CN106856995A - Magnolia wufengensis nursery stock spring embedding bud cutting skin engrafting method - Google Patents

Magnolia wufengensis nursery stock spring embedding bud cutting skin engrafting method Download PDF

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Publication number
CN106856995A
CN106856995A CN201710048461.XA CN201710048461A CN106856995A CN 106856995 A CN106856995 A CN 106856995A CN 201710048461 A CN201710048461 A CN 201710048461A CN 106856995 A CN106856995 A CN 106856995A
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China
Prior art keywords
grafting
stock
cut
cutting skin
embedding
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CN201710048461.XA
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Chinese (zh)
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CN106856995B (en
Inventor
李承荣
严洪盛
陈邦清
万晓峰
李子玉
张新
吕德新
朱丽
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Yichang Forest Seedling Management Station
Hubei Sen Ecological Forestry Ltd By Share Ltd
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Yichang Forest Seedling Management Station
Hubei Sen Ecological Forestry Ltd By Share Ltd
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Priority to CN201710048461.XA priority Critical patent/CN106856995B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G3/00Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
    • A01G2003/005Removing buds

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention discloses a kind of embedding bud cutting skin engrafting method of Magnolia wufengensis nursery stock spring, nursery stock is belonged to as stock with yulan, with Magnolia wufengensis current-year branch as scion, grafting work is carried out in first arrival in 2 months mid-March, grafting temperature is adapted at 4 18 DEG C along the Changjiang river, northern grafting temperature is adapted at 8 18 DEG C, and grafting procedures include hiding bud, cut stock, cut bud, grafting, twine film, six steps of cut anvil.It is an advantage of the invention that, this kind of engrafting method can reach more than 80% survival rate, rear current growth height is survived up to more than 1.5 meters, embedding bud cutting skin cut-grafting method solves the extremely low problem of Magnolia wufengensis nursery stock spring grafting survival rate for Magnolia wufengensis nursery stock spring grafting.

Description

Magnolia wufengensis nursery stock spring embedding bud cutting skin engrafting method
Technical field
The invention belongs to seedling propagation field, it is related to the engrafting method of plant, and in particular to the spring of Magnolia wufengensis nursery stock Embedding bud cutting skin engrafting method.
Background technology
Magnolia wufengensis (Magnolia wufengensis), also known as five peak yulans, are Beijing Forestry University Ma Lvyi professors Et al. carry out the Magnoliacea plant novel species that finds when Plant Resource of Magnoliaceae is investigated at five peaks within 2004, only Chinese five peak is exclusive. Magnolia wufengensis belong to megaphanerophyte high, and tree-like grace, and with extremely abundant flower morphology variation property, tapel number is from 9 valves To 46 valves, floral color type has dark red, red with pale red different manifestations, and ornamental value is high, is splendid Evergreen garden plant Kind.
Current Magnolia wufengensis also are not fairly well-developed out in afforestation market, and mainly reproductive survival rate is too low, because This inventor changes a cut-grafting method using cleft grafting, increases substantially the survival rate of grafting nursery stock.
The content of the invention
Present invention aim at the Magnolia wufengensis nursery stock spring embedding bud cutting skin grafting side that a kind of survival rate of offer is up to 80% Method, high graft survival rate, but grafting condition is more strict.
To solve above technical problem, it is embedding that the technical scheme that the present invention is provided is to provide a kind of Magnolia wufengensis nursery stock spring Bud cutting skin engrafting method, the previous year eugonic shoot is belonged to as stock with yulan, with Magnolia wufengensis current-year branch as scion, 2 months first arrival late March carry out grafting work, and all grafting works must be completed before stock rudiment, grafting temperature along the Changjiang river It is adapted at 4-18 DEG C, northern grafting temperature is adapted at 8-18 DEG C;
Hide bud:During winter dormancy is entered, first Magnolia wufengensis current-year branch is cut as scion, load cloth bag or gunnysack Imbedded below 1 meter or so of underground pit excavation or frozen soil layer, first layer overlay 30cm is thick husky again with earthen backfill covering, grafting to be needed When take out;
Stock:Selection the previous year, eugonic shoot was stock, and the angular cut of inside cutting one of bast is close in stock, Then from top from top to bottom vertical sliding cutting to forming a block length tangent plane at angular cut;
Cut embedding bud cutting skin:Knife face is cut into 180 ° of planes at the embedding bud cutting skin side 0.8-1cm of scion;
Grafting:The embedding bud cutting skin of scion after cutting is docked in the forming layer of both stock tangent planes long, reaches its identical rate To very, so as to form grafting;
Sealer:The embedding bud cutting skin of scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to twine Wrap up in it is tight can not intake, bud-leaf exposes;
Cut anvil:Sprouting after to next year grafting is long to 15cm, and stock on bud is all cut.
Further, the sunny or cloudy weather of the engrafting method selection is carried out.
Further, a length of 4-5cm of stock tangent plane long, a width of 0.5-1.5cm;The embedding a length of 3-5cm of bud cutting skin of scion.
Further, along the Changjiang river grafting temperature at 6~15 DEG C.
Further, northern grafting temperature is at 8~15 DEG C.
Further, during grafting, after scion insertion stock, the table of scion and stock joint is sprayed on exocellular polysaccharide Face.
Compared with prior art, this graft technology has advantages below:
1st, graft survival rate is up to more than 80%, is conducive to the further raising of Magnolia wufengensis nursery stock spring grafting technology to send out Exhibition, improves engrafting method;
2nd, plant resistance against diseases, low temperature tolerance ability can be strengthened, solving traditional Magnolia wufengensis grafting can only autumn scope And ability, to amount reproduction Magnolia wufengensis, this rare variety plays an important roll.
Specific embodiment
Respective description is carried out below in conjunction with instantiation.This example is that principle of the invention and feature are described, only For explaining that the technology of the present invention is not intended to limit the scope of the technology of the present invention.
Method one
A kind of Magnolia wufengensis nursery stock spring embedding bud cutting skin engrafting method, the previous year eugonic shoot is belonged to as anvil with yulan Wood, with Magnolia wufengensis current-year branch as scion, first arrival late March carries out grafting within 2 months, and grafting temperature is 6~15 along the Changjiang river DEG C, all grafting works must assure that and completed before stock rudiment, the embedding bud cutting skin for Magnolia wufengensis nursery stock spring grafting Cut-grafting method solves the extremely low problem of Magnolia wufengensis nursery stock spring grafting survival rate, using this kind of engrafting method in Magnolia wufengensis The effect plugged a gap is served in the grafting work in nursery stock spring,.
Magnolia wufengensis spring embedding bud cutting skin engrafting method in this example, comprises the following steps that:
1) bud is hidden:During winter dormancy is entered, first Magnolia wufengensis current-year branch (i.e. scion) is cut, load cloth bag or fiber crops Bag is imbedded 1 meter or so of underground pit excavation (below frozen soil layer), first with being covered with earthen backfill again after the thick sand of layer overlay 30cm, is treated Need to be taken out during grafting.
2) stock:Selection the previous year eugonic shoot first is stock, and the inside cutting one of bast is close in stock Angular cut, then from top, from top to bottom vertical sliding cutting requires that its length is cut to a block length tangent plane, the step is formed at angular cut The surfacing in face is smooth, and its forming layer is obvious.
3) embedding bud cutting skin is cut:Knife face is cut into 180 ° of planes at the embedding bud cutting skin side 0.8-1cm of scion.
4) grafting:The embedding bud cutting skin of scion after cutting is docked in the forming layer of both stock tangent planes long, makes its identical rate Reach very, so as to form grafting.
5) sealer:The embedding bud cutting skin of scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to Swathe it is tight can not intake, bud-leaf exposes.
6) cut anvil:Sprouting after to next year grafting is long to 15cm, and stock on bud is all cut.
By above method cut-grafting Magnolia wufengensis nursery stock spring embedding bud cutting skin, empirical tests:Graft survival rate be up to 80% with On, be conducive to the further raising of Magnolia wufengensis nursery stock spring grafting technology to develop, while saving grafting cost, improving grafting side Method, plays an important roll to amount reproduction Magnolia wufengensis this rare variety of nursery stock spring.
Method two
Magnolia wufengensis spring, embedding bud cutting skin engrafting method also included:During grafting after scion insertion stock, sprayed with exocellular polysaccharide It is coated in the surface of scion and stock joint.
Exocellular polysaccharide, be some peculiar microorganisms be secreted into that cell membrane is outer during growth metabolism, easily separated with thalline, The water-soluble polysaccharide in environment is secreted into, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide (exopolysaccharides, EPS):It is that some peculiar microorganisms are secreted during growth metabolism Water-soluble polysaccharide that is outer to cell membrane, easily being separated with thalline, be secreted into environment, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide microbial polysaccharide compared with plant polyose has unique and excellent physical characteristic, such as in low concentration Under, viscosity can be increased, without forming gel;Small shearforce, can quickly increase mobility, and reduce viscosity, as long as Shearforce is eliminated, can extensive revival rapidly.The glucan produced by leuconostoc dextranicum bacteria can as column material, big point Sub- modifying agent etc..
Experiment:
Above engrafting method one (experimental group 1), method two (experimental group 2) is (right with traditional engrafting method simple bud cutting method 1) and with wooden cortex grafting (control group 2) Experimental comparison is carried out according to group.Each experimental group randomly chooses three skills involved in the labour one The grafting workman of cause carries out grafting to 100 plants of Magnolia wufengensis, so that by respective grafting situation to three kinds of survival rates of method It is compared with the labor intensive time.It is as shown in the table:
Experimental group 1 Experimental group 2 Control group 1 Control group 2
Grafting quantity 100 100 100 100
Survive quantity 80 82 15 10
Survival rate 80% 82% 15% 10%
The consuming time/h 2.1 2.2 2 2
Test result indicate that:By this graft technology inventive method, one is that to belong to 1 year raw Magnolia wufengensis nursery stock new due to yulan Branch, vitality, growing way are strong, easily form callus;Two is that scion dormancy is hidden into bud, the formation of the embedding bud cutting skin of cut-grafting and stock Layer laminating closely, parenchyma cell is adjacent to, and joint is closely sealed, produces the ability of callus strong between stock and scion, is easily formed The materials such as new conducting tissue, moisture and nutrition can be transported in place;Three is that Magnolia wufengensis nursery stock spring grafting mouthful faces south, just In the position daylighting of grafting bud, the favourable embedding bud cutting skin of grafting and stock heal.80% survival is can reach using this kind of engrafting method Rate, far above the survival rate of two other control group 10% and 15%.Therefore this grafting invention solves Magnolia wufengensis well The survival rate of nursery stock spring grafting, this grafting and breeding to Magnolia wufengensis nursery stock spring has material impact.
Experimental group 2, using exocellular polysaccharide plant surface formed viscosity, without formed gel the characteristics of, can improve Scion and stock connective stability, can also fill the space of special grafting membrane and plant surface, again will not be to plant growth shape Into obstruction, so as to further increase plant survival rate.
The above is only the preferred embodiment of this graft technology invention, protection scope of the present invention should be with claim institute The scope of restriction is defined.For those skilled in the art, without departing from the spirit and scope of the present invention Can also make it is some improvement and it is perfect, these improve and improve should also be considered as protection scope of the present invention.

Claims (6)

1. embedding bud cutting skin engrafting method of a kind of Magnolia wufengensis nursery stock spring, it is characterised in that:The previous year growth is belonged to yulan vigorous Shoot be stock, with Magnolia wufengensis current-year branch as scion, 2 months first arrival late March carry out grafting work, all grafting works Work must be completed before stock rudiment, and grafting temperature is adapted at 4-18 DEG C along the Changjiang river, and northern grafting temperature is adapted at 8-18 DEG C;
Hide bud:During winter dormancy is entered, first Magnolia wufengensis current-year branch is cut as scion, load cloth bag or gunnysack by its Below 1 meter or so of underground pit excavation of embedment or frozen soil layer, first layer overlay 30cm is thick husky again with earthen backfill covering, is taken when grafting is needed Go out;
Stock:Selection the previous year, eugonic shoot was stock, was close to the angular cut of inside cutting one of bast in stock, then From top from top to bottom vertical sliding cutting to forming a block length tangent plane at angular cut;
Cut embedding bud cutting skin:Knife face is cut into 180 ° of planes at the embedding bud cutting skin side 0.8-1cm of scion;
Grafting:The embedding bud cutting skin of scion after cutting is docked in the forming layer of both stock tangent planes long, its identical rate is reached hundred Divide hundred, so as to form grafting;
Sealer:The embedding bud cutting skin of scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to swathe tight Can not intake in fact, bud-leaf exposes;
Cut anvil:Sprouting after to next year grafting is long to 15cm, and stock on bud is all cut.
2. Magnolia wufengensis nursery stock spring according to claim 1 embedding bud cutting skin engrafting method, it is characterised in that the grafting Method choice is sunny or cloudy weather is carried out.
3. Magnolia wufengensis nursery stock spring according to claim 1 embedding bud cutting skin engrafting method, it is characterised in that stock is long to be cut The a length of 4-5cm in face, a width of 0.5-1.5cm;The embedding a length of 3-5cm of bud cutting skin of scion.
4. Magnolia wufengensis nursery stock spring according to claim 1 embedding bud cutting skin engrafting method, it is characterised in that:The Changjiang river Grafting temperature is at 6 ~ 15 DEG C along the line.
5. Magnolia wufengensis nursery stock spring according to claim 1 embedding bud cutting skin engrafting method, it is characterised in that:Northern grafting Temperature is at 8 ~ 15 DEG C.
6. Magnolia wufengensis nursery stock spring according to claim 1 embedding bud cutting skin engrafting method, it is characterised in that:During grafting, After scion insertion stock, the surface of scion and stock joint is sprayed on exocellular polysaccharide.
CN201710048461.XA 2017-01-22 2017-01-22 Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring Expired - Fee Related CN106856995B (en)

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CN201710048461.XA CN106856995B (en) 2017-01-22 2017-01-22 Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710048461.XA CN106856995B (en) 2017-01-22 2017-01-22 Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring

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CN106856995B CN106856995B (en) 2020-01-07

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103694049A (en) * 2013-12-20 2014-04-02 广西大学 Plant protection product
CN104126418A (en) * 2014-07-08 2014-11-05 安徽美心生态园林有限公司 Southern magnolia grafting method
CN104206183A (en) * 2014-08-06 2014-12-17 金寨县万紫千红农业科技开发有限公司 Method for grafting magnolia liliiflora to michelia alba
CN104996171A (en) * 2015-07-08 2015-10-28 北京林业大学 High position grafting method for magnolia wufengensis
CN105009946A (en) * 2015-07-08 2015-11-04 北京林业大学 Grafting method of Magnolia wufengensis
CN105052569A (en) * 2015-09-22 2015-11-18 江苏农林职业技术学院 Method for realizing shield bud grafting with wood of seedling

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103694049A (en) * 2013-12-20 2014-04-02 广西大学 Plant protection product
CN104126418A (en) * 2014-07-08 2014-11-05 安徽美心生态园林有限公司 Southern magnolia grafting method
CN104206183A (en) * 2014-08-06 2014-12-17 金寨县万紫千红农业科技开发有限公司 Method for grafting magnolia liliiflora to michelia alba
CN104996171A (en) * 2015-07-08 2015-10-28 北京林业大学 High position grafting method for magnolia wufengensis
CN105009946A (en) * 2015-07-08 2015-11-04 北京林业大学 Grafting method of Magnolia wufengensis
CN105052569A (en) * 2015-09-22 2015-11-18 江苏农林职业技术学院 Method for realizing shield bud grafting with wood of seedling

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
蔡冬元: "《苗木生产技术》", 31 August 2012 *

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Address after: No.22, Renheping Town, Wufeng Tujia Autonomous County, Yichang City, Hubei Province

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Patentee after: YICHANG FOREST TREE SEEDLING MANAGEMENT STATION

Address before: 443000 rooms 401-405, block a, modern city square, Yichang City, Hubei Province

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