CN107347611A - A kind of red sage root soilless culture method - Google Patents

A kind of red sage root soilless culture method Download PDF

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Publication number
CN107347611A
CN107347611A CN201710764113.2A CN201710764113A CN107347611A CN 107347611 A CN107347611 A CN 107347611A CN 201710764113 A CN201710764113 A CN 201710764113A CN 107347611 A CN107347611 A CN 107347611A
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China
Prior art keywords
matrix
red sage
sage root
root
cultivation matrix
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CN201710764113.2A
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Chinese (zh)
Inventor
卢丽兰
魏建和
何春娥
杨成民
杨新全
周亚奎
符式龙
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Institute of Medicinal Plant Development of CAMS and PUMC
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Institute of Medicinal Plant Development of CAMS and PUMC
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Priority to CN201710764113.2A priority Critical patent/CN107347611A/en
Publication of CN107347611A publication Critical patent/CN107347611A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention belongs to Chinese herbal medicine field of planting, is related to a kind of red sage root soilless culture method, is that organic substrate, frog stone, perlite mixed preparing are obtained into cultivation matrix, standby;Vernalization is carried out in greenhouse after taking the root segment of the red sage root, sterilization, the root segment of budding is transplanted into crop field carries out nursery, when seedling length to 3~4 true leaves, you can is transplanted in the planting pot for loading cultivation matrix, per one plant of cave;Water and fertilizer management is carried out in each growth phase of the red sage root.The present invention is different according to requirement of each growth and development stage of the red sage root to moisture and nutrient, scientific and reasonable management is carried out to it, use the mode of soilless culture, do not limited by soil, soil condition, planting range can be expanded, accelerate plant growth, improve yield, and liquid manure dosage can be reduced, reduce production cost, it is ensured that medicinal material is real organic free of contamination Chinese herbal medicine, improves red sage root quality.

Description

A kind of red sage root soilless culture method
Technical field
The invention belongs to Chinese herbal medicine field of planting, is related to a kind of cultural method of the red sage root, especially a kind of red sage root soilless culture Method.
Background technology
The red sage root, enter its medicinal dry root and rhizome for labiate.It is inducing meastruation to relieve menalgia with promoting blood circulation, relieving restlessness that clears away heart-fire, The effect of cool blood to disappear carbuncle.For chest impediment and cardialgia, gastral cavity abdomen hypochondriac pain, hot numbness pain, dysphoria and insomnia, irregular menstruation, dysmenorrhoea Amenorrhea, sore Swelling and pain etc., are parts of generic medicinal plants.
The red sage root is all distributed in national most area, wild Salvia miltiorrhiza be typically born in endroit thick grass, limes marginis, roadside or The ground such as woods side.With the development of cultivation technique, the yield of the artificial cultivation red sage root greatly improves, and meets the needs of market is to the red sage root. At present, existing red sage root implantation methods are mainly root division method, cutting propagation method and the numerous plant method of seed.No matter which kind of plantation side Formula, the limitation of equal climate and edaphic condition, yield and quality are also inconsistent.
The content of the invention
It is an object of the invention to provide a kind of red sage root soilless culture method, solves red sage root cultivation by soil, soil condition The problem of limitation, have the advantages that pest and disease damage is small, yield is high, quality is high, cultivation is provided for salvia miltiorrhiza nutrition experimental study material Culture method.
The technical solution adopted in the present invention:
A kind of red sage root soilless culture method, it is comprised the following steps that:
1st, cultivation matrix is prepared
By organic substrate, frog stone, perlite by volume 1:1:1 ratio mixed preparing obtains cultivation matrix, standby.Institute It is to take turfy soil and red sage root to extract waste residue respectively to state organic substrate, by volume 1 after crushing:1 ratio mixing, is then carried out In 4~June of anaerobic fermentation, sterilized, then covered with covering, after 3~4 weeks with the formalin that mass fraction is 30% after fermentation Covering is opened, is tedded, it is standby.
2nd, root segment culturing and transplanting seedlings
Take the root of the red sage root to be cut into 5~8cm segment, vernalization, sand bed are carried out using layer sand cladding process in greenhouse after sterilization The root segment of budding is transplanted to crop field and carries out nursery, when seedling length to 3~4 true leaves, you can will by heat and moisture preserving until budding It is transplanted in the planting pot for loading cultivation matrix, per one plant of cave.
3rd, water and fertilizer management
It is respectively in the watering frequency and dose of each growth phase:
During seedling-slowing stage (in 20 days in growth phase), watered when cultivation matrix water content is less than 60%, irrigation amount is 200ml/L matrix, dose are 350~365mg/L matrix, and Effects of Fertilization Frequency is 1 times/week;
During Seedling Stage (the 21st~40 day in growth phase), water, water when cultivation matrix water content is less than 65% It is 350~365mg/L matrix to measure as 200ml/L matrix, dose, and Effects of Fertilization Frequency is 1 times/week;
During the strong sprout phase (the 41st~60 day in growth phase), water, water when cultivation matrix water content is less than 65% It is 420~430mg/L matrix to measure as 200ml/L matrix, dose, and Effects of Fertilization Frequency is 1 times/week;
During florescence (the 61st~75 day in growth phase), water, water when cultivation matrix water content is less than 62% It is 420~430mg/L matrix to measure as 200ml/L matrix, dose, and Effects of Fertilization Frequency is 1 times/week;
Expand to (the 76th~120 day in growth phase) during the maturity period, poured when cultivation matrix water content is less than 62% Water, irrigation amount are 200ml/L matrix, and dose is 425~435mg/L matrix, and Effects of Fertilization Frequency is 1 times/week, you can harvest.
The fertilizer described above that use that applies fertilizer is nitrogen phosphoris and potassium fertilizer ratio for 2:0.25:2 composite fertilizer.
The advantages and positive effects of the present invention are:
The present invention is different according to requirement of each growth and development stage of the red sage root to moisture and nutrient, it is carried out scientific and reasonable Management.Mixed fermentation is carried out using turfy soil and red sage root extraction waste residue, abundant nutrition and organic fertilizer can be provided, it is also fully sharp The effective component and organic substance remained in waste residue is extracted with red sage root, is effectively improved matrix structure, while provide nutritional ingredient The red sage root is given with active principle to absorb, and using the mode of soilless culture, can be expanded planting range, be accelerated plant growth, improve production Amount, save artificial, it is ensured that medicinal material is real organic free of contamination Chinese herbal medicine.Compared with traditional cultural method, the present invention Do not limited by soil, soil condition, can make plant strain growth speed faster, growing way it is more healthy and stronger, and can reduce liquid manure dosage, reduce life Cost is produced, improves red sage root quality.
Embodiment
Below by specific embodiment, the invention will be further described, and following examples are descriptive, is not limit Qualitatively, it is impossible to which protection scope of the present invention is limited with this.
Turfy soil and red sage root is taken to extract waste residue respectively, by volume 1 after crushing:1 ratio mixing, then carries out anaerobic Ferment May, sterilized after fermentation with the formalin that mass fraction is 30%, then covered with covering (plastic cloth), taken off after 4 weeks Open covering thing, is tedded, and obtains organic substrate, standby.
Take the root of the red sage root to be cut into 5~8cm segment, vernalization, sand bed are carried out using layer sand cladding process in greenhouse after sterilization The root segment of budding is transplanted to crop field and carries out nursery, treat seedling length to 3~4 true leaves (about 5cm is high) by heat and moisture preserving until budding When, you can it is transplanted in the planting pot for loading cultivation matrix, per one plant of cave.
Embodiment one, matrix components experiment
This experiment sets five cultivation matrixes:Cultivation matrix A:Organic substrate and vermiculite are with volume ratio 1:1 ratio forms;Cultivation Matrix B:Organic substrate and perlite are with volume ratio 1:1 ratio forms;Cultivation matrix C:Organic substrate, vermiculite and perlite are with body Product ratio 1:1:1 ratio forms;Cultivation matrix D:Vermiculite and perlite are with volume ratio 1:1 ratio forms;Cultivation matrix E:Soil is Matrix.
Each cultivation matrix repeats six times.Red sage root seedling after being transplanted on cultivation matrix carries out growth management, various conditions All same.It should be noted that the fertilizer used that applied fertilizer in the present embodiment is nitrogen phosphoris and potassium fertilizer ratio for 2:0.25:2 composite fertilizer.
In the maturity period of the red sage root, measurement records its root footpath, root dry weight, radical growth indexes finally take statistics and compared.Simultaneously To fat-soluble (total-tanshinone) in red sage root and water-soluble substances (tanshin polyphenolic acid B) assay.It the results are shown in Table 1.
Salvia miltiorrhiza Growth and the index of quality under the conditions of 1 different cultivation matrixes of table
The above results show that the growth indexes of the red sage root and quality are different under the conditions of different cultivation matrixes, wherein, cultivation Matrix C processing behaves oneself best, and is secondly matrix B and matrix A.
It can be seen from result of the test, by organic substrate (turfy soil and the red sage root extract waste residue), vermiculite, perlite volume ratio 1:1:Growth and development of the cultivation matrix of 1 mixed preparing for the red sage root more have facilitation.
Embodiment two, fertilizer components experiment
This experiment sets six formulas, that is, is formulated A, formula B, formula C, formula D, formula E, formula F, formula G and control CK, Various composition ratio is shown in Table 2 in fertilizer formula, and control CK is from classical Huo Gelan (Hoagland) formula fertilizer.
The fertilizer ratio of table 2
Formula Ca(NO3)2.4H2O NaH2PO4 K2SO4 KCl MgSO4.7H2O N:P:K
A 0 0.25 0.9 0.2 0.65 0:0.25:2
B 1.0 0.25 0.9 0.2 0.65 2:0.25:2
C 5.0 0.25 0.9 0.2 0.65 10:0.25:2
D 1.0 0 0.9 0.2 0.65 2:0:2
E 1.0 2.0 0.9 0.2 0.65 2:2:2
F 1.0 0.25 0 0 0.65 2:0.25:0
G 1.0 0.25 3.6 0.4 0.65 2:0.25:8
Each formula repeats six times.Red sage root seedling after being transplanted on cultivation matrix carries out growth management, and various conditions are pressed The condition of the present invention is implemented.In the maturation of the red sage root, upgrowth situation that observation Different Nutrition formula is irrigated, measurement record its root footpath, Root dry weight, radical, which finally take statistics, to be compared.To fat-soluble (total-tanshinone) in red sage root and water-soluble substances (tanshin polyphenolic acid B) content Measure.It the results are shown in Table 3.
To Salvia miltiorrhiza Growth and index of quality contrast table under the Different Nutrition of table 3 formula irrigation conditions
It is above-mentioned test result indicates that, in the case where other conditions are equal, be formulated B growth indexes and content of drug effect components Behave oneself best, i.e. N in fertilizer:P:K=2:0.25:The 2 ratio optimum present invention provides growth and the hair of the soilless culture red sage root Educate.
Embodiment three, water and fertilizer management experiment
Under greenhouse experiment, water and fertilizer amount management are imposed to the red sage root for being transplanted to cultivation matrix, operated three times altogether, Labeled as operation 1, operation 2, operation 3, concrete operations index is shown in Table 4, table 5, table 6.
Red sage root water and fertilizer amount management in the operation of table 41
Red sage root water and fertilizer amount management in the operation of table 52
Red sage root water and fertilizer amount management in the operation of table 63
Wherein, irrigation amount is 200mg/L matrix, and Effects of Fertilization Frequency is to apply fertilizer weekly once.
Control:
Under greenhouse experiment, water and fertilizer amount management are imposed to the red sage root for being transplanted to cultivation matrix, concrete operations index is shown in Table 7:
The red sage root water of table 7 and fertilizer amount management
Wherein, irrigation amount is 200mg/L matrix;Effects of Fertilization Frequency is to apply fertilizer weekly once.
Using conventional soilless culture method as control, compared with red sage root soilless culture method provided by the invention. The maturation of the red sage root, the upgrowth situation that observation Different Nutrition formula is irrigated, measurement records its root footpath, root dry weight, radical are finally united Meter compares.To fat-soluble (total-tanshinone) in red sage root and water-soluble substances (tanshin polyphenolic acid B) assay.It the results are shown in Table 8.
The influence that 8 different cultural methods of table are developed to Salvia miltiorrhiza Growth
From the result of 8 tables, promotion of the soilless culture method provided by the invention to the growth and development state of the red sage root is made With being substantially better than conventional cultural method.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, without departing from the technical principles of the invention, some improvement, retouching and color change can also be made, these Improve, retouching and color change also should be regarded as protection scope of the present invention.

Claims (1)

1. a kind of red sage root soilless culture method, it is characterised in that it is comprised the following steps that:
1) cultivation matrix, is prepared
By organic substrate, frog stone, perlite by volume 1:1:1 ratio mixed preparing obtains cultivation matrix, standby;It is described to have Machine matrix is to take turfy soil and red sage root to extract waste residue respectively, by volume 1 after crushing:1 ratio mixing, then carries out anaerobic Ferment for 4~June, sterilized after fermentation with the formalin that mass fraction is 30%, then covered with covering, opened after 3~4 weeks Covering, ted, it is standby;
2), root segment culturing and transplanting seedlings
Take the root of the red sage root to be cut into 5~8cm segment, vernalization, sand bed insulation are carried out using layer sand cladding process in greenhouse after sterilization The root segment of budding is transplanted to crop field and carries out nursery, when seedling length to 3~4 true leaves, you can moved by moisturizing until budding Plant in the planting pot for loading cultivation matrix, per one plant of cave;
3), water and fertilizer management
It is respectively in the watering frequency and dose of each growth phase:
During seedling-slowing stage, watered when cultivation matrix water content is less than 60%, irrigation amount be 200ml/L matrix, dose for 350~ 365mg/L matrix, Effects of Fertilization Frequency are 1 times/week;
During Seedling Stage, watered when cultivation matrix water content is less than 65%, irrigation amount be 200ml/L matrix, dose for 350~ 365mg/L matrix, Effects of Fertilization Frequency are 1 times/week;
During the strong sprout phase, watered when cultivation matrix water content is less than 65%, irrigation amount be 200ml/L matrix, dose for 420~ 430mg/L matrix, Effects of Fertilization Frequency are 1 times/week;
During florescence, watered when cultivation matrix water content is less than 62%, irrigation amount be 200ml/L matrix, dose for 420~ 430mg/L matrix, Effects of Fertilization Frequency are 1 times/week;
When expanding to the maturity period, watered when cultivation matrix water content is less than 62%, irrigation amount is 200ml/L matrix, dose For 425~435mg/L matrix, Effects of Fertilization Frequency is 1 times/week, you can harvest;
The fertilizer described above that use that applies fertilizer is nitrogen phosphoris and potassium fertilizer ratio for 2:0.25:2 composite fertilizer.
CN201710764113.2A 2017-08-30 2017-08-30 A kind of red sage root soilless culture method Withdrawn CN107347611A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108432592A (en) * 2018-04-19 2018-08-24 闽南师范大学 A kind of novel greening seedling matrix
CN109717038A (en) * 2019-03-13 2019-05-07 四川茂承生物科技有限公司 Radix Salviae Miltiorrhizae high quality seedling breeding method
CN111631113A (en) * 2020-07-10 2020-09-08 南京中医药大学 Soilless seedling culture medium for salvia miltiorrhiza
CN112166844A (en) * 2020-11-11 2021-01-05 山东农业大学 Salvia miltiorrhiza seedling propagation method for vegetative propagation by using root segment pregermination
CN113615539A (en) * 2020-05-07 2021-11-09 蔡巨才 Salvia miltiorrhiza cultivation medium and cultivation method

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CN104892231A (en) * 2015-06-14 2015-09-09 邵永存 Salvia miltiorrhiza seedling substrate and preparation method thereof
CN106866190A (en) * 2015-12-14 2017-06-20 中国中医科学院中药研究所 A kind of method of utilization Chinese medicine residue organic fertilizer cultivation Chinese medicine and its application

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108432592A (en) * 2018-04-19 2018-08-24 闽南师范大学 A kind of novel greening seedling matrix
CN109717038A (en) * 2019-03-13 2019-05-07 四川茂承生物科技有限公司 Radix Salviae Miltiorrhizae high quality seedling breeding method
CN113615539A (en) * 2020-05-07 2021-11-09 蔡巨才 Salvia miltiorrhiza cultivation medium and cultivation method
CN111631113A (en) * 2020-07-10 2020-09-08 南京中医药大学 Soilless seedling culture medium for salvia miltiorrhiza
CN112166844A (en) * 2020-11-11 2021-01-05 山东农业大学 Salvia miltiorrhiza seedling propagation method for vegetative propagation by using root segment pregermination

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Application publication date: 20171117