CN110237157B

CN110237157B - Chinese herbal medicine preparation for treating influenza and preparation method thereof

Info

Publication number: CN110237157B
Application number: CN201910451109.XA
Authority: CN
Inventors: 颜健华; 罗廷荣; 李晓宁; 梁晶晶; 李献
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2019-05-28
Filing date: 2019-05-28
Publication date: 2021-08-10
Anticipated expiration: 2039-05-28
Also published as: CN110237157A

Abstract

The invention discloses a Chinese herbal medicine preparation for treating influenza and a preparation method thereof, belonging to the field of traditional Chinese medicines. The Chinese herbal medicine preparation consists of 10-15 parts of houttuynia cordata extract, 10-15 parts of scutellaria baicalensis extract, 10-15 parts of dandelion extract, 8-15 parts of rheum officinale, 5-10 parts of radix bupleuri, 5-10 parts of fructus forsythiae, 5-10 parts of bovine ears, 5-10 parts of clausena lansium, 8-15 parts of dayflower, 8-15 parts of oriental wormwood, 10-30 parts of honey and 1-5 parts of bamboo rat oil. The preparation method comprises decocting radix et rhizoma Rhei, bupleuri radix, fructus forsythiae, herba Arctii, cortex Phellodendri, herba Commelinae and herba Artemisiae Scopariae twice, mixing decoctions, adding herba Houttuyniae extract, herba Taraxaci extract and Scutellariae radix extract, mixing, adding Mel, stirring, mixing, kneading, and adding bamboo rat oil and pill. The invention has reasonable formula, simple preparation method, safety, no toxic or side effect, no drug residue, no drug resistance, high bioavailability and obvious effect on treating influenza.

Description

Chinese herbal medicine preparation for treating influenza and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicines, in particular to a Chinese herbal medicine preparation for treating influenza and a preparation method thereof.

Background

Influenza (influenza for short) is acute respiratory tract infection caused by influenza virus, is also a disease with strong infectivity and high transmission speed, is highly developed all over the world, particularly in autumn and winter, and has clinical symptoms of acute high fever, general pain, obvious hypodynamia and mild respiratory tract symptoms, and the caused complications and death phenomena are very serious. The disease is caused by influenza virus, can be divided into three types of A (A), B (B) and C (C), the A virus often has antigen variation, has large infectivity, is quickly spread, is very easy to have wide-range epidemics, and is mainly spread by droplets in the air, contact between people or contact with contaminated products, so the disease has the characteristics of strong infectivity, difficult control of a spreading path, high spreading speed and wide spreading range. Influenza is susceptible to pneumonia, myocarditis, septicemia, meningitis and the like, and can cause multiple organ failure and death in severe cases. According to statistics, the number of unnatural people died caused by influenza in China is about 20 ten thousand every year.

Chinese herbal medicine is the most traditional and naturally existing medicinal materials in China, the resource sources are extremely rich, the price is relatively low, and the Chinese herbal medicine contains various natural chemical components and nutritional components, such as tannin, saponin, alkaloid, organic acid, vitamins, mineral substances, trace elements and the like, the components have the effects of resisting bacteria, resisting viruses, resisting inflammation, clearing heat and the like, can improve the immune function, improve the production performance, supplement nutrition, have no side effect and the like, the Chinese herbal medicine preparation has certain advantages in treating influenza, particularly influenza virus is easy to generate drug resistance, and therefore, the development of drugs for treating influenza, which have good effects, no drug resistance, no side effect and are convenient to carry and take, is still expected by people.

Disclosure of Invention

The invention aims to solve the problems in the prior art and provides a Chinese herbal medicine preparation which has the advantages of reasonable formula, obvious curative effect, simple preparation method and no side effect and can effectively cure influenza.

The raw materials of the invention are all from Chinese herbal medicine, prepare a Chinese herbal medicine preparation for treating influenza scientifically, this Chinese herbal medicine preparation has effects such as antibiotic, antiviral, diminishing inflammation, clearing heat and improving organism immunity, etc., especially this pill is prepared from concentrated solution and extract preparation of the medicament, not merely have decoction easy to absorb, with instant effect characteristic, and also possess the lasting efficacy of pill, save the medicinal material, characteristic easy to carry and administrate, it is the fine medicine for treating influenza.

In order to realize the purpose of the invention, the adopted technical scheme is as follows:

a Chinese herbal medicine preparation for treating influenza is prepared from the following raw materials: houttuynia cordata extract, dandelion extract, scutellaria baicalensis extract, rheum officinale, radix bupleuri, fructus forsythiae, oxear, clausena lansium, dayflower, oriental wormwood, honey and bamboo rat oil.

Preferably, the composition consists of the following components in parts by weight: 10-15 parts of houttuynia cordata extract, 10-15 parts of scutellaria baicalensis extract, 10-15 parts of dandelion extract, 8-15 parts of rheum officinale, 5-10 parts of radix bupleuri, 5-10 parts of fructus forsythiae, 5-10 parts of oxear, 5-10 parts of clausena lansium, 8-15 parts of dayflower, 8-15 parts of oriental wormwood, 5-20 parts of honey and 1-5 parts of bamboo rat oil.

Preferably, the extraction method of the houttuynia cordata extract comprises the following steps: sun drying herba Houttuyniae, grinding into powder, adding organic solvent for extraction, and purifying the extractive solution;

the organic solvent is one or more of toluene, dichloromethane and butanol.

Preferably, the method for extracting the dandelion extract comprises the following steps: drying herba Taraxaci in the sun, grinding into powder, adding organic solvent for extraction, and purifying the extractive solution;

the organic solvent is one or more of toluene, dichloromethane and butanol.

Preferably, the extraction method of the scutellaria baicalensis extract comprises the following steps: drying Scutellariae radix in the sun, grinding into powder, extracting with organic solvent, and purifying the extractive solution;

the organic solvent is one or more of toluene, dichloromethane and butanol.

Preferably, the bamboo rat oil is obtained by reasonably slaughtering an artificially-bred Yinxing bamboo rat and then refining leaf fat of the artificially-bred Yinxing bamboo rat.

The invention also provides a preparation method of the Chinese herbal medicine preparation for treating influenza, which comprises the following steps:

(1) weighing the components according to the weight parts of the raw materials;

(2) adding radix et rhizoma Rhei, bupleuri radix, fructus forsythiae, herba Arctii, cortex Phellodendri, herba Commelinae and herba Artemisiae Scopariae into drinking water, soaking for 20-30 min, adding water to submerge the materials, decocting the casserole on fire, boiling for 40-50 min, and pouring out the medicinal liquid to obtain first medicinal liquid; adding warm water into the casserole, decocting for the second time, boiling for 30 min, pouring out the medicinal liquid, mixing with the first medicinal liquid, naturally cooling, decocting for two times, and mixing the decoctions;

(3) adding herba Houttuyniae extract, herba Taraxaci extract and Scutellariae radix extract, mixing, adding decocted Mel, stirring while pouring, kneading with food-grade gloves, and adding bamboo rat oil and pill.

The invention also provides a use method of the Chinese herbal medicine preparation for treating influenza, wherein the Chinese herbal medicine preparation is orally taken according to 100mg/5 kg of body weight every time, 3 times a day and is continuously used for 4-7 days.

In addition, the invention provides application of the Chinese herbal medicine preparation for treating influenza in preparing a medicine for treating influenza.

Preferably, the Chinese herbal medicine preparation for treating influenza is an ointment.

(1) The herba Houttuyniae is a herb recorded in Chinese pharmacopoeia, and is derived from dried aerial parts of houttuynia cordata of Saururaceae. Yu xing Cao is pungent in flavor and cold in nature and enters lung meridian. It has effects of clearing away heat and toxic materials, relieving swelling, treating sore, inducing diuresis, removing dampness, clearing away heat, relieving dysentery, invigorating stomach, and resolving food stagnation, and can be used for treating lung abscess, pyocutaneous disease, hemorrhoid hematochezia, and heat accumulation of spleen and stomach due to excess heat, heat toxin, dampness, and disease heat. Modern pharmacological experiments show that the product has antibacterial, antiviral, immunity improving, and diuretic effects.

(2) Herba Taraxaci, herba Violae, herba Veronicae Didyma, and HUALANG, and can be used as perennial herb of Compositae. The dandelion plant contains various health nutritional components such as taraxol, taraxacin, choline, organic acid, inulin, etc. Sweet in nature and taste, slightly bitter and cold. It enters liver and stomach meridians. Has diuretic, laxative, jaundice treating, and gallbladder promoting effects. It can be used for treating heat toxin, carbuncle, pyocutaneous disease, internal carbuncle, conjunctival congestion, swelling and pain, damp-heat, jaundice, stranguria with urine, odynuria, common cold, fever, acute tonsillitis, and acute bronchitis.

(3) Scutellaria baicalensis, also known as root of Camellia japonica or root of Tujin tea, is a perennial herb of Scutellaria in Labiatae. The root of scutellaria is bitter in taste and cold in nature, has the efficacies of resisting bacteria and diminishing inflammation, and has the efficacies of clearing heat and drying dampness, purging fire and removing toxicity, stopping bleeding, preventing miscarriage and the like. It can be used for treating epidemic febrile disease, upper respiratory infection, cough due to lung heat, yellow gallbladder due to damp-heat, pneumonia, dysentery, hemoptysis, conjunctival congestion, threatened abortion, hypertension, carbuncle, furuncle, and sore. The clinical antibacterial property of scutellaria is better than that of coptis, and the scutellaria does not produce drug resistance.

(4) Rhubarb is the name of a plurality of perennial plants in rhubarb of Polygonaceae, and is also the name of traditional Chinese medicinal materials. Has effects in removing food stagnation, clearing away heat and dampness, purging pathogenic fire, cooling blood, removing blood stasis, and removing toxic materials. It can be used for treating heat accumulation, chest distress, dysentery, conjunctival congestion, and sore throat.

(5) The bupleuri radix is dried root of Bupleurum scorzonerifolium or Bupleurum scorzonerifolium of Umbelliferae. Bitter in property and slightly cold in nature. It enters liver and gallbladder meridians. Has the effects of harmonizing exterior and interior, soothing liver and invigorating yang. Can be used for treating common cold, fever, alternating chills and fever, distending pain in chest and hypochondrium, menoxenia, prolapse of son and sense organs, and proctoptosis.

(6) Fructus forsythiae is shrub deciduous in Forsythia of Oleaceae of twisted order of dicotyledons. Has effects of clearing away heat and toxic materials, resolving carbuncle, resolving hard mass, and dispelling pathogenic wind and heat. Opening the bowels and dissipating nodulation, which are the hallucinogen for sore house; has light weight, floats upwards, clears heart fire and can cool and disperse upper energizer. Therefore, it is the essential herb for the onset of sores, abscess and wind-warm syndrome. It can clear heart fire, relieve sore and toxicity, and dissipate qi and blood coagulation, and also has the actions of resolving carbuncle and dissipating nodulation.

(7) Tataria edulis, also known as Shisanchi and Shihu ear, is a perennial herb with thick rhizome. China, Guangdong, Guangxi, Guizhou, Hunan, southeast, Sichuan, east and northwest Hubei. Grows on stone or under the forest beside the ditch in the lime mountain forest and has the elevation of 100-1500 meters. Folk herbs are used for treating cough and lung-heat.

(8) Bamboo rat oil, named as traditional Chinese medicine, is a plant of the family of the order rodentia, the bamboo rat of the family of the order rodentia, which inhabits under the miscanthus or in the bamboo forest. It is eaten by bamboo shoot and Chinese silvergrass. Fat is called Zhu rat oil and can be used as external medicine. Can be used for treating scald, burn, and itching and pain caused by mosquito, insect or insect bite, and has effects of removing toxic substance, relieving itching and relieving pain after application. The drugs are administered with fat. The functions are mainly used for detoxifying, expelling pus, promoting granulation and relieving pain.

(9) The cortex Fraxini is called WUJIYE, CHOUHUANGPI leaf, caulis et folium Gaultheriae Yunnanensis, and FANGZIXIANGCAO, and is branches and leaves of Rutaceae plant Pseudoclausena lansium. Has the functions of dispelling wind and cold, promoting qi circulation to relieve pain, and eliminating dampness and swelling. Can be used for treating common cold, fever, malaria, gastralgia, edema, and rheumatic arthritis. It is used externally to treat fracture, sprain, contusion and eczema.

(10) Herba Commelinae, herba Ardisiae Japonicae, herba magpie, herba Cymbopogonis Citrari, herba Lobeliae chinensis, semen Sedi viridis, herba Clerodendri Trichotomi, herba Siphonostegiae, herba Phyllostachydis Zeylanicae, herba Lophatheri, and is dried aerial part of herba Commelinae of Commelinaceae. Light smell, bland taste. Sweet, bland and cold in nature and taste. It enters lung, stomach and small intestine meridians. Has the effects of clearing away heat and toxic materials, inducing diuresis and relieving swelling. Can be used for treating wind-heat type common cold, hyperpyrexia, sore throat, edema, oliguria, stranguria with heat, pain, carbuncle, and furuncle.

(11) Herba Artemisiae Scopariae is tender dry grass with part of short root, and is curled into a dough. Lignified, hard, brownish surface and yellowish white interior. The most seedling stems and petioles are clustered, and the most seedling stems and petioles are as thin as lines. The length is about 3-10 cm. The leaves are deep-cracked in the shape of two-time pinnate, splintered linear and tippy at the tip. The surfaces of the stems and leaves are covered with grey-white hairy antler. Is soft like velvet. Light faint scent and slightly bitter taste. Clear damp-heat and alleviate jaundice. Has antipyretic, antibacterial, and antiviral effects, and can be used for treating jaundice, oliguria, and eczema pruritus; infectious icteric hepatitis.

(12) Honey is honey produced by bees from flowers of flowering plants in a honeycomb. Can improve blood components, promote functions of heart and brain and blood vessel, supplement physical strength, eliminate fatigue, and enhance resistance to diseases

The invention has the beneficial effects that:

1. the raw materials of the invention are Chinese herbal medicines with homology of medicine and food, and the invention has the advantages of safety, no toxic and side effect, no pollution, no drug residue and no tolerance

Has high medicinal property, high bioavailability and remarkable curative effect. Overcomes the defects that the existing influenza drugs in the market are easy to generate drug resistance and have large side effect.

2. The pill is prepared from concentrated solution and extract preparation of the medicine, has the characteristics of easy absorption of decoction and quick response, has the characteristics of lasting pill effect, medicinal material conservation and convenient carrying and taking, and is a good medicine for treating influenza.

3. Clinical application proves that the Chinese medicinal preparation has remarkable curative effect on influenza, has no side effect, and effectively ensures the physical health of people.

Drawings

FIGS. 1-5 show the results of the safety test of example 4; the dosage of the drug combination in fig. 1 is 0mg/kg, the dosage of the drug combination in fig. 2 is 2500 mg/kg, the dosage of the drug combination in fig. 3 is 5000 mg/kg, the dosage of the drug combination in fig. 4 is 10000 mg/kg, and the dosage of the drug combination in fig. 5 is 20000 mg/kg.

FIGS. 6 to 7 show the results of the PCR assay of the inhibition test of the Chinese medicinal preparation against influenza virus in example 7;

m in fig. 6: marker 2000, 1: positive control, 2: negative control, 3-5: blank control, 6-8: tamiflu administration group, 9-11: 250 mg/kg preventive administration group, 12-14: 500 mg/kg prophylactic administration group;

m in fig. 7: marker 2000, 1: positive control, 2: negative control, 3-5: 1000 mg/kg preventive administration group, 6-8: virus control group, 9-11: 250 mg/kg treatment administration group, 12-14: 500 mg/kg treatment group, 15-17: 1000 mg/kg treatment group.

FIGS. 8-16 show the results of the body weight changes of mice tested for the inhibition of influenza virus by the Chinese medicinal formulation of example 7; FIG. 8 is the change in body weight of mice in the blank control group, FIG. 9 is the change in body weight of mice in the virus control group, FIG. 10 is the change in body weight of mice in the Taffy control group, FIG. 11 is the change in body weight of mice in the 250 mg/kg test group upon prophylactic administration, FIG. 12 is the change in body weight of mice in the 500 mg/kg test group upon prophylactic administration, FIG. 13 is the change in body weight of mice in the 1000 mg/kg test group upon prophylactic administration, FIG. 14 is the change in body weight of mice in the 250 mg/kg test group upon therapeutic administration, FIG. 15 is the change in body weight of mice in the 500 mg/kg test group upon therapeutic administration, and FIG. 16 is the change in body weight of mice in the 1000 mg/kg test group upon therapeutic administration. (Note: since 3 were killed per group at day 6, the body weight of only 5 mice per group was changed).

Detailed Description

The invention will now be described in further detail with reference to the following examples, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The experimental methods used in the following examples are all conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example 1A Chinese herbal preparation for the treatment of influenza is prepared

A Chinese herbal medicine preparation for treating influenza is prepared by the following steps:

(1) weighing the following components in parts by weight: 10 g of houttuynia cordata extract, 10 g of dandelion extract, 10 g of scutellaria baicalensis extract, 8g of rheum officinale, 10 g of radix bupleuri, 10 g of fructus forsythiae, 10 g of ears of cattle, 10 g of clausena lansium, 8g of dayflower, 8g of oriental wormwood, 5g of honey and 1 g of bamboo rat oil.

(2) Adding radix et rhizoma Rhei, bupleuri radix, fructus forsythiae, herba Arctii, cortex Phellodendri, herba Commelinae and herba Artemisiae Scopariae into drinking water, soaking for 20-30 min, adding water to submerge the materials, decocting the casserole on fire, boiling for 40-50 min, and pouring out the medicinal liquid to obtain first medicinal liquid; adding warm water into the casserole, decocting for the second time, boiling for 30 min, pouring out the medicinal liquid, mixing with the first medicinal liquid, naturally cooling, decocting for two times, and mixing the decoctions.

(3) Adding herba Houttuyniae extract, herba Taraxaci extract and Scutellariae radix extract, mixing, adding decocted Mel, stirring while pouring, kneading with food-grade gloves, and adding small amount of bamboo rat oil and pill (100 mg per pill).

Example 2

(1) Weighing the following components in parts by weight: herba Houttuyniae extract 15g, herba Taraxaci extract 15g, and Scutellariae radix extract

15g of the Chinese medicinal composition, 15g of rheum officinale, 5g of radix bupleuri, 5g of fructus forsythiae, 5g of bovine ears, 5g of clausena lansium, 15g of dayflower, 15g of oriental wormwood, 20g of honey and 5g of bamboo rat oil.

(3) Adding herba Houttuyniae extract, herba Taraxaci extract and Scutellariae radix extract, mixing, adding decocted Mel, stirring while pouring, kneading with sterilized hands, and adding small amount of bamboo rat oil and pill (100 mg per pill).

Example 3

(1) Weighing the following components in parts by weight: 12 g of houttuynia cordata extract, 12 g of dandelion extract, 12 g of scutellaria baicalensis extract, 10 g of rheum officinale, 8g of radix bupleuri, 8g of fructus forsythiae, 8g of ears of cattle, 8g of clausena lansium, 10 g of dayflower, 10 g of oriental wormwood, 10 g of honey and 3 g of bamboo rat oil.

Examples of the applications

Example 4 safety experiments

The Chinese herbal medicine preparation for treating influenza prepared in the embodiment 1 of the invention is used for acute toxicity test of mice, and the using steps and the effects are as follows:

(1) taking 50 mice of white mouse species, wherein the weight of each mouse is about 18-20g, each mouse is half male and female, each mouse is 10, and each mouse is randomly divided into 5 groups, namely a blank control group and 4 administration groups (the dosage of the administration groups is 0, 2500, 5000, 10000 and 20000 mg/kg body weight respectively). Fasting (free drinking) is carried out for 6 hours before and after administration, the administration amount of the test group is calculated according to the amount of each mouse, and the metal stomach irrigator is used for intragastric administration once. The control mice remained free to eat and drink water.

(2) And (6) obtaining the result. No abnormal reaction is found in 5 groups of mice through observation, and no abnormal phenomena such as skin, excrement, respiration, limb movement, behavior modes and the like are found in the mice; mice lost weight after dosing and recovered from day 2, with a tendency to gain weight (see figures 1-5), and no mice died in each group. The result shows that the pill has no toxic or side effect on oral administration of mice.

Example 5 test for antipyretic Effect

The Chinese herbal medicine preparation for treating influenza prepared in the embodiment 1 of the invention is tested for antipyretic effect, and the using steps and the effects are as follows:

(1) wistar rats were selected. Selecting 30 Wistar male rats as observation samples of the group of researches, wherein each rat has the weight of about 240 g; dividing the rats into a blank group, a model group and an administration group according to the basal body temperature of the rats, wherein the blank group, the model group and the administration group are respectively 10; model group and administration group.

(2) And (4) carrying out an experimental process. Rats were divided into 10 groups each of a blank group, a model group and a dosing group based on their basal body temperature. Before the experiment, the fasting is not forbidden for 12 h, the administration group 1h before the model building is used for preventive administration twice (with an interval of 0.5 h), the stomach is perfused and administered according to 500 mg/kg (dosage of grinding powder), and the blank group and the model group are perfused with distilled water with the same volume. After the 2 nd administration, 2 mL of each of the fever models was prepared by injecting 2, 4-dinitrophenol (20 mg/kg) subcutaneously into the back of each of the groups except the blank group, and an equal volume of physiological saline was injected into a normal control group. And (5) measuring the anal temperature of the rat at 0.5, 1, 2 and 3 hours after the model is made.

(3) And (6) obtaining the result. The results were recorded based on the measured body temperature and are shown in table 1. The result shows that the body temperature of the rat is in an increasing trend when the administration group is used for 0.5 h after the model building, the body temperature is gradually reduced after the administration group is moved up and down, the body temperature of the rat can be greatly reduced together with the administration group, and the antipyretic effect is good.

TABLE 1 antipyretic test results on rats

Example 6 anti-inflammatory Effect test

The anti-inflammatory effect of the Chinese herbal medicine preparation for treating influenza prepared in the embodiment 1 of the invention is tested, and the using steps and the effects are as follows:

(1) the mice were grouped. 24 Kunming mice, half of which are male and female, are randomly divided into 2 groups, one group is a control group and is coated with normal saline, and the other group is a test group and is orally taken with a Chinese herbal medicine preparation.

(2) And (4) testing. The mice in the experimental group are respectively administrated with the Chinese herbal medicine preparation by oral gavage for 5 days according to 500 mg/kg and 1 time per day; the control group was gavaged with an equal amount of physiological saline. At 40 min after the last administration, 0.05 mL of xylene is coated on both sides (0.025 mL on each side) of the left ear of each mouse to cause inflammation, the mouse is killed 60 min after the inflammation, the ear piece is punched at the same position of the left ear pinna and the right ear pinna of the mouse by an 8 mm puncher, the mass is weighed by an electronic analytical balance, and the difference of the mass of the left ear piece and the right ear piece is the swelling degree.

(3) And (6) obtaining the result. The weight difference between the two ears is swelling degree, and the difference is obvious compared with the normal saline group, and the result shows that the Chinese herbal medicine preparation can obviously inhibit acute inflammation of the ear shell caused by dimethylbenzene (see table 2).

TABLE 2 test results on the effects on inflammation in mice

Example 7 Virus inhibition assay

The Chinese herbal medicine preparation for treating influenza prepared in the embodiment 1 of the invention is used for inhibiting influenza virus, and the using steps and the effects are as follows:

(1) H1N1 influenza virus GX6 strain mouseHalf lethal dose LD₅₀Measurement of (2)

The experiment was divided into 7 groups of 8 female BALB/c mice each, at 6-8 weeks, 18-20g body weight, and the stable titer of 26 cytotoxic drug was diluted 10-fold to 10-fold in order from the original toxin^-5BALB/c mice were vaccinated. A control group was also set.

Treating with dry ice before inoculation, and after mice syncope, grouping according to different viruses by nasal drip method, and administering 50 μ L of virus to each mouse. Body weight changes and death were recorded daily after receiving the virus, 14 days after recording and observation, according to Reed&LD calculated by Muench method₅₀。

Results half of mice lethal dose LD of H1N1 influenza virus GX6 strain₅₀Is 10^-3.58。

(2) Test of drug efficacy

And (5) grouping the tests. The BALB/c mouse species of 72 mice were selected, half-and-half, each weighing about 18-20g, and randomly divided into 9 groups. Group 1 is blank control group, group 2 is virus control group; the group 3 is a tamiflu administration group, and the concentration of the administered tamiflu is 50 mg/kg;

groups

4, 5 and 6 are prophylactic administration groups, administered at doses of 250, 500 and 1000 mg/kg, respectively;

groups

7, 8 and 9 are treatment groups administered at doses of 250, 500 and 1000 mg/kg, respectively.

Counteracting toxic substances. On

days

1 and 2, the preventive administration groups of

groups

4, 5 and 6 were orally drenched at prescribed doses, 2 times daily, and the other groups were normally kept. 3, inoculating toxin on day, namely, anesthetizing with dry ice, and after the mouse syncope, connecting 50 mu L of physiological saline in a 1 st group by adopting a nose dropping method; groups 1 to 9 were inoculated with H1N1 influenza virus GX6 at a concentration of 5 LD per inoculation₅₀Mice were inoculated with 50. mu.L each.

And (4) administration.

Group

1 and 2 were not administered; the preventive administration groups of

groups

4, 5 and 6 were each drenched orally at the prescribed dose 2 times daily followed by 6 days of administration. On the 2 nd day after toxin counteracting, the 3 rd group is orally fed with duffy, the administration concentration is 50 mg/kg, 1 time per day, and the feeding is continuously carried out for 7 days;

groups

7, 8 and 9 were fed with herbal preparations, each administered orally at the prescribed dose 2 times daily, followed by 7 days.

And (6) recording. Observing whether symptoms (including messy quilt, mental depression, crouching of the back and the back, etc.) appear, weighing, recording death, weight, body temperature, diet and mental status every day, and continuously observing for 14 days. And at 6 days after the challenge, 3 mice per group were randomly selected for killing, and lung tissues were taken for virus detection.

And (5) detecting the tissue virus. Weighing the obtained lung tissue, adding normal saline according to the proportion of 20% emulsion, completely grinding by a grinder, centrifuging for 3min at the speed of 2000 r/min, taking supernatant, and performing virus detection by using PCR.

(3) Results

The PCR detection result. Influenza virus nucleic acid was detected positively in lung tissue of mice in the virus control group, the duffy administration group, the 250 mg/kg preventive administration group, the 250 mg/kg therapeutic administration group, and the 500 mg/kg therapeutic administration group, and the remaining groups were negative (see fig. 6 and 7). The results show that the 500 mg/kg, 1000 mg/kg preventive administration group and 1000 mg/kg treatment administration group have obvious inhibition effect on the proliferation of influenza virus in mice, and the preventive administration effect is more obvious than the therapeutic administration effect.

② weight change. All mice died on day 8 of the virus control group; mice in the blank control group, the 500 mg/kg prevention administration group, the 1000 mg/kg treatment administration group and the Tamiflu administration group begin to rise again in body weight on day 4 and return to normal on days 7-14; 1 mouse still died in the 250 mg/kg preventive administration group and the 500 mg/kg treatment administration group, and the rest 4 mice recovered to normal on days 7-14; the 250 mg/kg treatment group still had 2 deaths, 2 of the remaining 3 mice recovered to normal on days 7-14, while the other 1 mice had no body weight recovery at the end of the test (see FIGS. 8-16). The result shows that the Chinese herbal medicine preparation for treating influenza has good prevention and treatment effects on influenza.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.

Claims

1. The Chinese herbal medicine preparation for treating influenza is characterized by being prepared from the following raw materials in parts by weight: 10-15 parts of houttuynia cordata extract, 10-15 parts of scutellaria baicalensis extract, 10-15 parts of dandelion extract, 8-15 parts of rheum officinale, 5-10 parts of radix bupleuri, 5-10 parts of fructus forsythiae, 5-10 parts of oxear, 5-10 parts of clausena lansium, 8-15 parts of dayflower, 8-15 parts of oriental wormwood, 5-20 parts of honey and 1-5 parts of bamboo rat oil.

2. A method of preparing a herbal formulation for the treatment of influenza as claimed in claim 1 comprising the steps of:

(1) weighing the components according to the weight parts of the raw materials;

(3) adding herba Houttuyniae extract, herba Taraxaci extract and Scutellariae radix extract, mixing, adding the decocted Mel, stirring while stirring, mixing, kneading, rolling, and adding bamboo rat oil and pill.

3. Use of a herbal formulation according to claim 1 for the treatment of influenza in the manufacture of a medicament for the treatment of influenza.

4. The herbal formulation for treating influenza of claim 1, wherein said herbal formulation for treating influenza is a paste.