CN107455152A - A kind of vine hard branch scion promotees to sprout the method for sterilization - Google Patents

A kind of vine hard branch scion promotees to sprout the method for sterilization Download PDF

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Publication number
CN107455152A
CN107455152A CN201710757329.6A CN201710757329A CN107455152A CN 107455152 A CN107455152 A CN 107455152A CN 201710757329 A CN201710757329 A CN 201710757329A CN 107455152 A CN107455152 A CN 107455152A
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CN
China
Prior art keywords
scion
grafting
sterilization
hard branch
sprout
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Pending
Application number
CN201710757329.6A
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Chinese (zh)
Inventor
黄旭明
王惠聪
谢斌霞
严婷婷
李志远
梁晓文
周颖怡
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South China Agricultural University
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South China Agricultural University
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Publication date
Application filed by South China Agricultural University filed Critical South China Agricultural University
Priority to CN201710757329.6A priority Critical patent/CN107455152A/en
Publication of CN107455152A publication Critical patent/CN107455152A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Promote to sprout the method for sterilization the invention discloses a kind of vine hard branch scion, comprise the following steps:The min of Heat thermostability 10~15 under the conditions of stiff wood scion branch is placed in into 48~55 DEG C, can carry out grafting processing after cooling.The inventive method can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to improve breeding efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves the security of nursery stock.

Description

A kind of vine hard branch scion promotees to sprout the method for sterilization
Technical field
The present invention relates to technical field of fruit tree production, in particular it relates to which a kind of vine hard branch scion promotees to sprout the method for sterilization.
Background technology
China is the big producer of Table Grape first, with the application of vinegrowing technology and fine quality, vinegrowing Region is expanded rapidly southwards, and South China's grape industrial benefit is significantly higher than the traditional producing region in the north, and the industry development impetus is very violent, right The high quality seedling of improved seeds is in great demand.Graft seedling growth be grape breeding important way, the scion master of propagation by grafiting at present If being carried out using green branch scion in the season of growth, but if with stiff wood grafting, then can by scion interim storage, grafting when Between also can be motor-driven.After grafting, rudiment is grafting success, improves the key of breeding efficiency as early as possible for scion, but at the bud of stiff wood scion In resting state, rudiment is slow and uneven, reduces graft seedling growth efficiency, is the principal element for limiting stiff wood scion application.In addition, Scion is taken often to carry various cause of diseases and insect from field, easily causing disease pest by graft seedling growth propagates.
High-temperature process is the conventional processing mode of nursery stock sterilization, and releases the effective means of bud dormancy.In addition, at high temperature Reason can also be used for preventing from adopting the latent disease outburst of rear fruit.But at present, there is not yet Heat thermostability is applied in the prior art Improve the rudiment and sterilization of vine hard branch scion.
The content of the invention
The present invention is in order to overcome the above-mentioned deficiency of prior art, there is provided a kind of vine hard branch scion promotees to sprout the method for sterilization. Method provided by the invention can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to carry High breeding efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, Improve the security of nursery stock.
Another object of the present invention is application of the Heat thermostability in vine hard branch scion rudiment and/or sterilization is improved.
To achieve these goals, the present invention is achieved by following scheme:
The present invention has found that the resting bud breaking dormancy of scion can be stimulated by carrying out Heat thermostability to vine hard branch scion by studying, Significantly improve the germination rate after grafting, so as to improve breeding efficiency;And can eliminates largely latent fungal pathogen and insect, The risk that disease pest is propagated by grafting is reduced, improves the security of nursery stock.Therefore, claimed Heat thermostability Application in vine hard branch scion rudiment and/or sterilization is improved, the condition of the Heat thermostability is 48~55 DEG C of Heat thermostabilities 10~15 min.
A kind of vine hard branch scion promotees to sprout the method for sterilization, comprises the following steps:Stiff wood scion branch is placed in 48~55 The min of Heat thermostability 10~15 under the conditions of DEG C, grafting processing can be carried out after cooling.
The method of the present invention goes for the vine hard branch scion of all kinds, and reason is all grape varieties The hibernaculum of ripe stiff wood is in resting state, can promote termination of diapause by Heat thermostability, it is neat to improve rudiment.Meanwhile respectively The bud of grape variety in a dormant state has very strong defensive ability/resistance ability to high temperature, and the Heat thermostability condition set by the present invention is not Fire damage can be caused to branch.
The present invention realizes that the method for carrying out the min of Heat thermostability 10~15 under the conditions of 48~55 DEG C to stiff wood scion can have It is a variety of, it is preferable that the present invention selects 48~55 DEG C of min of water bath condition processing stiff wood scion 10~15.
Preferably, the reaction condition of the Heat thermostability is 50 DEG C of min of Heat thermostability 10~15.
Preferably, the vine hard branch scion variety is sunlight rose.
Preferably, the stock variety used during grafting be shellfish reach, 5BB, 3309m, summer it is black.
Preferably, the vine hard branch scion is to trim the scion of leaf stem section form in pairs.
Compared with prior art, the invention has the advantages that:
(1)Heat thermostability method provided by the invention can release the bud dormancy of stiff wood scion, remarkably promote the whole of scion after grafting Neat rudiment, improve breeding efficiency.
(2)Heat thermostability method provided by the invention can kill most of fungal disease and latent insect, improve seedling The security of wood.
Brief description of the drawings
Fig. 1 is the flow of scion grafting and subsequent operation in embodiment.
Fig. 2 is scion grafting sprouting on different stock varieties after 50 DEG C of Heat thermostabilities and normal temperature processing in embodiment 1 Bud rate;A reaches the germination rate on stock for shellfish, and b is the germination rate on 5BB stocks, and c is the germination rate on summer black stock, and d is Germination rate on 3309m stocks.
Embodiment
The present invention is made with reference to Figure of description and specific embodiment and further being elaborated, the embodiment It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy Different explanation, is conventional method;Used material is the reagent and material commercially obtained unless otherwise specified Material.
Embodiment 1
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of sunlight rose winter pruning is gathered, The scion of double leaf stem sections is cut into, is divided into 2 groups, is respectively placed in 50 DEG C of water-baths and about 20 DEG C of room-temperature water bath 10 min of each processing, Scion is taken out, after cooling, scion is cut into the scion of stem-segment with single bud, carries out grafting using cleft graft, the stock variety of grafting has Bei Da, 5BB, 3309m and summer are black, and grafting and subsequent operation flow are as shown in Figure 1.
After grafting, observe rudiment situation and count germination rate.As a result show, scion after Heat thermostability grafting in 4 anvils It in wooden kind, can significantly do sth. in advance the sprouting of sunlight rose scion bud, and sprout neatly, germination rate is as shown in Figure 2.Treatment group is not See the viral disorders such as leaflet, floral leaf and leaf roll, and there are the virus symptoms such as leaf roll, floral leaf, leaflet in the blade of control group early stage.
Embodiment 2
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of sunlight rose winter pruning is gathered, The scion of double leaf stem sections is cut into, is placed in 48 DEG C of water-baths and handles 15 min, scion is taken out, after cooling, simple bud stem is cut into scion The scion of section, grafting is carried out using cleft graft, the stock variety of grafting is 3309m, grafting and subsequent operation flow such as Fig. 1 institutes Show.As a result find that Heat thermostability can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to Improve breeding efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the wind that disease pest is propagated by grafting Danger, improve the security of nursery stock.
Embodiment 3
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of summer black winter pruning is gathered, is cut into The scion of double leaf stem sections, it is placed in 55 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion Scion, grafting is carried out using cleft graft, the stock variety of grafting reaches for shellfish, and grafting and subsequent operation flow are as shown in Figure 1.As a result It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve Seedling efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves The security of nursery stock.
Embodiment 4
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of rattan jade winter pruning is gathered, is cut into The scion of double leaf stem sections, it is placed in 50 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion Scion, grafting is carried out using cleft graft, the stock variety of grafting is black for the summer, and grafting and subsequent operation flow are as shown in Figure 1.As a result It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve Seedling efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves The security of nursery stock.
Embodiment 5
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of the star winter pruning in east is gathered, The scion of double leaf stem sections is cut into, is placed in 50 DEG C of water-baths and handles 10 min, scion is taken out, after cooling, simple bud stem is cut into scion The scion of section, grafting is carried out using cleft graft, the stock variety of grafting reaches for shellfish, and grafting and subsequent operation flow are as shown in Figure 1. As a result find that Heat thermostability can stimulate the resting bud breaking dormancy of scion, significantly improve the germination rate after grafting, so as to carry High breeding efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, Improve the security of nursery stock.
Embodiment 6
A kind of vine hard branch scion promotees to sprout the method for sterilization, and step is as follows:The annual stiff wood of the warm gram winter pruning of collection, is cut into The scion of double leaf stem sections, it is placed in 50 DEG C of water-baths and handles 10 min, takes out scion, after cooling, stem-segment with single bud is cut into scion Scion, grafting is carried out using cleft graft, the stock variety of grafting is 5BB, and grafting and subsequent operation flow are as shown in Figure 1.As a result It was found that Heat thermostability can stimulate the resting bud breaking dormancy of scion, the germination rate after grafting is significantly improved, is educated so as to improve Seedling efficiency;And can eliminates largely latent fungal pathogen and insect, reduces the risk that disease pest is propagated by grafting, improves The security of nursery stock.

Claims (6)

1. application of the Heat thermostability in vine hard branch scion rudiment and/or sterilization is improved, the condition of the Heat thermostability is 48 ~55 DEG C of min of Heat thermostability 10~15.
2. a kind of vine hard branch scion promotees to sprout the method for sterilization, it is characterised in that comprises the following steps:Stiff wood scion branch is put The min of Heat thermostability 10~15 under the conditions of 48~55 DEG C, grafting processing can be carried out after cooling.
3. according to the method for claim 2, it is characterised in that the reaction condition of the Heat thermostability is 50 DEG C of Heat thermostabilities 10~15 min.
4. according to the method for claim 2, it is characterised in that the kind of the vine hard branch scion is sunlight rose.
5. according to the method for claim 2, it is characterised in that the stock variety used during grafting be shellfish reach, 5BB, 3309m, summer are black.
6. according to the method for claim 2, it is characterised in that the vine hard branch scion is trimming leaf stem section form in pairs Scion.
CN201710757329.6A 2017-08-29 2017-08-29 A kind of vine hard branch scion promotees to sprout the method for sterilization Pending CN107455152A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111011019A (en) * 2019-12-18 2020-04-17 北京三润泰克国际农业科技有限公司 Grape grafting and seedling method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1439250A (en) * 2002-12-16 2003-09-03 穆德春 Method for grafting hard grape scion onto green stock
CN101606465A (en) * 2009-06-24 2009-12-23 华南农业大学 A kind of method that improves the infringement of sponge gourd liriomyza sativae
CN103385089A (en) * 2013-06-21 2013-11-13 抚州市临川区抚桐果蔬专业合作社 Seedling cultivation process for Nanfeng tangerines
CN103416229A (en) * 2013-08-16 2013-12-04 新疆林业科学院 Wrapping-type walnut bark whip grafting method
CN103782803A (en) * 2012-11-01 2014-05-14 长沙创远环保科技有限公司 Greenhouse early-maturing watermelon cultivation technique
CN106069213A (en) * 2016-06-20 2016-11-09 杨兆权 A kind of method of veteran high position grafting

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1439250A (en) * 2002-12-16 2003-09-03 穆德春 Method for grafting hard grape scion onto green stock
CN101606465A (en) * 2009-06-24 2009-12-23 华南农业大学 A kind of method that improves the infringement of sponge gourd liriomyza sativae
CN103782803A (en) * 2012-11-01 2014-05-14 长沙创远环保科技有限公司 Greenhouse early-maturing watermelon cultivation technique
CN103385089A (en) * 2013-06-21 2013-11-13 抚州市临川区抚桐果蔬专业合作社 Seedling cultivation process for Nanfeng tangerines
CN103416229A (en) * 2013-08-16 2013-12-04 新疆林业科学院 Wrapping-type walnut bark whip grafting method
CN106069213A (en) * 2016-06-20 2016-11-09 杨兆权 A kind of method of veteran high position grafting

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111011019A (en) * 2019-12-18 2020-04-17 北京三润泰克国际农业科技有限公司 Grape grafting and seedling method

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Application publication date: 20171212

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