CN107455152A - A kind of vine hard branch scion promotees to sprout the method for sterilization - Google Patents
A kind of vine hard branch scion promotees to sprout the method for sterilization Download PDFInfo
- Publication number
- CN107455152A CN107455152A CN201710757329.6A CN201710757329A CN107455152A CN 107455152 A CN107455152 A CN 107455152A CN 201710757329 A CN201710757329 A CN 201710757329A CN 107455152 A CN107455152 A CN 107455152A
- Authority
- CN
- China
- Prior art keywords
- scion
- grafting
- sterilization
- hard branch
- sprout
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
Promote to sprout the method for sterilization the invention discloses a kind of vine hard branch scion, comprise the following steps：The min of Heat thermostability 10~15 under the conditions of stiff wood scion branch is placed in into 48~55 DEG C, can carry out grafting processing after cooling.The inventive method can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to improve breeding efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves the security of nursery stock.
Description
Technical field
The present invention relates to technical field of fruit tree production, in particular it relates to which a kind of vine hard branch scion promotees to sprout the method for sterilization.
Background technology
China is the big producer of Table Grape first, with the application of vinegrowing technology and fine quality, vinegrowing
Region is expanded rapidly southwards, and South China's grape industrial benefit is significantly higher than the traditional producing region in the north, and the industry development impetus is very violent, right
The high quality seedling of improved seeds is in great demand.Graft seedling growth be grape breeding important way, the scion master of propagation by grafiting at present
If being carried out using green branch scion in the season of growth, but if with stiff wood grafting, then can by scion interim storage, grafting when
Between also can be motor-driven.After grafting, rudiment is grafting success, improves the key of breeding efficiency as early as possible for scion, but at the bud of stiff wood scion
In resting state, rudiment is slow and uneven, reduces graft seedling growth efficiency, is the principal element for limiting stiff wood scion application.In addition,
Scion is taken often to carry various cause of diseases and insect from field, easily causing disease pest by graft seedling growth propagates.
High-temperature process is the conventional processing mode of nursery stock sterilization, and releases the effective means of bud dormancy.In addition, at high temperature
Reason can also be used for preventing from adopting the latent disease outburst of rear fruit.But at present, there is not yet Heat thermostability is applied in the prior art
Improve the rudiment and sterilization of vine hard branch scion.
The content of the invention
The present invention is in order to overcome the above-mentioned deficiency of prior art, there is provided a kind of vine hard branch scion promotees to sprout the method for sterilization.
Method provided by the invention can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to carry
High breeding efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting,
Improve the security of nursery stock.
Another object of the present invention is application of the Heat thermostability in vine hard branch scion rudiment and/or sterilization is improved.
To achieve these goals, the present invention is achieved by following scheme：
The present invention has found that the resting bud breaking dormancy of scion can be stimulated by carrying out Heat thermostability to vine hard branch scion by studying,
Significantly improve the germination rate after grafting, so as to improve breeding efficiency；And can eliminates largely latent fungal pathogen and insect,
The risk that disease pest is propagated by grafting is reduced, improves the security of nursery stock.Therefore, claimed Heat thermostability
Application in vine hard branch scion rudiment and/or sterilization is improved, the condition of the Heat thermostability is 48~55 DEG C of Heat thermostabilities
10~15 min.
A kind of vine hard branch scion promotees to sprout the method for sterilization, comprises the following steps：Stiff wood scion branch is placed in 48~55
The min of Heat thermostability 10~15 under the conditions of DEG C, grafting processing can be carried out after cooling.
The method of the present invention goes for the vine hard branch scion of all kinds, and reason is all grape varieties
The hibernaculum of ripe stiff wood is in resting state, can promote termination of diapause by Heat thermostability, it is neat to improve rudiment.Meanwhile respectively
The bud of grape variety in a dormant state has very strong defensive ability/resistance ability to high temperature, and the Heat thermostability condition set by the present invention is not
Fire damage can be caused to branch.
The present invention realizes that the method for carrying out the min of Heat thermostability 10~15 under the conditions of 48~55 DEG C to stiff wood scion can have
It is a variety of, it is preferable that the present invention selects 48~55 DEG C of min of water bath condition processing stiff wood scion 10~15.
Preferably, the reaction condition of the Heat thermostability is 50 DEG C of min of Heat thermostability 10~15.
Preferably, the vine hard branch scion variety is sunlight rose.
Preferably, the stock variety used during grafting be shellfish reach, 5BB, 3309m, summer it is black.
Preferably, the vine hard branch scion is to trim the scion of leaf stem section form in pairs.
Compared with prior art, the invention has the advantages that：
（1）Heat thermostability method provided by the invention can release the bud dormancy of stiff wood scion, remarkably promote the whole of scion after grafting
Neat rudiment, improve breeding efficiency.
（2）Heat thermostability method provided by the invention can kill most of fungal disease and latent insect, improve seedling
The security of wood.
Brief description of the drawings
Fig. 1 is the flow of scion grafting and subsequent operation in embodiment.
Fig. 2 is scion grafting sprouting on different stock varieties after 50 DEG C of Heat thermostabilities and normal temperature processing in embodiment 1
Bud rate；A reaches the germination rate on stock for shellfish, and b is the germination rate on 5BB stocks, and c is the germination rate on summer black stock, and d is
Germination rate on 3309m stocks.
Embodiment
The present invention is made with reference to Figure of description and specific embodiment and further being elaborated, the embodiment
It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy
Different explanation, is conventional method；Used material is the reagent and material commercially obtained unless otherwise specified
Material.
Embodiment 1
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of sunlight rose winter pruning is gathered,
The scion of double leaf stem sections is cut into, is divided into 2 groups, is respectively placed in 50 DEG C of water-baths and about 20 DEG C of room-temperature water bath 10 min of each processing,
Scion is taken out, after cooling, scion is cut into the scion of stem-segment with single bud, carries out grafting using cleft graft, the stock variety of grafting has
Bei Da, 5BB, 3309m and summer are black, and grafting and subsequent operation flow are as shown in Figure 1.
After grafting, observe rudiment situation and count germination rate.As a result show, scion after Heat thermostability grafting in 4 anvils
It in wooden kind, can significantly do sth. in advance the sprouting of sunlight rose scion bud, and sprout neatly, germination rate is as shown in Figure 2.Treatment group is not
See the viral disorders such as leaflet, floral leaf and leaf roll, and there are the virus symptoms such as leaf roll, floral leaf, leaflet in the blade of control group early stage.
Embodiment 2
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of sunlight rose winter pruning is gathered,
The scion of double leaf stem sections is cut into, is placed in 48 DEG C of water-baths and handles 15 min, scion is taken out, after cooling, simple bud stem is cut into scion
The scion of section, grafting is carried out using cleft graft, the stock variety of grafting is 3309m, grafting and subsequent operation flow such as Fig. 1 institutes
Show.As a result find that Heat thermostability can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to
Improve breeding efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the wind that disease pest is propagated by grafting
Danger, improve the security of nursery stock.
Embodiment 3
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of summer black winter pruning is gathered, is cut into
The scion of double leaf stem sections, it is placed in 55 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion
Scion, grafting is carried out using cleft graft, the stock variety of grafting reaches for shellfish, and grafting and subsequent operation flow are as shown in Figure 1.As a result
It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve
Seedling efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves
The security of nursery stock.
Embodiment 4
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of rattan jade winter pruning is gathered, is cut into
The scion of double leaf stem sections, it is placed in 50 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion
Scion, grafting is carried out using cleft graft, the stock variety of grafting is black for the summer, and grafting and subsequent operation flow are as shown in Figure 1.As a result
It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve
Seedling efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves
The security of nursery stock.
Embodiment 5
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of the star winter pruning in east is gathered,
The scion of double leaf stem sections is cut into, is placed in 50 DEG C of water-baths and handles 10 min, scion is taken out, after cooling, simple bud stem is cut into scion
The scion of section, grafting is carried out using cleft graft, the stock variety of grafting reaches for shellfish, and grafting and subsequent operation flow are as shown in Figure 1.
As a result find that Heat thermostability can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to carry
High breeding efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting,
Improve the security of nursery stock.
Embodiment 6
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows：The annual stiff wood of the warm gram winter pruning of collection, is cut into
The scion of double leaf stem sections, it is placed in 50 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion
Scion, grafting is carried out using cleft graft, the stock variety of grafting is 5BB, and grafting and subsequent operation flow are as shown in Figure 1.As a result
It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve
Seedling efficiency；And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves
The security of nursery stock.
Claims (6)
1. application of the Heat thermostability in vine hard branch scion rudiment and/or sterilization is improved, the condition of the Heat thermostability is 48
~55 DEG C of min of Heat thermostability 10~15.
2. a kind of vine hard branch scion promotees to sprout the method for sterilization, it is characterised in that comprises the following steps：Stiff wood scion branch is put
The min of Heat thermostability 10~15 under the conditions of 48~55 DEG C, grafting processing can be carried out after cooling.
3. according to the method for claim 2, it is characterised in that the reaction condition of the Heat thermostability is 50 DEG C of Heat thermostabilities
10~15 min.
4. according to the method for claim 2, it is characterised in that the kind of the vine hard branch scion is sunlight rose.
5. according to the method for claim 2, it is characterised in that the stock variety used during grafting be shellfish reach, 5BB,
3309m, summer are black.
6. according to the method for claim 2, it is characterised in that the vine hard branch scion is trimming leaf stem section form in pairs
Scion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710757329.6A CN107455152A (en) | 2017-08-29 | 2017-08-29 | A kind of vine hard branch scion promotees to sprout the method for sterilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710757329.6A CN107455152A (en) | 2017-08-29 | 2017-08-29 | A kind of vine hard branch scion promotees to sprout the method for sterilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107455152A true CN107455152A (en) | 2017-12-12 |
Family
ID=60550549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710757329.6A Pending CN107455152A (en) | 2017-08-29 | 2017-08-29 | A kind of vine hard branch scion promotees to sprout the method for sterilization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107455152A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011019A (en) * | 2019-12-18 | 2020-04-17 | 北京三润泰克国际农业科技有限公司 | Grape grafting and seedling method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439250A (en) * | 2002-12-16 | 2003-09-03 | 穆德春 | Method for grafting hard grape scion onto green stock |
| CN101606465A (en) * | 2009-06-24 | 2009-12-23 | 华南农业大学 | A kind of method that improves the infringement of sponge gourd liriomyza sativae |
| CN103385089A (en) * | 2013-06-21 | 2013-11-13 | 抚州市临川区抚桐果蔬专业合作社 | Seedling cultivation process for Nanfeng tangerines |
| CN103416229A (en) * | 2013-08-16 | 2013-12-04 | 新疆林业科学院 | Wrapping-type walnut bark whip grafting method |
| CN103782803A (en) * | 2012-11-01 | 2014-05-14 | 长沙创远环保科技有限公司 | Greenhouse early-maturing watermelon cultivation technique |
| CN106069213A (en) * | 2016-06-20 | 2016-11-09 | 杨兆权 | A kind of method of veteran high position grafting |
-
2017
- 2017-08-29 CN CN201710757329.6A patent/CN107455152A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439250A (en) * | 2002-12-16 | 2003-09-03 | 穆德春 | Method for grafting hard grape scion onto green stock |
| CN101606465A (en) * | 2009-06-24 | 2009-12-23 | 华南农业大学 | A kind of method that improves the infringement of sponge gourd liriomyza sativae |
| CN103782803A (en) * | 2012-11-01 | 2014-05-14 | 长沙创远环保科技有限公司 | Greenhouse early-maturing watermelon cultivation technique |
| CN103385089A (en) * | 2013-06-21 | 2013-11-13 | 抚州市临川区抚桐果蔬专业合作社 | Seedling cultivation process for Nanfeng tangerines |
| CN103416229A (en) * | 2013-08-16 | 2013-12-04 | 新疆林业科学院 | Wrapping-type walnut bark whip grafting method |
| CN106069213A (en) * | 2016-06-20 | 2016-11-09 | 杨兆权 | A kind of method of veteran high position grafting |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011019A (en) * | 2019-12-18 | 2020-04-17 | 北京三润泰克国际农业科技有限公司 | Grape grafting and seedling method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103299812B (en) | Apocarya hardwood cutting seedling raising method | |
| CN104082148B (en) | The method of iris aseptic bennet regeneration expanding propagation | |
| JP6043117B2 (en) | New tomato multi-tailored grafted seedling and method for producing the same | |
| KR101523436B1 (en) | Yulgok plum tree propagation method and the method consisting of lush plum tree seedlings Yulgok | |
| Eftekhari et al. | In vitro propagation of four Iranian grape varieties: influence of genotype and pretreatment with arbuscular mycorrhiza | |
| CN103120098A (en) | Grafting method capable of rapidly fixing incomplete crown of fruit tree | |
| Syamal et al. | Performance of wedge grafting in guava under different growing conditions | |
| CN106069674A (en) | A kind of method for quickly breeding being applicable to multiple Kiwifruit Cultivars | |
| JPWO2014073595A1 (en) | Cultivation method of crops by grafting using seeds | |
| CN105684811A (en) | Method for producing winter-period fruits of atemoya | |
| CN102668984A (en) | Method for pear blade inducing adventitious buds to regenerate plant | |
| CN104285684A (en) | Method for grafting prunus cerasifera onto wild peach stock | |
| Marelli et al. | Infection biology of Moniliophthora perniciosa on Theobroma cacao and alternate solanaceous hosts | |
| CN108076881A (en) | Citrus winter heats method for culturing seedlings | |
| CN105993623A (en) | Grafting method for cherry tomatoes | |
| Marks | Rhododendron cuttings. I. Improved rooting following ‘rejuvenation’in vitro | |
| CN107455152A (en) | A kind of vine hard branch scion promotees to sprout the method for sterilization | |
| CN107018804A (en) | A kind of breeding method of tomato panax ginseng fruit | |
| CN105052569A (en) | Method for realizing shield bud grafting with wood of seedling | |
| CN106613993A (en) | Culture method of tissue culture regeneration seedlings of trifoliate oranges | |
| CN104115752A (en) | Tissue culture propagation method of taxodium hybrids'zhongshansha118' | |
| CN106489748A (en) | A kind of quick breeding by group culture method of wintergreen | |
| CN1305371C (en) | Tufted bamboo tissue culture rapid breeding rooting method | |
| CN106856996B (en) | Grafting cultivation method for tobacco | |
| Qin et al. | Effect of preharvest bagging on fruit epidermis epiphyte community structure of ‘Red Fuji’apple |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171212 |
|
| RJ01 | Rejection of invention patent application after publication |