AU2016278242B2 - Vectors for use in an inducible coexpression system - Google Patents

Vectors for use in an inducible coexpression system Download PDF

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Publication number
AU2016278242B2
AU2016278242B2 AU2016278242A AU2016278242A AU2016278242B2 AU 2016278242 B2 AU2016278242 B2 AU 2016278242B2 AU 2016278242 A AU2016278242 A AU 2016278242A AU 2016278242 A AU2016278242 A AU 2016278242A AU 2016278242 B2 AU2016278242 B2 AU 2016278242B2
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Australia
Prior art keywords
promoter
expression
inducible
gene
seq
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AU2016278242A
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English (en)
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AU2016278242A1 (en
Inventor
Philip BARISH
Sean Mcclain
Jeremy Minshull
Mark VALASEK
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Absci Corp
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Absci Corp
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Priority to AU2022228166A priority Critical patent/AU2022228166A1/en
Assigned to Absci Corporation reassignment Absci Corporation Request for Assignment Assignors: ABSCI, LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • General Factory Administration (AREA)
  • Preliminary Treatment Of Fibers (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Slot Machines And Peripheral Devices (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2016278242A 2015-06-16 2016-06-16 Vectors for use in an inducible coexpression system Active AU2016278242B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2022228166A AU2022228166A1 (en) 2015-06-16 2022-09-08 Vectors for use in an inducible coexpression system

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/740,475 2015-06-16
US14/740,475 US20150353940A1 (en) 2013-08-05 2015-06-16 Vectors for use in an inducible coexpression system
PCT/US2016/037942 WO2016205570A1 (en) 2015-06-16 2016-06-16 Vectors for use in an inducible coexpression system

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022228166A Division AU2022228166A1 (en) 2015-06-16 2022-09-08 Vectors for use in an inducible coexpression system

Publications (2)

Publication Number Publication Date
AU2016278242A1 AU2016278242A1 (en) 2018-01-18
AU2016278242B2 true AU2016278242B2 (en) 2022-06-09

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
AU2016278242A Active AU2016278242B2 (en) 2015-06-16 2016-06-16 Vectors for use in an inducible coexpression system
AU2022228166A Abandoned AU2022228166A1 (en) 2015-06-16 2022-09-08 Vectors for use in an inducible coexpression system

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2022228166A Abandoned AU2022228166A1 (en) 2015-06-16 2022-09-08 Vectors for use in an inducible coexpression system

Country Status (12)

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US (2) US20150353940A1 (enExample)
EP (2) EP4039810A1 (enExample)
JP (2) JP6976860B2 (enExample)
KR (1) KR102716963B1 (enExample)
CN (1) CN107849572A (enExample)
AU (2) AU2016278242B2 (enExample)
CA (1) CA2986443A1 (enExample)
DK (1) DK3310919T3 (enExample)
ES (1) ES2906725T3 (enExample)
IL (2) IL307555A (enExample)
MX (1) MX390195B (enExample)
WO (1) WO2016205570A1 (enExample)

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WO2017106583A1 (en) * 2015-12-15 2017-06-22 Absci, Llc Cytoplasmic expression system
US12157766B2 (en) 2012-08-05 2024-12-03 Absci Corporation Cytoplasmic expression system
CA3114395A1 (en) 2018-09-25 2020-04-02 Absci, Llc Protein purification methods
KR20210090645A (ko) 2018-11-05 2021-07-20 제넨테크, 인크. 원핵 숙주 세포에서 2개의 사슬 단백질을 생산하는 방법
CN111269294B (zh) * 2018-12-05 2023-03-28 广州中国科学院先进技术研究所 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用
CN111979206B (zh) * 2019-05-24 2021-08-17 深圳瑞德林生物技术有限公司 固定化融合酶及用其制备谷胱甘肽的方法
AU2021312935B2 (en) * 2020-07-24 2024-11-28 Duke University Methods and compositions for the production of acetyl-CoA derived products
US20220380783A1 (en) * 2021-05-06 2022-12-01 Arizona Board Of Regents On Behalf Of Arizona State University Methods and system of managing resource competition in synthetic gene circuits
CN113528410A (zh) * 2021-06-23 2021-10-22 云舟生物科技（广州）有限公司 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用
WO2023114905A1 (en) 2021-12-16 2023-06-22 Absci Corporation Membrane-associated fusion proteins to increase the competency of cells
IL313521A (en) 2021-12-17 2024-08-01 Absci Corp Solid-phase screening for high-throughput bacterial strains
CN118510902A (zh) 2021-12-21 2024-08-16 Absci公司 用于鉴定具有增加的可溶性靶蛋白表达的菌株的FolA选择测定
EP4453198A1 (en) 2021-12-23 2024-10-30 ABSCI Corporation Products and methods for heterologous expression of proteins in a host cell
WO2023129881A1 (en) * 2021-12-30 2023-07-06 Absci Corporation Knockout of ptsp gene elevates active gene expression
US20230268026A1 (en) 2022-01-07 2023-08-24 Absci Corporation Designing biomolecule sequence variants with pre-specified attributes
WO2024006269A1 (en) 2022-06-29 2024-01-04 Absci Corporation Affinity screening method
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment
WO2024254237A1 (en) 2023-06-08 2024-12-12 Absci Corporation Solid-phase screening for high-performing bacterial strains
WO2025058962A1 (en) 2023-09-11 2025-03-20 Absci Corporation High-throughput methods for kinetic characterization, quantifying and optimizing antibodies and antibody fragments expression in bacteria
WO2025076081A1 (en) 2023-10-03 2025-04-10 Absci Corporation Tl1a associated antibody compositions and methods of use
WO2025122885A1 (en) 2023-12-08 2025-06-12 Absci Corporation Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use
WO2025144700A1 (en) 2023-12-27 2025-07-03 Absci Corporation Nanobody library screening using bacterial surface display

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Also Published As

Publication number Publication date
CA2986443A1 (en) 2016-12-22
EP3310919A1 (en) 2018-04-25
AU2022228166A1 (en) 2022-09-29
US20150353940A1 (en) 2015-12-10
JP2018518957A (ja) 2018-07-19
IL256094A (en) 2018-02-28
US20160376602A1 (en) 2016-12-29
EP3310919B1 (en) 2021-12-15
IL256094B1 (en) 2023-11-01
JP2021153600A (ja) 2021-10-07
IL307555A (en) 2023-12-01
HK1253045A1 (zh) 2019-06-06
CN107849572A (zh) 2018-03-27
KR20180020213A (ko) 2018-02-27
JP6976860B2 (ja) 2021-12-08
US11371048B2 (en) 2022-06-28
DK3310919T3 (da) 2022-02-14
EP3310919A4 (en) 2019-01-09
EP4039810A1 (en) 2022-08-10
ES2906725T3 (es) 2022-04-20
KR102716963B1 (ko) 2024-10-11
WO2016205570A1 (en) 2016-12-22
AU2016278242A1 (en) 2018-01-18
MX390195B (es) 2025-03-20
MX2017016400A (es) 2018-03-02
IL256094B2 (en) 2024-03-01

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE INVENTOR TO READ MCCLAIN, SEAN; VALASEK, MARK; BARISH, PHILIP AND MINSHULL, JEREMY

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: ABSCI CORPORATION

Free format text: FORMER OWNER(S): ABSCI, LLC