CN107743826A - A kind of inoculation method of abalone mushroom culture medium - Google Patents
A kind of inoculation method of abalone mushroom culture medium Download PDFInfo
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- CN107743826A CN107743826A CN201711025439.XA CN201711025439A CN107743826A CN 107743826 A CN107743826 A CN 107743826A CN 201711025439 A CN201711025439 A CN 201711025439A CN 107743826 A CN107743826 A CN 107743826A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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Abstract
A kind of inoculation method of abalone mushroom culture medium, inoculation step are：（1）The compost for cultivating grey mushroom (abalonelike) is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby；（2）The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby；（3）By in the punching loop-carrier insertion abalone mushroom strains after sterilization, abalone mushroom strains are allowed to be attached in the strain groove of punching loop-carrier；（4）Punching loop-carrier with abalone mushroom strains is submerged into culture medium in linear insert from the face of culture medium, punching loop-carrier can not be taken out after insertion, allows it to stay in culture medium；（5）By the culture medium pile for being plugged punching loop-carrier on culturing rack, the culture medium of punching loop-carrier one side is inserted upward, you can complete inoculation；The raw material of described compost includes mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag, bagasse, agstone and blackstrap.This method inoculation efficiency is high and can reduce strain inoculation pollution probability.
Description
Technical field
The present invention relates to grey mushroom (abalonelike) technical field of cultivation, specifically a kind of inoculation method of abalone mushroom culture medium.
Background technology
Grey mushroom (abalonelike), Classification system：Pleurotusabalonus, alias TaiWan, China flat mushroom, mushroom is nutritious, meat fertilizer
Thickness, unique flavor contain 7.8 grams of protein in every 100 grams of dry products, 2.3 grams of fat, 10.2 grams of moisture, and 69 grams of polysaccharide is thick fine
5.6 grams of dimension, 21 milligrams of calcium, 220 milligrams of phosphorus, 3.2 milligrams of iron, 0.12 milligram of vitamin B1,7.09 milligrams of B2, niacin 6.7
Milligram, also containing 8 kinds of essential amino acids.And nonessential amino acid Glutamic Acid, lucid asparagus amino acid, alanine are most,
It is deep to be liked by locals.Grey mushroom (abalonelike) slightly warm in nature, it is sweet, there is nourishing, tonifying spleen and stomach, except warm heresy, wind dispelling, cold dispelling, relaxing tendons and activating collaterals
The effect of, lumbocrural pain, the grain not disease such as easypro, numb in every limb can be controlled.Modern medicine study proves, contains in abalone massee fruiting bodies
Antitumor cell polysaccharide body, the immunity of body can be improved, there is strong inhibitory action to tumour cell.Grey mushroom (abalonelike) is eaten for a long time,
There is the function of reducing blood pressure with cholesterol level, vascular sclerosis can be prevented, it is to hepatitis, gastritis and duodenal ulcer, soft stomach
Disease etc. has auxiliary therapeutic action.
In order to meet the market demand, people have invented the bacterium bag cultivation that can improve grey mushroom (abalonelike) yield, are to fill culture medium
Enter cultivating bag to obtain cultivating base rod, after being sterilized, then strain is seeded in and cultivated in base rod.In order to promote mycelia to cover with as early as possible
Bacterium bag, people start using card punch made of wood, iron staff, and it is circular cone to use and middle part is gone up made of plastics as cylinder, bottom
The inoculation hole fixer of body is punched temporarily in inoculation in bacterium bag, and strain is accessed in hollow hole.First punch and carry out strain again
It is high to be inoculated with pollution probability；The gap of strain and cultivating in a fungus bag base is big, and fruiting is irregular and inoculation efficiency is low.
The content of the invention
It is an object of the invention to provide a kind of inoculation method of abalone mushroom culture medium, this method inoculation efficiency is high and can reduce
Strain is inoculated with pollution probability.
The present invention is realized using following technical scheme：
A kind of inoculation method of abalone mushroom culture medium, inoculation step include：
（1）The compost for cultivating grey mushroom (abalonelike) is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby；
（2）The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby；
（3）By in the punching loop-carrier insertion abalone mushroom strains after sterilization, abalone mushroom strains are allowed to be attached to the strain of punching loop-carrier
In groove；
（4）Punching loop-carrier with abalone mushroom strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not
Punching loop-carrier can be taken out, allows it to stay in culture medium；Distance every 13-20cm inserts a punching loop-carrier；
（5）The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward,
It can complete to be inoculated with.
The preparation process of described culture medium is：
（1）Take by weight mulberry branch 20-30 parts, bamboo branch 10-20 parts, peanut shell 10-15 parts, Momordica grosvenori tendril 8-10 parts,
Corn ear slag 5-10 parts, bagasse 3-5 parts, agstone 3-5 parts and blackstrap 2-3 parts；
（2）Mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse powder are broken into less than 2 millimeters sizes
Particle；
（3）By the mulberry branch after crushing, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse and agstone and
Blackstrap is well mixed to obtain compound, then Mixed Microbes are added into compound, and adds water and be well mixed, and makes compound aqueous
Measure as 30-50%；The weight of compound and Mixed Microbes ratio is 100:（2-3）；Mixed Microbes are by bacillus subtilis and EM bacterium solution groups
Into the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5；
（4）After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days；Add limewash to stir, make to mix
It is 60-65% to close material water content, stands 2-3 days；Obtain compost；
（5）Compost is put into polybag, tightens sack；Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature
Spend for 100-120 DEG C；
（6）After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
Described limewash is that the water for precipitating and being filtrated to get is mixed using pulverized limestone and clear water；Pulverized limestone and clear water
Weight ratio is 1:（20-30）.
Described agstone is that the sieve that 80-100 mesh is crushed using lime stone is obtained.
Preferably：Described punching loop-carrier sterilization is placed in sterilizing pan, and boiling in pot is opened into sterilization 15-30 minutes.
The preparation process of described punching loop-carrier is：
（1）Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars；
（2）Cylindrical bars are truncated into the cylindrical section of more 10-12 centimeter lengths；
（3）Tip is made in cylindrical section both ends；
（4）By be made the cylindrical section both sides after tip make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle strains
Groove；The width of strain groove is 3-5 millimeters, and depth is 2-3 millimeters, and the spacing distance of strain groove is 2-3 millimeters；
（5）The cylindrical section for making strain groove is cut off from centre, you can obtain two punching loop-carriers.
Preferably：The strain groove of punching loop-carrier both sides is respectively 4-5.
Ramulus mori, Classification system：Morus alba L, be the branches and leaves of mulberry tree, ramulus mori, mulberry shoot, RAMULUS MORI, general name.Fallen leaves
Shrub or dungarunga, high 3-15m.Bark canescence, there is strip is shallow to split；Root skin yellowish-brown or reddish yellow, fibroid are strong.Single leaf is mutual
It is raw；The long l-2.5cm of petiole；Blade is avette or width egg shape, and long 5-20cm, wide 4-10cm, tip is sharp sharp or tapering, basal circular or
Near heart-shaped, there are rough sawn tooth or knuckle-tooth in edge, there is irregular division sometimes, above it is hairless, it is glossy, below have undercoat on arteries and veins,
Hairiness between armpit, base go out 3, arteries and veins and reticulated with thready pulse intertexture, and the back side is more apparent；Stipule lanceolar, it is caducous.
Corn ear slag is remaining slag after corn rod threshing.
Bagasse is the accessory substance of cane sugar factory, is all as fuel or paper making raw material all the time, causes resource
It is a large amount of to waste.The present invention uses bagasse to prepare White mushroom planting material for raw material, belongs to the item of turning waste into wealth of leftover bits and pieces recycling
Mesh, waste residue is not discharged externally, it is green；Contain substantial amounts of cellulose, the bagasse after everfermentation, crude fibre in bagasse
Can degrade, crude protein can improve, and its nutritive value is improved, formed White mushroom bacterium germination and growth needed for albumen and
Nutrient.
Described bacillus subtilis, Classification system：Bacillussubtilis, be bacillus one kind.Withered grass
0.7~0.8 × 2~3 microns of bacillus individual cells, uniform coloring.Without pod membrane, peritrichous, can move.Gram-positive
Bacterium, 0.6~0.9 × 1.0~1.5 microns of gemma, ellipse arrive column, central or slightly inclined positioned at thalline, and thalline is not after sporulation
Expand.Bacterium colony rough surface is opaque, dirty white or slightly yellow, and when being grown in liquid medium within, wrinkle mould is commonly formed.Withered grass bud
Spore bacillus has stronger protease, amylase and lipase active, can be active enzyme by internal activation of zymogen, can also divide
- serial other enzymes are secreted, such as pectase, dextranase, cellulase, can help to decompose the thing such as SNSP in plant feed
Matter, the present invention decompose the thick of pine tree branch, pine wood sawdust, mulberry branch, corn ear slag and bagasse using bacillus subtilis
Fiber, form albumen and nutrient of the grey mushroom (abalonelike) bacterium germination needed for growth.
EM bacterium（Effective Microorganisms）It is into EM bacterium are big by Japanese Ryukyu by about 80 kinds of microorganism groups
Ratio is good to be studied successfully professor's nineteen eighty-two according to husband, is put goods on the market the eighties.EM bacterium are with photosynthetic bacteria, lactic acid bacteria, yeast
A kind of micro- life bacteria preparation that more than 80 microorganisms of 10 category based on bacterium and actinomyces are combined.The mechanism of action is to form EM
Bacterium and the competition of pathogenic microorganism contention nutrition, because EM bacterium easily live and reproduce in soil, so can it is very fast and stably
Occupy the ecologic niche in soil, form the advantage group of beneficial microbial bacteria, so as to control the breeding of pathogenic microorganism and
Invasion and attack to crop.It is the developing direction of the ecological agriculture, is more beneficial for the sustainable development of agricultural.The beginning of the nineties at the end of the eighties,
EM bacterium by the states such as Japan, Thailand, Brazil, the U.S., Indonesia, Sri Lanka be widely used in agricultural, cultivation, plantation,
The fields such as environmental protection, achieve obvious economic benefit and ecological benefits.
Above-mentioned strain is cultivated to obtain by Guangxi Academy Of Sciences.
The present invention substantive distinguishing features and marked improvement be：
1st, the inoculation method of this abalone mushroom culture medium is inserted in abalone mushroom strains using special punching loop-carrier, abalone mushroom strains
It is attached in the strain groove of punching loop-carrier, the punching loop-carrier with abalone mushroom strains is inserted not from the face of culture medium in linear
Enter culture medium, punching loop-carrier can not be taken out after insertion, allows it to stay in culture medium；Strain can be planted quickly in culture medium,
The contaminated probability of strain is reduced, and strain is combined closely with cultivating in a fungus bag base, the strain kind per cave enters amount equilibrium, plants into depth
Also it is balanced, reduce the irregular probability of fruiting；And inoculation time is that tradition first punches 3/5ths of the inoculation time being inoculated with afterwards.
2nd, mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse powder are broken into 2 millimeters by the application
The particle of following size；By the mulberry branch after crushing, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse and stone
Limestone flour and blackstrap, which are well mixed, obtains compound, then Mixed Microbes are added into compound, and adds water and be well mixed, and makes to mix
It is 30-50% to close material water content；The weight of compound and Mixed Microbes ratio is 100:（2-3）；Mixed Microbes are by bacillus subtilis and EM
Bacterium solution forms, and the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5；The compound for adding Mixed Microbes is placed on Indoor Natural
After fermentation 5-7 days；Add limewash to stir, it is 60-65% to make mixture moisture content, stands 2-3 days；Obtain compost；
Compost is put into polybag, tightens sack；Pile is put into steam sterilizing pan, sterilizes 6-10 hours, and vapor (steam) temperature is
100-120℃；After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.Obtain
Culture medium be adapted to grey mushroom (abalonelike) growth, ensure cultivating rate, and fruiting is fast, and mycelia is non-aging.Culture medium utilizes bacillus subtilis
Bacterium, EM strains and pulverized limestone fermented, and improved the disease-resistant and anti-miscellaneous bacteria infection ability of grey mushroom (abalonelike).
Brief description of the drawings
Fig. 1 is the structural representation of cylindrical section；
Fig. 2 is the structural representation of cylindrical section strain groove；
Fig. 3 is Fig. 2 left view；
Fig. 4 is the structural representation of punching loop-carrier；
Sequence number is entitled in figure：
1st, cylindrical section；2nd, tip；3rd, strain groove；4th, loop-carrier is punched.
Embodiment
With reference to embodiment, the technical scheme in invention is clearly and completely described, described embodiment is only
Only it is the part of the present invention, rather than whole embodiments.
Embodiment 1
The inoculation of abalone mushroom culture medium can be completed using following processing step：
（1）The compost for cultivating grey mushroom (abalonelike) is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby；
（2）The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is placed in sterilizing pan, boiling in pot is opened
15-30 minutes are sterilized, it is standby to take out natural cooling；
（3）By in the punching loop-carrier insertion abalone mushroom strains after sterilization, abalone mushroom strains are allowed to be attached to the strain of punching loop-carrier
In groove；
（4）Punching loop-carrier with abalone mushroom strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not
Punching loop-carrier can be taken out, allows it to stay in culture medium；Distance every 10-20cm inserts a punching loop-carrier；
（5）The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward,
It can complete to be inoculated with.
The preparation process of described culture medium is：
（1）Take by weight mulberry branch 20-30 parts, bamboo branch 10-20 parts, peanut shell 10-15 parts, Momordica grosvenori tendril 8-10 parts,
Corn ear slag 5-10 parts, bagasse 3-5 parts, agstone 3-5 parts and blackstrap 2-3 parts；Described agstone is to utilize stone
The sieve that lime stone crushed 80-100 mesh obtains；
（2）Mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse powder are broken into less than 2 millimeters sizes
Particle；
（3）By the mulberry branch after crushing, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse and agstone and
Blackstrap is well mixed to obtain compound, then Mixed Microbes are added into compound, and adds water and be well mixed, and makes compound aqueous
Measure as 30-50%；The weight of compound and Mixed Microbes ratio is 100:（2-3）；Mixed Microbes are by bacillus subtilis and EM bacterium solution groups
Into the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5；
（4）After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days；Add limewash to stir, make to mix
It is 60-65% to close material water content, stands 2-3 days；Obtain compost；Described limewash is to mix to stir with clear water using pulverized limestone
Mix the water that precipitation is filtrated to get；The weight of pulverized limestone and clear water ratio is 1:（20-30）；
（5）Compost is put into polybag, tightens sack；Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature
Spend for 100-120 DEG C；
（6）After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
The preparation process of described punching loop-carrier is：
（1）Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars；
（2）Cylindrical bars are truncated into the cylindrical section 1 of more 10-12 centimeter lengths；
（3）Tip 2 is made in the both ends of cylindrical section 1；
（4）By be made the both sides of cylindrical section 1 after tip 2 make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle bacterium
Kind groove 3；The width of strain groove 3 is 3-5 millimeters, and depth is 2-3 millimeters；
（5）The cylindrical section 1 for making strain groove 3 is cut off from centre, you can obtain two punching loop-carriers 4；Ensure punching inoculation
The strain groove of rod both sides is respectively 5.
Application Example
1st, Jinxiu Yao ethnic group in Guangxi province autonomous county Huang, original grey mushroom (abalonelike) of planting first is struck with a stick behind cave in inoculation, artificial
Strain is filled in cave, a people can be inoculated with 20 bag culture mediums in one hour；The neat rate of fruiting is less than 70%；Later Huang's profit
With in the punching loop-carrier insertion abalone mushroom strains of the present invention, abalone mushroom strains are allowed to be attached in the strain groove of punching loop-carrier；Will
Punching loop-carrier with abalone mushroom strains submerges culture medium from the face of culture medium in linear insert, and punching can not be taken out after insertion
Loop-carrier, it is allowed to stay in culture medium；One people can be inoculated with more than 32 bags per hour；The neat rate of fruiting is more than 95%；Through statistics
Daily fruiting amount can receive 3 days mushrooms more than first beating behind cave 2.3% more than the fruiting amount being inoculated with.
Described above is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art it is general
Logical technical staff, in the essential scope of the present invention, the variations, modifications, additions or substitutions made, it should all belong to the guarantor of the present invention
Protect scope.
Claims (7)
- A kind of 1. inoculation method of abalone mushroom culture medium, it is characterised in that：Inoculation step includes：（1）The compost for cultivating grey mushroom (abalonelike) is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby；（2）The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby；（3）By in the punching loop-carrier insertion abalone mushroom strains after sterilization, abalone mushroom strains are allowed to be attached to the strain of punching loop-carrier In groove；（4）Punching loop-carrier with abalone mushroom strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not Punching loop-carrier can be taken out, allows it to stay in culture medium；Distance every 10-20cm inserts a punching loop-carrier；（5）The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward, It can complete to be inoculated with；The raw material of described compost includes mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag, bagasse, stone Limestone flour and blackstrap.
- 2. the inoculation method of abalone mushroom culture medium according to claim 1, it is characterised in that：The preparation of described culture medium Process is：（1）Take by weight mulberry branch 20-30 parts, bamboo branch 10-20 parts, peanut shell 10-15 parts, Momordica grosvenori tendril 8-10 parts, Corn ear slag 5-10 parts, bagasse 3-5 parts, agstone 3-5 parts and blackstrap 2-3 parts；（2）Mulberry branch, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse powder are broken into less than 2 millimeters sizes Particle；（3）By the mulberry branch after crushing, bamboo branch, peanut shell, Momordica grosvenori tendril, corn ear slag and bagasse and agstone and Blackstrap is well mixed to obtain compound, then Mixed Microbes are added into compound, and adds water and be well mixed, and makes compound aqueous Measure as 30-50%；The weight of compound and Mixed Microbes ratio is 100:（2-3）；Mixed Microbes are by bacillus subtilis and EM bacterium solution groups Into the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5；（4）After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days；Add limewash to stir, make to mix It is 60-65% to close material water content, stands 2-3 days；Obtain compost；（5）Compost is put into polybag, tightens sack；Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature Spend for 100-120 DEG C；（6）After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
- 3. the inoculation method of abalone mushroom culture medium according to claim 1, it is characterised in that：Described punching loop-carrier disappears Poison is placed in sterilizing pan, and boiling in pot is opened into sterilization 15-30 minutes.
- 4. the inoculation method of abalone mushroom culture medium according to claim 2, it is characterised in that：Described limewash is to utilize Pulverized limestone mixes with clear water and precipitates the water being filtrated to get；The weight of pulverized limestone and clear water ratio is 1:（20-30）.
- 5. the inoculation method of abalone mushroom culture medium according to claim 1, it is characterised in that：Described agstone is profit The sieve that 80-100 mesh is crushed with lime stone obtains.
- 6. the inoculation method of abalone mushroom culture medium according to claim 1, it is characterised in that：Described punching loop-carrier Preparation process is：（1）Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars；（2）Cylindrical bars are truncated into the cylindrical section of more 10-12 centimeter lengths（1）；（3）By cylindrical section（1）Tip is made in both ends（2）；（4）Tip will be made（2）Cylindrical section afterwards（1）Both sides make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle Strain groove（3）；Strain groove（3）Width be 3-5 millimeters, depth is 2-3 millimeters, strain groove（3）Spacing distance for 2-3 milli Rice；（5）Strain groove will be made（3）Cylindrical section（1）Cut off from centre, you can obtain two punching loop-carriers（4）.
- 7. the inoculation method of abalone mushroom culture medium according to claim 1, it is characterised in that：Punch the bacterium of loop-carrier both sides Kind groove is respectively 4-5.
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| CN105315072A (en) * | 2015-11-04 | 2016-02-10 | 苏州市经纬农产品有限公司 | Culture medium for culturing abalone mushroom |
| CN105347923A (en) * | 2015-11-04 | 2016-02-24 | 苏州市经纬农产品有限公司 | Abalone mushroom culture medium and preparation method thereof |
| CN106034742A (en) * | 2016-06-23 | 2016-10-26 | 广西南宁北部湾现代农业有限公司 | Method for producing big Clitocybe by using mulberry stems, sugarcane bagasse and silkworm excrement |
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2017
- 2017-10-27 CN CN201711025439.XA patent/CN107743826B/en active Active
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|---|---|---|---|---|
| CN1057752A (en) * | 1990-07-01 | 1992-01-15 | 汤金阶 | Food (medicine) a kind of segment wood cultivated method of bacterium |
| CN102668876A (en) * | 2012-04-19 | 2012-09-19 | 山西省农业科学院试验研究中心 | Method for manufacturing lengthened mushroom cultivating stick |
| CN203152081U (en) * | 2013-04-16 | 2013-08-28 | 赵李华 | Edible mushroom inoculation nail |
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| CN107743826B (en) | 2020-10-16 |
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