CN107820771A - A kind of breeding method of apocarya rootstock seedling - Google Patents
A kind of breeding method of apocarya rootstock seedling Download PDFInfo
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- CN107820771A CN107820771A CN201710978597.0A CN201710978597A CN107820771A CN 107820771 A CN107820771 A CN 107820771A CN 201710978597 A CN201710978597 A CN 201710978597A CN 107820771 A CN107820771 A CN 107820771A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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Abstract
The invention belongs to the Cultivating techniques field of apocarya grafting rootstock seedling, and in particular to a kind of breeding method of apocarya rootstock seedling, including sterilize, soak seed, photo-irradiation treatment, treatment fluid handles and Pregermination and seedling breeding.The present invention has advantages below compared with prior art：The present invention handled with oxalic acid solution after with compound photo-irradiation treatment, can be easily broken walnut pericarp, then treatment fluid is soaked seed, vernalization, make the seed inside Walnut Fruit that there is higher physiologically active, whole operation process is simply controllable, make gained seedling regularity high, germination percentage is up to more than 99.2%, seedling ground diameter is 1.2 1.4cm, highly it is 80 100cm, after grafting, shorten apocarya cultivation period, survival rate is high after the walnut seedling grafting for being, growth rate is fast, the setting of plant ash and the disease resistance and resistance of nursery stock are favorably improved in pregermination procedure with chrysanthemum root aqueous extracts spray prior to seeding.
Description
Technical field
The invention belongs to the Cultivating techniques field of apocarya grafting rootstock seedling, and in particular to a kind of apocarya
The breeding method of rootstock seedling.
Background technology
Apocarya, also known as pecan, pecan tree, it is Juglandaceae hickory plant, originates in the U.S., Mo Xi
Brother, it is one of world-renowned dry fruit, fruit kernel percent is high, and the fragrant profit of food flavor is tasty and refreshing, and oil content is high, and shell is thin easy-peel；Trunk is logical straight,
Material is excellent, and because its tree body is tall and big, is thick with leaves, tree performance is graceful, is good township afforestation seeds again, its implantation methods ratio
More important, wherein especially more prominent with seed seedling-raising, seed seedling-raising is Seedling propagation, is directly to be cultivated using Walnut Seed
Go out walnut plant, thin shell mountain pecan peach seeds endotesta hard and compact, embryo can not absorb moisture in time and carry out aerobic metabolism, lead
Cause seed to sprout to be restricted, simultaneously because the mechanical resistance of kind skin hinders plumule and the growth of Baconic, broadcast using traditional
Kind method for culturing seedlings, thin shell mountain pecan peach seeds, which are sprouted, needs the 2-3 times of individual month, and germination and emergence is disappointing, then Seedling height growth
Measure and the rugosity of stock, therefore shortening are can be only achieved by 2-3 growth for 10-15cm, ground diameter 0.5cm or so, seedling
The time that thin shell mountain pecan peach seeds are sprouted, extend the growth period of nursery stock, improve the increment of nursery stock, be that research and production urgently solve
Key technology certainly, have been developed for promoting apocarya seed quick-speed germination and skill that is simple and using in the prior art
Art, the technical method are the physical resistances for first releasing thin shell mountain pecan peach seeds endotesta, and exogenous hormone GA3, IBA and KT can be direct
The cell division and growth of embryo radicle, plumule are acted on, under appropriate temperature conditions, seed is sprouted rapidly, germination percentage
Compared with prior art, the time of prolonged cold sand Tibetan is saved, avoids the mildew and rot risk of seed and seedling cost, but in reality
In operating process, the seed of breach carries out HORMONE TREATMENT, and regularity is not high after nursery, and germination percentage also needs further to improve.
The content of the invention
The purpose of the present invention is to be directed to the problem of existing, there is provided a kind of breeding method of apocarya rootstock seedling.
The present invention is achieved by the following technical solutions：A kind of breeding method of apocarya rootstock seedling, its feature
It is, including herein below：
（1）Ripe, complete apocarya seed disinfection is collected, is then placed in the oxalic acid solution that mass concentration is 0.6-0.8%
Middle immersion 8-12 hours, the temperature of the oxalic acid solution is 65-75 DEG C；
（2）It will be handled in apocarya seed photoenvironment after above-mentioned processing, treatment conditions are：Using LED monochrome feux rouges
620-660nm and blue light 455-485nm combinations are with quantity ratio 2:1 compound irradiation, the intensity of illumination are 650-850lux, processing
Time is 3-4 days, is stirred once every 12 hours in processing procedure；
（3）Thin shell mountain pecan peach seeds after above-mentioned processing are soaked in treatment fluid, soak 2-3 hours, the treatment fluid includes
The raw material of following parts by weight：100 parts of water, jasmonic 2.6-4.5 parts, ferrisodium salt 0.2-0.4 parts, ammonium nitrate 2-5 parts, yeast-β-
Glucan 1.4-1.8 parts；
（4）By the thin shell mountain pecan peach seeds after above-mentioned processing and sand by weight 2:After 3 mixing, phase is also included in the sand
When in its weight 6-8% plant ash, vernalization 8-10 days under conditions of temperature is 36-37 DEG C, humidity is 75-80 DEG C；
（5）Nursery in container for plant growth is moved into after seed with the spray germination of chrysanthemum root aqueous extracts.
As further improvement of these options, the thin shell mountain pecan peach seeds utilize the carbendazim of 600-800 times of liquid
Sterilization.
As further improvement of these options, the step（2）Photoenvironment be shading treatment device, it is described thin
Shell hickory nut lays in shading treatment device individual layer, and apocarya is 6-8cm apart from the distance of LED light source.
As further improvement of these options, relative humidity of the shading treatment device at work in device is
80-85%, temperature are 35-40 DEG C.
As further improvement of these options, the humidity of the sand is 45-50%.
As further improvement of these options, the preparation method of the chrysanthemum root aqueous extracts is by the chrysanthemum of collection
0.5-1cm segment is cut into after root system cleaning, is then soaked 48 hours with deionized water of 10 times equivalent to its weight, filtering,
Obtain chrysanthemum root aqueous extracts.
The present invention has advantages below compared with prior art：The present invention handled with oxalic acid solution after with compound photo-irradiation treatment,
It can be easily broken walnut pericarp, then treatment fluid seed soaking, vernalization, make the seed inside Walnut Fruit that there is higher physiology to live
Property, whole operation process is simply controllable, makes gained seedling regularity high, and germination percentage is up to more than 99.2%, and seedling ground diameter is 1.2-
1.4cm, be highly 80-100cm, after grafting, shorten apocarya cultivation period, after the walnut seedling grafting for being into
Motility rate is high, and growth rate is fast, the setting of plant ash and is helped prior to seeding with chrysanthemum root aqueous extracts spray in pregermination procedure
In the disease resistance and resistance that improve nursery stock.
Embodiment
Embodiment 1
A kind of breeding method of apocarya rootstock seedling, it is characterised in that including herein below：
（1）Ripe, complete apocarya seed disinfection is collected, is then placed in the oxalic acid solution that mass concentration is 0.7% and soaks
Bubble 10 hours, the temperature of the oxalic acid solution is 70 DEG C；
（2）It will be handled in apocarya seed photoenvironment after above-mentioned processing, treatment conditions are：Using LED monochrome feux rouges
640nm and blue light 475nm combinations are with quantity ratio 2:1 compound irradiation, the intensity of illumination are 750lux, and processing time is 3.5 days,
Stirred once every 12 hours in processing procedure；
（3）Thin shell mountain pecan peach seeds after above-mentioned processing are soaked in treatment fluid, soaked 2.5 hours, the treatment fluid includes
The raw material of following parts by weight：100 parts of water, 3.7 parts of jasmonic, 0.3 part of ferrisodium salt, 4 parts of ammonium nitrate, yeast-beta glucan 1.6
Part；
（4）By the thin shell mountain pecan peach seeds after above-mentioned processing and sand by weight 2:After 3 mixing, phase is also included in the sand
When in the plant ash of its weight 7%, the vernalization 9 days under conditions of temperature is 36.5 DEG C, humidity is 78 DEG C；
（5）Nursery in container for plant growth is moved into after seed with the spray germination of chrysanthemum root aqueous extracts.
Specifically, carbendazim sterilization of the thin shell mountain pecan peach seeds using 700 times of liquid；The step（2）Illumination ring
Border is shading treatment device, and the apocarya is laid in shading treatment device individual layer, and apocarya is apart from LED light source
Distance is 7m；The relative humidity in device is 82% to the shading treatment device at work, and temperature is 38 DEG C；The sand
Humidity is 48%.
Wherein, the preparation method of the chrysanthemum root aqueous extracts is to be cut into 0.8cm's after the chrysanthemum root system of collection is cleaned
Segment, then soaked 48 hours with deionized water of 10 times equivalent to its weight, filtering, obtain chrysanthemum root aqueous extracts.
Embodiment 2
A kind of breeding method of apocarya rootstock seedling, it is characterised in that including herein below：
（1）Ripe, complete apocarya seed disinfection is collected, is then placed in the oxalic acid solution that mass concentration is 0.6% and soaks
Bubble 12 hours, the temperature of the oxalic acid solution is 65 DEG C；
（2）It will be handled in apocarya seed photoenvironment after above-mentioned processing, treatment conditions are：Using LED monochrome feux rouges
660nm and blue light 455nm combinations are with quantity ratio 2:1 compound irradiation, the intensity of illumination are 850lux, and processing time is 3 days, place
Stirred once every 12 hours during reason；
（3）Thin shell mountain pecan peach seeds after above-mentioned processing are soaked in treatment fluid, soak 2 hours, the treatment fluid include with
The raw material of lower parts by weight：100 parts of water, 4.5 parts of jasmonic, 0.2 part of ferrisodium salt, 5 parts of ammonium nitrate, 1.4 parts of yeast-beta glucan；
（4）By the thin shell mountain pecan peach seeds after above-mentioned processing and sand by weight 2:After 3 mixing, phase is also included in the sand
When in the plant ash of its weight 8%, the vernalization 10 days under conditions of temperature is 36 DEG C, humidity is 80 DEG C；
（5）Nursery in container for plant growth is moved into after seed with the spray germination of chrysanthemum root aqueous extracts.
Specifically, carbendazim sterilization of the thin shell mountain pecan peach seeds using 800 times of liquid；The step（2）Illumination ring
Border is shading treatment device, and the apocarya is laid in shading treatment device individual layer, and apocarya is apart from LED light source
Distance is 6cm；The relative humidity in device is 85% to the shading treatment device at work, and temperature is 35 DEG C；The sand
Humidity is 50%.
Wherein, the preparation method of the chrysanthemum root aqueous extracts is to be cut into the small of 1cm after the chrysanthemum root system of collection is cleaned
Section, then soaked 48 hours with deionized water of 10 times equivalent to its weight, filtering, obtain chrysanthemum root aqueous extracts.
Embodiment 3
A kind of breeding method of apocarya rootstock seedling, it is characterised in that including herein below：
（1）Ripe, complete apocarya seed disinfection is collected, is then placed in the oxalic acid solution that mass concentration is 0.8% and soaks
Bubble 8 hours, the temperature of the oxalic acid solution is 75 DEG C；
（2）It will be handled in apocarya seed photoenvironment after above-mentioned processing, treatment conditions are：Using LED monochrome feux rouges
620nm and blue light 485nm combinations are with quantity ratio 2:1 compound irradiation, the intensity of illumination are 650lux, and processing time is 4 days, place
Stirred once every 12 hours during reason；
（3）Thin shell mountain pecan peach seeds after above-mentioned processing are soaked in treatment fluid, soak 3 hours, the treatment fluid include with
The raw material of lower parts by weight：100 parts of water, 2.6 parts of jasmonic, 0.4 part of ferrisodium salt, 2 parts of ammonium nitrate, 1.8 parts of yeast-beta glucan；
（4）By the thin shell mountain pecan peach seeds after above-mentioned processing and sand by weight 2:After 3 mixing, phase is also included in the sand
When in the plant ash of its weight 6%, the vernalization 8 days under conditions of temperature is 37 DEG C, humidity is 75 DEG C；
（5）Nursery in container for plant growth is moved into after seed with the spray germination of chrysanthemum root aqueous extracts.
Specifically, carbendazim sterilization of the thin shell mountain pecan peach seeds using 600 times of liquid；The step（2）Illumination ring
Border is shading treatment device, and the apocarya is laid in shading treatment device individual layer, and apocarya is apart from LED light source
Distance is 8cm；The relative humidity in device is 80% to the shading treatment device at work, and temperature is 40 DEG C；The sand
Humidity is 45%.
Wherein, the preparation method of the chrysanthemum root aqueous extracts is to be cut into 0.5cm's after the chrysanthemum root system of collection is cleaned
Segment, then soaked 48 hours with deionized water of 10 times equivalent to its weight, filtering, obtain chrysanthemum root aqueous extracts.
Control group 1 is set, the step 1 mesoxalic acid processing procedure of embodiment 1 removed, remaining content is constant；Control group is set
2, photo-irradiation treatment process in the step 2 of embodiment 1 is removed, remaining content is constant；Control group 3 is set, by the step 3 of embodiment 1
Jasmonic removes, and remaining content is constant；Control group 4 is set, yeast-beta glucan in the step 3 of embodiment 1 is removed, in remaining
Hold constant；Control group 5 is set, plant ash in the step 4 of embodiment 1 removed, remaining content is constant；Control group 6 is set, will be implemented
The seed step that germination is sprayed with chrysanthemum root aqueous extracts in example 1 removes, and remaining content is constant；Blank group is set, by existing side
Method nursery；
According to above method nursery, each group nursery quantity is 200 plants, No. 16 progress graftings of autumn August then after nursery, every group
Grafting quantity is 150 plants, observes and records thin-shelled walnut nursery and grafting situation, and grafting scion selection kind is excellent, bud is full
It is full, healthy and strong development branch is enriched, the wherein incidence of disease is black spot, anthracnose, root rot and weevil, the hair of chafer insect pest
Sick rate summation is as a result as follows：
Table 1
| Group | Germination percentage（%） | Ground diameter（cm） | Height of seedling（cm） | The incidence of disease（%） | Graft survival rate（%） |
| Embodiment 1 | 99.6 | 1.28 | 96.2 | 1.43 | 98.6 |
| Embodiment 2 | 99.7 | 1.26 | 98.4 | 1.27 | 98.2 |
| Embodiment 3 | 99.4 | 1.29 | 96.7 | 1.65 | 98.7 |
| Control group 1 | 72.5 | 0.94 | 94.8 | 2.04 | 96.3 |
| Control group 2 | 43.8 | 1.02 | 92.6 | 1.97 | 95.2 |
| Control group 3 | 93.2 | 0.86 | 80.7 | 2.15 | 94.8 |
| Control group 4 | 94.8 | 0.92 | 83.5 | 2.09 | 96.5 |
| Control group 5 | 98.5 | 1.15 | 87.6 | 5.27 | 97.3 |
| Control group 6 | 99.2 | 1.18 | 85.4 | 5.93 | 95.4 |
| Blank group | 92.5 | 1.13 | 81.5 | 5.76 | 90.2 |
Oxalic acid solution is can be seen that by data in table 1 and photo-irradiation treatment has a great influence to germination percentage, and treatment fluid composition is to stock
Seedling growing state has a great influence, in pregermination procedure the setting of plant ash and prior to seeding with chrysanthemum root aqueous extracts spray to plant
The disease resistance of strain has a great influence.
Claims (6)
1. a kind of breeding method of apocarya rootstock seedling, it is characterised in that including herein below：
（1）Ripe, complete apocarya seed disinfection is collected, is then placed in the oxalic acid solution that mass concentration is 0.6-0.8%
Middle immersion 8-12 hours, the temperature of the oxalic acid solution is 65-75 DEG C；
（2）It will be handled in apocarya seed photoenvironment after above-mentioned processing, treatment conditions are：Using LED monochrome feux rouges
620-660nm and blue light 455-485nm combinations are with quantity ratio 2:1 compound irradiation, the intensity of illumination are 650-850lux, processing
Time is 3-4 days, is stirred once every 12 hours in processing procedure；
（3）Thin shell mountain pecan peach seeds after above-mentioned processing are soaked in treatment fluid, soak 2-3 hours, the treatment fluid includes
The raw material of following parts by weight：100 parts of water, jasmonic 2.6-4.5 parts, ferrisodium salt 0.2-0.4 parts, ammonium nitrate 2-5 parts, yeast-β-
Glucan 1.4-1.8 parts；
（4）By the thin shell mountain pecan peach seeds after above-mentioned processing and sand by weight 2:After 3 mixing, phase is also included in the sand
When in its weight 6-8% plant ash, vernalization 8-10 days under conditions of temperature is 36-37 DEG C, humidity is 75-80 DEG C；
（5）Nursery in container for plant growth is moved into after seed with the spray germination of chrysanthemum root aqueous extracts.
A kind of 2. breeding method of apocarya rootstock seedling as claimed in claim 1, it is characterised in that the apocarya
Seed is sterilized using the carbendazim of 600-800 times of liquid.
A kind of 3. breeding method of apocarya rootstock seedling as claimed in claim 1, it is characterised in that the step（2）'s
Photoenvironment is shading treatment device, and the apocarya is laid in shading treatment device individual layer, apocarya distance LED
The distance of light source is 6-8cm.
A kind of 4. breeding method of apocarya rootstock seedling as claimed in claim 3, it is characterised in that the shading treatment dress
It is 80-85% to put the relative humidity at work in device, and temperature is 35-40 DEG C.
A kind of 5. breeding method of apocarya rootstock seedling as claimed in claim 1, it is characterised in that the humidity of the sand
For 45-50%.
A kind of 6. breeding method of apocarya rootstock seedling as claimed in claim 1, it is characterised in that the chrysanthemum root system water
The preparation method of immersion liquid is that 0.5-1cm segment is cut into after the chrysanthemum root system of collection is cleaned, then with equivalent to its weight 10
Deionized water again is soaked 48 hours, filtering, obtains chrysanthemum root aqueous extracts.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109121965A (en) * | 2018-08-23 | 2019-01-04 | 江苏绿苑园林建设有限公司 | A kind of seeding growing seedlings method of Acer palmatum |
| CN109757348A (en) * | 2019-02-26 | 2019-05-17 | 河北农业大学 | A method of the nursery of campanulaceae water planting is carried out with plant factor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856711A (en) * | 2017-02-08 | 2017-06-20 | 江苏省林业科学研究院 | A kind of method for promoting apocarya seed quick-speed germination |
-
2017
- 2017-10-19 CN CN201710978597.0A patent/CN107820771A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856711A (en) * | 2017-02-08 | 2017-06-20 | 江苏省林业科学研究院 | A kind of method for promoting apocarya seed quick-speed germination |
Non-Patent Citations (2)
| Title |
|---|
| 梁建萍: "《植物学》", 28 February 2014, 中国农业出版社 * |
| 王建林，关法春: "《高级作物生理学》", 31 March 2013, 中国农业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109121965A (en) * | 2018-08-23 | 2019-01-04 | 江苏绿苑园林建设有限公司 | A kind of seeding growing seedlings method of Acer palmatum |
| CN109757348A (en) * | 2019-02-26 | 2019-05-17 | 河北农业大学 | A method of the nursery of campanulaceae water planting is carried out with plant factor |
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