CN107041369A - A kind of bactericidal composition for preventing and treating the late blight of potato - Google Patents
A kind of bactericidal composition for preventing and treating the late blight of potato Download PDFInfo
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- CN107041369A CN107041369A CN201710247195.3A CN201710247195A CN107041369A CN 107041369 A CN107041369 A CN 107041369A CN 201710247195 A CN201710247195 A CN 201710247195A CN 107041369 A CN107041369 A CN 107041369A
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- Prior art keywords
- chinese yam
- boscalid
- potato
- bactericidal composition
- yam polysaccharide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The present invention relates to a kind of bactericidal composition for preventing and treating the late blight of potato, active ingredient is made up of Chinese yam polysaccharide and Boscalid, and the weight ratio of Chinese yam polysaccharide and Boscalid is 30:1‑1:15.The bactericidal composition that the present invention carries out rational mixture by Chinese yam polysaccharide and Boscalid is used for the preventing and treating of the late blight of potato, has significant synergies, and reduces Pesticide use amount, reduces cost accounting, environmentally friendly to crop safety.
Description
Technical field
The present invention relates to pesticides application technical field, and in particular to a kind of bactericidal composition for preventing and treating the late blight of potato.
Background technology
Potato (scientific name：Solanum tuberosum L.), belong to Solanaceae herbaceos perennial, stem tuber can be edible,
It is the fourth-largest important cereal crops in the whole world, is only second to wheat, paddy and corn.There are China, Russia sieve in potato major producing country
This, India, Ukraine, U.S. etc..China is the most country of world's potato total yield.2015, China will start potato
Staple food grainization strategy, promotes and potato is processed into the staple foods such as steamed bun, noodles, ground rice, and potato will be into outside rice, wheat, corn
Another staple food grain.The late blight of potato (Potato Late Blight) is caused by phytophthora infestans, causes potato haulm dead
A kind of crushing oomycetes disease rotted with stem tuber.All over the world, there is generation in potato producing region to the late blight of potato, popular
The year general underproduction 30%.More serious, northeast, North China and northwest rainy moist time in the regional potato place of production of Southwestern China
Cause harm heavier.
Chinese yam (Dioscorea opposita Thunb) also known as Chinese yam, prolong chapter, jade and prolong, be Dioscoreaceae Dioscorea for many years
The dry tuber of raw perennial root gadding plant.Modern medicine study thinks that the pharmacological activity main component of Chinese yam is Chinese yam polysaccharide (Yam
polysaccharide).Have much to the research of Chinese yam polysaccharide both at home and abroad at present, have been reported that Chinese yam polysaccharide have immunological regulation,
Antitumor, anti-aging, anti-mutation, anti-oxidant etc. are acted on.But the antibacterial action on Chinese yam polysaccharide studies still few, Yu Lian, Zhang Jun
It is graceful, Ma Shuxia, Zhang Tao, what Meng Dexin was delivered《Chinese yam polysaccharide optimal extraction technology and its antibacterial activity research》Confirm Chinese yam polysaccharide
There is certain fungistatic effect to staphylococcus aureus, Escherichia coli, Candida albicans etc..
Boscalid, English common name boscalid, chemical name：The chloro- N- of 2- (4 '-chlorodiphenyl -2- bases) niacinamide.Pyridine
Acyl bacterium amine is Novel tobacco acid amide fungicides, and fungicidal spectrum is wider, almost all active to all types of fungal diseases, to preventing and treating
Powdery mildew, gray mold, sclerotiniose and various rots etc. are highly effective, and also effective to the resistance bacterium of other medicaments, mainly
Preventing and treating for including the diseases such as rape, grape, fruit tree, vegetables and field crop.
Whether Boscalid or other bactericide are used alone for a long time, easily disease is developed immunity to drugs, cause
The problem of dosage is increased, preventive effect is reduced.Be reduction resistance risk and delaying drug resistance produce, by the different mechanisms of action it is effective into
Point compounded, using being one of effective method.
At present the research of the late blight of potato is applied to using Chinese yam polysaccharide, Boscalid as the composition of active ingredient not yet
There is relevant report.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of bactericidal composition for preventing and treating the late blight of potato.
The present invention is to be achieved through the following technical solutions：A kind of bactericidal composition for preventing and treating the late blight of potato, effectively into
Divide and be made up of Chinese yam polysaccharide and Boscalid, the weight ratio of Chinese yam polysaccharide and Boscalid is 30:1-1:15.Preferably, Chinese yam
The weight ratio of polysaccharide and Boscalid is 20:1-1:10.
Described bactericidal composition active ingredient accounts for the 1-70% of bactericidal composition gross weight.
Described bactericidal composition adds the agent that corresponding adjuvant is made according to method known in those skilled in the art
Type is water dispersible granules or suspending agent.
The adjuvant that bactericidal composition of the present invention is used includes dispersant, wetting agent, antifreezing agent, binding agent, disintegrant, disappeared
Infusion, thickener, carrier etc. or it is other be beneficial to active ingredient in the formulation stablize and drug effect play known substance, be all agriculture
The various composition used is commonly used or allowed in medicine formulation art.
The dispersant and wetting agent be selected from calcium dodecyl benzene sulfonate, alkyl benzene sulfonate calcium salt, lauryl sodium sulfate,
Polycarboxylate, lignosulfonates, APES, sodium hydroxyethyl cellulose, Negel, fatty alcohol polyoxy
Ethene ether phosphate, AEO, sodium butylnaphthalenesulfonate, styrylphenol polyoxyethylene ether, tea seed cake, gleditsia sinensis
One or more in powder, silkworm excrement, spaonin powder, sldium lauryl sulfate, pull open powder.
The defoamer is selected from organosilicon.
The one kind of the antifreezing agent in ethylene glycol, propane diols, glycerine, isopropanol.
The one kind of the thickener in xanthans, Arabic gum, Magnesiumaluminumsilicate, carboxymethyl cellulose.
The one kind of the binding agent in starch, polyvinyl alcohol, ethyl cellulose.
The one kind of the disintegrant in sodium carbonate, calcium chloride, ammonium carbonate, potassium chloride, sodium sulphate.
The carrier is in kaolin, attapulgite, soil powder, diatomite, bentonite, white carbon, deionized water
It is one or more.
Beneficial effect of the present invention：The bactericidal composition that the present invention carries out rational mixture by Chinese yam polysaccharide and Boscalid is used
In the preventing and treating of the late blight of potato, have significant synergies, reduce Pesticide use amount, reduce cost accounting, crop is pacified
Entirely, it is environmentally friendly.
Embodiment
Technical scheme and technique effect are described further below in conjunction with specific embodiment, institute of the present invention
The percentage stated is weight percentage.
Implementation of an application example one：Chinese yam polysaccharide and Boscalid compounding are to late blight of potato Toxicity Determination
After determining the effective inhibition concentration scope of each medicament through prerun, each medicament is set 5 agent by active constituent content respectively
Gradient processing is measured, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, experiment is using mycelia life
Long performance rate method, by cultured pathogen, aseptically with a diameter of 5mm card punch, bacteria cake is cut from colony edge, is used
Bacteria cake is connected on the culture medium flat plate of different agents concentration by transfer needle, is put 28 DEG C, is cultivated in saturated humidity incubator；Treat control group
When bacterium colony is covered with, colony diameter is measured with crossing method, each chemicals treatment net growth, mycelial growth inhibition rate is calculated.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value.Simultaneously according to the abundant methods of Sun Yun
Co-toxicity coefficient method (CTC) evaluate the synergistic effect of pharmacy mix, i.e. CTC≤80, composition shows as antagonism, when 80
<CTC<120, composition shows as summation action, and when CTC >=120, composition shows as synergistic effect.Result of the test such as table 1.
The toxicity test analysis of results table of the Chinese yam polysaccharide of table 1, Boscalid and its compounding to the late blight of potato
| Reagent agent | Proportioning | Regression equation (y=a+bx) | Coefficient correlation | EC50(μg/mL) | Co-toxicity coefficient |
| Chinese yam polysaccharide (A) | / | Y=1.0993+1.5445x | 0.9934 | 335.42 | / |
| Boscalid (B) | / | Y=4.2739+1.7247x | 0.9986 | 2.64 | / |
| Fluopicolide (C) | / | Y=4.7653+1.7400x | 0.9951 | 1.36 | / |
| A：B | 30:1 | Y=2.3927+1.5097x | 0.9940 | 53.34 | 124.1 |
| A：B | 25:1 | Y=2.5413+1.4898x | 0.9860 | 44.71 | 128.3 |
| A：B | 20:1 | Y=2.2623+1.8004x | 0.9933 | 33.16 | 144.5 |
| A：B | 10:1 | Y=3.1690+1.5134x | 0.9926 | 16.21 | 166.1 |
| A：B | 1:1 | Y=4.3368+1.4544x | 0.9921 | 2.86 | 183.2 |
| A：B | 1:5 | Y=4.5531+1.7188x | 0.9945 | 1.82 | 173.8 |
| A：B | 1:10 | Y=4.4982+1.7469x | 0.9953 | 1.94 | 149.6 |
| A：B | 1:15 | Y=4.3837+1.6925x | 0.9905 | 2.31 | 121.8 |
| A：B | 40:1 | Y=2.1335+1.4097x | 0.9887 | 107.99 | 76.2 |
| A：B | 1:20 | Y=4.2482+1.7832x | 0.9842 | 2.64 | 105.0 |
| A：C | 25:1 | Y=2.6596+1.6152x | 0.9915 | 28.12 | 114.2 |
As shown in Table 1, the weight ratio of Chinese yam polysaccharide and Boscalid is 30:1-1:Mixture is to potato late blight in the range of 15
Disease shows obvious synergistic effect.Wherein, Chinese yam polysaccharide and Boscalid in weight ratio 20:1-1:Synergy in the range of 10
Effect is especially prominent.And the weight ratio of Chinese yam polysaccharide and fluopicolide is 25:When 1, composition shows as summation action.
Implementation of an application example two：The implementation of Formulation Example
Embodiment 1：50% Chinese yam polysaccharide Boscalid water dispersible granules
Chinese yam polysaccharide 25%, Boscalid 25%, polycarboxylate 4.5%, neopelex 3.5%, starch
4%th, sodium carbonate 2%, attapulgite complement to 100%, and each component is well mixed, after being crushed through airslide disintegrating mill, stirring
30min, is mediated, and is then added and is granulated in fluid bed, is dried after granulation is good, is sieved i.e. obtained 50% Chinese yam polysaccharide
Boscalid water dispersible granules.
Embodiment 2：63% Chinese yam polysaccharide Boscalid water dispersible granules
Chinese yam polysaccharide 60%, Boscalid 3%, aliphatic alcohol polyoxyvinethene phosphate 5%, lauryl sodium sulfate
4.5%th, polyvinyl alcohol 3%, potassium chloride 4.5%, white carbon 10%, kaolin complement to 100%, and each component is well mixed,
After being crushed through airslide disintegrating mill, 30min is stirred, is mediated, then adds and is granulated in fluid bed, be dried after granulation is good,
63% Chinese yam polysaccharide Boscalid water dispersible granules are made in screening.
Embodiment 3：48% Chinese yam polysaccharide Boscalid suspending agent
Chinese yam polysaccharide 8%, Boscalid 40%, APES 4.5%, lauryl sodium sulfate 4%, I
Primary glue 2%, glycerine 2.5%, organosilicon 0.05%, deionized water complement to 100%, first by Chinese yam polysaccharide, Boscalid and
Portions of de-ionized water is ground in sand mill, after the completion of grinding, and material is pumped into high-speed shearing machine, remaining component is added and enters
Row high speed shear, obtained 48% Chinese yam polysaccharide Boscalid suspending agent after the completion of shearing.
Embodiment 4：44% Chinese yam polysaccharide Boscalid suspending agent
Chinese yam polysaccharide 4%, Boscalid 40%, aliphatic alcohol polyoxyvinethene phosphate 3.5%, pull open powder 2%, xanthans
2%th, ethylene glycol 3%, organosilicon 0.05%, deionized water complement to 100%, first go to Chinese yam polysaccharide, Boscalid and part
Ionized water is ground in sand mill, after the completion of grinding, and material is pumped into high-speed shearing machine, is added remaining component and is carried out at a high speed
Shearing, obtained 44% Chinese yam polysaccharide Boscalid suspending agent after the completion of shearing.
Implementation of an application example three：The implementation of Formulation Example is applied to prevent and treat the late blight of potato test of pesticide effectiveness
This experimental arrangement is in Nanning suburbs, test method reference《Pesticide field efficacy medicine test criterion (one) bactericide
Prevent and treat late blight of potato GB/T17980.34-2000》, it is consistent or almost consistent sick seedling is used as experiment from disease index
Object.Experiment sets 7 processing altogether, often handles 4 repetitions, per plot area 25m2, totally 28 cells, random district's groups arrangement.
Investigation and statistical method：Using conventional spraying, blade positive and negative are uniformly squirted.In disease early period of origination for the first time
Dispenser, second of dispenser after 14 days, common dispenser is twice.Disease index investigation is carried out within 14 days after second of dispenser, per cell pair
5 points of samplings of linea angulata, every is investigated 2-3 plants, and the whole blades of investigation record total number of sheets, the sick number of sheets at different levels and sick series, calculate the state of an illness
Index.
Grade scale is：
0 grade, disease-free spot；
1 grade, lesion area accounts for less than the 5% of whole leaf area；
3 grades, lesion area accounts for the 6%-10% of whole leaf area；
5 grades, lesion area accounts for the 11%-20% of whole leaf area；
7 grades, lesion area accounts for the 21%-50% of whole leaf area；
9 grades, lesion area accounts for more than the 50% of whole leaf area.
Disease index and prevention effect computational methods：
Disease index=Σ (the sick numbers of sheets at different levels × relative value of series)/(investigation total number of sheets × 9) × 100
Prevention effect (%)=[(disease index after disease index-chemicals treatment district dispenser after blank district dispenser)/it is empty
Disease index after white check plot dispenser] × 100
The Chinese yam polysaccharide of table 2, Boscalid and its compounding prevent and treat the late blight of potato test of pesticide effectiveness
As can be seen from Table 2, Chinese yam polysaccharide and the bactericidal composition of Boscalid compounding can effectively prevent and treat potato late blight
The development of disease, prevention effect is better than single dose, reduces Pesticide use cost.
Claims (4)
1. a kind of bactericidal composition for preventing and treating the late blight of potato, it is characterised in that active ingredient is by Chinese yam polysaccharide and pyridine acyl bacterium
Amine is constituted, and the weight ratio of Chinese yam polysaccharide and Boscalid is 30:1-1:15.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of Chinese yam polysaccharide and Boscalid is
20:1-1:10。
3. bactericidal composition according to claim 1 or 2, it is characterised in that described active ingredient accounts for bactericidal composition
The 1-70% of gross weight.
4. the bactericidal composition according to claim any one of 1-3, it is characterised in that described bactericidal composition is prepared into
Water dispersible granules or suspending agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711845A (en) * | 2017-09-29 | 2018-02-23 | 杨凌农科大无公害农药研究服务中心 | A kind of pipering and Boscalid synergistic complex bactericide and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828812A (en) * | 2014-02-27 | 2014-06-04 | 海南正业中农高科股份有限公司 | Composition containing oligosaccharide and miazines bactericidal ingredients\ |
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- 2017-04-17 CN CN201710247195.3A patent/CN107041369A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828812A (en) * | 2014-02-27 | 2014-06-04 | 海南正业中农高科股份有限公司 | Composition containing oligosaccharide and miazines bactericidal ingredients\ |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711845A (en) * | 2017-09-29 | 2018-02-23 | 杨凌农科大无公害农药研究服务中心 | A kind of pipering and Boscalid synergistic complex bactericide and its application |
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Application publication date: 20170815 |