CN107667689A - A kind of method of quick breeding citrus fruit seedling - Google Patents
A kind of method of quick breeding citrus fruit seedling Download PDFInfo
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- CN107667689A CN107667689A CN201710899013.0A CN201710899013A CN107667689A CN 107667689 A CN107667689 A CN 107667689A CN 201710899013 A CN201710899013 A CN 201710899013A CN 107667689 A CN107667689 A CN 107667689A
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Abstract
The invention discloses a kind of method of quickly breeding citrus fruit seedling, this method is that selection Micropropagated Citrus migrates to cultivation in seedling-raising cup, standby as stock when nursery stock grows newly slightly；From the branch on citrus fruit tree as scion；Using cleft graft by scion and stock grafting；Citrus grafting is cultivated after grafting under suitable illumination and damp condition；After the fringe bud in scion is grown, the tinfoil paper on grafting is divested, and carry out water and fertilizer management and the prevention and control of plant diseases, pest control according to a conventional method.The graft stock that the inventive method is selected is Micropropagated Citrus, well developed root system, with luxuriant foliage and spreading branches in leafy profusion, cane is sturdy, nutrient growth is vigorous, height and rugosity requirement when can reach grafting in shorter seedling raise period needed for stock, greatly improves graft survival rate, and after grafting survival, because stock root system quality is good, grafting recovers fast, and growth is rapid, effectively shorten the seedling time, reduce input cost, remarkable in economical benefits, to accelerate Citrus Industry is fast-developing to provide strong technical support.
Description
Technical field
The invention belongs to fruit tree reproduction technique field, specifically a kind of method of quick breeding citrus fruit seedling.
Background technology
Platymiscium, also known as citrus under citrus (Citrus reticulataBlanco) category Rutaceae.China is citrus
One of important original producton location, citrus aboundresources, improved seeds are various, there is the cultivation history of more than 4000 years.By cultivated since long, choosing
Select, precious fruit of the citrus into the mankind.The body-care material that citrus contains, it is separated to go out more than 30 to plant, wherein mainly having：Class
Flavones, monoterpene, cumarin, carotenoid, class propyl alcohol, acridone, glycerose lipid etc..Citrusfruit edible, orange peel, mandarin orange
Tangerine leaf, Citrus Root can be used as medicine, and have resolving sputum, cough-relieving, relax the difference in functionalitys such as liver, invigorating the spleen, nourishing the stomach, beauty.Citrus nursery stock at present
The method of breeding mainly has：Grafting, cuttage, grow directly from seeds, press strip etc..Wherein mainly based on grafting, propagation by grafiting can tie ahead of time
Fruit, and can keeps the high-quality characteristic of kind, but the citrus nursery stock survival rate that propagation by grafiting at present obtains is relatively low.
The content of the invention
A kind of the problem of present invention exists for existing citrus seedling propagation, there is provided method of quick breeding citrus fruit seedling.
This method is using Micropropagated Citrus as stock, to gather the budling on citrus maternal plant as scion, then stock and scion is carried out
Grafting.This method has simple to operate, and cost is cheap, and survival rate is high, can effectively shorten the cultivation of stock in conventional engrafting method
Time.
In order to realize the above object the technical solution adopted by the present invention is as follows：
A kind of method of quick breeding citrus fruit seedling, including Root-stock selection, scion preparation, seedling grafting, managing after grafting
Process, concrete operation step is as follows：
(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the Micropropagated Citrus of 2-3 months, moves
Plant to the seedling-raising cup equipped with seedling medium, be managed with conventional method for cultivating seedlings, nursery stock grows newly after cultivating 20-30 days
When slightly, you can standby as stock；
(2) scion prepares：From the vigorous, no disease and pests harm of growth, and positioned at plant middle and upper part, the citrus that rugosity is 6-8mm
Branch is wrapped with wet towel after scion is numbered and is put into incubator as scion, then transports interior back and is put into 4 DEG C of refrigerators and treats
With；
(3) seedling grafting：Grafting time was selected in the rainy days in 3-4 months, and grafting uses cleft graft, according to the wood of stock
Matter degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, then used according to citrus scion size
Single-edge blade longitudinally cuts the graft union of a 1-3cm length in stock incision along cross section centre position；From scion base portion 1-3cm
Scion is carried out double-side cutting by place by wedge shape, and cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into
Stock grafting mouth, the principle being aligned with the forming layer at least one side, scion and stock is adjacent to, then using tinfoil paper to graft union
Sealed and fixed, complete grafting；
(4) managing after grafting：Citrus grafting is cultivated after grafting under suitable illumination and damp condition；Grafting 20-30 days
After the fringe bud in scion is grown, the tinfoil paper on grafting is divested, and carry out water and fertilizer management and the prevention and control of plant diseases, pest control according to a conventional method.
Further, step (1) seedling medium is 100 by weight ratio:20-40:10-20:3-6 citrus woods table
Soil, coconut palm chaff, silkworm excrement and slow-release or control-release fertilizer composition.
Further, N-P in the slow-release or control-release fertilizer2O5-K2O ratio is 22-10-10.
Further, step (2) selection born fruit 2-3 citrus branch as scion.
Further, the suitable illumination described in step (4) and humidity are control intensity of illumination 800- 1 week after grafting
1000Lx, Shi Du≤90%；After grafting one week, intensity of illumination 2000-3000Lx, Shi Du≤80% is controlled.
Compared with prior art, advantages of the present invention and have the beneficial effect that：
1st, the graft stock that the present invention selects is Micropropagated Citrus, and well developed root system, with luxuriant foliage and spreading branches in leafy profusion, cane is sturdy, nutrition life
Length is vigorous, height and rugosity requirement when can reach grafting in shorter seedling raise period needed for stock, and degree of lignification is fitted
In, activity is strong, metabolism is vigorous, beneficial to grafting wound healing, graft survival rate is greatly improved, and after grafting survival, due to anvil
Wooden root system quality is good, and grafting recovers fast, and growth is rapid, effectively shortens the seedling time, reduces input cost, economic benefit
Significantly.
2nd, seedling medium provided by the invention can promote Micropropagated Citrus Fast Growth, well developed root system, shorten and cultivate the time,
Good stock is provided for citrus grafting, there is provided the survival rate of grafting.
3rd, the inventive method citrus fruit seedling can not only ensure citrus improved seeds characteristic, and the survival rate of grafting fruit seedling reaches
More than 95%, have a extensive future, to accelerate Citrus Industry is fast-developing to provide strong technical support.
Embodiment
The present invention program is further described below in conjunction with specific embodiment, but is not limited to the guarantor of the present invention program
Protect scope.
Embodiment 1
A kind of method of quick breeding citrus fruit seedling, including Root-stock selection, scion preparation, seedling grafting, managing after grafting
Process, concrete operation step is as follows：
(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the fertile mandarin orange tissue-cultured seedling of 3 months, transplanting
To the seedling-raising cup equipped with seedling medium, it is managed with conventional method for cultivating seedlings, when nursery stock is grown newly slightly after cultivating 25 days,
It is standby to can be used as stock；The seedling medium is 100 by weight ratio：30:20:5 citrus woods table soil, coconut palm chaff, silkworm excrement ease up
Controlled Release Fertilizer forms；N-P in the slow-release or control-release fertilizer2O5-K2O ratio is 22-10-10；
(2) scion prepares：It is from 2 years, the vigorous, no disease and pests harm of growth of having born fruit, and positioned at plant middle and upper part, rugosity
6mm fertile mandarin orange branch is wrapped with wet towel after scion is numbered as scion and is put into incubator, then transported interior back and be put into 4 DEG C
It is stand-by in refrigerator；
(3) seedling grafting：Grafting time was selected in the rainy days in March, and grafting uses cleft graft, according to the wooden of stock
Change degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, it is then single according to citrus scion size
Face blade longitudinally cuts the graft union of a 1-3cm length in stock incision along cross section centre position；At scion base portion 1-3cm
Scion is subjected to double-side cutting by wedge shape, cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into anvil
Wooden graft union, the principle being aligned with the forming layer at least one side, scion and stock are adjacent to, then graft union entered using tinfoil paper
Row sealing and fixed, completion grafting；
(4) managing after grafting：1 week after grafting, intensity of illumination 800Lx, Shi Du≤90% is controlled；After grafting one week, control
Intensity of illumination 2500Lx, Shi Du≤80%；Grafting divests the tinfoil paper on grafting in 24 days after the fringe bud in scion is grown, and
Water and fertilizer management and the prevention and control of plant diseases, pest control are carried out according to a conventional method.
Mandarin orange grafting survival rate is irrigated in the present embodiment to 96.67%.
Embodiment 2
A kind of method of quick breeding citrus fruit seedling, including Root-stock selection, scion preparation, seedling grafting, managing after grafting
Process, concrete operation step is as follows：
(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the Citrus shatangju tissue-cultured seedling of 2 months, moves
Plant to the seedling-raising cup equipped with seedling medium, be managed with conventional method for cultivating seedlings, nursery stock grows newly slightly after cultivating 20 days
When, you can it is standby as stock；The seedling medium is 100 by weight ratio：40:15:4 citrus woods table soil, coconut palm chaff, silkworm excrement and
Slow-release or control-release fertilizer forms；N-P in the slow-release or control-release fertilizer2O5-K2O ratio is 22-10-10；
(2) scion prepares：It is from 3 years, the vigorous, no disease and pests harm of growth of having born fruit, and positioned at plant middle and upper part, rugosity
6mm Citrus shatangju branch is wrapped with wet towel after scion is numbered as scion and is put into incubator, then transported interior back and be put into 4
It is stand-by in DEG C refrigerator；
(3) seedling grafting：Grafting time was selected in the rainy days in March, and grafting uses cleft graft, according to the wooden of stock
Change degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, it is then single according to citrus scion size
Face blade longitudinally cuts the graft union of a 1-2cm length in stock incision along cross section centre position；At scion base portion 1-2cm
Scion is subjected to double-side cutting by wedge shape, cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into anvil
Wooden graft union, the principle being aligned with the forming layer at least one side, scion and stock are adjacent to, then graft union entered using tinfoil paper
Row sealing and fixed, completion grafting；
(4) managing after grafting：1 week after grafting, intensity of illumination 1000Lx, Shi Du≤90% is controlled；After grafting one week, control
Intensity of illumination 2000Lx, Shi Du≤80%；Grafting divests the tinfoil paper on grafting in 20 days after the fringe bud in scion is grown, and
Water and fertilizer management and the prevention and control of plant diseases, pest control are carried out according to a conventional method.
Citrus shatangju grafting survival rate is to 98.47% in the present embodiment.
Embodiment 3
A kind of method of quick breeding citrus fruit seedling, including Root-stock selection, scion preparation, seedling grafting, managing after grafting
Process, concrete operation step is as follows：
(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the navel orange tissue-cultured seedling of 3 months, transplanting
To the seedling-raising cup equipped with seedling medium, it is managed with conventional method for cultivating seedlings, when nursery stock is grown newly slightly after cultivating 30 days,
It is standby to can be used as stock；The seedling medium is 100 by weight ratio：20:15:6 citrus woods table soil, coconut palm chaff, silkworm excrement ease up
Controlled Release Fertilizer forms；N-P in the slow-release or control-release fertilizer2O5-K2O ratio is 22-10-10；
(2) scion prepares：It is from 2 years, the vigorous, no disease and pests harm of growth of having born fruit, and positioned at plant middle and upper part, rugosity
8mm navel orange branch is wrapped with wet towel after scion is numbered as scion and is put into incubator, then transported interior back and be put into 4 DEG C
It is stand-by in refrigerator；
(3) seedling grafting：Grafting time was selected in the rainy days in April, and grafting uses cleft graft, according to the wooden of stock
Change degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, it is then single according to citrus scion size
Face blade longitudinally cuts the graft union of a 2-3cm length in stock incision along cross section centre position；At scion base portion 2-3cm
Scion is subjected to double-side cutting by wedge shape, cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into anvil
Wooden graft union, the principle being aligned with the forming layer at least one side, scion and stock are adjacent to, then graft union entered using tinfoil paper
Row sealing and fixed, completion grafting；
(4) managing after grafting：1 week after grafting, intensity of illumination 800Lx, Shi Du≤90% is controlled；After grafting one week, control
Intensity of illumination 3000Lx, Shi Du≤80%；Grafting divests the tinfoil paper on grafting in 25 days after the fringe bud in scion is grown, and
Water and fertilizer management and the prevention and control of plant diseases, pest control are carried out according to a conventional method.
Navel orange grafting survival rate is to 95.62% in the present embodiment.
Embodiment 4
A kind of method of quick breeding citrus fruit seedling, including Root-stock selection, scion preparation, seedling grafting, managing after grafting
Process, concrete operation step is as follows：
(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the fertile mandarin orange tissue-cultured seedling of 3 months, transplanting
To the seedling-raising cup equipped with seedling medium, it is managed with conventional method for cultivating seedlings, when nursery stock is grown newly slightly after cultivating 30 days,
It is standby to can be used as stock；The seedling medium is 100 by weight ratio：20:10:3 citrus woods table soil, coconut palm chaff, silkworm excrement ease up
Controlled Release Fertilizer forms；N-P in the slow-release or control-release fertilizer2O5-K2O ratio is 22-10-10；
(2) scion prepares：It is from 3 years, the vigorous, no disease and pests harm of growth of having born fruit, and positioned at plant middle and upper part, rugosity
8mm fertile mandarin orange branch is wrapped with wet towel after scion is numbered as scion and is put into incubator, then transported interior back and be put into 4 DEG C
It is stand-by in refrigerator；
(3) seedling grafting：Grafting time was selected in the rainy days in April, and grafting uses cleft graft, according to the wooden of stock
Change degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, it is then single according to citrus scion size
Face blade longitudinally cuts the graft union of a 2-3cm length in stock incision along cross section centre position；At scion base portion 2-3cm
Scion is subjected to double-side cutting by wedge shape, cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into anvil
Wooden graft union, the principle being aligned with the forming layer at least one side, scion and stock are adjacent to, then graft union entered using tinfoil paper
Row sealing and fixed, completion grafting；
(4) managing after grafting：1 week after grafting, intensity of illumination 1000Lx, Shi Du≤90% is controlled；After grafting one week, control
Intensity of illumination 2000Lx, Shi Du≤80%；Grafting divests the tinfoil paper on grafting in 28 days after the fringe bud in scion is grown, and
Water and fertilizer management and the prevention and control of plant diseases, pest control are carried out according to a conventional method.
Mandarin orange grafting survival rate is irrigated in the present embodiment to 97.97%.
Claims (5)
- A kind of 1. method of quick breeding citrus fruit seedling, it is characterised in that：Including Root-stock selection, scion preparation, seedling grafting, transfer The process managed after connecing, concrete operation step are as follows：(1) Root-stock selection：Growth selection is healthy and strong, grows fine, no disease and pests harm, cultivates the Micropropagated Citrus of 2-3 months, migrates to In seedling-raising cup equipped with seedling medium, it is managed with conventional method for cultivating seedlings, nursery stock grows newly slightly after cultivating 20-30 days When, you can it is standby as stock；(2) scion prepares：From the vigorous, no disease and pests harm of growth, and positioned at plant middle and upper part, the citrus branch that rugosity is 6-8mm As scion, wrapped after scion is numbered with wet towel and be put into incubator, then transport back interior be put into it is stand-by in 4 DEG C of refrigerators；(3) seedling grafting：Grafting time was selected in the rainy days in 3-4 months, and grafting uses cleft graft, according to the lignifying of stock Degree, half matter position is selected, stock top is cut at the 1/3 of liftoff height of seedling, then according to citrus scion size one side Blade longitudinally cuts the graft union of a 1-3cm length in stock incision along cross section centre position；Will from scion base portion 1-3cm Scion carries out double-side cutting by wedge shape, and cutting mouth is smooth and cuts to forming layer；The good scion of machining is rapidly inserted into stock Graft union, the principle being aligned with the forming layer at least one side, scion and stock are adjacent to, and then graft union is carried out using tinfoil paper Sealing and fixed, completion grafting；(4) managing after grafting：Citrus grafting is cultivated after grafting under suitable illumination and damp condition；Grafting 20-30 days is waiting After fringe bud on fringe is grown, the tinfoil paper on grafting is divested, and carry out water and fertilizer management and the prevention and control of plant diseases, pest control according to a conventional method.
- 2. the method for citrus fruit seedling is quickly bred according to claim 1, it is characterised in that：Step (1) described seedling medium It is 100 by weight ratio:20-40:10-20:3-6 citrus woods table soil, coconut palm chaff, silkworm excrement and slow-release or control-release fertilizer composition.
- 3. the method for citrus fruit seedling is quickly bred according to claim 2, it is characterised in that：N-P in the slow-release or control-release fertilizer2O5- K2O ratio is 22-10-10.
- 4. the method for citrus fruit seedling is quickly bred according to claim 1, it is characterised in that：Step (2) selection has been born fruit 2-3 citrus branch is as scion.
- 5. the method for citrus fruit seedling is quickly bred according to claim 1, it is characterised in that：It is suitable described in step (4) Illumination and humidity are 1 week after grafting, control intensity of illumination 800-1000Lx, Shi Du≤90%；After grafting one week, control illumination is strong Spend 2000-3000Lx, Shi Du≤80%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243930A (en) * | 2018-03-14 | 2018-07-06 | 宾川川格农业科技有限公司 | A kind of implantation methods of citrus |
| CN108812036A (en) * | 2018-08-20 | 2018-11-16 | 潘国长 | A kind of engrafting and cultivating method of spice all over the mountain |
| CN110100693A (en) * | 2018-03-29 | 2019-08-09 | 湖北格林凯尔精准农业研究院 | A kind of citrus seedling medium and application |
| CN113330929A (en) * | 2021-02-24 | 2021-09-03 | 林为钦 | Citrus seedling growing method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904500A (en) * | 2015-06-01 | 2015-09-16 | 广西壮族自治区林业科学研究院 | Grafting method taking masson pine tissue culture seedlings as stocks |
-
2017
- 2017-09-28 CN CN201710899013.0A patent/CN107667689A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904500A (en) * | 2015-06-01 | 2015-09-16 | 广西壮族自治区林业科学研究院 | Grafting method taking masson pine tissue culture seedlings as stocks |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243930A (en) * | 2018-03-14 | 2018-07-06 | 宾川川格农业科技有限公司 | A kind of implantation methods of citrus |
| CN110100693A (en) * | 2018-03-29 | 2019-08-09 | 湖北格林凯尔精准农业研究院 | A kind of citrus seedling medium and application |
| CN108812036A (en) * | 2018-08-20 | 2018-11-16 | 潘国长 | A kind of engrafting and cultivating method of spice all over the mountain |
| CN108812036B (en) * | 2018-08-20 | 2021-05-11 | 潘国长 | Grafting cultivation method of rhododendron seniavinii |
| CN113330929A (en) * | 2021-02-24 | 2021-09-03 | 林为钦 | Citrus seedling growing method |
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