CN107849572A - 用于可诱导共表达系统中的载体 - Google Patents

用于可诱导共表达系统中的载体 Download PDF

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Publication number
CN107849572A
CN107849572A CN201680035423.4A CN201680035423A CN107849572A CN 107849572 A CN107849572 A CN 107849572A CN 201680035423 A CN201680035423 A CN 201680035423A CN 107849572 A CN107849572 A CN 107849572A
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China
Prior art keywords
gene
promoter
host cell
expression
seq
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Pending
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CN201680035423.4A
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English (en)
Chinese (zh)
Inventor
S·麦克莱恩
M·维拉塞克
P·巴里施
J·民舒尔
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Abyssey
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Absci LLC
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Publication of CN107849572A publication Critical patent/CN107849572A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • General Factory Administration (AREA)
  • Preliminary Treatment Of Fibers (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Slot Machines And Peripheral Devices (AREA)
CN201680035423.4A 2015-06-16 2016-06-16 用于可诱导共表达系统中的载体 Pending CN107849572A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/740,475 2015-06-16
US14/740,475 US20150353940A1 (en) 2013-08-05 2015-06-16 Vectors for use in an inducible coexpression system
PCT/US2016/037942 WO2016205570A1 (en) 2015-06-16 2016-06-16 Vectors for use in an inducible coexpression system

Publications (1)

Publication Number Publication Date
CN107849572A true CN107849572A (zh) 2018-03-27

Family

ID=54769087

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680035423.4A Pending CN107849572A (zh) 2015-06-16 2016-06-16 用于可诱导共表达系统中的载体

Country Status (12)

Country Link
US (2) US20150353940A1 (enExample)
EP (2) EP4039810A1 (enExample)
JP (2) JP6976860B2 (enExample)
KR (1) KR102716963B1 (enExample)
CN (1) CN107849572A (enExample)
AU (2) AU2016278242B2 (enExample)
CA (1) CA2986443A1 (enExample)
DK (1) DK3310919T3 (enExample)
ES (1) ES2906725T3 (enExample)
IL (2) IL307555A (enExample)
MX (1) MX390195B (enExample)
WO (1) WO2016205570A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111269294A (zh) * 2018-12-05 2020-06-12 广州中国科学院先进技术研究所 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用
CN111979206A (zh) * 2019-05-24 2020-11-24 深圳瑞德林生物技术有限公司 固定化融合酶及用其制备谷胱甘肽的方法
CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技（广州）有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017106583A1 (en) * 2015-12-15 2017-06-22 Absci, Llc Cytoplasmic expression system
US12157766B2 (en) 2012-08-05 2024-12-03 Absci Corporation Cytoplasmic expression system
CA3114395A1 (en) 2018-09-25 2020-04-02 Absci, Llc Protein purification methods
KR20210090645A (ko) 2018-11-05 2021-07-20 제넨테크, 인크. 원핵 숙주 세포에서 2개의 사슬 단백질을 생산하는 방법
AU2021312935B2 (en) * 2020-07-24 2024-11-28 Duke University Methods and compositions for the production of acetyl-CoA derived products
US20220380783A1 (en) * 2021-05-06 2022-12-01 Arizona Board Of Regents On Behalf Of Arizona State University Methods and system of managing resource competition in synthetic gene circuits
WO2023114905A1 (en) 2021-12-16 2023-06-22 Absci Corporation Membrane-associated fusion proteins to increase the competency of cells
IL313521A (en) 2021-12-17 2024-08-01 Absci Corp Solid-phase screening for high-throughput bacterial strains
CN118510902A (zh) 2021-12-21 2024-08-16 Absci公司 用于鉴定具有增加的可溶性靶蛋白表达的菌株的FolA选择测定
EP4453198A1 (en) 2021-12-23 2024-10-30 ABSCI Corporation Products and methods for heterologous expression of proteins in a host cell
WO2023129881A1 (en) * 2021-12-30 2023-07-06 Absci Corporation Knockout of ptsp gene elevates active gene expression
US20230268026A1 (en) 2022-01-07 2023-08-24 Absci Corporation Designing biomolecule sequence variants with pre-specified attributes
WO2024006269A1 (en) 2022-06-29 2024-01-04 Absci Corporation Affinity screening method
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment
WO2024254237A1 (en) 2023-06-08 2024-12-12 Absci Corporation Solid-phase screening for high-performing bacterial strains
WO2025058962A1 (en) 2023-09-11 2025-03-20 Absci Corporation High-throughput methods for kinetic characterization, quantifying and optimizing antibodies and antibody fragments expression in bacteria
WO2025076081A1 (en) 2023-10-03 2025-04-10 Absci Corporation Tl1a associated antibody compositions and methods of use
WO2025122885A1 (en) 2023-12-08 2025-06-12 Absci Corporation Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use
WO2025144700A1 (en) 2023-12-27 2025-07-03 Absci Corporation Nanobody library screening using bacterial surface display

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1356052B1 (en) * 2000-12-14 2008-08-20 Genentech, Inc. Production of full antibodies in procaryotic cells
WO2015020690A1 (en) * 2013-08-05 2015-02-12 Aronora, Inc. Coordinated coexpression of thrombin
CN104603274A (zh) * 2012-08-05 2015-05-06 Absci有限责任公司 可诱导共表达系统

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3344618B2 (ja) 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド
ATE449844T1 (de) * 1997-12-05 2009-12-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
US6780405B1 (en) 2000-04-28 2004-08-24 Avant Immunotherapeutics, Inc. Regulated antigen delivery system (RADS)
US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
JP4461210B2 (ja) * 2001-08-27 2010-05-12 ジェネンテック， インコーポレイテッド 抗体発現系とその構築法
WO2005040388A2 (en) * 2003-08-22 2005-05-06 Nucleonics Inc. Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments
US8227241B2 (en) * 2004-03-12 2012-07-24 Unigene Laboratories, Inc. Bacterial host cell for the direct expression of peptides
DE102004061846A1 (de) * 2004-12-22 2006-07-13 Basf Ag Mehrfachpromotoren
CA2610405C (en) 2005-06-06 2015-01-27 Dow Global Technologies Inc. Mannitol induced promoter systems in bacterial host cells
US8178338B2 (en) 2005-07-01 2012-05-15 The Regents Of The University Of California Inducible expression vectors and methods of use thereof
EP2155789B1 (en) 2007-05-01 2013-07-24 Research Development Foundation Immunoglobulin fc libraries
US8999668B2 (en) 2008-01-03 2015-04-07 Cornell Research Foundation, Inc. Glycosylated protein expression in prokaryotes
KR100958096B1 (ko) * 2008-02-11 2010-05-14 한국과학기술원 염색체 특정 부위 제거용 재조합 벡터 및 이를 이용한미생물 내 염색체 특정 부위의 제거방법
DK2379722T3 (da) * 2008-12-16 2017-01-02 C-Lecta Gmbh Ekspressionsvektor
CA2754928A1 (en) * 2009-03-10 2010-09-16 Athena Biotechnologies, Inc. Reducing carbon dioxide production and increasing ethanol yield during microbial ethanol fermentation
US8623652B2 (en) * 2009-04-06 2014-01-07 Lucigen Corporation Host-vector system for cloning and expressing genes
AU2010322454B2 (en) * 2009-11-19 2016-05-19 Glaxosmithkline Biologicals S.A. Biosynthetic system that produces immunogenic polysaccharides in prokaryotic cells
US8603797B2 (en) * 2010-03-17 2013-12-10 Cornell University Methods and compositions for targeted mutagenesis in bacteria
EP2386641A1 (en) 2010-05-11 2011-11-16 Université Catholique De Louvain Method for extracellular production of recombinant biomolecules in a microorganism

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1356052B1 (en) * 2000-12-14 2008-08-20 Genentech, Inc. Production of full antibodies in procaryotic cells
CN104603274A (zh) * 2012-08-05 2015-05-06 Absci有限责任公司 可诱导共表达系统
WO2015020690A1 (en) * 2013-08-05 2015-02-12 Aronora, Inc. Coordinated coexpression of thrombin

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DAVID P HUMPHREYS: "A plasmid system for optimization of Fab" production in Escherichia coli: importance of balance of heavy chain and light chain synthesis", 《PROTEIN EXPRESSION AND PURIFICATION》 *
E M MCNALLY: "Coexpression and assembly of myosin heavy chain and myosin light chain in Escherichia coli", 《PNAS》 *
KYUNG-JIN KIM: "Two-promoter vector is highly efficient for overproduction of protein complexes", 《PROTEIN SCI》 *
梅毅: "携带IP-10基因双歧杆菌载体的构建及鉴定", 《广东医学》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111269294A (zh) * 2018-12-05 2020-06-12 广州中国科学院先进技术研究所 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用
CN111269294B (zh) * 2018-12-05 2023-03-28 广州中国科学院先进技术研究所 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用
CN111979206A (zh) * 2019-05-24 2020-11-24 深圳瑞德林生物技术有限公司 固定化融合酶及用其制备谷胱甘肽的方法
CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技（广州）有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用

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CA2986443A1 (en) 2016-12-22
EP3310919A1 (en) 2018-04-25
AU2022228166A1 (en) 2022-09-29
US20150353940A1 (en) 2015-12-10
JP2018518957A (ja) 2018-07-19
IL256094A (en) 2018-02-28
US20160376602A1 (en) 2016-12-29
EP3310919B1 (en) 2021-12-15
IL256094B1 (en) 2023-11-01
AU2016278242B2 (en) 2022-06-09
JP2021153600A (ja) 2021-10-07
IL307555A (en) 2023-12-01
HK1253045A1 (zh) 2019-06-06
KR20180020213A (ko) 2018-02-27
JP6976860B2 (ja) 2021-12-08
US11371048B2 (en) 2022-06-28
DK3310919T3 (da) 2022-02-14
EP3310919A4 (en) 2019-01-09
EP4039810A1 (en) 2022-08-10
ES2906725T3 (es) 2022-04-20
KR102716963B1 (ko) 2024-10-11
WO2016205570A1 (en) 2016-12-22
AU2016278242A1 (en) 2018-01-18
MX390195B (es) 2025-03-20
MX2017016400A (es) 2018-03-02
IL256094B2 (en) 2024-03-01

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