KR100453216B1 - The method cultivation and germination seed sapling of korana nakal - Google Patents

Abstract

The present invention relates to a method for germinating barn seed seeds for germinating seeds of a bark tree (earth) growing in mountains and fields to grow into real seedlings. More specifically, the step of picking and sorting the fruit hanging on the barn tree in autumn, the step of mixing sand and fine soil in the seedling box, and the selected barn tree fruit in the pulp state or the pulp Seeding the barn tree in the seedling box containing the sand and soil, and covering the ground, seeding the seedling box in which the barn fruit is sown in a well-ventilated shade for 17 months, and springing the dormant seedling box. Spraying enzymes consisting of microorganisms and water on a weekly basis from the time of germination, and growing to a size enough to germinate seedlings sown in the seedling box so that they can be transplanted into a field and grown in large quantities. The present invention can produce a large amount of seedlings of the bark tree and at the same time supply a pure native bark tree to live a healthy life It is invented to enjoy.

Description

Method of cultivating seed germination seedlings of the bark tree {The method cultivation and germination seed sapling of korana nakal}

The present invention relates to a method for germinating seed of a barn tree seed for germinating seeds of a barn tree (earth) growing in a mountain and field to grow into a real seedling. More specifically, the fruit on the barn tree is harvested in the fall, mixed with sand in the state of pulp or covered with pulp, dormant until spring after one year, and then sowing to grow seedlings. The present invention relates to a method for cultivating seed germination seed of barn seedlings which is invented so as to supply strong seedlings.

The barn tree of the present invention is called the earth in one shot, and is a broad leaf-sized tree that leaves as a spruce tree (buckthorn) and a plant. It grows up to 10-20m long, grows up to 1m in diameter, and leaves are wide egg-shaped, resembling a mulberry leaf. White flowers bloom in June and ripen in October-November. The fruit looks unusual and resembles coral or chicken claws. The taste was sweet, so I called wood wheat (木 蜜) in wood, and in China, it was called Hyunpori (으로), which means pear from fresh garden, and it was called Baekseokmok (白石 木) because it is hard as a stone. The fruit ripens brown, with three seeds in each of the three rooms. Seeds are brown and the shell is hard, shiny and slightly flat.

The barn tree composed of the above is composed of fruit, roots, branches, leaves, glucose, malic acid, calcium, and other components that are beneficial to the human body such as furanguranine, hobenin, hobenside, and habenic acid. It is known as a tree that has a therapeutic effect on recovery and vomiting.

Researchers have discovered that "amperopsin and hobenitins", extracted from ferns, protects the liver and eliminates alcoholism and hangovers.

Looking at the contents described in the literature that has been told about the efficacy of such a barn tree from ancient times,

In Hyeopjung Herbal Medicine , the decoction of the decoction of the leaf is severely boiled and has the same effect as the fruit, and it is effective in relieving vomiting and releasing 酒 毒.

In herbal medicine, it is effective in treating symptoms that are hard to come out, dry mouth and faeces,

In the herbaceous oil , it quenches thirst, removes swelling , moisturizes the intestines , helps excrete urine, removes heat from the upper diaphragm, and has effects like oysters.

In Jinnam honcho, removing the poison and soaking in alcohol to relax muscle strength and fluids (액) well, and when taking children to control parasites, and 脾.

In the primordial possession , a man drunk thirty years, had a severe fever, and was close to the color, extremely debilitating. So I tried to release the poison by putting the roots in the medicine to protect the blood donation, but there was no difference at all, but when I finally put the bark tree about a month, the fever went down, my energy was restored, and the poison was released. And

In the medicinal herb, some people who lived in the south used wood to repair their homes, and accidentally dripped the wood chips into alcohol, and after a few days the liquor became watery.

In Uibang-dong ( a book written in King Sejong) , a person named Geyoung-shin who lived in Nissan had several horses drinking water every day with small stalks, buckled in rice, and had medicines to fix small stalks. When the doctor made a vow, the musk man was soaked in a liquor and put it in a jar of earth-fruit decoction.

Asked the cause, “Sogal is a disease caused by the spleen's decay and kidney failure, which prevents the spleen from suppressing the water and raising the fluid. However, this man's veins are very hot in the spleen, but the kidneys are not so weak.

This person drinks too much alcohol and is spoiled by the spleen. This bottle is not sogal but a wine bottle.

Musk detoxifies all kinds of fruits. Sesame trees also get rid of alcohol. Therefore, "The two drugs were used together to get rid of alcohol."

In this way, the barn tree is treated with extracts from fruits, roots, branches, and leaves to detoxify the toxicity of organs.

Therefore, in modern times, industrial society develops and various diseases are caused, and the individual's life becomes rich, so he has a considerable interest in health care and eats any food that is beneficial to the body. While the bark tree, which is known to heal the disease and activate the liver function, is in danger of being depleted, while the increasing demands occur, efforts are currently being made to cultivate the bark tree (earth) in large quantities. In addition, there are many researches on the method of extracting the medicinal effect, and the general public is increasing interest in the bark tree.

Therefore, if you look at the method of cultivating the barn tree so far, there is a general method of cultivating the barn by sprinkling the seed and grafting the seed, but this method is a seeding failure and dormant treatment of seeds so that germination fails or smooth folding Not only does it happen, but its growth is slowing so many difficulties in mass production.

Conventional patent No. 331178 "mass propagation method of seedling tree seedling" developed in view of such a point, "the step of selecting the faithful seed by the repair method for the good fruit of the seedling tree population, Remove the moisture and leave at room temperature for 2 to 3 days to dry the seeds, put the seeds in a container to soften the seed by adding 35% sulfuric acid, and wash the seeds with running water by separating the softened seeds from sulfuric acid And swelling the pear and the endosperm in the seed with water by immersing in running water for 12 to 36 hours after removing the seed seedlings, and mixing the swollen seed with wet sand in a 1: 1 ratio at 4 ° C. Cold humidification for 8 weeks, select germicidal seeds for disinfection and sterilize them in a petri dish covered with wetlands and germinate them in an incubator; Consists of auxin to promote proliferation of mass flow method heotgae tree seedling, it characterized in that it comprises the steps of which the cuttings from the cuttings and soil keeping the temperature and humidity of the propagation in bulk cuttings immersed in hormone solutions ".

The mass breeding method of these seedlings is to promote the germination of seeds by treatment with sulfuric acid, and to collect fruit from about 10 well-deficient individuals in a native hut family, collect seeds from the end of the fruit, and repair the water by the repair method. For seed germination, seed is used to settle seeds. To remove moisture, remove seeds with paper towel and leave at room temperature for 2 ~ 3 days to dry seeds. Add seeds below / 10 and pour 35% industrial sulfuric acid (H ₂ SO ₄ ) in a volume that will allow the seeds to soak. To mix the seeds and sulfuric acid well, immerse for 1 to 3 hours while stirring with a glass rod to soften thick seedlings. At the end of this process, wash the seeds with running water to remove the sulfuric acid and sulfuric acid from the seedlings, and then place the seeds in running water for 12 to 36 hours (24 hours on average). Allow to swell.

The swelled seeds were mixed with wet sand in a 1: 1 ratio and cold-treated at 4 ° C. for 4 weeks, 6 weeks, and 8 weeks, and then selected seeds after germination promoting treatment to remove seeds invaded by fungus. After 10 minutes of disinfection in 20% Lax (Corox-Clarax Co., Ltd.) solution, petri dishes with water-saturated wetlands were used for 3 treatments and 50 seeds per repetition for each treatment. Seeds are germinated by incubating in an incubator (Non-warrior, Korea) that maintains 25 ± 2 ℃ temperature, 2,000 lux, and 16 hours of light condition in a dish. If moisture remains, sulfuric acid reacts with moisture to produce an exothermic reaction and burns, thus not only germinating seeds, but also increasing the rate at which seeds die due to uneven thickness of the seed during corrosion of the seed. Many, also sulfur After sedimentation, the seeds are washed with water to remove the sulfuric acid and the corroded part of the seedlings and the swelling is caused by the trace amount remaining. Therefore, the germinated shoots die when the seeds germinate. Although it can be carried out for research purposes in the laboratory, seedling germination methods for cultivation in mass production are practically difficult problems.

The present invention has been invented in view of the above problems, by mixing the bark fruit with sand to dormant for 17 months to increase the germination rate to grow the bark tree in large quantities to supply sufficient to the consumer at the same time of the native (local) The purpose is to preserve the essence of the barn tree and to prevent extinction.

The present invention having such an object is the step of picking and picking the fruit hanging on the barn tree in autumn, the step of mixing sand and fine soil in the seedling box, and the selected barn tree fruit in the pulp state or pulp Seeding the barn tree fruit in a seedling box containing sand and soil in a covered state, and covering the seedling box in which the barn tree seed is sown for 17 months in a well-ventilated shade, and dormant treatment When the seedling box is grown in spring and germination, spraying enzymes consisting of microorganisms and water on a weekly basis, and when the seedlings seeded in the seedling box are grown to a size enough to germinate and transplant, It is to be able to supply large quantities.

1 is a cultivation process of germinating seedlings fruit seedlings according to the present invention.

Figure 2 is a process of germinating the bark fruit according to the present invention in a sack.

Hereinafter, an embodiment of the present invention will be described in detail.

Harvesting and sorting the fruits of the 4 to 5 year old native barn trees grown in the wild;

Mixing 50% of sand and 50% of fine soil and placing them in a seedling box 2 cm to 5 cm thick;

And sprinkling the fruit of the barn tree at intervals of 0.2 to 0.5 cm in a seedling box containing sand and soil in the state where the selected barn tree fruit is in the pulp state or the pulp;

Mixing 50% of sand and 50% of soil on the fruit sprinkled at intervals of 0.2 to 0.5 cm, covering 1 to 2 cm,

Dormant for 17 months in a well-ventilated shade of the seedling box seeded with the barn fruit,

Spraying enzymes composed of Bacillus microorganisms (sold by the Korea Organic Farmers Association) and water, which are commercially available, on a weekly basis from the time of germination of the dormant seedlings that have been dormant for 17 months;

If the seedlings seeded on the seedling box are grown to a size enough to be germinated and transplanted, the seedlings are transplanted into a field and grown in a seedling tree.

The enzyme is sprayed by diluting for 30 minutes by mixing 100g of commercially available Bacillus microorganisms and 15g of sulfur sugar in 500L of water.

Selection of a suitable planting place of the fern cultivated by the seedling germination method as described above,

First, choose an oily creek or a well-drained paddy field.

Second, choose areas that are not dry and not too windy.

Third, choose organic pastures, fields or cleared land rich in organic matter.

Fourth, choose a place that is semi-shaded and wet due to abundant humus.

Fifth, it is most suitable for places that are adjacent to Yasan, which cannot be mechanized and difficult to access agricultural machinery.

When planting after selecting a suitable plantation of the barn tree as described above, when planting the barn tree seedlings, when the moisture of the ground is moist, plant it immediately; otherwise, if the soil is dry, water the soil and soak the roots in water.

An example of a method for cultivating a seed germination seed of a native seed barn fern of the present invention made as described above will be described in detail.

(Example 1)

Example when the fruit of the barn tree is collected and dormant in the presence of pulp

Step 1: Harvest and Select Fruits

In the fall of mid-October to early-November, we collect barn hung on wild 4-5 year old native barn trees.

At this time, the seed of the barn is screened with pure pulp, which removes twigs, leaves, and contaminants with the flesh.

Second step: mixing and sowing

The selected barn fruit is mixed in a proportion of 50% of fine sand and 50% of soil that is not contaminated with a pulp.

The mixed sand and soil are placed in a seedling box 2 cm to 5 cm thick.

The amount of sand and soil contained in the seedling box is somewhat different depending on the size of the seedling box, but is filled with a thickness of 2 cm to 5 cm based on the size of the seedling box in the market.

The seedlings of the selected barn tree in the state of pulp and sprinkling the seedlings of the barn tree at intervals of 0.2 to 0.5cm in the seedling box containing the sand and soil, and then sowing the sand mixed with 50% sand and 50% soil on the sprinkled fruit 1 Cover with -2cm.

Seeds of huts sown as described above are used as nutrients until germination because the flesh is mixed with sand during the dormant period.

Step 3: dormancy and germination

The seedling box seeded with the bark fruit is left dormant for 17 months in an airy shade.

The dormant seedlings that have been dormant for 17 months are sprayed with enzymes by diluting for 30 minutes by mixing 100 g of Bacillus microorganisms and 15 g of brown sugar in commercially available 500 L water every week from the time of spring and germination.

The microorganism was used in the market or Korea Organic Agricultural Cooperatives as a nutrient for promoting germination, Bacillus (Bacilus) was used.

Step 4: Transplant and Grow

If the seedlings seeded in the seedling box are grown to a size enough to be germinated and transplanted, the seedling trees are grown by transplanting them into fields.

Seedlings of the barn tree germinated from the seedling box can be transplanted in the spring when usually about 10 cm.

In addition, in the present invention, the size of the seedling at the time of transplantation is 10 cm or more is suitable and does not necessarily limit the size.

In addition, the bark tree is up to 10-20m tall and can grow more than 1m in diameter, but considering that fruit can be harvested from 4-5 years of age, the trees and trees can The distance can be planted at intervals of 4-5m. If seedlings are to be sold later, they can be transplanted at intervals of 10cm to 20cm.

(Example 2)

Example when the fruit of the barn tree is collected and dormant in a seeded state,

Step 1: Fruit Extraction and Screening

In the autumn of mid-October to early-November, the fruit is gathered from four to five-year-old native barn trees grown in the wild.

At this time, the seed of the barn tree selects the pure barn tree fruit from which the twigs, leaves, and contaminants are removed while the pulp is present.

Second step: mixing and sowing

The selected hut fruit is mixed with the seeds in the pulp state at a rate of 50% uncontaminated fine sand and 50% fine soil.

The mixed sand and soil are placed in a seedling box 2 cm to 5 cm thick.

The amount of sand and soil contained in the seedling box is somewhat different depending on the size of the seedling box, but is filled with a thickness of 2 cm to 5 cm based on the size of the seedling box in the market.

The seedlings of the selected barn tree in the state covered with the flesh and seedlings in the seedling box containing the sand and soil at intervals of 0.2 ~ 0.5 ㎝ sowing and seeding the sand mixed with 50% sand and 50% soil on the sprinkled fruit 1 Cover with -2cm.

Step 3: dormancy and germination

The seedling box seeded with the bark fruit is left dormant for 17 months in a well-ventilated shade.

The dormant seedlings that have been dormant for 17 months are sprayed with enzyme diluted by diluting for 30 minutes by mixing 100 g of Bacillus microorganisms and 15 g of brown sugar in commercially available 500 L water every week from the time of spring and germination.

Step 4: Transplant and Grow

If the seedlings seeded in the seedling box are grown to a size enough to be germinated and transplanted, the seedling trees are grown by transplanting them into fields.

The difference between the above-mentioned Example 1 and Example 2 differs only in the state which put on the flesh and not the flesh, and the remainder was implemented on the same conditions.

(Example 3)

In Examples 1 and 2, a seedling box is used to dormant the fruit of the barn, whereas in Example 3, an embodiment in which sand and berries are mixed and dormant is treated in a bag.

First step: picking and sorting fruit

As in the above example, the fruit hanging on the wild hut or cultivated huts of 4-5 years old, grown in the wild, is harvested in the fall of mid-October to early November.

At this time, the seed of the barn tree selects the pure barn tree fruit from which the twigs, leaves, and contaminants are removed while the pulp is present.

Second step: mixing

The selected hut fruit is mixed so that the ratio of fine sand and hut tree seeds that are not contaminated with a pulp is 3: 1.

The mixture is mixed in a container of a predetermined capacity so that the ratio of the uncontaminated fine sand and the fruit of the nut tree is 3: 1 and mixed evenly so that the fruit is not exposed to the sand surface.

In the above embodiment, the fruit of the barn fruit is never peeled off and mixed with fine sand with pure fruit or pulp covered with pulp.

Step 3: Sleep

After mixing the fine sand and the fruit of the barn tree, soak 20 kg in pure hemp sack and leave for 1 year in airy shade to sleep.

In the dormant treatment step, the storage bag containing the fine sand and the fruit of the barn tree is the most suitable for the hemp formed of pure hemp, but other bags having the same properties as the hemp can be used. It is advisable to use the fruit of the barn from the month 25 to the beginning of November.

Step 4: Sowing

Seeds harvested in the fall and treated dormant for one year are in the process of dormant in a culture vessel in a natural neglect or natural neglected environment, the fruit pulp is mixed by itself, leaving only sand and hut seed.

The seeding of the barn tree seeds is sown in the field between the beginning of November and the end of November, when the bark seeds are mixed with sand.

The field where the hut seed is sown is also formed with a ridge in a pure field that is not polluted, and the seed shed with a mixture of sand and hut seed.

The sowing is sprinkled with two to three times the seed on the top surface of the seed on the ridges of the field mixed with sand and mixed with hut.

Step 5: grow

The seedlings planted between the beginning of November and the end of November enter the preparation stage of the germination in the field until the next spring, and the germination starts when the spring begins and the frozen ground is thawed.

At this time, the seedless bark tree seeds not subjected to dormancy treatment as described above are not germinated even when spring comes and very few germinations are made, but this is not hardly any.

Seeds of the shed trees sown as described above are used as nutrients until germination because the flesh is mixed with sand during the dormant period.

The seeded huts are sprayed with enzyme every week from the time of spring and germination.

The enzyme is sprayed by diluting for 30 minutes by mixing 100 g of microorganisms and 15 g of brown sugar in 500 L of water.

Crop microorganisms added to the enzyme is used commercially available.

Seedlings grown and grown as described above is enough to be transplanted again to grow other fields to grow.

(Example 4)

Example 4 is also carried out in the same steps as in the process of Example 3 Example 3 is in the state of the pulp, in Example 4 mixed with the sand in the pulp seeds seeded state is carried out as follows Done.

First step: picking and sorting fruit

As in the above example, the fruit hanging on the wild hut or cultivated huts of 4-5 years old, grown in the wild, is harvested in the fall of mid-October to early November.

At this time, the seed of the barn tree selects the pure barn tree fruit from which the twigs, leaves, and contaminants are removed while the pulp is present.

Second step: mixing

The seed of the selected bark fruit is mixed so that the ratio of the fine sand and the organza seed unpolluted in the seeded state is 3: 1.

The mixture is mixed in a container of a certain capacity so that the ratio of the uncontaminated fine sand and the shed seeds is 3: 1 and evenly mixed so as not to expose the fruit to the sand surface.

Step 3: Sleep

After mixing the fine sand and the hut seed, soak 20kg in a bag made of pure hemp and leave to sleep for 1 year in a well-ventilated shade.

The dormant treatment period is one year, but it is preferable to use the fruit of the barn tree collected from October 25 to early November of autumn.

Step 4: Sowing

Sowing of dormant seeds harvested for one year after the fall is sown in the field in the state of the barn seeds mixed with sand between the beginning of November and the end of November.

The sowing is sprinkled with a seed of the barn tree mixed with sand on the ridge of the field and covered with two to three times the seeds on the top surface.

Step 5: grow

The seedlings sown between the beginning of November and the end of November enter the preparation stage of the germination in the field until the next spring, and the germination begins when the spring begins and the frozen ground is thawed.

The sown seedlings are sprayed with enzyme every week from spring and germination.

The enzyme is sprayed by diluting for 30 minutes by mixing 100 g of microorganisms and 15 g of brown sugar in 500 L of water.

Seedlings grown and grown as described above is grown and transplanted to another field when enough to transplant.

Embodiments of the present invention carried out in the above are all made of the same step, but Example 1 and Example 2 are used in the same dormant container as a seedling box, in Example 1 in the state of the pulp of the fruit of the hut when dormant The seedlings are sown, and in Example 2, only the seeds are sown in the seedlings with the flesh of the bark fruit.

In addition, in Example 3 and Example 4, the dormant container is used in the same manner as the bag. Also, in Example 3, the dormant treatment is carried out with the flesh of the fruit of the bark, and the embodiment 4 is the state of the fruit of the barn. As a seed dormant only.

As described above, the seedlings germinated in the seedling box or the field are transplanted and the seedlings grown by 10 cm or more can be sold to the public.

The choice of suitable plantation for the finished barn seedlings is as follows: oily streams or well-drained paddy fields, poorly dry and windy areas, organic pastures or fields or clearings, rich in humus The most suitable place is a semi-shaded, wet place, which is impossible to be mechanized and difficult to access to agricultural machinery, which is adjacent to Yasan.

The method of planting after selecting a suitable plantation of the hut tree as described above, planting waste-rich pasture land with a cultivator, tractor, etc. can be planted, but where it is difficult to farm field crops because there is no organic matter, manure compost, organic compost, complex fertilizer Fertilize.

When planting barn seedlings, plant the soil immediately when it is moist. Otherwise, if the soil is dry, water the soil and soak the roots in water.

If drought continues after planting in dry soil, watering should be done to increase seedling sticking rate.

(Comparative Example 1)

In Examples 1 and 2, Example 1 is seeded in a seedling box in which sand and soil are mixed one-to-one with the fruit pulp during dormant processing, and the upper surface is 1-2 cm. After 17 months of dormancy in a well-ventilated shade, it is in a state of maintaining the tenderness and moisture while mixing the flesh, so that the germination rate is higher than 90% and the root is strong.

As in Example 2, the seeds of the bark fruit were sown in a seedling box in which sand and soil were mixed in a one-to-one manner, and the top surface was covered with 1 to 2 cm, followed by 17 months in a well-ventilated shade. In the case of dormant treatment, the germination rate was slightly different depending on the seedling box, but it was maintained at 90%. Could know.

(Comparative Example 2)

In Example 1, the seedlings were sown in a seedling box with the pulp of the fruit of the barn, and then dormant for 17 months in the shade. If the seedlings are kept in a shade with good air circulation for one year and sown in the field in the fall, and then germinated in the spring, they are all mixed with sand and dormant, so the probability of germination is negligible. However, both examples showed a good germination rate of more than 90%, both roots were good.

(Comparative Example 3)

In Example 3 and Example 4, the same result as in Comparative Example 1 was obtained.

That is, in Example 3, when the pulp of the bark fruit is in the state of mixing with sand at a ratio of 3: 1, putting it on a sack and dormant sowing in the fall, and peeling the bark of the bark fruit as in Example 4 After seeding and mixing only seeds with sand in a ratio of 3: 1, dormant treatment and sowing in autumn, the rate of germination in the following spring is similar, but the method of Example 3 is slightly higher in germination and rooting The strength was also strong.

As can be seen from the comparative example above, the dormant treatment for cultivation of the barn tree can be cultivated in large quantities even if any one of the embodiments 1 to 4 is used, but the fruit pulp is present. When mixed with sand and stored in the process of not only prevents the water evaporation of seeds, but also provides a mixture of nutrients to nourish the germination in the spring 17 months later was found to be more smooth.

In this way, the present invention allows the seedling production of a large number of seedlings within a short time by creating a new seeding method and a cultivation method while preserving the essence of the native tree (local).

The present invention as described above is a method of cultivating the seed germination seedlings of the conventional barn tree seedlings are mixed with fine sand and germinated in the seedling box to grow germination in the spring of the next year and transplanted and grown After soaking for 1 year, it is sown in the fall of 1 year. When seeds are sown in the existing field, seeds do not form, and even when cutting, the roots are not well formed, which causes difficulty in breeding. In addition to being able to produce large quantities of seedlings and supplying only pure native bark trees, it has the effect of preventing the deterioration of competitiveness due to laboratory facility costs, labor costs, and long-term investment, and enhancing the competitiveness of commodities.

Claims (4)

Harvesting and sorting the fruits of the 4 to 5 year old native barn trees grown in the wild; Mixing 50% of sand and 50% of fine soil and placing them in a seedling box 2 cm to 5 cm thick; Selecting one of the selected state of the barn fruit with the flesh or the state covered with the pulp sowing the fruit of the barn tree at intervals of 0.2 to 0.5 cm in the seedling box containing sand and soil, Mixing 50% of sand and 50% of soil on the fruit sprinkled at intervals of 0.2 to 0.5 cm, covering 1 to 2 cm, Dormant for 17 months in a well-ventilated shade of the seedling box seeded with the barn fruit, Spraying enzymes consisting of Bacillus microorganisms and water, which are commercially available, on a weekly basis from the time of germination of the dormant seedlings that have been dormant for 17 months; The method of cultivating the seedling seedlings seedlings seedlings seedlings seedlings seedlings are grown to a size enough to germinate and transplanted to the field. delete The method of claim 1, The dormant treatment of the seedling box in which the hut fruit is sown is mixed so that the fine sand and the fruit of the hut tree are 3: 1 so that they can be sown in the field in autumn after being dormant for 1 year under the same conditions as the outdoor environment. Method of cultivating seedling seedlings. The method according to any one of claims 1 to 3, Enzyme to be sprayed when the seed germination, 500 g of water, Bacillus (Bacilus) microorganisms commercially available 100g, 15g of brown sugar mixed and diluted for 30 minutes characterized in that the seedling seedling seed germination cultivation method.