KR20010083662A - Antiviral and Anticancer Agents - Google Patents

Abstract

PURPOSE: Provided are antiviral and antitumor agents which have an inhibiting effect on retrovirus and destroy malignant tumor(cancer) without causing any side effects. And their preparation is also provided. CONSTITUTION: The antiviral and antitumor agents comprise unsaturated fatty acid extract of sesame and alkaline extract of ginseng. The unsaturated fatty acids are extracted from sesame by compressing without heating to produce an unsaturated fatty acids extract. An extract of ginseng is obtained by dipping the ginseng into hot water and then filtering the water through a sanitary cotton to produce an alkaline extract. The amount of water used for such extraction should be about 5 to 1000 times as much as the weight of ginseng. The unsaturated fatty acid extract of sesame and the alkaline extract of ginseng are mixed in a mixing ratio of about 1 to 1-5. The mixed extract is useful for the prevention of HIV, influenza virus, herpes virus, simplex virus, rotavirus and hepatitis virus infections, and the treatment of malignant tumor.

Description

Antiviral and Anticancer Agents {.}

The present invention relates to an antiviral agent and an anticancer agent composed of an alkaline synthetic extract of unsaturated fatty acid of ginseng and ginseng as an active ingredient. Numerous antiviral and anticancer drugs have been known in the past, but antiviral drugs are mainly effective only on bacteria and are effective only on viruses, even if they are effective against viruses. Moreover, antibiotics usually have side effects, which are sometimes very harsh. And can be serious.

Many diseases are caused by viruses such as influenza, anorexia, fatigue, vomiting, neuropharmaceuticals, pancreatic cancer, adult T-cells, diabetes, and AIDS, but unfortunately, drug treatments that show clear efficacy against these conventional viral diseases are There are few drugs that are effective enough, especially against retroviruses that cause antidiabetic or AIDS without any side effects. There was also no known antiviral agent that could be taken in daily diets and had a preventive effect without any side effects. Therefore, studies on the effective treatment of AIDS and AIDS-related complexes that do not have side effects are particularly difficult, and some medications that have limited effects through long-term chemotherapy may have serious side effects. Since anticancer drugs known as anticancer drugs have not only destroyed cancer cells but also damaged normal cells, these anticancer drugs have had severe side effects. In addition, since these anticancer drugs were difficult to use oral administration, there have been many problems with these anticancer drugs. Therefore, there was a need to invent an agent having anti-HIV and anti-cancer agents without side effects and having viral adsorption on cells. It is therefore an object of the present invention to provide an anticancer agent that is effective against retroviruses and destroys malignant tumors (cancer) without side effects by solving the above problems. The present inventors have diligently researched to solve the above problem, and the unsaturated fatty acids of sesame and alkali extracts of ginseng have strong antiviral and anticancer effects, and the unsaturated fatty acids and alkali extracts are extremely safe for animals or eyes and have no side effects. The present invention was developed.

The active ingredient of the present invention is derived from unsaturated fatty acid extracts by pressing the sesame seed and pressing it without heating after drying 15% of moisture. More preferably, ginseng is extracted with hot water, extracted from hot water, and then synthesized with an alkaline solution and unsaturated fatty acid in a ratio of about 1: 1 to 5. Extraction is carried out by pressing without heating and then extracted with unsaturated fatty acid solution. In the extraction process, the amount of water should be 5-1000 times the weight of ginseng and extracted for 5 minutes-10 hours at 70-100 ℃.

Specific examples of unsaturated fatty acids include proteins, amino acids, methionine, substrates, calcium and alkaline solutions. Specific examples of panacene and falcarinol include 28 kinds of saponin components, ginsenosidRx (x = o, a, b1, b2, c, d, e. , f, g1, g3, h1, h2), and the like. Known methods and extraction methods such as hot water extraction can also be used. Separation of the extraction solution can be carried out by known methods such as filtration recantation, centrifugation and the like. Ethanol, isetone, etc. can be used as an extraction solvent on water.

The active ingredient of the extract may be mixed with a suitable carrier such as excipients, binders, or ignition materials, and for example, capsules, ointments, syrups, suppositories, injections, orally or parenterally, or mixed as it is, In the form of a single dose, the concentrate may be used or mixed with other foods.

The dosage depends on the type and severity of the disease. However, in the liquid form, 1 ml to 1 mg to 500 ml should be administered.

Unsaturated fatty acid extracts of sesame and alkali extracts of ginseng were synthesized in a ratio of 1: 1 to 5, and mice and mice were tested. No mortality was observed and abnormal behavior of animals was not observed. No value appears in the experiment.

The present invention is an antiviral agent, an anticancer agent, an antibacterial agent and a bactericide, and an insecticide. According to the present invention, infection of various viruses such as influenza virus herpes, sinplex virus (simple herpes virus), rotavirus, and hepatitis virus is prevented. In addition, it inhibits replication and prevents the growth or coronation of microorganisms such as bacteria and fungi, and also obtains extracts that enhance and treat malignant tumors without causing any side effects.

Example

Manufacturing Example 1

The sesame seeds are washed with water and dried 15% of moisture, then compressed without heating, and the ginseng is washed with water, followed by the drying process of sunlight.

3 g of these dried ginseng are extracted for 3 hours in 100 mu of hot water at 85 ° C., and then filtered through a sanitary cotton (thickness of 0.5 cm) to obtain an extract containing various acids and sugars. Unsaturated fatty acid extracts of sesame and alkali extracts of ginseng are obtained at a ratio of about 1: 1 to 5.

Manufacturing Example 2

As an unsaturated fatty acid and an alkaline solution, an extract synthesized 1: 1 is obtained in the same manner as in Preparation 1.

Manufacturing Example 3

In the same manner as in Preparation 1, an extract obtained by synthesizing the alkaline solution in the ratio of about 1: 1 to 5 was obtained by using one gauze bucket instead of the sanitary cotton for filtration.

Experimental Example 1

The composite obtained in Preparation Example 1 is applied to cultured MDCK cells or MA104 cells. Immediately after application, MDCK- was infected with influenza virus, MA104 was sensitized with hertersinplex virus, and spontaneous incubation with the compound resulted in flaking formation. It is completely (100%) inhibited and is shown to be inhibited by more than 90% by 1ug / m1 of the extract in case of herpes virus flaming.

Experimental Example 2

MT-4 cells (2.5 × 10 ⁴ cells / well) were infected with HIV-1 and the infected cells were spread over a microchiterplate containing 96 wells containing various concentrations of the extract obtained in Preparation Example 1, followed by CO Incubate at 37 ° C. in ₂ incubators for 5 days. The number of viable cells is measured by MTT method. Anti-HIV activity is expressed in concentrations that show 50% protection against HIV-induced cell destruction (EC50: 50% effective concentration). EC 50 is less than 21 ug / m1.

Experimental Example 3

Infect MT-4 cells (2.5 × 10 ⁴ cells / well) with HIV and spread the infected cells immediately after infection with a microtiter plate with 96 wells containing various concentrations of the extract obtained in Preparation Example 2, and CO ₂ Incubate at 37 ° C. for 5 days in the incubator. The number of viable cells is measured by MTT method. Anti-HIV activity is expressed as a concentration that represents 50% protection against HIV-induced cell destruction by test substances. (EC 50: 50% effective concentration) The EC 50 is less than 21 ug / m1.

Experimental Example 4

WVOC (1 × 10 ⁶ cells / m1) extracted from gastric cancer was injected subcutaneously into 20 rats. From the next day, as an experimental group, 10 of these mice were injected daily with 100 mg / m1 of the extract obtained in Preparation 1.

Mice not administered the extract were used as controls. Cell growth is calculated for three weeks after administration of the extract. The number of cells in the control group is significantly increased while the increase of tumor cells in the experimental group is significantly suppressed.

Experimental Example 5

NVGC-4 (1 × 10 ⁶ cells / m 1) isolated from gastric cancer was injected subcutaneously into 20 rats. From the next day, as an experimental group, 10 of these rats are injected daily with the extract of 500 ug / mu obtained in Preparation Example 3. Mice not administered the extract are used as a control group. The growth of the cells after administration of the extract is calculated for 1 month. After one month, the cells in the control group increased markedly and there was some company area, and some animals died, but the number of cells in the experimental group was significantly suppressed, so there was no dead animal.

Experimental Example 6

NVGC-4 (1 × 10 ⁶ cells / mu) extracted from gastric cancer was loaded into a 96 well cyclator plate containing various concentrations of 75-subfraction obtained in Preparation Example 3, followed by CO ₂ incubator at 37 ° C. Incubate for 5 days at. The number of viable cells is measured by MTT method. The anticancer activity of unsaturated fatty acids and acidic polysaccharides is expressed as a concentration (IC 50: 50% effective concentration) that inhibits the growth of cancer cells by 50%. The 75-subfraction IC50DMS 200ug / m1, but the purified unsaturated fatty acids and acidic polysaccharides (25-75% sedimentation) do not inhibit tumor cell growth.

Claims (6)

Antiviral agent consisting of alkaline synthetic extract of unsaturated fatty acid of sesame and ginseng as active ingredient The method of claim 1 Antiviral agent characterized in that the virus is a human immunodeficiency virus (HIV) The method of claim 1 Unsaturated fatty acid solution contains protein, amino acid, methionine, substrate, calcium component and alkaline solution, panacene, falcarinol contains 28 kinds of saponin components, ginsenosidRx (x = o, a, b1, b2, c, d, e, f, g1 , g3, h1, h2, characterized in that the antiviral As an active ingredient Anticancer agent consisting of unsaturated fatty acid of sesame and alkali extract of ginseng Method of producing antiviral agent by squeezing sesame without heating sesame and filtering alkaline solution to hot water extraction of unsaturated fatty acid and ginseng The method of claim 4 Unsaturated fatty acid solution contains protein, amino acid, methionine, substrate, calcium, alkaline solution, panacene, falcarinol, 28 kinds of saponin components, ginsenosidRx (x = o, a, b1, b2, c, d, e, f, g1, g3, h1, h2 is a method for producing an anticancer agent characterized in that