CN107006528A - A kind of bactericidal composition containing Herba Asari extract and zoxamide - Google Patents
A kind of bactericidal composition containing Herba Asari extract and zoxamide Download PDFInfo
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- CN107006528A CN107006528A CN201710339278.5A CN201710339278A CN107006528A CN 107006528 A CN107006528 A CN 107006528A CN 201710339278 A CN201710339278 A CN 201710339278A CN 107006528 A CN107006528 A CN 107006528A
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- Prior art keywords
- zoxamide
- herba asari
- asari extract
- bactericidal composition
- extract
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bactericidal composition containing Herba Asari extract and zoxamide, active ingredient is made up of Herba Asari extract and zoxamide, and the weight ratio of Herba Asari extract and zoxamide is 1:2‑15.The bactericidal composition that the present invention carries out mixture by Herba Asari extract and zoxamide is used to prevent and treat the late blight of potato, with synergistic function, can suppress infecting for disease, reaches disease resistance and the effect increased production.And the generation of disease drug resistance can be delayed, Pesticide use amount is reduced, low-residual is degradable in soil after the lasting period, environmentally friendly to crop safety.
Description
Technical field
The invention belongs to pesticides application technical field, and in particular to a kind of sterilization group containing Herba Asari extract and zoxamide
Compound.
Background technology
Potato (scientific name：Solanum tuberosum L.), belong to Solanaceae herbaceos perennial, stem tuber can be edible,
It is the fourth-largest important cereal crops in the whole world, is only second to wheat, paddy and corn.China is the most state of world's potato total yield
Family.The late blight of potato (Potato Late Blight) is caused by phytophthora infestans, causes potato haulm dead and stem tuber is rotten
A kind of rotten crushing oomycetes disease.All over the world, there is generation in potato producing region to the late blight of potato, typically subtracts in popular year
Production 30%.It is more serious in the regional potato place of production harm of Southwestern China, and northeast, North China and northwest rainy moist time be
Evil is heavier.
Asarum, alias：Careless asarum of asarum sieboldii, basin etc., Latin literary fame：Asarum sieboldii Miq.Belong to Aristolochiaceae,
Herbaceos perennial, is conventional Chinese medicine,《Sheng Nong's herbal classic》It is classified as top grade.This kind of all herbal medicine.HERBA ASARI from Liaoning of China root is thin, color
Sallow, leaf color are green, pungent peppery numb tongue.It is warm-natured, it is slightly poisonous.The thoughts of returning home, lung, kidney channel.There is a wind-dispelling, cold dispelling, warm lung, water-filling are had one's ideas straightened out
Effect.In addition, asarum also has anti inflammatory immunity, local anaesthesia, metabolism, antibacterial action is improved, also available for tumor patient
Neuroleptic Leukocytopenia caused by chemotherapy and radiation.Show Herba Asari extract to gram positive bacteria, hay bacillus and wound through experimental study
Cold bacillus has certain In-vitro Inhibitory Effect, has suppression to a variety of fungies such as Aspergillus flavus, black-koji mould, Candida albicans etc.
Effect.
Zoxamide (zoxamide) is a kind of efficient protective fungicide, is mainly used in preventing and treating oomycetes diseases such as horse
Bell potato and tomato late blight, cucumber downy mildew and downy mildew of garpe etc..Current zoxamide is widely used in preventing and treating cucumber downy mildew
Disease.
Whether zoxamide or other bactericide are used alone for a long time, all easily disease is developed immunity to drugs, lead
The problem of dose of applying is increased, preventive effect is reduced.It is that reduction resistance risk and delaying drug resistance are produced, by the effective of the different mechanisms of action
Composition is compounded, using being one of effective method.
At present the research of the late blight of potato is applied to using Herba Asari extract, zoxamide as the composition of active ingredient still
There is not relevant report.
The content of the invention
The technical problem to be solved in the present invention be to provide a kind of synergy significantly, reduce Pesticide use amount, safety and environmental protection and contain
The bactericidal composition of Herba Asari extract and zoxamide, for preventing and treating the late blight of potato.
The present invention is to be achieved through the following technical solutions：A kind of bactericidal composition containing Herba Asari extract and zoxamide,
Active ingredient is made up of Herba Asari extract and zoxamide, and the weight ratio of Herba Asari extract and zoxamide is 1:2-15.It is preferred that
The weight ratio of ground, Herba Asari extract and zoxamide is 1:10.
Described bactericidal composition active ingredient accounts for the 1-80% of bactericidal composition gross weight.
The Herba Asari extract is prepared from by the following method：Asarum herb is cleaned, dried, is crushed with pulverizer, mistake
40-60 mesh sieves, obtain crushed material；7-10 times is added in the crushed material of gained and measures 95% ethanol, is stored at room temperature mistake after extracting 3-5 days
Filter, obtains leaching liquor；Filter residue adds 7-10 times and measures 95% alcohol steep again, extracts again as stated above 1 time；Merging is extracted twice
Liquid, is then concentrated under reduced pressure into the medicinal extract that relative density is 1.15-1.20 with Rotary Evaporators, and powder is obtained after freeze-drying, that is, is made
Obtain Herba Asari extract.
Described bactericidal composition adds the agent that corresponding adjuvant is made according to method known in those skilled in the art
Type is wettable powder, water dispersible granules or suspending agent.
The adjuvant that bactericidal composition of the present invention is used includes dispersant, wetting agent, binding agent, disintegrant, antifreezing agent, disappeared
Infusion, thickener, filler, solvent etc. or it is other be beneficial to active ingredient in the formulation stablize and drug effect play known substance,
All it is the various composition commonly used in field of pesticide preparation or allow to use.
Beneficial effect of the present invention：The bactericidal composition that the present invention carries out mixture by Herba Asari extract and zoxamide is used for
The late blight of potato is prevented and treated, with synergistic function, infecting for disease can be suppressed, disease resistance and the effect increased production is reached.
And delay the generation of disease drug resistance, Pesticide use amount is reduced, low-residual is degradable in soil after the lasting period, to crop
Safety, it is environmentally friendly.
Embodiment
Technical scheme and technique effect are described further below in conjunction with specific embodiment, institute of the present invention
The percentage stated is weight percentage.
Herba Asari extract is prepared from by the following method：Asarum herb is cleaned, dried, is crushed with pulverizer, 50 mesh are crossed
Sieve, obtains crushed material；10 times of 95% ethanol of amount are added in the crushed material of gained, filters, must extract after being stored at room temperature extraction 5 days
Liquid；Filter residue adds 8 times of 95% alcohol steeps of amount again, extracts again as stated above 1 time；Merge leaching liquor twice, then with rotation
Turn to be freeze-dried to obtain powder after evaporation under reduced pressure is concentrated into the medicinal extract that relative density is 1.15, that is, Herba Asari extract is made.
First, Toxicity Determination is tested
After determining the effective inhibition concentration scope of each medicament through prerun, each medicament is set 5 agent by active constituent content respectively
Gradient processing is measured, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, experiment is using mycelia life
Long performance rate method, by cultured pathogen, aseptically with a diameter of 5mm card punch, bacteria cake is cut from colony edge, is used
Bacteria cake is connected on the culture medium flat plate of different agents concentration by transfer needle, is put 28 DEG C, is cultivated in saturated humidity incubator；Treat control group
When bacterium colony is covered with, colony diameter is measured with crossing method, each chemicals treatment net growth, mycelial growth inhibition rate is calculated.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value.Simultaneously according to the abundant methods of Sun Yun
Co-toxicity coefficient method (CTC) evaluate the synergistic effect of pharmacy mix, i.e. CTC≤80, composition shows as antagonism, when 80
<CTC<120, composition shows as summation action, and when CTC >=120, composition shows as synergistic effect.Result of the test such as table 1.
The toxicity test analysis of results table of the Herba Asari extract of table 1, zoxamide and its compounding to the late blight of potato
As shown in Table 1, the weight ratio of Herba Asari extract and zoxamide is 1:Mixture is to potato late blight in the range of 2-15
Disease shows obvious synergistic effect.Wherein, Herba Asari extract is 1 in weight ratio with zoxamide:Synergistic effect is outstanding when 10
For protrusion；And the weight ratio of Herba Asari extract and zoxamide is 1:When 18, composition shows as summation action；When asarum is extracted
The weight ratio of thing and zoxamide is 1:20、3:When 1, composition shows as antagonism.
2nd, product embodiments are prepared
Test group 1：11% Herba Asari extract zoxamide wettable powder (1:10)
Herba Asari extract (active ingredient) 1%, zoxamide (active ingredient) 10%, sodium carboxymethylcellulose (dispersant)
4%th, lauryl sodium sulfate (wetting agent) 3%, white carbon (filler) 10%, attapulgite (filler) complement to 100%；Will be each
Component is well mixed, and 11% Herba Asari extract zoxamide wettable powder is made after being pulverized and mixed in size-reduced machine.
Test group 2：14% Herba Asari extract zoxamide water dispersible granule (1:13)
Herba Asari extract (active ingredient) 1%, zoxamide (active ingredient) 13%, polycarboxylate (dispersant) 4.5%,
Calcium dodecyl benzene sulfonate (wetting agent) 3.5%, starch (binding agent) 2%, ammonium carbonate (disintegrant) 2%, kaolin (filler)
Complement to 100%；Each component is well mixed, after being crushed through airslide disintegrating mill, 30min is stirred, mediates, then add fluid bed
It is middle to be granulated, it is dried after granulation is good, sieves i.e. obtained 14% Herba Asari extract zoxamide water dispersible granule.Experiment
Group 3：6% Herba Asari extract zoxamide suspending agent (1:2)
Herba Asari extract (active ingredient) 2%, zoxamide (active ingredient) 4%, aliphatic alcohol polyoxyvinethene phosphate
(dispersant) 4.5%, calcium dodecyl benzene sulfonate (wetting agent) 3.5%, xanthans (thickener) 2%, ethylene glycol (antifreezing agent)
2%th, organic silicone oil (defoamer) 0.1%, deionized water (solvent) complement to 100%；First other auxiliary agents are mixed, in cutter
Middle shear-mixed is uniform, then adds Herba Asari extract and zoxamide, 3h is ground in ball crusher, you can 6% asarum is made
Extract zoxamide suspending agent.
Contrast groups 1：10% Herba Asari extract wettable powder
Herba Asari extract (active ingredient) 10%, sodium carboxymethylcellulose (dispersant) 4%, lauryl sodium sulfate are (wet
Profit agent) 3%, white carbon (filler) 10%, attapulgite (filler) complement to 100%；Each component is well mixed, size-reduced machine
10% Herba Asari extract wettable powder is made after being pulverized and mixed.
Contrast groups 2：10% zoxamide wettable powder
Zoxamide (active ingredient) 10%, sodium carboxymethylcellulose (dispersant) 4%, lauryl sodium sulfate (moistening
Agent) 3%, white carbon (filler) 10%, attapulgite (filler) complement to 100%；Each component is well mixed, size-reduced machine powder
10% zoxamide wettable powder is made after broken mixing.
Separately set blank control group.
3rd, embodiment is applied to prevent and treat the field medicine effect test of the late blight of potato
Test method：Reference《The part of GB/T17980.34-2000 pesticide field efficacy medicine tests criterion (one) the 34th：Sterilization
Agent prevents and treats the late blight of potato》Test method.First time spray pesticide at the initial stage of a disease, second of spray pesticide after 10 days,
Common dispenser is twice.Experiment sets 6 processing altogether, often handles 4 repetitions, and each plot area is 40m2, cell processing is using random
District's groups are arranged.Test site：Guangxi Guigang.
Investigation method：Before dispenser, 7 days after second of dispenser, 5 sampling surveys are carried out in each cell respectively within 14 days,
Every takes continuous 3 plants, looks into whole blades, and the classification record incidence in units of blade, calculates disease index and preventing and treating is imitated
Really.
Disease scale standard is：
0 grade, disease-free spot；
1 grade, lesion area accounts for less than the 5% of whole leaf area；
3 grades, lesion area accounts for the 6%-10% of whole leaf area；
5 grades, lesion area accounts for the 11%-20% of whole leaf area；
7 grades, lesion area accounts for the 21%-50% of whole leaf area；
9 grades, lesion area accounts for more than the 50% of whole leaf area.
Disease index and prevention effect computational methods：
Formula (1)：Disease index calculation formula：
In formula (1)：X- disease indexs；Ni- sick the number of sheets at different levels；I- is with respect to value of series；N- investigates total number of sheets.
Formula (2)：Prevention effect calculation formula：
In formula (2)：P- prevention effects, unit is percentage (%)；CK0Disease index before the dispenser of-blank district；CK1- blank
Disease index after the dispenser of check plot；PT0Disease index before the dispenser of-chemicals treatment district；PT1The state of an illness refers to after the dispenser of-chemicals treatment district
Number.
And yield investigation is carried out when potato harvest, calculate per mu yield and rate of growth.As a result it is as shown in table 2 below.
The Herba Asari extract of table 2, zoxamide and its compounding prevent and treat late blight of potato field medicine effect test
As can be seen from Table 2, the bactericidal composition that the present invention is compounded using Herba Asari extract and zoxamide as active ingredient
The late blight of potato can be effectively prevented and treated, prevention effect is better than single dose, reduces Pesticide use cost.And delayed the germ resistance to the action of a drug
Generation, low-residual is degradable in soil after the lasting period, environmentally friendly to crop safety, meets agricultural production
It is required that.
Claims (5)
1. a kind of bactericidal composition containing Herba Asari extract and zoxamide, it is characterised in that active ingredient is by Herba Asari extract
With zoxamide composition, the weight ratio of Herba Asari extract and zoxamide is 1:2-15.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of Herba Asari extract and zoxamide is
1:10。
3. bactericidal composition according to claim 1 or 2, it is characterised in that described active ingredient accounts for bactericidal composition
The 1-80% of gross weight.
4. the bactericidal composition according to claim any one of 1-3, it is characterised in that described bactericidal composition is prepared into
Wettable powder, water dispersible granules or suspending agent.
5. the bactericidal composition according to claim any one of 1-3, it is characterised in that described bactericidal composition is used to prevent
Control the late blight of potato.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710339278.5A CN107006528A (en) | 2017-05-15 | 2017-05-15 | A kind of bactericidal composition containing Herba Asari extract and zoxamide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710339278.5A CN107006528A (en) | 2017-05-15 | 2017-05-15 | A kind of bactericidal composition containing Herba Asari extract and zoxamide |
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| CN107006528A true CN107006528A (en) | 2017-08-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980803A (en) * | 2017-11-28 | 2018-05-04 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition for preventing the late blight of potato |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653118A (en) * | 2009-07-31 | 2010-02-24 | 深圳诺普信农化股份有限公司 | Compound synergistic bactericidal composition |
| CN106561725A (en) * | 2016-10-20 | 2017-04-19 | 中国农业科学院棉花研究所 | Asarum-source botanical bactericide and preparation method and application thereof |
-
2017
- 2017-05-15 CN CN201710339278.5A patent/CN107006528A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653118A (en) * | 2009-07-31 | 2010-02-24 | 深圳诺普信农化股份有限公司 | Compound synergistic bactericidal composition |
| CN106561725A (en) * | 2016-10-20 | 2017-04-19 | 中国农业科学院棉花研究所 | Asarum-source botanical bactericide and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李蕊倩: "细辛提取物对镰刀菌抑菌作用的初步分析", 《山西农业大学学报(自然科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980803A (en) * | 2017-11-28 | 2018-05-04 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition for preventing the late blight of potato |
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