CN107815465A - 人源化基因改造动物模型的制备方法及应用 - Google Patents
人源化基因改造动物模型的制备方法及应用 Download PDFInfo
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Abstract
Description
双链片段 | 1μL(0.5μM) |
pT7-sgRNA载体 | 1μL(10 ng) |
T4 DNA Ligase | 1μL(5U) |
10×T4 DNA Ligase buffer | 1μL |
50%PEG4000 | 1μL |
H2O | 补至10μL |
10×缓冲液 | 2μL |
dNTP(2mM) | 2μL |
MgSO4(25mM) | 0.8μL |
上游引物(10μM) | 0.6μL |
下游引物(10μM) | 0.6μL |
鼠尾基因组DNA | 200ng |
KOD-Plus-(1U/μL) | 0.6μL |
H2O | 补充到20μL |
2×Master Mix | 10μL |
上游引物(10μM) | 0.5μL |
下游引物(10μM) | 0.5μL |
鼠尾基因组DNA | 200ng |
KOD-Plus-(1U/μL) | 0.6μL |
ddH2O | 补至20μL |
Claims (37)
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EP17845421.1A EP3507374A4 (en) | 2016-08-31 | 2017-08-30 | GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERAL OX40 |
US16/329,269 US11279948B2 (en) | 2016-08-31 | 2017-08-30 | Genetically modified non-human animal with human or chimeric OX40 |
PCT/CN2017/099575 WO2018041119A1 (en) | 2016-08-31 | 2017-08-30 | Genetically modified non-human animal with human or chimeric ox40 |
US16/428,895 US11505806B2 (en) | 2016-08-31 | 2019-05-31 | Genetically modified non-human animal with human or chimeric OX40 |
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