CN107646535A - A kind of inoculation method of pleurotus eryngii culture medium - Google Patents

A kind of inoculation method of pleurotus eryngii culture medium Download PDF

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CN107646535A
CN107646535A CN201711029859.5A CN201711029859A CN107646535A CN 107646535 A CN107646535 A CN 107646535A CN 201711029859 A CN201711029859 A CN 201711029859A CN 107646535 A CN107646535 A CN 107646535A
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culture medium
carrier
pleurotus eryngii
branch
loop
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CN107646535B (en
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黄兴根
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Heilongjiang Bingrong Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/10Addition or removal of substances other than water or air to or from the material during the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Mechanical Engineering (AREA)
  • Mushroom Cultivation (AREA)

Abstract

A kind of inoculation method of pleurotus eryngii culture medium, inoculation step are:(1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;(2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby;(3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached in the strain groove of punching loop-carrier;(4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium in linear insert from the face of culture medium, punching loop-carrier can not be taken out after insertion, allows it to stay in culture medium;Distance every 8 15cm inserts a punching loop-carrier;(5)By the culture medium pile for being plugged punching loop-carrier on culturing rack, the culture medium of punching loop-carrier one side is inserted upward, you can;The raw material of described compost includes Chinese fir branch, China fir sawdust, fernwort, mulberry branch, bagasse, blackstrap and pulverized limestone;This method inoculation efficiency is high and can reduce strain inoculation pollution probability.

Description

A kind of inoculation method of pleurotus eryngii culture medium
Technical field
The present invention relates to planting almond abalone mushroom technical field, specifically a kind of inoculation method of pleurotus eryngii culture medium.
Background technology
Pleurotus eryngii, scientific name:Pleurotus eryngii Quel., also known as pleurotus eryngii, because its have almond fragrance and Bacterial context is plump to gain the name such as the mouthfeel of abalone.It is that exploitation in recent years cultivates successful and edible, medicinal, dietotherapy in the rare of one Edible mushroom new varieties.Pleurotus eryngii bacterial context is plump, and quality is tender and crisp, particularly stem dense structure, solid, milky white, can all eat, And it is stem cunning more crisp than cap, tasty and refreshing, it is referred to as " flat mushroom king ", " dried scallop mushroom ", there is happy almond flavor and such as abalone Mouthfeel, it is adapted to fresh-keeping, processing, firmly gets liking for people.
In order to meet the market demand, people have invented the bacterium bag cultivation that can improve pleurotus eryngii yield, are to fill culture medium Enter cultivating bag to obtain cultivating base rod, after being sterilized, then strain is seeded in and cultivated in base rod.In order to promote mycelia to cover with as early as possible Bacterium bag, people start using card punch made of wood, iron staff, and it is circular cone to use and middle part is gone up made of plastics as cylinder, bottom The inoculation hole fixer of body is punched temporarily in inoculation in bacterium bag, and strain is accessed in hollow hole.First punch and carry out strain again It is high to be inoculated with pollution probability;The gap of strain and cultivating in a fungus bag base is big, and fruiting is irregular and inoculation efficiency is low.
The content of the invention
It is an object of the invention to provide a kind of inoculation method of pleurotus eryngii culture medium, this method inoculation efficiency is high and can reduce Strain is inoculated with pollution probability.
The present invention is realized using following technical scheme:
A kind of inoculation method of pleurotus eryngii culture medium, inoculation step include:
(1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;
(2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby;
(3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached to the strain of punching loop-carrier In groove;
(4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not Punching loop-carrier can be taken out, allows it to stay in culture medium;Distance every 10-15cm inserts a punching loop-carrier;
(5)The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward, It can complete to be inoculated with.
The preparation process of described culture medium is:
(1)Golden peach ma branch 20-35 parts, mulberry branch 10-20 parts, anistree branch 10-15 parts, peanut shell 8-10 are taken by weight Part, corn ear slag 5-8 parts, bagasse 3-5 parts, blackstrap 2-3 parts and pulverized limestone 1-1.5 parts;
(2)Golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into less than 2 millimeters The particle of size;
(3)By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse and blackstrap It is well mixed to obtain compound, then Mixed Microbes are added into compound, and add water and be well mixed, make the mixture moisture content be 50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are made up of bacillus subtilis and EM bacterium solutions, withered The weight of careless bacillus and EM bacterium solutions ratio is 1:1.5;
(4)After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days;It is uniform to add lime powder and stirring, stands 2-3 days;Obtain compost;
(5)Compost is put into polybag, tightens sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature Spend for 100-120 DEG C;
(6)After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
Preferably:Described punching loop-carrier sterilization is placed in sterilizing pan, and boiling in pot is opened into sterilization 15-30 minutes.
The preparation process of described punching loop-carrier is:
(1)Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars;
(2)Cylindrical bars are truncated into the cylindrical section of more 10-12 centimeter lengths;
(3)Tip is made in cylindrical section both ends;
(4)By be made the cylindrical section both sides after tip make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle strains Groove;The width of strain groove is 3-5 millimeters, and depth is 2-3 millimeters, and the spacing distance of strain groove is 2-3 millimeters;
(5)The cylindrical section for making strain groove is cut off from centre, you can obtain two punching loop-carriers.
Preferably:The strain groove of punching loop-carrier both sides is respectively 4-5.
Ramulus mori, Classification system:Morus alba L, be the branches and leaves of mulberry tree, ramulus mori, mulberry shoot, RAMULUS MORI, general name.Fallen leaves Shrub or dungarunga, high 3-15m.Bark canescence, there is strip is shallow to split;Root skin yellowish-brown or reddish yellow, fibroid are strong.Single leaf is mutual It is raw;The long l-2.5cm of petiole;Blade is avette or width egg shape, and long 5-20cm, wide 4-10cm, tip is sharp sharp or tapering, basal circular or Near heart-shaped, there are rough sawn tooth or knuckle-tooth in edge, there is irregular division sometimes, above it is hairless, it is glossy, below have undercoat on arteries and veins, Hairiness between armpit, base go out 3, arteries and veins and reticulated with thready pulse intertexture, and the back side is more apparent;Stipule lanceolar, it is caducous.
Corn ear slag is remaining slag after corn rod threshing.
Bagasse is the accessory substance of cane sugar factory, is all as fuel or paper making raw material all the time, causes resource It is a large amount of to waste.The present invention uses bagasse to prepare White mushroom planting material for raw material, belongs to the item of turning waste into wealth of leftover bits and pieces recycling Mesh, waste residue is not discharged externally, it is green;Contain substantial amounts of cellulose, the bagasse after everfermentation, crude fibre in bagasse Can degrade, crude protein can improve, and its nutritive value is improved, formed White mushroom bacterium germination and growth needed for albumen and Nutrient.
Described bacillus subtilis, Classification system:Bacillussubtilis, be bacillus one kind.Withered grass 0.7~0.8 × 2~3 microns of bacillus individual cells, uniform coloring.Without pod membrane, peritrichous, can move.Gram-positive Bacterium, 0.6~0.9 × 1.0~1.5 microns of gemma, ellipse arrive column, central or slightly inclined positioned at thalline, and thalline is not after sporulation Expand.Bacterium colony rough surface is opaque, dirty white or slightly yellow, and when being grown in liquid medium within, wrinkle mould is commonly formed.Withered grass bud Spore bacillus has stronger protease, amylase and lipase active, can be active enzyme by internal activation of zymogen, can also divide - serial other enzymes are secreted, such as pectase, dextranase, cellulase, can help to decompose the thing such as SNSP in plant feed Matter, the present invention decompose the thick of pine tree branch, pine wood sawdust, mulberry branch, corn ear slag and bagasse using bacillus subtilis Fiber, form albumen and nutrient of the pleurotus eryngii bacterium germination needed for growth.
EM bacterium(Effective Microorganisms)It is into EM bacterium are big by Japanese Ryukyu by about 80 kinds of microorganism groups Ratio is good to be studied successfully professor's nineteen eighty-two according to husband, is put goods on the market the eighties.EM bacterium are with photosynthetic bacteria, lactic acid bacteria, yeast A kind of micro- life bacteria preparation that more than 80 microorganisms of 10 category based on bacterium and actinomyces are combined.The mechanism of action is to form EM Bacterium and the competition of pathogenic microorganism contention nutrition, because EM bacterium easily live and reproduce in soil, so can it is very fast and stably Occupy the ecologic niche in soil, form the advantage group of beneficial microbial bacteria, so as to control the breeding of pathogenic microorganism and Invasion and attack to crop.It is the developing direction of the ecological agriculture, is more beneficial for the sustainable development of agricultural.The beginning of the nineties at the end of the eighties, EM bacterium by the states such as Japan, Thailand, Brazil, the U.S., Indonesia, Sri Lanka be widely used in agricultural, cultivation, plantation, The fields such as environmental protection, achieve obvious economic benefit and ecological benefits.
Above-mentioned strain is cultivated to obtain by Guangxi Academy Of Sciences.
The present invention substantive distinguishing features and marked improvement be:
1st, the inoculation method of this pleurotus eryngii culture medium is inserted in pleurotus eryngii quel strains using special punching loop-carrier, pleurotus eryngii quel strains It is attached in the strain groove of punching loop-carrier, the punching loop-carrier with pleurotus eryngii quel strains is inserted not from the face of culture medium in linear Enter culture medium, punching loop-carrier can not be taken out after insertion, allows it to stay in culture medium;Strain can be planted quickly in culture medium, The contaminated probability of strain is reduced, and strain is combined closely with cultivating in a fungus bag base, the strain kind per cave enters amount equilibrium, plants into depth Also it is balanced, reduce the irregular probability of fruiting;And inoculation time is that tradition first punches 3/5ths of the inoculation time being inoculated with afterwards.
2nd, golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into 2 by the application The particle of the following size of millimeter;By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and sweet Bagasse is well mixed to obtain compound with blackstrap, then Mixed Microbes are added into compound, and is added water and be well mixed, and makes mixing Material water content is 50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are by bacillus subtilis and EM bacterium Liquid forms, and the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5;The compound for adding Mixed Microbes is placed on Indoor Natural hair After ferment 5-7 days;It is uniform to add lime powder and stirring, stands 2-3 days;Obtain compost;Compost is put into polybag, tightened Sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, and vapor (steam) temperature is 100-120 DEG C;After sterilization terminates, it will be equipped with The polybag of compost takes out, and naturally cools to normal temperature, produces culture medium.Obtained culture medium is adapted to Growth of Pleurotus eryngii, ensures Cultivating rate, and fruiting is fast, mycelia is non-aging.Culture medium was fermented using bacillus subtilis, EM strains and pulverized limestone, was improved The disease-resistant and anti-miscellaneous bacteria infection ability of pleurotus eryngii.
Brief description of the drawings
Fig. 1 is the structural representation of cylindrical section;
Fig. 2 is the structural representation of cylindrical section strain groove;
Fig. 3 is Fig. 2 left view;
Fig. 4 is the structural representation of punching loop-carrier;
Sequence number is entitled in figure:
1st, cylindrical section;2nd, tip;3rd, strain groove;4th, loop-carrier is punched.
Embodiment
With reference to embodiment, the technical scheme in invention is clearly and completely described, described embodiment is only Only it is the part of the present invention, rather than whole embodiments.
Embodiment 1
The inoculation of pleurotus eryngii culture medium can be completed using following processing step:
(1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;
(2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is placed in sterilizing pan, boiling in pot is opened 15-30 minutes are sterilized, it is standby to take out natural cooling;
(3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached to the strain of punching loop-carrier In groove;
(4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not Punching loop-carrier can be taken out, allows it to stay in culture medium;Distance every 10-15cm inserts a punching loop-carrier;
(5)The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward, It can complete to be inoculated with.
The preparation process of described culture medium is:
(1)Golden peach ma branch 20-35 parts, mulberry branch 10-20 parts, anistree branch 10-15 parts, peanut shell 8-10 are taken by weight Part, corn ear slag 5-8 parts, bagasse 3-5 parts, blackstrap 2-3 parts and pulverized limestone 1-1.5 parts;
(2)Golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into less than 2 millimeters The particle of size;
(3)By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse and blackstrap It is well mixed to obtain compound, then Mixed Microbes are added into compound, and add water and be well mixed, make the mixture moisture content be 50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are made up of bacillus subtilis and EM bacterium solutions, withered The weight of careless bacillus and EM bacterium solutions ratio is 1:1.5;
(4)After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days;It is uniform to add lime powder and stirring, stands 2-3 days;Obtain compost;
(5)Compost is put into polybag, tightens sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature Spend for 100-120 DEG C;
(6)After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
The preparation process of described punching loop-carrier is:
(1)Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars;
(2)Cylindrical bars are truncated into the cylindrical section 1 of more 10-12 centimeter lengths;
(3)Tip 2 is made in the both ends of cylindrical section 1;
(4)By be made the both sides of cylindrical section 1 after tip 2 make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle bacterium Kind groove 3;The width of strain groove 3 is 3-5 millimeters, and depth is 2-3 millimeters;
(5)The cylindrical section 1 for making strain groove 3 is cut off from centre, you can obtain two punching loop-carriers 4;Ensure punching inoculation The strain groove of rod both sides is respectively 5.
Application Example
1st, Jinxiu Yao ethnic group in Guangxi province autonomous county Huang, original planting pleurotus eryngii in inoculation is first struck with a stick behind cave, artificial Strain is filled in cave, a people can be inoculated with 22 bag culture mediums in one hour;The neat rate of fruiting is less than 75%;Later Huang's profit With in the punching loop-carrier insertion pleurotus eryngii quel strains of the present invention, pleurotus eryngii quel strains are allowed to be attached in the strain groove of punching loop-carrier;Will Punching loop-carrier with pleurotus eryngii quel strains submerges culture medium from the face of culture medium in linear insert, and punching can not be taken out after insertion Loop-carrier, it is allowed to stay in culture medium;One people can be inoculated with more than 36 bags per hour;The neat rate of fruiting is more than 93%;Through statistics Daily fruiting amount can receive 3 days mushrooms more than first beating behind cave 2.5% more than the fruiting amount being inoculated with.
Described above is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art it is general Logical technical staff, in the essential scope of the present invention, the variations, modifications, additions or substitutions made, it should all belong to the guarantor of the present invention Protect scope.

Claims (5)

  1. A kind of 1. inoculation method of pleurotus eryngii culture medium, it is characterised in that:Inoculation step includes:
    (1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;
    (2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby;
    (3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached to the strain of punching loop-carrier In groove;
    (4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not Punching loop-carrier can be taken out, allows it to stay in culture medium;Distance every 10-15cm inserts a punching loop-carrier;
    (5)The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward, It can complete to be inoculated with;
    The raw material of described compost includes golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag, sugarcane Slag, blackstrap and pulverized limestone.
  2. 2. the inoculation method of pleurotus eryngii culture medium according to claim 1, it is characterised in that:The preparation of described culture medium Process is:
    (1)Golden peach ma branch 20-35 parts, mulberry branch 10-20 parts, anistree branch 10-15 parts, peanut shell 8-10 are taken by weight Part, corn ear slag 5-8 parts, bagasse 3-5 parts, blackstrap 2-3 parts and pulverized limestone 1-1.5 parts;
    (2)Golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into less than 2 millimeters The particle of size;
    (3)By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse and blackstrap It is well mixed to obtain compound, then Mixed Microbes are added into compound, and add water and be well mixed, make the mixture moisture content be 50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are made up of bacillus subtilis and EM bacterium solutions, withered The weight of careless bacillus and EM bacterium solutions ratio is 1:1.5;
    (4)After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days;It is uniform to add lime powder and stirring, stands 2-3 days;Obtain compost;
    (5)Compost is put into polybag, tightens sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature Spend for 100-120 DEG C;
    (6)After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
  3. 3. the inoculation method of pleurotus eryngii culture medium according to claim 1, it is characterised in that:Described punching loop-carrier disappears Poison is placed in sterilizing pan, and boiling in pot is opened into sterilization 15-30 minutes.
  4. 4. the inoculation method of pleurotus eryngii culture medium according to claim 1, it is characterised in that:Described punching loop-carrier Preparation process is:
    (1)Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars;
    (2)Cylindrical bars are truncated into the cylindrical section of more 10-12 centimeter lengths(1);
    (3)By cylindrical section(1)Tip is made in both ends(2);
    (4)Tip will be made(2)Cylindrical section afterwards(1)Both sides make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle Strain groove(3);Strain groove(3)Width be 3-5 millimeters, depth is 2-3 millimeters, strain groove(3)Spacing distance for 2-3 milli Rice;
    (5)Strain groove will be made(3)Cylindrical section(1)Cut off from centre, you can obtain two punching loop-carriers(4).
  5. 5. the inoculation method of pleurotus eryngii culture medium according to claim 1, it is characterised in that:Punch the bacterium of loop-carrier both sides Kind groove is respectively 4-5.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109122035A (en) * 2018-11-07 2019-01-04 广西仁泰生物科技有限公司 A kind of implantation methods of tea tree interplanting Pleurotus eryngii
CN111615995A (en) * 2020-07-08 2020-09-04 贵州贵旺生物科技有限公司 Culture medium for liquid fermentation of pleurotus eryngii
CN111642326A (en) * 2020-07-08 2020-09-11 贵州贵旺生物科技有限公司 Mother culture method of pleurotus eryngii liquid strain

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CN1057752A (en) * 1990-07-01 1992-01-15 汤金阶 Food (medicine) a kind of segment wood cultivated method of bacterium
CN102668876A (en) * 2012-04-19 2012-09-19 山西省农业科学院试验研究中心 Method for manufacturing lengthened mushroom cultivating stick
CN203152081U (en) * 2013-04-16 2013-08-28 赵李华 Edible mushroom inoculation nail
CN104663240A (en) * 2013-11-28 2015-06-03 北京世纪阿姆斯生物技术股份有限公司 Pleurotus eryngii culture method and preparation method for microbial fertilizer by pleurotus eryngii
CN105087394A (en) * 2015-08-28 2015-11-25 襄汾县侯临农业科技有限公司 Pleurotus eryngii mushroom strain production method
CN106034742A (en) * 2016-06-23 2016-10-26 广西南宁北部湾现代农业有限公司 Method for producing big Clitocybe by using mulberry stems, sugarcane bagasse and silkworm excrement

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CN109122035A (en) * 2018-11-07 2019-01-04 广西仁泰生物科技有限公司 A kind of implantation methods of tea tree interplanting Pleurotus eryngii
CN111615995A (en) * 2020-07-08 2020-09-04 贵州贵旺生物科技有限公司 Culture medium for liquid fermentation of pleurotus eryngii
CN111642326A (en) * 2020-07-08 2020-09-11 贵州贵旺生物科技有限公司 Mother culture method of pleurotus eryngii liquid strain

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