CN107258629A - It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization - Google Patents
It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization Download PDFInfo
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- CN107258629A CN107258629A CN201710713505.6A CN201710713505A CN107258629A CN 107258629 A CN107258629 A CN 107258629A CN 201710713505 A CN201710713505 A CN 201710713505A CN 107258629 A CN107258629 A CN 107258629A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000004720 fertilization Effects 0.000 title claims abstract description 29
- 238000002347 injection Methods 0.000 claims abstract description 61
- 239000007924 injection Substances 0.000 claims abstract description 61
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000005556 hormone Substances 0.000 claims abstract description 15
- 229940088597 hormone Drugs 0.000 claims abstract description 15
- 230000009027 insemination Effects 0.000 claims abstract description 14
- 238000005516 engineering process Methods 0.000 claims abstract description 9
- 239000012891 Ringer solution Substances 0.000 claims abstract description 6
- 238000005070 sampling Methods 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 16
- 108010000912 Egg Proteins Proteins 0.000 claims description 16
- 210000004681 ovum Anatomy 0.000 claims description 16
- 210000001015 abdomen Anatomy 0.000 claims description 15
- 230000002196 ecbolic effect Effects 0.000 claims description 8
- 210000004907 gland Anatomy 0.000 claims description 8
- 230000001568 sexual effect Effects 0.000 claims description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 210000003746 feather Anatomy 0.000 claims description 5
- 241000252233 Cyprinus carpio Species 0.000 claims description 3
- 241000628997 Flos Species 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 230000026109 gonad development Effects 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 210000001672 ovary Anatomy 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 239000003488 releasing hormone Substances 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 239000002352 surface water Substances 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims 1
- 230000001817 pituitary effect Effects 0.000 claims 1
- 239000004576 sand Substances 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 abstract description 3
- 230000000644 propagated effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010062767 Hypophysitis Diseases 0.000 description 2
- 241000404692 Opistognathidae Species 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 240000000233 Melia azedarach Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The method that many squamas shovel jaw artificial propagation fish rate of fertilization is improved the invention discloses a kind of, is comprised the following steps：(1) parent population prepares；(2) manual injection's hormone：Double injection method of sampling carries out artificial hormone injection, and the time interval of pin injection twice is 24 hours；Raun injection dosage is：First time injection dosage is every kilogram of μ g of parent population injection LRH A 4, and second of injection is every kilogram of μ g+HCG 800IU+PG 35 of parent population injection LRH A 4, and LRH A2 800IU/kg, milter injection dosage is the 1/2 of raun；(3) artificial insemination；(4) micro- running water technology rate of fertilization.The present invention carries out manual injection using double injection method, and interval time is 24 hours.Using semidry method artificial insemination, a certain amount of ringer solution is added, many squamas can be significantly improved and shovel the rate of fertilization of jaw fish artificial propagations, so that solving many squamas shovels the key technology difficulty of jaw fish artificial propagations, and then realizes that seed rearing and marketable fish are propagated artificially and provides safeguard.
Description
Technical field
It is specifically a kind of to improve the side that many squamas shovel jaw artificial propagation fish rates of fertilization the present invention relates to Technique in Fishes fertilization field
Method.
Background technology
According to investigations, the district such as Chongqing City Chengkou County, Wuxi County has a small number of plants to store a number of many squama shovel jaws
Fish (entrance of the city is referred to as city mouthful Lian fishes, Wuxi and is referred to as money fish) parent population, plant can all carry out the artificial of many squama shovel jaw fishes every year
Breeding is attempted, but is not succeeded.Main cause is that embryonated egg is low and open-mouthed bait is not solved well.
Traditional artificial propagation of fish is carried out when water temperature is higher, and corresponding Effect time is shorter.Many squama shovel jaw fishes are more
It is distributed in the flowing water of mountain area, repoductive time is more early, and water temperature is relatively low, therefore otherwise varied, current many squama shovel jaw fish artificial propagations are most
Big shortcoming is that rate of fertilization is low.The low main cause of rate of fertilization is：1st, artificial induced spawning injection of hormone amount and injection time handle
Hold improper；2nd, it is that artificial insemination method is improper.
The content of the invention
It is above-mentioned to solve it is an object of the invention to provide a kind of method for improving many squamas shovel jaw artificial propagation fish rates of fertilization
The problem of many squamas shovel jaw fish artificial propagation rate of fertilization proposed in background technology is low.
To achieve the above object, the present invention provides following technical scheme：
It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, comprise the following steps：
(1) parent population prepares：Water quality is put into preferably to the wild parent population of collection, water velocity is larger, and the tool of water bottom
Have and the temporarily foster cultivation of male and female point pond is carried out in the flowing water pond of grit；Start breeding previous week according to milter seminal concentration,
Meet the scattered degree of moisture and raun belly size, soft degree differentiate its sexal maturity situation, select parent population, and suitably adjust flowing water
Intensity is stimulated, to promote gonad development；The healthy and strong fish without wound, sexual gland fully maturation of selection is used as male and female parent population；
(2) manual injection's hormone：To the parent population chosen, manual injection's oxytocic hormone is carried out, oxytocic hormone is carp brain
Hypophysis PG, luteotropin releasing hormone d-ala analog LRH-A and human chorionic gonadtropin HCG；Sampling double injection method enters pedestrian
Work injection of hormone, the time interval of pin injection twice is 24 hours；Raun injection dosage is：First time injection dosage is every kilogram
Parent population injects the μ g of LRH-A 4, and second of injection is every kilogram of parent population injection LRH-A 4 μ g+HCG 800IU+PG 3-5, LRH-
A2800IU/kg, milter injection dosage is the 1/2 of raun；Male and female parent population is separately put into after injection oxytocic hormone is separated with iron bar
Gutter channel in, micro- stimulation by running water, and every 4 hours check parent population developmental state；
(3) artificial insemination：Wipe parent population body surface water with clean silk floss towel processed, then a people catch raun make its head to
On, belly is aligned down connects ovum basin, and a people gently presses ovary trailing edge with hand along gonopore direction, and fish-egg is flowed into basin, then with same
Method milter sperm is squeezed into basin, by seminal fluid with insemination basin in fish-egg mix, add ringer solution, dosage be fish-egg volume
2 times, being gently mixed with feather is uniformly sufficiently engaged with smart ovum；
(4) micro- running water technology rate of fertilization：Ovum grain is laid in homemade incubator after artificial insemination, and incubator is placed on production
Micro- Lotic hatching is used in ovum groove；Dead ovum, attention control water mold infection, while observing the development feelings of embryo are regularly sorted out daily
Condition.
It is used as further scheme of the invention：The fully ripe visible belly of raun substantially expands sexual gland in step (1),
Sponginess, flexible, gonopore is ruddy；Fully ripe milter rhynchodaenm cone astropyle goes out sexual gland, and the belly raun that compares is small,
Light pressure belly can flow out milky seminal fluid, and can rapidly be scattered in water.
It is used as further scheme of the invention：The parent population chosen in step (2) is injected using pectoral fin base portion.
It is used as further scheme of the invention：Second of injection of raun is every kilogram of parent population injection LRH-A in step (2)
4 μ g+HCG 800IU+PG 4, LRH-A2800IU/kg.
It is used as further scheme of the invention：Due to by parent population maturity, water temperature and injection needle time difference in step (2)
And the influence of injection dosage, many squama shovel jaw fish the second pin time injecting effect times are 46-50h.
It is used as further scheme of the invention：Incubator described in step (4) is wooden body structure, square, 50cm
× 50cm, mesh 0.1mm.
It is used as further scheme of the invention：Start the ovum tool viscosity of fertilization in step (4), one can be sticked into, plumage is used
Hair is gently mixed, it is to avoid be stirred vigorously, and embryonated egg viscosity after water swelling is automatic to disappear.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention carries out manual injection using double injection method,
It it is 24 hours every the time.Using semidry method artificial insemination, a certain amount of ringer solution is added, many squama shovel jaw fish people can be significantly improved
The rate of fertilization of work breeding, so that solving many squamas shovels the key technology difficulty of jaw fish artificial propagation, and then realizes seed rearing and business
Product fish, which is propagated artificially, to provide safeguard.
Brief description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the present invention, this hair
Bright schematic description and description is used to explain the present invention, does not constitute inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 shovels the flow chart of the method for jaw artificial propagation fish rates of fertilization for many squamas of raising.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Referring to Fig. 1, in the embodiment of the present invention, a kind of method for improving many squamas shovel jaw artificial propagation fish rates of fertilization, including
Following steps：
(1) parent population prepares：Water quality is put into preferably to the wild parent population of collection, water velocity is larger, and the tool of water bottom
Have and the temporarily foster cultivation of male and female point pond is carried out in the flowing water pond of grit；Start breeding previous week according to milter seminal concentration,
Meet the scattered degree of moisture and raun belly size, soft degree differentiate its sexal maturity situation, select parent population, and suitably adjust flowing water
Intensity is stimulated, to promote gonad development；The healthy and strong fish without wound, sexual gland fully maturation of selection is used as male and female parent population, sexual gland
The visible belly of the good raun of maturity substantially expands, and sponginess is flexible, and gonopore is ruddy；Maturity of fish gonads is good
Milter rhynchodaenm cone astropyle goes out, and belly compares, and raun is small, and light pressure belly can flow out milky seminal fluid, and can rapidly be dissipated in water
Open；
(2) manual injection's hormone：To the parent population chosen, manual injection's oxytocic drug is carried out, oxytocic hormone is carp brain
Hypophysis (PG), luteotropin releasing hormone d-ala analog (LRH-A) and human chorionic gonadtropin (HCG)；Sampling double injection method
Artificial hormone injection is carried out, the time interval of pin injection twice is 24 hours, and all parent populations are injected using pectoral fin base portion；Raun is noted
Penetrating dosage is：First time injection dosage is every kilogram of parent population injection μ g of LRH-A 4, and second of injection is every kilogram of parent population injection
μ g+HCG 800IU+PG 3-5 of LRH-A 4, LRH-A2800IU/kg, milter injection dosage is the 1/2 of raun；Injection is accelerated the ripening
Male and female parent population is separately put into the gutter channel separated with iron bar after production agent, micro- stimulation by running water, and checks parent population every 4 hours
Developmental state；
(3) artificial insemination：Due to being influenceed by parent population maturity, water temperature and injection needle time different and injection dosage, many squamas
It is 46-50h to shovel the pin time injecting effect time of jaw fish two, wipes parent population body surface water with clean silk floss towel processed, then a people catches
Raun makes its head upwards, and belly is aligned down connects ovum basin, and a people gently presses ovary trailing edge with hand along gonopore direction, and fish-egg is flowed into
In basin, then with same method milter sperm is squeezed into basin, seminal fluid mix with the fish-egg in insemination basin, addition ringer solution,
Dosage is 2 times of fish-egg volume, and being gently mixed with feather is uniformly sufficiently engaged with smart ovum；
(4) micro- running water technology rate of fertilization：After artificial insemination ovum grain be laid in homemade incubator (wooden body structure,
50cm × 50cm squares, mesh 0.1mm), incubator is placed in spawning groove and uses micro- Lotic hatching；Start the ovum tool of fertilization
Viscosity, can stick into one, can be gently mixed with feather, but can not be stirred vigorously, and embryonated egg viscosity after water swelling is automatic
Disappear.Dead ovum, attention control water mold infection, while observing the developmental state of embryo are regularly sorted out daily.
The method of the present invention obtains many squama shovel jaw fish artificial propagation successes in southwest first.The present invention will solve many squamas
Shovel the problem of jaw fish artificial propagation rate of fertilization is low.The present invention's carries out manual injection using double injection method, and interval time is 24
Hour.Using semidry method artificial insemination, add a certain amount of ringer solution, can significantly improve many squamas shovel jaw fish artificial propagations by
Smart rate, so that solving many squamas shovels the key technology difficulty of jaw fish artificial propagation, and then realizes that seed rearing and marketable fish are manually supported
Grow and provide safeguard.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the claim involved by limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (7)
1. a kind of improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that comprises the following steps：
(1) parent population prepares：Water quality is put into the wild parent population of collection preferably, water velocity is larger, and water bottom have sand
Male and female point pond is carried out in the flowing water pond of gravel and temporarily supports cultivation；Starting the previous week of breeding according to milter seminal concentration, chance water
Scattered degree and raun belly size, soft degree differentiates its sexal maturity situation, selects parent population, and suitably adjusts flowing water intensity
Stimulated, to promote gonad development；The healthy and strong fish without wound, sexual gland fully maturation of selection is used as male and female parent population；
(2) manual injection's hormone：To the parent population chosen, manual injection's oxytocic hormone is carried out, oxytocic hormone is carp pituitary
PG, luteotropin releasing hormone d-ala analog LRH-A and human chorionic gonadtropin HCG；Sampling double injection method is manually swashed
Element injection, the time interval of pin injection twice is 24 hours；Raun injection dosage is：First time injection dosage is every kilogram of parent population
The μ g of LRH-A 4 are injected, second of injection is every kilogram of parent population injection LRH-A 4 μ g+HCG 800IU+PG 3-5, LRH-A2
800IU/kg, milter injection dosage is the 1/2 of raun；Male and female parent population is separately put into what is separated with iron bar after injection oxytocic hormone
In gutter channel, micro- stimulation by running water, and every the developmental state of 4 hours inspection parent populations；
(3) artificial insemination：Parent population body surface water is wiped with clean silk floss towel processed, then a people catches raun to make its head upwards,
Belly is aligned down connects ovum basin, and a people gently presses ovary trailing edge with hand along gonopore direction, and fish-egg is flowed into basin, then with same side
Method squeezes into milter sperm in basin, and seminal fluid is mixed with the fish-egg in insemination basin, adds ringer solution, and dosage is the 2 of fish-egg volume
Times, being gently mixed with feather is uniformly sufficiently engaged with smart ovum；
(4) micro- running water technology rate of fertilization：Ovum grain is laid in homemade incubator after artificial insemination, and incubator is placed on spawning groove
It is middle to use micro- Lotic hatching；Dead ovum, attention control water mold infection, while observing the developmental state of embryo are regularly sorted out daily.
2. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(1) the fully ripe visible belly of raun substantially expands sexual gland in, and sponginess is flexible, and gonopore is ruddy；Sexual gland has filled
It is divided into ripe milter rhynchodaenm cone astropyle to go out, belly compares, and raun is small, and light pressure belly can flow out milky seminal fluid, and can be in water
Scatter rapidly.
3. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(2) parent population chosen in is injected using pectoral fin base portion.
4. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(2) second of injection of raun is every kilogram of parent population injection LRH-A 4 μ g+HCG 800IU+PG 4, LRH-A2 800IU/ in
kg。
5. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(2) due to being influenceed by parent population maturity, water temperature and injection needle time different and injection dosage in, many squama shovel jaw fish the second pin time notes
Effect time is penetrated for 46-50h.
6. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(4) incubator described in is wooden body structure, square, 50cm × 50cm, mesh 0.1mm.
7. according to claim 1 improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization, it is characterised in that step
(4) start the ovum tool viscosity of fertilization in, one can be sticked into, be gently mixed with feather, it is to avoid be stirred vigorously, embryonated egg is swollen in water suction
Viscosity is automatic after swollen disappears.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710713505.6A CN107258629A (en) | 2017-08-18 | 2017-08-18 | It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710713505.6A CN107258629A (en) | 2017-08-18 | 2017-08-18 | It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization |
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Family
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| CN201710713505.6A Pending CN107258629A (en) | 2017-08-18 | 2017-08-18 | It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548715A (en) * | 2018-12-05 | 2019-04-02 | 中国海洋大学 | A kind of northern area flower perch whole artificial propagation technology |
| CN113383732A (en) * | 2021-07-27 | 2021-09-14 | 中国水产科学研究院东海水产研究所 | Artificial insemination and incubation method for Oxytropis spinosa juvenile fish |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101960996A (en) * | 2010-10-27 | 2011-02-02 | 北京市水产科学研究所 | Artificial breeding method of Scaphesthes macrolepis fry |
| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN106942096A (en) * | 2016-12-01 | 2017-07-14 | 重庆两江生态渔业发展有限公司 | A kind of many squamas shovel the artificial propagation breeding method of jaw fish |
-
2017
- 2017-08-18 CN CN201710713505.6A patent/CN107258629A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101960996A (en) * | 2010-10-27 | 2011-02-02 | 北京市水产科学研究所 | Artificial breeding method of Scaphesthes macrolepis fry |
| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN106942096A (en) * | 2016-12-01 | 2017-07-14 | 重庆两江生态渔业发展有限公司 | A kind of many squamas shovel the artificial propagation breeding method of jaw fish |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548715A (en) * | 2018-12-05 | 2019-04-02 | 中国海洋大学 | A kind of northern area flower perch whole artificial propagation technology |
| CN113383732A (en) * | 2021-07-27 | 2021-09-14 | 中国水产科学研究院东海水产研究所 | Artificial insemination and incubation method for Oxytropis spinosa juvenile fish |
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Application publication date: 20171020 |
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