CN102524061A - Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area - Google Patents
Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area Download PDFInfo
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- CN102524061A CN102524061A CN2011103965012A CN201110396501A CN102524061A CN 102524061 A CN102524061 A CN 102524061A CN 2011103965012 A CN2011103965012 A CN 2011103965012A CN 201110396501 A CN201110396501 A CN 201110396501A CN 102524061 A CN102524061 A CN 102524061A
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- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 22
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 22
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 22
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 22
- 238000005516 engineering process Methods 0.000 title claims abstract description 13
- 238000009395 breeding Methods 0.000 title claims abstract description 10
- 230000001488 breeding effect Effects 0.000 title claims abstract description 10
- 238000005520 cutting process Methods 0.000 title abstract 11
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 4
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 4
- 241000018646 Pinus brutia Species 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 238000009423 ventilation Methods 0.000 claims abstract description 4
- 239000002023 wood Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 210000004185 liver Anatomy 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000001902 propagating effect Effects 0.000 abstract 2
- 241000195940 Bryophyta Species 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000007670 refining Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000003716 rejuvenation Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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Abstract
The invention discloses a rapid breeding technology for blueberry tissue culture seedling micro cuttings at a high and cold area. The rapid breeding technology comprises the following steps of: cutting blueberry tissue culture seedlings clustered by secondary culture into micro cuttings of 1.5-2 cm, placing in rooting solution, soaking for 10-30 seconds and fishing out; cutting into a matrix seed bed with layered pine needles and mosses in multi-span intelligent greenhouses, sunlight greenhouses and ground cold sheds; controlling temperature to be 13-30 DEG C, keeping humidity at 90-60 percent and performing shading 3000-10000LX and ventilation to promote rooting growth; then scissoring young stems of rooted, survived and grown approximately semi-ligneous seedlings from 1-1.5 cm under near surface; scissoring micro cuttings by taking the stem between two knots as a section to perform in-vitro young stem micro cutting; and then bowl-feeding, transplanting and growing the seedlings survived and grown through micro cutting to obtain community seedlings. The rapid breeding technology for the blueberry tissue culture seedling micro cuttings at the high and cold area has the characteristics of simple blueberry tissue culture seedling production process steps, short production period, capability of changing the bottle seedling rooting environment conditions from complex into simple and capability of propagating a blueberry tissue culture seedling micro cutting by 25-30 times in one year in a mode of propagating seedlings with seedlings, and is the rapid breeding technology which is suitable for the blueberry tissue culture seedling micro cuttings of the high and cold area.
Description
Technical field: the present invention relates to a kind of cultivating seedlings technology, specifically the little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood.
Background technology: present, have traditional blueberry tissue cultivating seedling production status now, the one, the interior rejuvenation rooting process time of blueberry tissue cultivating seedling bowling pin is long, and generally rootage duration is more than 2 months in bottle, and takes root in the bottle and need under aseptic condition, strictness operate; The 2nd, this mode not only expends man-hour, consumes the material and the energy that a subculture is made, and the back refining shoot survival percent of taking root in the bottle is merely 30%～70%.
Summary of the invention: the objective of the invention is to; Shortening rejuvenation rooting process in the blueberry tissue cultivating seedling bowling pin, is a little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood that program refining shoot survival percent is high with taking root, tame the refining seedling in the tissue cultivating seedling rejuvenation bottle and transplanting three integration procedures.Its technology contents is that the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm, places taking root liquid; Soak 10-30 and pull out second, cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth, adopts temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation; The hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is one section miniature slotting fringe of clip near surface 1-1.5cm clip children stem with 2 internodes; The young stem that exsomatizes is little to be inserted; Then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth, can become the commodity nursery stock.The present invention compares its advanced part and is with prior art; The little slotting fast breeding technology of this high and cold area blueberry tissue cultivating seedling wood; Have blueberry tissue cultivating seedling production routine step and simplify, the production cycle foreshortened to about 60 days by 120 days, had reduced to take root in the bottle and need to expend in man-hour of sterile working and reduced material and energy resource consumption that a subculture is made; Bottle seedling rooting environmental condition is become simply from complicacy; In addition, little insert " with the Miao Fanmiao " of strain blueberry tissue cultivating seedling wood can breed 25-30 characteristics doubly in 1 year, thus overcome in the tissue cultivating seedling bottle in existing traditional blueberry tissue cultivating seedling production routine take root, indoor refining seedling, the greenhouse is transplanted and production routine such as nursery transplanting in; Existing bottle seedling rooting environmental condition is complicated; Taking root in the bottle needs strict operation under aseptic condition, consumes the material and the energy, time-consuming that a subculture is made, the low weak point of blueberry group training refining shoot survival percent.
Embodiment: the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm, places taking root liquid, soaks 10-30 and pulls out second; Cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth; Adopt temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation, the hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is near surface 1-1.5cm clip children stem; With 2 internodes is one section miniature slotting fringe of clip; The young stem that exsomatizes is little to be inserted, and then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth; Can become the commodity nursery stock, more than just constitute the present invention.
Claims (1)
1. the little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood is characterized in that the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm; Place taking root liquid, soak 10-30 and pull out second, cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth; Adopt temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation; The hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is one section miniature slotting fringe of clip near surface 1-1.5cm clip children stem with 2 internodes; The young stem that exsomatizes is little to be inserted; Then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth, can become the commodity nursery stock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110396501 CN102524061B (en) | 2011-12-03 | 2011-12-03 | Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110396501 CN102524061B (en) | 2011-12-03 | 2011-12-03 | Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area |
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| CN102524061A true CN102524061A (en) | 2012-07-04 |
| CN102524061B CN102524061B (en) | 2013-09-04 |
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| CN 201110396501 Expired - Fee Related CN102524061B (en) | 2011-12-03 | 2011-12-03 | Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636373A (en) * | 2013-11-04 | 2014-03-19 | 大连大学 | Method for vegetative propagation of vaccinium plants through fresh sphagnum |
| CN103733834A (en) * | 2013-12-16 | 2014-04-23 | 湖北省牧童蓝莓科技有限公司 | Cultivation method for interplanting fritillaria between lines of blueberry plants |
| CN103875529A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Blueberry tissue culture propagation and ex-vitro rooting method |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN104025832A (en) * | 2013-09-17 | 2014-09-10 | 天津市林业果树研究所 | Green branch cuttage method for blueberries in Tianjin area |
| CN104221690A (en) * | 2014-09-29 | 2014-12-24 | 上海植物园 | Fast breeding method for bouquet camellia single-bud cutting |
| CN104429849A (en) * | 2014-11-13 | 2015-03-25 | 大连大学 | New method for water planting of blueberries |
| CN104542210A (en) * | 2014-11-28 | 2015-04-29 | 大连大学 | Rapid propagation method for cranberry plant |
| CN105075777A (en) * | 2015-08-24 | 2015-11-25 | 山东省烟台市农业科学研究院 | Blueberry moss seedling breeding method |
| CN107258545A (en) * | 2017-07-29 | 2017-10-20 | 伊春林业科学院 | A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
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| JP2003304765A (en) * | 2002-04-17 | 2003-10-28 | Gumma Prefecture | Blueberry seedling raising method |
| JP2008220242A (en) * | 2007-03-12 | 2008-09-25 | Univ Of Miyazaki | Raising seedlings |
| CN101690452A (en) * | 2009-09-23 | 2010-04-07 | 金陵科技学院 | Hardwood cutting and seedling raising method for blueberry |
| CN102138529A (en) * | 2011-03-31 | 2011-08-03 | 中国农业大学 | Quick rooting method for 'Heyun' blueberry seedlings formed by tissue culture |
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2011
- 2011-12-03 CN CN 201110396501 patent/CN102524061B/en not_active Expired - Fee Related
Patent Citations (4)
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| JP2003304765A (en) * | 2002-04-17 | 2003-10-28 | Gumma Prefecture | Blueberry seedling raising method |
| JP2008220242A (en) * | 2007-03-12 | 2008-09-25 | Univ Of Miyazaki | Raising seedlings |
| CN101690452A (en) * | 2009-09-23 | 2010-04-07 | 金陵科技学院 | Hardwood cutting and seedling raising method for blueberry |
| CN102138529A (en) * | 2011-03-31 | 2011-08-03 | 中国农业大学 | Quick rooting method for 'Heyun' blueberry seedlings formed by tissue culture |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025832A (en) * | 2013-09-17 | 2014-09-10 | 天津市林业果树研究所 | Green branch cuttage method for blueberries in Tianjin area |
| CN103636373A (en) * | 2013-11-04 | 2014-03-19 | 大连大学 | Method for vegetative propagation of vaccinium plants through fresh sphagnum |
| CN103733834A (en) * | 2013-12-16 | 2014-04-23 | 湖北省牧童蓝莓科技有限公司 | Cultivation method for interplanting fritillaria between lines of blueberry plants |
| CN103733834B (en) * | 2013-12-16 | 2015-11-25 | 湖北省牧童蓝莓科技有限公司 | The cultivation method of a kind of blueberry row interplant bulb of fritillary |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN103875529A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Blueberry tissue culture propagation and ex-vitro rooting method |
| CN103875515B (en) * | 2014-02-18 | 2017-01-04 | 杭州佑国生物科技有限公司 | A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method |
| CN104221690A (en) * | 2014-09-29 | 2014-12-24 | 上海植物园 | Fast breeding method for bouquet camellia single-bud cutting |
| CN104221690B (en) * | 2014-09-29 | 2016-11-23 | 上海植物园 | A kind of quick-breeding method of Shu Huacha flower single-bud cuttage |
| CN104429849A (en) * | 2014-11-13 | 2015-03-25 | 大连大学 | New method for water planting of blueberries |
| CN104542210A (en) * | 2014-11-28 | 2015-04-29 | 大连大学 | Rapid propagation method for cranberry plant |
| CN104542210B (en) * | 2014-11-28 | 2017-01-25 | 大连大学 | A rapid propagation method for cranberry plants |
| CN105075777A (en) * | 2015-08-24 | 2015-11-25 | 山东省烟台市农业科学研究院 | Blueberry moss seedling breeding method |
| CN107258545A (en) * | 2017-07-29 | 2017-10-20 | 伊春林业科学院 | A kind of blueberry tissue culture bottle seedling fast numerous matrix and technology |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
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| CN102524061B (en) | 2013-09-04 |
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