KR20010083663A - Antiviral and Anticancer Agents - Google Patents

Abstract

PURPOSE: Provided are antiviral and antitumor agents which have an inhibiting effect on retrovirus and destroy malignant tumor(cancer) without causing any side effects. And their preparation is also provided. CONSTITUTION: The antiviral and antitumor agents comprise unsaturated fatty acids extract of sesame and alkaline extract of Ganoderma lucidum. The unsaturated fatty acids are extracted from sesame by compressing without heating to produce an unsaturated fatty acids extract. An extract of Ganoderma lucidum is obtained by dipping the Ganoderma lucidum into hot water and then filtering the water through a sanitary cotton to produce an alkaline extract. The amount of water used for such extraction should be about 5 to 1000 times as much as the weight of Ganoderma lucidum. The unsaturated fatty acid extract of sesame and the alkaline extract of Ganoderma lucidum are mixed in a mixing ratio of about 1 to 1-5. The mixed extract is useful for the prevention of HIV, influenza virus, herpes virus, simplex virus, rotavirus and hepatitis virus infections, and the treatment of malignant tumor.

Description

Antiviral and Anticancer Agents {.}

The present invention relates to an antiviral agent and an anticancer agent comprising an unsaturated fatty acid of sesame and an alkaline synthetic extract of Ganoderma lucidum as an active ingredient. Numerous antiviral and anticancer drugs have been known in the past, but antiviral drugs are mainly effective only on bacteria and are effective only on viruses, even if they are effective against viruses. Moreover, antibiotics usually have side effects, which are sometimes very harsh. And can be serious.

Many diseases are caused by viruses such as influenza, insomnia, obesity, anticancer drugs, bronchitis, hypertension, adult T-cells, diabetes, and AIDS, but unfortunately, few drugs show clear efficacy against these conventional viral diseases. There was no medicament that was effective enough against the retrovirus that caused antidiabetic or AIDS, especially without any side effects. There was also no known antiviral agent that could be taken in daily diets and had a preventive effect without any side effects. Therefore, studies on the effective treatment of AIDS and AIDS-related complexes that do not have side effects are particularly difficult, and some medications that have limited effects through long-term chemotherapy may have serious side effects. Since anticancer drugs known as anticancer drugs have not only destroyed cancer cells but also damaged normal cells, these anticancer drugs have had severe side effects. In addition, since these anticancer drugs were difficult to use oral administration, there have been many problems with these anticancer drugs. Therefore, there was a need to invent an agent having anti-HIV and anti-cancer agents without side effects and having viral adsorption on cells. It is therefore an object of the present invention to provide an anticancer agent that is effective against retroviruses and destroys malignant tumors (cancer) without side effects by solving the above problems. The present inventors have diligently researched to solve the above problem, and the unsaturated fatty acids of sesame and alkali extracts of Ganoderma lucidum have strong antiviral and anticancer effects, and the unsaturated fatty acids and alkali extracts are extremely safe and have no side effects on animals or eyes. The present invention was developed.

The active ingredient of the present invention is derived from unsaturated fatty acid extracts by pressing the sesame seed and pressing it without heating after drying 15% of moisture. More preferably, ganoderma lucidum is extracted with warm water, followed by synthesis of an alkaline solution and unsaturated fatty acid at a ratio of about 1: 1 to 5. Extraction is carried out by pressing without heating, followed by extraction with unsaturated fatty acid solution. In the extraction process, the amount of water should be 5-1000 times the weight of Ganoderma lucidum and extracted for 5 minutes-10 hours at 70-100 ° C.

Specific examples of unsaturated fatty acids include polysaccharides, amino acids, methionine, substrates, specific examples of calcium and alkaline solutions include polysaccharides, organic acids, resins, mannitol, coumarin, lactones, and the like. Known methods and extraction methods such as hot water extraction can also be used. Separation of the extraction solution can be carried out by known methods such as filtration recantation, centrifugation and the like. Ethanol, isetone, etc. can be used as an extraction solvent on water.

The active ingredient of the extract may be mixed with a suitable carrier such as excipients, binders, or ignition materials, and for example, capsules, ointments, syrups, suppositories, injections, orally or parenterally, or mixed as it is, In the form of a single dose, the concentrate may be used or mixed with other foods.

The dosage depends on the type and severity of the disease. However, in the liquid form, 1 ml to 1 mg to 500 ml should be administered.

Unsaturated fatty acid extracts of sesame and alkali extracts of Ganoderma lucidum were synthesized in a ratio of 1: 1 to 5, and mice and mice were tested. There is no mortality, no abnormal behavior of animals is observed, blood biochemical test and tissue pathology. No value appears in the experiment.

The present invention is an antiviral agent, an anticancer agent, an antibacterial agent and a bactericide, and an insecticide. According to the present invention, infection of various viruses such as influenza virus herpes, sinplex virus (simple herpes virus), rotavirus, and hepatitis virus is prevented. In addition, it inhibits replication and prevents the growth or coronation of microorganisms such as bacteria and fungi, and also obtains extracts that enhance and treat malignant tumors without causing any side effects.

Example

Manufacturing Example 1

The sesame seed is dried in 15 minutes of moisture and then pressed without heating, followed by the extraction of Ganoderma lucidum, followed by the drying process of sunlight.

3 g of these dried ganoderma lucidum were extracted for 3 hours in 100 mu of hot water at 85 ° C. and then filtered through a sanitary cotton (0.5 cm thick) to obtain an extract containing various acids and sugars. Obtain a synthetic extract of sesame unsaturated fatty acid extract and Ganoderma lucidum alkaline extract at a ratio of about 1: 1 to 5.

Manufacturing Example 2

As an unsaturated fatty acid and an alkaline solution, an extract synthesized 1: 1 is obtained in the same manner as in Preparation 1.

Manufacturing Example 3

In the same manner as in Preparation 1, an extract obtained by synthesizing the alkaline solution in the ratio of about 1: 1 to 5 was obtained by using one gauze bucket instead of the sanitary cotton for filtration.

Experimental Example 1

The composite obtained in Preparation Example 1 is applied to cultured MDCK cells or MA104 cells. Immediately after application, MDCK® is infected with influenza virus, MA104 is sensitized with Hertersinplex virus, and spontaneous incubation with the compound results in flaking. As a result, the influenza virus flag (spot) is completely inhibited by 0.1mg / m1 (100%), and in case of herpes virus flaming is suppressed by more than 90% by 1ug / m1 of the extract.

Experimental Example 2

MT-4 cells (2.5 × 10 ⁴ cells / well) were infected with HIV-1 and the infected cells were spread over a microchiterplate containing 96 wells containing various concentrations of the extract obtained in Preparation Example 1, followed by CO Incubate at 37 ° C. in ₂ incubators for 5 days. The number of viable cells is measured by MTT method. Anti-HIV activity is expressed in concentrations that show 50% protection against HIV-induced cell destruction (EC50: 50% effective concentration). EC 50 is less than 21 ug / m1.

Experimental Example 3

Infect MT-4 cells (2.5 × 10 ⁴ cells / well) with HIV and spread the infected cells immediately after infection with a microtiter plate with 96 wells containing various concentrations of the extract obtained in Preparation Example 2, and CO ₂ Incubate at 37 ° C. for 5 days in the incubator. The number of viable cells is measured by MTT method. Anti-HIV activity is expressed as a concentration that represents 50% protection against HIV-induced cell destruction by test substances. (EC 50: 50% effective concentration) The EC 50 is less than 21 ug / m1.

Experimental Example 4

WVOC (1 × 10 ⁶ cells / m1) extracted from gastric cancer was injected subcutaneously into 20 rats. From the next day, as an experimental group, 100 of these mice were injected daily at 100 mg / m1 of the extract obtained in Preparation 1.

Mice not administered the extract were used as controls. Cell growth is calculated for three weeks after administration of the extract. The number of cells in the control group is significantly increased while the increase of tumor cells in the experimental group is significantly suppressed.

Experimental Example 5

NVGC-4 (1 × 10 ⁶ cells / m 1) isolated from gastric cancer was injected subcutaneously into 20 rats. From the next day, as an experimental group, 10 of these rats are injected daily with the extract of 500 ug / mu obtained in Preparation Example 3. Mice not administered the extract are used as a control group. The growth of the cells after administration of the extract is calculated for 1 month. After one month, the cells in the control group increased markedly and there was some company area, and some animals died, but the number of cells in the experimental group was significantly suppressed, so that no animals died.

Experimental Example 6

NVGC-4 (1 × 10 ⁶ cells / mu) extracted from gastric cancer was loaded into a 96 well cyclator plate containing various concentrations of 75-subfraction obtained in Preparation Example 3, followed by CO ₂ incubator at 37 ° C. Incubate for 5 days at. The number of viable cells is measured by MTT method. The anticancer activity of unsaturated fatty acids and acidic polysaccharides is expressed as a concentration (IC 50: 50% effective concentration) that inhibits the growth of cancer cells by 50%. The 75-subfraction IC50DMS 200ug / m1, but the purified unsaturated fatty acids and acidic polysaccharides (25-75% sedimentation) do not inhibit tumor cell growth.

Claims (6)

Antiviral agent consisting of unsaturated fatty acid of sesame and alkaline synthesis of ganoderma lucidum as active ingredients The method of claim 1 Antiviral agent characterized in that the virus is a human immunodeficiency virus (HIV) The method of claim 1 Antiviral agent, wherein the unsaturated fatty acid solution is one of protein, amino acid, methionine, substrate, calcium component and alkaline solution is one of polysaccharide, organic acid, resin, mannitol, coumarin, lactone As an active ingredient Anticancer agent consisting of unsaturated fatty acid of sesame and alkaline synthetic extract of Ganoderma lucidum Method of producing antiviral agent by squeezing sesame without heating sesame and separating alkaline solution from hot water extraction of unsaturated fatty acid and Ganoderma lucidum The method of claim 4 Method for producing an anticancer agent, wherein the unsaturated fatty acid solution is one of protein, amino acid, methionine, substrate, calcium, alkaline solution is one of polysaccharide, organic acid, resin, mannitol, coumarin, lactone