CN107019008B - Bactericidal composition for preventing and treating rice black-streaked dwarf disease - Google Patents

Bactericidal composition for preventing and treating rice black-streaked dwarf disease Download PDF

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CN107019008B
CN107019008B CN201710399190.2A CN201710399190A CN107019008B CN 107019008 B CN107019008 B CN 107019008B CN 201710399190 A CN201710399190 A CN 201710399190A CN 107019008 B CN107019008 B CN 107019008B
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dufulin
bactericidal composition
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turmeric extract
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李桂香
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Guangxi Nanning City Binyang County Jufeng Rice Co ltd
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Guangxi Nanning Tianyuan Jufeng Agricultural Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing heterocyclic radicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal

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Abstract

The invention relates to a bactericidal composition for preventing and treating rice black-streaked dwarf virus, which comprises the effective components of turmeric extract and Dufulin, wherein the weight ratio of the turmeric extract to the Dufulin is 1: 5-25. The turmeric extract and the Dufulin are compounded, so that the composition is used for preventing and treating the rice black-streaked dwarf virus, and has obvious synergistic effect, thereby reducing the using amount of chemical pesticides. The invention has the characteristics of delaying the generation of drug resistance of pathogenic bacteria, being safe and friendly to crops and environment and the like, and conforms to the sustainable development direction of agriculture.

Description

Bactericidal composition for preventing and treating rice black-streaked dwarf disease
Technical Field
The invention relates to the technical field of pesticide application, and particularly relates to a bactericidal composition for preventing and treating rice black-streaked dwarf disease.
Background
The chemical name of the Dufulin is N- [2- (4-methylbenzothiazolyl) ] -2-amino-2-fluorophenyl-O, O-diethyl phosphonate, the molecular formula is C19H22FN2O3PS, the Dufulin is supported by the national fifteen' project of attack, national 973 project, national science foundation and Guizhou industrial attack project and the like at the earliest by a green pesticide and agricultural bioengineering major laboratory of the Ministry of university of Guizhou and the Fine chemical research and development center of Guizhou university, a biogenic antiviral medicament, Dufulin, is finally researched and developed by taking α phosphoramidate as a lead, and then the Guizhou university transfers the related technical result to Guangxi rural Younguan company on 12.21 days in 2007. the Dufulin related project is identified by the national technical result, the Dufulin antiviral activity and action mechanism research result reaches the leading level, and the 30% Dufulin related preparation product is wettable in the Mingroi nationality of the Yonghuan Yihao national Fei nationality and Biochemical engineering product, namely, the Ningqing Yihao national Fei nationality product, namely, the Ningqing national Fei nationality and Bio:
(1) active defense: the Dufulin activates salicylic acid signal molecules, further activates plant defense factors such as downstream PAL, POD, SOD and the like, improves the disease resistance of the overall system of crops, and finally makes the virus incapable of proliferating.
(2) Systemic therapy: the Dufulin has stronger systemic action, can be quickly transmitted to each part of a plant through the absorption of crop leaves, destroys the virus shell, enables the virus to be fixed and cannot be continuously propagated, and effectively prevents the further spread of diseases.
(3) Promoting growth: the Dufulin can promote root growth, recover leaf function and reduce yield loss by regulating endogenous growth factors of plants.
(4) No cross resistance: dufulin is a new compound artificially synthesized for the first time all over the world, has unique action mechanism, belongs to a national patent product, and has no resistance in system protection.
(5) Safety and environmental protection: the Dufulin is a compound biomimetically synthesized by taking an aminophosphate compound in the rumen of the sheep as a lead, is highly safe to crops and is highly safe to non-target organisms such as bees, silkworms, fishes, birds and the like.
Turmeric, also known as: turmeric root-tuber, baodingxiang, Chinese character of 'mi', turmeric root-tuber, sliced yellow ginger, etc., which are derived from dried rhizome of curcuma longa L. Turmeric is produced in Taiwan, Fujian, Guangdong, Guangxi, Yunnan, Tibet, etc., and is widely cultivated in east Asia and southeast Asia. It has effects of activating qi-flowing, removing blood stasis, dredging channels and relieving pain, and can be used for treating chest and abdominal distending pain, shoulder and arm pain, heart pain, puerperal blood pain, sore and tinea, menoxenia, amenorrhea, and traumatic injury. But also can extract yellow edible dye, and the curcumin contained in the yellow edible dye can be used as an analytical chemical reagent. The curcumin compounds are natural effective components of Curcuma rhizome, and have effects of reducing blood lipid, resisting tumor, resisting inflammation, resisting pathogenic microorganism, etc.
Rice black-streaked dwarf disease (rice black-streaked dwarf disease) is a rice viral disease which is widely spread in rice regions in south China and takes place in rice producing areas all over the country, and seriously harms the quality and yield of rice, and is mainly carried out by using plant hoppers as main virus-transmitting mediators. The disease is discovered in the first 60 th of 20 th century in China. Since the 90 s in the 20 th century, the area of the disease in rice areas in Vietnam and southern China is rapidly enlarged, the damage degree is obviously increased, and particularly, in 2010, the disease causes no harvest of multiple grains in pieces of fields, thereby causing great threat to rice production in China.
The effective components with different action mechanisms are combined, the formula with obvious synergistic effect can greatly improve the control effect, reduce the dosage, reduce the cost and protect the environment, and simultaneously can avoid the generation of resistance of plant pathogenic bacteria or delay the generation speed of the resistance, thereby being an effective and quick way for solving the problems of single-use resistance and cost of the current pesticide. At present, no relevant report exists on the research of applying the composition taking turmeric extract and Dufulin as effective components to the prevention and treatment of the black-streaked dwarf virus.
Disclosure of Invention
The invention aims to solve the technical problem of providing a bactericidal composition which has good control effect, can reduce the use amount of chemical pesticides, is safe and friendly to crops, environment and the like and is used for controlling the rice black-streaked dwarf virus.
The technical scheme adopted by the invention for solving the technical problems is as follows: a bactericidal composition for preventing and treating rice black streaked dwarf disease comprises effective components of turmeric extract and Dufulin, wherein the weight ratio of the turmeric extract to the Dufulin is 1: 5-25. Preferably, the weight ratio of turmeric extract to Dufulin is 1: 10.
The effective component of the bactericidal composition accounts for 1-70% of the total weight of the bactericidal composition, and the balance is auxiliary components acceptable in pesticide.
The bactericidal composition is prepared into wettable powder or water dispersible granules by adding corresponding auxiliary ingredients according to a method known by a person skilled in the art.
The auxiliary ingredients used in the bactericidal composition of the present invention include dispersing agents, wetting agents, disintegrating agents, binders, stabilizers, fillers or other known substances which are beneficial to the stability of the active ingredients in the preparation and the exertion of the drug effect, and are various ingredients which are commonly used or allowed to be used in the field of pesticide preparations.
The preparation method of the turmeric extract comprises the following steps:
1) cleaning rhizome of Curcuma rhizome, drying in the shade, pulverizing with a pulverizer, and sieving with 40-60 mesh sieve;
2) putting the crushed turmeric into a reaction kettle, adding 95% ethanol in an amount which is 6-10 times that of the turmeric, soaking at room temperature for 12-48h, then leaching for 25-40min under ultrasonic waves, collecting leaching liquor, extracting for 1-2 times according to the method, and combining all the leaching liquor;
3) and filtering the obtained leaching liquor by using a Buchner funnel, concentrating the filtrate by using a rotary evaporator under reduced pressure to obtain an extract with the relative density of 1.10-1.20, and freeze-drying the extract to obtain powder so as to obtain the turmeric extract.
The invention has the beneficial effects that: the turmeric extract and the Dufulin are compounded, so that the composition is used for preventing and treating the rice black-streaked dwarf virus, and has obvious synergistic effect, thereby reducing the using amount of chemical pesticides. The invention has the characteristics of delaying the generation of drug resistance of pathogenic bacteria, being safe and friendly to crops and environment and the like, and conforms to the sustainable development direction of agriculture.
Detailed Description
The technical scheme and the technical effect of the invention are further explained by combining specific examples, and the percentages are weight percentages.
Preparation of turmeric extract
The preparation method of the turmeric extract comprises the following steps:
1) cleaning rhizome of Curcuma rhizome, drying in the shade, pulverizing with a pulverizer, and sieving with 40 mesh sieve;
2) putting the crushed turmeric into a reaction kettle, adding 10 times of 95% ethanol, soaking at room temperature for 24h, then leaching for 40min at 40 ℃ under 60Hz frequency ultrasonic wave, collecting leaching liquor, extracting for 2 times according to the method, and combining all the leaching liquor;
3) filtering the obtained leaching solution with Buchner funnel, concentrating the filtrate under reduced pressure with rotary evaporator to obtain extract with relative density of 1.15, and freeze drying the extract to obtain Curcuma rhizome extract.
Second, indoor toxicity determination
The test method comprises the following steps: the plant is used for feeding the planthopper, so that the planthopper is infected, and the detected toxicity carrying rate is about 35%. Potted healthy rice plants for inoculation of insects. When the seedlings grow to 1 leaf and 1 heart to 2 leaf and 1 heart, inoculation identification is carried out. Each pot is covered with a transparent cover, the top end of the pot is sealed by gauze, the insect quantity required is calculated according to 5 Laodelphax striatellus per seedling, and 2-3-year Laodelphax striatellus (with the toxicity rate about 35%) is inoculated. Expelling parasites 2 times a day to ensure that the rice seedlings to be detected are evenly poisoned. After 48h, the prepared medicaments with different concentrations are uniformly sprayed on the tested seedlings, the treatment is repeated for 4 times, and the seedlings treated grow and attack diseases under the condition of natural illumination. The test was run with treatment without agent as a blank. When the blank control is about 50% of the disease, the disease is graded and investigated, and the inhibition rate is calculated.
The inhibition rate was converted into a probability value (y), the drug solution concentration (μ g/mL) was converted into a logarithm value (x), and a regression equation of virulence (y ═ a + bx) was obtained by the least square method, and the EC of each drug was calculated from the equation50The value is obtained. Meanwhile, the synergistic effect of the mixed medicaments is evaluated according to the co-toxicity coefficient (CTC) method of the Sun Yunpei method, namely the CTC is less than or equal to 80, the composition shows antagonistic effect, and when the 80 is equal to 80<CTC<120, the composition shows additive effect, and when CTC is more than or equal to 120, the composition shows synergistic effect. The test results are shown in Table 1.
TABLE 1 toxicity test results analysis table of Curcuma rhizome extract, Dufulin and its combination for rice black streaked dwarf virus
Figure BDA0001309301120000031
Figure BDA0001309301120000041
As can be seen from Table 1, the weight ratio of the turmeric extract to Dufulin is in the range of 1:5-25, and the turmeric extract and Dufulin have obvious synergistic effect on the rice black streaked dwarf. Wherein, when the weight ratio of the turmeric extract to the Dufulin is 1:10, the synergistic effect is particularly outstanding, and the co-toxicity coefficient is 214.0; when the weight ratio of the curcuma extract to the Dufulin is 1:27 and 1:2, the composition shows additive effect; the composition exhibits antagonism when the weight ratio of turmeric extract to Dufulin is 3:1, 1: 30.
Third, examples of the formulation
Example 1: 22% turmeric extract-Dufulin wettable powder (1:10)
Weighing 2% of turmeric extract (effective component), 20% of Dufulin (effective component), 5% of alkylphenol polyoxyethylene (dispersing agent), 4.5% of Chinese honeylocust fruit powder (wetting agent), 10% of attapulgite (filler) and kaolin (filler) to make up to 100%; the components are uniformly mixed in a mixing cylinder, and are uniformly mixed after being crushed by a jet mill, so as to prepare the turmeric extract-Dufulin wettable powder with the concentration of 22 percent.
Example 2: 10.5% turmeric extract-Dufulin wettable powder (1:20)
Weighing Curcuma rhizome extract (effective component) 0.5%, Dufulin (effective component) 10%, lignosulfonate (dispersant) 3.5%, sodium dodecyl sulfate (wetting agent) 3%, and diatomaceous earth (filler) to make up to 100%; the components are uniformly mixed in a mixing cylinder, and are uniformly mixed after being crushed by a jet mill, so as to prepare the turmeric extract-Dufulin wettable powder with the concentration of 10.5 percent.
Example 3: 32% turmeric extract-Dufulin wettable powder (1:15)
Weighing 2% of turmeric extract (effective component), 30% of Dufulin (effective component), 3% of lignosulfonate (dispersant), 3.5% of alkylbenzene sulfonate (dispersant), 5% of nekal (wetting agent) and soil powder (filler) to complement to 100%; the components are uniformly mixed in a mixing cylinder, and are uniformly mixed after being crushed by a jet mill, so as to prepare the 32 percent turmeric extract-Dufulin wettable powder.
Example 4: 18% turmeric extract-Dufulin water dispersible granule (1:5)
Weighing 3% of turmeric extract (effective component), 15% of Dufulin (effective component), 4% of nonylphenol polyoxyethylene ether (dispersant), 4% of calcium dodecylbenzene sulfonate (wetting agent), 2% of starch (binder), 2% of ammonium carbonate (disintegrant), 8% of white carbon black (filler) and kaolin (filler) to make up to 100%; pulverizing the above materials with air flow to obtain desired particle size, granulating, and drying to obtain 18% Curcuma rhizome extract-Dufulin water dispersible granule.
Example 5: 26% turmeric extract Dufulin Water dispersible granule (1:25)
Weighing 1% of turmeric extract (effective component), 25% of Dufulin (effective component), 5.5% of alkylphenol polyoxyethylene (dispersant), 5% of sodium dodecyl sulfate (wetting agent), 2.5% of sodium carboxymethylcellulose (binder), 2% of sodium carbonate (disintegrant) and diatomite (filler) to make up to 100%; pulverizing the above materials with air flow to obtain desired particle size, granulating, and drying to obtain 26% Curcuma rhizome extract-Dufulin water dispersible granule.
Example 6: 16% turmeric extract-Dufulin water dispersible granule (1:7)
Weighing 2% of turmeric extract (effective component), 14% of Dufulin (effective component), 4% of alkyl naphthalene sulfonate formaldehyde condensate (dispersant), 3.5% of calcium dodecyl benzene sulfonate (wetting agent), 2% of polyvinyl alcohol (binder), 2% of calcium chloride (disintegrant), 8% of white carbon black (filler) and kaolin (filler) to complement to 100%; pulverizing the above materials with air flow to obtain desired particle size, granulating, and drying to obtain 16% Curcuma rhizome extract-Dufulin water dispersible granule.
Comparative example 1: 22% turmeric extract wettable powder
Weighing 22% of turmeric extract (effective component), 5% of alkylphenol polyoxyethylene ether (dispersant), 4.5% of Chinese honeylocust fruit powder (wetting agent), 10% of attapulgite (filler) and kaolin (filler) to make up to 100%; the components are uniformly mixed in a mixing cylinder, and are uniformly mixed after being crushed by a jet mill, so that 22 percent turmeric extract wettable powder can be prepared.
Comparative example 2: 30% Dufulin wettable powder, registration certificate number: PD20160338, Guangxi rural Biochemical Co., Ltd, commercially available.
A blank control was also set.
Fourth, the embodiment of the formula is applied to the field pesticide effect test for preventing and treating the rice black-streaked dwarf disease
The test method comprises the following steps: the experiment is arranged in Guangxi Bingyang county, the first spraying pesticide application is carried out 10-15 days after the rice is transplanted, the second spraying pesticide application is carried out 7 days after the first pesticide application, and the pesticide application is carried out twice. The experiment was carried out with a total of 7 treatments, 4 replicates per treatment, each cell area of 40m2Random block permutation.
The investigation method comprises the following steps: the disease index and the prevention and treatment effect are calculated according to the relevant data survey grading standard by randomly sampling 5 points in each cell and surveying 70 clusters in each cell before drug application and 7 days and 14 days after the second drug application.
The calculation method of disease index and prevention and treatment effect comprises the following steps:
the disease index calculation formula is as follows:
Figure BDA0001309301120000061
in the formula:
x-disease index; n is a radical ofi-number of diseased leaves at each level; i-relative grade value; n-survey total leaf number.
The prevention and treatment effect formula is as follows:
Figure BDA0001309301120000062
in the formula:
p-control effect, unit is percentage (%); CK (CK)0-pre-drug disease index in placebo zone; CK (CK)1-disease index after drug administration in placebo zone; PT0-pre-dose disease index in the agent treatment area; PT1-disease index after drug treatment area application.
TABLE 2 field test of the effect of turmeric extract, Dufulin and its combination on the prevention and treatment of black streaked dwarf of rice
Figure BDA0001309301120000063
As can be seen from the table 2, the bactericidal composition compounded by the turmeric extract and the Dufulin can effectively prevent and treat the rice black streaked dwarf disease, and the prevention and treatment effects are better than those of a single agent. The two are compounded, the generation of drug resistance of germs can be delayed, no obvious adverse effect is caused to test crops, the safety is good, and the safety requirement of pesticide preparations is met.

Claims (4)

1. A bactericidal composition for preventing and treating rice black streaked dwarf disease is characterized in that the effective components consist of turmeric extract and Dufulin, and the weight ratio of the turmeric extract to the Dufulin is 1: 5-25;
the preparation method of the turmeric extract comprises the following steps:
1) cleaning rhizome of Curcuma rhizome, drying in the shade, pulverizing with a pulverizer, and sieving with 40 mesh sieve;
2) putting the crushed turmeric into a reaction kettle, adding 10 times of 95% ethanol, soaking at room temperature for 24h, then leaching for 40min at 40 ℃ under 60Hz frequency ultrasonic wave, collecting leaching liquor, extracting for 2 times according to the method, and combining all the leaching liquor;
3) filtering the obtained leaching solution with Buchner funnel, concentrating the filtrate under reduced pressure with rotary evaporator to obtain extract with relative density of 1.15, and freeze drying the extract to obtain Curcuma rhizome extract.
2. The bactericidal composition of claim 1, wherein the weight ratio of turmeric extract to Dufulin is 1: 10.
3. The bactericidal composition of claim 1 or 2, wherein the active ingredient comprises 1-70% of the total weight of the bactericidal composition, and the balance is an agriculturally acceptable auxiliary ingredient.
4. The bactericidal composition of claim 3, wherein the bactericidal composition is prepared as wettable powder or water dispersible granules.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1947522A (en) * 2005-10-14 2007-04-18 中国医学科学院药用植物研究所 Botany resource disinfectants for agricultural use an dits prepn. method
CN102349538A (en) * 2011-11-04 2012-02-15 广西田园生化股份有限公司 Anti-plant virus pesticide composition
CN102428956A (en) * 2011-11-04 2012-05-02 广西田园生化股份有限公司 Insecticidal antivirus composition based on Dufulin and insecticide
CN103814943A (en) * 2014-02-13 2014-05-28 山东省农业科学院植物保护研究所 Pesticide composite for preventing and controlling plant virus disease and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1947522A (en) * 2005-10-14 2007-04-18 中国医学科学院药用植物研究所 Botany resource disinfectants for agricultural use an dits prepn. method
CN102349538A (en) * 2011-11-04 2012-02-15 广西田园生化股份有限公司 Anti-plant virus pesticide composition
CN102428956A (en) * 2011-11-04 2012-05-02 广西田园生化股份有限公司 Insecticidal antivirus composition based on Dufulin and insecticide
CN103814943A (en) * 2014-02-13 2014-05-28 山东省农业科学院植物保护研究所 Pesticide composite for preventing and controlling plant virus disease and application thereof

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