CN106804275A - A kind of method for culturing seedlings of purple perilla - Google Patents

A kind of method for culturing seedlings of purple perilla Download PDF

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Publication number
CN106804275A
CN106804275A CN201710127097.6A CN201710127097A CN106804275A CN 106804275 A CN106804275 A CN 106804275A CN 201710127097 A CN201710127097 A CN 201710127097A CN 106804275 A CN106804275 A CN 106804275A
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China
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purple perilla
seed
seedbed
culturing seedlings
seedling
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CN201710127097.6A
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Chinese (zh)
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翟洪平
刘荣贵
许志祥
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Ecological Agricultural Science And Technology Development Co Ltd Of Guizhou
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Ecological Agricultural Science And Technology Development Co Ltd Of Guizhou
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention belongs to seedling fostering technical field, more particularly to a kind of method for culturing seedlings of purple perilla;Comprise the following steps:Seeds preprocess, build seedbed, field management;The method that the inventive method takes seeding and seedling raising, it is effectively promoted the differentiation of seed coat cell growth, shorten growing-seedling period, improve the resistance and the adaptive faculty to environment of purple perilla seedling, improve survival rate, using breeding method of the invention, the survival rate of purple perilla seedling is up to more than 90%, compared to conventional breeding processes, survival rate improves 5~10.1%, per mu yield after the purple perilla harvesting that the present invention is cultivated is in 1728.3~1745.9kg, the per mu yield of the purple perilla planted relative to conventional method, it is capable of achieving volume increase 25.6~26.9%, effectively improve the per mu yield of purple perilla.

Description

A kind of method for culturing seedlings of purple perilla
Technical field
The invention belongs to seedling fostering technical field, more particularly to a kind of method for culturing seedlings of purple perilla.
Background technology
Purple perilla, alias:Common perilla, red Soviet Union etc.;It is Labiatae annual herb plant, with special fragrance, is wrinkled blade more Crinkle song, is in oval after complete person's flattening, long 4 to 11 centimetres, wide 2.5 to 9 centimetres, and tip is long sharp or anxious sharp, basal circular Or wide wedge shape, edge tool crenature, two sides purple or green above, lower surface has most concave point shape glandular scales, and petiole is long 2 to 5 lis Rice, purple or purple green, matter are crisp, the purple green of spray, and there are marrow, gas delicate fragrance, taste micro-pungent in section middle part.
Purple perilla is the traditional medicine edible oil material dual-purpose crop of China, and Caulis Perillae, leaf, seed are traditional Chinese medicines, are health ministry First batch of 60 chromium assert be both medicine one of the medicine-food two-purpose crop of food again, perilla leaf is a kind of in southern china Zhanjiang Wu The widely used delicious flavouring in valley area, people are usually cooked with its leaf, and its delicious food is often mutually carried with clam beach wormwood Mention in the same breath, purple perilla oil yield is up to 45~55%, and unrighted acid accounts for more than the 90% of total oil mass, and wherein alpha-linolenic acid content is high It is the important necessary aliphatic acid of human body up to 50~70%, d- leukotrienes, alpha-linolenic acid is w-3 series of height unrighted acids Parent, in vivo metabolism can be converted into DHA (docosapentaenoic acid), EPA (blood vessel scavenger), DPA (shield brain expert), with promotion brain Development, improve memory, improve intelligence and eyesight, adjust immunity, reduce cholesterol and blood fat, anti-aging, by liver protection, The health-care efficacies such as cancer-resisting, hypo-allergenic and full nutrition.
In the prior art, it is uneven on purple perilla seedling raising mannerses, and processing mode is incorrect, causes the survival of purple perilla seedling Rate is relatively low, and growth situation is poor, and resistance is poor, therefore, to meet great demand of the existing market to purple perilla, improve purple perilla The rational seedling raising mannerses of purple perilla design science are had important practice significance and economic worth by economic worth.
The content of the invention
In order to solve the above-mentioned technical problem, the present invention provides a kind of method for culturing seedlings of purple perilla.
Specifically it is realized in:
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:Full grains, the purple perilla seed that has no mechanical damage are chosen, first 30 are soaked with pretreatment fluid~ 35min, seed is pulled out and is drained, then purple perilla seed is placed in into 30~40 DEG C, and relative humidity is that in the environment of 50~55%, will plant Son tiling, is covered with towel, by the standard of 500~550m1/m2, a growth stimulator is sprayed every 2 hours, after 2d, seed is taken Go out, seed is dressed seed with medicament, wherein seed and medicament compare mixing for standby use by 1: 1.8~2.2 quality;By pretreatment The immersion of liquid, is conducive to killing the worm's ovum for being attached to the surface of the seed, lifts the quality of seed, is that the growth situation of later stage purple perilla is carried For basic guarantee, vernalization treatment is carried out to seed in certain circumstances, by spraying for growth stimulator, promote seed coat tissue Differentiation and growth, promote rudiment, shorten growth cycle, it is ensured that the quality of growth, and the seed and medicament after vernalization are dressed seed, Both can guarantee that seed in soil from the invasion of pest and disease damage and underground worm's ovum, it is ensured that the yield and quality of purple perilla.
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 120~150kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can Start sowing;By the addition of modifying agent, the fertility of soil can be effectively improved, for purple perilla growth provides endlessly nutrition Supply, while, moreover it is possible to improve the oxygen content in soil, improve gas permeability when purple perilla grows, promote growing for purple perilla.
(3) field management:After planting seedbed is watered, mu irrigation amount is 80~100kg, until seed sprouting, During this keep nursery bed temperature be 24.5~26.5 DEG C, relative humidity be 25~35%, whne right seedling it is long to 5~8cm when, to seedbed Spray accelerator, 27.5~28.5 DEG C are risen to by temperature, and relative humidity is 20~22%, and period carries out artificial weeding to seedbed, Treat that seedling is long to be transplanted to by more than 20cm.During nursery, nursery bed temperature and relative humidity are controlled, in suitable environment Under, the growth of seed is more beneficial for, promote the differentiation of epidermal cell, shorten growing-seedling period, meanwhile, accelerator is sprayed to crop, Further meet the nutritional need of purple perilla growth, shorten growing-seedling period.
Further, described pretreatment fluid, be by 0.5~0.7% hydrogen peroxide, 12~15% alcohol and 0.25~ 0.28% physiological saline is mixed by 1~1.5: 0.8~1.2: 2~3 mass ratio, ultrasonically treated 3~5min, wherein in advance The temperature for the treatment of fluid is 25~27 DEG C.By setting suitable concentration and proportioning, the infringement by pretreatment fluid to seed is dropped to most It is low, pretreatment fluid is performed to the insect killing effect of the worm's ovum bacterium of seed coat best, it is ensured that the safety and later stage kind of seed Plant property.
Further, described growth stimulator, is made up of following raw material by weight:Nafusaku 15~20, vitamin C11~15, pomelo peel extract solution 20~30, Chinese medicine extract 20~30, trace element 8~12, biogas slurry 20~25, dregs of beans 3~ 5th, stalk 4~6, animal human bones 1.2~2.5.
Further, described growth stimulator, its preparation method, comprise the following steps:
(1) be by dregs of beans, stalk and animal human bones crush after, mix with biogas slurry, be placed in fermenting cellar, with 0.5~0.8 DEG C/ Temperature is risen to 50~55 DEG C by the speed of min, and relative humidity is 25~30%, after 12~15h of fermentation, cools the temperature to 25~30 DEG C, relative humidity is 45~55%, and ferment 6~8h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, 1~1.2h is stirred in water bath with thermostatic control at 30~35 DEG C, Filtering, takes filtrate, puts into watering can.
Further, described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water is to white flesh in wadding Shape, filtering is concentrated into the 1/3 of filtrate content, adds 4~5h of immersion that concentration is 75% ethanol, reclaims ethanol, adds acetic acid second Ester, is heated to reflux more than 2 times, and 20~25min, reclaims ethyl acetate every time, you can.
Further, described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 15~22, akebi 2 ~3, lophatherum gracile 20~25, radix stellariae dichotomae 6~10, caulis lonicerae 12~18, Caulis Perillae 15~19, the root bark of Chinese wolf-berry 18~22, Chinese violet 18~22.
Further, described medicament, is made up of following raw material by weight:50% captan 1.5~2,75,%00 It is bacterium clear 2~3, S-Ethyl ethylthio sulfonate 5~8, capsule of weeping forsythia powder 10~15, stone-like omphalia powder 4~7, humic sour soil 20~30, nitrogen-fixing bacteria 1.2~1.5, withered Careless bacillus 1.8~2.2.
Further, described modifying agent, is made up of following raw material by weight:Perlite 10~20, waste mushroom stick 15 ~20, husk 6~10, river bottom mud 12~18, tealeaf residue 8~15.
Further, described accelerator, urea 20~30, potassium dihydrogen phosphate 18~25, potassium sulfate 10~20, vitamin A2.5~3.5, vitamin C 3~4, glycine 0.25~0.35, rhizoma alismatis 8~12, Lygodium japonicum 10~15, rde bean 8~12, crane Careless bud 3~4, Ramulus Taxilli 1~2.
In sum, the beneficial effects of the present invention are:The method that the inventive method takes seeding and seedling raising, is effectively promoted Seed coat cell growth is broken up, and shortens growing-seedling period, improves the resistance and the adaptive faculty to environment of purple perilla seedling, is improved Survival rate, using breeding method of the invention, the survival rate of purple perilla seedling up to more than 90%, compared to conventional breeding processes, Survival rate improve 5~10.1%, the present invention cultivate purple perilla harvesting after per mu yield in 1728.3~1745.9kg, relative to biography The per mu yield of the purple perilla of system method plantation, is capable of achieving volume increase 25.6~26.9%, effectively improves the per mu yield of purple perilla.
By the immersion of pretreatment fluid, be conducive to killing the worm's ovum for being attached to the surface of the seed, the quality of seed lifted, after being The growth situation of phase purple perilla provides basic guarantee, carries out vernalization treatment to seed in certain circumstances, by spraying for growth stimulator, Promote the differentiation and growth of seed coat tissue, promote rudiment, shorten growth cycle, purple perilla root growth is more sent out Up to the quality for ensureing growth, the seed and medicament after vernalization are dressed seed, both can guarantee that seed in soil from pest and disease damage With the invasion of underground worm's ovum, it is ensured that the yield and quality of purple perilla.
By the addition of modifying agent, the fertility of soil can be effectively improved, for purple perilla growth provides endlessly nutrition Supply, while, moreover it is possible to improve the oxygen content in soil, improve gas permeability when purple perilla grows, promote growing for purple perilla.
During nursery, nursery bed temperature and relative humidity are controlled, under suitable environment, are more beneficial for the life of seed It is long, promote the differentiation of epidermal cell, shorten growing-seedling period, meanwhile, accelerator is sprayed to crop, further meet purple perilla growth Nutritional need, shortens growing-seedling period.
Specific embodiment
Specific embodiment of the invention is described in further detail below, but the invention is not limited in these realities Mode is applied, any improvement or replacement on the present embodiment essence spirit still falls within the claims in the present invention required for protection Scope.
Embodiment 1
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:The purple perilla seed for choose full grains, having no mechanical damage, is first soaked with pretreatment fluid 30min, seed is pulled out and is drained, then purple perilla seed is placed in into 30 DEG C, and relative humidity is in the environment of 50%, seed to be tiled, Covered with towel, by the standard of 500m1/m2, a growth stimulator was sprayed every 2 hours, after 2d, seed taken out, with medicament pair Seed is dressed seed, and wherein seed and medicament compare mixing for standby use by 1: 1.8 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 120kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 80kg, until seed sprouting, during this Keep nursery bed temperature be 24.5 DEG C, relative humidity is 25%, whne right seedling it is long to 5cm when, to seedbed spray accelerator, by temperature liter To 27.5 DEG C, relative humidity is 20%, and period carries out artificial weeding to seedbed, treats that seedling is long and is transplanted to by more than 20cm.
Described pretreatment fluid, is by 1: 0.8 by 0.5% hydrogen peroxide, 12% alcohol and 0.25% physiological saline: 2 mass ratio mixing, the temperature of ultrasonically treated 3min, wherein pretreatment fluid is 25 DEG C.
Described growth stimulator, is made up of following raw material by weight:Nafusaku 15kg, vitamin C 11kg, shaddock Skin extract solution 20kg, Chinese medicine extract 20kg, trace element 8kg, biogas slurry 20kg, dregs of beans 3kg, stalk 4kg, animal human bones 1.2kg。
Described growth stimulator, its preparation method is comprised the following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.5 DEG C/min's Temperature is risen to 50 DEG C by speed, and relative humidity is 25%, after fermentation 12h, cools the temperature to 25 DEG C, and relative humidity is 45%, hair Ferment 6h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, water bath with thermostatic control stirring 1h, filtering, take filter at 30 DEG C Liquid, puts into watering can.
Described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water, in cotton-shaped, is filtered to white flesh, The 1/3 of filtrate content is concentrated into, the immersion 4h that concentration is 75% ethanol is added, ethanol is reclaimed, ethyl acetate is added, is heated to reflux More than 2 times, each 20min reclaims ethyl acetate, you can.
Described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 15kg, akebi 2kg, lophatherum gracile 20kg, radix stellariae dichotomae 6kg, caulis lonicerae 1kg, Caulis Perillae 15kg, root bark of Chinese wolf-berry 18kg, Chinese violet 18kg.
Described medicament, is made up of following raw material by weight:50% captan 1.5kg, 75% Bravo 2kg, S-Ethyl ethylthio sulfonate 5kg, capsule of weeping forsythia powder 10kg, stone-like omphalia powder 4kg, humic sour soil 20kg, nitrogen-fixing bacteria 1.2kg, bacillus subtilis 1.8kg.
Described modifying agent, is made up of following raw material by weight:Perlite 10kg, waste mushroom stick 15kg, husk 6kg, river bottom mud 12kg, tealeaf residue 8kg.
Described accelerator, urea 20kg, potassium dihydrogen phosphate 18kg, potassium sulfate 10kg, dehydroretinol .5kg, vitamin C3kg, glycine 0.25kg, rhizoma alismatis 8kg, Lygodium japonicum 10kg, rde bean 8kg, gemma agrimoninae 3kg, Ramulus Taxilli 1kg.
Embodiment 2
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:The purple perilla seed for choose full grains, having no mechanical damage, is first soaked with pretreatment fluid 35min, seed is pulled out and is drained, then purple perilla seed is placed in into 40 DEG C, and relative humidity is in the environment of 55%, seed to be tiled, Covered with towel, by the standard of 550m1/m2, a growth stimulator was sprayed every 2 hours, after 2d, seed taken out, with medicament pair Seed is dressed seed, and wherein seed and medicament compare mixing for standby use by 1: 2.2 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 150kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 100kg, until seed sprouting, this phase Between keep nursery bed temperature be 26.5 DEG C, relative humidity is 35%, whne right seedling it is long to 8cm when, to seedbed spray accelerator, by temperature 28.5 DEG C are risen to, relative humidity is 22%, period carries out artificial weeding to seedbed, treat that seedling is long and transplanted to by more than 20cm.
Described pretreatment fluid, is by 1.5 by 0.7% hydrogen peroxide, 15% alcohol and 0.28% physiological saline: 1.2: 3 mass ratio mixing, the temperature of ultrasonically treated 5min, wherein pretreatment fluid is 27 DEG C.
Described growth stimulator, is made up of following raw material by weight:Nafusaku 20kg, vitamin C 15kg, shaddock Skin extract solution 30kg, Chinese medicine extract 30kg, trace element 12kg, biogas slurry 25kg, dregs of beans 5kg, stalk 6kg, animal human bones 2.5kg。
Described growth stimulator, its preparation method is comprised the following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.8 DEG C/min's Temperature is risen to 55 DEG C by speed, and relative humidity is 30%, after fermentation 15h, cools the temperature to 30 DEG C, and relative humidity is 55%, hair Ferment 8h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, water bath with thermostatic control stirring 1.2h, filtering, take at 35 DEG C Filtrate, puts into watering can.
Described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water, in cotton-shaped, is filtered to white flesh, The 1/3 of filtrate content is concentrated into, the immersion 5h that concentration is 75% ethanol is added, ethanol is reclaimed, ethyl acetate is added, is heated to reflux More than 2 times, each 25min reclaims ethyl acetate, you can.
Described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 22kg, akebi 3kg, lophatherum gracile 25kg, radix stellariae dichotomae 10kg, caulis lonicerae 18kg, Caulis Perillae 19kg, root bark of Chinese wolf-berry 22kg, Chinese violet 22kg.
Described medicament, is made up of following raw material by weight:50% captan 2kg, 75% Bravo 3kg, second Allicin 8kg, capsule of weeping forsythia powder 15kg, stone-like omphalia powder 7kg, humic sour soil 30kg, nitrogen-fixing bacteria 1.5kg, bacillus subtilis 2.2kg.
Described modifying agent, is made up of following raw material by weight:Perlite 20kg, waste mushroom stick 20kg, husk 10kg, river bottom mud 18kg, tealeaf residue 15kg.
Described accelerator, urea 30kg, potassium dihydrogen phosphate 25kg, potassium sulfate 20kg, 3-Hydroxyretinol .5kg, vitamin C4kg, glycine 0.35kg, rhizoma alismatis 12kg, Lygodium japonicum 15kg, rde bean 12kg, gemma agrimoninae 4kg, Ramulus Taxilli 2kg.
Embodiment 3
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:The purple perilla seed for choose full grains, having no mechanical damage, is first soaked with pretreatment fluid 33min, seed is pulled out and is drained, then purple perilla seed is placed in into 35 DEG C, and relative humidity is in the environment of 53%, seed to be tiled, Covered with towel, by the standard of 530m1/m2, a growth stimulator was sprayed every 2 hours, after 2d, seed taken out, with medicament pair Seed is dressed seed, and wherein seed and medicament compare mixing for standby use by 1: 2 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 130kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 90kg, until seed sprouting, during this Keep nursery bed temperature be 25 DEG C, relative humidity is 30%, whne right seedling it is long to 7cm when, to seedbed spray accelerator, temperature is risen to 28 DEG C, relative humidity is 21%, and period carries out artificial weeding to seedbed, treats that seedling is long and is transplanted to by more than 20cm.
Described pretreatment fluid, is by 1.3: 1 by 0.6% hydrogen peroxide, 13% alcohol and 0.27% physiological saline: 2.5 mass ratio mixing, the temperature of ultrasonically treated 4min, wherein pretreatment fluid is 26 DEG C.
Described growth stimulator, is made up of following raw material by weight:Nafusaku 18kg, vitamin C 13kg, shaddock Skin extract solution 25kg, Chinese medicine extract 25kg, trace element 10kg, biogas slurry 23kg, dregs of beans 4kg, stalk 5kg, animal human bones 2kg。
Described growth stimulator, its preparation method is comprised the following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.7 DEG C/min's Temperature is risen to 53 DEG C by speed, and relative humidity is 28%, after fermentation 13h, cools the temperature to 28 DEG C, and relative humidity is 50%, hair Ferment 7h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, water bath with thermostatic control stirring 1.1h, filtering, take at 33 DEG C Filtrate, puts into watering can.
Described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water, in cotton-shaped, is filtered to white flesh, The 1/3 of filtrate content is concentrated into, the immersion 4.5h that concentration is 75% ethanol is added, ethanol is reclaimed, ethyl acetate is added, heated back Stream more than 2 times, each 23min reclaims ethyl acetate, you can.
Described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 18kg, akebi 2.5kg, henon bamboo Leaf 23kg, radix stellariae dichotomae 8kg, caulis lonicerae 15kg, Caulis Perillae 17kg, root bark of Chinese wolf-berry 20kg, Chinese violet 20kg.
Described medicament, is made up of following raw material by weight:50% captan 1.8kg, 75% Bravo 2.5kg, S-Ethyl ethylthio sulfonate 7kg, capsule of weeping forsythia powder 13kg, stone-like omphalia powder 5kg, humic sour soil 25kg, nitrogen-fixing bacteria 1.3kg, bacillus subtilis 2kg。
Described modifying agent, is made up of following raw material by weight:Perlite 15kg, waste mushroom stick 18kg, husk 8kg, river bottom mud 15kg, tealeaf residue 12kg.
Described accelerator, urea 25kg, potassium dihydrogen phosphate 22kg, potassium sulfate 15kg, 3-Hydroxyretinol kg, vitamin C3.5kg, glycine 0.3kg, rhizoma alismatis 10kg, Lygodium japonicum 13kg, rde bean 10kg, gemma agrimoninae 3.5kg, Ramulus Taxilli 1.5kg.
Embodiment 4
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:The purple perilla seed for choose full grains, having no mechanical damage, is first soaked with pretreatment fluid 30min, seed is pulled out and is drained, then purple perilla seed is placed in into 30 DEG C, and relative humidity is in the environment of 50%, seed to be tiled, Covered with towel, by the standard of 500m1/m2, a growth stimulator was sprayed every 2 hours, after 2d, seed taken out, with medicament pair Seed is dressed seed, and wherein seed and medicament compare mixing for standby use by 1: 1.8 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 150kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 100kg, until seed sprouting, this phase Between keep nursery bed temperature be 26.5 DEG C, relative humidity is 35%, whne right seedling it is long to 8cm when, to seedbed spray accelerator, by temperature 28.5 DEG C are risen to, relative humidity is 22%, period carries out artificial weeding to seedbed, treat that seedling is long and transplanted to by more than 20cm.
Described pretreatment fluid, is by 1.5 by 0.5% hydrogen peroxide, 12% alcohol and 0.25% physiological saline: 0.8: 3 mass ratio mixing, the temperature of ultrasonically treated 3min, wherein pretreatment fluid is 27 DEG C.
Described growth stimulator, is made up of following raw material by weight:Nafusaku 15kg, vitamin C 11kg, shaddock Skin extract solution 30kg, Chinese medicine extract 30kg, trace element 8kg, biogas slurry 20kg, dregs of beans 5kg, stalk 4kg, animal human bones 2.5kg。
Described growth stimulator, its preparation method is comprised the following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.5 DEG C/min's Temperature is risen to 50 DEG C by speed, and relative humidity is 30%, after fermentation 12h, cools the temperature to 25 DEG C, and relative humidity is 55%, hair Ferment 8h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, water bath with thermostatic control stirring 1h, filtering, take filter at 30 DEG C Liquid, puts into watering can.
Described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water, in cotton-shaped, is filtered to white flesh, The 1/3 of filtrate content is concentrated into, the immersion 5h that concentration is 75% ethanol is added, ethanol is reclaimed, ethyl acetate is added, is heated to reflux More than 2 times, each 20min reclaims ethyl acetate, you can.
Described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 22kg, akebi 3kg, lophatherum gracile 25kg, radix stellariae dichotomae 6kg, caulis lonicerae 12kg, Caulis Perillae 15kg, root bark of Chinese wolf-berry 22kg, Chinese violet 18kg.
Described medicament, is made up of following raw material by weight:50% captan 2kg, 75% Bravo 2kg, second Allicin 5kg, capsule of weeping forsythia powder 10kg, stone-like omphalia powder 7kg, humic sour soil 20kg, nitrogen-fixing bacteria 1.2kg, bacillus subtilis 2.2kg.
Described modifying agent, is made up of following raw material by weight:Perlite 10kg, waste mushroom stick 15kg, husk 10kg, river bottom mud 18kg, tealeaf residue 8kg.
Described accelerator, urea 20kg, potassium dihydrogen phosphate 18kg, potassium sulfate 20kg, 3-Hydroxyretinol .5kg, vitamin C3kg, glycine 0.25kg, rhizoma alismatis 12kg, Lygodium japonicum 15kg, rde bean 8kg, gemma agrimoninae 3kg, Ramulus Taxilli 1kg.
Embodiment 5
A kind of method for culturing seedlings of purple perilla, comprises the following steps:
(1) Seeds preprocess:The purple perilla seed for choose full grains, having no mechanical damage, is first soaked with pretreatment fluid 30min, seed is pulled out and is drained, then purple perilla seed is placed in into 40 DEG C, and relative humidity is in the environment of 50%, seed to be tiled, Covered with towel, by the standard of 550m1/m2, a growth stimulator was sprayed every 2 hours, after 2d, seed taken out, with medicament pair Seed is dressed seed, and wherein seed and medicament compare mixing for standby use by 1: 2 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humic The soil of soil does seedbed, and modifying agent is added in seedbed by 120kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 100kg, until seed sprouting, this phase Between keep nursery bed temperature be 26.5 DEG C, relative humidity is 35%, whne right seedling it is long to 5cm when, to seedbed spray accelerator, by temperature 27.5 DEG C are risen to, relative humidity is 20%, period carries out artificial weeding to seedbed, treat that seedling is long and transplanted to by more than 20cm.
Described pretreatment fluid, is by 1: 1 by 0.5% hydrogen peroxide, 15% alcohol and 0.28% physiological saline: 2.5 mass ratio mixing, the temperature of ultrasonically treated 3min, wherein pretreatment fluid is 25 DEG C.
Described growth stimulator, is made up of following raw material by weight:Nafusaku 15kg, vitamin C 11kg, shaddock Skin extract solution 20kg, Chinese medicine extract 30kg, trace element 12kg, biogas slurry 25kg, dregs of beans 4kg, stalk 5kg, animal human bones 2kg。
Described growth stimulator, its preparation method is comprised the following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.5 DEG C/min's Temperature is risen to 50 DEG C by speed, and relative humidity is 30%, after fermentation 15h, cools the temperature to 25 DEG C, and relative humidity is 55%, hair Ferment 8h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, water bath with thermostatic control stirring 1h, filtering, take filter at 30 DEG C Liquid, puts into watering can.
Described pomelo peel extract solution, the white flesh part of pomelo peel is taken out, and the infusion that adds water, in cotton-shaped, is filtered to white flesh, The 1/3 of filtrate content is concentrated into, the immersion 4h that concentration is 75% ethanol is added, ethanol is reclaimed, ethyl acetate is added, is heated to reflux More than 2 times, each 20min reclaims ethyl acetate, you can.
Described Chinese medicine extract, is made up of following raw material by weight:Sculellaria barbata 15kg, akebi 2kg, lophatherum gracile 23kg, radix stellariae dichotomae 8kg, caulis lonicerae 18kg, Caulis Perillae 17kg, root bark of Chinese wolf-berry 20kg, Chinese violet 18kg.
Described medicament, is made up of following raw material by weight:50% captan 1.5kg, 75% Bravo 2kg, S-Ethyl ethylthio sulfonate 8kg, capsule of weeping forsythia powder 15kg, stone-like omphalia powder 7kg, humic sour soil 20kg, nitrogen-fixing bacteria 1.2kg, bacillus subtilis 1.8kg.
Described modifying agent, is made up of following raw material by weight:Perlite 20kg, waste mushroom stick 18kg, husk 10kg, river bottom mud 12kg, tealeaf residue 13kg.
Described accelerator, urea 30kg, potassium dihydrogen phosphate 25kg, potassium sulfate 15kg, dehydroretinol .5kg, vitamin C3kg, glycine 0.35kg, rhizoma alismatis 8kg, Lygodium japonicum 15kg, rde bean 12kg, gemma agrimoninae 3kg, Ramulus Taxilli 1kg.
Experimental example
This researcher is combined to the nursery of purple perilla treatment, and the seedling cultivated the method for culturing seedlings of above-described embodiment 1~5 into The per mu yield of the purple perilla after motility rate and harvesting is counted, using traditional method for culturing seedlings as control, its result such as table 1 below It is shown:
Table 1
Project Survival rate (%) Per mu yield kg
Embodiment 1 90.2 1728.3
Embodiment 2 91.3 1731.5
Embodiment 3 94.6 1745.9
Embodiment 4 93.4 1738.5
Embodiment 5 92.5 1735.1
Control group 85.9 1375.2
Explanation:The survival rate of the purple perilla seedling that the present invention is cultivated, compared to conventional breeding processes, is survived up to more than 90% Rate improve 5~10.1%, the present invention cultivate purple perilla harvesting after per mu yield in 1728.3~1745.9kg, relative to tradition side The per mu yield of the purple perilla of method plantation, is capable of achieving volume increase 25.6~26.9%, and the mu of purple perilla is effectively improved using this implantation methods Yield.

Claims (9)

1. a kind of method for culturing seedlings of purple perilla, it is characterised in that comprise the following steps:
(1) Seeds preprocess:Full grains, the purple perilla seed that has no mechanical damage are chosen, first 30 are soaked with pretreatment fluid~ 35min, seed is pulled out and is drained, then purple perilla seed is placed in into 30~40 DEG C, and relative humidity is that in the environment of 50~55%, will plant Son tiling, is covered with towel, by the standard of 500~550ml/m2, a growth stimulator is sprayed every 2 hours, after 2d, seed is taken Go out, seed is dressed seed with medicament, wherein seed and medicament compare mixing for standby use by 1: 1.8~2.2 quality;
(2) seedbed is built:Select the place of hidding and sunny side, topsoil is deep, fertile loose, draining, it is ventilative good, rich in humous Soil does seedbed, and modifying agent is added in seedbed by 120~150kg/ mus of standard, mixes thoroughly, after placing 7d naturally, can start Sowing;
(3) field management:After planting seedbed is watered, mu irrigation amount is 80~100kg, until seed sprouting, this phase Between keep nursery bed temperature be 24.5~26.5 DEG C, relative humidity be 25~35%, whne right seedling it is long to 5~8cm when, to seedbed spray Accelerator, 27.5~28.5 DEG C are risen to by temperature, and relative humidity is 20~22%, and period carries out artificial weeding to seedbed, treats children Seedling it is long to more than 20cm can transplant.
2. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described pretreatment fluid, be by 0.5~ 0.7% hydrogen peroxide, 12~15% alcohol and 0.25~0.28% physiological saline are by 1~1.5: 0.8~1.2: 2~3 Mass ratio mixes, and the temperature of ultrasonically treated 3~5min, wherein pretreatment fluid is 25~27 DEG C.
3. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described growth stimulator, by it is following by weight The raw material composition of meter:Nafusaku 15~20, vitamin C 11~15, pomelo peel extract solution 20~30, Chinese medicine extract 20~ 30th, trace element 8~12, biogas slurry 20~25, dregs of beans 3~5, stalk 4~6, animal human bones 1.2~2.5.
4. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described growth stimulator, its preparation method, including Following steps:
(1) it is after crushing dregs of beans, stalk and animal human bones, to mix with biogas slurry, fermenting cellar is placed in, with 0.5~0.8 DEG C/min's Temperature is risen to 50~55 DEG C by speed, and relative humidity is 25~30%, after 12~15h of fermentation, cools the temperature to 25~30 DEG C, phase It is 45~55% to humidity, ferment 6~8h, filters to get filtrate;
(2) filtrate that remaining raw material is obtained with step (1) is mixed, 1~1.2h, mistake are stirred in water bath with thermostatic control at 30~35 DEG C Filter, takes filtrate, puts into watering can.
5. the method for culturing seedlings of purple perilla as claimed in claim 3, it is characterised in that described pomelo peel extract solution, by pomelo peel White flesh part take out, to white flesh in cotton-shaped, filtering is concentrated into the 1/3 of filtrate content, and additions concentration is 75% second for the infusion that adds water 4~the 5h of immersion of alcohol, reclaims ethanol, adds ethyl acetate, is heated to reflux more than 2 times, and 20~25min, reclaims acetic acid second every time Ester, you can.
6. the method for culturing seedlings of purple perilla as claimed in claim 3, it is characterised in that described Chinese medicine extract, by following by weight The raw material composition of amount part meter:Sculellaria barbata 15~22, akebi 2~3, lophatherum gracile 20~25, radix stellariae dichotomae 6~10, caulis lonicerae 12~18, Caulis Perillae 15~19, the root bark of Chinese wolf-berry 18~22, Chinese violet 18~22.
7. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described medicament, by it is following by weight Raw material composition:50% captan 1.5~2,75% Bravo 2~3, S-Ethyl ethylthio sulfonate 5~8, capsule of weeping forsythia powder 10~15, stone-like omphalia powder 4~ 7th, humic sour soil 20~30, nitrogen-fixing bacteria 1.2~1.5, bacillus subtilis 1.8~2.2.
8. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described modifying agent, by it is following by weight The raw material composition of meter:Perlite 10~20, waste mushroom stick 15~20, husk 6~10, river bottom mud 12~18, tealeaf residue 8~15.
9. the method for culturing seedlings of purple perilla as claimed in claim 1, it is characterised in that described accelerator, urea 20~30, phosphoric acid Potassium dihydrogen 18~25, potassium sulfate 10~20, dehydroretinol .5~3.5, vitamin C 3~4, glycine 0.25~0.35, rhizoma alismatis 8 ~12, Lygodium japonicum 10~15, rde bean 8~12, gemma agrimoninae 3~4, Ramulus Taxilli 1~2.
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CN107517693A (en) * 2017-08-24 2017-12-29 江苏奥沃思特生物科技有限公司 A kind of purple-perilla cultivation method
CN107960283A (en) * 2017-11-23 2018-04-27 桂林国农生态农业有限公司 A kind of breeding method of dragon ridge capsicum
CN108401803A (en) * 2018-03-22 2018-08-17 佛山推启农业研究院(普通合伙) A kind of Gorgon fruit implantation methods rich in various trace elements
CN109122063A (en) * 2018-06-29 2019-01-04 平凉锐博知识产权服务有限公司 A kind of organic implantation methods of purple perilla
CN109220596A (en) * 2018-08-28 2019-01-18 重庆市药物种植研究所 A kind of method for culturing seedlings of purple perilla breeding
CN109463238A (en) * 2018-12-19 2019-03-15 湖南新汇制药股份有限公司 A kind of breeding method of rhizoma polygonati stem tuber

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107517693A (en) * 2017-08-24 2017-12-29 江苏奥沃思特生物科技有限公司 A kind of purple-perilla cultivation method
CN107960283A (en) * 2017-11-23 2018-04-27 桂林国农生态农业有限公司 A kind of breeding method of dragon ridge capsicum
CN108401803A (en) * 2018-03-22 2018-08-17 佛山推启农业研究院(普通合伙) A kind of Gorgon fruit implantation methods rich in various trace elements
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CN109220596A (en) * 2018-08-28 2019-01-18 重庆市药物种植研究所 A kind of method for culturing seedlings of purple perilla breeding
CN109463238A (en) * 2018-12-19 2019-03-15 湖南新汇制药股份有限公司 A kind of breeding method of rhizoma polygonati stem tuber

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Application publication date: 20170609