KR102560631B1 - Method for increasing germination ratio of Hovenia dulcis seed - Google Patents

Abstract

The present invention comprises the steps of (a) tearing the seed coat of barnacle seeds; (b) alternating temperature treatment of the corrugated seed coat; (c) pre-cooling the seed coat treated at different temperatures; And (d) it relates to a method for increasing the germination rate of seeds of Hovenia raisin, comprising the step of treating the pre-cold storage seed coat with a growth regulator. By establishing a technique that can effectively increase the germination rate through the method for increasing the germination rate of Hovenia raisins, it can contribute to the increase in income of the Hovenia raisins producing farms.

Description

Method for increasing the germination rate of Hovenia dulcis seeds {Method for increasing germination ratio of Hovenia dulcis seed}

The present invention relates to a method for enhancing the germination rate of seeds of Hovenia raisin, and more particularly, to a method for enhancing the germination rate of seeds of Hovenia raisin, which is subjected to a water absorption treatment for removing the seed coat, and to a temperature treatment and a growth regulator treatment for the moisture-absorbed seeds.

Hovenia dulcis var. koreana is a deciduous broad-leaved tree of the dicotyledonous buckthorn tree buckthorn family, growing up to 8-10m in height and 40-80cm in diameter. The flowers of the barn tree bloom around June, and the fruits are harvested around October-November, and are round and brown.

Hovenia tree has been proven to lower blood alcohol concentration and is known to relieve thirst caused by hangovers. According to <Bonchogangmok>, a medical book written by Lee Shi-jin in the Ming Dynasty of China, it is said that if you are addicted to alcohol due to excessive drinking, you can treat it by decocting the stem of the barnacle tree. Even in modern times, barn tree is in the limelight as a health food, and accordingly, the market is expanding, centering on the beverage industry, such as hangover drinks, coffee drinks, and soy milk using barn bar tree.

The physiological and ecological characteristics of the barn tree are not known, and there are many difficulties in cultivation, such as low germination rate of seeds, so that the demand cannot be met. Seeds such as Hovenia japonica have thick and hard seed coats, so it is judged that dormancy continues due to water absorption or oxygen absorption and physical obstacles in the seed coat.

In addition, in the case of seeds with thick and hard seed coats, germination often begins when the seed coat is removed, but even after removal of the seed coat, a problem has been identified that germination does not occur if the temperature is not appropriate (Non-Patent Document 1).

Republic of Korea Patent Publication No. 10-2022-0053244

Korean J. Medicinal Crop Sci. 9(2): 166 -172 (2001)

An object of the present invention is to provide a method capable of effectively increasing the germination rate of seeds of barnacles.

In addition, it is intended to use the above method not only to increase the income of barn tree producing farms, but also to establish conservation measures for forest genetic resources.

The present invention, in order to achieve the above object, (a) the step of breaking the seed coat of the seeds of barberry tree; (b) alternating temperature treatment of the corrugated seed coat; (c) pre-cooling the seed coat treated at different temperatures; and (d) treating the pre-cooled seed coat with a growth regulator.

In one embodiment of the present invention, the seed coat wave may be performed by immersing the barnacle seeds in sulfuric acid (H ₂ SO ₄ ).

In one embodiment of the present invention, the immersion time may be 60 to 180 minutes.

In one embodiment of the present invention, the alternating temperature treatment may be to lower the temperature from 20 ~ 25 ℃ to 10 ~ 15 ℃.

In one embodiment of the present invention, the alternating temperature treatment may be to lower the temperature from 25 ℃ to 15 ℃.

In one embodiment of the present invention, the alternating temperature treatment may be to lower the temperature from 20 ℃ to 10 ℃.

In one embodiment of the present invention, the pre-cooling storage may be performed at 1 to 5 ° C for 20 to 40 days.

In one embodiment of the present invention, the growth regulator may be Gibberellin; GA ₃ .

In one embodiment of the present invention, the treatment concentration of the growth regulator may be 0.1 ~ 1000 ppm.

There has been a problem in that technology for improving the germination rate has not yet been established when artificially cultivating the barn tree, which is a valuable tree species for medicinal and beverage purposes in Korea. Accordingly, by establishing a technique that can efficiently increase the germination rate through the method for increasing the germination rate of the barnacle barberry seed according to the present invention, it can contribute to increasing the income of barn barberry producing farms.

In addition, the propagation and preservation of valuable genetic resources that are gradually disappearing due to development and overfishing will be more efficiently performed, and the produced seedlings are expected to be planted in the wild habitat to restore the damaged wild habitat, and at the same time, it can be used to establish conservation measures for forest genetic resources due to excessive deforestation.

Figure 1 shows the water absorption rate according to the treatment time of barn tree sulfuric acid (H ₂ SO ₄ ).
Figures 2a and 2b show the germination rate according to alternating temperature treatment, Figure 2a shows the germination rate of the control group (general seeds), Figure 2b shows the germination rate of the treated group (sulfuric acid (H ₂ SO ₄ ) treatment 60 minutes).
Figures 3a and 3b show the germination rate according to the growth regulator treatment, Figure 3a is the germination rate of the control (general seeds), Figure 3b is the germination rate of the treatment (sulfuric acid (H ₂ SO ₄ ) treatment 60 minutes).

The present invention comprises the steps of (a) tearing the seed coat of barnacle seeds; (b) alternating-temperature treatment of the seeds whose seed coats are broken; (c) pre-cooling the seed treated at a different temperature; And (d) it relates to a method for enhancing the germination rate of hovenia raisins seeds, comprising the step of treating the pre-cooled seeds with a growth regulator.

The (a) seed coat breaking step is to increase the moisture absorption rate into the seed coat by immersing the seeds of Hovenia japonica in strong acid, preferably sulfuric acid (H ₂ SO ₄ ) to break the seed coat.

The concentration of the sulfuric acid (H ₂ SO ₄ ) may be 90 to 99% (v/v), preferably 95 to 99% (v/v), and more preferably 98% (v/v), but is not limited thereto. If the concentration is less than 90% (v/v), the seed coat is not efficiently wavy, and there is a limit to increasing the water absorption rate into the seed coat.

The immersion time may be 60 minutes or more, preferably 60 to 180 minutes. When the immersion time is less than 60 minutes, the seed coat is not efficiently wavy, and there is a limit to increasing the water absorption rate into the seed coat. On the other hand, when the immersion time exceeds 180 minutes, there is a problem of inefficiency because the breaking efficiency of the seed coat no longer increases.

In the step of (b) alternating temperature treatment, seed germination is greatly affected by the germination ability of the seed itself and the environment of the sowing spot, and in the case of the germination ability of the seed itself, it is closely related to spontaneous dormancy and temperature. Since there are seasonal characteristics and diurnal temperature differences in Korea, this is to adjust the seasonal factors and temperature conditions similarly.

The alternating temperature treatment may be to lower the temperature from 20 to 25 ° C to 10 to 15 ° C, preferably from 25 ° C to 15 ° C, or from 20 ° C to 10 ° C, more preferably from 25 ° C to 15 ° C. It can be lowered, but is not limited thereto. In the case of the alternating temperature treatment from 25 ° C to 15 ° C, germination appears from the second week, and a germination rate of about 100% may be exhibited at 5 weeks.

The (c) pre-cooling storage step is a method for breaking seeds with germination rate and dormancy according to seed storage, and germination can be promoted by breaking dormancy by adjusting the balance of germination inhibitory substances and germination promoting substances of seeds. 20 to 40 days at 1 to 5°C, preferably 25 to 35 days at 4 to 5°C, and more preferably 30 days at 5°C.

If it is out of the above range, there is a problem that the predetermined purpose of pre-cooling storage cannot be achieved.

The (d) growth regulator treatment step is to break dormancy by increasing the germination promoter rather than the germination inhibitor by supplying the germination promoter directly to the seed, and the growth regulator may be Gibberellin (GA ₃ ), cytokinin, benzyl adenine or kinetin, but is not limited thereto.

The treatment concentration of the growth regulator may be 0.1 to 1000 ppm, preferably 10 to 1000 ppm, but is not limited thereto.

Hereinafter, examples and the like will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention can be modified in many different forms, and the scope of the present invention should not be construed as being limited to the following examples. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

<Example>

1. Materials and Methods

(One). published material

Seeds were collected on October 26, 21 from a barn tree planted in a farmhouse in Gongju-si, Chungcheongnam-do. Germination experiments were carried out in four repetitions of 25 grains after injecting distilled water on washed river sand in a petri dish with a diameter of 9 cm.

Day length was given a photoperiod of 16:8 (L:D) using a fluorescent lamp (white cool) in the growth phase, and at this time, the light intensity was about 27 μmol·m ^-2 ·s ^-1 .

(2). Treatment and investigation method

In order to increase the germination rate of the seeds of Hovenia japonica, the germination rate was investigated after treatment in the following way. The germination rate was considered as germination when the radicle of the seed appeared more than 2 mm after the start of germination, and the number of germinated seeds was examined every week.

①. Seed coat undulation

The seed coat was broken with a chemical substance (sulfuric acid (H ₂ SO ₄ ) 98.0%) to increase the water absorption rate into the seed coat. Sulfuric acid (H ₂ SO ₄ ) (98.0%) was immersed for 0, 5, 10, 30, 60, 90, 120, and 180 minutes, then washed with distilled water, and the water absorption rate of the washed seeds was investigated based on the following equation.

%Ws=[(Wi-Wd)/Wd] × 100

In the above formula, Ws = increased seed weight, Wi = seed weight at elapsed time after water absorption, and Wd = initial weight.

② Alternative temperature treatment

The germination rate by alternating temperature was investigated in a growth chamber adjusted to 25/15 ℃, 20/10 ℃, 15/6 ℃, 5 ℃, respectively, based on untreated and sulfuric acid (H ₂ SO ₄ ) 60-minute treatment conditions.

③ Growth regulator treatment

After pre-cooling the seeds at 5° C. for 30 days, the growth regulator gibberellin (Gibberellin; GA ₃ ) was immersed at treatment concentrations of 0, 10, 100, and 1000 mg/L ^-1 for 24 hours, and then irradiated.

2. Experimental results

(One). Moisture Absorption Rate According to Hovenia Tree Sulfuric Acid (H ₂ SO ₄ ) Treatment Time

Figure 1 shows the water absorption rate according to the treatment time of barn tree sulfuric acid (H ₂ SO ₄ ).

As shown in FIG. 1, since the water absorption rate increased by about 100% or more after 60 minutes of treatment with sulfuric acid (H ₂ SO ₄ ), it can be seen that water absorption occurs only when sulfuric acid (H ₂ SO ₄ ) is treated for more than 60 minutes.

(2). Germination rate according to alternating temperature treatment

Figure 2 shows the germination rate according to alternating temperature treatment.

As shown in Figure 2, in the case of the control group showed a germination rate of 23.9% at 20/10 ℃, but the other treatments showed a low germination rate of less than 10%.

On the other hand, seeds in which moisture was absorbed into the seed coat through sulfuric acid (H ₂ SO ₄ ) treatment showed a high germination rate of 99.8% in the final germination rate, and germination appeared from 2 weeks at 25/15 ° C.

(3). Germination rate of growth regulator treatment

Gibberellin (GA ₃ ) When treated, about 50% of germination appeared in the highest treatment of 1000 ppm in general seeds, and it can be seen that the remaining treatments showed low germination rates without statistical significance.

In contrast, it can be seen that the seeds in which water was absorbed into the seed coat through sulfuric acid (H ₂ SO ₄ ) treatment showed a high germination rate of about 100% without any difference in the treatment section.

Table 1 below shows the final germination rate of hovenia seeds according to temperature conditions and hormone treatment.

division control treatment temperature condition 25/15℃ 9% 100% 20/10℃ 23.5% 100% 15/6℃ 4% 100% 5℃ 4% 100% hormone 0ppm 4% 100% 10 ppm 8% 100% 100 ppm 18% 100% 1000 ppm 50% 100%

Having described specific parts of the present invention in detail above, it will be clear to those skilled in the art that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby.

Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents. Simple modifications or changes of the present invention can be easily used by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.

Claims (9)

(a) immersing barnacle seeds in 98% (v/v) sulfuric acid (H ₂ SO ₄ ) for 60 minutes to break the seed coat;
(b) alternating temperature treatment by lowering the temperature of the corrugated seed coat from room temperature (25° C.) to low temperature (15° C.);
(c) pre-cooling the seed coat treated at 5° C. for 30 days; and
(d) treating the pre-cooled seed coat with 0.1 to 1000 ppm of gibberellin, a growth regulator; delete delete delete delete delete delete delete delete