CN109169426A - A kind of anti-season breeding method of crucian - Google Patents
A kind of anti-season breeding method of crucian Download PDFInfo
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- CN109169426A CN109169426A CN201811231675.1A CN201811231675A CN109169426A CN 109169426 A CN109169426 A CN 109169426A CN 201811231675 A CN201811231675 A CN 201811231675A CN 109169426 A CN109169426 A CN 109169426A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to a kind of anti-season breeding methods of crucian comprising following steps breed crucian in winter: (1) storage pond, holding pond and hatching tool disinfection；(2) preparation on the parent fish rearing pool；(3) parent population and its cultivation；(4) parent population is launched according to the standard of female-male proportion and corresponding screening parent population and temporarily support；(5) it hastens parturition；(6) fertilization and hatching.In Wheat summer sowing crucian: (1) storage pond, holding pond and hatching tool disinfection；(2) parent population and its cultivation；(3) parent population is launched according to female-male proportion；(4) it hastens parturition；(5) fertilization and hatching.Different propagation methods is respectively set for different seasons in the present invention, and rationally adjust water temperature, innovate ocyodinic allotment ratio, both ensured that parent population reached the physiological status of normal ovulation, sperm drainage, the height vigor of reproduction cell can be improved again, by Innovative mode and screening criteria, female fish egg aging is solved the problems, such as.
Description
Technical field
Aquaculture of the present invention is related to technical field, specifically, being a kind of anti-season breeding method of crucian.
Background technique
Crucian (CarassiusauratusLinnaeus) is distributed widely in the major rivers in the whole nation, lake, reservoir and pond
In, it is the important economic fish in China, crucian is as one of main aquatic products in China, because its delicious meat, premunition are strong, easy
Liked deeply by the majority of consumers in advantages such as raisings, there is crucian happiness grass to produce viscid egg habit, annual breed in spring season, crucian carp
Fish can be in water plant natural water body natural spawning breeding abundant.However, either any class water body such as rivers, lake, reservoir, water plant
Lack the natural spawning reproductive effect for necessarily seriously affecting carp, crucian, eventually leads to the crucian natural resources amount of these water bodys not
Disconnected decline, until exhausted state.This not only lowers the fish production power of the water bodys such as lake, reservoir, also directly influence these
The bio-diversity of water body declines.
Lake is widely known natural water area, and reservoir is the artificial water that hydraulic engineering is formed, they contain abundant
Fishery resources.For many years, lake, reservoir are that a large amount of aquatic products that market provides solve grain to abundant urban and rural residents' vegetable basket
Safety problem is eaten, creates more jobs and has played important function.However, due to many reasons, more lake eutrophication aggravation, one
A little lakes have been evolved into " algae type lake " by " plant-type lake ", and many reasons such as overfishing, the stock of fish constantly decline in addition,
The crucian quantity of the especially upper Type of Spawning of grass is fewer and fewer, individual is smaller and smaller, and resource tends to be exhausted.The reservoir manually built
General water is deeper, is not suitable for water plant growth and multiplies and serious loss, and the crucian to lay eggs on grass more lacks the condition of oviposition breeding,
Crucian resource is deficienter.
Fisherman's ligation grass bundle floats the water surface in history, although also functioning to the effect of artificial fish nest unintentionally, the purpose is to
The crucian etc. to lay eggs on trapping grass.In recent years, in order to be proliferated the stock of fish, though there is " floating type artificial fish nest ", " fixed
There are also the methods for building the simulation such as " chad artificial spawning field " fish nest and spawning ground for artificial fish nest ", however, lacking water plant in inland
The big bloom crucian resource such as lake, reservoir, how to select cheap and easy to get, the biggish artificial fish nest material of dosage, select
It is suitable for that artificial fish nest breeding blanket and scientific offering fish nest scale are set, it is accurate to grasp crucian oviposition opportunity, Artificial Fish is set in due course
Nest and safeguard that the setting place of artificial fish nest still lacks technical method from destroying and influencing etc..
Further, since under natural conditions, the optimum temperature of crucian hatching is 17~24 DEG C, but in winter or summer section low temperature and
Hot weather can frequently result in the problems such as crucian ovum rate of fertilization is low, female fish egg is easy to aging, seriously affect hatching rate, cause the summer,
Winter young crucian and adult fish shortage, price increase 40% or so.Therefore, the research and development of the anti-season breeding technique of crucian seem
It is particularly important.
Chinese patent application: CN107810889A discloses a kind of crucian method for culturing seedlings.Include the following steps that (1) breeding produces
Ovum；(2) ovum is put into hatchery uniformly to hatch, oxygenating rod is placed on pool side, and oxygenating rod is placed on bottom of pond after membrane, cultivates crucian children
Seedling；(3) it is cleaned up the pond with drug of cleaning up the pond, starts water filling, rich water after 3 days, when water color is cultivated to yellow green, crucian seedling is opened
Beginning pond-inputting；(4) it is firstly placed in oxygen bag before the pond-inputting of crucian seedling, is placed in water and buffers, be then placed in net cage；(5) it uses
Milk powder and yolk are fed as bait；Start to feed powder after (6) 12 days.
Chinese patent application: CN105875447A discloses a kind of young crucian muck cultivating method, includes the following steps, 1)
Female, milter will be put into pond in 1: 1 ratio after crucian injection ocyodinic in manual simulation's natural environment by the hatching of fish-egg
In allow its natural spawning, fertilization, hatching；Glucose is selected to breed fry as artificial induced spawning agent；2) poultry, brid guano urine and people are utilized
Fecaluria is used as the fertilizer in fry rearing pond, and fertilizer is uniformly splashed after being diluted with water muck to full pond by fermenting in advance；3) exist
A hollow place is dug on pond side, and depending on size concrete condition, then fresh excrement is poured into hole, adds suitable lime；4) in fish
It applies fertilizer daily behind pond 1 time under seedling, 50~100 kilograms per acre.But about a kind of anti-season breeding method of crucian of the present invention
It yet there are no report.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide a kind of anti-season breeding methods of crucian.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of anti-season breeding method of crucian, includes the following steps, crucian: storage pond, holding pond and hatching tool is bred in winter
Disinfection, the preparation on the parent fish rearing pool, screening parent population and its cultivation launch parent population and temporarily support, hasten parturition, being fertilized and hatching；In summer
Breeding crucian: storage pond, holding pond and the disinfection of hatching tool, screening parent population and its cultivation launch parent population, hasten parturition, being fertilized and hatching；
The method of the winter breeding crucian is as follows:
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) preparation on the parent fish rearing pool: draining pond water at the beginning of August, is exposed to the sun 2 months, and late November injects new water, water
Deep 1.2-1.5m covers water purifying tank, storage pond, holding pond, hatchery with plastic sheeting making temperature canopy, keeps in parent population the last week expected date of childbirth
Water temperature is 15-18 DEG C；
(3) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.5-0.6kg, 30-40 days before the expected date of childbirth, by qualified raun according to density be 100
Tail/mu is put into the pond rearing of depth of water 1.5m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds raun,
Once a day, 10kg/ mus each, it uses stimulation by running water 2 hours weekly,
Milter: tip fin carps in 2 ages, are reached maturity, and 1-1.5 kilograms of weight, it are put in a suitable place to breed for 80 tails/mu in room according to density
The pond rearing of outer depth of water 1.5m, and according to quality of fishmeal: peanut press pulp quality=1:1 ratio, which mixes, feeds milter, and one day one
It is secondary, it is 10kg/ mus each, even feed 20 days；
(4) it launches parent population and temporarily supports: according to standard are as follows: raun mature coefficient is 15%-20%, and milter reaches maturity, in
Hasten parturition first 2 hours and screen parent population from pond, and according to milter number: raun number=1:5 ratio is transferred to temporary in winter canopy
It supports in pond, while manufacturing water flow using the water pump of 3000-5000w in storage pond, holding pond, temperature is controlled at 15-18 DEG C；
(5) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 12 specifications be 10000 units/injection chorionic gonadotrophin, 13 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, 1g pituitary three mixed dissolution is in 50ml physiological saline for animals
In, for use；
Medicament hasten parturition 2.: by 8 specifications be 10000 units/injection chorionic gonadotrophin, 6 specifications be 0.125mg/
Both injection Luteinizing hormone releasing-hormone A2s of branch mixed dissolution is in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 50 specifications be 10000 units/injection chorionic gonadotrophin, 52 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, 2g pituitary three mixed dissolution is in 50ml physiological saline for animals
In, for use；
2) it hastens parturition implementation:
Under normal temperature condition, to raun according to injection dosage be 0.5ml/500g injection hasten parturition medicament 1., after 3h according to
2. the dosage of 0.125ml/500g hastens parturition medicament to milter injection, while the addition of whole ice is made milter storage pond, holding pond water temperature 15-18
DEG C, medicament is hastened parturition 3. to raun injection for 0.5ml/500g according to injection dosage after 3h, while raun storage pond, holding pond water temperature being kept
At 15-18 DEG C；
(6) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 15-18 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing under 10-18 DEG C of environment and is spread immediately with uniformly
It is adhered on fish nest, is placed in the spare hatchery that temperature is 15-18 DEG C；
3) sterilize for the first time: fish-egg uses the graceful disinfection of U.S. after entering pond 1.5h, removes the foam bubbled through the water column after sterilizing 2h
Finish, whole-process control water temperature is at 15-18 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Maintain water temperature at 16 DEG C；
5) after the visible fish-egg of naked eyes occur obvious stain (juvenile fish embryo's eye is reached maturity) afterwards (as shown in Figure 1), holding
Water temperature at 16 DEG C or more, until juvenile fish break ovum and out when, feed sieve that thin cooked egg is yellow, and every 1,000,000 feed through 200 mesh mesh sheet in time
For 1-2 egg yolk, every 12h is fed once, be can be transferred in pond and is raised after even feeding 2 days；
The method of the Wheat summer sowing crucian is as follows:
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.5-0.6kg；It is that 100 tails/mu specification is put in a suitable place to breed in depth of water 3m according to density
Outdoor ponds cultivate, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds raun, once a day, every time
It 10kg/ mus, even feeds 20 days,
Milter: tip fin carps in 2 ages, are reached maturity, and 1-1.5 kilograms of weight, be that 80 tails/mu is put in a suitable place to breed in water according to density
The outdoor ponds of deep 3m are cultivated, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds milter, once a day,
It 10kg/ mus every time, even feeds 20 days；
(3) parent population is launched: according to standard are as follows: raun mature coefficient is 10%-15%, and milter reaches maturity, before hastening parturition
2h screens parent population from pond and according to milter number: raun number=1:5 ratio is transferred to storage pond, holding pond, and room temperature is temporarily supported；
(4) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 8 specifications be 10000 units/branch chorionic gonadotrophin, 10 specifications are that 0.125mg/ branch promotees corpus luteum
Both hormone-releasing hormone A2 mixed dissolution is in 50ml physiological saline for animals, for use,
Medicament hasten parturition 2.: by 8 specifications be 10000 units/branch injection chorionic gonadotrophin, 6 specifications are 0.125mg/ branch
Both injection Luteinizing hormone releasing-hormone A2s mixed dissolution is in 5ml physiological saline for animals, for use,
Medicament hasten parturition 3.: by 43 specifications be 10000 units/branch injection chorionic gonadotrophin, 46 specifications are 0.125mg/
Both branch injection Luteinizing hormone releasing-hormone A2s mixed dissolution is in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Medicament is hastened parturition 1. to raun injection for 0.5ml/500g according to injection dosage under room temperature；According to injection dosage after 3h
Medicament is hastened parturition 2. to milter injection for 0.125ml/500g, while every 10m3The whole ice that quality is 90kg is added in water body, and will
Milter storage pond, holding pond water temperature is maintained at 22-24 DEG C；Medicament is hastened parturition 3. to raun injection for 0.5ml/500g according to injection dosage after 3h,
Whole ice is added simultaneously；
(5) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is quickly down to 22-23 DEG C with trash ice, it is spare；
2) milter sperm and female fish egg cell are acquired, mixing under 10-18 DEG C of environment is placed it in and is spread immediately viscous with uniformly
It invests on fish nest, is placed in the spare hatchery that temperature is 22-23 DEG C；
3) sterilize for the first time: fish-egg uses the graceful disinfection of U.S. after entering pond 1h, removes the foam bubbled through the water column after disinfection 2h dry
Only, whole ice is put into fishpond corner keeps water temperature to maintain 26 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Keep water temperature >=27 DEG C；
5) after the visible fish-egg of naked eyes occur obvious stain (juvenile fish embryo's eye is reached maturity) afterwards (as shown in Figure 1), holding
Water temperature at 27-28 DEG C, until juvenile fish break ovum and out when, feed sieve that thin cooked egg is yellow, and every 1,000,000 feed through 200 mesh mesh sheet in time
For 1-2 egg yolk, every 12h is fed once, be can be transferred in pond and is raised after even feeding 2 days.
As a preferred embodiment of the invention, implementation step of hastening parturition in the method for the winter and Wheat summer sowing crucian
The addition of whole ice is according to every 10m in rapid3The ratio addition for the whole ice that quality is 90kg is added in water body.
As a preferred embodiment of the invention, the calculation method of the mature coefficient are as follows: mature coefficient=sexual gland
Weight/weight x100%.
It is female in implementation steps of hastening parturition in the method for the Wheat summer sowing crucian as a preferred embodiment of the invention
The water temperature of fish storage pond, holding pond is maintained at 22-24 DEG C.
The invention has the advantages that:
The present invention is directed to summer respectively and winter devises reasonable crucian propagation method, by rationally adjusting water temperature, creates
New ocyodinic allotment ratio had not only ensured that parent population reached the physiological status of normal ovulation, sperm drainage, but also the height of reproduction cell can be improved
Degree vigor solves the problems, such as female fish egg aging by Innovative mode and screening criteria.Present invention innovation with higher
Property；Without using refrigeration equipments such as air-conditionings, water temperature only is adjusted with ice cube, can realize that 80% or more enters hatching rate of hatching, from
And reaches benefit and improve 30% the above object.
Detailed description of the invention
Attached drawing 1 is that juvenile fish embryo's eye is reached maturity the stage.
A indicates successful fish-egg of being fertilized in figure, the visible stain of naked eyes in transparent oophyte.B indicates unfertilized fish-egg, is white
The turbid shape of color contamination.
Specific embodiment
The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair
It is bright rather than limit the scope of the invention.In addition, it should also be understood that, after having read the content of the invention recorded, art technology
Personnel can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Fixed range.
The propagation method of embodiment 1 winter crucian
One, experimental method
[one group of experiment is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) preparation on the parent fish rearing pool: draining pond water at the beginning of August, is exposed to the sun 2 months, and late November injects new water, water
Deep 1.2-1.5m covers water purifying tank, storage pond, holding pond, hatchery with plastic sheeting making temperature canopy, keeps in parent population the last week expected date of childbirth
Water temperature is 15-18 DEG C；
(3) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.5-0.6kg, 30-40 days before the expected date of childbirth, by 100,000 qualified rauns according to density
The pond rearing of depth of water 1.5m is put into for 100 tails/mu, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio is fed
Raun, it is once a day, 10kg/ mus each, it uses stimulation by running water 2 hours weekly,
Milter: tip fin carps in 2 ages, are reached maturity, 1-1.5 kilograms of weight, by 100,000 tip fin carps according to density be 80 tails/
Mu is put in a suitable place to breed in the pond rearing of outdoor depth of water 1.5m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds hero
Fish, it is once a day, 10kg/ mus each, even feed 20 days；
(4) launch parent population and temporarily support: in hasten parturition first 2 hours by 50,000 mature coefficients be 15%-20% raun, 10,000
The milter to reach maturity is transferred in the storage pond, holding pond in winter canopy, while manufacturing water using the water pump of 3000-5000w in storage pond, holding pond
Stream, temperature are controlled at 15-18 DEG C；
(5) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 12 specifications be 10000 units/injection chorionic gonadotrophin, 13 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, pituitary 1g three mixed dissolution is in 50ml physiological saline for animals
In, for use；
Medicament hasten parturition 2.: by 8 specifications be 10000 units/injection chorionic gonadotrophin, 6 specifications be 0.125mg/
Both injection Luteinizing hormone releasing-hormone A2s of branch mixed dissolution is in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 50 specifications be 10000 units/injection chorionic gonadotrophin, 52 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, 2g pituitary three mixed dissolution is in 50ml physiological saline for animals
In, for use；
2) it hastens parturition implementation:
Under normal temperature condition, medicament is hastened parturition 1. for 0.5ml/500g injection according to injection dosage to 50,000 rauns, is pressed after 3h
Medicament is hastened parturition 2. to 10,000 milter injections according to the dosage of 0.125ml/500g, while according to every 10m3Quality is added in water body is
The ratio addition of 90kg makes 15-18 DEG C of water temperature of milter storage pond, holding pond, according to injection dosage is that 0.5ml/500g gives raun note after 3h
It penetrates and hastens parturition medicament 3., while raun storage pond, holding pond water temperature is maintained at 15-18 DEG C；
(6) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 15-18 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing under 10-18 DEG C of environment and is spread immediately with uniformly
It is adhered on fish nest, is placed in the spare hatchery that temperature is 15-18 DEG C；
3) sterilize for the first time: fish-egg uses the graceful disinfection of U.S. after entering pond 1.5h, removes the foam bubbled through the water column after sterilizing 2h
Finish, whole-process control water temperature is at 15-18 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Maintain water temperature at 16 DEG C；
5) after the visible fish-egg of naked eyes occur obvious stain (juvenile fish embryo's eye is reached maturity) afterwards (as shown in Figure 1), counting,
16 DEG C or more are maintained water temperature at, until juvenile fish can break ovum and go out after 65h, is counted, while feeding thin through 200 mesh mesh sheet sieve in time
Cooked egg is yellow, and every 1,000,000 feed as 1-2 egg yolk, and every 12h is fed once, company hello can be transferred in pond and raise after 2 days,
Spray seedling is counted.
[one group of control is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the sodium dichloro cyanurate solution for being 0.5mg/L with concentration to storage pond, holding pond and
Hatching tool soaking disinfection 24 hours；
(2) preparation on the parent fish rearing pool: draining pond water at the beginning of August, is exposed to the sun 2 months, and late November injects new water, water
Deep 1m, keeping water temperature is 10-13 DEG C；
(3) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.7-0.8kg, 15-25 days before the expected date of childbirth, by 100,000 qualified rauns according to density
The pond rearing of depth of water 1m is put into for 100 tails/mu, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:2 ratio feeds female
Fish, it is once a day, 10kg/ mus each,
Milter: tip fin carps in 2 ages, are reached maturity, 0.5-0.9 kilograms of weight, by 100,000 tip fin carps according to density be 80
Tail/mu is put in a suitable place to breed in the pond rearing of outdoor depth of water 1m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:2 ratio is fed
Milter, it is once a day, 10kg/ mus each, even feed 20 days；
(4) it launches parent population and temporarily supports: in the first 2 hours rauns by 50,000 mature coefficients for 9-14% of hastening parturition, 10,000 hairs
It is bred as ripe milter, is transferred in the storage pond, holding pond in winter canopy, while manufacturing water flow using the water pump of 3000-5000w in storage pond, holding pond,
Temperature is controlled at 10-13 DEG C；
(5) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 6 specifications be 10000 units/injection chorionic gonadotrophin, 8 specifications be 0.125mg/
Both injection Luteinizing hormone releasing-hormone A2s of branch mixed dissolution is in 50ml physiological saline for animals, for use；
Medicament hasten parturition 2.: by 6 specifications be 10000 units/injection chorionic gonadotrophin, 4 specifications be 0.125mg/
Both injection Luteinizing hormone releasing-hormone A2s of branch mixed dissolution is in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 40 specifications be 10000 units/injection chorionic gonadotrophin, 40 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, 1g pituitary three mixed dissolution is in 50ml physiological saline for animals
In, for use；
2) it hastens parturition implementation:
Under normal temperature condition, medicament is hastened parturition 1. for 0.5ml/500g injection according to injection dosage to 50,000 rauns, is pressed after 3h
Medicament is hastened parturition 2. to 10,000 milter injections according to the dosage of 0.125ml/500g, while by whole ice according to every 10m3It is added in water body
The ratio addition that quality is 90kg makes 10-13 DEG C of water temperature of milter storage pond, holding pond, gives after 3h according to injection dosage for 0.5ml/500g
3. raun injection hastens parturition medicament, while raun storage pond, holding pond water temperature is maintained at 10-13 DEG C；
(6) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 10-13 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing and uniform, distribution adherency immediately under 8 DEG C of environment
It is in 10-13 DEG C of spare hatchery on fish nest, being placed in temperature；
3) sterilize for the first time: fish-egg is sterilized after entering pond 1h with bleaching powder, removes the foam bubbled through the water column after sterilizing 2h
Finish, whole-process control water temperature is at 10-13 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Maintain water temperature at 13 DEG C；
5) it after obvious stain (juvenile fish embryo's eye is reached maturity) occurs in the visible fish-egg of naked eyes, counts, maintains water temperature at 13
DEG C, until juvenile fish break ovum and out when, count, while feeding that sieve thin cooked egg through 100 mesh mesh sheet yellow in time, every 2,000,000 feed for
1-2 egg yolk, every 12h are fed once, be can be transferred in pond and are raised after even feeding 2 days, are counted to spray seedling.
[two groups of control is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 2mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) preparation on the parent fish rearing pool: draining pond water at the beginning of August, is exposed to the sun 2 months, and late November injects new water, water
Deep 1.8-2m；
(3) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.8-0.9kg, 30-40 days before the expected date of childbirth, by 100,000 qualified rauns according to density
The pond rearing of depth of water 1.5m is put into for 100 tails/mu, and according to quality of fishmeal: the mixing of peanut press pulp quality=2:1 ratio is fed
Raun, it is once a day, 10kg/ mus each, it uses stimulation by running water 2 hours weekly,
Milter: tip fin carps in 2 ages, are reached maturity, 1.6-2 kilograms of weight, by 100,000 tip fin carps according to density be 80 tails/
Mu is put in a suitable place to breed in the pond rearing of outdoor depth of water 1.8-2m, and according to quality of fishmeal: the mixing of peanut press pulp quality=2:1 ratio is fed
Milter, it is once a day, 10kg/ mus each, even feed 20 days；
(4) it launches parent population and temporarily supports: in the first 2 hours rauns by 50,000 mature coefficients for 21-25% of hastening parturition, 10,000 hairs
It is bred as ripe milter, is transferred in the storage pond, holding pond in winter canopy, while manufacturing water flow using the water pump of 3000-5000w in storage pond, holding pond,
Temperature is controlled at 20-23 DEG C；
(5) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 12 specifications be 10000 units/fish with ovulation induction element No. 2,13 specifications be
The fish of 0.125mg/ branch ovulation induction element 3, pituitary 1g three mixed dissolution are in 50ml physiological saline for animals, for use；
Medicament hasten parturition 2.: by 8 specifications be 10000 units/fish with ovulation induction element No. 2,6 specifications be 0.125mg/
The fish of branch with ovulation induction both No. 3 mixed dissolutions of element in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 50 specifications be 10000 units/fish with ovulation induction element No. 2,52 specifications be
The fish of 0.125mg/ branch ovulation induction element 3,2g pituitary three mixed dissolution are in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Under normal temperature condition, medicament is hastened parturition 1. for 0.5ml/500g injection according to injection dosage to 50,000 rauns, is pressed after 3h
Medicament is hastened parturition 2. to 10,000 milter injections according to the dosage of 0.125ml/500g, while according to every 10m3Quality is added in water body is
Whole ice, which is added, in the ratio of 90kg makes 20-23 DEG C of water temperature of milter storage pond, holding pond, according to injection dosage is 0.5ml/500g to female after 3h
3. fish injection hastens parturition medicament, while raun storage pond, holding pond water temperature is maintained at 20-23 DEG C；
(6) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 20-23 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing under 20 DEG C of environment and is spread immediately viscous with uniformly
It invests on fish nest, is placed in the spare hatchery that temperature is 20-23 DEG C；
3) sterilize for the first time: fish-egg is sterilized after entering pond 2h with bleaching powder, removes the foam bubbled through the water column after sterilizing 2h
Finish, whole-process control water temperature is at 20-23 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Maintain water temperature at 20 DEG C；
5) it after obvious stain (juvenile fish embryo's eye is reached maturity) occurs in the visible fish-egg of naked eyes, counts, maintains water temperature at
It 20 DEG C or more, until juvenile fish breaks ovum and goes out, counts, while feeding sieve thin cooked egg Huang through 300 mesh mesh sheet in time, every 1,000,000 throw
Feeding is 3 egg yolks, and every 12h is fed once, can be transferred in pond and raises after even feeding 3 days, is counted to spray seedling.
Two, data calculate
Method is calculated as follows in the data of above-mentioned record to calculate:
Spawning rate=(ovulation weight/fish body weight) * 100%, rate of fertilization=(fertilized eggs quantity/total ovum number) * 100%, is incubated
Rate=(juvenile fish hatches number/fertilized eggs number) * 100%, emergence rate=(spray number/juvenile fish hatches number) * 100%).
Three, experimental result
(1) test 65 hours a length of when one group of hatching, when hatching of one group of control is 80 hours a length of, and two groups of control is incubated
A length of 85 hours when change.
(2) one group of spawning rate 15%, rate of fertilization 80-95%, 90% or more hatchability of fertile eggs, emergence rate are tested
98%；Compare one group of spawning rate 8%, rate of fertilization 50-60%, hatchability of fertile eggs 60%, emergence rate 70%；Compare two groups
Spawning rate 10%, rate of fertilization 70-78%, hatchability of fertile eggs 70%, emergence rate 75%.
(3) culture efficiency is assessed: one group of experiment can be improved raiser and take in 30-40%, since summer is adult fish of crucian valence
Lattice highest period, the seedling hatched at this time pass through annual growth, can list in adult fish price highest period summer next year, control one
Group can be improved raiser and take in 18-20%, and two groups of control can be improved raiser and take in 20-22%.
(4) cost and income: experiment one group of cost compared with the spring, autumn raising 15%, economic benefit can be improved 15-25% with
On, one group of cost of control improves 20% compared with spring, autumn, and 10-13% can be improved in economic benefit；Two groups of costs are compareed compared with spring, autumn
Season improves 22%, and 11-13% can be improved in economic benefit.
Four, experiment conclusion
It is above-mentioned the results showed that one group of experiment take method of the invention compared with one group of control, compare two groups of hatching when
Length, spawning rate, rate of fertilization, hatchability of fertile eggs, emergence rate are high, and culture efficiency relatively one group of control, control are two groups high.
The present invention is heated up by winter canopy induces parent population physiological function to reach oviposition level；And by optimizing reagent combination of hastening parturition
With parent fish rearing technology, achieve the purpose that anti-season breeding crucian.
The propagation method of embodiment 2 summer crucian
One, experimental method
[one group of experiment is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) parent population and its cultivation are screened:
Raun: crucian, 2 ages, weight 0.5-0.6kg；By 100,000 rauns according to density be 100 tails/mu specification by its
Put the outdoor ponds cultivation in depth of water 3m in a suitable place to breed, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds raun,
Once a day, 10kg/ mus each, even feed 20 days,
Milter: tip fin carps in 2 ages, are reached maturity, 1-1.5 kilograms of weight, by 100,000 milters according to density be 80 tails/mu
Put in a suitable place to breed the outdoor ponds cultivation in depth of water 3m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds hero
Fish, it is once a day, 10kg/ mus each, even feed 20 days；
(3) launch parent population: in the first 2 hours rauns by 50,000 mature coefficients for 15%-20% of hastening parturition, 10,000 are developed into
Ripe milter, is transferred to storage pond, holding pond, and room temperature is temporarily supported；
(4) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 8 specifications be 10000 units/branch chorionic gonadotrophin, 10 specifications are that 0.125mg/ branch promotees corpus luteum
Both hormone-releasing hormone A2 mixed dissolution is in 50ml physiological saline for animals, for use,
Medicament hasten parturition 2.: by 8 specifications be 10000 units/branch injection chorionic gonadotrophin, 6 specifications are 0.125mg/ branch
Both injection Luteinizing hormone releasing-hormone A2s mixed dissolution is in 5ml physiological saline for animals, for use,
Medicament hasten parturition 3.: by 43 specifications be 10000 units/branch injection chorionic gonadotrophin, 46 specifications are 0.125mg/
Both branch injection Luteinizing hormone releasing-hormone A2s mixed dissolution is in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Medicament is hastened parturition 1. to raun injection for 0.5ml/500g according to injection dosage under room temperature；According to injection dosage after 3h
Medicament is hastened parturition 2. to milter injection for 0.125ml/500g, while by whole ice according to every 10m3It is 90kg's that quality is added in water body
Ratio is added, and milter storage pond, holding pond water temperature is maintained at 22-24 DEG C；Raun is given to infuse for 0.5ml/500g according to injection dosage after 3h
It penetrates and hastens parturition medicament 3., while by whole ice according to every 10m3The ratio that quality is 90kg is added in water body to be added to maintain temperature to exist
22-24℃；
(5) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is quickly down to 22-23 DEG C with trash ice, it is spare；
2) milter sperm and female fish egg cell are acquired, mixing under 10-18 DEG C of environment is placed it in and is spread immediately viscous with uniformly
It invests on fish nest, is placed in the spare hatchery that temperature is 22-23 DEG C；
3) sterilize for the first time: fish-egg uses the graceful disinfection of U.S. after entering pond 1h, removes the foam bubbled through the water column after disinfection 2h dry
Only, whole ice is put into fishpond corner keeps water temperature to maintain 26 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Keep water temperature >=27 DEG C；
5) after the visible fish-egg of naked eyes occur obvious stain (juvenile fish embryo's eye is reached maturity) afterwards (as shown in Figure 1), counting,
Maintain water temperature at 16 DEG C or more, until juvenile fish can break ovum and out when, count, while fed in time through thin ripe of 200 mesh mesh sheet sieve
Egg yolk, every 1,000,000 feed as 1-2 egg yolk, and every 12h is fed once, can be transferred in pond and raise after even feeding 2 days, right
Spray seedling counts.
[one group of control is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the sodium dichloro cyanurate solution for being 0.5mg/L with concentration to storage pond, holding pond and
Hatching tool soaking disinfection 24 hours；
(2) parent population and its cultivation are screened:
Raun: 100,000 qualified rauns are put into water according to density for 100 tails/mu by crucian, 2 ages, weight 0.7-0.8kg
The pond rearing of deep 2m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:2 ratio feeds raun, once a day, every time
It 10kg/ mus, even feeds 20 days,
Milter: milter: tip fin carps in 2 ages, are reached maturity, and 0.5-0.9 kilograms of weight, by 100,000 tip fin carps according to density
It puts in a suitable place to breed for 80 tails/mu in the pond rearing of outdoor depth of water 2m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:2 ratio
Milter is fed, it is once a day, 10kg/ mus each, even feed 20 days；
(3) launch parent population: in the first 2 hours rauns by 50,000 mature coefficients for 9-14% of hastening parturition, 10,000 are reached maturity
Milter, be transferred to storage pond, holding pond, 20 DEG C are temporarily supported；
(4) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: hastening parturition medicament 1.: by 6 specifications be 10000 units/injection chorionic gonadotrophin, 8 specifications
Both injection Luteinizing hormone releasing-hormone A2s for 0.125mg/ branch mixed dissolution is in 50ml physiological saline for animals, for use；
Medicament hasten parturition 2.: by 6 specifications be 10000 units/injection chorionic gonadotrophin, 4 specifications be 0.125mg/
Both injection Luteinizing hormone releasing-hormone A2s of branch mixed dissolution is in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 40 specifications be 10000 units/injection chorionic gonadotrophin, 40 specifications be
The injection Luteinizing hormone releasing-hormone A2 of 0.125mg/ branch, 2g pituitary three mixed dissolution is in 50ml physiological saline for animals
In, for use；
2) it hastens parturition implementation:
Under normal temperature condition, medicament is hastened parturition 1. for 0.5ml/500g injection according to injection dosage to 50,000 rauns, is pressed after 3h
Medicament is hastened parturition 2. to 10,000 milter injections according to the dosage of 0.125ml/500g, while whole ice is added to make milter storage pond, holding pond water temperature
18-20 DEG C, according to injection dosage be that 3. 0.5ml/500g to raun injection hastens parturition medicament, while whole ice is added to maintain temperature after 3h
Degree is 18-20 DEG C；
(5) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is quickly down to 18 DEG C with trash ice, it is spare；
2) milter sperm and female fish egg cell are acquired, mixing under 8 DEG C of environment is placed it in and spreads and be adhered to immediately with uniformly
On fish nest, it is placed in the spare hatchery that temperature is 18 DEG C；
3) sterilize for the first time: fish-egg is sterilized after entering pond 1h using bleaching powder, removes the foam bubbled through the water column after sterilizing 2h
Completely, whole ice is put into fishpond corner keeps water temperature to maintain 22 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Keeping water temperature is 22 DEG C；
5) it after obvious stain (juvenile fish embryo's eye is reached maturity) occurs in the visible fish-egg of naked eyes, counts, maintains water temperature at
13 DEG C, juvenile fish can break ovum and out when, count, while feeding sieve that thin cooked egg is yellow, and every 2,000,000 throw through 100 mesh mesh sheet in time
Feeding is 1-2 egg yolk, and every 12h is fed once, can be transferred in pond and raises after even feeding 2 days, is counted to spray seedling.
[two groups of control is bred according to following propagation method]
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 2mg/L with concentration is to storage pond, holding pond and hatching tool leaching
Bubble disinfection 24 hours；
(2) parent population and its cultivation are screened:
Raun: 100,000 qualified rauns are put into water according to density for 100 tails/mu by crucian, 2 ages, weight 0.8-0.9kg
The pond rearing of deep 4m, and according to quality of fishmeal: the mixing of peanut press pulp quality=2:1 ratio feeds raun, once a day, every time
10kg/ mus,
Milter: tip fin carps in 2 ages, are reached maturity, 1.6-2 kilograms of weight, by 100,000 tip fin carps according to density be 80 tails/
Mu is put in a suitable place to breed in the pond rearing of outdoor depth of water 4m, and according to quality of fishmeal: the mixing of peanut press pulp quality=2:1 ratio feeds hero
Fish, it is once a day, 10kg/ mus each, even feed 20 days；
(3) it launches parent population and temporarily supports: in the first 2 hours rauns by 50,000 mature coefficients for 21-25% of hastening parturition, 10,000 hairs
It is bred as ripe milter, is transferred to storage pond, holding pond, 28 DEG C are temporarily supported；
(4) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 12 specifications be 10000 units/fish with ovulation induction element No. 2,13 specifications be
The fish of 0.125mg/ branch ovulation induction element 3, pituitary 1g three mixed dissolution are in 50ml physiological saline for animals, for use；
Medicament hasten parturition 2.: by 8 specifications be 10000 units/fish with ovulation induction element No. 2,6 specifications be 0.125mg/
The fish of branch with ovulation induction both No. 3 mixed dissolutions of element in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 50 specifications be 10000 units/fish with ovulation induction element No. 2,52 specifications be
The fish of 0.125mg/ branch ovulation induction element 3,2g pituitary three mixed dissolution are in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Under normal temperature condition, medicament is hastened parturition 1. for 0.5ml/500g injection according to injection dosage to 50,000 rauns, is pressed after 3h
Medicament is hastened parturition 2. to 10,000 milter injections according to the dosage of 0.125ml/500g, while whole ice is added to make milter storage pond, holding pond water temperature
26-28 DEG C, according to injection dosage be that 3. 0.5ml/500g to raun injection hastens parturition medicament, while it is temporary by raun that whole ice is added after 3h
It supports water temperature and is maintained at 26-28 DEG C；
(5) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 26-28 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing under 26 DEG C of environment and is spread immediately viscous with uniformly
It invests on fish nest, is placed in the spare hatchery that temperature is 26 DEG C；
3) sterilize for the first time: fish-egg is sterilized after entering pond 2h with bleaching powder, removes the foam bubbled through the water column after sterilizing 2h
Finish, whole-process control water temperature is at 26-28 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and
Maintain water temperature at 26 DEG C；
5) it after obvious stain (juvenile fish embryo's eye is reached maturity) occurs in the visible fish-egg of naked eyes, counts, maintains water temperature at
It 20 DEG C, until juvenile fish breaks ovum and goes out, counts, while feeding that sieve thin cooked egg through 300 mesh mesh sheet yellow in time, every 1,000,000 to feed be 3
A egg yolk, every 12h are fed once, be can be transferred in pond and are raised after even feeding 3 days, are counted to spray seedling.
Two, data calculate
Method is calculated as follows in the data of above-mentioned record to calculate:
Spawning rate=(ovulation weight/fish body weight) * 100%, rate of fertilization=(fertilized eggs quantity/total ovum number) * 100%, is incubated
Rate=(juvenile fish hatches number/fertilized eggs number) * 100%, emergence rate=(spray number/juvenile fish hatches number) * 100%).
Three, experimental result
(1) 45 hours a length of, spawning rate 12% when testing one group of hatching, rate of fertilization 80%, hatchability of fertile eggs 95%
More than, emergence rate 98%, 60 hours a length of, spawning rate 8% when compareing one group of hatching, rate of fertilization 60%, hatchability of fertile eggs
70%, emergence rate 78%, 65 hours a length of, spawning rate 9% when compareing two groups of hatching, rate of fertilization 65%, hatchability of fertile eggs
75%, emergence rate 68%.
(2) economic benefit: experiment one group can be improved raiser take in 30-40%, due to summer be adult fish of crucian price most
High period, the seedling hatched at this time pass through annual growth, can list in adult fish price highest period summer next year, and one group of control can
It improving raiser and takes in 18-20%, two groups of control can be improved raiser and take in 20-22%,.
(3) cost and income: experiment one group cost compared with the spring, autumn raising 15%, economic benefit can be improved 30% with
On,.Compare one group of cost and improve 26% compared with spring, autumn, economic benefit can be improved 21%, the cost of two groups of control compared with the spring,
Autumn improves 25%, and economic benefit can be improved 20%.
Four, experiment conclusion
It is above-mentioned the results showed that one group of experiment take method of the invention compared with one group of control, compare two groups of hatching when
Length, spawning rate, rate of fertilization, hatchability of fertile eggs, emergence rate are high, and culture efficiency relatively one group of control, control are two groups high.
The present invention passes through the reasonable setting of water temperature, takes reasonable cooling rate, eliminates cooling to parent population physiology machine
The influence of energy；Achieve the purpose that anti-season breeding crucian by the innovation of the innovation for reagent combination of hastening parturition, parent fish rearing technology.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, without departing from the principle of the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (5)
1. a kind of anti-season breeding method of crucian, which is characterized in that include the following steps, breed crucian: storage pond, holding pond in winter
And hatching tool sterilizes, the preparation on the parent fish rearing pool, screens parent population and its cultivation, launches parent population and temporarily support, hasten parturition, be fertilized and incubate
Change；In Wheat summer sowing crucian: storage pond, holding pond and hatching tool disinfection, screening parent population and its cultivation, launch parent population, hasten parturition, be fertilized and
Hatching；
The method of the winter breeding crucian is as follows:
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration disappears to storage pond, holding pond and hatching tool immersion
Poison 24 hours；
(2) preparation on the parent fish rearing pool: draining pond water at the beginning of August, is exposed to the sun 2 months, and late November injects new water, the depth of water
1.2-1.5m covers water purifying tank, storage pond, holding pond, hatchery with plastic sheeting making temperature canopy, keeps water in parent population the last week expected date of childbirth
Temperature is 15-18 DEG C；
(3) parent population and its cultivation are screened:
Select 2 ages, the female crucian of weight 0.5-0.6kg, 30-40 days before the expected date of childbirth, by qualified female crucian according to density be 100 tails/
Mu is put into the pond rearing of depth of water 1.5m, and according to quality of fishmeal: peanut press pulp quality=1:1 ratio, which mixes, feeds raun, and one
It is primary, 10kg/ mus each, uses stimulation by running water 2 hours weekly,
It selected for 2 ages, reach maturity, 1-1.5 kilograms of weight male tip fin carps, it is put in a suitable place to breed for 80 tails/mu in outdoor water according to density
The pond rearing of deep 1.5m, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds milter, once a day, often
It is 10kg/ mus secondary, even feed 20 days；
(4) it launches parent population and temporarily supports: according to standard are as follows: raun mature coefficient is 15%-20%, and milter reaches maturity, in hastening parturition
Parent population is screened from pond within first 2 hours, and according to milter number: raun number=1:5 ratio is transferred to the storage pond, holding pond in winter canopy
In, while water flow is manufactured using the water pump of 3000-5000w in storage pond, holding pond, temperature is controlled at 15-18 DEG C；
(5) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 12 specifications be 10000 units/injection chorionic gonadotrophin, 13 specifications be 0.125mg/ branch
Injection Luteinizing hormone releasing-hormone A2,1g pituitary three mixed dissolution in 50ml physiological saline for animals, for use；
Medicament hasten parturition 2.: by 8 specifications be 10000 units/injection chorionic gonadotrophin, 6 specifications be what 0.125mg/ was propped up
Both injection Luteinizing hormone releasing-hormone A2s mixed dissolution is in 5ml physiological saline for animals, for use；
Medicament hasten parturition 3.: by 50 specifications be 10000 units/injection chorionic gonadotrophin, 52 specifications be 0.125mg/ branch
Injection Luteinizing hormone releasing-hormone A2,2g pituitary three mixed dissolution in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Under normal temperature condition, to raun according to injection dosage be 0.5ml/500g injection hasten parturition medicament 1., after 3h according to
2. the dosage of 0.125ml/500g hastens parturition medicament to milter injection, while the addition of whole ice is made milter storage pond, holding pond water temperature 15-18
DEG C, medicament is hastened parturition 3. to raun injection for 0.5ml/500g according to injection dosage after 3h, while raun storage pond, holding pond water temperature being kept
At 15-18 DEG C；
(6) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is maintained at 15-18 DEG C, it is spare；
2) milter sperm and female fish egg cell are acquired, and places it in mixing and uniform, distribution adherency immediately under 10-18 DEG C of environment
It is in 15-18 DEG C of spare hatchery on fish nest, being placed in temperature；
3) sterilize for the first time: fish-egg uses the graceful disinfection of U.S. after entering pond 1.5h, finishes the foam bubbled through the water column removing after sterilizing 2h, entirely
Process control water temperature is at 15-18 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and keep
Water temperature is at 16 DEG C；
5) cooked egg that 200 mesh mesh sheet sieve was fed after 65h is yellow, and every 1,000,000 feed as 1-2 egg yolk, secondary/12h, even hello 2
It is transferred in pond and raises after it；
The method of the Wheat summer sowing crucian is as follows:
(1) storage pond, holding pond and hatching tool disinfection: the copper-bath for being 1mg/L with concentration disappears to storage pond, holding pond and hatching tool immersion
Poison 24 hours；
(2) parent population and its cultivation are screened:
Select 2 ages, the female crucian of weight 0.5-0.6kg；It is that 100 tails/mu specification is put in a suitable place to breed in the room of depth of water 3m according to density
Outer pond rearing, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds raun, once a day, every time
It 10kg/ mus, even feeds 20 days,
It selected for 2 ages, reach maturity, 1-1.5 kilograms of weight male tip fin carps, be that 80 tails/mu is put in a suitable place to breed in depth of water 3m according to density
Outdoor ponds cultivate, and according to quality of fishmeal: the mixing of peanut press pulp quality=1:1 ratio feeds milter, once a day, every time
It 10kg/ mus, even feeds 20 days；
(3) launch parent population: standard: raun mature coefficient 10%-15%, milter reach maturity, and 2h is sieved from pond before hastening parturition
Select parent population and according to milter number: raun number=1:5 ratio is transferred to storage pond, holding pond, and room temperature is temporarily supported；
(4) it hastens parturition:
1) preparation of ocyodinic:
Medicament hasten parturition 1.: by 8 specifications be 10000 units/branch chorionic gonadotrophin, 10 specifications are that 0.125mg/ branch luteotropin is released
Both hormone A2 mixed dissolution is put in 50ml physiological saline for animals, for use,
Medicament hasten parturition 2.: by 8 specifications be 10000 units/branch injection chorionic gonadotrophin, 6 specifications are the injection of 0.125mg/ branch
With both Luteinizing hormone releasing-hormone A2s mixed dissolution in 5ml physiological saline for animals, for use,
Medicament hasten parturition 3.: by 43 specifications be 10000 units/branch injection chorionic gonadotrophin, 46 specifications are 0.125mg/ branch note
It penetrates with both Luteinizing hormone releasing-hormone A2 mixed dissolutions in 50ml physiological saline for animals, for use；
2) it hastens parturition implementation:
Medicament is hastened parturition 1. to raun injection for 0.5ml/500g according to injection dosage under room temperature；It is according to injection dosage after 3h
2. 0.125ml/500g hastens parturition medicament to milter injection, while whole ice is added, and milter storage pond, holding pond water temperature is maintained at 22-24
℃；Medicament is hastened parturition 3. to raun injection for 0.5ml/500g according to injection dosage after 3h, while whole ice is added；
(5) fertilization and hatching:
1) enter before ovum 1h and hatchery water temperature is quickly down to 22-23 DEG C with trash ice, it is spare；
2) milter sperm and female fish egg cell are acquired, mixing under 10-18 DEG C of environment is placed it in and spreads and be adhered to immediately with uniformly
On fish nest, it is placed in the spare hatchery that temperature is 22-23 DEG C；
3) sterilize for the first time: fish-egg enters after the 1h of pond using the graceful disinfection of U.S., sterilize the foam bubbled through the water column is removed after 2h it is clean, will
Whole ice is put into fishpond corner and water temperature is kept to maintain 26 DEG C；
4) it sterilizes for second: for the first time after disinfection 12h, carrying out second and sterilize, remove the foam bubbled through the water column in time, and keep
Water temperature >=27 DEG C；
5) 200 mesh mesh sheet were fed after 45h, and to sieve thin cooked egg yellow, and every 1,000,000 feed as 1-2 egg yolk, secondary/12h, even hello
It is transferred in pond and raises after 2 days.
2. method according to claim 1, which is characterized in that implementation of hastening parturition in the method for the winter and Wheat summer sowing crucian
The addition of whole ice is according to every 10m in step3The ratio addition for the whole ice that quality is 90kg is added in water body.
3. method according to claim 1, which is characterized in that the calculation method of the mature coefficient are as follows: mature coefficient=property
Gland weight/weight x100%.
4. method according to claim 1, which is characterized in that in implementation steps of hastening parturition in the method for the Wheat summer sowing crucian
The water temperature of raun storage pond, holding pond is maintained at 22-24 DEG C.
5. method according to claim 1, which is characterized in that be in children when 45h in the method for the Wheat summer sowing crucian
Fish breaks ovum and state out；It is the state gone out in the broken ovum of juvenile fish when 65h in the method for winter breeding crucian.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114916476A (en) * | 2022-06-21 | 2022-08-19 | 广东德权渔业发展有限公司 | High-quality crucian carp breeding method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260109A (en) * | 1999-11-02 | 2000-07-19 | 华南师范大学 | Production of hybrid fingerling for high-yield crucian carp |
| CN107484682A (en) * | 2017-08-15 | 2017-12-19 | 代作光 | A kind of propagation method of carp fry |
| CN107667923A (en) * | 2017-11-01 | 2018-02-09 | 广西沙田仙人滩农业投资有限公司 | A kind of hybridized prussian carp large-scale artificial reproduction technique |
| CN108391608A (en) * | 2018-02-11 | 2018-08-14 | 平南县正达种养专业合作社 | A method of improving crucian breeding quality |
| CN108541629A (en) * | 2018-04-03 | 2018-09-18 | 湖南文理学院 | A kind of anti-season fish propagation method |
-
2018
- 2018-10-22 CN CN201811231675.1A patent/CN109169426A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260109A (en) * | 1999-11-02 | 2000-07-19 | 华南师范大学 | Production of hybrid fingerling for high-yield crucian carp |
| CN107484682A (en) * | 2017-08-15 | 2017-12-19 | 代作光 | A kind of propagation method of carp fry |
| CN107667923A (en) * | 2017-11-01 | 2018-02-09 | 广西沙田仙人滩农业投资有限公司 | A kind of hybridized prussian carp large-scale artificial reproduction technique |
| CN108391608A (en) * | 2018-02-11 | 2018-08-14 | 平南县正达种养专业合作社 | A method of improving crucian breeding quality |
| CN108541629A (en) * | 2018-04-03 | 2018-09-18 | 湖南文理学院 | A kind of anti-season fish propagation method |
Non-Patent Citations (2)
| Title |
|---|
| 力军: "反季节养鲫鱼 ", 《河南科技》 * |
| 钱丽琴: "香鱼反季节人工繁殖技术要点 ", 《中国水产》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114916476A (en) * | 2022-06-21 | 2022-08-19 | 广东德权渔业发展有限公司 | High-quality crucian carp breeding method |
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Application publication date: 20190111 |