CN106866190A - A kind of method of utilization Chinese medicine residue organic fertilizer cultivation Chinese medicine and its application - Google Patents
A kind of method of utilization Chinese medicine residue organic fertilizer cultivation Chinese medicine and its application Download PDFInfo
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- CN106866190A CN106866190A CN201510694297.0A CN201510694297A CN106866190A CN 106866190 A CN106866190 A CN 106866190A CN 201510694297 A CN201510694297 A CN 201510694297A CN 106866190 A CN106866190 A CN 106866190A
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Abstract
The present invention relates to " method and its application using Chinese medicine residue organic fertilizer cultivation Chinese medicine ", the method that Chinese medicine slag is fermented into the method organic fertilizer using bacillus licheniformis DSKC-1, and the organic fertilizer is applied in Chinese medicine cultivation.The experiment in cultivation of the vegetables such as the Chinese medicines such as Radix Glycyrrhizae, the red sage root, Asian puccoon and cucumber is carried out according to disclosure of the invention method, as a result shows to be cultivated using Chinese medicine residue organic fertilizer, it is possible to increase above-mentioned Chinese medicine, the yield of vegetable crop cultivation.Chinese medicine slag is fermented into organic fertilizer by bacillus licheniformis KCDS-1 of the present invention, and be applied in cultivation production, yield can not only be improved, is a kind of organic cultivation method of new green environment protection while improving soil environment (such as nutrient, microecological balance, soil texture).
Description
Technical field
The invention belongs to bio-feritlizer production technical field, more particularly to a kind of utilization Chinese medicine residue organic fertilizer cultivates method and its application of Chinese medicine.
Background technology
It is long-term in Chinese medicine cultivation to ignore the use of organic fertilizer partially to fertilize, fertilizer efficiency decline is had resulted in, utilization rate is not high, the drawback such as soil hardening, continuous cropping obstacle are serious.And substantial amounts of research shows that using bio-organic fertilizer be the effective way that solves the above problems.The sustainable development of agricultural is extremely paid close attention in countries in the world, is increasing the exploitation of bio-feritlizer and organic fertilizer, is producing and apply dynamics.The amount of application of European some national bio-feritlizers has accounted for the 45%~60% of agricultural fertilizer total amount, and the amount of application in the U.S. is more up to 60% one 70%, and the annual production of China's bio-organic fertilizer now far can not meet the market demand less than 200,000 tons.
Chinese medicine slag is plant medicinal material remaining solid content after drug ingedient is extracted.China is natural resources of Chinese medicinal materials consumption big country, thereby produces substantial amounts of Chinese medicine dreg, and, up to 30,000,000 tons, up to 1,000,000 tons, the only big Chinese medicine pharmaceutical factory annual emissions in Nanjing six are up to more than 100,000 tons for such as annual emissions of Jiangsu Province's Chinese medicine slag for the annual emissions of national Chinese medicine slag in 2007；And it is 30,000 tons that Guizhou lark medicine company limited company in 2011 produces Chinese medicine slag per year；Two, Yunnan production notoginsenoside pharmaceutical factory just produces 150,000 tons of the dregs of a decoction every year.Chinese medicine slag rich in nutrition content, rich in there is the nutritional ingredients such as fiber, polysaccharide, albumen and trace element, light weight, aeration are good, are the organic fertilizer and Light media raw material of a kind of high-quality.But current waste residue of Chinese herbs majority is taken as domestic rubbish disposal, not only occupies substantial amounts of land resource, pollutes soil and underground water, and also result in the waste of resource.After Chinese medicine slag applies the raw-soil, the physicochemical character of the raw-soil can be made to improve significantly in the short period of time, be applied to during Chinese medicine is cultivated and have great importance, but the research of correlation is very few.
The content of the invention
Present invention aim at：(bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 16th in September in 2015 using bacillus licheniformis Bacillus licheniformis KCDS-1 to provide one kind, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number is CGMCC NO.11380) Chinese medicine slag is fermented into the method for organic fertilizer, and the organic fertilizer is applied in Chinese medicine cultivation.
Using the method for bacillus licheniformis KCDS-1 fermentation organic fertilizers, comprise the following steps：
(1) bacillus licheniformis is carried out into seed culture, fermentation tank culture, condition of culture is as follows：
Medium component is by percentage to the quality sucrose 0.3~0.7%, sodium chloride 0.3~0.7%, potassium dihydrogen phosphate 0.05~0.15%, yeast extract 1~3%, peptone 0.08~0.15%；
(2) bacillus licheniformis that will be cultivated is adsorbed using absorption carrier, and bacterium solution is 1: 1~2 with the weight ratio of absorption carrier, in the low temperature drying no more than 30 DEG C to 25~below 40%wt of water content after absorption, obtains microbial inoculum；
(3) by the microbial inoculum after step (2) and urea, fish meal proportionally for 10~12: 1: 3~4 mix, stirred (humidity of the dregs of a decoction is 45%~60%) according to 1: 3000~1: 5000 part by weight and Chinese medicine slag.Mix afterwards by dregs of a decoction heap coning, wide 1~2 meter, high 1~2 meter, it is impossible to less than 0.8 meter, plastic sheeting carries out heap fermentation in upper cover.Fermentation time 10~20 days, when dregs of a decoction raw material is overstrike or dark brown, the softness of holding is flexible when wet, very crisp when dry to be easily broken, and completes fermentation.The dregs of a decoction after fermentation, by drying, pulverization process, as practical Chinese medicine residue organic fertilizer.
The above-mentioned Chinese medicine residue organic fertilizer adaptable crop Chinese medicine such as including the red sage root, Radix Glycyrrhizae, purple perilla, it can also be used to the crops such as cucumber.
The application process of above-mentioned Chinese medicine residue organic fertilizer：Base manure can typically be made, topdressing uses, can be using spreading manuer in holes or the method such as strip ditch spread is carried out, then every mu of field amount of application is 100~200 kilograms.
The present invention is achieved through the following technical solutions：
The influence that the bighead atractylodes rhizome residue organic fertilizer of example 1 grows to cultivated licorice
(1) test material：Glycyrrhiza Seeds (Glycyrrhiza uralensis Fisch), bighead atractylodes rhizome residue organic fertilizer,
(2) test method：
1) seedling obtains：Using Seeds of Medicinal Plants through 75% ethanol sterilizing 1min, 10% hydrogen peroxide sterilization 10min, 30d is grown in the vermiculite disk that sterilizes；Or use tissue culturing seedling, sterile vermiculite disk hardening 30d.
2) seedling is transplanted, culture matrix is Chinese medicine residue organic fertilizer：Soil weight ratio is 1~2: 5, is managed according to normal management condition.
3) sampled after cultivating 6 months and be measured.
(3) experimental result
Result shows that bighead atractylodes rhizome residue organic fertilizer can promote the growth of Radix Glycyrrhizae aerial part and under ground portion.Bighead atractylodes rhizome residue organic fertilizer treatment group improves 1.81 times compared with space management group (without fertilising) for aerial part fresh weight；And 1.18 times are improve under ground portion fresh weight, concrete outcome is shown in accompanying drawing 1.
The influence that 23 kinds of Chinese medicine residue organic fertilizers of example grow to planted rooted salvia
(1) test material：Salvia seeds (Salvia miltiorrhiza Bge.), bighead atractylodes rhizome residue organic fertilizer, capsule of weeping forsythia residue organic fertilizer, malt residue organic fertilizer
(2) test method：
1) seedling obtains：Using Seeds of Medicinal Plants through 75% ethanol sterilizing 1min, 10% hydrogen peroxide sterilization 10min, 30d is grown in the vermiculite disk that sterilizes；Or use tissue culturing seedling, sterile vermiculite disk hardening 30d.
2) seedling is transplanted, culture matrix is Chinese medicine residue organic fertilizer：Soil weight ratio is 1~2: 5, is managed according to normal management condition.
3) sampled after cultivating 6 months and be measured.
(3) experimental result
Result shows that bighead atractylodes rhizome residue organic fertilizer, capsule of weeping forsythia residue organic fertilizer and malt residue organic fertilizer can promote red sage root ground, the growth of under ground portion.For promoting aerial part, it is compared with space management group (without fertilising), bighead atractylodes rhizome residue organic fertilizer treatment group improves 2.85 times, and capsule of weeping forsythia residue organic fertilizer treatment group improves 4.44 times, and malt residue organic fertilizer treatment group improves 6.33 times.For promoting under ground portion, it is compared with space management group (without fertilising), bighead atractylodes rhizome residue organic fertilizer treatment group improves 1.90 times, and capsule of weeping forsythia residue organic fertilizer treatment group improves 4.34 times, malt residue organic fertilizer treatment group improves 5.18 times, and concrete outcome is shown in accompanying drawing 2.
The influence of the red sage root residue organic fertilizer of example 3, grain to cultivation purple perilla growth
(1) test material：Purple perilla seed (Perilla frutescens (L.) Britt), bighead atractylodes rhizome residue organic fertilizer, capsule of weeping forsythia residue organic fertilizer, malt residue organic fertilizer
(2) test method：
1) seedling obtains：Using Seeds of Medicinal Plants through 75% ethanol sterilizing 1min, 10% hydrogen peroxide sterilization 10min, 30d is grown in the vermiculite disk that sterilizes；Or use tissue culturing seedling, sterile vermiculite disk hardening 30d.
2) seedling is transplanted, culture matrix is Chinese medicine residue organic fertilizer：Soil weight ratio is 1~2: 5, is managed according to normal management condition.
3) sampled after cultivating 6 months and be measured.
(3) experimental result
Result shows that red sage root residue organic fertilizer, malt residue organic fertilizer can promote purple perilla ground, the growth of under ground portion.For promoting aerial part, it is compared with space management group (without fertilising), bighead atractylodes rhizome residue organic fertilizer treatment group improves 2.85 times, and capsule of weeping forsythia residue organic fertilizer treatment group improves 4.44 times, and malt residue organic fertilizer treatment group improves 6.33 times.For promoting under ground portion, it is compared with space management group (without fertilising), bighead atractylodes rhizome residue organic fertilizer treatment group improves 1.90 times, and capsule of weeping forsythia residue organic fertilizer treatment group improves 4.34 times, malt residue organic fertilizer treatment group improves 5.18 times, and concrete outcome is shown in accompanying drawing 3.
Influence of the grain of example 4 to cucumber growth
(1) test material：Cucumber seeds (Cucumis sativus Linn), malt residue organic fertilizer
(2) test method：
1) seedling obtains：Using seed through 75% ethanol sterilizing 1min, 10% hydrogen peroxide sterilization 10min, 30d is grown in the vermiculite disk that sterilizes；Or use tissue culturing seedling, sterile vermiculite disk hardening 30d.
2) seedling is transplanted, culture matrix is Chinese medicine residue organic fertilizer：Soil weight ratio is 1~2: 5, is managed according to normal management condition.
3) sampled after cultivating 6 months and be measured.
(3) experimental result
Result shows that red sage root residue organic fertilizer, malt residue organic fertilizer can promote cucumber ground, the growth of under ground portion.For promoting to be compared with space management group (without fertilising), malt residue organic fertilizer treatment group improves 3.57 times for Aboveground Biomass of Young；79.8% is improved for plant height；2.29 times are improve for Underground biomass, concrete outcome is shown in accompanying drawing 4.
Brief description of the drawings
Fig. 1 is influence of the bighead atractylodes rhizome residue organic fertilizer to growth of Glycyrrhiza uralensis
Fig. 2 is influence of 3 kinds of Chinese medicine residue organic fertilizers to Salvia miltiorrhiza Growth
Fig. 3 is the influence that 3 kinds of Chinese medicine residue organic fertilizers grow to purple perilla
Fig. 4 is influence of the malt residue organic fertilizer to cucumber growth.
Claims (4)
1. a kind of method that application Chinese medicine residue organic fertilizer cultivates Chinese medicine, it is characterised in that include：It includes bacillus licheniformis
KCDS-1, absorption auxiliary material (wheat bran: rice chaff is 1: 1), urea and fish meal.First by bacillus licheniformis KCDS-1 zymotic fluids
Adsorbed according to 1: 1 ratio with absorption auxiliary material, dried；After after microbial inoculum drying by formula rate and urea, fish meal (microbial inoculum:
Urea: fish meal is to carry out mixture.
2. a kind of Chinese medicine slag preparation method of organic fertilizer based on described in claim 1, it is characterised in that comprise the following steps：
(1) bacillus licheniformis is carried out into seed culture, fermentation tank culture, condition of culture is as follows：
Medium component by percentage to the quality be sucrose 0.3~0.7%, sodium chloride 0.3~0.7%, potassium dihydrogen phosphate 0.05~0.15%,
Yeast extract 1~3%, peptone 0.08~0.15%；
(2) bacillus licheniformis that will be cultivated is adsorbed using absorption carrier, and bacterium solution is 1: 1~2 with the weight ratio of absorption carrier, is inhaled
The attached low temperature drying no more than 30 DEG C obtains microbial inoculum to 25~below 40%wt of water content；
(3) by the microbial inoculum after step (2) and urea, fish meal proportionally for 10~12: 1: 3~4 mix, according to 1: 3000~1: 5000
Part by weight and Chinese medicine slag stir (humidity of the dregs of a decoction is 45%~60%).Mix afterwards by dregs of a decoction heap coning, it is wide by 1~2
Rice, high 1~2 meter, it is impossible to less than 0.8 meter, plastic sheeting carries out heap fermentation in upper cover.Fermentation time 10~20 days, when
Dregs of a decoction raw material is overstrike or dark brown, and the softness of holding is flexible when wet, very crisp when dry to be easily broken, and completes fermentation.
The dregs of a decoction after fermentation, by drying, pulverization process, as practical Chinese medicine residue organic fertilizer.
3. it is a kind of to be applied in Chinese medicines such as Radix Glycyrrhizae, the red sage root, the Radix Astragali, purple perillas based on claim (1) and (2) Chinese medicine residue organic fertilizer
In cultivation, in can also be applied to the vegetable growings such as cucumber.
4. it is a kind of to be based on claim (1), the application process of (2) and (3) Chinese medicine residue organic fertilizer：Base manure can typically be made, topdressing makes
With, can be using spreading manuer in holes or the method such as strip ditch spread is carried out, then every mu of field amount of application is 100~200 kilograms.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347611A (en) * | 2017-08-30 | 2017-11-17 | 中国医学科学院药用植物研究所海南分所 | A kind of red sage root soilless culture method |
| CN108432592A (en) * | 2018-04-19 | 2018-08-24 | 闽南师范大学 | A kind of novel greening seedling matrix |
| CN108476652A (en) * | 2018-02-09 | 2018-09-04 | 广西梧州制药（集团）股份有限公司 | A method of promote picria fel tarrae seed to sprout using Chinese medicine slag |
| CN110304955A (en) * | 2019-03-12 | 2019-10-08 | 康定恩威高原药材野生抚育基地有限责任公司 | A kind of Chinese medicine dreg organic fertilizer, preparation method and modification method for improveing bulbus fritillariae cirrhosae planting soil |
| WO2020207305A1 (en) * | 2019-04-11 | 2020-10-15 | 湖南诺泽生物科技有限公司 | Method for interplanting arbor-type traditional chinese medicine materials, shrub-type traditional chinese medicine materials and herb-type traditional chinese medicine materials |
Citations (2)
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| US20040172997A1 (en) * | 2003-03-06 | 2004-09-09 | Yuen Foong Yu Paper Mfg Co. Ltd. | Plant nutrition formulated by recovery filtrate from plant fiber biopulp and method thereof |
| CN104560816A (en) * | 2014-12-16 | 2015-04-29 | 大地绿源环保科技(北京)有限公司 | Bacillus licheniformis with biomass hydrolase activity and application thereof |
-
2015
- 2015-12-14 CN CN201510694297.0A patent/CN106866190A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040172997A1 (en) * | 2003-03-06 | 2004-09-09 | Yuen Foong Yu Paper Mfg Co. Ltd. | Plant nutrition formulated by recovery filtrate from plant fiber biopulp and method thereof |
| CN104560816A (en) * | 2014-12-16 | 2015-04-29 | 大地绿源环保科技(北京)有限公司 | Bacillus licheniformis with biomass hydrolase activity and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347611A (en) * | 2017-08-30 | 2017-11-17 | 中国医学科学院药用植物研究所海南分所 | A kind of red sage root soilless culture method |
| CN108476652A (en) * | 2018-02-09 | 2018-09-04 | 广西梧州制药（集团）股份有限公司 | A method of promote picria fel tarrae seed to sprout using Chinese medicine slag |
| CN108432592A (en) * | 2018-04-19 | 2018-08-24 | 闽南师范大学 | A kind of novel greening seedling matrix |
| CN110304955A (en) * | 2019-03-12 | 2019-10-08 | 康定恩威高原药材野生抚育基地有限责任公司 | A kind of Chinese medicine dreg organic fertilizer, preparation method and modification method for improveing bulbus fritillariae cirrhosae planting soil |
| WO2020207305A1 (en) * | 2019-04-11 | 2020-10-15 | 湖南诺泽生物科技有限公司 | Method for interplanting arbor-type traditional chinese medicine materials, shrub-type traditional chinese medicine materials and herb-type traditional chinese medicine materials |
| CN114025604A (en) * | 2019-04-11 | 2022-02-08 | 湖南诺泽生物科技有限公司 | Interplanting method for arbor, shrub and herb traditional Chinese medicinal materials |
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