CN103011918A - Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight - Google Patents
Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight Download PDFInfo
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- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 65
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 61
- 230000000813 microbial effect Effects 0.000 title abstract description 8
- 239000002686 phosphate fertilizer Substances 0.000 title abstract 5
- 239000003337 fertilizer Substances 0.000 claims abstract description 55
- 238000000855 fermentation Methods 0.000 claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 22
- 230000000443 biocontrol Effects 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000003042 antagnostic effect Effects 0.000 claims abstract description 8
- 239000004021 humic acid Substances 0.000 claims abstract description 7
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- 241000894006 Bacteria Species 0.000 claims abstract description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 4
- 239000002367 phosphate rock Substances 0.000 claims abstract description 4
- 244000005700 microbiome Species 0.000 claims description 34
- 230000001580 bacterial effect Effects 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 8
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- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 6
- 108010046845 tryptones Proteins 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
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- 235000019219 chocolate Nutrition 0.000 claims description 3
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- 238000009335 monocropping Methods 0.000 abstract description 17
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- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 244000025254 Cannabis sativa Species 0.000 abstract 1
- 240000003826 Eichhornia crassipes Species 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 231100000956 nontoxicity Toxicity 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 8
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000908271 Ilyonectria destructans Species 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 240000000851 Vaccinium corymbosum Species 0.000 description 2
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- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241000223602 Alternaria alternata Species 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Fertilizers (AREA)
Abstract
The invention relates to a microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight, belonging to the technical field of microbial organic phosphate fertilizers. The microbial organic phosphate fertilizer is prepared by the steps of preparing biocontrol bacterium seed solution through liquid fermentation cultivation by using bacillus amyloliquefaciens; mixing and fermenting crushed water hyacinth residue, grass peat, humic acid and powdered rock phosphate with content of 20-24% and fineness of 400-450meshes according to the proportion of 5:3:1:1 to obtain biological phosphatic fertilizer matrix; and uniformly mixing the biocontrol bacterium seed solution and the biological phosphatic fertilizer matrix to prepare the microbial organic phosphate fertilizer capable of preventing and controlling the pseudo-ginseng blight, wherein the fertilizer contains antagonistic microbes being more than 1*1010 per unit gram, 5-6% of total nitrogen content, 6-8% of total nitrogen phosphorus and potassium and 40% of organic matters. The microbial organic phosphate fertilizer has the advantages of low cost, convenience in use, remarkable effect, and no toxicity, harmlessness and no environmental pollution, can thoroughly sterilize harmful germs in pseudo-ginseng soil, supplements nutrient elements required by growth of pseudo-ginseng plants, and improves the physical and chemical properties and microbial activity of soil with continuous cropping obstacles and stress resistance of the pseudo-ginseng plants.
Description
Technical field:
The present invention relates to a kind of microorganism organic phosphatic fertiliser of preventing and treating the pseudo-ginseng blight, microorganism belonging to genus organic phosphatic fertiliser technical field.
Background technology:
Pseudo-ginseng (Panax notoginseng (Burk.) F.H.Chen) is the araliaceae ginseng plant, is the distinctive rare traditional Chinese medicine of China [1], also is one of most characteristic Biological resources in Yunnan Province.Pseudo-ginseng has many-sided pharmacological action and the nourishing functions [2-3] such as loose stasis of blood hemostasis, subduing swelling and relieving pain, adjusting blood glucose balance, antifatigue, anti-ageing, hypoxia tolerance and raising immunity of organisms.Pseudo-ginseng is the perennial root herbaceous plant, because the Panax notoginseng Growth cycle is longer, growth conditions is harsh (to be required cool in summer and warm in winter, without severe cold and heat, half light half is cloudy, moist specific environment), planting area is limited, only be distributed in several places [4] that each county, mountain of papers city, Yunnan and Guangxi and mountain of papers have a common boundary, therefore with the congener ginseng, Radix Panacis Quinquefolii is the same, there is serious Soil-sickness Problem [5], the soil that namely planted one batch of pseudo-ginseng takes interval at least eight years even could plant pseudo-ginseng decades again, if shorten the crop rotation time limit then to show as the pseudo-ginseng aerial growth bad, disease is many, yield poorly, even ruin garden total crop failure [6].Pseudo-ginseng blight (Root rot disease) is the destructive disease during pseudo-ginseng produces, and its loss that causes accounts for the 70-85% of the whole disease losses of pseudo-ginseng, has become the serious hindrance of restriction pseudo-ginseng industry Sustainable development.To be that multiple pathogenic bacteria is collaborative infect the soil that causes and pass compound disease (Complex disease) the pseudo-ginseng blight, its cause of disease comprises fungi (Alternaria panax, A. tenuis, Cylindrocarpon destructans, C. didynum, Fusarium solani, F. oxysporum, Phytophthora cactorum, Phoma herbarum, Rhizoctonia solani) [7].Add up according to investigations: pseudo-ginseng crop rotation interval 4～5 years, sickness rate reaches more than 30%; The interval is more than 8 years, and sickness rate is at 10%～15%[7].Therefore, continuous cropping obstacle causes pseudo-ginseng suitable planting plot very nervous, has presented by the suitable planting zone and has shifted to inferior Production Zones Suitable, has not only increased the planting cost of " seven farmings ", has also had a strong impact on output and the quality of pseudo-ginseng.Continuous cropping obstacle also becomes the significant problem of restriction pseudo-ginseng industry development.Utilize to open up wasteland newly and with non-host crop crop rotation or to lie fallow more than 8～10 years be that tradition addresses this problem effective means.Along with the continuous expansion of pseudo-ginseng large-scale planting area, this method can not need by satisfying the market, directly affects pseudo-ginseng quality and contaminate environment and use chemical pesticide but in recent years.Therefore, a kind of safe and effective prophylactico-therapeutic measures, under this background, biological control is endowed great expectation.
Pseudo-ginseng is as a kind of regional plant, and the biological control of the compound disease of its root-rot is seldom by extensive concern, preferably application prospect arranged in the pseudo-ginseng blight aspect preventing and treating but part Study has shown Biocontrol microorganism.Process with microbiobacterial agent seed dressing (seedling), at Panax notoginseng Growth in earlier stage, blight is had obvious control effect (Chen Yujun etc., 2004); Three bacillus that separate from the pseudo-ginseng rhizosphere soil have preferably bacteriostatic action to the corrupted post spore in the pseudo-ginseng Pathogen of Fusarium Wilt mould (C. destructans), possess the potentiality (Liu Lizhi etc., 2004) of research and development biological pesticide.Biocontrol microorganism is grown in good the deciding of plant rhizosphere, existence and fecundity are prerequisite of its performance biocontrol effect; Good rhizospere competition has become the important investigation parameter of screening soil-borne disease antagonistic microbe.Biological organic phosphatic fertiliser is that the phosphorus ore thing of employing process high energy activation is main raw material, obtains with organic co-fermentation processing under the effect of special efficacy bacterial classification; Be characterized in long-acting slow-release, non-acidizing, water-insoluble, aftereffect is remarkable, uses many seasons effective in a season; This fertilizer make using energy soil mineral the Nomenclature Composition and Structure of Complexes, avoid chemical fertilizer to the detrimentally affect of agricultural product quality and soil acidification, reach volume increase, improve quality, the effect of green ecological.
The present invention utilizes a kind of efficient Antagonistic Fungi (bacillus amyloliquefaciens) that can efficiently eliminate pseudo-ginseng blight continuous cropping obstacle to combine with biological organic phosphatic fertiliser, produces a kind ofly can carry out the fertile double effect biological organic fertilizer material of medicine that biological antiseptic can provide for planting site again lasting nutrient to the soil soil-borne disease of plantation pseudo-ginseng.By literature search, have no the open report identical with the present invention.
Reference:
[1] Chinese Pharmacopoeia Commission. Pharmacopoeia of People's Republic of China (version in 2010). Beijing: Chemical Industry Press, 2010:10-11.
[2]?Sun?S,?Wang?CZ,?Tong?R,?Li?XL,?Fishbein?A,?Wang?Q,?He?TC,?Du?W,?Yuan?CS.?Effects?of?steaming?the?root?of?Panax?notoginseng?on?chemical?composition?and?anticancer?activities.?Food?Chemistry,?2010,?118(2):?307-314.
[3] good rain after a long drought, Xu Huibo, Sun Xiaobo. the Advance on Pharmacological Activities of Radix Notoginseng total arasaponins. the time precious traditional Chinese medical science traditional Chinese medicines, 2007,18 (5): 1251-1252.
[4]?Guo?HB,?Cui?XM,?An?N?and?Cai?GP.?Sanchi?ginseng?(Panax?notoginseng?(Burkill)?F.?H.?Chen)?in?China:distribution,?cultivation?and?variations.?Genetic?Resources?and?Crop?Evolution,?2010,?57:?453-460.
[5] simple the friend, Wang Wenquan, Meng Li, Zhang Zilong. Panax medicinal plant continuous cropping obstacle progress. contemporary Chinese Chinese medicine, 2008,10 (6): 3-5.
[6] Zhang Chongyi, Lin Wenxiong. the allelopathic of medicinal plant is from toxic action and continuous cropping obstacle. Chinese Ecological Agriculture journal, 2009,17 (1): 189-196.
[7] Miu Zuoqing, Li Shidong, Liu Xingzhong, etc. the research of notoginseng root rot cause of disease. Scientia Agricultura Sinica. 2006,39:1371-1378.
[8] Chen Yujun, Wang Yong, Liu Yunzhi, etc. the preliminary study of bacteria agent control notoginseng root rot. CHINA JOURNAL OF CHINESE MATERIA MEDICA. 2004,29 (11): 1102-1103.
[9] Liu Lizhi, Wang Qifang, Zhang Keqin, etc. the initial analysis of notoginseng root rot antagonism screening and active result. the journal .2004 of Yunnan University, 6 (4): 357-359.
Summary of the invention:
The object of the invention is to overcome the deficiency of prior art, and provide a kind of easy to use, cost is low, successful, the microorganism organic phosphatic fertiliser of nontoxic, harmless control pseudo-ginseng blight.
The object of the present invention is achieved like this:
The present invention separates from the blueberry growing area blueberry rhizosphere soil of Qujing City of Yunnan Province Fuyuan County and obtains bacterial strain KMXY1, its qualification result is shown that this bacterium belongs to bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and this bacterial strain has been kept at China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: China. No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: September 21 in 2012; Preserving number: CGMCC NO. 6598.This bacillus amyloliquefaciens thalline feature and colony characteristics: thalline is rod-short, even dyeing, and the tool mobility, amphimicrobian can form endogenous spore, and sporangium is expanded, ovalize, a little less than free spore surface is painted, Gram-positive.At the LB substratum opaque colony that is white in color, surface irregularity, colony edge is irregular, does not all produce pigment on multiple substratum.This Antagonistic Fungi and organic phosphatic fertiliser compost are mixed and made into microorganism organic fertilizer, contain 1 * 10 in the fertilizer
10The antagonistic microbe quantity that individual/g is above, total nitrogen phosphorus potassium nutrition is 6～8%, organic content is 40%.
The microorganism organic phosphatic fertiliser of control pseudo-ginseng blight of the present invention obtains through following concrete steps:
(1), the preparation of biocontrol microorganisms seed liquor
A. be that the KMXY1 bacterial strain of CGMCC NO. 6598 is transplanted to the test tube that contains the LB substratum and is carried out a ring activation with preserving number, cultivated 48 hours for 29 ℃, the LB culture medium prescription is: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, pH7.0, agar 17g/L;
B. get lawn after the ring activation move into the bottled 600ml of 1000ml taper by Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, in the LB nutrient solution that pH7.0 forms, turn 110/min, cultivated 72 hours for 29 ℃ times, obtain zymocyte liquid;
C. will train zymocyte liquid and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the seeding tank in the ratio that inoculum size 1:200 trains, 29-31 ℃ fermented 16-18 hour, obtained the fermentation bacterial classification;
D. will ferment and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the fermentor tank with bacterial classification in inoculum size 1:20 ratio doubly, 29-31 ℃ of fermentation is after 72-96 hour, when gemma content was more than or equal to 85-90% in the fermented liquid in the fermentor tank, namely obtaining bacterial content was 1 * 10
11During the above bacterium liquid of individual/g, go out tank stores as the biocontrol microorganisms seed liquor;
(2), the preparation of biological organic phosphatic fertiliser matrix
A. the respectively Herba Eichhorniae slag is dry and pulverizing, smashing fineness≤5cm; Peat composed of rotten mosses oven dry or air-dry rear pulverizing are crushed to≤0.45mm; Humic acid powder is broken to≤0.45mm; Content 20-24%, ground phosphate rock are crushed to≤400 orders;
B. after a. step being pulverized Herba Eichhorniae slag, the peat composed of rotten mosses, humic acids, phosphorus ore opaque in mass ratio the ratio of 5:3:1:1 mix, heap was sealed rear spontaneous fermentation 20-25 days, when rising to 62 ℃, temperature carries out turning therebetween with abundant fermentation, continue fermentation after the turning, until material is when being grey chocolate, the material fermenting-ripening namely gets biological organic phosphatic fertiliser matrix;
(3), the preparation of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
Be 1 * 10 with the biocontrol microorganisms seed liquor bacterial content of (1) step
11Individual/when g is above and the biological organic phosphatic fertiliser matrix of step (2) mix, make and contain 1 * 10 in the mixed fertilizer
10Above antagonistic microbe quantity, the total nitrogen content of individual/g is 5～6%, and total nitrogen phosphorus potassium nutrition is 6～8%, organic content is 40%, and packing is finished product.
The used equipment of the present invention is the general-purpose equipment of market sale.
The invention has the advantages that:
1, cost is low, and is easy to use, and successful is nontoxic, harmless, pollute without environment.
2, this fertilizer can not only carry out sufficiently sterilised to the harmful germ in the plantation pseudo-ginseng soil, and can replenish the required nutritive element of Panax notoginseng Growth, and physicochemical character and the soil microbial activities of improvement continuous cropping obstacle soil improve the resistance of pseudo-ginseng plant.
Embodiment:
The used equipment of embodiment is the general-purpose equipment of market sale.
Embodiment 1:
1, the microorganism organic phosphatic fertiliser of preparation control pseudo-ginseng blight
(1), the preparation of biocontrol microorganisms seed liquor
A. be that the KMXY1 bacterial strain of CGMCC NO. 6598 is transplanted to the test tube that contains the LB substratum and is carried out a ring activation with preserving number, cultivated 48 hours for 29 ℃, the LB culture medium prescription is: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, pH7.0, agar 17g/L;
B. get lawn after the ring activation move into the bottled 600ml of 1000ml taper by Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, in the LB nutrient solution that pH7.0 forms, turn 110/min, cultivated 72 hours for 29 ℃ times, obtain zymocyte liquid;
C. will train zymocyte liquid and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the seeding tank in the ratio that inoculum size 1:200 trains, 29 ℃ or 30 ℃ or 31 ℃ fermented 16 hours or 17 hours or 18 hours, and obtained the fermentation bacterial classification;
D. will ferment and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the fermentor tank with bacterial classification in inoculum size 1:20 ratio doubly, or 30 ℃ or 31 ℃ fermentation is after 72 hours or 86 hours or 96 hours, when gemma content in the fermented liquid in the fermentor tank more than or equal to 85 or 90% the time, go out tank stores as the biocontrol microorganisms seed liquor;
(2), the preparation of biological organic phosphatic fertiliser matrix
A. the respectively Herba Eichhorniae slag is dry and pulverizing, smashing fineness≤5cm; Peat composed of rotten mosses oven dry or air-dry rear pulverizing are crushed to≤0.45mm; Humic acid powder is broken to≤0.45mm; Content 20% or 24% ground phosphate rock are crushed to≤400 orders;
B. after a. step being pulverized Herba Eichhorniae slag, the peat composed of rotten mosses, humic acids, phosphorus ore opaque in mass ratio the ratio of 5:3:1:1 mix, heap was sealed rear spontaneous fermentation 20-25 days, when rising to 62 ℃, temperature carries out turning therebetween with abundant fermentation, continue fermentation after the turning, until material is when being grey chocolate, the material fermenting-ripening namely gets biological organic phosphatic fertiliser matrix;
(3), the preparation of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
Be 1 * 10 with the biocontrol microorganisms seed liquor bacterial content of (1) step
11Individual/when g is above and the biological organic phosphatic fertiliser matrix of step (2) mix, make and contain 1 * 10 in the mixed fertilizer
10Above antagonistic microbe quantity, the total nitrogen content of individual/g is 5% or 6%, and total nitrogen phosphorus potassium nutrition is 6% or 7% or 8%, organic content is 40%, and packing is finished product.
2, the application of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
2009-2011 carries out correlation test in Yunnan Province Wenshan Prefecture, and is basic identical for examination agricultural land soil physicochemical character, middle fertility.
Adopt Random Design, establish continuous cropping soil, this Term Fertilization continuous cropping soil and newly (not yet planted pseudo-ginseng) 3 processing, every processing repeats 3 times, and each residential quarter area is 200m
2In November, 2009, do to being about to use this fertilizer embodiment that base fertilizer is disposable to be manured into soil, consumption is 16000kg/hm
2, then evenly turn over; Continuous cropping and new work ground are processed and are used composite fertilizer 400kg/hm according to local ordinary method
2, on January 15th, 2010 was transplanted annual pseudo-ginseng seedling plant, and planting density is 350,000/hm
2, all the other field management measures are with local conventional.Carry out field investigation in December, 2010, the situations such as the growth of investigation pseudo-ginseng and disease generation, results are surveyed and are produced.Experimental result such as following table.
Anti-blight microorganism organic phosphatic fertiliser is to the field test results of pseudo-ginseng wilt
Embodiment 2:
1, the microorganism organic phosphatic fertiliser of preparation control pseudo-ginseng blight
The preparation process of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight is with embodiment 1.
2, the application of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
2009-2011 carries out correlation test in Yunnan Province Wenshan Prefecture, and is basic identical for examination agricultural land soil physicochemical character, middle fertility.
Adopt Random Design, establish continuous cropping soil, this Term Fertilization continuous cropping soil and newly (not yet planted pseudo-ginseng) 3 processing, every processing repeats 3 times, residential quarter area 200m
2In November, 2009, do to being about to use this fertilizer embodiment that base fertilizer is disposable to be manured into soil, consumption is 20000kg/hm
2, then evenly turn over; Continuous cropping and new work ground are processed and are used composite fertilizer 400kg/hm according to local ordinary method
2, on January 15th, 2010 was transplanted annual pseudo-ginseng seedling plant, and planting density is 350,000/hm
2, all the other field management measures are with local conventional.Carry out field investigation in December, 2010, the situations such as the growth of investigation pseudo-ginseng and disease generation, results are surveyed and are produced.Experimental result such as following table:
Anti-blight microorganism organic phosphatic fertiliser is to the field sick soil test-results of pseudo-ginseng wilt
Embodiment 3:
1, the microorganism organic phosphatic fertiliser of preparation control pseudo-ginseng blight
The preparation process of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight is with embodiment 1.
2, the application of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
2009-2011 carries out correlation test in Yunnan Province Wenshan Prefecture, and is basic identical for examination agricultural land soil physicochemical character, middle fertility.Adopt Random Design, establish continuous cropping soil, this Term Fertilization continuous cropping soil and newly (not yet planted pseudo-ginseng)
3 processing, every processing repeat 3 times, residential quarter area 200m
2In November, 2009, do to being about to use this fertilizer embodiment that base fertilizer is disposable to be manured into soil, consumption is 25000kg/hm
2, then evenly turn over; Continuous cropping and new work ground are processed and are used composite fertilizer 400kg/hm according to local ordinary method
2, on January 15th, 2010 was transplanted annual pseudo-ginseng seedling plant, and planting density is 350,000/hm
2, all the other field management measures are with local conventional.Carry out field investigation in December, 2010, the situations such as the growth of investigation pseudo-ginseng and disease generation, results are surveyed and are produced.Experimental result such as following table:
Anti-blight microorganism organic phosphatic fertiliser is to the field sick soil test-results of pseudo-ginseng wilt
The test-results of above-mentioned 3 routine embodiment all shows, the microorganism organic phosphatic fertiliser finished product of control pseudo-ginseng soil-borne disease of the present invention is for the stronger promoter action of growing of continuous cropping soil pseudo-ginseng, make thick and single the weight average of plant height, the stem of pseudo-ginseng that increase be arranged, and better for the prevention effect of pseudo-ginseng disease, obviously reduced the sickness rate of pseudo-ginseng blight.The pseudo-ginseng of in Wenshan Prefecture's continuous cropping soil, planting through fertilizer finished product of the present invention, its growing way growing way basic and new pseudo-ginseng on the ground is close, and its economic yield also has a larger increase, economic benefit and obvious environment benefit, show thus, use the net effect of this invention fertilizer finished product good.
Claims (1)
1. microorganism organic phosphatic fertiliser of preventing and treating the pseudo-ginseng blight is characterized in that this microorganism organic phosphatic fertiliser is through following steps system:
(1), the preparation of biocontrol microorganisms seed liquor
A. be that the KMXY1 bacterial strain of CGMCC NO. 6598 is transplanted to the test tube that contains the LB substratum and is carried out a ring activation with preserving number, cultivated 48 hours for 29 ℃, the LB culture medium prescription is: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, pH7.0, agar 17g/L;
B. get lawn after the ring activation move into the bottled 600ml of 1000ml taper by Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, in the LB nutrient solution that pH7.0 forms, turn 110/min, cultivated 72 hours for 29 ℃ times, obtain zymocyte liquid;
C. will train zymocyte liquid and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the seeding tank in the ratio that inoculum size 1:200 trains, 29-31 ℃ fermented 16-18 hour, obtained the fermentation bacterial classification;
D. will ferment and be inoculated in fermentation culture in the LB nutrient solution identical with the b step in the fermentor tank with bacterial classification in inoculum size 1:20 ratio doubly, 29-31 ℃ of fermentation is after 72-96 hour, when gemma content was more than or equal to 85-90% in the fermented liquid in the fermentor tank, namely obtaining bacterial content was 1 * 10
11During the above bacterium liquid of individual/g, go out tank stores as the biocontrol microorganisms seed liquor;
(2), the preparation of biological organic phosphatic fertiliser matrix
A. the respectively Herba Eichhorniae slag is dry and pulverizing, smashing fineness≤5cm; Peat composed of rotten mosses oven dry or air-dry rear pulverizing are crushed to≤0.45mm; Humic acid powder is broken to≤0.45mm; Content 20-24%, ground phosphate rock are crushed to≤400 orders;
B. after a. step being pulverized Herba Eichhorniae slag, the peat composed of rotten mosses, humic acids, phosphorus ore opaque in mass ratio the ratio of 5:3:1:1 mix, heap was sealed rear spontaneous fermentation 20-25 days, when rising to 62 ℃, temperature carries out turning therebetween with abundant fermentation, continue fermentation after the turning, until material is when being grey chocolate, the material fermenting-ripening namely gets biological organic phosphatic fertiliser matrix;
(3), the preparation of the microorganism organic phosphatic fertiliser of control pseudo-ginseng blight
Be 1 * 10 with the biocontrol microorganisms seed liquor bacterial content of (1) step
11Individual/when g is above and the biological organic phosphatic fertiliser matrix of step (2) mix, make and contain 1 * 10 in the mixed fertilizer
10Above antagonistic microbe quantity, the total nitrogen content of individual/g is 5～6%, and total nitrogen phosphorus potassium nutrition is 6～8%, organic content is 40%, and packing is finished product.
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| CN105085101A (en) * | 2014-05-19 | 2015-11-25 | 杨耀军 | Preparation method of bacillus amyloliquefaciens mineral fertilizer |
| CN105103854A (en) * | 2015-08-11 | 2015-12-02 | 昆明杏林大观园旅游开发有限公司 | Stereoscopic cultivation facility and technology for panax notoginseng |
| CN108034589A (en) * | 2018-01-11 | 2018-05-15 | 云南农业大学 | A kind of method for screening pseudo-ginseng rhizosphere biocontrol microorganisms microorganism |
| CN108887122A (en) * | 2018-07-27 | 2018-11-27 | 上海环垦生态科技股份有限公司 | Notoginseng planting method |
| CN110776352A (en) * | 2019-11-22 | 2020-02-11 | 云南省烟草公司临沧市公司 | Functional biological organic fertilizer for tobacco planting soil |
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