The content of the invention
Present invention aim at the Magnolia wufengensis nursery stock spring embedding bud cutting skin grafting side that a kind of survival rate of offer is up to 80%
Method, high graft survival rate, but grafting condition is more strict.
To solve above technical problem, it is embedding that the technical scheme that the present invention is provided is to provide a kind of Magnolia wufengensis nursery stock spring
Bud cutting skin engrafting method, the previous year eugonic shoot is belonged to as stock with yulan, with Magnolia wufengensis current-year branch as scion,
2 months first arrival late March carry out grafting work, and all grafting works must be completed before stock rudiment, grafting temperature along the Changjiang river
It is adapted at 4-18 DEG C, northern grafting temperature is adapted at 8-18 DEG C;
Hide bud:During winter dormancy is entered, first Magnolia wufengensis current-year branch is cut as scion, load cloth bag or gunnysack
Imbedded below 1 meter or so of underground pit excavation or frozen soil layer, first layer overlay 30cm is thick husky again with earthen backfill covering, grafting to be needed
When take out;
Stock:Selection the previous year, eugonic shoot was stock, and the angular cut of inside cutting one of bast is close in stock,
Then from top from top to bottom vertical sliding cutting to forming a block length tangent plane at angular cut;
Cut embedding bud cutting skin:Knife face is cut into 180 ° of planes at the embedding bud cutting skin side 0.8-1cm of scion;
Grafting:The embedding bud cutting skin of scion after cutting is docked in the forming layer of both stock tangent planes long, reaches its identical rate
To very, so as to form grafting;
Sealer:The embedding bud cutting skin of scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to twine
Wrap up in it is tight can not intake, bud-leaf exposes;
Cut anvil:Sprouting after to next year grafting is long to 15cm, and stock on bud is all cut.
Further, the sunny or cloudy weather of the engrafting method selection is carried out.
Further, a length of 4-5cm of stock tangent plane long, a width of 0.5-1.5cm;The embedding a length of 3-5cm of bud cutting skin of scion.
Further, along the Changjiang river grafting temperature at 6~15 DEG C.
Further, northern grafting temperature is at 8~15 DEG C.
Further, during grafting, after scion insertion stock, the table of scion and stock joint is sprayed on exocellular polysaccharide
Face.
Compared with prior art, this graft technology has advantages below:
1st, graft survival rate is up to more than 80%, is conducive to the further raising of Magnolia wufengensis nursery stock spring grafting technology to send out
Exhibition, improves engrafting method;
2nd, plant resistance against diseases, low temperature tolerance ability can be strengthened, solving traditional Magnolia wufengensis grafting can only autumn scope
And ability, to amount reproduction Magnolia wufengensis, this rare variety plays an important roll.
Specific embodiment
Respective description is carried out below in conjunction with instantiation.This example is that principle of the invention and feature are described, only
For explaining that the technology of the present invention is not intended to limit the scope of the technology of the present invention.
Method one
A kind of Magnolia wufengensis nursery stock spring embedding bud cutting skin engrafting method, the previous year eugonic shoot is belonged to as anvil with yulan
Wood, with Magnolia wufengensis current-year branch as scion, first arrival late March carries out grafting within 2 months, and grafting temperature is 6~15 along the Changjiang river
DEG C, all grafting works must assure that and completed before stock rudiment, the embedding bud cutting skin for Magnolia wufengensis nursery stock spring grafting
Cut-grafting method solves the extremely low problem of Magnolia wufengensis nursery stock spring grafting survival rate, using this kind of engrafting method in Magnolia wufengensis
The effect plugged a gap is served in the grafting work in nursery stock spring,.
Magnolia wufengensis spring embedding bud cutting skin engrafting method in this example, comprises the following steps that:
1) bud is hidden:During winter dormancy is entered, first Magnolia wufengensis current-year branch (i.e. scion) is cut, load cloth bag or fiber crops
Bag is imbedded 1 meter or so of underground pit excavation (below frozen soil layer), first with being covered with earthen backfill again after the thick sand of layer overlay 30cm, is treated
Need to be taken out during grafting.
2) stock:Selection the previous year eugonic shoot first is stock, and the inside cutting one of bast is close in stock
Angular cut, then from top, from top to bottom vertical sliding cutting requires that its length is cut to a block length tangent plane, the step is formed at angular cut
The surfacing in face is smooth, and its forming layer is obvious.
3) embedding bud cutting skin is cut:Knife face is cut into 180 ° of planes at the embedding bud cutting skin side 0.8-1cm of scion.
4) grafting:The embedding bud cutting skin of scion after cutting is docked in the forming layer of both stock tangent planes long, makes its identical rate
Reach very, so as to form grafting.
5) sealer:The embedding bud cutting skin of scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to
Swathe it is tight can not intake, bud-leaf exposes.
6) cut anvil:Sprouting after to next year grafting is long to 15cm, and stock on bud is all cut.
By above method cut-grafting Magnolia wufengensis nursery stock spring embedding bud cutting skin, empirical tests:Graft survival rate be up to 80% with
On, be conducive to the further raising of Magnolia wufengensis nursery stock spring grafting technology to develop, while saving grafting cost, improving grafting side
Method, plays an important roll to amount reproduction Magnolia wufengensis this rare variety of nursery stock spring.
Method two
Magnolia wufengensis spring, embedding bud cutting skin engrafting method also included:During grafting after scion insertion stock, sprayed with exocellular polysaccharide
It is coated in the surface of scion and stock joint.
Exocellular polysaccharide, be some peculiar microorganisms be secreted into that cell membrane is outer during growth metabolism, easily separated with thalline,
The water-soluble polysaccharide in environment is secreted into, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide (exopolysaccharides, EPS):It is that some peculiar microorganisms are secreted during growth metabolism
Water-soluble polysaccharide that is outer to cell membrane, easily being separated with thalline, be secreted into environment, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide microbial polysaccharide compared with plant polyose has unique and excellent physical characteristic, such as in low concentration
Under, viscosity can be increased, without forming gel;Small shearforce, can quickly increase mobility, and reduce viscosity, as long as
Shearforce is eliminated, can extensive revival rapidly.The glucan produced by leuconostoc dextranicum bacteria can as column material, big point
Sub- modifying agent etc..
Experiment:
Above engrafting method one (experimental group 1), method two (experimental group 2) is (right with traditional engrafting method simple bud cutting method
1) and with wooden cortex grafting (control group 2) Experimental comparison is carried out according to group.Each experimental group randomly chooses three skills involved in the labour one
The grafting workman of cause carries out grafting to 100 plants of Magnolia wufengensis, so that by respective grafting situation to three kinds of survival rates of method
It is compared with the labor intensive time.It is as shown in the table:
|
Experimental group 1 |
Experimental group 2 |
Control group 1 |
Control group 2 |
Grafting quantity |
100 |
100 |
100 |
100 |
Survive quantity |
80 |
82 |
15 |
10 |
Survival rate |
80% |
82% |
15% |
10% |
The consuming time/h |
2.1 |
2.2 |
2 |
2 |
Test result indicate that:By this graft technology inventive method, one is that to belong to 1 year raw Magnolia wufengensis nursery stock new due to yulan
Branch, vitality, growing way are strong, easily form callus;Two is that scion dormancy is hidden into bud, the formation of the embedding bud cutting skin of cut-grafting and stock
Layer laminating closely, parenchyma cell is adjacent to, and joint is closely sealed, produces the ability of callus strong between stock and scion, is easily formed
The materials such as new conducting tissue, moisture and nutrition can be transported in place;Three is that Magnolia wufengensis nursery stock spring grafting mouthful faces south, just
In the position daylighting of grafting bud, the favourable embedding bud cutting skin of grafting and stock heal.80% survival is can reach using this kind of engrafting method
Rate, far above the survival rate of two other control group 10% and 15%.Therefore this grafting invention solves Magnolia wufengensis well
The survival rate of nursery stock spring grafting, this grafting and breeding to Magnolia wufengensis nursery stock spring has material impact.
Experimental group 2, using exocellular polysaccharide plant surface formed viscosity, without formed gel the characteristics of, can improve
Scion and stock connective stability, can also fill the space of special grafting membrane and plant surface, again will not be to plant growth shape
Into obstruction, so as to further increase plant survival rate.
The above is only the preferred embodiment of this graft technology invention, protection scope of the present invention should be with claim institute
The scope of restriction is defined.For those skilled in the art, without departing from the spirit and scope of the present invention
Can also make it is some improvement and it is perfect, these improve and improve should also be considered as protection scope of the present invention.