CN107714636A - Anti-aging skin care product containing fleece-flower root stem cell extract and preparation method thereof - Google Patents
Anti-aging skin care product containing fleece-flower root stem cell extract and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The invention discloses a kind of anti-aging skin care product, the skin care item contain fleece-flower root stem cell extract.Innovation uses fleece-flower root stem cell extract in anti-aging skin care product of the present invention, from cell repair aspect the skin care item can be made to have preferable senile-resistant efficacy；In addition, fleece-flower root stem cell extract can also further promote the further performance of other functional component effects in skin care item, the skin care item are further made to reach more preferable senile-resistant efficacy.Meanwhile the invention also discloses a kind of preparation method of the anti-aging skin care product.
Description
Technical field
It is especially a kind of anti-containing fleece-flower root stem cell extract the present invention relates to a kind of skin care item and preparation method thereof
Aging skin care item and preparation method thereof.
Background technology
Skin aging is a progressive physiology course, and the various factors comprehensive function such as heredity, environment, social mentality
Result.As the improvement of people's living standards, the functional requirement more and more higher to skin care item, possesses the skin care of anti-aging effects
Product also emerge in an endless stream.The approach of anti-aging, mainly includes：Protecting the skin from external environment stimulates, is in scavenger-cell unnecessary
Free radical, Skin Cell is repaired and supplemented the nutrients.Skin anti-aging is to prevent skin from occurring gradually because the time elapses
Property function sexually revise, show as preventing wrinkle, drying, play bits, relaxation and color spot.Anti-aging cosmetics are exactly to realize to resist
One of the effect of cosmetics of aging effect are important type cosmetics.
The current Yi Zhan cosmetics sales main market players of anti-aging cosmetics, most of product are all main from skin senescence
Factor：Photo-aging and free radical are started with, and add such as superoxide dismutase (SOD), tocopherol acetate, Vitamin C
Acid, retinol, free radical is hindered to form peroxide, effect is relatively simple, while there is also stability is poor, easy to change and allergy
The problems such as.
Present anti-aging cosmetics some has used the strong industry of inoxidizability to reach good anti-aging effects
It is composition, quick in short-term, but it is very big using harm to the human body for a long time；Some anti-aging cosmetics add vitamins antioxygen
Agent, but this kind of antioxidant stability is poor.By the natural plant extracts for having senile-resistant efficacy be made stability it is good into
Divide and be added in cosmetics, assign product good senile-resistant efficacy, be a trend of anti-aging product.
The content of the invention
Based on this, a kind of antisenility skin care is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
Product.
To achieve the above object, the technical solution used in the present invention is：A kind of anti-aging skin care product, the skin care item contain
There is fleece-flower root stem cell extract.
Contain fleece-flower root stem cell extract in skin care item of the present invention, there is preferable senile-resistant efficacy, this be because
Dormancy fibroblast can be quickly activated for fleece-flower root stem cell extract, promotes cell division, propagation, lifts cell DNA
Injury repair ability, promote cell self-regeneration renewal, secrete Collagen material, adjust collagenous fibres, strengthen skin elasticity, have
Strong anti-oxidation, free radical is removed, alleviate pigment color spot calmness, while assign skin adequate water and SuperPro outlet capacity, allowed
Skin high resilience, compact it is smooth.
In addition, fleece-flower root stem cell extract can also further promote the further of other functional component effects in skin care item
Play, the skin care item is reached preferable senile-resistant efficacy.
Preferably, weight/mass percentage composition of the fleece-flower root stem cell extract in the skin care item be 0.5~
2.8%.Present inventor has found that the fleece-flower root stem cell extract of above-mentioned content can preferably play work in skin care item
With more preferable senile-resistant efficacy can be played preferably with the other compositions collective effect in skin care item.
It is highly preferred that the skin care item also include sweet potato extract, matter of the sweet potato extract in the skin care item
It is 5~10% to measure percentage composition.
Sweet potato extract：Sweet potato is rich in sugar, vitamin A, vitamin C etc., nutritious, contains albumen in every 100 grams of sweet potato
1.8 grams, 9 kinds 303 milligrams of amino acid of matter, 22 grams of carbohydrate, 31.97 milligrams of multivitamin；Particularly Vitamin C content exceedes apple
Fruit, pears, apricot, peach, several times to more than 30 times of grape contained amount, also contain a kind of material of similar female hormone in sweet potato, to protecting
Human body skin is protected, anti-aging has certain effect.Therefore, external many women sweet potato as conditioning skin and losing food.
Fleece-flower root stem cell extract in skin care item of the present invention, it can further promote cell to work(in sweet potato extract
The absorption of composition is imitated, further plays more preferable senile-resistant efficacy.
It is highly preferred that the skin care item also include butter berry extract, the butter berry extract is in the skin care item
Weight/mass percentage composition be 3~8%.
Butter berry extract：It contains abundant glyceric acid, protein and vitamin, profit and it is oiliness, be natural antioxygen
Aging agent, it can not only soften and skin care, moreover it is possible to receives fine, soft fur hole, skin surface can form emulsus separation layer, Neng Gouyou
Effect resists sunlight, prevents tanned sunburn.Galam butter is nutritious in butter berry extract, containing abundant vitamin E,
Magnesium, linoleic acid and essential fatty acid, contribute to tough cell membrane, delay the speed of epidermal cell aging.
Fleece-flower root stem cell extract in skin care item of the present invention, it can further promote cell in butter berry extract
The absorption of functional component, further play more preferable senile-resistant efficacy.
It is highly preferred that the skin care item also include white willow bark extract, the white willow bark extract is in the skin care
Weight/mass percentage composition in product is 8~14%.
White willow bark extract：White willow bark extract plays the role of widely to suppress human chitinase, in low concentration to three
Kind metalloproteinases has inhibitory action, and activation and the skin aging of metalloproteinases have direct relation, plus white willow bark
Strong elimination of the extract to superoxide radical, therefore white willow bark extract can be used for the anti-ageing composition of cosmetics, to black
Plain Carbazole alkaloid effect is fine, is better than ursin.
Fleece-flower root stem cell extract in skin care item of the present invention, can further promote cell dialogue extract of willow bark
The absorption of middle functional component, further play more preferable senile-resistant efficacy.
It is highly preferred that the fleece-flower root stem cell extract, sweet potato extract, butter berry extract, white willow bark extract
The weight ratio of thing is：Fleece-flower root stem cell extract：Sweet potato extract：Butter berry extract：White willow bark extract=(1~
2.2)：7：5:11.Above-mentioned weight than four kinds of compositions, can preferably play synergistic function, reach more preferable anti-aging
Effect.
It is highly preferred that the fleece-flower root stem cell extract, sweet potato extract, butter berry extract, white willow bark extract
The weight ratio of thing is：Fleece-flower root stem cell extract：Sweet potato extract：Butter berry extract：White willow bark extract=1.5：
7：5:11.Above-mentioned weight than four kinds of compositions, performance synergistic function that can be best reaches more preferable anti-aging effects.
In addition, it is noted that skin care item of the present invention can also include auxiliary material, the selection of auxiliary material has no tool herein
Body limits, and those skilled in the art can carry out conventional selection such as water, alcohols, finish, surfactant, pH according to being actually needed
The auxiliary materials such as conditioning agent, antioxidant or preservative.In addition, skin care item of the present invention can be made creme, emulsion, essence lotion,
The diversified forms such as gel or facial mask, the present invention have no specific restriction to this, and those skilled in the art can also be according to actual need
Carry out conventional selection.
Meanwhile invention additionally discloses a kind of preparation method of above-mentioned anti-aging skin care product, methods described to be：Will what head
Black stem cell extract, sweet potato extract, butter berry extract, the mixing of white willow bark extract, add deionized water and eat
Alcohol, after agitated, centrifugation, filtering, regulation PH to faintly acid, sterilizing, homogeneous is carried out with the other compositions in the skin care item,
Produce anti-aging skin care product of the present invention.
Preferably, the fleece-flower root stem cell is prepared by the following method gained：
(1) fleece-flower root forming layer stem cell is separated and cultivated；
(2) then the fleece-flower root forming layer stem cell after being cultivated in step (1) is concentrated into filtrate first through ultrafiltration membrance filter,
Remove phenol red in culture medium, the active component in reservation culture medium；Finally by culture medium and fleece-flower root forming layer stem cell
Lysate is freeze-dried, and obtains the fleece-flower root stem cell extract.
It is highly preferred that the separation of fleece-flower root forming layer stem cell, incubation are in the step (1)：By the fleece-flower root
Root tuber cuts the semicircle section including forming layer after disinfecting；Semi-circular section is placed on WPM culture mediums
On, dark culturing at 25 DEG C；The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and shifts
Cultivated into subculture medium, subculture once, obtains fleece-flower root forming layer stem cell every two weeks.
Relative to prior art, beneficial effects of the present invention are：
Innovation uses fleece-flower root stem cell extract in anti-aging skin care product of the present invention, has preferable anti-aging work(
Effect, because fleece-flower root stem cell extract can quickly activate dormancy fibroblast, promote cell division, propagation, carry
DNA Damage repair ability is risen, promotes cell self-regeneration renewal, secretes Collagen material, adjust collagenous fibres, strengthen skin
Elasticity, there is strong anti-oxidation, remove free radical, alleviate pigment color spot calmness, while assign skin adequate water and SuperPro
Outlet capacity, allow skin high resilience, compact it is smooth.
In addition, fleece-flower root stem cell extract can also further promote the further of other functional component effects in skin care item
Play, the skin care item is reached preferable senile-resistant efficacy.
And skin care item formula of the present invention is simply, raw material almost all derives from plant component, not only avoiding
Learn the stimulation of synthetic ingredient, and can also from the root of cell metabolism, constantly replace senile cell, promote cell self to repair
It is multiple, it is long-term use of, extraordinary anti-aging effects can be reached.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.Skin care item described in the embodiment of the present invention can also include auxiliary material, and auxiliary material selection has no specific restriction herein, this
Art personnel can carry out conventional selection such as water, alcohols, finish, surfactant, pH adjusting agent, anti-according to being actually needed
The auxiliary material such as oxidant or preservative, in addition, the skin care item in embodiment are analyzed its performance only by taking toner as an example, but not
Limitation and this, other such as the compliance test result of the skin care item of creme, emulsion, essence lotion, gel or facial mask diversified forms and this
It is similar.
7 kinds of embodiments of anti-aging skin care product of the present invention, it is by percentage to the quality, anti-ageing described in embodiment 1~7
The composition and content of old skin care item are as shown in table 1, and set control group, and fleece-flower root stem cell extract is not contained in control group
Outside, other compositions are same as Example 1：
Composition and content in the embodiment 1~7 of table 1 in anti-aging skin care product
Skin care item are made in composition in above-described embodiment 1~7 and control group, the skin care item are toner, described refreshing
Skin water also includes the composition of following weight/mass percentage composition：Propane diols 3%, butanediol 2%, hydrolyze Jojoba esters 0.4%, be transparent
Matter acid sodium 0.03%, essence and preservative, appropriate amount of water.
The preparation method of the skin care item is：By the fleece-flower root stem cell extract, sweet potato extract, butter fruit extraction
Thing, the mixing of white willow bark extract, add deionized water and edible alcohol, agitated, centrifugation, filtering, regulation PH to faintly acid,
After sterilizing, homogeneous is carried out with the other compositions in the skin care item, obtains the anti-aging skin care product.
The fleece-flower root stem cell is prepared by the following method gained：
(1) fleece-flower root forming layer stem cell is separated and cultivated：By the root tuber of the fleece-flower root after disinfecting, cut
Semicircle section including forming layer down；Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；Two weeks
The outer paraphyte of forming layer had significant proliferation is taken out afterwards, forming layer stem cell is separated and is transferred in subculture medium and cultivate, often
Two weeks subcultures once, obtain fleece-flower root forming layer stem cell.
(2) then the fleece-flower root forming layer stem cell after being cultivated in step (1) is concentrated into filtrate first through ultrafiltration membrance filter,
Remove phenol red in culture medium, the active component in reservation culture medium；Finally by culture medium and fleece-flower root forming layer stem cell
Lysate is freeze-dried, and obtains the fleece-flower root stem cell extract.
Embodiment 8
Strengthen skin elasticity compliance test result
Subject Population is divided into 8 groups, every group of 20 people, the tested objective packet of crowd, and it is ignorant to product composition, exclude
Influence of the psychological factor to the product overall evaluation.Each group of people uses the skin-protection product of embodiment 1~7 respectively.Before sample is smeared, by
Examination person is using the gentle cleaning agent cleaning face of identical, and symmetric position selectes about 5 × 5cm at left and right sides of subject face2Greatly
The skin-protection product that about 0.5g each groups accordingly use is smeared, frequency of use is once in the morning and once at night, even as Experimental Area in small region
It is continuous to use daily.
Using before the skin-protection product of embodiment 1~7 and control group, in every subject's test zone Callegari
The each experiment position initial elasticity m of spring probe detection of soft plus skinanalysis apparatus0Value, is repeated 5 times, draws flat respectively
Average.Detected respectively again at these positions after using 2,4,6 weeks, each test zone respectively 5 hypoelasticity m values of detection, and asking
Go out 5 m of period average value.
In order to preferably evaluate the data obtained, the m detected every time respectively divided by is not used the m of sample0Averagely it is worth to
The elasticity grading at the position, when elastic grading value is bigger, illustrate that sample increase skin elasticity is more obvious.Use embodiment 1~7
And the elasticity grading of control set product is as shown in table 2.
The elasticity grading of the embodiment 1~7 of table 2 and control set product
From table 2 it can be seen that compared with control group, after 6 weeks, using the skin care item in the embodiment of the present invention 1~7,
Higher elasticity grading is respectively provided with, the anti-aging effects of the skin care item in this explanation embodiment 1~7 are fine, also furtherly
Performance of the bright fleece-flower root stem cell extract of the present invention to other compositions effect in skin care item serves facilitation, wherein making
With the elasticity grading highest of the skin care item in embodiment 1, this illustrates that the anti-aging effects of the skin care item in embodiment 1 are best；Together
When, after 6 weeks, the elasticity grading of skin care item is higher than the elasticity grading of the skin care item of embodiment 2 and 3, embodiment 1 in embodiment 1
~3 are above the elasticity grading of skin care item in embodiment 4 and 5, and the elastic of skin care item is graded in embodiment 1~3, embodiment 6~7
Higher than the elasticity grading of the skin care item of embodiment 4 and 5, this is also why the application selects fleece-flower root stem cell extract described
Weight/mass percentage composition in skin care item is 0.5~2.8%, fleece-flower root stem cell extract：Sweet potato extract：Butter fruit is extracted
Thing：White willow bark extract=(1~2.2)：7：5:11, and further preferably fleece-flower root stem cell extract：Sweet potato extract：
Butter berry extract：White willow bark extract=1.5：7：5:11 the reason for.
Embodiment 9
Experiment is divided into control group and experimental group, and experimental group 1~7 is respectively prepared skin care item in embodiment 1~7, is compareed
Component is 14 groups, and contained component is except fleece-flower root stem cell extract, sweet potato extract, ox in the skin care item of control group 1~14
Oily berry extract, the ratio of four kinds of compositions of white willow bark extract are different outer, and fleece-flower root stem cell extract, sweet potato extraction
The total amount of thing, four kinds of butter berry extract, white willow bark extract compositions in skin care item is identical, and other compositions are and embodiment
1 is identical, and fleece-flower root stem cell extract, sweet potato extract, butter berry extract, white willow bark carry in specific control group 1~14
Take the ratio of four kinds of compositions of thing as shown in table 3：
Fleece-flower root stem cell extract, sweet potato extract, butter berry extract, white willow bark in the control group skin care item of table 3
The proportional amount of four kinds of compositions of extract
In control group and experimental group, choose 100 ages for every group and carry out research on probation in the volunteer of 20~50 years old, it is real
Test the skin care item that the subjects of group 1~7 are prepared using embodiment 1~7 respectively, the subjects point of control group 1~14
The skin care item not being prepared using control group 1~14, skin care item application method is as in embodiment 8, if after 6 weeks, surveying
Obtain elastic grading and be higher than 1.65, be considered as effectively, statistics effective percentage, as a result as shown in table 4.
The effective situation statistical result of elasticity grading in the experimental group of table 4 and control group
As seen from Table 4, in the case that total amount in four kinds of compositions in skin care item is certain, the skin care of control group 1~14
As long as containing fleece-flower root stem cell extract in product, all with certain senile-resistant efficacy, when fleece-flower root stem cell extract and
Sweet potato extract, butter berry extract, one kind in white willow bark extract, two kinds, three kinds with the use of when, senile-resistant efficacy
Gradually increase, this illustrates that the performance that fleece-flower root stem cell extract of the present invention is acted on other compositions is played and promotes to make well
With；Moreover, when fleece-flower root stem cell extract and three kinds of sweet potato extract, butter berry extract, white willow bark extract cooperations
In use, senile-resistant efficacy will be apparently higher than fleece-flower root stem cell extract and sweet potato extract, butter berry extract, white willow
One kind in bark extract, two kinds with the use of when effect, this explanation fleece-flower root stem cell extract and sweet potato extract, ox
When oily berry extract, white willow bark extract are used cooperatively, the effect of Synergistic is served.
Generally speaking, sweet potato extract, butter berry extract, white willow bark are comprised only in the skin care item of control group 1~14
One kind in four kinds of compositions of extract, two or three, without good anti-aging effects.And the skin care item of experimental group 1~7
In contain four kinds of compositions, there are good anti-aging effects.Thus it is provable, in skin care item of the invention, fleece-flower root stem cell
Extract, sweet potato extract, butter berry extract, white willow bark extract four kinds of compositions, which have, mutually promotes, mutually cooperates with
Effect, can play significant anti-aging effects.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of anti-aging skin care product, it is characterised in that the skin care item contain fleece-flower root stem cell extract.
2. anti-aging skin care product as claimed in claim 1, it is characterised in that the fleece-flower root stem cell extract is in the shield
Weight/mass percentage composition in skin product is 0.5~2.8%.
3. anti-aging skin care product as claimed in claim 1 or 2, it is characterised in that also carried comprising sweet potato extract, the sweet potato
It is 5~10% to take weight/mass percentage composition of the thing in the skin care item.
4. anti-aging skin care product as claimed in claim 3, it is characterised in that also comprising butter berry extract, the butter fruit
Weight/mass percentage composition of the extract in the skin care item is 3~8%.
5. anti-aging skin care product as claimed in claim 4, it is characterised in that also comprising white willow bark extract, the white willow
Weight/mass percentage composition of the bark extract in the skin care item is 8~14%.
6. anti-aging skin care product as claimed in claim 5, it is characterised in that the fleece-flower root stem cell extract, sweet potato carry
The weight ratio for taking thing, butter berry extract, white willow bark extract is：Fleece-flower root stem cell extract：Sweet potato extract：Butter
Berry extract：White willow bark extract=(1~2.2)：7：5:11.
7. anti-aging skin care product as claimed in claim 6, it is characterised in that the fleece-flower root stem cell extract, sweet potato carry
The weight ratio for taking thing, butter berry extract, white willow bark extract is：Fleece-flower root stem cell extract：Sweet potato extract：Butter
Berry extract：White willow bark extract=1.5：7：5:11.
A kind of 8. preparation method of the anti-aging skin care product as described in any one of claim 1~7, it is characterised in that methods described
For：The fleece-flower root stem cell extract, sweet potato extract, butter berry extract, white willow bark extract are mixed, addition is gone
Ionized water and edible alcohol, after agitated, centrifugation, filtering, regulation PH to faintly acid, sterilizing, with other in the skin care item
Composition carries out homogeneous, produces anti-aging skin care product of the present invention.
9. the preparation method of anti-aging skin care product as claimed in claim 8, it is characterised in that the fleece-flower root stem cell passes through
Following methods prepare gained：
(1) fleece-flower root forming layer stem cell is separated and cultivated；
(2) then the fleece-flower root forming layer stem cell after being cultivated in step (1) is concentrated into filtrate first through ultrafiltration membrance filter, removed
It is phenol red in culture medium, retain the active component in culture medium；Finally by the cracking of culture medium and fleece-flower root forming layer stem cell
Thing is freeze-dried, and obtains the fleece-flower root stem cell extract.
10. the preparation method of anti-aging skin care product as claimed in claim 9, it is characterised in that the fleece-flower root in the step (1)
The separation of forming layer stem cell, incubation are：By the root tuber of the fleece-flower root after disinfecting, cut including forming layer
Semicircle section；Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；Forming layer is substantially increased after two weeks
The outer paraphyte grown takes out, and separates forming layer stem cell and is transferred in subculture medium and cultivate, subculture once, obtains every two weeks
Fleece-flower root forming layer stem cell.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110833520A (en) * | 2019-12-14 | 2020-02-25 | 广州伊尔美生物科技有限公司 | Anti-aging composition |
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| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110833520A (en) * | 2019-12-14 | 2020-02-25 | 广州伊尔美生物科技有限公司 | Anti-aging composition |
| CN110833520B (en) * | 2019-12-14 | 2022-02-01 | 广州伊尔美生物科技有限公司 | Anti-aging composition |
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