JP6976860B2 - 誘導性共発現系における使用のためのベクター - Google Patents

誘導性共発現系における使用のためのベクター Download PDF

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JP6976860B2
JP6976860B2 JP2017565149A JP2017565149A JP6976860B2 JP 6976860 B2 JP6976860 B2 JP 6976860B2 JP 2017565149 A JP2017565149 A JP 2017565149A JP 2017565149 A JP2017565149 A JP 2017565149A JP 6976860 B2 JP6976860 B2 JP 6976860B2
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expression
host cell
promoter
gene
inducible
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JP2018518957A (ja
JP2018518957A5 (enExample
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マクレイン，シーン
ヴァラセク，マーク
バリッシュ，フィリップ
ミンスハル，ジェレミー
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エイビーエスシーアイ・エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • General Factory Administration (AREA)
  • Preliminary Treatment Of Fibers (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Slot Machines And Peripheral Devices (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017565149A 2015-06-16 2016-06-16 誘導性共発現系における使用のためのベクター Active JP6976860B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021106797A JP2021153600A (ja) 2015-06-16 2021-06-28 誘導性共発現系における使用のためのベクター

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/740,475 2015-06-16
US14/740,475 US20150353940A1 (en) 2013-08-05 2015-06-16 Vectors for use in an inducible coexpression system
PCT/US2016/037942 WO2016205570A1 (en) 2015-06-16 2016-06-16 Vectors for use in an inducible coexpression system

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JP2021106797A Division JP2021153600A (ja) 2015-06-16 2021-06-28 誘導性共発現系における使用のためのベクター

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JP2018518957A JP2018518957A (ja) 2018-07-19
JP2018518957A5 JP2018518957A5 (enExample) 2019-07-25
JP6976860B2 true JP6976860B2 (ja) 2021-12-08

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JP2021106797A Pending JP2021153600A (ja) 2015-06-16 2021-06-28 誘導性共発現系における使用のためのベクター

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US (2) US20150353940A1 (enExample)
EP (2) EP4039810A1 (enExample)
JP (2) JP6976860B2 (enExample)
KR (1) KR102716963B1 (enExample)
CN (1) CN107849572A (enExample)
AU (2) AU2016278242B2 (enExample)
CA (1) CA2986443A1 (enExample)
DK (1) DK3310919T3 (enExample)
ES (1) ES2906725T3 (enExample)
IL (2) IL307555A (enExample)
MX (1) MX390195B (enExample)
WO (1) WO2016205570A1 (enExample)

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WO2017106583A1 (en) * 2015-12-15 2017-06-22 Absci, Llc Cytoplasmic expression system
US12157766B2 (en) 2012-08-05 2024-12-03 Absci Corporation Cytoplasmic expression system
CA3114395A1 (en) 2018-09-25 2020-04-02 Absci, Llc Protein purification methods
KR20210090645A (ko) 2018-11-05 2021-07-20 제넨테크, 인크. 원핵 숙주 세포에서 2개의 사슬 단백질을 생산하는 방법
CN111269294B (zh) * 2018-12-05 2023-03-28 广州中国科学院先进技术研究所 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用
CN111979206B (zh) * 2019-05-24 2021-08-17 深圳瑞德林生物技术有限公司 固定化融合酶及用其制备谷胱甘肽的方法
AU2021312935B2 (en) * 2020-07-24 2024-11-28 Duke University Methods and compositions for the production of acetyl-CoA derived products
US20220380783A1 (en) * 2021-05-06 2022-12-01 Arizona Board Of Regents On Behalf Of Arizona State University Methods and system of managing resource competition in synthetic gene circuits
CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技（广州）有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用
WO2023114905A1 (en) 2021-12-16 2023-06-22 Absci Corporation Membrane-associated fusion proteins to increase the competency of cells
IL313521A (en) 2021-12-17 2024-08-01 Absci Corp Solid-phase screening for high-throughput bacterial strains
CN118510902A (zh) 2021-12-21 2024-08-16 Absci公司 用于鉴定具有增加的可溶性靶蛋白表达的菌株的FolA选择测定
EP4453198A1 (en) 2021-12-23 2024-10-30 ABSCI Corporation Products and methods for heterologous expression of proteins in a host cell
WO2023129881A1 (en) * 2021-12-30 2023-07-06 Absci Corporation Knockout of ptsp gene elevates active gene expression
US20230268026A1 (en) 2022-01-07 2023-08-24 Absci Corporation Designing biomolecule sequence variants with pre-specified attributes
WO2024006269A1 (en) 2022-06-29 2024-01-04 Absci Corporation Affinity screening method
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment
WO2024254237A1 (en) 2023-06-08 2024-12-12 Absci Corporation Solid-phase screening for high-performing bacterial strains
WO2025058962A1 (en) 2023-09-11 2025-03-20 Absci Corporation High-throughput methods for kinetic characterization, quantifying and optimizing antibodies and antibody fragments expression in bacteria
WO2025076081A1 (en) 2023-10-03 2025-04-10 Absci Corporation Tl1a associated antibody compositions and methods of use
WO2025122885A1 (en) 2023-12-08 2025-06-12 Absci Corporation Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use
WO2025144700A1 (en) 2023-12-27 2025-07-03 Absci Corporation Nanobody library screening using bacterial surface display

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Publication number Publication date
CA2986443A1 (en) 2016-12-22
EP3310919A1 (en) 2018-04-25
AU2022228166A1 (en) 2022-09-29
US20150353940A1 (en) 2015-12-10
JP2018518957A (ja) 2018-07-19
IL256094A (en) 2018-02-28
US20160376602A1 (en) 2016-12-29
EP3310919B1 (en) 2021-12-15
IL256094B1 (en) 2023-11-01
AU2016278242B2 (en) 2022-06-09
JP2021153600A (ja) 2021-10-07
IL307555A (en) 2023-12-01
HK1253045A1 (zh) 2019-06-06
CN107849572A (zh) 2018-03-27
KR20180020213A (ko) 2018-02-27
US11371048B2 (en) 2022-06-28
DK3310919T3 (da) 2022-02-14
EP3310919A4 (en) 2019-01-09
EP4039810A1 (en) 2022-08-10
ES2906725T3 (es) 2022-04-20
KR102716963B1 (ko) 2024-10-11
WO2016205570A1 (en) 2016-12-22
AU2016278242A1 (en) 2018-01-18
MX390195B (es) 2025-03-20
MX2017016400A (es) 2018-03-02
IL256094B2 (en) 2024-03-01

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