CN107109487B - 自动处理核酸和电泳样品制备的装置、方法和系统 - Google Patents

自动处理核酸和电泳样品制备的装置、方法和系统 Download PDF

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CN107109487B
CN107109487B CN201580067977.8A CN201580067977A CN107109487B CN 107109487 B CN107109487 B CN 107109487B CN 201580067977 A CN201580067977 A CN 201580067977A CN 107109487 B CN107109487 B CN 107109487B
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sample
reagent
gel
electrophoresis
hydrogel
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CN107109487A (zh
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E·S·阿布拉姆斯
D·尤恩
T·J·巴贝拉
D·G·萨宾
T·C·博尔斯
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Sage Science Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor

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  • Molecular Biology (AREA)
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  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
CN201580067977.8A 2014-10-15 2015-10-15 自动处理核酸和电泳样品制备的装置、方法和系统 Active CN107109487B (zh)

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Application Number Priority Date Filing Date Title
US201462064454P 2014-10-15 2014-10-15
US62/064,454 2014-10-15
US201562183514P 2015-06-23 2015-06-23
US62/183,514 2015-06-23
PCT/US2015/055833 WO2016061416A1 (en) 2014-10-15 2015-10-15 Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation

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CN107109487A CN107109487A (zh) 2017-08-29
CN107109487B true CN107109487B (zh) 2022-03-04

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US (2) US10131901B2 (enExample)
EP (1) EP3207163B1 (enExample)
JP (3) JP6612340B2 (enExample)
CN (1) CN107109487B (enExample)
CA (1) CA2964487C (enExample)
WO (1) WO2016061416A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2906935B1 (en) 2012-10-12 2018-04-04 Sage Science, Inc. Side-eluting molecular fractionator
EP4219687B1 (en) 2014-04-23 2024-08-14 Juno Therapeutics, Inc. Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy
WO2016061416A1 (en) 2014-10-15 2016-04-21 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
CA3005895A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
US10429387B2 (en) * 2016-07-27 2019-10-01 Universiteit Tweate Simple and affordable method for immuophenotyping using a microfluidic chip sample preparation with image cytometry
US10010883B2 (en) * 2016-09-20 2018-07-03 International Business Machines Corporation Deterministic lateral displacement arrays
EP3529400B1 (en) 2016-10-24 2021-02-17 Geneinfosec, Inc. Concealing information present within nucleic acids
AU2018250330A1 (en) 2017-04-07 2019-09-19 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification
CN108572099B (zh) * 2018-04-23 2021-03-26 上海交通大学 一种实现生物组织快速染色的方法和装置
WO2019237205A1 (en) * 2018-06-14 2019-12-19 Coastal Genomics Inc. Device for capturing macromolecules and methods for manufacturing and using same
SG11202101204TA (en) 2018-08-09 2021-03-30 Juno Therapeutics Inc Processes for generating engineered cells and compositions thereof
MX2021001519A (es) * 2018-08-09 2021-05-27 Juno Therapeutics Inc Metodos para valorar acidos nucleicos integrados.
WO2020112879A1 (en) * 2018-11-30 2020-06-04 Perkinelmer Health Sciences, Inc. Biological sample preparation using electric fields
CN109504676A (zh) * 2018-11-30 2019-03-22 厦门胜芨科技有限公司 一种dna大片段筛选回收试剂盒及其使用方法
WO2020185200A1 (en) 2019-03-08 2020-09-17 Hewlett-Packard Development Company, L.P. Disposable microfluidic cassettes
SG11202105834UA (en) * 2019-07-12 2021-07-29 Illumina Cambridge Ltd Nucleic acid library preparation using electrophoresis
US20230028621A1 (en) 2019-12-23 2023-01-26 Biopsense Oy Method, automated system and cartridge for extraction of cell-free nucleic acids from a blood sample
US20230087953A1 (en) 2020-02-12 2023-03-23 Juno Therapeutics, Inc. Bcma-directed chimeric antigen receptor t cell compositions and methods and uses thereof
CA3169672A1 (en) 2020-02-12 2021-08-19 Juno Therapeutics, Inc. Cd19-directed chimeric antigen receptor t cell compositions and methods and uses thereof
EP4139451A1 (en) * 2020-04-24 2023-03-01 Sage Science, Inc. Systems, devices, and methods for electrophoretic extracting and enriching extrachromosomal dna
US12292408B2 (en) * 2020-07-13 2025-05-06 Life Technologies Corporation Electrophoresis and electrotransfer devices, systems, and methods
USD975873S1 (en) 2020-07-13 2023-01-17 Life Technologies Corporation Electrophoresis and electrotransfer device
WO2022184836A1 (en) * 2021-03-03 2022-09-09 F. Hoffmann-La Roche Ag Devices and methods for electrophoretic extraction of nucleic acids from biological samples
CN112940915B (zh) * 2021-03-31 2025-07-04 四川工易生物科技有限公司 一种质粒提取装置及方法
WO2022226599A1 (en) * 2021-04-30 2022-11-03 University Of Tasmania Sample transfer method and system
CN116139950B (zh) * 2022-12-30 2025-11-04 江苏集萃医工交叉技术研究所有限公司 一种快速提取miRNA的微流控系统
TW202502363A (zh) 2023-02-28 2025-01-16 美商奇諾治療有限公司 用於治療全身性自體免疫疾病之細胞療法
CN116183707B (zh) * 2023-03-03 2024-05-24 吉林大学 一种快速比对分析生物样本的三维凝胶电泳装置和分离方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457050A (en) * 1993-11-30 1995-10-10 Board Of Regents, The University Of Texas System Agarose plug mold and processing chamber
CN102268426A (zh) * 2010-06-03 2011-12-07 中国科学院成都生物研究所 一种从环境样品中提取高分子量基因组dna的方法
CN102747427A (zh) * 2012-06-28 2012-10-24 中国农业科学院棉花研究所 利用非暗培养叶片构建棉花bac文库的方法
US8361299B2 (en) * 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
WO2014059188A1 (en) * 2012-10-12 2014-04-17 Sage Science, Inc. Side-eluting molecular fractionator

Family Cites Families (114)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3407133A (en) 1965-06-18 1968-10-22 Baxter Laboratories Inc Expendable electrophoresis apparatus
US3533933A (en) 1967-03-31 1970-10-13 Hannig Kurt Process and device for the isolation of fractions of a substance mixture electrophoretically separated in a carrier gel
US3616454A (en) 1969-03-13 1971-10-26 Univ New York Method of and apparatus for electrophoretic separation in a gel column
US3980546A (en) 1975-06-27 1976-09-14 Caccavo Francis A Micropreparative electrophoresis apparatus
DE2716095C2 (de) 1977-04-12 1987-02-19 Becton, Dickinson and Co., Paramus, N.J. Verfahren zur Steuerung der Bewegungsbahn von in einem Elektrolyten suspendierten Teilchen und Vorrichtung zur Durchführung dieses Verfahrens
US4315812A (en) 1980-05-28 1982-02-16 Karlson Eskil L Apparatus for continuous electrochromatographic separation
JPS57161638A (en) 1981-03-31 1982-10-05 Shimadzu Corp Method for atomic absorption analysis
US4375401A (en) 1981-05-20 1983-03-01 Nicholas Catsimpoolas Electrophoresis system
US4695548A (en) * 1982-11-18 1987-09-22 The Trustees Of Columbia University In The City Of New York Gel inserts useful in electrophoresis
FR2540122B1 (fr) 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
DE3337669C2 (de) 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
DE3337668C2 (de) 1983-10-17 1985-12-05 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Verfahren zur Elektroelution elektrisch geladener Makromoleküle
US4545888A (en) 1984-04-06 1985-10-08 Walsh J William Apparatus for electrophoretic recovery of nucleic acids and other substances
FR2568485B1 (fr) 1984-08-06 1990-03-23 Rhone Poulenc Rech Appareil de fractionnement par electrophorese de solutions contenant des proteines, utilisable notamment pour le fractionnement du plasma humain
US4707233A (en) 1985-04-29 1987-11-17 Gradiant Pty Limited Device for extraction of electrophoretic fractions
IL78703A (en) 1985-05-10 1993-02-21 Benzon Alfred Method of producing extracellular enzymes, the enzymes produced thereby and compositions containing them; a hybrid plasmid and a dna fragment comprising dna encoding the enzymes; a microorganism harbouring the plasmid; and a method for removing nucleic acids from biological materials
JPS62239047A (ja) 1986-04-11 1987-10-19 Hitachi Ltd 核酸塩基配列決定装置
US4708782A (en) 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
US4900677A (en) 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
EP0334615B1 (en) 1988-03-22 1994-11-17 Erik Nieman Electrophoresis apparatus
DE3829111A1 (de) 1988-08-27 1990-03-01 Hoechst Ag Verfahren und vorrichtung zum elektrophoretischen trennen, reinigen und anreichern von geladenen oder polarisierbaren makromolekuelen
US4834862A (en) 1988-09-12 1989-05-30 Duke University Ampholyte separation method and apparatus
US5169511A (en) 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
EP0382426A3 (en) 1989-02-06 1992-03-25 Applied Biosystems, Inc. Micro-preparative electrophoresis apparatus
US5062942A (en) 1989-04-12 1991-11-05 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
US6808609B1 (en) 1990-02-28 2004-10-26 Aclara Biosciences, Inc. Device and method for moving charged particles
US5217591A (en) 1990-05-14 1993-06-08 Labintelligence, Inc. Gel electrophoresis sample applicator/retriever
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5443704A (en) 1991-12-31 1995-08-22 Fmc Corporation Electrophoresis gel container assemblies
US5242568A (en) 1992-01-14 1993-09-07 Fotodyne Incorporated Electrophoresis apparatus
WO1993016788A1 (en) 1992-02-25 1993-09-02 Peter Andersen Process for electroelution of a gel containing separated charged macromolecules, such as proteins or dna/rna, and an apparatus and means for use in the process
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5433837A (en) 1993-08-27 1995-07-18 E. I. Du Pont De Nemours And Company Gel cassette for enhanced electrophoretic separation and processes for the preparation thereof
US5384022A (en) 1993-09-08 1995-01-24 Cornell Research Foundation, Inc. Method and apparatus for electrophoretic DNA band isolation
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US6306348B1 (en) 1993-11-01 2001-10-23 Nanogen, Inc. Inorganic permeation layer for micro-electric device
DE59410283D1 (de) 1993-11-11 2003-06-18 Aclara Biosciences Inc Vorrichtung und Verfahren zur elektrophoretischen Trennung von fluiden Substanzgemischen
US5705333A (en) 1994-08-05 1998-01-06 The Regents Of The University Of California Peptide-based nucleic acid mimics(PENAMS)
US5538614A (en) 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette
EP0805974A1 (en) 1995-01-23 1997-11-12 Anthony J. Murray Analysis of biological molecules
JP3581447B2 (ja) * 1995-08-22 2004-10-27 三菱電機株式会社 高耐圧半導体装置
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5801115A (en) 1995-09-05 1998-09-01 Kataleuna Gmbh Catalyst composition and methods for using and preparing same
US5717602A (en) 1996-02-05 1998-02-10 Kenning; Gregory G. Automated electrophoresis and analysis system
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
US5827418A (en) 1996-10-11 1998-10-27 Hoefer Pharmacia Biotech, Inc. Electrophoresis cassette
WO1999034205A1 (en) 1997-12-30 1999-07-08 Caliper Technologies Corp. Software for the display of chromatographic separation data
SE9800360D0 (sv) 1998-02-06 1998-02-06 Goeteborg University Science I Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules
US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
US20020049694A1 (en) 1998-07-27 2002-04-25 J. Wallace Parce Distributed database for analytical instruments
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
JP2000224980A (ja) 1999-02-05 2000-08-15 Matsushita Electric Ind Co Ltd 細菌濃縮装置
US6171466B1 (en) 1999-03-29 2001-01-09 Percy H. Rhodes Method and apparatus for electrophoretic focusing
US6290831B1 (en) 1999-08-30 2001-09-18 Integrated Genetic Devices, Ltd. Electrophoretic system for real time detection of multiple electrophoresed biopolymers
US6867851B2 (en) 1999-11-04 2005-03-15 Regents Of The University Of Minnesota Scanning of biological samples
DE10031028B4 (de) 2000-06-26 2008-09-04 Gnothis Holding Sa Verfahren zur Selektion von Partikeln
US6562213B1 (en) 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
DE60142520D1 (de) 2000-10-06 2010-08-19 Arturus Capital Ltd Multi-port-elektrophorese-trennvorrichtung und entsprechendes verfahren
CA2424941A1 (en) 2000-10-10 2002-04-18 Aviva Biosciences Corporation An integrated biochip system for sample preparation and analysis
EP1211510A1 (en) 2000-11-29 2002-06-05 Proteologics, Inc. Methods and apparatus for nonlinear mobility electrophoresis separation
AUPR222400A0 (en) 2000-12-21 2001-01-25 Life Therapeutics Limited Apparatus and method for separation of molecules and movementof fluids
JP2002310992A (ja) 2001-04-17 2002-10-23 Hitachi Electronics Eng Co Ltd マイクロチップおよびマイクロチップ電気泳動装置
JP4423810B2 (ja) 2001-04-27 2010-03-03 株式会社島津製作所 電気泳動装置
EP1384067B1 (en) 2001-05-02 2007-07-18 Applera Corporation Concentration and purification of analytes using electric fields
GB0128350D0 (en) 2001-11-27 2002-01-16 Lab901 Ltd Non-rigid apparatus for microfluidic applications
US7419784B2 (en) 2002-04-02 2008-09-02 Dubrow Robert S Methods, systems and apparatus for separation and isolation of one or more sample components of a sample biological material
FI20020746A7 (fi) 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
US6887668B2 (en) * 2002-04-19 2005-05-03 Beckman Coulter, Inc. Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye
US20070286773A1 (en) 2002-05-16 2007-12-13 Micronit Microfluidics B.V. Microfluidic Device
GB0215779D0 (en) 2002-07-08 2002-08-14 Deltadot Ltd Material separation device
US20040011650A1 (en) 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis
US6919571B2 (en) 2002-09-04 2005-07-19 Industrial Technology Research Institute Micro fluorescent electrophoresis detection system
DE60332046D1 (de) 2002-10-23 2010-05-20 Univ Princeton Verfahren zur kontinuierlichen teilchentrennung unter verwendungvon zu feldern asymmetrisch ausgerichteten hindernisarrays
US7108775B2 (en) 2002-11-08 2006-09-19 Applera Corporation Apparatus and method for confining eluted samples in electrophoresis systems
US7198703B2 (en) 2003-06-17 2007-04-03 Invitrogen Corporation Chamber-forming electrophoresis cassette cover
JP4254499B2 (ja) 2003-11-18 2009-04-15 株式会社島津製作所 質量分析用マイクロチップおよびこれを用いた質量分析装置
US7815783B2 (en) 2004-03-17 2010-10-19 Bio-Rad Laboratories, Inc. Multi-compartment filter and method of filtering using same
WO2005093388A1 (en) 2004-03-26 2005-10-06 Infectio Recherche Inc. Removable microfluidic flow cell
WO2006031385A2 (en) 2004-08-24 2006-03-23 The General Hospital Corporation Particle separating devices, systems and methods
US20060193752A1 (en) 2005-02-25 2006-08-31 Levine Leanna M Microvolume flowcell apparatus
US20060223178A1 (en) 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20070284250A1 (en) 2006-03-13 2007-12-13 Sage Science, Inc. Multifunctional Electrophoresis Cassette
KR100661482B1 (ko) * 2006-04-03 2006-12-27 전남대학교산학협력단 Dna 정제를 위한 수집홈을 갖는 전기영동장치
KR20090009302A (ko) 2006-05-03 2009-01-22 더 리전트 오브 더 유니버시티 오브 캘리포니아 단일 나노초승달 ｓｅｒｓ 탐침을 사용하는 프로테아제 및 프로테아제 활성의 검출
WO2008016414A2 (en) 2006-06-01 2008-02-07 The Trustees Of Princeton University Apparatus and method for continuous particle separation
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
BRPI0715346A2 (pt) 2006-08-01 2013-11-26 Applera Corp Detecção de analitos e ácidos nucléicos
EP2062036A4 (en) * 2006-08-31 2016-09-21 Life Technologies Israel Ltd METHODS, CASSETTES, GELS AND APPARATUS FOR ISOLATING AND COLLECTING BIOMOLECULES FROM ELECTROPHORESIS GELS
JP4862115B2 (ja) 2006-10-04 2012-01-25 国立大学法人北海道大学 マイクロチップおよびマイクロチップ電気泳動装置
CA2666019A1 (en) 2006-10-11 2008-04-24 Her Majesty The Queen In Right Of Canada, As Represented By The Ministerof The Province Of Nova Scotia, The Nova Scotia Agricultural College (Nsac) Proteins involved in after-cooking darkening in potatoes
WO2008131048A2 (en) 2007-04-16 2008-10-30 Cellpoint Diagnotics, Inc. Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells
DE102008020428B3 (de) 2008-04-24 2009-07-16 Johannes-Gutenberg-Universität Mainz Vorrichtung und Verfahren und Gelsystem zur analytischen und präparativen Elektrophorese
US8313906B2 (en) 2008-07-18 2012-11-20 Canon U.S. Life Sciences, Inc. Methods and systems for microfluidic DNA sample preparation
EP2304414B1 (en) 2008-07-24 2017-03-15 The Trustees of Princeton University Bump array device having asymmetric gaps for segregation of particles
JP5562342B2 (ja) * 2008-10-08 2014-07-30 セージ サイエンス， インコーポレイテッド 多重チャネルの分取用電気泳動システム
HUE027961T2 (en) 2008-10-24 2016-11-28 Epicentre Tech Corp Transposon final preparations and methods for modifying nucleic acids
WO2012171329A1 (zh) 2011-06-15 2012-12-20 Du Quan 一种核酸分离的方法及其应用
EP2739394A2 (en) 2011-08-04 2014-06-11 Sage Science, Inc. Microfluidic systems and methods for processing fluids
US10801017B2 (en) 2011-11-30 2020-10-13 The Broad Institute, Inc. Nucleotide-specific recognition sequences for designer TAL effectors
DE202013012597U1 (de) 2012-10-23 2017-11-21 Toolgen, Inc. Zusammensetzung zum Spalten einer Ziel-DNA, umfassend eine für die Ziel-DNA spezifische guide-RNA und eine Cas-Protein-codierende Nukleinsäure oder ein Cas-Protein, sowie deren Verwendung
GB201301164D0 (en) 2013-01-23 2013-03-06 Medical Res Council Methods for estimating the size of disease-associated polynucleotide repaet expansions in genes
WO2014186819A1 (en) 2013-05-20 2014-11-27 Nusep Holdings Limited Process for forming polyacrylamide membranes
WO2016061416A1 (en) 2014-10-15 2016-04-21 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US20170239658A1 (en) 2014-10-16 2017-08-24 Ezra S. Abrams Methods and systems for controlling the shear modulus of genomic length dsdna molecules
US11390908B2 (en) 2015-09-02 2022-07-19 University Of Massachusetts Detection of gene loci with CRISPR arrayed repeats and/or polychromatic single guide ribonucleic acids
CA3005895A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
WO2017139669A1 (en) 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
EP3523641A4 (en) 2016-10-04 2020-05-13 Sage Science, Inc. DEVICES, METHODS AND SYSTEMS FOR THE AUTOMATED PROCESSING OF NUCLEIC ACIDS AND ELECTROPHORETIC SAMPLE PREPARATION
AU2018250330A1 (en) 2017-04-07 2019-09-19 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457050A (en) * 1993-11-30 1995-10-10 Board Of Regents, The University Of Texas System Agarose plug mold and processing chamber
US8361299B2 (en) * 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
CN102268426A (zh) * 2010-06-03 2011-12-07 中国科学院成都生物研究所 一种从环境样品中提取高分子量基因组dna的方法
CN102747427A (zh) * 2012-06-28 2012-10-24 中国农业科学院棉花研究所 利用非暗培养叶片构建棉花bac文库的方法
WO2014059188A1 (en) * 2012-10-12 2014-04-17 Sage Science, Inc. Side-eluting molecular fractionator

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