CN107333571A - A kind of fruit of a cubeb litsea tree engrafting method - Google Patents
A kind of fruit of a cubeb litsea tree engrafting method Download PDFInfo
- Publication number
- CN107333571A CN107333571A CN201710710914.0A CN201710710914A CN107333571A CN 107333571 A CN107333571 A CN 107333571A CN 201710710914 A CN201710710914 A CN 201710710914A CN 107333571 A CN107333571 A CN 107333571A
- Authority
- CN
- China
- Prior art keywords
- fruit
- scion
- grafting
- cubeb litsea
- litsea tree
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/04—Grafting-wax
Abstract
The invention belongs to planting technology field, specifically provides a kind of fruit of a cubeb litsea tree engrafting method, and it is selection root coppice shoot or cuts after bar the side shoot for sprouting out as scion, and grafting is carried out with cut-grafting method.The fruit of a cubeb litsea tree engrafting method of the present invention overcome require sterile working existing for fruit of a cubeb litsea tree tissue culture propagation in the prior art, the problem of reproductive-cost is high, there is provided easy, low cost, the fruit of a cubeb litsea tree propagation method suitable for field production.Scion acquisition method used in the present invention may insure the vitality of scion, be greatly enhanced the success rate of grafting；The present invention improves more than 20% using cut-grafting method than other grafting method survival rates.
Description
Technical field
The invention belongs to planting technology field, and in particular to plant asexual reproduction method, more particularly it relates to one
Kind fruit of a cubeb litsea tree engrafting method.
Background technology
Fruit of a cubeb litsea tree tool has been widely used, and pericarp extraction litsea cubeba oil, benevolence extraction fatty oil, and Chinese herbal medicine are used
Deng.Fruit of a cubeb litsea tree complete stool contains essential oil, pericarp essential oil content general about 3%~4%.Litsea cubeba oil contains citral, higher alcohol, had
Machine acid, wherein main component lemon aldehyde is up to 60%~80%, reaches as high as 90%, is manufacture ionone series spices
Important source material, the serial spices is widely used in the daily chemical products such as the cosmetics of super quality, perfumed soap.Refined litsea citrate oil is good
Good foodstuff flavouring agent and preservative, can be directly used for the seasoning flavouring of confection, beverage, flavouring etc..Litsea citrate oil is also
Can be as the raw material of synthesising complex E, K, A etc..Oil-containing 30~40% or so in litsea cubeba kernel benevolence, may separate out a variety of oleic acid
Product, such as laurate, capric acid, dodecenoic acid, oleic acid, these compositions are the primary raw materials of daily-use chemical industry.Fruit of a cubeb litsea tree pomace
It can be used for manufacturing feed and feed natural fungicidal agent.Litsea cubeba root, stem, leaf and fruit can be used as medicine, and have expelling wind and clearing away cold, qi-regulating
The effect of analgesic, its fruit of Chinese medicine are referred to as " cubeb ".Generally speaking, the main economic development of the fruit of a cubeb litsea tree is food at present
With chemical product litsea cubeba oil.
Litsea citrate oil is one of major product of China's essence and flavoring agent industry.China's essence and flavoring agent industry develops fast in recent years
Suddenly.China's litsea citrate oil output and export volume rank first in the world for a long time, and export amount is also more stable.For many years I
State exports state's litsea citrate oil such as American-European-Japanese and is basically stable at 2500-3000 tons.Annual production increases in recent years, and about more than 4000
Ton, annual export volume reach 3500 tons or so.On Yunnan, Guizhou, Chongqing and other places, litsea cubeba oil, fruit of a cubeb litsea tree fresh leaf, fresh fruit all may be used
Directly used as food condiments.Due to the shortage of natural lemon aldehyde raw material, supply falls short of demand for the outlet of litsea cubeba oil.But mountain
Grey sub- industry accounts for 80% at present mainly by wild standing forest.
Artificial forest construction lacks breeding, because fruit of a cubeb litsea tree dioecism, it is necessary to strengthen the fast numerous work of fruit of a cubeb litsea tree breeding, with
Solves the active demand to breeding in production.As dioecious plant, to cultivate the stable fruit of a cubeb litsea tree of character, its is asexual numerous
Plantation technology is extremely important.The fruit of a cubeb litsea tree is because complete stool is rich in terpene substances so that cuttage faces the problem of rooting rate, and grafting, which faces, to be connect
The problem of fringe and stock affinity.
By retrieval, following two patent documents are found：A kind of fruit of a cubeb litsea tree Clonal regeneration method for culturing seedlings
(CN201110088322) it is, with fruit of a cubeb litsea tree tissue culture subculture bud Rooting ex vitro, with fruit of a cubeb litsea tree choiceness tissue culture subculture bud
For cuttings, the method for the outer nursery of the excellent strain tissue culture subculture bud test tube of the fruit of a cubeb litsea tree or tissue-cultured seedling juvenile form branch cuttage and seedling culture.The inventive method
Filial generation can be made to keep the excellent hereditary capacity of maternal plant, and solve the problems, such as fruit of a cubeb litsea tree breeding asxualization nursery.Other one, induction
The method (CN200710170434) of litsea cubeta multiple shoot, the brown of material is controlled using the method for Transition medium and light culture
Change；The bud inducement cultivation stage adds GA, and the Multiplying culture stage selects the method that proliferated culture medium and inducing culture are used alternatingly,
Bud sprout time shifts to an earlier date, and quickly enters vegetative state, and obtained sprout is healthy and strong neat.The invention can form a large amount of excellent in a short time
Good test tube seedling, carry out large-scale production.Although patent document disclosed above solved the problems, such as with present patent application it is essentially identical,
Technical scheme differs, and the grafting of the application is fast numerous independent of sterile working, suitable for field production practices.
The content of the invention
It is an object of the invention to overcome to require sterile working existing for fruit of a cubeb litsea tree tissue culture propagation in the prior art, be multiplied into
The problem of this is high, there is provided easy, low cost, the fruit of a cubeb litsea tree engrafting method suitable for field production.
The present invention provides a kind of fruit of a cubeb litsea tree engrafting method, and it makees for the side shoot for sprouting out after selection root coppice shoot or section bar
For scion, grafting is carried out with cut-grafting method.
The present invention provides a kind of fruit of a cubeb litsea tree engrafting method, comprises the following steps：
A gathers root coppice shoot, or cuts after bar the side shoot for sprouting out as scion, it is desirable to scion semi-lignified, diameter
For 3-5 millimeters, have some full buds；
The trunk that B leaves after cutting bar using biennial fruit of a cubeb litsea tree tree is stock；
C is in the lunar calendar first month of the lunar year to the beginning of February with cut-grafting method grafting.
Wherein, described in A collection gathered before leaf bud bud is sprouted, i.e., in the winter time (lunar calendar November to December) collection or
Spring (lunar calendar first month of the lunar year) gathers.
The winter collection, scion low-temperature moisture preservation are preserved to next spring grafting.The scion low-temperature moisture preservation preserves, scion
Bottom sealing wax, 0-10 DEG C of preservation, is buried with wet sand, and husky humidity is pinched as group with hand, and hand pine is spread preferably, and the depth of burial is to connect
2/3rds of fringe height.The next spring grafting, scion wax-sealing part is cut off 2-4 centimetres before grafting, and scion is soaked
Enter diel in water, allow scion fully to absorb water.
The spring collection, scion moisturizing, same day grafting.The scion moisturizing is to bind up with gauze base segment with wet, is removed
Blade moisturizing.
Wherein, stock described in B, 50-60 centimetres of height is retained after cutting strunk.
The selection at stock age is important, biennial to be advisable, too high less than 2 years or the age of tree all to influence graft survival rate.
Wherein, cut-grafting method described in C, comprises the following steps：
Scion bottom is whittled into oblique bevel by C1, and scion retains 1-2 full buds and blocked to 3-5 centimeter lengths；
Stock section is cut out 3-4 centimetres by C2 towards the epidermis of southern side, and depth is advisable just to see xylem, and width is with connecing
Fringe bevel width is consistent；
The bevel of scion is inserted stock by C3, and both forming layers are aligned as far as possible, and interface ties up fixation.
The present invention also provides application of the above-mentioned fruit of a cubeb litsea tree engrafting method on the fruit of a cubeb litsea tree breeds.
Fruit of a cubeb litsea tree engrafting method of the present invention be by the trial of a variety of methods, and field experiment obtain it is optimal
Grafting system.Its inventive point is mainly reflected in the selection and processing of scion, and in the selection of grafting method.
The selection of scion determines the success or failure of fruit of a cubeb litsea tree grafting, may insure using scion acquisition method of the present invention
The vitality of scion, it is greatly enhanced the success rate of grafting.First, the coppice shoot of scion selection root, or sprouted after section bar
Side shoot, has a very strong germination rate, and branch is annual semi-lignified, diameter 3-5 millimeters.Secondly, acquisition time is worked as in the lunar calendar
Year first month of the lunar year November to next year, now fruit of a cubeb litsea tree bud leaf bud do not sprout, the scion of collection will be in certain temperature and wet
Degree is lower to be preserved.Gathered scion grafting time is in the lunar calendar first month of the lunar year to the beginning of February.
Fruit of a cubeb litsea tree complete stool is rich in essential oil, mainly Monoterpenes, has a strong impact on the compatibility between scion and stock, leads
Cause graft survival rate not high.It was found that bud connects, the survival rate of cortex grafting is not high, using cut-grafting method than other grafting methods
Survival rate improves more than 20%, it is clear that cut-grafting method has higher survival rate.
Brief description of the drawings
Fig. 1 is the photo of fruit of a cubeb litsea tree root coppice shoot used in embodiment 1.
Fig. 2 is the photo that the fruit of a cubeb litsea tree used in embodiment 2 cuts after bar the side shoot sprouted.
Fig. 3 be embodiment 3 using the fruit of a cubeb litsea tree tree of the different age of trees as the photo after stock grafting, wherein the right side 1, the right side 2 are stock
The grafting photo survived for 2 years raw fruit of a cubeb litsea tree trees, a left side 1, a left side 2 are that stock is the grafting that 4,5 years raw fruit of a cubeb litsea tree trees do not survive
Photo.
Fig. 4 is that embodiment 4 cuts after bar the side shoot sprouted as scion using the fruit of a cubeb litsea tree, and the grafting survived using cut-grafting method is shone
Piece.
Embodiment
Following examples further illustrate present disclosure, but should not be construed as limiting the invention.Without departing substantially from
In the case of of the invention spirit and essence, the modifications or substitutions made to the inventive method, step or condition belong to the present invention
Scope.
Unless otherwise specified, the conventional meanses that technological means used in embodiment is well known to those skilled in the art.
Embodiment 1 is using root coppice shoot as scion, using cut-grafting method grafting
(1) collection of scion：Using root coppice shoot as scion, photo is shown in Fig. 1, and scion diameter between 3 and 5 millimeters, connects
Fringe semi-lignified.
(2) processing of scion：When leaf bud bud is not sprouted, the time is about in the first month of the lunar year in lunar calendar November to next year.
Locality scion will preserve under certain temperature and humidity, by the scion bottom sealing wax of collection, during (0-10 DEG C) of low temperature preserves,
Buried with wet sand, husky humidity is pinched as group with hand, and hand pine is spread preferably, and the depth of burial is 2/3rds of scion height.Transfer
Scion wax-sealing part is cut off about 3 centimetres before connecing, and scion is immersed in the water diel, allows scion fully to absorb water.
(3) selection of stock：The 50-60 centimetres of trunk that 2 years raw fruit of a cubeb litsea tree tree section bars of Root-stock selection leave is as stock.
(4) selection of time of grafting：Grafting time is in the lunar calendar first month of the lunar year to the beginning of February；
(5) engrafting method：Select cut-grafting method.Scion bottom is whittled into oblique bevel in epidermis smooth place, scion is blocked
To 3-5 centimeter lengths；After stock cuts bar, stock section is cut out 3-4 centimetres towards the epidermis of southern side, depth is just to see xylem
It is advisable, width is consistent with scion bevel width；The scion for retaining 1-2 full buds is rapidly inserted into cut out skin stock it is wooden
Portion, both forming layers are aligned as far as possible, with preservative film colligation interface.
Embodiment 2 is to cut after bar the side shoot sprouted as scion, using bud grafting method grafting
(1) collection of scion：The side shoot sprouted using cutting after bar is as scion, and photo is shown in Fig. 2, and scion diameter is in 3-5 millis
Between rice.
(2) processing of scion：Scion gathers in the beginning of spring, binds up with gauze base segment with wet after harvesting scion, removes blade, when
Its grafting.
(3) selection of stock：The biennial fruit of a cubeb litsea tree tree of Root-stock selection, cut bar and leave 50-60 centimetres of trunk as stock.
(4) selection of time of grafting：Grafting time is in the first month of the lunar year；
(5) engrafting method：Select bud grafting method.A 3-4cm long bevel is being cut in scion at 2cm below tender shoots, in length
The relative opposite side of bevel cuts a 0.5-1cm short bevel.Scion about 1 centimeters above simple bud are cut, and are retained about
10 centimetres of scion.Stock cuts a T otch in main body trunk side, forms the smooth domatic of ligule.By scion
Long bevel along the domatic insertion of stock.Scion can carry out multiple buds on stock trunk and connect, at intervals of 15 cms.By scion
Insert after stock, at preservative film parcel side grafting, wrapped up at eye with individual layer.After side grafting about 2 months, it is seen that eye is sprouted
It is dynamic, pocket knife to cut at eye.Other are sprouted the branch and cut on trunk.
Embodiment 3 is with the stock grafting difference scion of the different age of trees
Scion acquisition method with reference to embodiment 1,2 gathers fruit of a cubeb litsea tree root coppice shoot and cuts after bar the side shoot sprouted, respectively
Bud grafting method and cut-grafting method in 1,2 in conjunction with the embodiments, the stock of the different age of trees is selected to carry out grafting, wherein the mountain with the different age of trees
Grey subtree is stock, and the fruit of a cubeb litsea tree cuts after bar the side shoot sprouted as scion.
Grafting result shows that the selection of stock has a great impact to survival rate, using 2 years raw trunks as stock, mountain
The side shoot sprouted after grey sub- root coppice shoot and section bar is as scion, survival rate greatly 36% (9/25)；Made using 4,5 years raw trunks
For stock, the side shoot sprouted after fruit of a cubeb litsea tree root coppice shoot and section bar survival rate greatly 16% (4/25), is connect as scion using bud
The grafting photo that method is survived and do not survived is shown in Fig. 3.Therefore, using 2 years raw trunks as stock, survival rate is about than using
The stock survival rate of other age of trees is higher by 20%, and this may be more preferably relevant with the compatibility of 2 years raw fruits of a cubeb litsea tree and scion.
4 different scion of embodiment and different grafting methods graft survival rate
Scion acquisition method with reference to embodiment 1,2 gathers fruit of a cubeb litsea tree root coppice shoot and cuts after bar the side shoot sprouted, with master
A biennial common branch on branch and side shoot is control, using the trunk left after biennial fruit of a cubeb litsea tree tree section bar as stock, and
Respectively in connection with the bud grafting method and cut-grafting method in embodiment 1,2,6 groups of experiments are carried out altogether, wherein cutting after bar the side sprouted with the fruit of a cubeb litsea tree
Branch be used as scion, the grafting photo for using cut-grafting method to survive see Fig. 4 (wherein the right side 1, right 2 for stock be 2 years raw fruit of a cubeb litsea tree trees into
Grafting photo living, a left side 1, a left side 2 are that stock is the grafting photo that 4,5 years raw fruit of a cubeb litsea tree trees do not survive.).Every kind of scion is transferred
Connect 35.
Grafting the results are shown in Table 1：The selection of scion has a great impact to survival rate, is sprouted using root coppice shoot or after cutting bar
The side shoot of hair, than being about higher by 20% using common branch survival rate.In addition, it is higher using the survival rate of cut-grafting, connect than bud
About 20% is higher by, this may contain and relevant in the secondary metabolites that bast is rich in fruit of a cubeb litsea tree complete stool, and these are secondary
Metabolin have impact on the compatibility of scion and stock.
1 different scion of table and different grafting methods graft survival rate
| Grafting survival number/grafting sum | Common branch | Root coppice shoot | Cut bar after coppice shoot |
| Bud connects | 4/35 (13%) | 11/35 (31%) | 12/35 (34%) |
| Cut-grafting | 5/35 (14%) | 19/35 (54%) | 18/35 (51%) |
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of fruit of a cubeb litsea tree engrafting method, it is characterised in that the side shoot for sprouting out after selection root coppice shoot or section bar, which is used as, to be connect
Fringe, grafting is carried out with cut-grafting method.
2. fruit of a cubeb litsea tree engrafting method as claimed in claim 1, it is characterised in that comprise the following steps：
A gathers root coppice shoot, or cuts after bar the side shoot for sprouting out as scion, it is desirable to scion semi-lignified, a diameter of 3-5
Millimeter, have some full buds；
The trunk that B leaves after cutting bar using biennial fruit of a cubeb litsea tree tree is stock；
C is in the lunar calendar first month of the lunar year to the beginning of February with cut-grafting method grafting.
3. fruit of a cubeb litsea tree engrafting method as claimed in claim 2, it is characterised in that gathered described in A in the winter time collection or spring adopt
Collection.
4. fruit of a cubeb litsea tree engrafting method as claimed in claim 3, it is characterised in that the winter collection, scion low-temperature moisture preservation are protected
Deposit to next spring grafting.
5. fruit of a cubeb litsea tree engrafting method as claimed in claim 4, it is characterised in that the scion low-temperature moisture preservation preserves, scion bottom
End seal wax, 0-10 DEG C of preservation, is buried with wet sand, and husky humidity is pinched as group with hand, and hand pine is spread preferably, and the depth of burial is scion
2/3rds of height.
6. fruit of a cubeb litsea tree engrafting method as claimed in claim 4, it is characterised in that the next spring grafting, will be connect before grafting
Fringe sealing wax part cuts off 2-4 centimetres, and scion is immersed in the water into diel, allows scion fully to absorb water.
7. fruit of a cubeb litsea tree engrafting method as claimed in claim 3, it is characterised in that the spring collection, scion moisturizing, the same day transfer
Connect.
8. fruit of a cubeb litsea tree engrafting method as claimed in claim 7, it is characterised in that the scion moisturizing is to be bound up with gauze with wet
Base segment, remove blade moisturizing.
9. fruit of a cubeb litsea tree engrafting method as claimed in claim 2, it is characterised in that stock described in B, 50-60 lis is retained after cutting strunk
Meter Gao.
10. application of the fruit of a cubeb litsea tree engrafting method described in claim any one of 1-9 on the fruit of a cubeb litsea tree breeds.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710710914.0A CN107333571A (en) | 2017-08-18 | 2017-08-18 | A kind of fruit of a cubeb litsea tree engrafting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710710914.0A CN107333571A (en) | 2017-08-18 | 2017-08-18 | A kind of fruit of a cubeb litsea tree engrafting method |
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| CN107333571A true CN107333571A (en) | 2017-11-10 |
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| CN201710710914.0A Pending CN107333571A (en) | 2017-08-18 | 2017-08-18 | A kind of fruit of a cubeb litsea tree engrafting method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243755A (en) * | 2018-02-08 | 2018-07-06 | 浙江省林业科学研究院 | A kind of oriental cherry propagation by grafiting method |
| CN108401764A (en) * | 2018-03-02 | 2018-08-17 | 合肥浦邦农业科技有限公司 | A kind of high-yield planting method of the fruit of a cubeb litsea tree |
| CN110447403A (en) * | 2019-08-13 | 2019-11-15 | 中国林业科学研究院亚热带林业研究所 | A kind of Cortex Magnoliae Officinalis propagation by grafiting method |
| CN112075277A (en) * | 2020-08-25 | 2020-12-15 | 衡东县新旺种养农民专业合作社 | Litsea pungens planting method |
| CN114766221A (en) * | 2022-03-28 | 2022-07-22 | 广西壮族自治区中国科学院广西植物研究所 | Economical cultivation method for promoting high yield of litsea cubeba |
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| CN103125243A (en) * | 2013-03-09 | 2013-06-05 | 福建农林大学 | Method for raising seedlings of camellia chrysantha plants by top grafting |
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| CN103125243A (en) * | 2013-03-09 | 2013-06-05 | 福建农林大学 | Method for raising seedlings of camellia chrysantha plants by top grafting |
| CN103125243B (en) * | 2013-03-09 | 2014-07-09 | 福建农林大学 | Method for raising seedlings of camellia chrysantha plants by top grafting |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243755A (en) * | 2018-02-08 | 2018-07-06 | 浙江省林业科学研究院 | A kind of oriental cherry propagation by grafiting method |
| CN108401764A (en) * | 2018-03-02 | 2018-08-17 | 合肥浦邦农业科技有限公司 | A kind of high-yield planting method of the fruit of a cubeb litsea tree |
| CN110447403A (en) * | 2019-08-13 | 2019-11-15 | 中国林业科学研究院亚热带林业研究所 | A kind of Cortex Magnoliae Officinalis propagation by grafiting method |
| CN110447403B (en) * | 2019-08-13 | 2022-04-22 | 中国林业科学研究院亚热带林业研究所 | Magnolia officinalis grafting propagation method |
| CN112075277A (en) * | 2020-08-25 | 2020-12-15 | 衡东县新旺种养农民专业合作社 | Litsea pungens planting method |
| CN114766221A (en) * | 2022-03-28 | 2022-07-22 | 广西壮族自治区中国科学院广西植物研究所 | Economical cultivation method for promoting high yield of litsea cubeba |
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