CN104644759A - Health tea for preventing and/or treating influenza and preparation method thereof - Google Patents
Health tea for preventing and/or treating influenza and preparation method thereof Download PDFInfo
- Publication number
- CN104644759A CN104644759A CN201410613353.9A CN201410613353A CN104644759A CN 104644759 A CN104644759 A CN 104644759A CN 201410613353 A CN201410613353 A CN 201410613353A CN 104644759 A CN104644759 A CN 104644759A
- Authority
- CN
- China
- Prior art keywords
- radix ginseng
- flos lonicerae
- health tea
- preparation
- influenza
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a health tea for preventing and/or treating influenza and a preparation method thereof. The health tea is prepared from honeysuckle flowers and weeping forsythia leaves, wherein the honeysuckle flowers and the weeping forsythia leaves are soaked by a ginseng extract solution. The cold nature of the honeysuckle flowers and the weeping forsythia leaves is balanced or counteracted by the hot nature of ginseng, thus the health tea is rich in aroma, is warm in nature and is not cold and hot, can be drunk to treat influenza and enhance immunity in the influenza season, is suitable for people to drink in daily life and has the function of preventing and/or treating influenza. The health tea has a significant effect, is convenient to drink, is good in taste and has no side and toxic effects.
Description
Technical field
The present invention relates to a kind of prevention or/and treat grippal health product and preparation method thereof, be particularly a kind ofly derived from the prevention of plant or treat grippal health tea and preparation method thereof, belong to medicinal tea, beverage and functional food field.
Background technology
Flos Lonicerae, formal name used at school (Japan-ese Honeysuckle), has another name called Radix Ophiopogonis, is perennial half evergreen woody plants of Caprifoliaceae." Flos Lonicerae " one comes from Compendium of Material Medica, owing to opening as white at the beginning of Flos Lonicerae, after transfer yellow to, hence obtain one's name Flos Lonicerae.Medical material Flos Lonicerae is Caprifoliaceae woodbine Radix Ophiopogonis and congener dry flower or is with the flower just opened.Flos Lonicerae is described as the good medicine of heat-clearing and toxic substances removing from ancient times.Its property sweet cold fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as fever of the body, dermexanthesis, sends out the diseases such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, equal Be very effective.Flos Lonicerae contains a large amount of material useful to human body, comprising chlorogenic acid, isochlorogenic acid, sweet-scented osmanthus oxalic acid, luteolin-glucoside, xylostein, saponin, volatile oil, inositol, tannin, iridoid glycoside driffractive ring meliatin, sweroside etc., belong to rare Chinese medicine, area, Yi Mengshan Mountain is the main place of production.
Flos Lonicerae is just extensive and famous with its medical value since ancient times.Shennong's Herbal carries: " Flos Lonicerae sweet in the mouth cold in nature, has effect of heat-clearing and toxic substances removing, removing heat from bloodization silt, cure mainly affection due to external wind and heat, pestilence from the beginning of, skin infection furunculosis, red and swollen heat pain, passing stool with blood and pus " etc.Flos Lonicerae is discussed in detail and has the effect of " for a long time clothes make light of one's life by commiting suicide, life lengthening " in Compendium of Material Medica.The 1980s, health ministry has successively carried out chemical analysis to Flos Lonicerae, result shows: Flos Lonicerae contains the necessary trace element of multiple human body and chemical composition, simultaneously containing the multiple organized enzyme material favourable to human body, there is defying age, anti-canceration, the good effects of being healthy and strong of making light of one's life by commiting suicide.
Folium Forsythiae belongs to the dry blade of Oleaceae plants Fructus Forsythiae, gathers according to " Sichuan Province's Chinese crude drug standard ", and according to " Sichuan Province's prepared slices of Chinese crude drugs concocted specification " processing.
According to the record of Compendium of Material Medica, " Chinese medicine voluminous dictionary ", Shennong's Herbal, Fructus Forsythiae stem is produced in SiChuan, leaf can control cardiopulmonary accumulated heat, clearing away summer-heat, promoting the production of body fluid to quench thirst, sore-throat relieving wet one's whistle, the long-term taking face of making us is pleased, improving eyesight clears away heart-fire, it is always resistance to make light of one's life by commiting suicide, and makes us calling forth one's youthful vigor.
Radix Ginseng is Araliaceae herbaceos perennial, and the ancient pharmacy ancient books and records Shennong's Herbal about Chinese medicine of China is classified as Radix Ginseng top grade, says its " determine soul, spasmolytic is throbbed with fear, improving eyesight, happy Fructus Alpiniae Oxyphyllae, and clothes have the effect of making light of one's life by commiting suicide and prolonging life for a long time for main tonifying five ZANG-organs, peace spirit ".Show through modern medicine study and assay, containing a kind of chemical substance of being ginsenoside in Radix Ginseng, it has obvious effect to the central nervous system, heart tonifying, resisting fatigue, Auto-regulator metabolism etc. of mediator, so to treatment nervous system. and the various diseases of cardiovascular system, hormonal system and reproductive system has good therapeutical effect.
Radix Ginseng extract soaks Flos Lonicerae, Folium Forsythiae, and cold, dry property can be made to offset, temperature and cold, not hot-tempered, three kinds of Chinese medicine jointly play anti-ly to cure cold, constitutional effect.
Summary of the invention
Main purpose of the present invention is for above-mentioned prevention or/and treat prior art and the product Problems existing of grippal health product and Chinese medicine preparation, provide a kind of for preventing or/and health tea treating influenza and preparation method thereof.The health tea set preventing and treating influenza of the present invention is improved human body immunity, effect of prevention, treatment influenza is remarkable, the dry property of the cold and Radix Ginseng of Flos Lonicerae, Folium Forsythiae mutually balances or offsets, aromatic thick, the temperature of tea and cold, not dry, is applicable to the daily tea-drinking of people.And prevention of the present invention or/and the preparation method of health tea for the treatment of influenza is easy, stable processing technique, cheap and easy to carry and use
For realizing object of the present invention, one aspect of the present invention provides a kind of prevention or/and treat grippal health tea, comprises following raw material: Flos Lonicerae, Fructus Forsythiae, Radix Ginseng.
Wherein, the weight of described raw material is: Flos Lonicerae: Folium Forsythiae: Radix Ginseng is 100:100:1-10, is preferably 100:100:2-5.
Particularly, Folium Forsythiae selected by described Fructus Forsythiae; Described Radix Ginseng select in Radix Ginseng stem, Folium Ginseng or Radix Ginseng one or more.
Of the present inventionly prevent and treat Flos Lonicerae, Folium Forsythiae, Radix Ginseng in the health tea of influenza and combinationally use, make effect of each medicine produce synergistic function, improve prevention, treat grippal efficiency.
Flos Lonicerae another name Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, two precious flowers are the flower that caprifoliaceae plant Radix Ophiopogonis, Flos Lonicerae, Flos Lonicerae (hair calyx Radix Ophiopogonis) or the dry flower of hair style Radix Ophiopogonis or band are just opened.Cold in nature, sweet in the mouth; Return lung, the heart, stomach warp.Have heat-clearing and toxic substances removing, effect of wind-heat dissipating, be mainly used in carbuncle furuncle, sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease is generated heat.
Flos Lonicerae be used for affection due to external wind and heat or epidemic febrile disease from the beginning of; Sore and toxic, laryngopharynx swelling and pain; The dysentery that pyretic toxicity causes is had blood in stool (accompanying mucus and blood in feces).Both can relieving the exterior syndrome clearly thoroughly, can noxious heat in the blood system be separated again, particularly control the key medicine of positive skin infection.
The chemical composition of Flos Lonicerae mainly contains luteolin, chlorogenic acid, isochlorogenic acid, Ginnol, cupreol, stigmasterol, cupreol-D-Glucose glycoside, stigmasterol-D-Glucose glycoside etc., inositol about 1% and Saponin, tannin etc.
Modern pharmacological research shows that Flos Lonicerae has 1. resisting pathogenic microbes effect: experiment in vitro shows that Flos Lonicerae various pathogens such as staphylococcus aureus, Hemolytic streptococcus, escherichia coli, dysentery bacterium, vibrio cholera, Bacillus typhi, Salmonella paratyphi etc. all have certain inhibitory action, to streptococcus pneumoniae, meningococcus, bacillus pyocyaneus, tubercule bacillus is also effective.Water logging agent is stronger than decoct effect, and leaf decoct is stronger than colored decoct effect.2. antiinflammatory and refrigeration function: lumbar injection Flos Lonicerae extractive solution 0.25g/kg, can suppress Rat Carrageenan colloidality swelling of the feet.3. the function that strengthens the defense effect: Flos Lonicerae decoct is diluted to the concentration of 1:1280, still can promote leukocytic phagocytic function.Mouse peritoneal injection honeysuckle flower injection, also has the effect obviously promoting inflammatory cell phagocytic function.4. central excitation effect: after the kinds of experiments such as galvanic shock, rotating cage method proves oral chlorogenic acid, the animal central nervous systems such as rat, mice can be caused excited, and its action intensity is 1/6 of caffeine.5. effect for reducing blood fat: rat oral gavage Flos Lonicerae 2.5g/kg can reduce enteral cholesterol absorption, reduces cholesterol level in blood plasma.
Fructus Forsythiae sticks up also known as Fructus Forsythiae, Hemerocallis citrina Baroni bar, yellow chain barriness, Huang Qidan, green grass or young crops, falls to sticking up, and is the dry fruit of Oleaceae plants Fructus Forsythiae.Cold nature, bitter in the mouth, return lung, the heart, small intestine meridian.There is heat-clearing and toxic substances removing, the function of dispersing swelling and dissipating binds, be mainly used in treating carbuncle, scrofula, acute mastitis, erysipelas, anemopyretic cold, epidemic febrile disease from the beginning of, warmly join the army, high hot excessive thirst, coma sends out speckle, pyretic stranguria urine retention.Fructus Forsythiae bitter in the mouth cool in nature, light clear floats, and can control all heat of the part of the body cavity above the diaphragm housing the heart and lungs, especially can removing toxicity for eliminating carbuncles and eliminating stagnation.
Fructus Forsythiae is containing Neolignans: forsythin, phillygenin, dextrorotation pinoresinol, dextrorotation (+)-Pinoresinol glycoside; Flavone compound: globulariacitrin; Vinylbenzene analog derivative: Fructus Forsythiae fat glycoside A, C, D, E, Fructus Forsythiae kind glycoside, salidroside; Cyclohexyl alcohol derivatives: (wood comes) wooden glycoside, Fructus Forsythiae Hexalin, different Fructus Forsythiae Hexalin, Fructus Forsythiae cyclohexanol thing, Fructus Forsythiae hexamethylene alcohol ketone, Fructus Forsythiae Hexalin glycoside A, B, C; Triterpenoid compound: white firelight or sunlight fat acid, oleanolic acid, ursolic acid, β-Amyrin acetas, Isobauerenyl Acetate, 20 (S)-Da Ma-24-alkene-3 β, 20-glycol-3-acetas etc.Blue or green Fructus Forsythiae contains Saponin 4.89%, alkaloid 0.2%.
Fructus Forsythiae has 1. antibacterial, antivirus action: Fructus Forsythiae concentrated decotion has antibacterial action in vitro, can suppress Bacillus typhi, Salmonella paratyphi, escherichia coli, dysentery bacterium, diphtheria corynebacterium and vibrio cholera, staphylococcus, streptococcus etc.; Volatile Oil of Forsythia Suspensa Seeds Emulsion all has to capital section 68-1 strain and celestial platform strain virus the antivirus action shown outside Embryo Gallus domesticus.2. Fructus Forsythiae has impact to cardiovascular system, has heart tonifying and boosting; 3. the capillary permeability caused by Fructus Forsythiae injection Dichlorodiphenyl Acetate has obvious inhibitory action.4. Fructus Forsythiae has inhibitory action to guinea pig in vitro small intestinal; 5. the effect of elastin laminin enzyme activity is suppressed; 6. Antiradiation injury.Fructus Forsythiae methanolic extract 1g/kg lumbar injection, in roentgen radiation x administration in first 5 minutes, has protective action to mouse skin infringement.
Radix Ginseng is Araliaceae herbaceos perennial, and the ancient pharmacy ancient books and records Shennong's Herbal about Chinese medicine of China is classified as Radix Ginseng top grade, says its " determine soul, spasmolytic is throbbed with fear, improving eyesight, happy Fructus Alpiniae Oxyphyllae, and clothes have the effect of making light of one's life by commiting suicide and prolonging life for a long time for main tonifying five ZANG-organs, peace spirit ".Radix Ginseng is flat, sweet in the mouth, micro-hardship; Return spleen, lung, heart channel.Have strongly invigorating primordial QI, multiple arteries and veins takes off admittedly, and invigorating the spleen to benefit the lung, promotes the production of body fluid, the function of calming the nerves.Be mainly used in weak body and prostration, cold extremities faint pulse, insufficiency of the spleen lack of appetite, the deficiency of the lung is breathed with cough, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, prolonged illness weakness with emaciation, palpitation with fear insomnia, sexual impotence cold womb; Heart failure, cardiogenic shock.
Radix Ginseng contains ginsenoside and a small amount of volatile oil, and in oil, Main Ingredients and Appearance is panacene; Soaping agents is isolated according to reports: ginsenoside A, B, C, D, E and F etc. from root.Produce Panaxatriol ginsenoside unit after Ginsenoside B and C are hydrolyzed, obtain 20-after ginsenoside D, E and F hydrolysis and show ginseng diol saponin unit.Beta-elemene is isolated again from the low boiling part of ether extract.High-boiling fration isolates Panaxynol.In addition still containing monosaccharide: glucose, fructose, sugarcane candy, three kinds of trisaccharides: Glucose-Fructose-fructose, three polydextrose, glucose-glucose-fructose, panax acid (for palmitic acid, stearic acid and linoleic mixture), many clocks vitamin (B
1, B
2, Yan acid, nicotinic acid amide, pantothenic acid), several amino acids, choline, enzyme (maltase, invertase, esterase), spermine and cholamine.The aerial parts of Radix Ginseng is containing flavone compound, and people claims the yellow glycoside of Radix Ginseng, trilobatin, kaempferol, ginsenoside, cupreol and saccharide.
The main composition of Radix Ginseng is ginsenoside (Ginsenoside), and modern pharmacological research proves that it has effects such as improving immunity, strengthening by means of tonics, blood circulation promoting and blood stasis dispelling, resisting fatigue.
The present invention provides a kind of prevention or/and treat the preparation method of grippal health tea on the other hand, comprises the steps:
1) crude drug is prepared according to following weight:
Flos Lonicerae 100, Fructus Forsythiae 100, Radix Ginseng 1-10
2) carry out heating extraction after people being participated in Extraction solvent mixing, collect extracting solution;
3) extracting solution is concentrated, make Radix Ginseng extract extractum;
4) Radix Ginseng extract extractum is mixed homogeneously with water or alcoholic solution, make Radix Ginseng extract solution;
5) Flos Lonicerae, Fructus Forsythiae are mixed homogeneously with Radix Ginseng extract solution, obtained Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture, then carries out drying and processing, to obtain final product.
Wherein, step 1) described in the weight of crude drug be: Flos Lonicerae 100, Fructus Forsythiae 100, Radix Ginseng 2-5.
Particularly, Folium Forsythiae selected by described Fructus Forsythiae; Described Radix Ginseng select in Radix Ginseng stem, Folium Ginseng or Radix Ginseng one or more.
Wherein, step 2) described in Extraction solvent select water or alcoholic solution.
Particularly, described alcoholic solution selection mass percent concentration is ethanol-water solution or the methanol-water solution of 1-80%, and being preferably mass percent concentration is 10-60% ethanol-water solution or methanol-water solution.
Wherein, in described heating extraction process, Extracting temperature is 85-100 DEG C, is preferably 90-100 DEG C; Extraction time is 2-3 time; Each extraction time is 30-90min.
Particularly, the weight that in each heating extraction process, people participates in Extraction solvent is 8-12:1.
Particularly, also comprise after people is participated in Extraction solvent mixing, after soaking 30-60min, carrying out described heating extraction.
Wherein, described heating extraction comprises the step that following order is carried out:
A) join in Extraction solvent by Radix Ginseng, the Extraction solvent added is 8-12 with the ratio of the weight of Radix Ginseng:
1, carry out first time heating extraction after soaking 30-60min, heating-up temperature is 85-100 DEG C, and the heating extraction time is 30-90min;
B) first time extracting solution is filtered, collect filtrate;
C) in filtering residue, add Extraction solvent 1-2 time, carry out heating extraction, wherein, the weight of the Extraction solvent at every turn added is 8-12:1 with the ratio of the weight of Radix Ginseng, and heating-up temperature is 85-100 DEG C, and the heating extraction time is 30-90min;
D) filter, the filtrate after filtering is merged, obtains Radix Ginseng extractive solution.
Wherein, step 3) described in the relative density of Radix Ginseng extract extractum be 1.05-1.25, being concentrated into relative density by Radix Ginseng extractive solution is 1.05-1.25; In described concentration process, thickening temperature is 80-90 DEG C.
Wherein, step 4) described in alcoholic solution select mass percent concentration to be the alcoholic solution-aqueous solution of 30-50%; Described water selective temperature is the hot water of 50-70 DEG C, is preferably 60 DEG C of hot water.After Radix Ginseng extract extractum mixes with water or alcoholic solution, dissolve, make Radix Ginseng extract solution.
Particularly, the weight of described Radix Ginseng extract extractum and water or alcoholic solution is 1-60:100, is preferably 10-50:100.
Wherein, step 4) described in the concentration of Radix Ginseng extract solution be 1-60% (w/v), be preferably 10-50% (w/v).
Wherein, step 5) in also comprise and be immersed in described Radix Ginseng extract solution and carry out described drying and processing by mixing homogeneously Flos Lonicerae, Fructus Forsythiae with described Radix Ginseng extract solution again after 30-60min.
Particularly, the temperature of described immersion is 30-50 DEG C.
Wherein, step 5) described in the temperature of drying and processing be 60-90 DEG C.
Particularly, also comprise the mixture after by oven dry pulverize and cross 10 mesh sieves.
The active component preventing and treating influenza health tea of the present invention can add various customary adjuvant required when preparing different dosage form, as disintegrating agent, lubricant, adhesive etc. are prepared into the conventional peroral dosage form of any one, as pill, powder, tablet, granule, capsule, oral liquid etc. with the method for Chinese medicinal of routine.
Health tea of the present invention has dispelling wind-heat, removing summer-heat blood poison, and expelling pathogenic factors from the exterior is gone out, mass dissipating and swelling eliminating, removes the functions such as pathogen, for prevention, treatment influenza.
When prepared by the present invention prevents and treats the use of influenza health tea, oral 2-3 time of every day, each 2-5 gram (crude drug), can use as daily beverage, enhancing human body immunity power continuously, prevention, treatment influenza.。
The health tea set preventing and treating influenza of the present invention has the following advantages and benefit:
1, health tea prescription compatibility science of preventing and treating influenza of the present invention, determined curative effect, safety is high, quality controllable.
2, of the present inventionly prevent and treat Flos Lonicerae in the health tea of influenza, the dry property of cold and Radix Ginseng of Folium Forsythiae mutually balances or offset, aromatic thick, the temperature of tea and cold, not dry, be applicable to the daily tea-drinking of people.
3, the present invention's preparation prevents and treats Flos Lonicerae in the process of the health tea of influenza, Folium Forsythiae is soaked in after in Radix Ginseng extract solution, direct and Radix Ginseng extract is dried in the lump, without extraction, without any the loss of composition, farthest remain the effective ingredient of medicine, give full play to the effect of its influenza.
4, the invention provides a kind of new Flos Lonicerae, the application form of Folium Forsythiae.
5, the health tea preventing and treating influenza that prepared by the present invention, adopt that crude drug is rationally composite forms, set about from Radix Ginseng adjusting immunity of human body, pass through Flos Lonicerae, the Chinese medicine combined effect of the heat-clearing and toxic substances removing that Folium Forsythiae these two is classical, reach the object of prevention, treatment influenza, therefore, product of the present invention has the multi-efficiency for the treatment of both the principal and secondary aspects of a disease, prevention and therapy influenza.
6, preparation method of preventing and treating the health tea of influenza of the present invention is simple, and process conditions are gentle, low cost of manufacture.
Detailed description of the invention
Further describe the present invention below in conjunction with specific embodiment, advantage and disadvantage of the present invention will be more clear along with description.But these embodiments are only exemplary, do not form any restriction to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments and replacement all fall within the scope of protection of the present invention.
Embodiment 1
1, (× 100g) is got the raw materials ready according to following weight proportion
Flos Lonicerae 100 Folium Forsythiae 100 Stem and leaf of Radix Ginseng 5
2, Radix Ginseng extract extractum is prepared
2-1) join in Multifunctional Chinese medicine extraction pot by raw medicinal material Stem and leaf of Radix Ginseng 500g and tap water, under room temperature (15-30 DEG C), soak 30min, wherein tap water is 12:1 with the ratio of the weight of Stem and leaf of Radix Ginseng;
2-2) open multi-function extractor power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to extract 60min, then filter, obtain first time extracting solution;
2-3) in filtering residue, point add tap water 2 times, heating extraction 2 times, the temperature of each heating extraction is 100 DEG C, and the weight of the tap water added is 12:1 with the ratio of Stem and leaf of Radix Ginseng weight, and extraction time is 1 hour, filters and obtains 2 extracting solution;
Extracting solution 2-4) heated 3 times merges, and heat after obtaining total extracting solution, carry out evaporation and concentration process, obtain the extractum (162g) that relative density is 1.1, wherein, thickening temperature is 90 DEG C;
3, Radix Ginseng extract solution is prepared
To step 2) to add temperature in the Radix Ginseng extract extractum prepared be 60 DEG C of hot water, stir, be uniformly dissolved, make Radix Ginseng extract solution (540ml), wherein the concentration of Radix Ginseng extract solution is 30% (w/v);
4, Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture is prepared
Flos Lonicerae, Folium Forsythiae are mixed with Radix Ginseng extract solution, and soaks with Radix Ginseng extract solution, stir, soak 60min at 30 DEG C after, obtain Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture; In immersion process, Radix Ginseng extract solution all absorbs by Flos Lonicerae, Folium Forsythiae.
5, drying and processing
Flos Lonicerae-Fructus Forsythiae-Radix Ginseng the mixture of acquisition is placed in baking oven, carries out normal pressure drying and processing, and wherein bake out temperature is 90 DEG C, dry to moisture content be 12%;
Mixture after drying is pulverized and packed after crossing 10 mesh sieves, makes the health tea that 100,000 bags are prevented and treated influenza.
Embodiment 2
1, (× 100g) is got the raw materials ready according to following weight proportion
Flos Lonicerae 100 Folium Forsythiae 100 Stem and leaf of Radix Ginseng 3
2, Radix Ginseng extract extractum is prepared
2-1) join in Multifunctional Chinese medicine extraction pot by raw medicinal material Radix Ginseng 300g and tap water, soak 45min, wherein tap water is 10:1 with the ratio of the weight of Stem and leaf of Radix Ginseng;
2-2) open multi-function extractor power supply to heat, be heated to 90 DEG C, keep constant temperature at this temperature to extract 90min, then filter, obtain first time extracting solution;
2-3) in filtering residue, point add tap water 2 times, heating extraction 2 times, the temperature of each heating extraction is 90 DEG C, and the weight of the tap water added is 10:1 with the ratio of Radix Ginseng weight, and extraction time is 90min, filters and obtains 2 extracting solution;
Extracting solution 2-4) heated 3 times merges, and heat after obtaining total extracting solution, carry out evaporation and concentration process, obtain the extractum (87g) that relative density is 1.2, wherein, thickening temperature is 80 DEG C;
3, Radix Ginseng extract solution is prepared
To step 2) add in the Radix Ginseng extract extractum prepared 30% ethanol-water solution, stir, be uniformly dissolved, make Radix Ginseng extract solution (174ml), wherein the concentration of Radix Ginseng extract solution is 50% (w/v);
4, Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture is prepared
Flos Lonicerae, Folium Forsythiae are mixed with Radix Ginseng extract solution, and soaks with Radix Ginseng extract solution, stir, soak 40min at 50 DEG C after, obtain Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture; In immersion process, Radix Ginseng extract solution all absorbs by Flos Lonicerae, Folium Forsythiae.
5, drying and processing
Flos Lonicerae-Fructus Forsythiae-Radix Ginseng the mixture of acquisition is placed in baking oven, carries out normal pressure drying and processing, and wherein bake out temperature is 80 DEG C, dry to moisture content be 15%;
Mixture after drying is pulverized and packed after crossing 10 mesh sieves, makes the health tea that 100,000 bags are prevented and treated influenza.
Embodiment 3
1, (× 100g) is got the raw materials ready according to following weight proportion
Flos Lonicerae 100 Folium Forsythiae 100 Stem and leaf of Radix Ginseng 2
2, Radix Ginseng extract extractum is prepared
2-1) join in Multifunctional Chinese medicine extraction pot by raw medicinal material Stem and leaf of Radix Ginseng 200g and tap water, soak 60min, wherein tap water is 8:1 with the ratio of the weight of Stem and leaf of Radix Ginseng;
2-2) open multi-function extractor power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to extract 30min, then filter, obtain first time extracting solution;
2-3) in filtering residue, point add tap water 2 times, heating extraction 2 times, the temperature of each heating extraction is 100 DEG C, and the weight of the tap water added is 8:1 with the ratio of Radix Ginseng weight, and extraction time is 30min, filters and obtains 2 extracting solution;
Extracting solution 2-4) heated 3 times merges, and heat after obtaining total extracting solution, carry out evaporation and concentration process, obtain the extractum (82g) that relative density is 1.05, wherein, thickening temperature is 85 DEG C;
3, Radix Ginseng extract solution is prepared
To step 2) add the ethanol-water solution that mass percent concentration is 30% in the Radix Ginseng extract extractum prepared, stir, be uniformly dissolved, make Radix Ginseng extract solution (820ml), wherein the concentration of Radix Ginseng extract solution is 10% (w/v);
4, Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture is prepared
Flos Lonicerae, Folium Forsythiae are mixed with Radix Ginseng extract solution, and is soaked in Radix Ginseng extract solution, stir, soak 30min at 50 DEG C after, obtain Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture; In immersion process, Radix Ginseng extract solution all absorbs by Flos Lonicerae, Folium Forsythiae.
5, drying and processing
Flos Lonicerae-Fructus Forsythiae-Radix Ginseng the mixture of acquisition is placed in baking oven, carries out normal pressure drying and processing, and wherein bake out temperature is 85 DEG C, dry to moisture content be 9%;
Mixture after drying is pulverized and packed after crossing 10 mesh sieves, makes the health tea that 100,000 bags are prevented and treated influenza.
Embodiment 4
1, (× 100g) is got the raw materials ready according to following weight proportion
Flos Lonicerae 100 Folium Forsythiae 100 Stem and leaf of Radix Ginseng 4
2, Radix Ginseng extract extractum is prepared
2-1) join in Multifunctional Chinese medicine extraction pot by raw medicinal material Radix Ginseng 400g and tap water, soak 60min, wherein tap water is 12:1 with the ratio of the weight of Stem and leaf of Radix Ginseng;
2-2) open multi-function extractor power supply to heat, be heated to 85 DEG C, keep constant temperature at this temperature to extract 90min, then filter, obtain first time extracting solution;
2-3) in filtering residue, add tap water, heating extraction 1 time, heating extraction temperature is 85 DEG C, and the weight of the tap water added is 12:1 with the ratio of Radix Ginseng weight, and extraction time is 90min, filters and obtains the 2nd extracting solution;
Extracting solution 2-4) heated 2 times merges, and heat after obtaining total extracting solution, carry out evaporation and concentration process, obtain the extractum (118g) that relative density is 1.1, wherein, thickening temperature is 80 DEG C;
3, Radix Ginseng extract solution is prepared
To step 2) add the ethanol-water solution that mass percent concentration is 50% in the Radix Ginseng extract extractum prepared, stir, be uniformly dissolved, make Radix Ginseng extract solution (295ml), wherein the concentration of Radix Ginseng extract solution is 40% (w/v);
4, Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture is prepared
By Flos Lonicerae, Folium Forsythiae, mix with Radix Ginseng extract solution, and be soaked in Radix Ginseng extract solution, stir, soak 50min at 40 DEG C after, obtain Flos Lonicerae-Fructus Forsythiae-Radix Ginseng mixture;
5, drying and processing
Flos Lonicerae-Fructus Forsythiae-Radix Ginseng the mixture of acquisition is placed in baking oven, carries out normal pressure drying and processing, and wherein bake out temperature is 70 DEG C, dry to moisture content be 14%;
Mixture after drying is pulverized and packed after crossing 10 mesh sieves, makes the health tea that 100,000 bags are prevented and treated influenza.
Test example 1 prevents and treats the research of influenza health tea resisiting influenza virus effect
1. experiment material
1.1 medicine
Prevent and treat influenza health tea: Dalian Fu Sheng natural drug development corporation, Ltd. produces, lot number: 20130121.
Ribavirin injection, colourless transparent liquid, effluent Nan Run expands limited company and produces, batch number: 1206261, the accurate word of traditional Chinese medicines: H19993553,100mg/ml, as this test positive control medicine.
Above-mentioned medicine all uses dissolved in purified water, and filter, degerming subpackage, 4 DEG C for subsequent use, for this tests medicine to be measured.
1.2 cell strain
Vero (African green monkey kidney cell cell) preserves cell strain by preclinical medicine institute of Jilin University.
1.3 Strain
1) influenza virus is purchased from Inst. of Viruses, China Preventive Medicine Science Academy.
2) parainfluenza virus is purchased from Inst. of Viruses, China Preventive Medicine Science Academy.
3) respiratory syncytial virus (RSV) is purchased from Inst. of Viruses, China Preventive Medicine Science Academy.
1.4 instruments and reagent
Culture medium: DMEM, HyClone
Hyclone: HyClone
Trypsin: Gibco
MTT：Sigma
DMSO: Tianjin Bei Lian fine chemicals development corporation, Ltd.
Biohazard Safety Equipment: BHC-1300 II A/B3, AIRTECH
CO
2incubator: MCO-18AIC, SANYO
Inverted microscope: CKX41, OLYMPUS
Electronic analytical balance: AR1140/C, DHAUS
2. experimental technique
2.1 cells prepare
Vero passage cultivates 1-2d, make it in blocks, boundary line is clear, third dimension and diopter strong time, with trypsinization, treat that needle point sample aperture appears in cell face, exhaust Digestive system, peek milliliter culture fluid dispels cell, and counting, is diluted to about 5 × 10 with culture fluid (DMEM containing 10% hyclone)
7after individual/L, be inoculated in 96 well culture plates, treat that cell grows up to monolayer.
2.2 drug toxicitys measure
Cell toxicity test: medicine maintenance medium (DMEM containing 2% hyclone) is diluted, for cytotoxic assay by concentration shown in table 1.
Table 1 drug dilution reference table (unit: g/L)
The medicine of variable concentrations good for above-mentioned dilution is dripped on Vero cell monolayer, every hole 0.2ml, the multiple hole of each concentration 6, separately establishes 6 hole normal controls (not adding the Normal group of medicine) and 6 hole blanks (culture fluid), put 37 DEG C, 5%CO
2cultivate in incubator, put inverted microscope every day and observe CPE and record.After 72h, every hole adds MTT solution 20 μ L (5mgmL
-1), continue to hatch 4h, inhale and abandon each hole culture fluid, every hole adds 100 μ L DMSO, and vibration 5min, 492nm measure OD value, calculate cell survival rate.In SPSS18.0 statistical software, cell survival rate is carried out Probit regression analysis, calculate medicine to the maximal non-toxic concentration (TC of Vero cell
0) and half toxic concentration (TC
50).
The impact of 2.3 drug on viral cytopathic effects
Get the culture plate covering with cell monolayer, draw culture fluid, with 100TCID
50corresponding virus attack amount inoculating cell, 37 DEG C, 5%CO
2incubator absorption 2h, adds each medicinal liquid of certain concentration (about maximal non-toxic concentration), and the multiple hole of every concentration 6 is cultivated, 200 μ L/ holes.If ribavirin injection is positive drug control group, establishes Normal group (not adding virus not dosing) and virus control group (add virus but do not add the matched group of medicine) simultaneously, observe the impact that drug on viral causes CPE.After 72h, use MTT colorimetry, under 492nm wavelength, measure OD value, calculate Antiviral Effect effective percentage (ER%).By the significant difference between the more each Antiviral Effect effective percentage of ANOVA method in SPSS18.0 statistical software.
ER%=(drug treating group mean OD value-virus control group mean OD value)/(cell controls group mean OD value-virus control group mean OD value) × 100%
3. experimental result
3.1 drug toxicity measurement results
Each medicine is to the maximal non-toxic concentration (TC of Vero cell
0), half toxic concentration (TC
50) and Antiviral Effect experiment concentration in table 2.Prevent and treat influenza health tea for measuring half toxic concentration (TC
50), its safety is good.
Table 2 drug cytotoxicity experimental result (unit: g/L)
* prevent and treat influenza health tea and do not measure half toxic concentration (TC
50)
3.2 drug on viral cytopathogenic effect protective effect results
Prevent and treat influenza health tea infected by influenza, parainfluenza virus, respiratory syncytial virus all have good inhibitory action, its resisiting influenza virus, respiratory syncytial virus are all better than ribavirin, and it is suitable with ribavirin to the effective percentage of parainfluenza virus.Experimental result is in table 3.
Table 3 Antiviral Effect effective percentage (ER%) statistical table
*P<0.05,**P<0.01
Test example 2. Antiviral assay in vivo
1 experiment material
1.1 medicines and reagent
Prevent and treat influenza health tea: Dalian Fu Sheng natural drug development corporation, Ltd. produces, lot number: 20130121;
Positive control drug: ribavirin injection, effluent Nan Run expands limited company and produces, batch number: 1206261, the accurate word of traditional Chinese medicines: H19993553.
1.2 instrument
1.3 animal
Kunming mouse male and female half and half, body weight 15-20g, purchased from Dalian Medical Univ's Experimental Animal Center, laboratory animal credit number: SCXK (the Liao Dynasty) 2004-0017.
2. experimental technique
2.1 animal groupings
Get the kunming mice 240 in surrounding age, carry out 2 tests.Get mice 120, be divided into 12 groups at random, often organize 10, the first six group is respectively: Normal group, virus control group, ribavirin group, prevent and treat the high, medium and low dosage group of influenza health tea, for preventing and treating the determination test of influenza health tea influenza virus infected Lung Exponent and lung index; And after six groups being respectively Normal group, virus control group, ribavirin group, preventing and treating the high, medium and low dosage group of influenza health tea, for preventing and treating the determination test of influenza health tea to parainfluenza virus infection mouse lung exponential sum lung index; Separately get mice 120, be divided into 12 groups at random, often organize 10, after above-mentioned packet mode grouping, for preventing and treating the determination test of influenza health tea influenza virus infected and parainfluenza virus infection mouse lung suspension viral hemoagglutination titre.
2.2 infection methods
To except Normal group, other group mices carry out infection process, concrete operations are: in the beaker of 200 ~ 300mL size, put into an absorbent cotton, then pour appropriate ether (making absorbent cotton become wet) into, the beaker back-off that absorbent cotton is housed is come, mice is put into and anaesthetizes, see that mice is on wires, time obviously in unable sample, lain on the back by mice, collunarium infects 15LD
50influenza virus or parainfluenza virus 0.03ml/ nostril, Normal group normal saline replaces viral suspension.
2.3 medications and dosage
Prevent and treat the high, medium and low dosage group of influenza health tea and ribavirin matched group, conventional gastric infusion is started the previous day respectively at infecting, prevent and treat the high, medium and low dosage of influenza health tea be respectively 0.26,0.13,0.065g/kg, ribavirin positive drug dosage is 58.5mg/kg, once a day, successive administration 5d, Normal group and virus control group gavage the normal saline of same volume.
2.4 observation index
1. Lung Exponent measures
After mice medication the 5th day, first fasting prohibited water 8 hours, plucks eyeball sacrificed by exsanguination animal after weighing, open thoracic cavity and extract full lung, with brine twice, blot surperficial moisture content with filter paper, electronic balance claims lung weight, calculates Lung Exponent and lung index by following formulae discovery:
Lung Exponent=(mouse lung weight/Mouse Weight) × 100%.
Lung index=(the average Lung Exponent of the average Lung Exponent-experimental group of infection model group)/average Lung Exponent × 100% of infection model group.
2. lung suspension viral hemoagglutination titer determination
Get the treatment each group of mouse lung of latter 5th day respectively, the underlying homogenizer of low temperature grinds to form homogenate, normal saline dilution is the lung tissue suspension of 10%, centrifuging and taking supernatant, doubling dilution, drips on titer plate by 0.2ml/ hole, every hole adds 0.2ml1% chicken erythrocyte suspension, mixing, puts room temperature 30min, observed and recorded Hemagglutination titer.With red cell agglutination (++) Shi Wei Final point, represents its titre with suspension extension rate.
3. experimental result and analysis
3.1 Lung Exponent measure
After influenza virus and parainfluenza virus infection mice; average Lung Exponent result display: compare with the model control group infecting virus; Normal group, each concentration and preventing influenza health tea group, ribavirin group Lung Exponent obviously reduce (P<0.05); prevent and treat influenza health tea concentration and have certain protective role within the scope of 0.065 ~ 0.26g/kg/d; Lung Exponent all obviously reduces; high dose group curative effect is best, in, low dose group and ribavirin therapeutic equivalence.Experimental result is in table 4,5.
The impact of influenza health tea influenza virus infected Lung Exponent and lung index prevented and treated by table 4
* represent that comparing # represents to compare with ribavirin group with virus control group
The impact of influenza health tea on parainfluenza virus infection mouse lung exponential sum lung index prevented and treated by table 5
* represent and compare with virus control group
3.2 lung suspension viral hemoagglutination titer determinations
After influenza virus and parainfluenza virus infection mice, matched group lung tissue viral hemoagglutination titre (InX) infecting virus is respectively 33.11 and 32.45, variable concentrations is prevented and treated after influenza health tea treats 5 days, lung tissue viral hemoagglutination titre declines all to some extent, compare with virus control group, difference has significance, (P<0.05), middle and high dosage group better efficacy, low dose group and ribavirin therapeutic equivalence.Experimental result is respectively in table 6, table 7.
The impact of influenza health tea influenza virus infected lung suspension Hemagglutination titer prevented and treated by table 6
* represent that comparing # represents to compare with ribavirin group with virus control group
The impact of influenza health tea on parainfluenza virus infection mouse lung suspension Hemagglutination titer prevented and treated by table 7
* represent that comparing # represents to compare with ribavirin group with virus control group
Test example 3 prevents and treats the research that influenza health tea improves immunity function
1. test material
1) medicine and reagent
Prevent and treat influenza health tea: Dalian Fu Sheng natural drug development corporation, Ltd. produces, lot number: 20130121;
Positive control drug: Polysaccharide-peptide capsule (manufacturer: Shanghai Xinkang Pharmaceutical Factory, lot number: 20110306, specification: 0.5g/ grain etc.)
Hydrolyzed protein: (manufacturer: Guangxi Kangzhe Guangming Pharmaceutical Co., Ltd., lot number: 20110208, specification: 10ml*6 props up/box)
Chicken red blood cell: (manufacturer: south, Yuhuan county chemical reagent work, lot number: 20101208, specification: 100ml/ bottle)
MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide bromine salt): (Suo Laibao bio tech ltd, Shanghai of manufacturer, lot number 20101105, specification: 250mg)
2) tumor source S180 sarcoma model, Lewis lung cancer C57BL/6 mouse model to go down to posterity maintenance by pharmacological room of Shanghai Institute of Pharmaceutical Industry.
3) laboratory animal
C57BL/6 mice, Kunming mouse (male), body weight 18-20 gram, purchased from Dalian Medical Univ's Experimental Animal Center, laboratory animal credit number: SCXK (the Liao Dynasty) 2004-0017.
2. experimental technique
The Peritoneal Macrophage Phagocytosis test of 2.1 pairs of normal Kunming mouses
Get Male Kunming strain mice 60, be divided into 5 groups at random, be respectively: prevent and treat the high, medium and low dosage group of influenza health tea, positive controls, negative control group, often organize 10 for first four groups, negative control group is 20;
Prevent and treat influenza health tea high, medium and low dosage group, polysaccharide-peptide group, the equal gastric infusion of negative control group, continuous 10 days, dosage was as shown in table 8, and negative control group gives equal-volume pure water;
After last administration, each group mouse peritoneal only injects 0.5% hydrolyzed protein 1.5ml/, and within 24 hours, pneumoretroperitoneum injects every milliliter 1 × 10
6chicken erythrocyte suspension 0.2ml/ only; With normal saline eluting, collection mouse peritoneal liquid after 40 minutes; Centrifugal, get cell precipitation liquid and make smear, fix with methanol, Giemsa staining mounting, count in 100 macrophages with oily mirror and engulf chicken erythrocytic macrophage number and engulf the erythrocytic sum of chicken, by following formulae discovery percentage phagocytosis and phagocytic index.
Percentage phagocytosis %=(engulfing erythrocytic macrophage number/100 of a chicken macrophage in 100 macrophages) × 100%;
Erythrocytic sum/100 of a chicken macrophage is engulfed in the macrophage of phagocytic index=100.
The NK cell activity assays of 2.2 pairs of lotus Lewis lung cancer C57BL/6 mices
Get C57BL/6 mice 60, the Lewis lung cancer suspension 0.05ml/ of the aseptic preparation of toe subcutaneous vaccination only (about 1 × 10
6individual tumor cell);
Next day is divided into 5 groups at random, is respectively: prevent and treat the high, medium and low dosage group of influenza health tea, positive controls, negative control group, and often organize 10 for first four groups, negative control group is 20;
Prevent and treat influenza health tea high, medium and low dosage group, polysaccharide-peptide group, the equal gastric infusion of negative control group, continuous 10 days, dosage was as shown in table 9, and negative control group gives equal-volume pure water;
After last administration, next day aseptically gets mouse spleen, makes single splenocyte suspension with 100 eye mesh screens, and hypotonic removing erythrocyte, proceeds to cell suspension in culture bottle, 37 DEG C of 5%CO
2cMC model removed attached cell after 1 hour, living cell counting to adjust cell concentration be 3 × 10
6individual cell/ml action effect cell.Target cell gets L929 cultured cell in vitro, cellar culture 24 hours, with culture fluid adjustment cell concentration for 1.5 × 10
5individual cell/ml, makes effect target cell ratio be 20: 1.Get 96 well culture plates and add effector lymphocyte and target cell respectively, another laying effect cell and target cell contrast, in 37 DEG C of 5%CO
2cMC model, after 4 hours, adds MTT dyeing liquor, then adds Digestive system after cultivating 2 hours and survey each hole OD value, by formulae discovery NK cytotoxic activity next day.
NK cytotoxic activity %={ [target cell matched group OD average-(experimental group OD average-effector lymphocyte OD average)]/target cell matched group OD average } × 100%
3. experimental result
3.1 prevent and treat the phagocytic function of influenza health tea to normal Kunming mouse peritoneal macrophage
Compared with negative control group, prevent and treat after influenza health tea and polysaccharide-peptide medication through variable concentrations, its phagocytic percentage and phagocytic index all have remarkable lifting, difference has significance, (P<0.01), illustrate and prevent and treat the effect that influenza health tea and polysaccharide-peptide all have the phagocytic function improving normal mouse peritoneal macrophage, middle and high dosage group better efficacy, low dose group and polysaccharide-peptide group therapeutic equivalence.Experimental result is respectively in table 8.
The impact of influenza health tea on normal Kunming mouse Peritoneal Macrophage Phagocytosis prevented and treated by table 8
* * * P<0.01 compared with negative control group
The NK cell activity assays of 3.2 pairs of lotus Lewis lung cancer C57BL/6 mices
Compared with negative control group, prevent and treat after influenza health tea and polysaccharide-peptide medication through variable concentrations, its NK cytoactive all has remarkable lifting, difference has significance, (P<0.01), illustrate and prevent and treat the effect that influenza health tea and polysaccharide-peptide all have the NK cytoactive improving lotus Lewis lung cancer C57BL/6 mice, prevent and treat influenza health tea group curative effect and be better than polysaccharide-peptide group.Experimental result is respectively in table 9.
The impact of influenza health tea on lotus Lewis lung cancer mice NK activity prevented and treated by table 9
1 compared with negative control group * * * P<0.01;
The OD value of 2# refers to (effector lymphocyte+target cell)-effector lymphocyte.
Claims (10)
1. prevention is or/and treat a grippal health tea, it is characterized in that comprising following crude drug: Flos Lonicerae, Folium Forsythiae, Radix Ginseng.
2. health tea as claimed in claim 1, is characterized in that the weight of described crude drug is 100:100:1-10.
3. prevention is or/and treat a preparation method for grippal health tea, it is characterized in that comprising the steps:
1) crude drug is prepared according to following weight:
Flos Lonicerae 100, Folium Forsythiae 100, Radix Ginseng 1-10
2) carry out heating extraction after people being participated in Extraction solvent mixing, collect extracting solution;
3) extracting solution is concentrated, make Radix Ginseng extract extractum;
4) Radix Ginseng extract extractum is mixed homogeneously with water or alcoholic solution, make Radix Ginseng extract solution;
5) join in Radix Ginseng extract solution by Flos Lonicerae, Folium Forsythiae, stirring, mix homogeneously, obtained Flos Lonicerae-Folium Forsythiae-Radix Ginseng mixture, then carries out drying and processing, to obtain final product.
4. preparation method as claimed in claim 3, is characterized in that step 1) described in the weight of crude drug be: Flos Lonicerae 100, Folium Forsythiae 100, Radix Ginseng 2-5.
5. the preparation method as described in claim 3 or 4, is characterized in that step 1) described in Radix Ginseng select in Radix Ginseng stem, Folium Ginseng or Radix Ginseng one or more.
6. the preparation method as described in claim 3 or 4, is characterized in that step 2) described in Extraction solvent select water or alcoholic solution.
7. the preparation method as described in claim 3 or 4, is characterized in that step 3) described in the relative density of Radix Ginseng extract extractum be 1.05-1.25.
8. the preparation method as described in claim 3 or 4, is characterized in that step 4) described in the concentration of Radix Ginseng extract solution be 1-60%.
9. the preparation method as described in claim 3 or 4, is characterized in that step 5) in also comprise Flos Lonicerae, Folium Forsythiae be immersed in 30-60min in described Radix Ginseng extract solution, then carry out described drying and processing.
10. the preparation method as described in claim 3 or 4, is characterized in that step 5) in drying and processing temperature be 60-90 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410613353.9A CN104644759B (en) | 2013-11-22 | 2014-11-04 | A kind of health protection tea for preventing or/and treating influenza and preparation method thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013105899974 | 2013-11-22 | ||
| CN201310589997 | 2013-11-22 | ||
| CN201410613353.9A CN104644759B (en) | 2013-11-22 | 2014-11-04 | A kind of health protection tea for preventing or/and treating influenza and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104644759A true CN104644759A (en) | 2015-05-27 |
| CN104644759B CN104644759B (en) | 2018-01-09 |
Family
ID=53236728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410613353.9A Active CN104644759B (en) | 2013-11-22 | 2014-11-04 | A kind of health protection tea for preventing or/and treating influenza and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104644759B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509198A (en) * | 2015-09-15 | 2017-03-22 | 郭进军 | Formula preparation technology and usage of health-care tea |
| CN106615431A (en) * | 2016-12-08 | 2017-05-10 | 山东新糖源农业科技有限公司 | Sliced helianthus tuberosus tea and making method thereof |
| CN114177220A (en) * | 2020-09-14 | 2022-03-15 | 富力 | Composition of human and forsythia fruit and its antiviral medicine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010083662A (en) * | 2000-02-17 | 2001-09-01 | 손남율 | Antiviral and Anticancer Agents |
| CN101152261A (en) * | 2007-09-04 | 2008-04-02 | 北京理工大学 | Application of phenyl ethyl alcohol glycosides and xylogen glycosides component in forsythia (fruit or leaf) |
-
2014
- 2014-11-04 CN CN201410613353.9A patent/CN104644759B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010083662A (en) * | 2000-02-17 | 2001-09-01 | 손남율 | Antiviral and Anticancer Agents |
| CN101152261A (en) * | 2007-09-04 | 2008-04-02 | 北京理工大学 | Application of phenyl ethyl alcohol glycosides and xylogen glycosides component in forsythia (fruit or leaf) |
Non-Patent Citations (2)
| Title |
|---|
| 孙平华: "中药抗流感病毒的最新进展", 《中医药学刊》 * |
| 潘曌曌等: "金银花、连翘配伍提取物体外抗甲型流感病毒FM1株的实验研究", 《甘肃中医学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509198A (en) * | 2015-09-15 | 2017-03-22 | 郭进军 | Formula preparation technology and usage of health-care tea |
| CN106615431A (en) * | 2016-12-08 | 2017-05-10 | 山东新糖源农业科技有限公司 | Sliced helianthus tuberosus tea and making method thereof |
| CN114177220A (en) * | 2020-09-14 | 2022-03-15 | 富力 | Composition of human and forsythia fruit and its antiviral medicine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104644759B (en) | 2018-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102139030B (en) | Oral Chinese herbal preparation for treating gingivitis and preparation method thereof | |
| CN102716223B (en) | Chinese medicinal composition for treating upper respiratory tract infection and preparation method thereof | |
| CN104689226A (en) | Traditional Chinese medicine composition for treating children with rheumatoid arthritis and preparation method thereof | |
| CN102166278A (en) | Chinese medicinal composition for treating pediatric rheumatoid arthritis and preparation method thereof | |
| CN104645107A (en) | Traditional Chinese medicine composition, and preparation method and application thereof | |
| CN104644759A (en) | Health tea for preventing and/or treating influenza and preparation method thereof | |
| CN101293065A (en) | Biological proprietary Chinese medicine for preventing and treating diabetes | |
| CN108813501A (en) | With the relieving cough and reducing sputum health honey paste relievingd asthma and adjust function of human body of clearing heat and moistening lung | |
| CN104489172A (en) | Acanthopanax health tea for improving immunity and preparation method thereof | |
| CN105878922A (en) | Traditional Chinese medicinal injection for treating ischemic stroke with syndromes of stagnation and obstruction of heat-toxin and blood stasis | |
| CN104888158A (en) | Folium apocyni veneti oral liquid for enhancing immunity and preparation method thereof | |
| CN104983968A (en) | Pyretolysis traditional Chinese medicine composition and preparation method | |
| CN104474404A (en) | Compound suppositorycontrollinginfectionof immunodeficiency patient | |
| CN104721508B (en) | A kind of pharmaceutical composition and preparation method for treating the infection of the upper respiratory tract | |
| CN106177651A (en) | A kind of infantile malnutrition medicine | |
| CN104623068A (en) | Medicine for treating infantile fever and convulsion and preparation method of medicine | |
| CN110237157B (en) | Chinese herbal medicine preparation for treating influenza and preparation method thereof | |
| CN105617319A (en) | Preparation method of traditional Chinese medicine composition for treating acute cholecystitis | |
| CN103230580B (en) | Traditional Chinese medicine composition for preventing and treating infectious serositis of duck | |
| CN106389533A (en) | Medicine for treating Parkinson's disease | |
| CN106511829A (en) | Yao medicine tub-bath preparation capable of reducing blood pressure, blood sugar and blood fat, and preparation method thereof | |
| CN105853843A (en) | Pharmaceutical composition for clinical pediatric wound care and preparation method of pharmaceutical composition for clinical pediatric wound care | |
| CN105106906A (en) | Traditional Chinese medicine preparation for treating excessive internal heat | |
| CN105582379A (en) | Traditional Chinese medicine composition for treating liver-stomach disharmony and preparation method of traditional Chinese medicine composition | |
| CN105213627A (en) | A kind of Chinese medicine being used for the treatment of sun exterior deficiency |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |