl alcohol, glycerol and ethanol to improve the solubility and the effect of active ingredients of the medicine. The traditional Chinese medicine is prepared from the following raw materials, by weight, 1-3 parts of a tinea capitis preparation, 1-3 parts of a honeysuckle compound traditional Chinese medicine preparation and an auxiliary material, wherein the auxiliary material is 1-2 parts of ethanol or 1-2 parts of glycerol or medical starch.

The preparation method of the honeysuckle compound traditional Chinese medicine preparation for external use of traditional Chinese medicine for diminishing inflammation comprises the following steps:

(1) weighing honeysuckle, rhizoma atractylodis, fructus forsythiae, scutellaria baicalensis, golden cypress, radix isatidis, houttuynia cordata and liquorice according to the formula, pouring other medicines except the rhizoma atractylodis into a porcelain pot, adding 1600-2000ml of water, 400-500ml of absolute ethyl alcohol, 5-15ml of benzyl alcohol and 5-15ml of glycerol into the pot, uniformly stirring, adjusting the pH value to 5.0-6.0 by using hydrochloric acid, and sealing and soaking; stirring once every 30-60 minutes; soaking rhizoma Atractylodis in 100 and 200ml ethanol in another container under sealed condition; soaking the medicines for 12-24h;

(2) soaking the above materials, mixing rhizoma Atractylodis soaking solution and other medicinal soaking solutions, stirring, boiling for 1.5-2 hr, cooling to 50-60 deg.C, filtering the medicinal liquid in the pot at 0-4 deg.C, and removing precipitate;

(3) repeating the steps (1) and (2) for 3-5 times;

(4) removing the residues, mixing the above obtained medicinal liquids, heating and evaporating the medicinal liquid, concentrating to 250-.

Furthermore, the preparation is a medicinal granule or a spray, and the honeysuckle compound traditional Chinese medicine preparation is prepared by adding 1-2 parts of ethanol into 4-8 parts of honeysuckle compound traditional Chinese medicine preparation and uniformly stirring.

Furthermore, the preparation is powder, 2-4 parts of honeysuckle compound traditional Chinese medicine preparation and 1-2 parts of medical starch, and the honeysuckle compound traditional Chinese medicine preparation and the medical starch are mixed uniformly at room temperature, dried in the air and ground to obtain the honeysuckle compound traditional Chinese medicine preparation.

Further, the preparation is a liniment, and the honeysuckle compound traditional Chinese medicine preparation is heated and evaporated into paste in 4-8 parts; mixing with 0.5-1 part of medical glycerin at room temperature.

In the preparation method of the traditional Chinese medicine external anti-inflammatory preparation, the preparation for treating the tinea pedis is prepared by the following steps,

(1) weighing 1000 parts of the dry powder of the tinea capitis flowers, putting the powder into a multilayer gauze bag, putting the bag into a porcelain pot, adding 1600ml of secondary distilled water and 2000ml of anhydrous ethanol, adjusting the pH value to 2.0-3.0 by hydrochloric acid after uniformly stirring, and sealing and soaking for 12-24h;

(2) extruding the liquid medicine in the gauze bag into a pot, filtering the liquid medicine and removing the precipitate; placing the filtered liquid medicine and the medicine bag in a pot, heating and boiling for 1.5-2h, screwing the liquid medicine in the gauze medicine bag into the pot when the temperature of the liquid medicine is reduced to 20-25 ℃, filtering the liquid medicine in the pot and removing the precipitate; then adjusting the pH value of the liquid medicine to 10.0-11.0 by NaOH, filtering and precipitating for later use; heating the medicinal liquid to boil for 1.5-2h, filtering the medicinal liquid while heating, removing the precipitate, adjusting pH of the medicinal liquid to 2.0-3.0 with hydrochloric acid, and filtering the precipitate for use; pouring out the liquid medicine at 0-4 deg.C, and storing;

(3) placing the medicated bag in a pot, repeating the steps of soaking, boiling and adjusting pH value in steps (1) and (2), and repeating the steps for 3-5 times;

(4) discarding the medicine bag, combining the medicine liquids obtained in each time, adding 50-150ml of benzyl alcohol and 50-150ml of glycerol, adjusting the pH value of the medicine liquid to 5.0-6.0 by NaOH, heating and evaporating the medicine liquid, concentrating the medicine liquid to 500-1000ml, cooling the medicine liquid to room temperature, mixing the medicine liquid and the medicine precipitate prepared in the previous step into suspension, and carrying out autoclaving to obtain the product.

Furthermore, the preparation of the tinea pedis flower is electuary or spray, 4-8 parts of the preparation of the tinea pedis flower is added with 1-2 parts of ethanol and is stirred and mixed evenly.

The preparation method of the traditional Chinese medicine external anti-inflammatory preparation comprises the following steps of:

(1) dissolving the preparation of the tinea pedis flower and the compound traditional Chinese medicine preparation of the honeysuckle flower, mixing, and heating to 75-70 ℃;

(2) when the temperature is reduced to room temperature, adding the auxiliary materials, and uniformly stirring to form the externally-applied anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea pedis.

The traditional Chinese medicine external anti-inflammatory preparation is applied to treating oral ulcer, inflammation and trauma of animals.

The invention solves the problem of environmental pollution of the anti-inflammatory medicament for external use of animals by using the specific low-toxicity and easily-metabolized medicinal components of the traditional Chinese herbal medicines; solves the drug resistance problem of pathogenic microorganisms by using the diversity and the cooperativity of the specific antimicrobial components and the functions of the traditional Chinese herbal medicines; the problem of rapid repair and growth of animal skin damaged tissue cells is solved by the safety of the specific medicinal effective components of the traditional Chinese herbal medicines and the immunoregulation function of the animal organism damaged tissue.

The traditional Chinese medicine external anti-inflammatory preparation is a honeysuckle compound traditional Chinese medicine preparation, according to the compound principle of Chinese herbal medicine monarch, minister, assistant and guide, the honeysuckle compound traditional Chinese medicine preparation uses honeysuckle as a monarch drug and takes forsythia and scutellaria as minister drugs, which are heat-clearing, detoxifying and anti-microbial drugs, so that the detoxifying and anti-microbial action range is enlarged and the action effect is enhanced; the anti-inflammatory, anti-ulcer and anti-microbial effects of the compound prescription taking the heat-clearing, detoxifying and anti-microbial medicines of the phellodendron and the houttuynia cordata as the ministers and the guides are enhanced; the antiviral effect of the compound prescription is enhanced by taking the radix isatidis as a heat-clearing and detoxifying antiviral drug; the atractylodes rhizome which is a damp-clearing and anti-inflammatory medicine is taken as an adjuvant, the atractylodes rhizome which is a minister phellodendron bark and the like are used for synergistically enhancing the effects of clearing damp, inhibiting acid, inhibiting ulcer and protecting mucosa, and the liquorice which is a warm heat-clearing and detoxifying medicine is taken as an adjuvant and a mediation monarch medicine, so that the anti-inflammatory rehabilitation effect of the compound is enhanced; ethanol, medical starch and glycerol are used as formulation adjuvants, so that the preparation has different forms, and is beneficial to the arrival of effective components of the medicine at the disease position and the protection of mucosa.

The invention discloses a traditional Chinese medicine external anti-inflammatory preparation, and a second embodiment is a compound traditional Chinese medicine external anti-inflammatory preparation for treating tinea capitis.

Although the external anti-inflammatory preparation for treating the alopecia areata and the compound traditional Chinese medicine preparation for the honeysuckle have good effects of treating animal skin inflammation, ulcer and trauma, the treatment effects on diseases with the same symptoms caused by various reasons are inconsistent, and the reason for the result is that the antimicrobial pedigree of the single anti-inflammatory preparation for the diseases with the same clinical symptoms caused by various reasons is inconsistent and relatively narrow. The synergistic effect of the external anti-inflammatory preparation of the tinea capitis and the compound traditional Chinese medicine preparation of the honeysuckle on the skin inflammation, ulcer and trauma of animals is utilized to increase the spectrum range of the action of the medicine on the skin inflammation, ulcer and trauma of the animals; enhancing the action efficacy of the medicament on animal skin inflammation, ulcer and trauma is one of the main purposes of developing the externally applied anti-inflammatory preparation of the compound traditional Chinese medicine of the tinea capitis flower.

According to the compound principle of monarch, minister, assistant and guide of Chinese herbal medicines, the externally applied anti-inflammatory preparation of the compound traditional Chinese medicine of the tinea pedis flower is prepared by taking the heat-clearing, detoxifying and antimicrobial medicine of the tinea pedis flower as the monarch and the compound traditional Chinese medicine of the honeysuckle flower as the assistant (the heat-clearing, detoxifying and antimicrobial medicine of the honeysuckle flower, the weeping forsythia capsule and the baical skullcap root as the minister and guide has enlarged detoxifying and antimicrobial action range and enhanced action effect, the phellodendron bark and the heartleaf houttuynia herb as the minister and guide have enhanced anti-inflammatory and anti-ulcer and antimicrobial action effects, the isatis root as the heat-clearing, detoxifying and antiviral medicine as the minister and guide have enhanced antiviral action, the rhizoma atractylodis as the assistant and the phellodendron bark as the dampness-draining, the anti-acid, the ulcer and protective mucosa functions of the compound are enhanced in a synergistic manner, and the liquorice as the minister and assistant; ethanol, medical starch or glycerol are used as formulation adjuvants, so as to endow the preparation with different forms, facilitate the effective components of the medicine to reach the diseased site and protect mucosa.

Honeysuckle, rhizoma atractylodis, forsythia, scutellaria, phellodendron, dyers woad leaf, isatis root, houttuynia and glycyrrhiza are Chinese herbal medicines for clearing heat and removing toxicity, the Chinese herbal medicines contain organic acid, alkaloid, glycoside or glucoside, polysaccharide, flavone, sterol, volatile oil, trace elements and the like, and all have the functions of resisting microorganisms and inflammation, and meanwhile, the Chinese herbal medicines have better synergistic action. The Chinese herbal medicines not only have good antimicrobial effect, but also have the function of regulating the immune system of the animal body so as to promote the wound healing of the animal body. The components in the Chinese herbal medicine are easy to be decomposed and metabolized, and the Chinese herbal medicine has important significance for the green environmental protection and public health of human beings. The test result shows that the honeysuckle, the rhizoma atractylodis, the forsythia, the scutellaria, the phellodendron, the isatis root, the houttuynia cordata, the liquorice and the tinea capitis preparation have good synergistic effects of resisting microorganisms and inflammation and promoting wound healing. The research of the Chinese herbal medicines such as the tinea capitis and the like is carried out for many years, and the compound tinea capitis preparation is developed on the basis of developing the tinea capitis electuary, injection, liniment and oral capsules, so that the problem of difficulty in clinical operation of veterinarians is solved, and the time and the labor are saved on the basis of ensuring the curative effect of treatment.

The effective components of the Chinese herbal medicine of the tinea capitis flower for resisting microorganism, inflammation and immunoregulation comprise sanguinarine, allocryptopine, magnoline, sinomenine and other alkaloids. Sanguinarine in the tinea pedis flower has antibacterial, antitumor, and trypanosome resisting effects. Research results show that the sanguinarine acid sulfate has an inhibiting effect on koff-Wolk trichophyton floccosum at the concentration of 717 mg/L; the antibacterial agent has antibacterial effect on staphylococcus aureus, candida albicans and Microsporum lanuginosum, and the minimum inhibitory concentration on staphylococcus aureus is 1.95 mg/L-3.9 mg/L; the sanguinarine has stronger activity to aeromonas hydrophila, and the minimum inhibitory concentration and the minimum antibacterial concentration of the sanguinarine are 12.5 mg/L and 25 mg/L respectively. The activity to vibrio harveyi, vibrio anguillarum and aeromonas salmonicida is weaker; sanguinarine has anti-vitamin insect effect, and can be used for treating trypanosoma brucei and trypanosoma congolense in blood flowFormation of an inhibitor ED₅₀The 1/40000 solution of sulfate and nitrate still has 100 percent of inhibition effect on the Louis silverworm after 24 hours; sanguinarine has killing effect on fish dactylogyrus, Tetranychus urticae Koch and Oncomelania hupensis; the sanguinarine can kill human leukemia K562, can resist the drug tolerance of cervical cancer cells, has an inhibiting effect on ehrlich ascites tumor, inhibits the growth and transfer of the cervical cancer cells, can induce the apoptosis of p53-mutant and p53-null cloned cancer cell lines, human pancreatic cancer cells AspC-1 and BxPC-3, has an obvious killing effect on liver cancer cells, and has strong cytotoxic activity on human prostate cancer cells proved by in vitro tests. Chelerythrine in the bald sore flower has antibacterial and antitumor effects. The test results prove that the chelerythrine has the inhibition effect on cariogenic bacteria, actinobacillus actinomycetemcomitans and streptomyces variabilis, the inhibition effect on pathogenic bacteria of a plurality of melons, fruits and vegetables, the chelerythrine can induce the apoptosis of human gastric cancer BGC823 cells and human uvea melanoma strains, and the proliferation of cervical cancer cells can be inhibited. In addition, chelerythrine has acute inflammation resisting effect. The magnoline in the fagopyrum crinitum flower has cytotoxicity, antibacterial, antioxidant, and antiinflammatory effects on tumor cell line. Chenjiahui and the like adopt a flat plate method to determine the antibacterial action of beta and alpha isomers of the magnoline, and test results prove that the minimum inhibitory concentration of the beta-magnoline to the hazelnut color penicillium UC-4376 is 10 mu g/disc, and the minimum inhibitory concentration to rice blast bacteria is 100 mu g/disc; the minimum inhibitory concentration of the alpha-magnoline to the hazel-color penicillium is 5 mu g/disc. Allocryptopine in the tinea pedis flower has the effects of resisting tumor, inhibiting bacteria, killing parasite, etc. Allocryptopine has an anti-tumor effect, and inhibition of DNA topoisomerase is probably its anti-tumor mechanism. Allocryptopine has inhibitory effect on various bacteria, and can kill pieris rapae larva, adult Triplostegiae ruber and Culex fatigues. The sinomenine in the tinea pedis flower has the functions of immunoregulation and anti-inflammation. Sinomenine can inhibit LPS and PMA induced mouse spleen lymphocyte proliferation in vivo, and ConA, LPS and anti-CD in vitro₃Inducing mouse spleen lymphocyte proliferation, and increasing rat CD₄／CD₈The ratio is reduced, and the early apoptosis percentage of the splenic lymphocytes of the mice can be obviously increased in vitro. Sinomenine can be addedInhibiting IL-2 synthesis, shortening the formation cycle of activated T lymphocytes, increasing the survival rate of individuals with high immune response, and inhibiting macrophages and T-lymphocytes. In vitro experiments can reduce prostaglandin E of mouse macrophage₂Leukotriene C₄Synthesis and inhibition of nitric oxide production. The sinomenine has obvious effect of eliminating formaldehyde arthritis and egg yolk arthritis of rats, preventing and stopping active anaphylactic shock of mice and treating adjuvant arthritis of rats. In addition, the pentacyclic triterpenoid in the tinea pedis flowers also has good anti-inflammatory and antibacterial effects.

The honeysuckle compound Chinese medicinal preparation consists of nine Chinese medicinal herbs, and has good antimicrobial, anti-inflammatory and trauma repair effects among the Chinese medicinal herbs. The Chinese herbal medicine flos Lonicerae is dried flower bud or flower with initial blossom of Lonicera japonica Thunb of Lonicera of Caprifoliaceae, and its effective components mainly include flavonoids, triterpenes, organic acids, etc. and volatile oil, and flos Lonicerae has antibacterial and antiviral effects: the antibacterial composition has obvious inhibition effects on staphylococcus aureus, hemolytic streptococcus, dysentery, typhoid fever, meningococcus, pneumococcus, pyocyanea, influenza virus and the like, and if the antibacterial composition is used with fructus forsythiae, the antibacterial ranges can be complemented; the honeysuckle has the function of enhancing immunity: the honeysuckle can promote the transformation of lymphocytes and enhance the phagocytic function of white blood cells. Diluting the honeysuckle decoction to 1: 1280 has effects in promoting phagocytic function of leukocyte. The honeysuckle injection injected into the abdominal cavity of the mouse also has the function of obviously promoting the phagocytic function of inflammatory cells; the honeysuckle has the anti-inflammatory function: the honeysuckle can promote the release of adrenocortical hormone and has obvious inhibition effect on inflammation. The injection of flos Lonicerae extract in abdominal cavity is 0.25g/kg, and can inhibit carrageenan foot swelling of rat. In addition, 30-40g/kg of honeysuckle injection is reported to reduce the degree of egg white edema. The injection of the honeysuckle extract solution into the abdominal cavity for 8g/kg for 2 times/day for 6 consecutive days also has obvious anti-exudation and anti-hyperplasia effects on oily meat bud sacs of croton in rats. Phillyrin is the main chemical component contained in Chinese herbal medicine forsythia, and mainly comprises two components of phillyrin and forsythiaside. The forsythia has the function of resisting pathogenic microorganisms: fructus forsythiae has inhibitory action on various gram positive and negative bacteria, the condensed decoction can inhibit typhoid bacillus, paratyphoid bacillus, colon bacillus, dysentery bacillus, diphtheria bacillus, cholera vibrio, staphylococcus, streptococcus, etc. in vitro, the inhibitory action of fructus forsythiae in vitro is similar to that of honeysuckle, the condensed decoction is the main antibacterial component in Yinqiao powder, the inhibitory action of honeysuckle on salmonella, especially typhoid bacillus and hemolytic streptococcus is similar to that of fructus forsythiae, the inhibitory action on dysentery bacillus and staphylococcus aureus is similar to that of fructus forsythiae, and the traditional Chinese medicine fructus forsythiae has anti-inflammatory action: the 50% forsythia alcohol extract aqueous solution 20ml/kg is injected into an abdominal cavity, has very obvious anti-exudation effect on rat croton oily bud sacs and the effect of reducing inflammatory focus capillary wall brittleness, can promote the formation of inflammatory barriers, is injected into the abdominal cavity by 30-40g/kg of 300% forsythia injection, has obvious inhibition effect on rat egg white foot swelling, and can also promote the phagocytosis effect on mouse inflammatory cells. The Chinese herbal medicine scutellaria contains baicalin, baicalein, wogonin, B-sitosterol, wogonoside, baicalein and the like, and scutellaria baicalensis has an antibacterial effect: in vitro tests show that the scutellaria baicalensis has strong antibacterial effect on dysentery bacillus, typhoid bacillus, pseudomonas aeruginosa, staphylococcus, hemolytic streptococcus, pneumococcus and the like; the scutellaria has the antiviral effect: scutellariae radix has effect in inhibiting influenza A virus PR 3; moreover, the scutellaria baicalensis can have a certain treatment effect after animals (mice) are infected with influenza; the scutellaria has antifungal effect: in vitro test the Scutellariae radix decoction has different degrees of inhibitory effect on various dermatophytes (such as epidermophyton inguinale). The Chinese herbal medicine phellodendron bark mainly contains various alkaloids such as berberine, palmatine and phellodendrine, and the phellodendron bark has broad-spectrum antibacterial action, has strong inhibiting effect on various dysentery bacilli and has synergistic action with the antibacterial activity of coptis and phellodendron bark; has strong inhibiting and killing effects on tubercle bacillus and leptospira; has obvious inhibiting effect on various fungi and trichomonad; cortex Phellodendri can be used for treating pyocutaneous disease, pyogenic infection, eczema and pruritus; cortex Phellodendri has trichomonad-resisting effect, and 10% concentration cortex Phellodendri decoction is mixed with trichomonad liquid 1:1, mixed culture, which has the inhibiting effect on trichomonas vaginalis; cortex Phellodendri has anti-hepatitis effect, and 6.25-100% cortex Phellodendri decoction has effect in inhibiting hepatitis B antigen, and phellodendrine has certain effect on chronic hepatitis; cortex Phellodendri has effect on immunity, and 100% concentration cortex Phellodendri decoction is administered by intragastric administration of 0.3ml for 7 days to increase the number of cells forming splenic plaque of mouse; cortex Phellodendri has antiulcer effect, and cortex Phellodendri extract (with berberine removed) 100mg/kg is administered by subcutaneous injection, or by intragastric injection or intradermal injection at 100, 1000mg/kg, and has inhibitory effect on gastric ulcer of rat induced by ethanol, aspirin or pylorus ligation. The Chinese herbal medicine houttuynia cordata contains decanoyl acetaldehyde, decanol, lauraldehyde, methyl nonanone and other components, wherein decanoyl acetaldehyde has the activity of resisting pathogenic microorganisms, and the houttuynia cordata has the effect of resisting pathogenic microorganisms and can inhibit staphylococcus aureus, bacillus influenzae and diplococcus pneumoniae; the houttuynia cordata has the function of enhancing the immune system and improving the phagocytosis of white blood cells of patients with chronic tracheitis; herba Houttuyniae has anti-tumor effect, and can improve CAMP in cancer cell to inhibit ehrlich ascites carcinoma; yu xing Cao can be used for treating sores and ulcers due to heat-toxicity, and often combined with heat-clearing and toxicity-removing herbs such as Zi Hua Di Ding, jin Yin Hua and Lian Qiao, etc., both for oral and external use. The Chinese herbal medicine radix Isatidis is root of Isatis tinctoria L and radix Isatidis belonging to Cruciferae, and root and rhizome of Strobilanthes cusia L belonging to Acanthaceae. Radix Isatidis has wide spectrum of antibacterial effect; the radix Isatidis has inhibitory effect on various pathogenic microorganisms such as virus, leptospira, fungi, etc.; radix Isatidis can be used for treating upper respiratory infection, pharyngolaryngitis, herpes zoster, epidemic pinkeye, viral encephalitis, tonsillitis, mumps, stomatitis, oral ulcer, and other infectious diseases. The Chinese herbal medicine rhizoma atractylodis is perennial herb of the genus atractylodis of the family compositae, the rhizoma atractylodis contains chemical components such as terpenoids, flavones, polyalkeneynes, volatile oil and the like, and the rhizoma atractylodis has the bacteriostasis function: the rhizoma Atractylodis extract has the effect of eliminating drug-resistant Shigella flexneri R plasmid, and can reduce the generation of drug resistance of bacteria. Soaking rhizoma Atractylodis in 95% ethanol for 10 hr, taking out rhizoma Atractylodis, placing on the ground of operating room to be sterilized, igniting until the rhizoma Atractylodis turns into ash, and obviously reducing bacterial colony number in air after sterilization compared with that before sterilization; the rhizoma atractylodis has the anti-tumor effect: the rhizoma atractylodis volatile oil, the atractylis lancea alcohol and the beta-eucalyptol l00mg/ml have the inhibition effect on esophageal cancer cells in vitro, wherein the atractylis alcohol has stronger action: the rhizoma atractylodis has stronger anti-ulcer effect: experiments show that the atractylis lancea and the rhizoma atractylodis north have strong inhibition effects on pylorus ligation-type ulcer, pylorus ligation-aspirin ulcer and stress ulcer, and can obviously inhibit ulcer, gastric juice amount, total acidity, total digestive ability and gastric mucosa damage of animals. The Chinese herbal medicine licorice is a perennial herb of leguminous. Has various medicinal effects, and more than 100 flavonoid compounds, more than 60 triterpenoid compounds, coumarins, 18 amino acids, various alkaloids, estrogen, various organic acids, etc. are separated from liquorice at home and abroad. The liquorice has the function of resisting pathogenic microorganisms: glycyrrhizin can directly destroy virus cells in test tubes, and also has inhibitory effect on varicella and herpes zoster virus. The antiviral effect of glycyrrhizin has a certain relationship with the direct effect on virus particles, interferon induction and natural killer cell activity increase: the liquorice has the functions of clearing heat and removing toxicity: the liquorice is warm in nature, has the functions of clearing heat and relieving heat toxin and has certain functions of treating diseases such as toxicosis, pain, swelling, furunculosis and the like; the liquorice has the effects of harmonizing the medicines: licorice has the effect of , and licorice is used to keep medical properties slightly for a while, and naturally has the beneficial meanings, and also has the effect of prolonging the validity period of and pulling it; the liquorice has the functions of anti-inflammation and anti-allergic reaction: glycyrrhetinic acid has inhibitory effect on cotton ball granuloma, formaldehyde edema, tuberculin reaction, and subcutaneous granulomatosis of white rat. The ammonium glycyrrhetate and the sodium glycyrrhetinate can effectively influence the exudation period and the proliferation period of the subcutaneous granulomatous inflammation, and the anti-inflammatory strength of the ammonium glycyrrhetate is weaker than or close to that of cortisone; the liquorice has the detoxification function: experiments prove that the liquorice and various preparations thereof have certain detoxification effect on various drug poisoning, animal poisoning, bacterial poisoning and the like, can relieve the poisoning symptoms and reduce the death rate of poisoned animals.

The externally applied anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea pedis is mainly prepared by the synergy of the medicinal effective components in the tinea pedis preparation and the honeysuckle compound traditional Chinese medicine preparation, and the ethanol has the main functions of assisting the antimicrobial effect of the medicine, increasing the solubility of the effective components of the fat-soluble medicine, increasing the concentration of the effective components of the medicine and enabling the medicine to easily reach the focus part. The externally applied anti-inflammatory compound traditional Chinese medicine preparation for treating the tinea capitis mainly aims to increase the antimicrobial and anti-inflammatory action pedigree of the spray and promote the synergistic action of wound healing, so that the time and the labor are saved when the oral ulcer, the inflammation and the trauma of animals are clinically treated. Experiments prove that the honeysuckle compound traditional Chinese medicine preparation, the externally applied anti-inflammatory preparation for the tinea pedis flower and the externally applied anti-inflammatory spray for the tinea pedis flower have good treatment effects on ulcer, inflammation and trauma of oral cavity skin of animals, the effective rate is 100%, and the cure rate is 85.7-100%.

The innovation points of the invention are as follows:

1. the preparation method of the alopecia areata preparation takes an acid solution as a medicine effective component extracting solution, and the principle is that the medicine effective component alkaloid in the Chinese herbal medicine alopecia areata is converted into alkaloid hydrochloride by hydrochloric acid, so that the solubility of the medicine effective component in the alopecia areata in the extracting solution is greatly increased, and the problem of low content of the medicine effective component in the alopecia areata preparation is solved;

2. the fundamental reason for the unstable therapeutic effect of the formulations of tinea pedis is the narrow antimicrobial spectrum (spectrum) of the formulations of tinea pedis. Tests show that the external anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea cruris has wider antimicrobial range (spectrum), narrower antimicrobial range and stronger antimicrobial effect than the external anti-inflammatory preparation for treating the tinea cruris, so that the problem of unstable treatment effect of the external anti-inflammatory preparation for treating the tinea cruris is solved;

3. the preparation method of the preparation for treating the tinea pedis adopts 20% ethanol to replace an ethanol extraction method, so that the production cost of the preparation is reduced; the preparation method of the tinea capitis preparation adopts an acidic alcohol-water mixed extraction method, replaces two processes of a tinea capitis water extraction method and an alcohol extraction method, evaporates ethanol in the decocting extraction process of the tinea capitis medicine, saves the ethanol evaporation reflux process, simplifies the preparation process of the tinea capitis preparation, and solves the problem that the preparation process of the external preparation of the tinea capitis is relatively complex;

4. due to the particularity of treating the external skin inflammation, ulcer and trauma of the animal, such as the safety of treatment workers, the treatment difficulty of parts such as the inner part of an animal oral cavity and the like, the difficulty in fixing medicines of parts such as animal hooves and the like, the population of the external skin inflammation, ulcer and trauma treatment of the animal, and the like, different dosage forms are prepared and used for treating the external skin inflammation, ulcer and trauma of the animal according to the external skin inflammation, ulcer and trauma diseases of different parts and different conditions of the animal, on the basis of ensuring the treatment effect, the treatment process is simplified, the treatment time is saved, the safety of treatment operators is ensured, and the problem of difficult operation of clinical treatment is solved.

Detailed Description

The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples are commercially available unless otherwise specified.

EXAMPLE 1 honeysuckle Compound Chinese medicinal preparation

The raw materials comprise: 60g of honeysuckle, 30g of rhizoma atractylodis, 60g of fructus forsythiae, 60g of scutellaria baicalensis, 60g of golden cypress, 60g of isatis root, 30g of houttuynia cordata and 30g of liquorice.

EXAMPLE 2 honeysuckle Compound Chinese medicinal preparation

The raw materials comprise: 70g of honeysuckle, 50g of rhizoma atractylodis, 70g of fructus forsythiae, 70g of scutellaria baicalensis, 70g of golden cypress, 70g of isatis root, 50g of houttuynia cordata and 50g of liquorice.

EXAMPLE 3 honeysuckle Compound Chinese medicinal preparation

The raw materials comprise: 80g of honeysuckle, 60g of rhizoma atractylodis, 80g of fructus forsythiae, 80g of scutellaria baicalensis, 80g of golden cypress, 80g of isatis root, 60g of houttuynia cordata and 60g of liquorice.

EXAMPLE 4 preparation of Compound Chinese medicinal preparation of honeysuckle flower

(1) Accurately weighing the honeysuckle, the rhizoma atractylodis, the forsythia, the scutellaria baicalensis, the phellodendron, the isatis root, the houttuynia cordata and the liquorice by using an electronic balance according to the formula amount. Except that rhizoma Atractylodis is put into multiple multi-layer gauze bags and sealed and soaked in another container with 200ml of 95% alcohol, other medicines are all poured into a porcelain pot, 1600ml of redistilled water, 400ml of absolute ethyl alcohol (analytically pure), 10ml of benzyl alcohol (analytically pure) and 10ml of glycerol (analytically pure) are added into the pot, the pH value is adjusted to 5.0 by hydrochloric acid (analytically pure) after the mixture is uniformly stirred, and the mixture is sealed and soaked. Stirring was carried out every 30 minutes. The drug was thus soaked for 12 h.

(2) After the medicines are soaked, the rhizoma atractylodis medicine and the liquid medicine are mixed in a pot and stirred uniformly, the mixture is heated and boiled for 2 hours, when the temperature of the liquid medicine is reduced to 60 ℃, the liquid medicine in the pot is filtered for preservation at 4 ℃, and the precipitate is discarded.

(3) Repeating the steps (1) and (2) 4 times;

(4) discarding residues, mixing the obtained medicinal liquids for 4 times, heating and evaporating the medicinal liquids, concentrating to 500ml, cooling to room temperature, filtering, autoclaving to obtain flos Lonicerae compound Chinese medicinal preparation, and storing at 4 deg.C for use.

EXAMPLE 5 preparation of Compound Chinese medicinal preparation of honeysuckle flower

(1) Weighing honeysuckle, rhizoma atractylodis, fructus forsythiae, scutellaria baicalensis, golden cypress, radix isatidis, houttuynia cordata and liquorice according to the formula, pouring other medicines except the rhizoma atractylodis into a porcelain pot, adding 2000ml of double distilled water, 500ml of absolute ethyl alcohol, 15ml of benzyl alcohol and 15ml of glycerol into the pot, uniformly stirring, adjusting the pH value to 6.0 by using hydrochloric acid, and sealing and soaking; stirring once every 60 minutes; soaking rhizoma Atractylodis in 100% 95% ethanol in another container under sealed condition; soaking the medicines for 24h;

(2) after the medicines are soaked, mixing the rhizoma atractylodis medicine and the liquid medicine into a pot, uniformly stirring, heating and boiling for 1.5 hours, filtering the liquid medicine in the pot at 0 ℃ for storage after the temperature of the liquid medicine is reduced to 50 ℃, and removing the precipitate;

(3) repeating the steps (1) and (2) for 3 times;

(4) removing residues, mixing the above medicinal liquids, heating and evaporating the medicinal liquids, concentrating to 250ml, cooling to room temperature, filtering, autoclaving to obtain the final product, and storing at 0 deg.C.

EXAMPLE 6 preparation of Compound Chinese medicinal preparation of honeysuckle flower

(1) Accurately weighing the honeysuckle, the rhizoma atractylodis, the forsythia, the scutellaria baicalensis, the phellodendron, the isatis root, the houttuynia cordata and the liquorice by using an electronic balance according to the formula amount. Except rhizoma Atractylodis is packed into multiple multilayer gauze bags and soaked in 200ml 95% ethanol in another container under sealed condition, other medicines are poured into a porcelain pot, 1600ml of redistilled water, 400ml of absolute ethyl alcohol (analytically pure), 5ml of benzyl alcohol (analytically pure) and 5ml of glycerol (analytically pure) are added into the pot, after stirring uniformly, the pH value is adjusted to 5.0 by hydrochloric acid (analytically pure), and the pot is soaked under sealed condition. Stirring was carried out every 30 minutes. The drug was thus soaked for 12 h.

(3) Repeating the steps (1) and (2) for 5 times;

(4) discarding residues, mixing the medicinal liquids obtained 5 times, heating and evaporating the medicinal liquids, concentrating to 500ml, cooling to room temperature, filtering, autoclaving to obtain flos Lonicerae compound Chinese medicinal preparation, and storing at 4 deg.C for use.

EXAMPLE 7 preparation of honeysuckle Compound traditional Chinese medicine anti-inflammatory granule and spray for external use

Accurately weighing 400ml of the honeysuckle compound traditional Chinese medicine preparation, adding 100ml of ethanol, uniformly stirring to obtain the honeysuckle compound traditional Chinese medicine preparation, and filling the honeysuckle compound traditional Chinese medicine preparation into a medicine spray can or a sprayer for flushing or spraying animal trauma.

EXAMPLE 8 preparation of honeysuckle Compound traditional Chinese medicine anti-inflammatory granule and spray for external use

Accurately weighing 800ml of the honeysuckle compound traditional Chinese medicine preparation, adding 200ml of ethanol, uniformly stirring to obtain the honeysuckle compound traditional Chinese medicine preparation, and filling the honeysuckle compound traditional Chinese medicine preparation into a medicine spray can or a sprayer for flushing or spraying animal trauma.

EXAMPLE 9 preparation of Compound traditional Chinese medicine anti-inflammatory powder with honeysuckle flower for external use

Accurately weighing 200ml of honeysuckle compound traditional Chinese medicine preparation and 100g of medical starch; mixing the above two at room temperature, air drying, and grinding to obtain topical Chinese medicinal powder.

EXAMPLE 10 preparation of Compound traditional Chinese medicine anti-inflammatory powder with honeysuckle flower for external use

Accurately weighing 400ml of honeysuckle compound traditional Chinese medicine preparation and 200g of medical starch; mixing the above two at room temperature, air drying, and grinding to obtain topical Chinese medicinal powder.

EXAMPLE 11 preparation of honeysuckle Compound traditional Chinese medicine external anti-inflammatory liniment

Accurately weighing 800ml of honeysuckle compound traditional Chinese medicine preparation, heating and evaporating the honeysuckle compound traditional Chinese medicine preparation into paste; mixing with 50ml of medical glycerin at room temperature to obtain the honeysuckle compound traditional Chinese medicine external anti-inflammatory liniment.

EXAMPLE 12 preparation of honeysuckle Compound traditional Chinese medicine external anti-inflammatory liniment

Accurately weighing 400ml of honeysuckle compound traditional Chinese medicine preparation, heating and evaporating the honeysuckle compound traditional Chinese medicine preparation into paste; mixing with 100ml medical glycerin at room temperature to obtain the honeysuckle compound traditional Chinese medicine external anti-inflammatory liniment.

EXAMPLE 13 preparation of a formulation of Ixeris pseudocerana

(1) Accurately weighing 500g of the dry powder of the tinea pedis flowers by using an electronic balance, filling the dry powder into a plurality of multi-layer gauze bags, tightening the bag openings, putting the bag openings into a porcelain pot, adding 1600ml of redistilled water and 400ml of absolute ethyl alcohol (analytically pure), uniformly stirring, adjusting the pH value to 2.0 by using hydrochloric acid (analytically pure), and sealing and soaking. Each pouch was squeezed thoroughly every 30 minutes. Thus soaking the medicine bag for 24 h.

(2) Extruding the liquid medicine in the gauze bag into a pot, filtering the liquid medicine and removing the precipitate; placing the filtered liquid medicine and the medicine bag in a pot, heating and boiling for 2h, screwing the liquid medicine in the gauze medicine bag into the pot when the temperature of the liquid medicine is reduced to 25 ℃, filtering the liquid medicine in the pot and removing the precipitate; then adjusting the pH value of the liquid medicine to 11.0 by using 1N NaOH, and filtering and precipitating for later use; heating the medicinal liquid to boil for 2 hr, filtering the medicinal liquid while heating, removing the precipitate, adjusting pH of the medicinal liquid to 2.0 with 1N hydrochloric acid, and filtering the precipitate; the liquid medicine is poured out at 4 ℃ and stored for later use.

(3) And (3) placing the medicine bag in a pot, repeating the steps (1) and (2) of soaking, boiling and adjusting the pH value, pouring out the liquid medicine at 4 ℃ and storing for later use. This was repeated 5 times.

(4) Discarding the medicated bag, mixing the obtained medicinal liquids for 5 times, adding 50ml of benzyl alcohol and 50ml of glycerol, adjusting pH of the medicinal liquid to 5.0 with 1N NaOH (analytically pure), heating and evaporating the medicinal liquid, concentrating to 500ml, cooling the medicinal liquid to room temperature, mixing with the above medicinal precipitate to obtain suspension, autoclaving to obtain the topical Chinese medicinal preparation, and storing at 4 deg.C.

EXAMPLE 14 preparation of a formulation of Ixeris pseudocerana

(1) Accurately weighing 1000g of the dry powder of the tinea pedis flowers by using an electronic balance, putting the powder into a plurality of multi-layer gauze bags, tightening the bag openings, putting the bag openings into a porcelain pot, adding 2000ml of redistilled water and 500ml of absolute ethyl alcohol (analytically pure), uniformly stirring, adjusting the pH value to 3.0 by using hydrochloric acid (analytically pure), and sealing and soaking. Each pouch was squeezed thoroughly every 30 minutes. Thus soaking the medicine bag for 12 h.

(2) Extruding the liquid medicine in the gauze bag into a pot, filtering the liquid medicine and removing the precipitate; placing the filtered liquid medicine and the medicine bag in a pot, heating and boiling for 1.5h, screwing the liquid medicine in the gauze medicine bag into the pot when the temperature of the liquid medicine is reduced to 20 ℃, filtering the liquid medicine in the pot and removing the precipitate; then NaOH of 1N is used for adjusting the pH value of the liquid medicine to 10.0, and the precipitate is filtered for standby; heating the medicinal liquid to boil for 1.5h, filtering the medicinal liquid while heating, removing the precipitate, adjusting pH of the medicinal liquid to 3.0 with 1N hydrochloric acid, and filtering the precipitate for use; pouring out the liquid medicine at 0 ℃ and storing for later use.

(3) And (3) placing the medicine bag in a pot, repeating the steps (1) and (2) of soaking, boiling and adjusting the pH value, pouring out the liquid medicine at 4 ℃ and storing for later use. This was repeated 3 times.

(4) Discarding the medicated bag, mixing the obtained medicinal liquids for 3 times, adding 150ml benzyl alcohol and 150ml glycerol, adjusting pH to 6.0 with 1N NaOH (analytically pure), heating and evaporating the medicinal liquid, concentrating to 1000ml, cooling to room temperature, mixing with the above medicinal precipitate to obtain suspension, autoclaving to obtain topical Chinese medicinal preparation, and storing at 4 deg.C.

Example 15 preparation of externally applied anti-inflammatory granule or spray of traditional Chinese medicine for treating tinea pedis

Accurately weighing 400ml of the preparation, adding 100ml of ethanol, stirring and mixing to obtain the preparation, and filling into a medicine spray can or a sprayer for washing or spraying animal trauma.

Example 16 preparation of externally applied anti-inflammatory granule or spray of traditional Chinese medicine for treating tinea pedis

Accurately weighing 800ml of the tinea pedis preparation, adding 200ml of ethanol, stirring and mixing to obtain the tinea pedis preparation, and filling into a medicine spray can or a sprayer for washing or spraying animal trauma.

Example 17 preparation of externally applied anti-inflammatory powder of a traditional Chinese medicine for treating tinea pedis

Accurately weighing 200ml of the tinea pedis preparation and 100g of medical starch; mixing the above two at room temperature, air drying, and grinding to obtain topical powder.

EXAMPLE 18 preparation of externally applied anti-inflammatory Chinese medicinal paint for treating tinea pedis

Accurately weighing 400ml of the tinea pedis preparation, and heating and evaporating the tinea pedis preparation into paste; mixing with 50ml of medical glycerin at room temperature to obtain the externally-applied anti-inflammatory liniment for treating alopecia.

EXAMPLE 19 Compound traditional Chinese medicinal preparation for external use for treating tinea pedis

The raw materials comprise: 200g of the concentrated ointment of the alopecia areata preparation in example 13, 200g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 100ml of ethanol.

EXAMPLE 20 externally applied anti-inflammatory preparation of Compound traditional Chinese medicine for treating tinea pedis

The raw materials comprise: 300g of the concentrated ointment of the alopecia areata preparation in example 13, 100g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 200ml of ethanol.

EXAMPLE 21 Compound traditional Chinese medicine preparation for external use for treating tinea pedis

The raw materials comprise: 100g of the concentrated ointment of the alopecia areata preparation in example 13, 300g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 100ml of ethanol.

EXAMPLE 22 Compound traditional Chinese medicinal preparation for external use for treating tinea pedis

The raw materials comprise: 300g of the concentrated ointment of the alopecia areata preparation in example 13, 300g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 50ml of glycerol.

EXAMPLE 23 Compound traditional Chinese medicine preparation for external use for treating tinea pedis

The raw materials comprise: 200g of the concentrated ointment of the alopecia areata preparation in example 13, 200g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 100ml of medical starch.

EXAMPLE 24 Compound traditional Chinese medicine preparation for external use for treating tinea pedis

The raw materials comprise: 100g of the concentrated ointment of the alopecia areata preparation in example 13, 100g of the concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation in example 2 and 200ml of medical starch.

EXAMPLE 25 preparation of Compound traditional Chinese medicine for external use anti-inflammatory preparation for treating tinea pedis

(1) Respectively adding 100ml of sterilized double distilled water into the tinea capitis preparation and the honeysuckle compound traditional Chinese medicine preparation according to the formula amount for dissolving, mixing and heating to 75-70 ℃, and stirring;

(2) when the temperature is reduced to 25 ℃, adding auxiliary materials, stirring and uniformly mixing to form the externally-applied anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea pedis, and filling the externally-applied anti-inflammatory preparation into a medicine spray can or a sprayer for washing or spraying animal trauma (granules or sprays).

EXAMPLE 26 preparation of Compound traditional Chinese medicine anti-inflammatory powder for external use for treating tinea pedis

Accurately weighing 200g of a concentrated ointment of the tinea pedis flower preparation, 200g of a concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation and medical starch; and (3) respectively adding 100ml of sterilized double distilled water into the tinea capitis preparation and the honeysuckle compound traditional Chinese medicine preparation for dissolving, uniformly mixing with 200g of medical starch, air-drying, and grinding to obtain the tinea capitis compound traditional Chinese medicine external anti-inflammatory powder.

EXAMPLE 27 preparation of Compound traditional Chinese medicinal anti-inflammatory liniment for external use for treating tinea pedis

Accurately weighing 200g of a concentrated ointment of the tinea pedis flower preparation and 200g of a concentrated ointment of the honeysuckle compound traditional Chinese medicine preparation; mixing with 50ml of medical glycerin at room temperature to obtain the externally applied anti-inflammatory liniment of the compound traditional Chinese medicine for treating the tinea pedis.

Effect test

The culture temperature in the test is 24 ℃ suitable for the culture of yeast and 37 ℃ suitable for the culture of other bacteria.

1. Experimental part

1.1 screening test of inhibiting microorganism for different Chinese herbal medicine preparations

1.1.1 screening preliminary test for inhibiting microorganisms of different Chinese herbal medicine preparations

1.1.1.1 interaction screening preliminary test for Forsythia suspensa, houttuynia cordata, Scutellaria baicalensis and phellodendron amurense

1.1.1.1.1 level factor chart

The interaction level factors of forsythia, houttuynia cordata, scutellaria baicalensis and phellodendron amurense are shown in table 1.

1.1.1.1.2 preparation:

the preparation was carried out as described in example 4, and finally the different preparations were concentrated by evaporation 1-fold to give the experimental preparation.

1.1.1.1.3 Experimental methods:

using orthogonal empirical table L₁₆(4⁵) Different preparations are prepared for horizontal factor combination, after the salmonella paratyphi for experiment is purified, the purified salmonella paratyphi is inoculated into THB nutrient broth, and after the salmonella paratyphi is cultured for 24 hours at 37 ℃ or 24 ℃, the salmonella paratyphi and the THB nutrient broth are respectively counted by a plate counting method to prepare 10000 cfu/ml strain suspension; mu.l of the strain suspension was added to 6ml of each culture broth prepared by mixing the preparation and the THB nutrient broth at a ratio of 1:1, and the mixture was cultured at 37 ℃ for 16 hours and then counted by plate counting. And finally calculating the inhibition rate.

1.1.1.1.4 results and discussion of the experiments

The results of the interaction experiments of forsythia, houttuynia cordata, scutellaria baicalensis and phellodendron amurense are shown in table 2.

The interaction experiment result of forsythia, houttuynia cordata, scutellaria baicalensis and phellodendron amurense shows that: in terms of factors, fructus forsythiae is larger than radix scutellariae, golden cypress is larger than herba houttuyniae; in terms of level, the 4 th level of forsythia is the best, the 2 nd level of scutellaria is the best, the 3 rd level of phellodendron is the best and the 3 rd level of houttuynia is the best. According to the principle of orthogonal experiments, 50g of fructus forsythiae, 25g of houttuynia cordata, 37.5g of scutellaria baicalensis and 37.5g of golden cypress are selected as the proportion basis for further carrying out the honeysuckle compound traditional Chinese medicine preparation.

1.1.1.2 interaction screening preliminary test for honeysuckle compound traditional Chinese medicine preparation

1.1.1.2.1 level factor chart

The interaction level factor table of honeysuckle, isatis root, licorice and atractylodes is shown in table 3.

1.1.1.2.2 preparation:

the preparation was carried out as described in example 4.

1.1.1.2.3 Experimental methods:

based on the interaction experiment of fructus forsythiae, herba Houttuyniae, Scutellariae radix and cortex Phellodendri, 50g fructus forsythiae, 25g herba Houttuyniae, 37.5g Scutellariae radix and 37.5g cortex Phellodendri are used as basic drugs, and the above orthogonal experiment table L is used₁₆(4⁵) Different preparations are prepared for horizontal factor combination, after the salmonella paratyphi for experiment is purified, the purified salmonella paratyphi is inoculated into THB nutrient broth, and after the salmonella paratyphi is cultured for 24 hours at 37 ℃ or 24 ℃, the salmonella paratyphi and the THB nutrient broth are respectively counted by a plate counting method to prepare 10000 cfu/ml strain suspension; mu.l of the strain suspension was added to 6ml of each culture broth prepared by mixing the preparation and the THB nutrient broth at a ratio of 1:1, and the mixture was cultured at 37 ℃ for 16 hours and then counted by plate counting. And finally calculating the inhibition rate.

1.1.1.2.4 results and discussion of the experiments

The interaction experiment results of the honeysuckle compound traditional Chinese medicine preparation are shown in table 4.

The interaction experiment result of the honeysuckle, the isatis root, the liquorice and the rhizoma atractylodis in the honeysuckle compound traditional Chinese medicine preparation shows that: in terms of factors, honeysuckle flower, isatis root, liquorice and rhizoma atractylodis; in terms of level, the 4 th level of honeysuckle is the best, the 4 th level of isatis root is the best, the 3 rd level of liquorice is the best, and the 3 rd level of rhizoma atractylodis is the best but not much different from the 2 nd level. According to the principle of orthogonal experiments, the interaction experiment result of fructus forsythiae, herba houttuyniae, radix scutellariae and cortex phellodendri and the interaction experiment result of honeysuckle, radix isatidis, liquorice and rhizoma atractylodis are combined, and the formula in claim 1 is screened out as the basic proportion formula of the honeysuckle compound traditional Chinese medicine preparation.

1.1.1.3 screening and pre-testing interaction of compound traditional Chinese medicine external preparation for treating tinea capitis

1.1.1.3.1 preparation:

the honeysuckle compound traditional Chinese medicine preparation is prepared according to the method in the embodiment 4, the tinea pedis preparation is prepared according to the method in the embodiment 13, and finally different preparations are evaporated, concentrated into paste and dried in the air to obtain the experimental medicine.

1.1.1.3.2 Experimental methods:

the following table shows the preparation of different formulations, which were prepared into 400ml aqueous solution for experiments. Purifying salmonella paratyphi for experiments, inoculating the purified salmonella paratyphi into THB nutrient broth, culturing the purified salmonella paratyphi at 37 ℃ or 24 ℃ for 24 hours, and respectively counting the salmonella paratyphi by using a plate counting method to prepare 10000 cfu/ml strain suspension; mu.l of the strain suspension was added to 6ml of each culture broth prepared by mixing the preparation and the THB nutrient broth at a ratio of 1:1, and the mixture was cultured at 37 ℃ for 16 hours and then counted by plate counting. And finally calculating the inhibition rate.

1.1.1.3.2 results and discussion of the experiments

The results of the interaction experiments of the external Chinese medicinal preparations are shown in Table 5.

In order to ensure that the experiment result is obvious, the application concentration is diluted by 2 times in the experiment, and although the bacteriostasis rate is low, the difference between the bacteriostasis rates of all the formulas can be more clearly seen. From the interaction experimental results of the traditional Chinese medicine external preparation, the effect of inhibiting salmonella paratyphi of the compound traditional Chinese medicine external preparation for treating alopecia areata (the compound external preparation for treating alopecia areata and the compound traditional Chinese medicine preparation for honeysuckle) is better than that of the independent preparation for treating alopecia areata and the independent compound external preparation for honeysuckle; the formula 4 of the compound traditional Chinese medicine external preparation for treating tinea capitis has the best effect of inhibiting salmonella paratyphi, so the formula 4 is preferably a basic proportion formula of the compound traditional Chinese medicine external preparation for treating tinea capitis.

1.1.2 screening test of inhibitory microorganisms for different formulations of Chinese herbal medicine

1.1.2.1 the formula comprises: the herbal components of the different formulations are shown in table 6.

1.1.2.2 formulation of different formulations:

the formulations of preparation I, preparation II and preparation III were the formulations of example 1, example 2 and example 3, respectively, and the preparation process was carried out in the same manner as in example 4; formulation IV was prepared as described in example 13; formulation v, formulation vi and vii were formulated as sprays as described in example 25.

1.1.2.3 Experimental methods:

purifying different microbial strains for experiments, inoculating the strains into corresponding THB nutrient broth or yeast nutrient broth, culturing at 37 ℃ or 24 ℃ for 24h, and respectively counting by using a plate counting method to prepare 10000 cfu/ml microbial suspension; mu.l of each microorganism suspension was added to 6ml of a culture broth prepared from each preparation and the corresponding nutrient broth at a ratio of 1:1, and the mixture was cultured at 37 ℃ or 24 ℃ for 16 hours and then counted by a plate counting method. And finally calculating the inhibition rate.

1.1.2.4 results of the experiment: the bacteriostasis rates of the preparations to different microorganisms are shown in table 7, and the different microorganisms are respectively saccharomycetes, staphylococcus aureus, hemolytic streptococcus, streptococcus agalactiae, O2 escherichia coli, K88 escherichia coli, salmonella paratyphi and pasteurella.

1.1.2.5 discuss:

the honeysuckle compound traditional Chinese medicine preparation (preparation I, preparation II and preparation III) and the external anti-inflammatory preparation for treating alopecia (preparation VI) have good antimicrobial effect, but the spectrum of the effect is different. The externally applied anti-inflammatory spray (preparation VII) of the compound traditional Chinese medicine of the tinea capitis can increase the antimicrobial effect of the preparation and has wider antimicrobial spectrum. Therefore, the honeysuckle compound traditional Chinese medicine preparation, the externally applied anti-inflammatory preparation for treating the tinea capitis and the externally applied anti-inflammatory spray for treating the tinea capitis are screened out according to experimental results, and the externally applied anti-inflammatory spray for treating the inflammation, the ulcer and the trauma of the animal skin are three Chinese herbal medicine externally applied anti-inflammatory preparations.

According to the experimental results, the preparation I with the lowest traditional Chinese medicine amount in the honeysuckle compound traditional Chinese medicine preparation is taken as a representative formula of the honeysuckle compound traditional Chinese medicine preparation; the preparation V with the lowest antimicrobial effect in the compound traditional Chinese medicine external anti-inflammatory spray for treating the tinea pedis is taken as a representative formula of the compound traditional Chinese medicine external anti-inflammatory spray for treating the tinea pedis, and the following subsequent pharmacodynamic experiments are carried out. Taking a preparation III with the highest traditional Chinese medicine amount in the honeysuckle compound traditional Chinese medicine preparation as a representative formula of the honeysuckle compound traditional Chinese medicine preparation; the skin irritation experiment of the following rabbits was performed by using a preparation VI in the compound traditional Chinese medicine external anti-inflammatory spray for treating tinea capitis as a representative formula of the compound traditional Chinese medicine external anti-inflammatory spray for treating tinea capitis.

1.2 inactivation test of ORF virus by three Chinese herbal medicine external anti-inflammatory preparations

1.2.1 materials and methods

Materials: the honeysuckle compound traditional Chinese medicine preparation, the tinea capitis external preparation and the tinea capitis compound traditional Chinese medicine external preparation are prepared according to the methods of example 4, example 13 and example 25 respectively; the Orf virus is a HCE strain preserved by the livestock veterinary institute in kansu province (a HCE strain preservation unit specified by central authorities in the livestock veterinary institute in kansu province); the experimental goat is a healthy lamb of 3-6 months old provided by a white temple country farmer in a mountain Kongtong area in PingLian city of Gansu province.

HCE strain treatment: accurately weighing 200mg HCE scab venom with a balance, grinding the scab venom with 20ml Hank's solution containing 1000 units/ml, acting at 4 deg.C for 24 hr, freeze thawing for 3 times, centrifuging at 3000 r/min, collecting supernatant, and storing at-80 deg.C. 6h before sheep body challenge, the thawed venom was diluted 10-fold to 10-fold with different drugs (experimental group) or Hank's solution (control group)^-1、10^-2、10^-3、10^-4、10^-5And acting at 4 ℃ for later use.

The sheep body toxin counteracting method comprises the following steps: after ear number punching, 60 experimental sheep were randomly divided into 4 groups, 10 groups were used for each group^-1、10^-2、10^-3、10^-4、10^-5Inoculating 0.2ml of the doubled venom to the experimental sheep by oral scratching, and repeating the concentration of each venom for 3 times; the results were observed 7d-10d after inoculation.

1.2.2 results

The control group has 15 sheep inoculated with the feed for 7d to 10d, and has different degrees of oral mucosa red swelling, ulcer and abscess; experimental group 45 sheep with tinea capitis and tinea versicolorExperimental group 10 with anti-inflammatory agent^-1The concentration of the venom is that 1 sheep has red and swollen oral mucosa, and the rest 44 experimental sheep have no disease symptoms. Experimental results show that the honeysuckle compound traditional Chinese medicine preparation, the externally applied anti-inflammatory preparation for the tinea capitis and the externally applied anti-inflammatory spray for the tinea capitis have good inactivation effect on ORF viruses.

1.3 Rabbit skin irritation test

Animal grouping and pretreatment in experiment: 20 healthy white rabbits with the weight of 2.0-2.5 kg and the weight of half of the male and female animals are taken and bred for 5 days before the experiment, and the rabbits are randomly divided into 4 groups (a control group 1 and an experiment group 3) according to the sex and the weight if the rabbits are normal, and each group comprises 5 rabbits. Shearing off hairs on two sides of the spine of the back of the rabbit 24h before administration of each group, and then depilating with a razor; the skin on the right side of the depilated area was scored with a sterile needle in the shape of a small "#" until the degree of bleeding was zero, and was used as a damaged skin group and the left side was an intact skin group.

Pretreatment of a pharmaceutical preparation experiment: respectively evaporating and concentrating the honeysuckle compound traditional Chinese medicine preparation, the externally applied anti-inflammatory preparation for treating tinea pedis and the externally applied anti-inflammatory preparation for treating tinea pedis prepared in the embodiments 1 to 3 into paste, and mixing the paste with 20 percent of sterilized glycerin for use; the control group was sterilized glycerin instead of ointment.

The administration method comprises the following steps: each experimental group of animals was coated with 2g of ointment on the right depilatory area, and the control group was coated with about 2g of glycerin as a control. After administration, the gauze and the preservative film are partially covered and fixed by adhesive tape, the gauze and the preservative film are removed after the medicine is kept in contact with the skin for 4h, and then the residual medicine is washed away by warm water. The administration was 1 time per day for 5 days. The skin condition was observed 1h after each drug removal and before re-administration. The presence or absence of erythema and edema and recovery at the site of application was observed and recorded again at 1, 24, 48 and 72 hours after the last administration and removal of the drug, and the evaluation criteria are shown in tables 8 and 9.

As a result: the experimental administration and observation show that the left side of each group of skin has no obvious skin irritation reaction symptoms such as erythema, edema and the like after 1h after each drug removal, before re-administration and 1, 24, 48 and 72h after the last administration; after the right-side skin is administrated, slight red and swollen scratch parts can be seen on the 1 st day of 3 experimental groups and control groups, the scratch parts completely recover to be normal until the 4 th day, and after 1h after the medicine is removed every time, before the medicine is administrated again and after 1, 24, 48 and 72h after the medicine is administrated last time, no obvious skin irritation reaction symptoms such as erythema, edema and the like exist through naked eye observation, and all scores are zero.

1.4 stability test of therapeutic Effect of Each preparation

The honeysuckle compound traditional Chinese medicine preparation, the tinea capitis external anti-inflammatory preparation and the tinea capitis compound traditional Chinese medicine external anti-inflammatory spray which are prepared by the embodiments of the invention are stored for 1 year at 4 ℃, and the clinical treatment effect is not changed; the compound traditional Chinese medicine external anti-inflammatory preparation for treating the tinea capitis prepared from the traditional Chinese medicines has no change in clinical treatment effect.

2. Clinical application

2.1 materials

2.1.1 test animals

The sheep is treated by the sheep raised in Gansu, Shaanxi and Ningxia.

2.1.2 formulations

Taking the honeysuckle compound traditional Chinese medicine preparation, the tinea pedis preparation and the tinea pedis compound traditional Chinese medicine external anti-inflammatory powder prepared by the embodiment of the invention. The herbal components of the different formulations are shown in table 6.

2.2 methods

2.2.1 treatment trials of the baldness preparation for surgical diseases in animals

For wounds of animals caused by various mechanical reasons or infected wounds, papules, abscesses and ulcers, cleaning and flushing the injured parts with sterile normal saline, dipping sterile dry absorbent cotton in the sterile normal saline, applying an anti-inflammatory preparation to the injured parts which are easy to fix and coat, fixing the injured parts with sand cloth and adhesive plaster for 1 time every day, and treating for 3-5 days; for the injured parts which are not easy to fix and coat, the anti-inflammatory spray is used for treating 3-5 days 2-3 times per day.

Hot compressing affected part of animal with scabies with warm water of about 60 deg.C for 10min, removing superficial cortex, applying topical Chinese medicinal preparation on the affected part, fixing with gauze and adhesive tape 1 time per day, and treating for 3-5 days; for the injured parts which are not easy to fix and coat, the externally applied anti-inflammatory spray of the compound traditional Chinese medicine for treating the tinea pedis is used for 2 to 3 times per day for 3 to 5 days.

For the fistula caused by animal infection, thoroughly washing and cleaning the interior of the fistula with sterile physiological saline and anti-inflammatory preparation, then plugging the traditional Chinese medicine external anti-inflammatory powder into the fistula, and finally fixing the fistula orifice with gauze and adhesive plaster 1 time per day for treatment for 3-5 days.

2.2.2 Orf therapeutic trials

Sheep and goats, which had developed Orf (contagious pustular dermatitis in sheep) and developed overt clinical symptoms, were tested for treatment with different topical anti-inflammatory formulations. For papules, blisters, pustules and ulcers caused by Orf, the papules, blisters, pustules and ulcer wounds need to be firstly scratched and cleaned, (the parts which form verrucous scab skin in the convalescent period are not generally treated.) the papules, blisters, pustules and ulcers are rinsed by normal saline, then dipped in sterile dry absorbent cotton for drying, and the external anti-inflammatory preparation is coated or sprayed on the papules, blisters, pustules and ulcerated parts for 2-3 times a day for treating 3-5 days. (for the wounded parts which are easy to fix and easy to coat, the external anti-inflammatory preparation can be coated on the wounded parts and fixed by a gauze and an adhesive tape 1 time a day for treating 3-5 d.) the diseased sheep can be recovered to be normal within 5d and cured.

2.3 results and discussion

2.3.1 results

2.3.1.1 therapeutic test results of external anti-inflammatory agent for animal surgical diseases

The results of the experimental treatment of surgical wounds, papules, abscesses, ulcers, fistulas and scabies in animals with the external anti-inflammatory preparation are shown in tables 10-11. As can be seen from the following table, the cure percentages of the preparation I, the preparation IV and the preparation V on the surgical wounds, the fistulae and the scabies of the animals are 94.3 percent, 95.1 percent and 98.1 percent respectively; 85.7, 85.7 and 87.5%; 94.7, 89.5 and 100.0%. The treatment test result shows that the external anti-inflammatory preparation has good treatment effect on animal surgical wounds, fistulae and mange.

2.3.1.2 therapeutic results of different preparations on Orf virus Orf

The results of different formulations of the bald sore flower formulation on Orf treatment are shown in the table above. As can be seen from the above table, the cure rates of the Chinese medicinal preparations I, IV and V on the Orf of the lambs and the adult sheep are respectively 87.6%, 91.5%, 92.1% and 89.2%, 93.3% and 95.0%. The results of Orf treatment experiments show that the external anti-inflammatory preparation has good treatment effect on Orf.

2.3.2 discussion

With the globalization of world trade, the introduction of new animal diseases and the complication of old diseases make the prevention and treatment of livestock and poultry diseases increasingly difficult, meanwhile, the number of zoonosis increases, the complexity thereof increases, and particularly the outbreak of diseases such as avian influenza and the like is related to animal diseases, so that the prevention and treatment difficulty of animal diseases is increased. The health degree of animals is not only related to the life quality of people, but also related to the safety of public health of society. Although the development of modern medicine, pharmacology and molecular biology provides various prevention and treatment methods for animal diseases, the problems of drug resistance and residues of antibiotics and the like in many aspects still cannot be solved in the face of complex and various animal diseases. However, the traditional Chinese herbal medicine in China has unique curative effect on many diseases which cannot be solved by modern medicine and is easy to be metabolized, converted and eliminated in animal bodies and external environment, and the utilization of the unique Chinese herbal medicine in China and the achievement obtained at present to serve animal husbandry and benefit human beings is the obligation and responsibility of veterinarians.

From the results of the test on the treatment of the surgical wounds, the scabies and the fistulas of the animals, it is known that the external anti-inflammatory preparation has a very good treatment effect on the surgical wounds, the scabies and the fistulas of the animals, and in the treatment process, it is found that the external anti-inflammatory preparation has a killing or inhibiting effect on pathogenic microorganisms and has a promoting effect on the healing of the surgical wounds, the scabies and the fistulas and the repair of tissues. From the result of the external anti-inflammatory preparation on the Orf treatment, the preparation V, the preparation VI and the preparation VII have good treatment effects on the Orf of the lambs and the adult sheep, and the preparation VII has the best treatment effect on the Orf.

Claims

1. A traditional Chinese medicine external anti-inflammatory preparation is characterized in that: the honeysuckle compound traditional Chinese medicine preparation is prepared from the following raw material medicines in parts by weight: 60-80 parts of honeysuckle, 30-60 parts of rhizoma atractylodis, 60-80 parts of fructus forsythiae, 60-80 parts of radix scutellariae, 60-80 parts of cortex phellodendri, 60-80 parts of radix isatidis, 30-60 parts of herba houttuyniae and 30-60 parts of liquorice.

2. A traditional Chinese medicine external anti-inflammatory preparation is characterized in that: the externally applied anti-inflammatory preparation is prepared from the following raw material medicines in parts by weight, 1-3 parts of a tinea capitis preparation, 1-3 parts of a honeysuckle compound traditional Chinese medicine preparation and an auxiliary material, wherein the auxiliary material is 1-2 parts of ethanol or 1-2 parts of glycerol or medical starch.

3. The method for preparing a topical anti-inflammatory Chinese medicinal preparation according to claim 1, comprising the steps of:

(2) after the medicines are soaked, mixing the rhizoma atractylodis soaking solution and other medicine soaking solutions, uniformly stirring the mixture in a pot, heating and boiling the mixture for 1.5 to 2 hours, filtering the medicine liquid in the pot at the temperature of between 0 and 4 ℃ for later use after the temperature of the medicine liquid is reduced to between 50 and 60 ℃, and removing the precipitate;

(3) repeating the step (1) and the step (2) for 3-5 times;

4. The method for preparing a topical anti-inflammatory Chinese medicinal preparation according to claim 3, wherein the preparation comprises the following steps: the preparation is a granule or a spray, and is prepared by adding 1-2 parts of ethanol into 4-8 parts of a honeysuckle compound traditional Chinese medicine preparation, and uniformly stirring.

5. The method for preparing a topical anti-inflammatory Chinese medicinal preparation according to claim 3, wherein the preparation comprises the following steps: the preparation is powder, 2-4 parts of honeysuckle compound traditional Chinese medicine preparation and 1-2 parts of medical starch, and the honeysuckle compound traditional Chinese medicine preparation and the medical starch are mixed uniformly at room temperature, air-dried and ground to obtain the preparation.

6. The method for preparing a topical anti-inflammatory Chinese medicinal preparation according to claim 3, wherein the preparation comprises the following steps: the preparation is a liniment, and the honeysuckle compound traditional Chinese medicine preparation is heated and evaporated into paste in 4-8 parts; mixing with 0.5-1 part of medical glycerin at room temperature.

7. The method for preparing a topical anti-inflammatory Chinese medicinal preparation of claim 2, wherein the preparation comprises the following steps: the preparation of the tinea pedis flower is prepared by the following steps,

(2) extruding the liquid medicine in the gauze medicine bag into a pot, filtering the liquid medicine and removing the precipitate; placing the filtered liquid medicine and the medicine bag in a pot, heating and boiling for 1.5-2h, screwing the liquid medicine in the gauze medicine bag into the pot when the temperature of the liquid medicine is reduced to 20-25 ℃, filtering the liquid medicine in the pot and removing the precipitate; then adjusting the pH value of the liquid medicine to 10.0-11.0 by NaOH, filtering and precipitating for later use; heating the medicinal liquid to boil for 1.5-2h, filtering the medicinal liquid while the medicinal liquid is hot, removing the precipitate, adjusting pH of the medicinal liquid to 2.0-3.0 with hydrochloric acid, and filtering the precipitate for later use; pouring out the medicinal liquid, and storing at 0-4 deg.C;

8. The method for preparing a topical anti-inflammatory Chinese medicinal preparation according to claim 7, wherein the preparation comprises the following steps: the preparation is a granule or a spray, and is prepared by adding 1-2 parts of ethanol into 4-8 parts of the preparation, and uniformly stirring.

9. The method for preparing a topical anti-inflammatory Chinese medicinal preparation of claim 2, wherein the preparation comprises the following steps: the preparation process of the externally applied anti-inflammatory preparation of the compound traditional Chinese medicine for treating the tinea pedis is as follows:

10. Use of a topical anti-inflammatory formulation of a Chinese medicinal material as claimed in claim 1 or claim 2 in the manufacture of a medicament for the treatment of ulcerations, inflammation and trauma in the oral skin of an animal.