CN107258545A

CN107258545A - A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology

Info

Publication number: CN107258545A
Application number: CN201710656014.2A
Authority: CN
Inventors: 王洪学; 许养武; 赵传明; 王洪刚; 张志环; 陈海波; 张新春; 刘征; 陈继祥; 张忠林; 杨玉春
Original assignee: YICHUN ACADEMY OF FORESTRY
Current assignee: YICHUN ACADEMY OF FORESTRY
Priority date: 2017-07-29
Filing date: 2017-07-29
Publication date: 2017-10-20

Abstract

A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology, are related to the matched combined fast breeding nursery stock of blueberry tissue culture bottle seedling fast breeding matrix, belong to technical field of plant asexual propagation.There is provided that a kind of blueberry tissue culture bottle seedling is simple and easy to do, seedling medium --- liver moss consumption is few, cost is low, a kind of blueberry tissue culture bottle seedling simple and fast raising technology that nursery stock rooting rate is high, increment is big.By using simple plastic greenhouse, liver moss, larch pine needle and Nutrition Soil are combined into seedling medium.Tissue-culture container seedling is without hardening, open after bottle, bottle seedling is directly taken out to the section for being cut into 1.5~2cm, with cuttage after " root-growing agent " 500 times of liquid processing 20s, after inserting sunshade net is set up away from ground 50cm, keep matrix liver moss water content in 70% or so (having held water droplet with hand to go out), take root within 15~20 days, rooting rate is up to more than 90%.

Description

A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology

Technical field

The present invention relates to a kind of blueberry tissue culture bottle seedling easy and quick propagating matrix and technology, belong to plant asexual multiplication technology neck Domain.

Background technology

Blueberry (Blueberry)：Also known as cowberry or blue berry, belong to Ericaceae cowberry platymiscium.In Blueberry except Outside conventional sugar, sour and Vc, rich in VE, VA, VB, SOD, ursin, protein, anthocyanin, dietary fibre and abundant K, The mineral elements such as Fe, Zn, Ca.According to surveying and determination, anthocyanin pigments content is up to 163mg, protein 400- in every hectogram blueberry fresh fruit 700mg, fat 500-600mg, carbohydrate 12.3-15.3mg, vitamin A are up to 81-100 international units, VE 2.7- 9.5 μ g, SOD 5.39 international unit, vitamin are all higher than other fruit.Also very high, the calcium 220- in every gram of fresh fruit of trace element 920 μ g, phosphorus 98-274 μ g, magnesium 114-249 μ g, zinc 2.1-4.3 μ g, iron 7.6-30.0 μ g, germanium 0.8-1.2 μ g, copper 2.0-3.2 μ g.Just because of abundant nutritional ingredient is contained in Blueberry, belong to homoamino acid, Gao Xin, high calcium, high ferro, the battalion of homovitamin Support health fruit.It is the third generation small berries for integrating nutrition and health care that international market is generally acknowledged in recent years.And blueberries It is real expensive, widely used, in addition to it can eat raw, it can also be processed into dry fruit, quick-frozen fruit, preserved fruit, fruit wine, jam, fruit juice, ice river in Henan Province Pouring, cake, candy and various drinks etc..

Blueberry is shrub, and tree body size and morphological differences are notable.The height of tree is from 0.3m to 5m, and Vaccinium ashei is up to 10m.For many years Grow thickly, have it is evergreen also have a fallen leaves, single leaf alternate or has sawtooth at leaf full edge.Corolla is in often urniform or bell-shaped.Petal base portion is combined, Outer rim 4 or 5 is split, white or pink, and 8~10, stamen is shorter than style, by insect or anemophily, and inflorescence is generally total shape flower Sequence.Fruit is in black-and-blue when more several ripe, and some kind is red；Fruit has spherical, oval, oblate or pyriform, average fruit Weigh about between 0.5~2.5g, it is maximum up to 5g.Pulp is soft, and many juices, seed is tiny, can be eaten down not with pulp when edible Mouthfeel is influenceed, root system is more very thin, sturdy few, and distribution is shallow, without root hair.

Blueberry can delay memory loss and prevent cardiopathic generation, particularly be regarded with elimination kopiopia, enhancing The effects such as power；Also there is the function such as prevent cranial nerve aging, cardiac stimulant, anticancer softening blood vessel, enhancing human organism immune.

At present, blueberry seedling-wood breeding is generally that tissue-cultured seedling takes outside sprout-cultivating-bottle mode of reproduction.But bred in reproductive process facility, Mating system is not quite similar, and liver moss, more than laying depth 12cm are used cutting medium more.And liver moss is the distinctive vegetation of wetland, Vital effect is played in its protection to wetland, once destroyed is difficult to recover.In recent years, due to blueberry seedling-wood breeding Fast-developing, price increase, is driven by interests, and liver moss exhaustion to the greatest extent, wetland destruction is serious.Because liver moss is that blueberry nursery stock is numerous Optimum substrate during educating, currently without alternative material.Therefore, in order to reduce the usage amount of liver moss, protecting ecology ring Border, it is necessary to made an effort in being applied in combination for matrix, reduces the usage amount of liver moss.

At present, relevant " blueberry epicormic branch cuttage and outside sprout-cultivating-bottle technology " has been reported, but content related to the present invention is not Appear in the newspapers and lead, also without associated operational procedure and technical standard.Conventional technical requirements facility is complicated, and cost is high, skill Art is cumbersome, it is necessary to which hardening, seedling medium consumption is big, and nursery stock rooting rate is low, and increment is small.

The content of the invention

The purpose of the present invention is to provide a kind of blueberry tissue culture bottle seedling fast numerous matrix and technology for above-mentioned situation, blueberry group Train outside sprout-cultivating-bottle radication substrate combination collocation technology.

Expand numerous nursery stock, epicormic branch cuttage mainly in tissue culture outside sprout-cultivating-bottle (epicormic branch cuttage is applicable this technology).By setting up Simple plastic greenhouse, is applied in combination using liver moss, larch pine needle, Nutrition Soil, so as to reach reduction liver moss usage amount, improves skewer Insert the purpose of rooting rate and increment.

Brief description of the drawings

(blueberry tissue culture bottle seedling breeds schematic diagram) is described in further detail to patent of the present invention below in conjunction with the accompanying drawings.

Brief description：After bed surface is carried out, cuttage is carried out, sunshade net is then set up, watered in good time, in good time weeding.

Fig. 1 is that blueberry tissue culture bottle seedling breeds Nutrition Soil；

Fig. 2 is that blueberry tissue culture bottle seedling breeds cuttage and seedling culture；

Fig. 3 is that blueberry tissue culture bottle seedling is bred cuttage and finished；

Fig. 4 is that blueberry tissue culture bottle seedling is bred and sets up sunshade net；

Fig. 5 is that blueberry tissue culture bottle seedling breeding nursery stock survives situation；

Fig. 6 is that blueberry tissue culture bottle seedling breeds seedling growth situation then.

Embodiment

1st, easy vinyl house is set up, and is carried out in it.

2nd, build sunning water pool or replaced with other containers such as vat.

3rd, using flat furrow cuttage and seedling culture mode.

4th, it will be flattened first in canopy, by the wide 40cm of pavement, retain the wide 1~1.2m of bed surface and carry out laying pavement.

5th, liver moss will dry, smash, and length is generally no greater than 5cm；Pine needle will be picked except debris such as branch, broad-leaveds, before laying 2a is sterilized with 800 times of carbendazim, then pulls out and drains laying.

6th, the preparation of Nutrition Soil：Every cubic metre of fertile soil+5kg composite fertilizer or fertile soil are with the pig manure become thoroughly decomposed by 3: 1: mixed Close.

7th, furrow face is turned over, depth 20cm, 5cm Nutrition Soils is laid above, Nutrition Soil is away from pavement 5cm.

8th, the uniform thick larch pine needles of paving 2cm on Nutrition Soil；Re-lay the dry liver mosses of 3cm.

9th, tissue culture blueberry bottle seedling is transported into place, opens bottle, seedling is taken out with tweezers, culture medium cutting is close to scissors. Then bottle seedling is cut into the section of 2~3cm length again, is pulled out and is put into vessel with 500 times " root-growing agent " immersion 20s, then use sharp mouth Tweezers are clipped in the liver moss completed by 3 × 3cm distance insertion, and 2~3cm of depth, the above is cut to flush with liver moss and is defined.

10th, watered in time after being plugged, and the sunshade net of light transmittance 75% is set up away from earth's surface 50cm in bed surface.

11st, during every morning 7, watered in right amount each 1 time during afternoon 3, air humidity more than 90%, water content of substrate 60~ 70% (held and dripped with hand), temperature control removes the weeds of bed surface at any time at 25~40 DEG C.

12nd, take root within 10~20 days, remove sunshade net within 30 days or so.Short clump blueberry more than Seedling height 12cm, Second Year then More than 27cm；Half high clump blueberry high more than 13cm, more than Second Year 48cm then.Rooting rate more than 90%.

Claims

1. blueberry tissue culture bottle seedling fast numerous matrix and technology, it is characterized in that facility is simple, seedling medium is greatly saved, cost is low, Rooting rate is high, increment is big.

2. according to blueberry tissue culture bottle seedling fast numerous matrix and technology described in claim 1, it is characterized in that facility is simple, simple plastics Greenhouse just can be with.

3. according to blueberry tissue culture bottle seedling fast numerous matrix and technology described in claim 1, it is characterized in that Nutrition Soil, battalion are laid in furrow bottom Soil laying 2cm larch pine needles above are supported, 3cm a thin layer liver mosses are re-layed above, liver moss 3/4 is saved.

4. according to blueberry tissue culture bottle seedling fast numerous matrix and technology described in claim 1 or 2, it is characterised in that can be in greenhouse, letter Carried out in easy vinyl house, play a part of heating moisturizing, set up sunshade net regulation illumination after cuttage on bed surface.

5. blueberry tissue culture bottle seedling fast numerous matrix and technology, its is topmost to be characterised by after taking root that root system is deep into Nutrition Soil at once In " have milk ", sufficient nutrient is provided for root system development and ground Stem nematode in time.Be greatly promoted seedling survive and Grow.

6. blueberry tissue culture bottle seedling fast numerous matrix and technology, it is characterised in that tissue-culture container seedling opens bottle cuttage, skewer without hardening Rooting rate is inserted more than 90%, then short more than the clump blueberry 12cm of Seedling height, more than Second Year 27cm；Half high clump blueberry 13cm with On, more than Second Year 48cm.It can be used for field planting.