CN107896670A - The winter rapid propagation method of tribute mandarin orange grafting - Google Patents

The winter rapid propagation method of tribute mandarin orange grafting Download PDF

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CN107896670A
CN107896670A CN201711250087.8A CN201711250087A CN107896670A CN 107896670 A CN107896670 A CN 107896670A CN 201711250087 A CN201711250087 A CN 201711250087A CN 107896670 A CN107896670 A CN 107896670A
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grafting
mandarin orange
seedling
tribute mandarin
scion
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杨瑞巍
陈伏
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Zijin Mountain County De Fu Farm Products Co Ltd
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Zijin Mountain County De Fu Farm Products Co Ltd
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Abstract

The invention discloses a kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, the transplantation of seedlings of tribute mandarin orange tissue culture is cultivated into the seedling-raising cup equipped with seedling medium when tribute mandarin orange grows newly slightly, you can spare as stock;From the autumn growth of fringe tree crown periphery clip semi-lignified is adopted as scion, when grafting, uses cleft graft, and scion and stock are adjacent to, complete grafting;Tribute mandarin orange seedling after grafting is cultivated in seedling bed.The method of the present invention is lifting the grafting of seedling bed progress tribute mandarin orange seedling in winter, can solve citrus and carry out grafting using autumn growth in the winter time, the stock of grafting is used as using tribute mandarin orange tissue-cultured seedling, due to tissue-cultured seedling well developed root system, degree of lignification it is moderate, it is active it is strong, be metabolized it is vigorous, beneficial to grafting wound healing, greatly improve graft survival rate, since stock root system quality is good after grafting survival, grafting recovers fast, growth is rapid, effectively shorten the seedling time of citrus, reduce input cost, remarkable in economical benefits.

Description

The winter rapid propagation method of tribute mandarin orange grafting
Technical field
The present invention relates to seedling propagation technical field, is specifically a kind of winter rapid propagation method of tribute mandarin orange grafting.
Background technology
Citrus (Citrus reticulata Blanco) belongs to platymiscium under Rutaceae, also known as citrus.China is citrus One of important original producton location, citrus aboundresources, improved seeds are various, there is the cultivation history of more than 4000 years.By cultivated since long, Selection, precious fruit of the citrus into the mankind.The body-care material that citrus contains, it is separated to go out more than 30 to plant, wherein mainly having: Flavonoids, monoterpene, cumarin, carotenoid, class propyl alcohol, acridone, glycerose lipid etc..Citrusfruit edible, orange peel, Citrus leaf, citrus root can be used as medicine, and have resolving sputum, cough-relieving, relax the difference in functionality such as liver, invigorating the spleen, nourishing the stomach, beauty.China is the world On Orange Producing big country, Citrus Industry is China's Major agro-industry, and extremely important status is occupied in agricultural economy, Promote to play extremely important effect in increasing peasant income, increase social employment.Tribute mandarin orange is the Natural hybrid of orange and tangerine, has orange Two-fold advantage with tangerine is You Xi farmers' Citrus Cultivars, and tribute mandarin orange authorizes " Chinese name fruit " " Chinese mandarin orange by Chinese fruit circulation association The honorary titles such as king ", there is important economic value.Pure tribute mandarin orange fruit shape is beautiful, fruit coloured gold is yellow, thin skin core is few, the brittle slag of meat, clear Fragrant and sweet honey, high sugar are low sour, with rich flavor, it has concentrated orange class beautiful outline form and citrus fine and tender taste, the two-fold advantage of easy peeling.
The method of citrus seedling propagation mainly has at present:Grafting, cuttage, grow directly from seeds, press strip etc..Wherein citrus is mainly with grafting Based on, grafting can keep the merit of scion variety and the advantageous feature using stock, reach precocity fruiting, enhancing resists The ability of cold, drought resistance, disease and insect resistance etc., moreover it is possible to economic utilization propagating materials, increase nursery stock quantity.On citrus, generally Field graft technology can only carry out grafting using the higher scion branch of aging, degree of lignification, seldom tender, not aging using children Scion branch carry out grafting, otherwise its survival rate is very low.Although the Shoot-tip Grafting or gemmule grafting cultivated in test tube can Grafting is carried out using very tender scion, but it requires stock children tender, and can only be cultivated in test tube.It is tender for citrus The grafting of bud, since citrus tender shoots is the young sprout of citrus plant sprouting soon, young sprout is not yet aging at this time, and degree of lignification is very It is low, it is impossible to realize the grafting of citrus tender shoots well.Therefore, how low cost, high-survival rate, in short time that citrus is excellent It is the major issue for needing to solve now that clone grafting, which is cultivated and planted,.
The disclosure of background above technology contents is only used for inventive concept and the technical solution that auxiliary understands the present invention, it is not The prior art of present patent application is necessarily belonged to, shows the applying date of the above in present patent application in no tangible proof In the case of disclosed, above-mentioned background technology should not be taken to the novelty and creativeness of evaluation the application.
The content of the invention
The present invention for existing citrus seedling propagation there are the problem of, there is provided it is a kind of it is easy to operate, cultivation period is short, grafting The winter rapid propagation method for the tribute mandarin orange grafting that survival rate is high, seedling quality is excellent, seedling cost is low.This method can solve tribute Mandarin orange carries out the technical problem of grafting using autumn growth in the winter time.
In order to realize the above object the technical solution adopted by the present invention is as follows:
A kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, stock is cultivated, Scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth born fruit on tribute mandarin orange maternal plant top is selected as explant, It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after stem section disinfection and grow to 5-8cm to tissue-cultured seedling;
S2:Seedling bed is built:Ground or selection are leeward on the sunny side in cool canopy, the open area of good water permeability builds and be furnished with ground The seedling bed of hot line, the seedling bed can temperature control, control it is wet and control light;
S3:Stock is cultivated:The transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 30-40 days into the seedling-raising cup equipped with seedling medium After when tribute mandarin orange grow it is new slightly when, you can it is spare as stock;
S4:Scion prepares:Select that growth is vigorous, tribute mandarin orange without disease pest, high yield is used as mother plant for cutting, it is peripheral from fringe tree crown is adopted Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of clip 6-10cm, spare;
S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade on stock incision edge Longitudinally cut the graft union of a 1-3cm long in cross section centre position;It is from scion base portion 1-3cm that scion is two-sided by wedge shape progress Cutting, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with least one side The principle of forming layer alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, are completed Grafting;
S6:Cultivated after grafting:By the tribute mandarin orange seedling after grafting controlled in seedling bed under suitable temperature, illumination, humidity into Row is cultivated.
Preferably, seedling bed described in step S2 is lift seedling bed.
Preferably, scion described in step S4 only retains top 1-2 piece leaves, and the blade of reservation is cut into half leaf.
Preferably, the seedling medium described in step S1 is 100 by weight ratio:25-35:10-15:The yellow soil of 4-8, Chinese chestnut Shell, silkworm excrement and slow-release or control-release fertilizer composition.
Preferably, N-P in the slow-release or control-release fertilizer2O5-K2The ratio of O is 22-10-10.
Preferably, step S4 be select to have born fruit 1-2 tribute mandarin orange as mother plant for cutting.
Preferably, the grafting time is 10-11 months.
Preferably, step S3 smears auximone in the binding site of scion and stock.
Preferably, the auximone is indole -3-butyric acid.
Preferably, step S6 controlled at 25-30 DEG C, intensity of illumination 2500-3500Lx, humid control in 70- 80%.
Compared with prior art, advantages of the present invention and have the beneficial effect that:
1st, the present invention can heat the grafting of lifting seedling bed progress citrus in winter, can solve citrus and utilize in the winter time Autumn growth carries out grafting, using tribute mandarin orange tissue-cultured seedling as the stock of grafting, due to tissue-cultured seedling well developed root system, with luxuriant foliage and spreading branches in leafy profusion, cane is sturdy, Nutrient growth is vigorous, height and rugosity requirement when can reach grafting in shorter seedling raise period needed for stock, and lignifying Degree is moderate, it is active it is strong, metabolism is vigorous, beneficial to grafting wound healing, graft survival rate is greatly improved, due to anvil after grafting survival Wooden root system quality is good, and grafting recovers fast, and growth is rapid, effectively shortens the seedling time of citrus, reduces input cost, economy effect It is beneficial notable.
2nd, seedling medium provided by the invention has that preservation capacity of water and fertility is good, and nutrition is comprehensive, and surface is not easy hardened, and weeds are few, The seedling cultivated is sturdy, promotes tribute mandarin orange tissue-cultured seedling Fast Growth, well developed root system, shortens and cultivate the time, provided for tribute mandarin orange grafting Good stock, there is provided the survival rate of grafting.
3rd, this propagation method tribute mandarin orange fruit seedling can not only ensure tribute mandarin orange improved seeds characteristic, and the survival rate of grafting fruit seedling reaches More than 95%, have a extensive future, to accelerate Citrus Industry is fast-developing to provide strong technical support.
4th, the method for the present invention has easy to operate, of low cost, and survival rate is high, can effectively shorten anvil in conventional engrafting method The cultivation time of wood.
Embodiment
The present invention is described in further detail with reference to embodiment.It is emphasized that the description below is only Only it is exemplary, the scope being not intended to be limiting of the invention and its application.
Embodiment 1
A kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, stock is cultivated, Scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth born fruit on tribute mandarin orange maternal plant top is selected as explant, It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after stem section disinfection and grow to 5-6cm to tissue-cultured seedling;
S2:Seedling bed is built:Ground or selection are leeward on the sunny side in cool canopy, the open area of good water permeability builds and be furnished with ground The lift seedling bed of hot line, the seedling bed can temperature control, control it is wet and control light;
S3:Stock is cultivated:After the transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 35 days into the seedling-raising cup equipped with seedling medium When tribute mandarin orange grows newly slightly, you can spare as stock;The seedling medium is 100 by weight ratio:30:12:6 gold zone Soil, chestnut shell, silkworm excrement and slow-release or control-release fertilizer composition.The ratio of N-P2O5-K2O is 22-10-10 in the slow-release or control-release fertilizer.
S4:Scion prepares:Select that growth is vigorous, tribute mandarin orange without disease pest, high yield is used as mother plant for cutting, it is peripheral from fringe tree crown is adopted Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of clip 6-8cm, spare;
S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade on stock incision edge Longitudinally cut the graft union of a 1-3cm long in cross section centre position;It is from scion base portion 1-3cm that scion is two-sided by wedge shape progress Cutting, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with least one side The principle of forming layer alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, are completed Grafting;
S6:Cultivated after grafting:By the tribute mandarin orange seedling after grafting in seedling bed, it is controlled at 28 DEG C, intensity of illumination 3000Lx, humid control are cultivated 75%.
Using 1000 plants of tribute mandarin orange seedlings of method engrafting and cultivating of the present embodiment, arrived through overtesting grafting survival rate 97.6%.
Embodiment 2
A kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, stock is cultivated, Scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth born fruit on tribute mandarin orange maternal plant top is selected as explant, It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after stem section disinfection and grow to 6-8cm to tissue-cultured seedling;
S2:Seedling bed is built:Ground or selection are leeward on the sunny side in cool canopy, the open area of good water permeability builds and be furnished with ground The lift seedling bed of hot line, the seedling bed can temperature control, control it is wet and control light;
S3:Stock is cultivated:After the transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 40 days into the seedling-raising cup equipped with seedling medium When tribute mandarin orange grows newly slightly, you can spare as stock;The seedling medium is 100 by weight ratio:35:15:8 gold zone Soil, chestnut shell, silkworm excrement and slow-release or control-release fertilizer composition.The ratio of N-P2O5-K2O is 22-10-10 in the slow-release or control-release fertilizer.
S4:Scion prepares:Select that growth is vigorous, tribute mandarin orange without disease pest, high yield is used as mother plant for cutting, it is peripheral from fringe tree crown is adopted Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of clip 8-10cm, spare;
S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade on stock incision edge Longitudinally cut the graft union of a 1-3cm long in cross section centre position;It is from scion base portion 1-3cm that scion is two-sided by wedge shape progress Cutting, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with least one side The principle of forming layer alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, are completed Grafting;
S6:Cultivated after grafting:By the tribute mandarin orange seedling after grafting in seedling bed, it is controlled at 30 DEG C, intensity of illumination 3500Lx, humid control are cultivated 80%.
Using 1000 plants of tribute mandarin orange seedlings of method engrafting and cultivating of the present embodiment, arrived through overtesting grafting survival rate 98.2%.
Embodiment 3
A kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, stock is cultivated, Scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth born fruit on tribute mandarin orange maternal plant top is selected as explant, It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after stem section disinfection and grow to 6-8cm to tissue-cultured seedling;
S2:Seedling bed is built:Ground or selection are leeward on the sunny side in cool canopy, the open area of good water permeability builds and be furnished with ground The lift seedling bed of hot line, the seedling bed can temperature control, control it is wet and control light;
S3:Stock is cultivated:After the transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 30 days into the seedling-raising cup equipped with seedling medium When tribute mandarin orange grows newly slightly, you can spare as stock;The seedling medium is 100 by weight ratio:25:12:6 gold zone Soil, chestnut shell, silkworm excrement and slow-release or control-release fertilizer composition.N-P in the slow-release or control-release fertilizer2O5-K2The ratio of O is 22-10-10.
S4:Scion prepares:Select that growth is vigorous, tribute mandarin orange without disease pest, high yield is used as mother plant for cutting, it is peripheral from fringe tree crown is adopted Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of clip 8-10cm, spare;
S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade on stock incision edge Longitudinally cut the graft union of a 1-3cm long in cross section centre position;It is from scion base portion 1-3cm that scion is two-sided by wedge shape progress Cutting, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with least one side The principle of forming layer alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, are completed Grafting;
S6:Cultivated after grafting:By the tribute mandarin orange seedling after grafting in seedling bed, it is controlled at 25 DEG C, intensity of illumination 2500Lx, humid control are cultivated 70%.
Using 1000 plants of tribute mandarin orange seedlings of method engrafting and cultivating of the present embodiment, arrived through overtesting grafting survival rate 96.6%.
Embodiment 4
A kind of rapid propagation method of tribute mandarin orange grafting, including tribute mandarin orange tissue-cultured seedling is cultivated, seedling bed is built, stock is cultivated, Scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth born fruit on tribute mandarin orange maternal plant top is selected as explant, It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after stem section disinfection and grow to 5-7cm to tissue-cultured seedling;
S2:Seedling bed is built:Ground or selection are leeward on the sunny side in cool canopy, the open area of good water permeability builds and be furnished with ground The lift seedling bed of hot line, the seedling bed can temperature control, control it is wet and control light;
S3:Stock is cultivated:After the transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 40 days into the seedling-raising cup equipped with seedling medium When tribute mandarin orange grows newly slightly, you can spare as stock;The seedling medium is 100 by weight ratio:30:15:5 gold zone Soil, chestnut shell, silkworm excrement and slow-release or control-release fertilizer composition.The ratio of N-P2O5-K2O is 22-10-10 in the slow-release or control-release fertilizer.
S4:Scion prepares:Select that growth is vigorous, tribute mandarin orange without disease pest, high yield is used as mother plant for cutting, it is peripheral from fringe tree crown is adopted Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of clip 6-8cm, spare;
S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade on stock incision edge Longitudinally cut the graft union of a 1-3cm long in cross section centre position;It is from scion base portion 1-3cm that scion is two-sided by wedge shape progress Cutting, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with least one side The principle of forming layer alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, are completed Grafting;
S6:Cultivated after grafting:By the tribute mandarin orange seedling after grafting in seedling bed, it is controlled at 30 DEG C, intensity of illumination 3500Lx, humid control are cultivated 75%.
Using 1000 plants of tribute mandarin orange seedlings of method engrafting and cultivating of the present embodiment, arrived through overtesting grafting survival rate 98.1%.
Above content is to combine specific/preferred embodiment further description made for the present invention, it is impossible to Assert that the specific implementation of the present invention is confined to these explanations.Come for general technical staff of the technical field of the invention Say, without departing from the inventive concept of the premise, it can also make some replacements or modification to the embodiment that these have been described, And these are substituted or variant should all be considered as belonging to protection scope of the present invention.

Claims (10)

  1. A kind of 1. rapid propagation method of tribute mandarin orange grafting, it is characterised in that:Including tribute mandarin orange tissue-cultured seedling cultivate, seedling bed build, Stock cultivation, scion preparation, seedling grafting, the process of managing after grafting, concrete operation step are as follows:
    S1:Tribute mandarin orange tissue-cultured seedling is cultivated:The young tender stem-segment with node autumn growth for selecting to bear fruit on tribute mandarin orange maternal plant top is as explant, stem section It can be transplanted when cultivating according to conventional tissue culture seeding cultivating method after disinfection and grow to 5-8cm to tissue-cultured seedling;
    S2:Seedling bed is built:In cool canopy ground or selection it is leeward on the sunny side, the open area of good water permeability build be furnished with ground hot line Seedling bed, the seedling bed can temperature control, control it is wet and control light;
    S3:Stock is cultivated:Treated after the transplantation of seedlings of step S1 tribute mandarin orange tissue cultures is cultivated 30-40 days into the seedling-raising cup equipped with seedling medium When tribute mandarin orange grows newly slightly, you can spare as stock;
    S4:Scion prepares:Select growth it is vigorous, without disease pest, high yield tribute mandarin orange as mother plant for cutting, from adopting fringe tree crown periphery clip Scion is put into bag film and preserves as scion by the autumn growth of the annual semi-lignifieds of 6-10cm, spare;
    S5:Seedling grafting:Cleft graft is used during grafting, according to tribute mandarin orange scion size single-edge blade in stock incision along crosscutting Longitudinally cut the graft union of a 1-3cm long in face centre position;Scion is subjected to two-sided cut by wedge shape from scion base portion 1-3cm Cut, cutting mouth is smooth and cuts to forming layer;The good scion of machining is rapidly inserted into stock grafting mouth, with the shape at least one side The principle of stratification alignment, scion and stock are adjacent to, then graft union is sealed and fixed using plastic film, completes to transfer Connect;
    S6:Cultivated after grafting:Tribute mandarin orange seedling after grafting is controlled in seedling bed and is trained under suitable temperature, illumination, humidity Educate.
  2. 2. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Seedling bed described in step S2 is lifting Formula seedling bed.
  3. 3. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Scion only retains top described in step S4 1-2 piece leaves are held, and the blade of reservation is cut into half leaf.
  4. 4. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Seedling medium described in step S1 by Weight ratio is 100:25-35:10-15:Yellow soil, chestnut shell, silkworm excrement and the slow-release or control-release fertilizer composition of 4-8.
  5. 5. according to the rapid propagation method of claim 4 tribute mandarin orange grafting, it is characterised in that:N-P in the slow-release or control-release fertilizer2O5- K2The ratio of O is 22-10-10.
  6. 6. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Step S4 is to select 1- of having born fruit The tribute mandarin orange of 2 years is as mother plant for cutting.
  7. 7. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:The grafting time is the 11-12 months Part.
  8. 8. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Step S3 is in scion and the knot of stock Close position and smear auximone.
  9. 9. the rapid propagation method of tribute mandarin orange grafting according to claim 1, it is characterised in that:The auximone is Yin Diindyl -3- butyric acid.
  10. 10. according to the rapid propagation method of claim 1 tribute mandarin orange grafting, it is characterised in that:Step S6 is controlled at 25-30 DEG C, intensity of illumination 2500-3500Lx, humid control be in 70-80%.
CN201711250087.8A 2017-12-01 2017-12-01 The winter rapid propagation method of tribute mandarin orange grafting Pending CN107896670A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108811816A (en) * 2018-07-07 2018-11-16 富川凯邦农资经营部 A kind of tribute mandarin orange seedling cuttage breeding method
CN109952873A (en) * 2019-03-27 2019-07-02 广西福桐林业科技有限公司 A kind of method of quick grafting blueness shaddock
CN111631061A (en) * 2020-04-29 2020-09-08 钟山佳园生态农业有限公司 Cultivation method for breeding disease-resistant seedlings of citrus gonggan

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103081699A (en) * 2013-02-06 2013-05-08 广西壮族自治区林业科学研究院 Oil camellia bud seedling stock-grafting propagation method in winter and spring
CN104904500A (en) * 2015-06-01 2015-09-16 广西壮族自治区林业科学研究院 Grafting method taking masson pine tissue culture seedlings as stocks
CN106305268A (en) * 2016-08-18 2017-01-11 重庆市阔园水果种植有限责任公司 Citrus seedling reproduction method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103081699A (en) * 2013-02-06 2013-05-08 广西壮族自治区林业科学研究院 Oil camellia bud seedling stock-grafting propagation method in winter and spring
CN104904500A (en) * 2015-06-01 2015-09-16 广西壮族自治区林业科学研究院 Grafting method taking masson pine tissue culture seedlings as stocks
CN106305268A (en) * 2016-08-18 2017-01-11 重庆市阔园水果种植有限责任公司 Citrus seedling reproduction method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108811816A (en) * 2018-07-07 2018-11-16 富川凯邦农资经营部 A kind of tribute mandarin orange seedling cuttage breeding method
CN109952873A (en) * 2019-03-27 2019-07-02 广西福桐林业科技有限公司 A kind of method of quick grafting blueness shaddock
CN111631061A (en) * 2020-04-29 2020-09-08 钟山佳园生态农业有限公司 Cultivation method for breeding disease-resistant seedlings of citrus gonggan

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Application publication date: 20180413