The content of the invention
It is an object of the invention to provide a kind of inoculation method of pleurotus eryngii culture medium, this method inoculation efficiency is high and can reduce
Strain is inoculated with pollution probability.
The present invention is realized using following technical scheme:
A kind of inoculation method of pleurotus eryngii culture medium, inoculation step include:
(1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;
(2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is sterilized, it is standby;
(3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached to the strain of punching loop-carrier
In groove;
(4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not
Punching loop-carrier can be taken out, allows it to stay in culture medium;Distance every 10-15cm inserts a punching loop-carrier;
(5)The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward,
It can complete to be inoculated with.
The preparation process of described culture medium is:
(1)Golden peach ma branch 20-35 parts, mulberry branch 10-20 parts, anistree branch 10-15 parts, peanut shell 8-10 are taken by weight
Part, corn ear slag 5-8 parts, bagasse 3-5 parts, blackstrap 2-3 parts and pulverized limestone 1-1.5 parts;
(2)Golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into less than 2 millimeters
The particle of size;
(3)By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse and blackstrap
It is well mixed to obtain compound, then Mixed Microbes are added into compound, and add water and be well mixed, make the mixture moisture content be
50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are made up of bacillus subtilis and EM bacterium solutions, withered
The weight of careless bacillus and EM bacterium solutions ratio is 1:1.5;
(4)After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days;It is uniform to add lime powder and stirring, stands
2-3 days;Obtain compost;
(5)Compost is put into polybag, tightens sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature
Spend for 100-120 DEG C;
(6)After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
Preferably:Described punching loop-carrier sterilization is placed in sterilizing pan, and boiling in pot is opened into sterilization 15-30 minutes.
The preparation process of described punching loop-carrier is:
(1)Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars;
(2)Cylindrical bars are truncated into the cylindrical section of more 10-12 centimeter lengths;
(3)Tip is made in cylindrical section both ends;
(4)By be made the cylindrical section both sides after tip make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle strains
Groove;The width of strain groove is 3-5 millimeters, and depth is 2-3 millimeters, and the spacing distance of strain groove is 2-3 millimeters;
(5)The cylindrical section for making strain groove is cut off from centre, you can obtain two punching loop-carriers.
Preferably:The strain groove of punching loop-carrier both sides is respectively 4-5.
Ramulus mori, Classification system:Morus alba L, be the branches and leaves of mulberry tree, ramulus mori, mulberry shoot, RAMULUS MORI, general name.Fallen leaves
Shrub or dungarunga, high 3-15m.Bark canescence, there is strip is shallow to split;Root skin yellowish-brown or reddish yellow, fibroid are strong.Single leaf is mutual
It is raw;The long l-2.5cm of petiole;Blade is avette or width egg shape, and long 5-20cm, wide 4-10cm, tip is sharp sharp or tapering, basal circular or
Near heart-shaped, there are rough sawn tooth or knuckle-tooth in edge, there is irregular division sometimes, above it is hairless, it is glossy, below have undercoat on arteries and veins,
Hairiness between armpit, base go out 3, arteries and veins and reticulated with thready pulse intertexture, and the back side is more apparent;Stipule lanceolar, it is caducous.
Corn ear slag is remaining slag after corn rod threshing.
Bagasse is the accessory substance of cane sugar factory, is all as fuel or paper making raw material all the time, causes resource
It is a large amount of to waste.The present invention uses bagasse to prepare White mushroom planting material for raw material, belongs to the item of turning waste into wealth of leftover bits and pieces recycling
Mesh, waste residue is not discharged externally, it is green;Contain substantial amounts of cellulose, the bagasse after everfermentation, crude fibre in bagasse
Can degrade, crude protein can improve, and its nutritive value is improved, formed White mushroom bacterium germination and growth needed for albumen and
Nutrient.
Described bacillus subtilis, Classification system:Bacillussubtilis, be bacillus one kind.Withered grass
0.7~0.8 × 2~3 microns of bacillus individual cells, uniform coloring.Without pod membrane, peritrichous, can move.Gram-positive
Bacterium, 0.6~0.9 × 1.0~1.5 microns of gemma, ellipse arrive column, central or slightly inclined positioned at thalline, and thalline is not after sporulation
Expand.Bacterium colony rough surface is opaque, dirty white or slightly yellow, and when being grown in liquid medium within, wrinkle mould is commonly formed.Withered grass bud
Spore bacillus has stronger protease, amylase and lipase active, can be active enzyme by internal activation of zymogen, can also divide
- serial other enzymes are secreted, such as pectase, dextranase, cellulase, can help to decompose the thing such as SNSP in plant feed
Matter, the present invention decompose the thick of pine tree branch, pine wood sawdust, mulberry branch, corn ear slag and bagasse using bacillus subtilis
Fiber, form albumen and nutrient of the pleurotus eryngii bacterium germination needed for growth.
EM bacterium(Effective Microorganisms)It is into EM bacterium are big by Japanese Ryukyu by about 80 kinds of microorganism groups
Ratio is good to be studied successfully professor's nineteen eighty-two according to husband, is put goods on the market the eighties.EM bacterium are with photosynthetic bacteria, lactic acid bacteria, yeast
A kind of micro- life bacteria preparation that more than 80 microorganisms of 10 category based on bacterium and actinomyces are combined.The mechanism of action is to form EM
Bacterium and the competition of pathogenic microorganism contention nutrition, because EM bacterium easily live and reproduce in soil, so can it is very fast and stably
Occupy the ecologic niche in soil, form the advantage group of beneficial microbial bacteria, so as to control the breeding of pathogenic microorganism and
Invasion and attack to crop.It is the developing direction of the ecological agriculture, is more beneficial for the sustainable development of agricultural.The beginning of the nineties at the end of the eighties,
EM bacterium by the states such as Japan, Thailand, Brazil, the U.S., Indonesia, Sri Lanka be widely used in agricultural, cultivation, plantation,
The fields such as environmental protection, achieve obvious economic benefit and ecological benefits.
Above-mentioned strain is cultivated to obtain by Guangxi Academy Of Sciences.
The present invention substantive distinguishing features and marked improvement be:
1st, the inoculation method of this pleurotus eryngii culture medium is inserted in pleurotus eryngii quel strains using special punching loop-carrier, pleurotus eryngii quel strains
It is attached in the strain groove of punching loop-carrier, the punching loop-carrier with pleurotus eryngii quel strains is inserted not from the face of culture medium in linear
Enter culture medium, punching loop-carrier can not be taken out after insertion, allows it to stay in culture medium;Strain can be planted quickly in culture medium,
The contaminated probability of strain is reduced, and strain is combined closely with cultivating in a fungus bag base, the strain kind per cave enters amount equilibrium, plants into depth
Also it is balanced, reduce the irregular probability of fruiting;And inoculation time is that tradition first punches 3/5ths of the inoculation time being inoculated with afterwards.
2nd, golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into 2 by the application
The particle of the following size of millimeter;By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and sweet
Bagasse is well mixed to obtain compound with blackstrap, then Mixed Microbes are added into compound, and is added water and be well mixed, and makes mixing
Material water content is 50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are by bacillus subtilis and EM bacterium
Liquid forms, and the weight ratio of bacillus subtilis and EM bacterium solutions is 1:1.5;The compound for adding Mixed Microbes is placed on Indoor Natural hair
After ferment 5-7 days;It is uniform to add lime powder and stirring, stands 2-3 days;Obtain compost;Compost is put into polybag, tightened
Sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, and vapor (steam) temperature is 100-120 DEG C;After sterilization terminates, it will be equipped with
The polybag of compost takes out, and naturally cools to normal temperature, produces culture medium.Obtained culture medium is adapted to Growth of Pleurotus eryngii, ensures
Cultivating rate, and fruiting is fast, mycelia is non-aging.Culture medium was fermented using bacillus subtilis, EM strains and pulverized limestone, was improved
The disease-resistant and anti-miscellaneous bacteria infection ability of pleurotus eryngii.
Embodiment 1
The inoculation of pleurotus eryngii culture medium can be completed using following processing step:
(1)The compost of Xinbao mushroom culturing is loaded in polybag, sack is tightened, culture medium is obtained after sterilizing, it is standby;
(2)The multiple bamboo or wooden punching loop-carrier with multiple strain grooves is placed in sterilizing pan, boiling in pot is opened
15-30 minutes are sterilized, it is standby to take out natural cooling;
(3)By in the punching loop-carrier insertion pleurotus eryngii quel strains after sterilization, pleurotus eryngii quel strains are allowed to be attached to the strain of punching loop-carrier
In groove;
(4)Punching loop-carrier with pleurotus eryngii quel strains is submerged into culture medium from the face of culture medium in linear insert, after insertion not
Punching loop-carrier can be taken out, allows it to stay in culture medium;Distance every 10-15cm inserts a punching loop-carrier;
(5)The culture medium pile for punching loop-carrier will be plugged on culturing rack, insertion punches the culture medium of loop-carrier one side upward,
It can complete to be inoculated with.
The preparation process of described culture medium is:
(1)Golden peach ma branch 20-35 parts, mulberry branch 10-20 parts, anistree branch 10-15 parts, peanut shell 8-10 are taken by weight
Part, corn ear slag 5-8 parts, bagasse 3-5 parts, blackstrap 2-3 parts and pulverized limestone 1-1.5 parts;
(2)Golden peach ma branch, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse powder are broken into less than 2 millimeters
The particle of size;
(3)By the golden peach ma branch after crushing, mulberry branch, anistree branch, peanut shell, corn ear slag and bagasse and blackstrap
It is well mixed to obtain compound, then Mixed Microbes are added into compound, and add water and be well mixed, make the mixture moisture content be
50-65%;The weight of compound and Mixed Microbes ratio is 100:(2-3);Mixed Microbes are made up of bacillus subtilis and EM bacterium solutions, withered
The weight of careless bacillus and EM bacterium solutions ratio is 1:1.5;
(4)After the compound for adding Mixed Microbes is placed on into Indoor Natural fermentation 5-7 days;It is uniform to add lime powder and stirring, stands
2-3 days;Obtain compost;
(5)Compost is put into polybag, tightens sack;Pile is put into steam sterilizing pan, sterilizes 6-10 hours, steam temperature
Spend for 100-120 DEG C;
(6)After sterilization terminates, the polybag equipped with compost is taken out, normal temperature is naturally cooled to, produces culture medium.
The preparation process of described punching loop-carrier is:
(1)Bamboo material or timber are lathed to a diameter of 0.5-1 centimetres of cylindrical bars;
(2)Cylindrical bars are truncated into the cylindrical section 1 of more 10-12 centimeter lengths;
(3)Tip 2 is made in the both ends of cylindrical section 1;
(4)By be made the both sides of cylindrical section 1 after tip 2 make respectively it is multiple it is parallel with cylindrical section axis be in 35-50 degree angle bacterium
Kind groove 3;The width of strain groove 3 is 3-5 millimeters, and depth is 2-3 millimeters;
(5)The cylindrical section 1 for making strain groove 3 is cut off from centre, you can obtain two punching loop-carriers 4;Ensure punching inoculation
The strain groove of rod both sides is respectively 5.
Application Example
1st, Jinxiu Yao ethnic group in Guangxi province autonomous county Huang, original planting pleurotus eryngii in inoculation is first struck with a stick behind cave, artificial
Strain is filled in cave, a people can be inoculated with 22 bag culture mediums in one hour;The neat rate of fruiting is less than 75%;Later Huang's profit
With in the punching loop-carrier insertion pleurotus eryngii quel strains of the present invention, pleurotus eryngii quel strains are allowed to be attached in the strain groove of punching loop-carrier;Will
Punching loop-carrier with pleurotus eryngii quel strains submerges culture medium from the face of culture medium in linear insert, and punching can not be taken out after insertion
Loop-carrier, it is allowed to stay in culture medium;One people can be inoculated with more than 36 bags per hour;The neat rate of fruiting is more than 93%;Through statistics
Daily fruiting amount can receive 3 days mushrooms more than first beating behind cave 2.5% more than the fruiting amount being inoculated with.
Described above is not limitation of the present invention, and the present invention is also not limited to examples detailed above, the art it is general
Logical technical staff, in the essential scope of the present invention, the variations, modifications, additions or substitutions made, it should all belong to the guarantor of the present invention
Protect scope.