CN107047543A - A kind of preparation method of resin embedding meat herbaceous plant sample - Google Patents
A kind of preparation method of resin embedding meat herbaceous plant sample Download PDFInfo
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- CN107047543A CN107047543A CN201710057103.5A CN201710057103A CN107047543A CN 107047543 A CN107047543 A CN 107047543A CN 201710057103 A CN201710057103 A CN 201710057103A CN 107047543 A CN107047543 A CN 107047543A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The present invention is a kind of preparation method of resin embedding meat herbaceous plant sample, is comprised the following steps：1）Conventional pretreatment is carried out to meat herbaceous plant；2）Guarantor's color solution of different colours is configured, meat herbaceous plant is placed therein carry out impregnation process by color classification；3）The meat herbaceous plant handled well is taken out, rinsed with distilled water；4）The organic solution of various concentrations is configured, meat herbaceous plant is placed therein the organic replacement Treatment of progress；5）The meat herbaceous plant handled well is taken out, the solution on surface is removed；6）The above-mentioned meat herbaceous plant handled well is placed in silica gel mould, embedded with resin；7）After after hardening of resin, produce.Using the present invention, the herbal resin sample of meat using meat herbaceous plant as representative can be made, the sample being embedded in resin can keep primary color and form.The present invention can overcome the disadvantages that the vacancy of meat herbaceous plant preparation of specimen, more truly intuitively show phytomorph.
Description
Technical field
The present invention relates to plant specimen preparation method, the preparation method for more particularly relating to meat herbaceous plant sample.
Background technology
Making the plant specimen used in current scientific research institution mainly has herbarium, dipping specimen.Herbarium is also known as
Squeeze preparation, is the sample being made up of steps such as shaping-compacting-bookbinding-preservations, but the organ such as plant flowers and fruits be crushed it is viscous
Together, it is difficult to dissect and observe.And change colour because being easy to aoxidize for a long time in air, it is impossible to it is long-term to preserve.Meat grass
This plant is difficult to be preserved with the preparation method of herbarium because of its cauline leaf solid.Dipping specimen is plant through chemicals
Immersion keeps the sample of original form, but the frangible and full liquid heavy greatly of specimen bottle volume used in anti-corrosive properties dipping specimen,
Not readily transportable or carrying.Though in addition, the method for resin embedding plant specimen has been proposed, many methods using drying are handled
A kind of teaching preparation method of resin sample that plant, such as Chinese patent CN105900977A provide, uses drier pair
Plant is carried out after encapsulation dehydration, is reused resin and is embedded；But this method is not suitable for the plant of all kinds, esp meat
Matter herbaceous plant, if carrying out processing meat herbaceous plant using seasoning, its loose meat cauline leaf, which morphologically occurs, to wither
Compression deformation etc. is larger to be changed.In addition, Chinese patent CN105010302A provides a kind of system of the aqueous zoological specimens of resin embedding
Make method, method carries out preservative treatment with impregnating fluid to aqueous zoological specimens；But this method can not keep the color of plant.Total
From the point of view of, current meat herbaceous plant preparation of specimen technology relatively lacks.
The content of the invention
It is an object of the invention to provide a kind of preparation method of resin embedding meat herbaceous plant sample, it is intended to solves existing
There is the problem of plant specimen preparation method is difficult to preserve meat herbaceous plant and not good meat herbaceous plant sample effect.
To realize purpose, the present invention is a kind of preparation method of resin embedding meat herbaceous plant sample, including following step
Suddenly：
1) conventional pretreatment is carried out to meat herbaceous plant；
2) guarantor's color solution of different colours is configured, meat herbaceous plant is placed therein at progress dipping by color classification
Reason；
3) the meat herbaceous plant handled well is taken out, rinsed with distilled water；
4) organic solution of various concentrations is configured, meat herbaceous plant is placed therein the organic replacement Treatment of progress；
5) the meat herbaceous plant handled well is taken out, removes the solution on surface；
6) the above-mentioned meat herbaceous plant handled well is placed in silica gel mould, embedded with resin；
7) after after hardening of resin, producing meat herbaceous plant resin sample.
Further, in step 1) in, it is described that the conventional pretreatment of meat herbaceous plant progress is referred to, by the meat of collection
Herbaceous plant removes silt impurity and deadwood and rotten leaf, such as removes the dust on surface using hairbrush or elutes plant with clear water.For
The fine hair on meat herbaceous plant surface, it should be noted that retain.
Further, in step 2) in, the common configuration method of described guarantor's color solution for different colours sample is as follows
Table：
| Green sample | The formalin of 10% copper sulphate 0.5% |
| Yellow and light green color sample | 0.15%~0.5% sulfurous acid |
| Red sample | The formalin of 1% boric acid 1% |
| Bluish violet sample | 2% aluminum sulfate solution |
Further, in step 2) in, meat herbaceous plant sorting placement is subjected to impregnation process in guarantor's color solution, wherein,
Plant need to be totally submerged in solution, the plant of collection can be uniformly put into non-woven bag, is pressed into the lump in solution；Its
In, dip time is 12-24 hours, and specific time span can be depending on the carnification degree according to plant stem-leaf.Wherein, blade compared with
Thick plant can extend dip time, to ensure color effect.
Further, in step 3) in, the meat herbaceous plant handled well is rinsed with distilled water, it is residual that rinsing removes plant surface
Remaining guarantor's color solution.Rinsing time can not be long, is separated out to prevent protecting color solution.
Further, in step 4) in, the organic solution of described various concentrations is molten for the concentration gradient of ethanol and dimethylbenzene
Liquid.Such as：The ethanol of the ethanol solution of 70% ethanol solution → 80% → 90% ethanol solution → absolute ethyl alcohol → absolute ethyl alcohol → 50%
The diformazan of the xylene solution of the xylene solution of solution+50% → xylene solution of 25% ethanol solution+75% → 100% → 100%
Benzole soln；
Meat herbaceous plant is positioned over organic solution and carries out organic replacement Treatment, soak time is in every kind of solution
20-30 minutes.
Further, in step 5) in, the solution for handling plant surface well is removed, can be using method of drying or blower fan drying method.
Further, in step 6) in, the resin is by epoxy resin and curing agent in mass ratio 2.9：1 uniform stirring is mixed
After conjunction, stand 10 minutes bubbles and eliminate obtained resin naturally.
Further, in step 6) in, the above-mentioned meat herbaceous plant handled well is placed in silica gel mould, carried out with resin
Embedding, specific method is as follows：
A, appropriate epoxy resin is injected into silica gel mould, the plant after processing is put into；
B, after first resin by injection 6-10 hours, epoxy resin is re-injected, plant is embedded completely；
Further, in step 7) in, at normal temperatures, the hardening of resin time is 12-24 hours.
Relative to prior art, beneficial effects of the present invention are：
Using the technical method of the present invention, the meat herbaceous plant form color being embedded in resin is true to nature, the mark of making
This transparency is high, it is easy to which the details of observation of plant all directions, sample shape size is various, light to be readily transported and carry, and
Resin does not turn yellow for a long time, and sample is anti-oxidant being capable of long Kubo color.Existing plant specimen preparation method is solved to be difficult to preserve meat
The problem of matter herbaceous plant.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing skill
The accompanying drawing used required in art description is briefly described, it should be apparent that, drawings in the following description are only the present invention
Some embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, can also basis
These accompanying drawings obtain other accompanying drawings.
Fig. 1 illustrates for a kind of preparation method flow of the meat herbaceous plant sample of resin embedding provided in an embodiment of the present invention
Figure.
Embodiment
Experimentation is as follows：Herborization sample, carries out conventional pretreatment.A variety of salting liquids are configured as guarantor's color of plant
Solution, by different types of plant according to its color classification, is soaked in guarantor's color solution corresponding to its primary color respectively, protects
Hold its primary color.Using organic solvents such as ethanol, dimethylbenzene, the maceration extract of various concentrations is configured to, plant is soaked in it
In, plant cell liquor is progressively entered line replacement with organic solvent, finally make to fill dimethylbenzene in plant cell, keep its saturation degree
And glossiness.Root is it was found that obtain epoxy resin and curing agent is most adapted to ratio, according to being most adapted to than configuring obtained asphalt mixtures modified by epoxy resin
Fat transparency highest, hardness are most suitable and rate of heat release does not influence the state of plant.Will be through above-mentioned with the epoxy resin configured
The plant of processing is soaked in wherein and adjusts form and the position of plant, and epoxy resin is placed in normal temperature and solidified, final
To new type resin sample model.By the new type resin preparation of specimen Technology application in meat herbaceous plant, primary color has been obtained
And form preserves good meat herbaceous plant sample, compensate for the vacancy of meat herbaceous plant preparation of specimen.
Further illustrated with reference to specific embodiment：
Example one：
A kind of making of the blue stone lotus sample of resin embedding comprises the following steps：
1) blue stone lotus plant is cleaned, removes its deadwood and rotten leaf, cut unnecessary rhizome, it is carried out clearly with distilled water
Wash, treat that it is dried；
2) guarantor's color solution of configuration sulfur acid aluminium 2%, blue stone lotus plant is installed with non-woven bag and is placed in one after tightening
The impregnation process of a length of 15 hours during progress；
3) non-woven bag is taken out with tweezers, opens sack, the keeping color agent on plant surface is washed away with distilled water, its nature is treated
After air-drying or being dried up with blower fan, non-woven bag is put into organic displacement liquid；
4) by blue stone lotus plant successively in 70% ethanol solution, 80% ethanol solution, 90% ethanol solution, 95% ethanol is molten
Liquid, absolute ethyl alcohol, absolute ethyl alcohol, the xylene solution of 50% ethanol solution+50%, the xylene solution of 25% ethanol solution+75%,
30min is soaked in 100% xylene solution, 100% xylene solution；
5) the blue stone lotus plant handled well is taken out, the method dried up with natural air drying or blower fan removes the molten of surface
Liquid；
6) configuration epoxy resin and curing agent mass ratio are 2.9:1 resin, stir 2 minutes, stand 10 minutes it is to be mixed
After liquid bubble dissipates naturally, the appropriately sized silica gel mould for containing plant is taken out, by a little resin uniform fold in mold bottom,
The above-mentioned blue stone lotus plant handled well is placed in mould, then adds resin to just submerging at plant, resin is treated after 6-7 hours
When slightly admittedly, add resin and be covered with mould to whole；
7) solidified completely after resin after 24 hours, produce blue stone lotus propolis sample.
Obtained blue stone lotus resin plant sample in the method, its color is almost consistent with its growth conditions, Plant Leaf
Piece is unfolded, and plant surface occurs without white fog and mould, without floccule and impurity inside resin, and transparency is high, it is easy to
Observation is appreciated；The sample is placed in natural environment 2 months, resin aging is not found, the phenomenon such as plant rots.
Example two：
The making that a kind of brocade of resin embedding shakes asterisk sheet comprises the following steps：
1) shake star plant to brocade to handle, remove its deadwood and rotten leaf, cut unnecessary rhizome, its surface ash is brushed away with brush
Trifle in the world matter, it is standby；
2) guarantor's color solution of the formalin Han 10% copper sulphate 0.5% is configured, brocade is shaken into star plant is installed with non-woven bag
Be placed in one the impregnation process of a length of 24 hours when carrying out after tightening；
3) non-woven bag is taken out with tweezers, opens sack, the keeping color agent on plant surface is washed away with distilled water, its nature is treated
After air-drying or being dried up with blower fan, non-woven bag is put into organic displacement liquid；
4) brocade is shaken into star plant successively in 70% ethanol solution, 80% ethanol solution, 90% ethanol solution, 95% ethanol is molten
Liquid, absolute ethyl alcohol, absolute ethyl alcohol, the xylene solution of 50% ethanol solution+50%, the xylene solution of 25% ethanol solution+75%,
30min is soaked in 100% xylene solution, 100% xylene solution；
5) brocade handled well is shaken into star plant to take out, the method dried up with natural air drying or blower fan removes the molten of surface
Liquid；
6) configuration epoxy resin and curing agent mass ratio are 2.9:1 resin, stir 2 minutes, stand 10 minutes it is to be mixed
After liquid bubble dissipates naturally, the appropriately sized silica gel mould for containing plant is taken out, by a little resin uniform fold in mold bottom,
The above-mentioned brocade handled well is shaken star plant to be placed in mould, then adds resin to just submerging at plant, resin is treated after 6-7 hours
When slightly admittedly, add resin and be covered with mould to whole；
7) solidified completely after resin after 24 hours, produce brocade and shake star propolis sample.
Obtained blue stone lotus resin plant sample in the method, its color is almost consistent with its growth conditions, Plant Leaf
Piece is full, and plant surface occurs without white fog and mould, without floccule and impurity inside resin, and transparency is high, with putting
Big mirror can clearly see that brocade shakes the fine hair posture feature on star plant surface, it is easy to which observation is appreciated；The sample is placed in nature ring
2 months in border, resin aging is not found, the phenomenon such as plant rots.Example three：
A kind of making of the beautiful samples of the Ji Qiu of resin embedding comprises the following steps：
1) the beautiful plant of Ji Qiu are cleaned, removes its deadwood and rotten leaf, cut unnecessary rhizome, it is carried out clearly with distilled water
Wash, treat that it is dried；
2) guarantor color solution of the configuration containing 0.15%~0.5% sulfurous acid, the beautiful plant of Ji Qiu are installed with non-woven bag and tightened
Be placed in one the impregnation process of a length of 20 hours when carrying out afterwards；
3) non-woven bag is taken out with tweezers, opens sack, the keeping color agent on plant surface is washed away with distilled water, its nature is treated
After air-drying or being dried up with blower fan, non-woven bag is put into organic displacement liquid；
4) by the beautiful plant of Ji Qiu successively in 70% ethanol solution, 80% ethanol solution, 90% ethanol solution, 95% ethanol is molten
Liquid, absolute ethyl alcohol, absolute ethyl alcohol, the xylene solution of 50% ethanol solution+50%, the xylene solution of 25% ethanol solution+75%,
30min is soaked in 100% xylene solution, 100% xylene solution；
5) the beautiful plant of the Ji Qiu handled well are taken out, the method dried up with natural air drying or blower fan removes the molten of surface
Liquid；
6) configuration epoxy resin and curing agent mass ratio are 2.9:1 resin, stir 2 minutes, stand 10 minutes it is to be mixed
After liquid bubble dissipates naturally, the appropriately sized silica gel mould for containing plant is taken out, by a little resin uniform fold in mold bottom,
The above-mentioned beautiful plant of the Ji Qiu handled well are placed in mould, then add resin to just submerging at plant, resin is treated after 6-7 hours
When slightly admittedly, add resin and be covered with mould to whole；
7) solidified completely after resin after 24 hours, produce the beautiful propolis samples of Ji Qiu.
Beautiful resin plant samples of obtained Ji Qiu in the method, its color is almost consistent with its growth conditions, plant appearance
State is stood upright, and plant surface occurs without white fog and mould, without floccule and impurity inside resin, and transparency is high, it is easy to
Observation is appreciated；The sample is placed in natural environment 2 months, resin aging is not found, the phenomenon such as plant rots.
Example four：
A kind of making of the jade sample of the rainbow of resin embedding comprises the following steps：
1) the jade plant of rainbow is cleaned, removes its deadwood and rotten leaf, cut unnecessary rhizome, it is carried out clearly with distilled water
Wash, treat that it is dried；
2) guarantor color solution of the configuration containing 1% boric acid and 1% formalin, the jade plant of rainbow is installed with non-woven bag and tightened
Be placed in one the impregnation process of a length of 20 hours when carrying out afterwards；
3) non-woven bag is taken out with tweezers, opens sack, the keeping color agent on plant surface is washed away with distilled water, its nature is treated
After air-drying or being dried up with blower fan, non-woven bag is put into organic displacement liquid；
4) by the jade plant of rainbow successively in 70% ethanol solution, 80% ethanol solution, 90% ethanol solution, 95% ethanol is molten
Liquid, absolute ethyl alcohol, absolute ethyl alcohol, the xylene solution of 50% ethanol solution+50%, the xylene solution of 25% ethanol solution+75%,
25min is soaked in 100% xylene solution, 100% xylene solution；
5) the jade plant for the rainbow handled well is taken out, the method dried up with natural air drying or blower fan removes the molten of surface
Liquid；
6) configuration epoxy resin and curing agent mass ratio are 2.9:1 resin, stir 2 minutes, stand 10 minutes it is to be mixed
After liquid bubble dissipates naturally, the appropriately sized silica gel mould for containing plant is taken out, by a little resin uniform fold in mold bottom,
The jade plant of the above-mentioned rainbow handled well is placed in mould, then adds resin to just submerging at plant, resin is treated after 6-7 hours
When slightly admittedly, add resin and be covered with mould to whole；
7) solidified completely after resin after 24 hours, produce the jade propolis sample of rainbow.
The cajaput fat plant specimen of obtained rainbow in the method, its color is almost consistent with its growth conditions, plant appearance
State is stood upright, and plant surface occurs without white fog and mould, without floccule and impurity inside resin, and transparency is high, it is easy to
Observation is appreciated；The sample is placed in natural environment 2 months, resin aging is not found, the phenomenon such as plant rots.
As known from the above examples, above example is to combine the further explaination that implementation steps are made to the present invention, and this is simultaneously
All specific implementation examples of the present invention, premise of the those skilled in the art in the design for not departing from the present invention can not be forgiven completely
Under, the change and replacement made to the present invention are belonged within the claims in the present invention and equivalent technologies scope, should all be belonged to
In protection scope of the present invention.
Claims (10)
1. a kind of preparation method of resin embedding meat herbaceous plant sample, it is characterised in that comprise the following steps：
1) conventional pretreatment is carried out to meat herbaceous plant；
2) guarantor's color solution of different colours is configured, meat herbaceous plant is placed therein carry out impregnation process by color classification；
3) the meat herbaceous plant handled well is taken out, rinsed with distilled water；
4) organic solution of various concentrations is configured, meat herbaceous plant is placed therein the organic replacement Treatment of progress；
5) the meat herbaceous plant handled well is taken out, removes the solution on surface；
6) the above-mentioned meat herbaceous plant handled well is placed in silica gel mould, embedded with resin；
7) after after hardening of resin, producing meat herbaceous plant resin sample.
2. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 1) in, conventional pretreatment is carried out to meat herbaceous plant and is referred to, the meat herbaceous plant of collection is removed into silt impurity and withered
Branch rotten leaf, such as removes the dust on surface using hairbrush or elutes plant with clear water., should for the fine hair on meat herbaceous plant surface
Note retaining.
3. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 2) in, for different colours sample guarantor color solution common configuration method such as following table：
。
4. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 2) in, by meat herbaceous plant sorting placement in protect color solution carry out impregnation process, wherein, plant need to be totally submerged in molten
In liquid, the plant of collection can be uniformly put into non-woven bag, be pressed into the lump in solution；Wherein, dip time is that 12-24 is small
When, specific time span can be depending on the carnification degree according to plant stem-leaf.Wherein, when the thicker plant of blade can extend dipping
Between, to ensure color effect.
5. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 3) in, rinse the meat herbaceous plant handled well with distilled water, rinsing removes plant surface remnants and protects color solution.Rinsing
Time can not be long, is separated out to prevent protecting color solution.
6. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 4) in, the organic solution of various concentrations is ethanol and the concentration gradient solution of dimethylbenzene.Such as：
The ethanol solution of 70% ethanol solution → 80% → 90% ethanol solution → absolute ethyl alcohol → absolute ethyl alcohol → 50% ethanol is molten
The dimethylbenzene of the xylene solution of the xylene solution of liquid+50% → xylene solution of 25% ethanol solution+75% → 100% → 100%
Solution；
Meat herbaceous plant is positioned over organic solution and carries out organic replacement Treatment, soak time is 20-30 in every kind of solution
Minute.
7. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 5) in, remove and handle the solution of plant surface well, method is dried up using the method for drying or blower fan.
8. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 6) in, the resin is by epoxy resin and curing agent in mass ratio 2.9：After the mixing of 1 uniform stirring, 10 minutes bubbles are stood
Naturally obtained resin is eliminated.
9. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that in step
It is rapid 6) in, the above-mentioned meat herbaceous plant handled well is placed in silica gel mould, embedded with resin, specific method is as follows：
A, appropriate epoxy resin is injected into silica gel mould, the plant after processing is put into；
B, after first resin by injection 6-10 hours, epoxy resin is re-injected, plant is embedded completely.
10. a kind of preparation method of resin embedding meat herbaceous plant sample as claimed in claim 1, it is characterised in that
Step 7) in, at normal temperatures, the hardening of resin time is 12-24 hours.
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| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN111345287A (en) * | 2019-12-31 | 2020-06-30 | 江西农业大学 | Kiwi fruit plant tissue solidified colloid specimen and manufacturing method thereof |
| CN111572937A (en) * | 2020-05-07 | 2020-08-25 | 重庆市中药研究院 | Novel wine bottle processing and manufacturing method based on embedding technology |
| CN111869568A (en) * | 2020-08-17 | 2020-11-03 | 上海应用技术大学 | Hippeastrum rutilum packaging product chemical structure and manufacturing method thereof |
| CN112640893A (en) * | 2020-12-16 | 2021-04-13 | 赣南树木园 | Manufacturing method of sightseeing wood color-preserving specimen |
| CN115399310A (en) * | 2022-08-23 | 2022-11-29 | 贵州大学 | Manufacturing method and application of epoxy resin embedded animal tissue specimen |
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| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN108990968A (en) * | 2018-09-25 | 2018-12-14 | 河南科技大学 | A kind of production method of the biological plasticized sample of Alfalfa primary colors overall picture |
| CN111345287A (en) * | 2019-12-31 | 2020-06-30 | 江西农业大学 | Kiwi fruit plant tissue solidified colloid specimen and manufacturing method thereof |
| CN111572937A (en) * | 2020-05-07 | 2020-08-25 | 重庆市中药研究院 | Novel wine bottle processing and manufacturing method based on embedding technology |
| CN111869568A (en) * | 2020-08-17 | 2020-11-03 | 上海应用技术大学 | Hippeastrum rutilum packaging product chemical structure and manufacturing method thereof |
| CN112640893A (en) * | 2020-12-16 | 2021-04-13 | 赣南树木园 | Manufacturing method of sightseeing wood color-preserving specimen |
| CN112640893B (en) * | 2020-12-16 | 2022-08-19 | 赣南树木园 | Manufacturing method of sightseeing wood color-preserving specimen |
| CN115399310A (en) * | 2022-08-23 | 2022-11-29 | 贵州大学 | Manufacturing method and application of epoxy resin embedded animal tissue specimen |
| CN115399310B (en) * | 2022-08-23 | 2024-01-16 | 贵州大学 | Manufacturing method and application of epoxy resin embedded animal tissue specimen |
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Application publication date: 20170818 |