CN102524061A

CN102524061A - Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area

Info

Publication number: CN102524061A
Application number: CN2011103965012A
Authority: CN
Inventors: 孟庆彬; 韦庆和
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-12-03
Filing date: 2011-12-03
Publication date: 2012-07-04
Anticipated expiration: 2031-12-03
Also published as: CN102524061B

Abstract

The invention discloses a rapid breeding technology for blueberry tissue culture seedling micro cuttings at a high and cold area. The rapid breeding technology comprises the following steps of: cutting blueberry tissue culture seedlings clustered by secondary culture into micro cuttings of 1.5-2 cm, placing in rooting solution, soaking for 10-30 seconds and fishing out; cutting into a matrix seed bed with layered pine needles and mosses in multi-span intelligent greenhouses, sunlight greenhouses and ground cold sheds; controlling temperature to be 13-30 DEG C, keeping humidity at 90-60 percent and performing shading 3000-10000LX and ventilation to promote rooting growth; then scissoring young stems of rooted, survived and grown approximately semi-ligneous seedlings from 1-1.5 cm under near surface; scissoring micro cuttings by taking the stem between two knots as a section to perform in-vitro young stem micro cutting; and then bowl-feeding, transplanting and growing the seedlings survived and grown through micro cutting to obtain community seedlings. The rapid breeding technology for the blueberry tissue culture seedling micro cuttings at the high and cold area has the characteristics of simple blueberry tissue culture seedling production process steps, short production period, capability of changing the bottle seedling rooting environment conditions from complex into simple and capability of propagating a blueberry tissue culture seedling micro cutting by 25-30 times in one year in a mode of propagating seedlings with seedlings, and is the rapid breeding technology which is suitable for the blueberry tissue culture seedling micro cuttings of the high and cold area.

Description

The little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood

Technical field: the present invention relates to a kind of cultivating seedlings technology, specifically the little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood.

Background technology: present, have traditional blueberry tissue cultivating seedling production status now, the one, the interior rejuvenation rooting process time of blueberry tissue cultivating seedling bowling pin is long, and generally rootage duration is more than 2 months in bottle, and takes root in the bottle and need under aseptic condition, strictness operate; The 2nd, this mode not only expends man-hour, consumes the material and the energy that a subculture is made, and the back refining shoot survival percent of taking root in the bottle is merely 30%～70%.

Summary of the invention: the objective of the invention is to; Shortening rejuvenation rooting process in the blueberry tissue cultivating seedling bowling pin, is a little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood that program refining shoot survival percent is high with taking root, tame the refining seedling in the tissue cultivating seedling rejuvenation bottle and transplanting three integration procedures.Its technology contents is that the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm, places taking root liquid; Soak 10-30 and pull out second, cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth, adopts temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation; The hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is one section miniature slotting fringe of clip near surface 1-1.5cm clip children stem with 2 internodes; The young stem that exsomatizes is little to be inserted; Then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth, can become the commodity nursery stock.The present invention compares its advanced part and is with prior art; The little slotting fast breeding technology of this high and cold area blueberry tissue cultivating seedling wood; Have blueberry tissue cultivating seedling production routine step and simplify, the production cycle foreshortened to about 60 days by 120 days, had reduced to take root in the bottle and need to expend in man-hour of sterile working and reduced material and energy resource consumption that a subculture is made; Bottle seedling rooting environmental condition is become simply from complicacy; In addition, little insert " with the Miao Fanmiao " of strain blueberry tissue cultivating seedling wood can breed 25-30 characteristics doubly in 1 year, thus overcome in the tissue cultivating seedling bottle in existing traditional blueberry tissue cultivating seedling production routine take root, indoor refining seedling, the greenhouse is transplanted and production routine such as nursery transplanting in; Existing bottle seedling rooting environmental condition is complicated; Taking root in the bottle needs strict operation under aseptic condition, consumes the material and the energy, time-consuming that a subculture is made, the low weak point of blueberry group training refining shoot survival percent.

Embodiment: the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm, places taking root liquid, soaks 10-30 and pulls out second; Cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth; Adopt temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation, the hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is near surface 1-1.5cm clip children stem; With 2 internodes is one section miniature slotting fringe of clip; The young stem that exsomatizes is little to be inserted, and then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth; Can become the commodity nursery stock, more than just constitute the present invention.

Claims

1. the little slotting fast breeding technology of high and cold area blueberry tissue cultivating seedling wood is characterized in that the blueberry tissue cultivating seedling of growing thickly successive transfer culture is cut into the long miniature slotting fringe of 1.5-2cm; Place taking root liquid, soak 10-30 and pull out second, cuttage adds liver moss and is layered as on the matrix seedbed to pine needle in connecting an intelligent greenhouse, greenhouse by solar heat and the cold canopy of the earth; Adopt temperature control 13-30 degree, the 90%-60% that preserves moisture, shade 3000-10000LX and ventilation; The hestening rooting growth, the nearly semi-lignified seedling that will taking root becomes to live again grows is one section miniature slotting fringe of clip near surface 1-1.5cm clip children stem with 2 internodes; The young stem that exsomatizes is little to be inserted; Then, little nursery stock that is inserted into the growth of living is irritated alms bowl transplant growth, can become the commodity nursery stock.