CN106856995B - Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring - Google Patents
Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring Download PDFInfo
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- CN106856995B CN106856995B CN201710048461.XA CN201710048461A CN106856995B CN 106856995 B CN106856995 B CN 106856995B CN 201710048461 A CN201710048461 A CN 201710048461A CN 106856995 B CN106856995 B CN 106856995B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G3/00—Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
- A01G2003/005—Removing buds
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a bud-embedded bark-inserted grafting method for magnolia sieboldii seedlings in spring, which takes magnolia seedlings as stocks, annual branches of magnolia sieboldii as scions, and carries out grafting work in the middle ten days of 2 months to 3 months, wherein the grafting temperature along the Yangtze river is suitable for 4-18 ℃, the grafting temperature in the north is suitable for 8-18 ℃, and the grafting process comprises six steps of bud hiding, stock cutting, bud cutting, grafting, film winding and stock cutting. The grafting method has the advantages that the survival rate can reach more than 80%, the growth height in the current year after survival can reach more than 1.5 m, and the method for grafting the magnolia denudata seedlings by bud insertion and bark cutting solves the problem of extremely low survival rate of the magnolia denudata seedlings in spring grafting.
Description
Technical Field
The invention belongs to the field of seedling propagation, relates to a grafting method of plants, and particularly relates to a spring bud-embedding bark-inserting grafting method of magnolia denudata seedlings.
Background
Magnolia grandiflora (Magnolia wufengensis), also known as Magnolia wufengensis, is a new species of Magnoliaceae plants discovered by professor masson university of Beijing forestry university in 2004 when Magnoliaceae plant resource investigation was performed in Wufeng, only in Wufeng of China. The magnolia denudata belongs to a tall tree, is beautiful in tree shape, has extremely rich morphological variation characteristics of flower parts, has the number of flower quilt pieces from 9 petals to 46 petals, has different expressions of deep red, red and light red in flower color types, has extremely high ornamental value, and is an excellent tree species for garden greening.
At present, magnolia sieboldii is not completely developed in the landscaping market, and the propagation survival rate is low, so that the survival rate of grafted seedlings is greatly improved by adopting a cleft grafting and head changing cutting grafting method.
Disclosure of Invention
The invention aims to provide a grafting method for grafting magnolia denudata seedlings with buds embedded and peels inserted in spring, wherein the survival rate of the magnolia denudata seedlings reaches 80%, the grafting survival rate is high, and the grafting conditions are strict.
In order to solve the technical problems, the technical scheme provided by the invention is that a method for grafting magnolia denudata seedlings by bud-embedding and bark-inserting in spring is provided, a new branch growing vigorously in the previous year of magnolia is taken as a stock, the current-year branch of magnolia denudata is taken as a scion, grafting work is carried out in the first ten to 3 months of the month, all grafting work is required to be completed before the stock sprouts, the grafting temperature along the Yangtze river is suitable at 4-18 ℃, and the grafting temperature in the north is suitable at 8-18 ℃;
tibetan bud: during hibernation in winter, firstly, cutting off current-year-old branches of magnolia denudata serving as scions, filling the scions into a cloth bag or a gunny bag, burying the cloth bag or the gunny bag into an underground pit with the depth of about 1 m or below a frozen soil layer, firstly, paving a layer of sand with the thickness of 30cm, then backfilling the soil to cover the pit, and taking out the scions when grafting is needed;
stock: selecting a new branch growing vigorously in the previous year as a stock, cutting a bevel cut on the inner side of the stock close to the phloem, and vertically and slidably cutting the stock from top to bottom to the bevel cut to form a long cut;
cutting, embedding and bud cutting and skin inserting: cutting the scion bud-embedded cutting skin at a knife face of 180 degrees at a position of 0.8-1cm of the lateral surface of the scion bud-embedded cutting skin;
grafting: the cut scion bud-embedded bark is butted with the cambium of the long cut surface of the stock, so that the coincidence rate reaches hundreds, and grafting is formed;
sealing the film: binding the grafting bud-embedded grafting bark of the scion and the butt joint of the stock from bottom to top by using a special grafting film, wherein the grafting bud-embedded grafting bark is required to be tightly wound and water cannot enter, and bud leaves are exposed;
cutting an anvil: and (4) until the new bud grows to 15cm after the next year of grafting, and all the rootstocks on the bud are removed.
Further, the grafting method is carried out in sunny or cloudy weather.
Further, the length of the stock is 4-5cm, and the width of the stock is 0.5-1.5 cm; the length of the scion bud-embedding cutting skin is 3-5 cm.
Further, the grafting temperature along the Yangtze river is 6-15 ℃.
Furthermore, the grafting temperature in the north is 8-15 ℃.
Furthermore, during grafting, after the scion is inserted into the stock, extracellular polysaccharide is sprayed on the surface of the butt joint of the scion and the stock.
Compared with the prior art, the grafting technology has the following advantages:
1. the grafting survival rate is up to more than 80 percent, which is beneficial to further improving and developing the spring grafting technology of the magnolia sieboldii seedlings and perfecting the grafting method;
2. can enhance the disease resistance and low temperature resistance of plants, solves the problem that the traditional magnolia sieboldii grafting only can be in autumn range and capacity, and plays an important role in propagating magnolia sieboldii, a rare variety in large quantities.
Detailed Description
The following description is made with reference to specific examples. The present examples are intended to illustrate the principles and features of the present invention, and are intended to be illustrative of the present technology and not limiting in scope.
Method 1
A bud-embedded and bark-inserted grafting method for magnolia sieboldii seedlings in spring takes new branches growing vigorously in the first year of magnolia as rootstocks, branches growing vigorously in the current year of magnolia sieboldii as scions, grafting is carried out in the first ten days to the next 3 months in 2 months, the grafting temperature along the Yangtze river is 6-15 ℃, all grafting works must be guaranteed to be completed before the rootstocks sprout, the problem of extremely low survival rate of magnolia sieboldii seedlings in spring grafting is solved by the bud-embedded and bark-inserted grafting method for magnolia sieboldii seedlings in spring grafting, and the blank is filled in the grafting work of magnolia sieboldii seedlings in spring by adopting the grafting method.
The grafting method for the magnolia denudata bud-embedding and bark-inserting in spring in the embodiment specifically comprises the following steps:
1) tibetan bud: during hibernation in winter, the current annual branches (namely scions) of the magnolia sieboldii are cut off, the magnolia sieboldii is put into a cloth bag or a gunny bag and buried in an underground pit for about 1 m (below a frozen soil layer), a layer of sand with the thickness of 30cm is paved, then the ground is backfilled and covered, and the magnolia sieboldii is taken out when grafting is needed.
2) Stock: firstly, selecting a new branch growing vigorously in the previous year as a stock, cutting a bevel cut on the inner side of the stock close to the phloem, and vertically and slidably cutting the stock from top to bottom to the bevel cut to form a long section.
3) Cutting, embedding and bud cutting and skin inserting: cutting the scion bud-embedded cutting skin at a position of 0.8-1cm away from the side surface of the scion bud-embedded cutting skin by a plane of 180 degrees.
4) Grafting: and (3) carrying out butt joint on the cut scion bud-embedded bark and the root stock long section to enable the coincidence rate to reach hundreds, thereby forming grafting.
5) Sealing the film: binding the grafting bud-embedded grafting bark of the scion and the butt joint of the stock from bottom to top by using a special grafting film, wherein the grafting bud-embedded grafting bark is required to be tightly wound and water cannot enter, and bud leaves are exposed.
6) Cutting an anvil: and (4) until the new bud grows to 15cm after the next year of grafting, and all the rootstocks on the bud are removed.
The grafting method for the magnolia sieboldii seedlings is characterized in that the magnolia sieboldii seedlings are cut and grafted with bud and bark in spring through the method, and the method is proved to be as follows: the grafting survival rate is up to more than 80%, the further improvement and development of the spring grafting technology of the magnolia denudata seedlings are facilitated, the grafting cost is saved, the grafting method is perfected, and the method plays an important role in mass propagation of the magnolia denudata seedlings as a rare variety in spring.
Method two
The grafting method of the magnolia denudata by bud-embedding and bark-inserting in spring also comprises the following steps: when grafting, the scion is inserted into the stock and then is sprayed on the surface of the butt joint of the scion and the stock by exopolysaccharide.
The extracellular polysaccharide is a water-soluble polysaccharide secreted outside a cell wall, easily separated from thalli and secreted into the environment in the growth and metabolism process of some special microorganisms, and belongs to a secondary metabolite of the microorganisms.
Exopolysaccharides (EPS): is a water-soluble polysaccharide secreted outside a cell wall by some special microorganisms in the growth and metabolism process, is easy to separate from thalli and is secreted into the environment, and belongs to a secondary metabolite of the microorganisms.
Exopolysaccharides have unique and superior physical properties compared to plant polysaccharides, such as increased viscosity at low concentrations without gel formation; the micro shear force can quickly increase the fluidity and reduce the viscosity, and the original effect can be quickly recovered as long as the shear force is eliminated. The dextran produced by the dextran leuconostoc can be used as chromatographic column materials, macromolecular modifiers and the like.
Experiment:
the above grafting methods one (experimental group 1) and two (experimental group 2) were compared with the conventional grafting method single bud cutting method (control group 1) and grafting with wood bark (control group 2). Three grafting workers with consistent technical proficiency are randomly selected for each experimental group to graft 100 magnolia sieboldii plants, so that the survival rate and the labor time consumption of the three methods are compared according to respective grafting conditions. As shown in the following table:
| experimental group 1 | Experimental group 2 | Control group 1 | Control group 2 | |
| Number of grafts | 100 | 100 | 100 | 100 |
| Number of survivals | 80 | 82 | 15 | 10 |
| Survival rate | 80％ | 82％ | 15％ | 10％ |
| Takes time/h | 2.1 | 2.2 | 2 | 2 |
The experimental results show that: through the method of the invention of the grafting technology, firstly, because the magnolia belongs to the new branch of the magnolia sieboldii seedling of the 1-year-old magnolia sieboldii, the vitality and the growth vigor are strong, and the healing tissue is easy to form; secondly, the dormant bud hiding of the scion, the cut bud embedding and inserting skin is closely attached to the cambium of the stock, the thin-wall cells are closely attached, the junction is closed, the capability of generating a healing tissue between the stock and the scion is strong, a new conduction tissue is easy to form, and substances such as moisture, nutrition and the like can be conveyed in place; and thirdly, the grafting opening of the magnolia denudata seedling in spring is exposed to the sun, so that the lighting of the grafted bud part is facilitated, and grafting, bud-embedding and bark-inserting and stock healing are facilitated. The survival rate of 80% can be reached by using the grafting method, which is far higher than that of the other two control groups by 10% and 15%. Therefore, the grafting method well solves the problem of the survival rate of the magnolia sieboldii seedlings grafted in spring, and has important influence on the grafting and breeding of the magnolia sieboldii seedlings in spring.
The experiment group 2 utilizes the characteristic that extracellular polysaccharide forms viscosity on the surface of a plant and does not form gel, can improve the connection stability of the scion and the stock, can fill a gap between a special grafting film and the surface of the plant, and cannot form obstruction to the growth of the plant, thereby further improving the survival rate of the plant.
The above is only a preferred embodiment of the present invention, and the protection scope of the present invention shall be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and scope of the invention, and these modifications and improvements should be considered to be within the scope of the invention.
Claims (5)
1. A bud-embedded bark-inserted grafting method for magnolia sieboldii seedlings in spring is characterized by comprising the following steps: taking a new branch growing vigorously in the previous year of the magnolia as a stock, taking an annual branch of the magnolia sieboldii as a scion, and carrying out grafting work from the beginning of 2 months to the late 3 months, wherein all grafting work needs to be finished before the stock sprouts, the grafting temperature along the Yangtze river is suitable at 4-18 ℃, and the grafting temperature in the north is suitable at 8-18 ℃;
tibetan bud: during hibernation in winter, firstly, cutting off current-year-old branches of magnolia denudata serving as scions, filling the scions into a cloth bag or a gunny bag, burying the cloth bag or the gunny bag into an underground pit with the depth of about 1 m or below a frozen soil layer, firstly, paving a layer of sand with the thickness of 30cm, then backfilling the soil to cover the pit, and taking out the scions when grafting is needed;
stock: selecting a new branch growing vigorously in the previous year as a stock, cutting a bevel cut on the inner side of the stock close to the phloem, and vertically and slidably cutting the stock from top to bottom to the bevel cut to form a long cut;
cutting, embedding and bud cutting and skin inserting: cutting the scion bud-embedded cutting skin at a knife face of 180 degrees at a position of 0.8-1cm of the lateral surface of the scion bud-embedded cutting skin;
grafting: the cut scion bud-embedded bark is butted with the cambium of the long cut surface of the stock, so that the coincidence rate reaches hundreds, and grafting is formed;
sealing the film: binding the grafting bud-embedded grafting bark of the scion and the butt joint of the stock from bottom to top by using a special grafting film, wherein the grafting bud-embedded grafting bark is required to be tightly wound and water cannot enter, and bud leaves are exposed;
cutting an anvil: until the new bud grows to 15cm after the next year of grafting, the rootstocks on the bud are completely removed;
during grafting, after the scion is inserted into the stock, extracellular polysaccharide is sprayed on the surface of the butt joint of the scion and the stock.
2. The grafting method for the magnolia sieboldii seedlings with bud-embedding and bark-inserting functions in spring according to claim 1, wherein the grafting method is performed in sunny or cloudy weather.
3. The grafting method of the magnolia sieboldii seedlings with bud-embedding and bark-inserting in spring according to claim 1, wherein the length of the long cut surface of the rootstock is 4-5cm, and the width is 0.5-1.5 cm; the length of the scion bud-embedding cutting skin is 3-5 cm.
4. The grafting method for the magnolia sieboldii seedlings with bud embedding and bark cutting in spring according to claim 1, wherein the grafting temperature along the Yangtze river is 6 ~ 15 ℃.
5. The grafting method for the magnolia sieboldii seedlings with bud embedding and bark cutting in spring according to claim 1, wherein the grafting temperature in the north is 8 ~ 15 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710048461.XA CN106856995B (en) | 2017-01-22 | 2017-01-22 | Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring |
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| CN201710048461.XA CN106856995B (en) | 2017-01-22 | 2017-01-22 | Bud-embedding and bark-inserting grafting method for magnolia sieboldii seedlings in spring |
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| CN106856995B true CN106856995B (en) | 2020-01-07 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103694049A (en) * | 2013-12-20 | 2014-04-02 | 广西大学 | Plant protection product |
| CN104126418A (en) * | 2014-07-08 | 2014-11-05 | 安徽美心生态园林有限公司 | Southern magnolia grafting method |
| CN104206183A (en) * | 2014-08-06 | 2014-12-17 | 金寨县万紫千红农业科技开发有限公司 | Method for grafting magnolia liliiflora to michelia alba |
| CN104996171A (en) * | 2015-07-08 | 2015-10-28 | 北京林业大学 | High position grafting method for magnolia wufengensis |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105052569A (en) * | 2015-09-22 | 2015-11-18 | 江苏农林职业技术学院 | Method for realizing shield bud grafting with wood of seedling |
-
2017
- 2017-01-22 CN CN201710048461.XA patent/CN106856995B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103694049A (en) * | 2013-12-20 | 2014-04-02 | 广西大学 | Plant protection product |
| CN104126418A (en) * | 2014-07-08 | 2014-11-05 | 安徽美心生态园林有限公司 | Southern magnolia grafting method |
| CN104206183A (en) * | 2014-08-06 | 2014-12-17 | 金寨县万紫千红农业科技开发有限公司 | Method for grafting magnolia liliiflora to michelia alba |
| CN104996171A (en) * | 2015-07-08 | 2015-10-28 | 北京林业大学 | High position grafting method for magnolia wufengensis |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105052569A (en) * | 2015-09-22 | 2015-11-18 | 江苏农林职业技术学院 | Method for realizing shield bud grafting with wood of seedling |
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Address after: No.22, Renheping Town, Wufeng Tujia Autonomous County, Yichang City, Hubei Province Patentee after: ZHONGSEN Corp. Patentee after: YICHANG FOREST TREE SEEDLING MANAGEMENT STATION Address before: 443000 rooms 401-405, block a, modern city square, Yichang City, Hubei Province Patentee before: ZHONGSEN Corp. Patentee before: YICHANG FOREST TREE SEEDLING MANAGEMENT STATION |
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Granted publication date: 20200107 Termination date: 20220122 |