CN107667923A - A kind of hybridized prussian carp large-scale artificial reproduction technique - Google Patents
A kind of hybridized prussian carp large-scale artificial reproduction technique Download PDFInfo
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- CN107667923A CN107667923A CN201711056416.5A CN201711056416A CN107667923A CN 107667923 A CN107667923 A CN 107667923A CN 201711056416 A CN201711056416 A CN 201711056416A CN 107667923 A CN107667923 A CN 107667923A
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- carp
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of hybridized prussian carp large-scale artificial reproduction technique, comprise the following steps：(1) using Singuo Red Carp as male parent, breeding method is consistent with common carp；Using upright crucian carp as female parent, female parent is cultivated with male parent point pond, carries out bait throwing in training, controls feeding rate, changes water, oxygenation, 15d stops changing water before breeding period is closed on；(2) ocyodinic is made into parenteral solution and once injects fish body；(3) fish nest is made along root and palm bark with willow, fish nest is arranged in underwater at spawning pond offshore immediately after step (2) is hastened parturition；(4) preparation of spawning pond；(5) using hydrostatic inflation hatching method hatching.Hybridized prussian carp large-scale artificial reproduction technique of the present invention; enable hybridized prussian carp breed out high quality Crucian Carp Fry and its fry emergence rate and survival rate it is all high; ensure the production requirement of Crucian Carp Fry and improve the economic benefit at hybridized prussian carp breeding family, improve integrated ecological benefit and effect, economic benefit and the social benefit of sustainable development and the foster fishery of hybridized prussian carp aquaculture.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of hybridized prussian carp large-scale artificial reproduction technique.
Background technology
Hybridized prussian carp is the Gynogenetic Offspring of upright pond crucian carp ovum and Singuo Red Carp sperm fertilization.It was cultivated from 1976
Cultivation has been promoted in the whole nation since success, achieves preferable economic results in society.Hybridized prussian carp meat flavour is delicious, nutritious,
By the favor of the masses.In order to which further genralrlization cultivates, the supply of high-quality seed must meet as far as possible.
At present, a kind of excellent product that hybridized prussian carp system excites pond crucian carp ovum to carry out gynogenesis and bred using Heterologous Sperm
Kind, this breeding technique has been applied to production practices at present.Though hybridized prussian carp artificial propagation techniques are uncomplicated, small lot
Breeding in general plant can accomplish, but to carry out large-scale artificial breeding just must be ripe to each link of reproduction technique
Know, slightly mishap, it is possible to cause larger economic loss to production.
The content of the invention
To solve above-mentioned the shortcomings of the prior art, the present invention provides a kind of hybridized prussian carp large-scale artificial breeding skill
Art, by the technology enable hybridized prussian carp breed out high quality Crucian Carp Fry and its fry emergence rate and survival rate all
Height, it so can ensure that the production requirement of Crucian Carp Fry and be greatly enhanced the economic benefit at hybridized prussian carp breeding family, can pole
The earth improves integrated ecological benefit and effect, economic benefit and the social benefit of sustainable development and the foster fishery of hybridized prussian carp aquaculture.
To achieve these goals, the present invention provides following technical scheme, and a kind of hybridized prussian carp large-scale artificial breeds skill
Art, comprise the following steps：
（1）Parent fish rearing：Thoroughly clean up the pond before parent population is put in a suitable place to breed, first filter during water inlet, and the weeds of cleared culture pond surrounding；With emerging
The red carp of state is male parent, 2 ~ 3 ages, 500 ~ 1000g of body weight, and breeding density is 2500-3500kg/ squares of hectare, breeding method with it is general
Logical carp is consistent；Using upright crucian carp as female parent, in age at age 2 ~ 3, more than body weight 250g, breeding density is 2000-3000kg/ squares of public affairs
Hectare, female parent is cultivated with male parent point pond, when spring water temperature rises to 10 DEG C, upright crucian carp is called in greenhouse progress bait throwing in training, after 5d
Every morning and each bait throwing in afternoon l times, feeding rate control change water l times, every night is increased with inflator pump in 0.5%-l% per 7-10d
Oxygen, dissolved oxygen amount are maintained at more than 5mg/L；Day in autumn feeding amount is the 3%-5% of fish body gross weight；After entering the winter, day feeding amount is fish
The 1%-2% of body gross weight；After the beginning of spring, day feeding amount is fed by the 3% ~ 5% of fish body gross weight, and 15d stops before breeding period is closed on
Change water；
（2）Hasten parturition：Ocyodinic is injected into 2mL by every kilogram of fish, is made into parenteral solution with 0.7% physiological saline, injection site is in parent
Fish pectoral fin base portion, injection needle are pierced into fish body axle angle at 45 °, and ocyodinic is once injected into fish body；
（3）The making arrangement of fish nest：Fish nest is made along root and palm bark with willow, is soaked using preceding with 3% limewash
15min or boiling 30min, is then bundled into tie in from base portion and is tied to standby on bamboo pole, and bamboo pole length is 1.5m, per beam fish nest interval
20-30cm；In step（2）Fish nest is arranged at spawning pond offshore at the 10-15cm of underwater immediately after hastening parturition；
（4）The preparation of spawning pond：Spawning pond 8-10d before Gunther parent fish spawning is cleaned up the pond sterilization with quick lime, dosage 900-1200kg/
Square hectare, while remove debris in pond, the new water of 2-3d injections cleaning after cleaning up the pond, it is with close net filtration, the depth of water during water filling
1.0-1.2m；
（5）Artificial incubation：Hatching method is inflated using hydrostatic, water temperature is controlled after 21-22 DEG C, prelarva whole membrane, is aerated
Take a breath and Sunshade film is put on plastic film, prevent water temperature height, water quality overfertilization from prelarva is obtained bubble illness, prelarva is trained in hatchery
Educate to go out pond after 5-10d and be transferred to outdoor cultivation pond and continue to cultivate.
Further, the step（1）Culture pond depth of water 1.5-2m, bottom of pond is flat, non-leakage.
Further, for the water selection of time of changing at fine day noon, quantity of exchanged water accounts for the 1/3 of former water.
Further, the injected volume of the fish nest is per the beam of tail raun 1.
Further, during the ocyodinic injection, when natural spawning fertilization injection time is afternoon 13 ~ 15, egg-laying time
In morning next day to early morning；When artificial insemination injection time is afternoon 17 ~ 18, egg-laying time is at the next morning 6 ~ 8.
Further, the ocyodinic is luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and fish brain
One or more in hypophysis.
Further, the dosage of hastening parturition of the female pond crucian carp is：Fish hypophysis dosage is that every kilogram of fish re-injection penetrates 5 ~ 6
Mg, male carp dosage halve；Or every kilogram of fish injection μ g of luteotropin releasing hormone d-ala analog 30 and chorionic gonadotrophin
Hormone 1200IU, male carp dosage halve.
Further, during the ocyodinic injection, first injected to maternal, give male parent to inject again after 2-3h.
In summary, the present invention has the advantages that as a result of such scheme：
1st, the technology of the present invention Founder crucian carp is cultivated in greenhouse, can shift to an earlier date sexal maturity in 10-20 days, and Singuo Red Carp is outside greenhouse
Cultivate, synchronous maturation is can reach with the upright crucian carp of warm indoor culture, smoothly hastened parturition for it and provide guarantee.
2nd, when injecting ocyodinic, upright crucian carp does sth. in advance 2-3 hours and injected the present invention, and Singuo Red Carp synchronization of estrus, effectively
The spawning that have stimulated upright crucian carp, its egg laying amount ratio inject simultaneously ocyodinic improve more than 30%.
3rd, the present invention builds the natural propagation environment of hybridized prussian carp using pond, promotes rate of fertilization to improve, can typically reach
To more than 97%, reproductive efficiency is effectively improved, the problem for solving hybridized prussian carp seed propagation in scale.
Embodiment
With reference to embodiment, the present invention is described further.
A kind of hybridized prussian carp large-scale artificial reproduction technique, comprises the following steps：
（1）Parent fish rearing：Thoroughly clean up the pond before parent population is put in a suitable place to breed, first filter during water inlet, and the weeds of cleared culture pond surrounding；With emerging
The red carp of state is male parent, 2 ~ 3 ages, 500 ~ 1000g of body weight, and breeding density is 2500-3500kg/ squares of hectare, breeding method with it is general
Logical carp is consistent；Using upright crucian carp as female parent, in age at age 2 ~ 3, more than body weight 250g, breeding density is 2000-3000kg/ squares of public affairs
Hectare, female parent is cultivated with male parent point pond, when spring water temperature rises to 10 DEG C, upright crucian carp is called in greenhouse progress bait throwing in training, after 5d
Every morning and each bait throwing in afternoon l times, feeding rate control change water l times, every night is increased with inflator pump in 0.5%-l% per 7-10d
Oxygen, dissolved oxygen amount are maintained at more than 5mg/L；Day in autumn feeding amount is the 3%-5% of fish body gross weight；After entering the winter, day feeding amount is fish
The 1%-2% of body gross weight；After the beginning of spring, day feeding amount is fed by the 3% ~ 5% of fish body gross weight, and 15d stops before breeding period is closed on
Change water；
（2）Hasten parturition：Ocyodinic is injected into 2mL by every kilogram of fish, is made into parenteral solution with 0.7% physiological saline, injection site is in parent
Fish pectoral fin base portion, injection needle are pierced into fish body axle angle at 45 °, and ocyodinic is once injected into fish body；
（3）The making arrangement of fish nest：Fish nest is made along root and palm bark with willow, is soaked using preceding with 3% limewash
15min or boiling 30min, is then bundled into tie in from base portion and is tied to standby on bamboo pole, and bamboo pole length is 1.5m, per beam fish nest interval
20-30cm；In step（2）Fish nest is arranged at spawning pond offshore at the 10-15cm of underwater immediately after hastening parturition；
（4）The preparation of spawning pond：Spawning pond 8-10d before Gunther parent fish spawning is cleaned up the pond sterilization with quick lime, dosage 900-1200kg/
Square hectare, while remove debris in pond, the new water of 2-3d injections cleaning after cleaning up the pond, it is with close net filtration, the depth of water during water filling
1.0-1.2m；
（5）Artificial incubation：Hatching method is inflated using hydrostatic, water temperature is controlled after 21-22 DEG C, prelarva whole membrane, is aerated
Take a breath and Sunshade film is put on plastic film, prevent water temperature height, water quality overfertilization from prelarva is obtained bubble illness, prelarva is trained in hatchery
Educate to go out pond after 5-10d and be transferred to outdoor cultivation pond and continue to cultivate.
Wherein, step（1）Culture pond depth of water 1.5-2m, bottom of pond is flat, non-leakage；
Water selection of time is changed at fine day noon, quantity of exchanged water accounts for the 1/3 of former water；
The injected volume of fish nest is per the beam of tail raun 1；
When ocyodinic is injected, when natural spawning fertilization injection time is afternoon 13 ~ 15, egg-laying time is in morning next day to early morning；
When artificial insemination injection time is afternoon 17 ~ 18, egg-laying time is at the next morning 6 ~ 8；
Ocyodinic is one kind or several in luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and fish hypophysis
Kind；
The dosage of hastening parturition of female pond crucian carp is：Fish hypophysis dosage is that every kilogram of fish re-injection penetrates 5 ~ 6 mg, and male carp dosage subtracts
Half；Or every kilogram of fish injection μ g of luteotropin releasing hormone d-ala analog 30 and human chorionic gonadtropin 1200IU, male carp
Fish dosage halves；
When ocyodinic is injected, first injected to maternal, give male parent to inject again after 2-3h.
Using this hybridized prussian carp large-scale artificial reproduction technique, egg laying amount ratio injects ocyodinic and improves more than 30 % simultaneously, by
Smart rate reaches more than 97%, breeds in year more than the tail of hybridized prussian carp seedling 21,000,000.
The preferred embodiment of invention is the foregoing is only, is not intended to limit the invention, all spirit in the present invention
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (8)
1. a kind of hybridized prussian carp large-scale artificial reproduction technique, it is characterised in that the reproduction technique comprises the following steps：
（1）Parent fish rearing：Thoroughly clean up the pond before parent population is put in a suitable place to breed, first filter during water inlet, and the weeds of cleared culture pond surrounding；With emerging
The red carp of state is male parent, 2 ~ 3 ages, 500 ~ 1000g of body weight, and breeding density is 2500-3500kg/ squares of hectare, breeding method with it is general
Logical carp is consistent；Using upright crucian carp as female parent, in age at age 2 ~ 3, more than body weight 250g, breeding density is 2000-3000kg/ squares of public affairs
Hectare, female parent is cultivated with male parent point pond, when spring water temperature rises to 10 DEG C, upright crucian carp is called in greenhouse progress bait throwing in training, after 5d
Every morning and each bait throwing in afternoon l times, feeding rate control change water l times, every night is increased with inflator pump in 0.5%-l% per 7-10d
Oxygen, dissolved oxygen amount are maintained at more than 5mg/L；Day in autumn feeding amount is the 3%-5% of fish body gross weight；After entering the winter, day feeding amount is fish
The 1%-2% of body gross weight；After the beginning of spring, day feeding amount is fed by the 3% ~ 5% of fish body gross weight, and 15d stops before breeding period is closed on
Change water；
（2）Hasten parturition：Ocyodinic is injected into 2mL by every kilogram of fish, is made into parenteral solution with 0.7% physiological saline, injection site is in parent
Fish pectoral fin base portion, injection needle are pierced into fish body axle angle at 45 °, and ocyodinic is once injected into fish body；
（3）The making arrangement of fish nest：Fish nest is made along root and palm bark with willow, is soaked using preceding with 3% limewash
15min or boiling 30min, is then bundled into tie in from base portion and is tied to standby on bamboo pole, and bamboo pole length is 1.5m, per beam fish nest interval
20-30cm；In step（2）Fish nest is arranged at spawning pond offshore at the 10-15cm of underwater immediately after hastening parturition；
（4）The preparation of spawning pond：Spawning pond 8-10d before Gunther parent fish spawning is cleaned up the pond sterilization with quick lime, dosage 900-1200kg/
Square hectare, while remove debris in pond, the new water of 2-3d injections cleaning after cleaning up the pond, it is with close net filtration, the depth of water during water filling
1.0-1.2m；
（5）Artificial incubation：Hatching method is inflated using hydrostatic, water temperature is controlled after 21-22 DEG C, prelarva whole membrane, is aerated
Take a breath and simultaneously put Sunshade film on plastic film, prelarva is cultivated to go out pond after 5-10d and be transferred to outdoor cultivation pond in hatchery to continue to train
Educate.
A kind of 2. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：The step
（1）Culture pond depth of water 1.5-2m, bottom of pond is flat, non-leakage.
A kind of 3. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：It is described when changing water
Between selection at fine day noon, quantity of exchanged water accounts for the 1/3 of former water.
A kind of 4. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：The fish nest
Injected volume is per the beam of tail raun 1.
A kind of 5. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：The ocyodinic
During injection, when natural spawning fertilization injection time is afternoon 13 ~ 15, egg-laying time is in morning next day to early morning；Artificial insemination is noted
When to penetrate the time be afternoon 17 ~ 18, egg-laying time is at the next morning 6 ~ 8.
A kind of 6. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：The ocyodinic
For the one or more in luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and fish hypophysis.
A kind of 7. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that the female silver
The dosage of hastening parturition of crucian carp is：Fish hypophysis dosage is that every kilogram of fish re-injection penetrates 5 ~ 6 mg, and male carp dosage halves；Or every thousand
Gram fish injection μ g of luteotropin releasing hormone d-ala analog 30 and human chorionic gonadtropin 1200IU, male carp dosage halve.
A kind of 8. hybridized prussian carp large-scale artificial reproduction technique according to claim 1, it is characterised in that：The ocyodinic
During injection, first injected to maternal, give male parent to inject again after 2-3h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169426A (en) * | 2018-10-22 | 2019-01-11 | 广州市建波鱼苗场有限公司 | A kind of anti-season breeding method of crucian |
| CN111109160A (en) * | 2020-01-13 | 2020-05-08 | 广西壮族自治区水产引育种中心 | Method for breeding excellent variety of carp by hybridization |
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| CN101796932A (en) * | 2010-04-07 | 2010-08-11 | 江苏海辰科技集团 | Method for optimal seedling selection and breeding of novel carassius auratus gibelio series |
| CN101919362A (en) * | 2010-08-31 | 2010-12-22 | 中国水产科学研究院淡水渔业研究中心 | Semi-artificial breeding technology for Carassius aurutus gibelio |
| CN103563799A (en) * | 2012-08-03 | 2014-02-12 | 潘洪强 | Method for breeding allogynogenetic crucian carp fry at low temperature |
| CN104273069A (en) * | 2014-10-20 | 2015-01-14 | 苏州苏湘特种水产养殖场 | Nuisanceless allogynogenetic crucian crap breeding method |
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2017
- 2017-11-01 CN CN201711056416.5A patent/CN107667923A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101796932A (en) * | 2010-04-07 | 2010-08-11 | 江苏海辰科技集团 | Method for optimal seedling selection and breeding of novel carassius auratus gibelio series |
| CN101919362A (en) * | 2010-08-31 | 2010-12-22 | 中国水产科学研究院淡水渔业研究中心 | Semi-artificial breeding technology for Carassius aurutus gibelio |
| CN103563799A (en) * | 2012-08-03 | 2014-02-12 | 潘洪强 | Method for breeding allogynogenetic crucian carp fry at low temperature |
| CN104273069A (en) * | 2014-10-20 | 2015-01-14 | 苏州苏湘特种水产养殖场 | Nuisanceless allogynogenetic crucian crap breeding method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169426A (en) * | 2018-10-22 | 2019-01-11 | 广州市建波鱼苗场有限公司 | A kind of anti-season breeding method of crucian |
| CN111109160A (en) * | 2020-01-13 | 2020-05-08 | 广西壮族自治区水产引育种中心 | Method for breeding excellent variety of carp by hybridization |
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Application publication date: 20180209 |