CN101796932A - Method for optimal seedling selection and breeding of novel carassius auratus gibelio series - Google Patents

Method for optimal seedling selection and breeding of novel carassius auratus gibelio series Download PDF

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Publication number
CN101796932A
CN101796932A CN201010140103A CN201010140103A CN101796932A CN 101796932 A CN101796932 A CN 101796932A CN 201010140103 A CN201010140103 A CN 201010140103A CN 201010140103 A CN201010140103 A CN 201010140103A CN 101796932 A CN101796932 A CN 101796932A
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China
Prior art keywords
sperm
breeding
novel
carp
series
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CN201010140103A
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Chinese (zh)
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卢枳伸
徐海荣
吴健开
许金荣
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JIANGSU HAICHEN TECHNOLOGY GROUP Co Ltd
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JIANGSU HAICHEN TECHNOLOGY GROUP Co Ltd
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Priority to CN201010140103A priority Critical patent/CN101796932A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a method for optimal seedling selection and breeding of novel carassius auratus gibelio series, which comprises the following steps: (1) extracting an egg of a Fangzheng crucian carp; (2) extracting a sperm of a Xingguo red carp; (3) stimulating the female nucleus of the egg of the crucian carps for development to generate an offspring through the sperm of the Xingguo red carp; (3) extracting a sperm of a grass carp and a sperm of a megalobrama amblycephala, and extracting an egg of the offspring generated in the previous step; (5) stimulating the female nucleus of the egg of the offspring generated Step (3) for development with the sperm of the grass carp and the sperm of the megalobrama amblycephala; and (6) breeding the improved seedling. The invention has the following technical effects that: 1) the novel carassius auratus gibelio series with genetic stability can be obtained; 2) the sporozoon resistance of the bred novel carassius auratus gibelio series can be significantly improved; 3) the growth speed of the bred novel carassius auratus gibelio series is high, and the intestinal length/full length is increased, thereby facilitating the improvement of the digestibility; and 4) the thickness of the dorsal muscles and the tightness of the scales of the bred novel carassius auratus gibelio series are improved, the survival rate in the long-distance transport is more than 95 percent, the visceral mass is reduced, the body length is increased, and the intramuscular bones are decreased.

Description

The optimal seedling selection and breeding of novel carassius auratus gibelio series method
Technical field
The present invention relates to the artificial raise seedling method of a kind of fresh-water fishes, specifically, relate to a kind of optimal seedling selection and breeding of novel carassius auratus gibelio series method.
Background technology
Hybridized prussian carp is the new object of a kind of crucian cultivation that the fish breeding expert of Inst. of Hydrobiology, Chinese Academy of Sciences succeeded in developing in 1976~1981 years, it is to utilize natural gynogenetic upright pond crucian carp to be female parent, with the red carp of making the country prosperous is male parent, the filial generation of breeding through artificial insemination.This fishing gear has good hybrid vigour, and effect of increasing production is obvious, and fine and tender taste, and is nutritious, and the dried up time-to-live is long, can be under low temperature, anhydrous condition short-distance transport of living fish.The advantage that hybridized prussian carp is is to have characteristics such as growth is fast, individual greatly, strong stress resistance, demonstrates the good and economic shape in breeding production.1. feeding habits are raised hybridized prussian carp extensively, does not easily have preference to food, as long as agreeable to the taste, various foods all can utilize.2. the production of hybrid seeds is easy, and the filial generation proterties is more stable.Hybridized prussian carp is to utilize triploid upright pond crucian carp (female parent), lures that with the method for artificial hybridization the ovum of upright pond crucian carp carries out gynogenesis with the diplontic red carp (male parent) of making the country prosperous into.3. adaptability is strong, and the high hybridized prussian carp of survival rate has very strong resistance, and living accommodation is very capable.It can be put in a suitable place to breed in water body in large such as lake, reservoir, cultures in the small face that also can be surplus stream, pond etc. 100 square metre.The hybridized prussian carp hatchability of fertile eggs can reach more than 80%, and the summer flower survival rate also can reach more than 90%; Conveying method routinely, the transportation survival rate of hybridized prussian carp summer flower or adult fish can reach more than 90%.4. growth is fast, and the short hybridized prussian carp growth rate of breeding cycle is fast 34.7% than male parent, than the fast 2-3 of common crucian doubly.Generally seed can grow 200-250 gram, gram surplus maximum individual weight can reach 400 then.
But present existing hybridized prussian carp also exists certain weak point: 1, the genetic character of filial generation is stable inadequately; 2, not high enough to the sporozoit resistance; 3, growth rate is fast inadequately; 4, still exist higher lethality in the long-distance transport process.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, a kind of optimal seedling selection and breeding of novel carassius auratus gibelio series method is provided.
Optimal seedling selection and breeding of novel carassius auratus gibelio series method of the present invention comprises the following steps:
(1) extracts upright pond crucian carp ovum;
(2) extract the red carp sperm of making the country prosperous;
(3) stimulate the reproduction of pond crucian carp ovum gynogenesis to produce the gynoecy offspring with the red carp sperm of making the country prosperous as the allos sperm; In this process, the sperm of the red carp of making the country prosperous only plays inducing action, and its sperm nucleus does not merge mutually with the egg nucleus of upright pond crucian carp, and its filial generation proterties is not separated, and this just very helps go down to posterity breeding and the expansion production of hybridized prussian carp seed.
(4) extract grass carp and megalobrama amblycephala sperm, extract the filial generation ovum in (3) step;
(5) utilize grass carp and megalobrama amblycephala sperm stimulate (3) step in gynogenesis that filial generation is produced, make its filial generation proterties more stable;
(6) fine-variety breeding: adopt the method for colony's seed selection and family selective breeding combination,, intestines length/total length long with body is first and second growth traits, is aided with dorsal muscles thickness, scale elasticity, a plurality of indexs of visceral mass weight and carries out seed selection.
Preferably, in the said fine-variety breeding process, from the shoal of fish, choose individual big, body weight gain than good fish, selection rate is lower than ten thousand/, the fish of selecting is cultured, make its breeding produce fish of future generation.
More preferably, the seed selection algebraically of said fine-variety breeding process was 4 generations.
Optimal seedling selection and breeding of novel carassius auratus gibelio series method of the present invention has following technique effect:
1) can obtain to meet breeding goal, the more stable novel carassius auratus gibelio series of inheritance, set up the novel carassius auratus gibelio series seed selection of a cover standard and protect kind of a technical system, form the complete seedling artificial breeding technology system of a cover;
2) novel carassius auratus gibelio series of cultivating is significantly increased to the sporozoit resistance;
3) the novel carassius auratus gibelio series growth rate of cultivating improves 22.4%, and intestines length/total length reaches 4.2, has improved 10.5% than common hybridized prussian carp, and the enteron aisle prolongation helps improving digestibility;
4) novel carassius auratus gibelio series dorsal muscles thickness of cultivating and scale elasticity improve, and survival rate is higher than 95% in long-distance transport, and visceral mass reduces, and body is long to be increased, and thorn reduces between flesh.
Embodiment
The present invention is described in further detail below in conjunction with embodiment,
Embodiment 1:
The optimal seedling selection and breeding of novel carassius auratus gibelio series method of present embodiment comprises the following steps:
(1) the upright pond crucian carp in female Heilungkiang of choosing body weight 250 grams, 2 full ages is extracted ovum as parent population;
(2) red carp is made the country prosperous as parent population in the male Jiangxi of choosing about 1 kilogram of body weight, 2 full ages, extracts sperm;
(3) stimulate the reproduction of pond crucian carp ovum gynogenesis to produce the gynoecy offspring with the red carp sperm of making the country prosperous as the allos sperm; In this process, the sperm of the red carp of making the country prosperous only plays inducing action, and its sperm nucleus does not merge mutually with the egg nucleus of upright pond crucian carp, and its filial generation proterties is not separated, and this just very helps go down to posterity breeding and the expansion production of hybridized prussian carp seed.
(4) choose healthy and strong male grass carp and megalobrama amblycephala, extract sperm, choose the gynoecy filial generation that produces in (3) step, extract ovum;
(5) utilize grass carp and megalobrama amblycephala sperm to stimulate the gynogenesis that filial generation is produced in (3) step to produce filial generation, can make its filial generation proterties more stable;
(6) fine-variety breeding: the method that adopts colony's seed selection and family selective breeding combination,, intestines length/total length long with body is first and second growth traits, be aided with the heavy a plurality of indexs of dorsal muscles thickness, scale elasticity, visceral mass and carry out directive breeding, filter out that body is long, intestines length/total length seed selection algebraically was 4 generations.
In the fine-variety breeding process, from the shoal of fish, choose fast growth, individual big, fish that body length/body weight is good, selection rate is lower than ten thousand/, the fish of selecting is cultured, make its breeding generation fish of future generation.The seed selection algebraically of fine-variety breeding process was 4 generations.
Seed selection result: by the hybridized prussian carp shoal of fish is carried out continuous seed selection, pursue for selecting fast growth, individual big, the hybridized prussian carp that body length/body weight is good, to the 4th generation, obtain the novel carassius auratus gibelio series of stabilization characteristics of genetics, growth rate and intestines length/total length have associated effect, the relation of being proportionate, the enteron aisle prolongation helps improving digestibility, intestines length/total length reaches 4.2, has improved 10.5% than common hybridized prussian carp, and growth rate has improved 22.4% than common hybridized prussian carp, dorsal muscles thickness and scale elasticity improve, visceral mass reduces, and body is long to be increased, and thorn reduces between flesh.

Claims (3)

1. the optimal seedling selection and breeding of novel carassius auratus gibelio series method is characterized in that, comprises the following steps:
(1) extracts upright pond crucian carp ovum;
(2) extract the red carp sperm of making the country prosperous;
(3) stimulate the reproduction of pond crucian carp ovum gynogenesis to produce the gynoecy offspring with the red carp sperm of making the country prosperous as the allos sperm;
(4) extract grass carp and megalobrama amblycephala sperm, extract the filial generation ovum in (3) step;
(5) utilize grass carp and megalobrama amblycephala sperm to stimulate the filial generation sub-gynogenesis of laying eggs in (3) step;
(6) fine-variety breeding: adopt the method for colony's seed selection and family selective breeding combination,, intestines length/total length long with body is first and second growth traits, is aided with dorsal muscles thickness, scale elasticity, visceral mass weight index and carries out seed selection.
2. optimal seedling selection and breeding of novel carassius auratus gibelio series method according to claim 1, it is characterized in that, in the said fine-variety breeding process, from the shoal of fish, choose individual big, body weight gain than good fish, selection rate is lower than ten thousand/, the fish of selecting is cultured, made its breeding produce fish of future generation.
3. optimal seedling selection and breeding of novel carassius auratus gibelio series method according to claim 1 and 2 is characterized in that, the seed selection algebraically of said fine-variety breeding process was 4 generations.
CN201010140103A 2010-04-07 2010-04-07 Method for optimal seedling selection and breeding of novel carassius auratus gibelio series Pending CN101796932A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103498003A (en) * 2013-10-23 2014-01-08 中国水产科学研究院长江水产研究所 Method for detecting relative expression quantity of carassius auratus gibelio TLR9 gene with fluorescent RT-PCR
CN107667923A (en) * 2017-11-01 2018-02-09 广西沙田仙人滩农业投资有限公司 A kind of hybridized prussian carp large-scale artificial reproduction technique
CN107691309A (en) * 2017-12-05 2018-02-16 福建师范大学 A kind of capitiform character pure line breeding method of Artificial gynogenesis goldfish
CN110684777A (en) * 2019-11-13 2020-01-14 华中农业大学 Application of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular stings
CN112772468A (en) * 2020-11-27 2021-05-11 中国水产科学研究院黑龙江水产研究所 Method for breeding new species of normally-developed aponeurosis spinifera
CN115486412A (en) * 2022-05-31 2022-12-20 中国科学院水生生物研究所 Method for efficiently creating new polyploid gynogenesis clone line of silver crucian carp

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103498003A (en) * 2013-10-23 2014-01-08 中国水产科学研究院长江水产研究所 Method for detecting relative expression quantity of carassius auratus gibelio TLR9 gene with fluorescent RT-PCR
CN103498003B (en) * 2013-10-23 2015-09-30 中国水产科学研究院长江水产研究所 A kind of fluorescence RT-PCR detects the method for hybridized prussian carp TLR9 gene relative expression quantity
CN107667923A (en) * 2017-11-01 2018-02-09 广西沙田仙人滩农业投资有限公司 A kind of hybridized prussian carp large-scale artificial reproduction technique
CN107691309A (en) * 2017-12-05 2018-02-16 福建师范大学 A kind of capitiform character pure line breeding method of Artificial gynogenesis goldfish
CN107691309B (en) * 2017-12-05 2022-12-09 福州春园鲤水族有限公司 Head shape character pure line cultivation method for artificially inducing gynogenesis goldfish
CN110684777A (en) * 2019-11-13 2020-01-14 华中农业大学 Application of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular stings
CN110684777B (en) * 2019-11-13 2021-05-07 华中农业大学 Application of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular stings
CN112772468A (en) * 2020-11-27 2021-05-11 中国水产科学研究院黑龙江水产研究所 Method for breeding new species of normally-developed aponeurosis spinifera
WO2022111124A1 (en) * 2020-11-27 2022-06-02 中国水产科学研究院黑龙江水产研究所 Method for breeding novel species of normally developed fish without intermuscular bones
CN115486412A (en) * 2022-05-31 2022-12-20 中国科学院水生生物研究所 Method for efficiently creating new polyploid gynogenesis clone line of silver crucian carp
CN115486412B (en) * 2022-05-31 2024-04-02 中国科学院水生生物研究所 Method for efficiently creating new polyploid gynogenetic clone line of carassius auratus gibelio

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Application publication date: 20100811