CN106715720B - 用于在核酸测定中改善解链分辨和多重性的探针 - Google Patents

用于在核酸测定中改善解链分辨和多重性的探针 Download PDF

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CN106715720B
CN106715720B CN201580050926.4A CN201580050926A CN106715720B CN 106715720 B CN106715720 B CN 106715720B CN 201580050926 A CN201580050926 A CN 201580050926A CN 106715720 B CN106715720 B CN 106715720B
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probe
sequence
nucleic acid
target nucleic
cleavable
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CN106715720A (zh
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斯科特·C·约翰逊
尼古拉斯·阿拉贝
道格·惠特曼
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Luminex Corp
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1201568A1 (en) * 2011-09-29 2015-09-04 露美内克丝公司 Hydrolysis probes
US9657332B2 (en) * 2013-08-09 2017-05-23 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2016025452A1 (en) * 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
EP3234814A4 (en) * 2014-12-15 2018-09-19 Luminex Corporation Detailed assay protocol specification
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
JP7063886B2 (ja) * 2016-09-15 2022-05-09 エフ．ホフマン－ラ ロシュ アーゲー マルチプレックスリアルタイムｐｃｒを実施する方法
JP6754301B2 (ja) * 2017-01-05 2020-09-09 株式会社日立製作所 ドロップレットデジタルｐｃｒの測定方法および測定装置
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
WO2019028361A1 (en) * 2017-08-04 2019-02-07 Stratos Genomics Inc. SINGLE MOLECULE NUCLEIC ACID DETECTION BY CLEARING MESAPPARITIONS
KR102237237B1 (ko) * 2017-11-28 2021-04-07 에스에프씨 주식회사 소광자 및 이의 용도
CA3088250A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
KR102098710B1 (ko) * 2018-01-31 2020-04-08 한국과학기술원 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법
WO2019178337A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Multiplex amplification of nucleic acids employing lowering of denaturation temperature and increasing the annealing temperature
CN108504655B (zh) * 2018-03-27 2020-10-20 浙江大学 一种用于制备DNA Ladder的引物对、DNA片段及方法
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
CN113227394A (zh) 2018-12-28 2021-08-06 卢米耐克斯公司 用于检测变体核苷酸的方法
US20220213540A1 (en) * 2019-04-22 2022-07-07 POSTECH Research and Business Development Foundation Novel probe set for isothermal one-pot reaction, and uses thereof
KR102336454B1 (ko) * 2019-06-17 2021-12-08 강릉원주대학교산학협력단 핵산 검출 방법
CN112921117B (zh) * 2019-12-06 2022-10-14 杭州百殷生物科技有限公司 一种用于检测多种人乳头状瘤病毒分型的试剂盒及操作方法
WO2021175762A1 (en) * 2020-03-03 2021-09-10 Universiteit Gent Hydrolysis-based probe and method for str genotyping
CN112329936B (zh) * 2020-11-05 2023-03-24 西北工业大学 一种基于多种群遗传算法和相似性计算的信号延拓方法
WO2022132955A2 (en) * 2020-12-16 2022-06-23 Proof Diagnostics, Inc. Coronavirus rapid diagnostics
CN113073152A (zh) * 2021-05-12 2021-07-06 广州普世利华科技有限公司 一种用于检测乙型流感病毒的lamp引物、探针、试剂盒
CN113201593B (zh) * 2021-05-25 2024-01-23 广州盛世中豪运动科技有限公司 用于检测actn3基因型的引物探针组合产品
US20250146060A1 (en) * 2021-09-16 2025-05-08 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences
WO2023108014A1 (en) 2021-12-07 2023-06-15 Luminex Corporation Methods and compositions for nucleic acid analysis
EP4547758A1 (en) * 2022-06-29 2025-05-07 Life Technologies Corporation Multiplex dye compounds
WO2024101758A1 (ko) * 2022-11-09 2024-05-16 고려대학교 산학협력단 연장 매개 자가 접힘을 이용한 새로운 핵산 등온 증폭 방법
CN115896250A (zh) * 2023-02-23 2023-04-04 武汉大学 用于检测目标rna或单链dna的组合物及其应用
WO2025053620A1 (ko) * 2023-09-08 2025-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
CN118879840B (zh) * 2024-08-19 2025-10-21 福州共荧生物科技有限公司 一种基于pcr的多色荧光通道检测方法及应用

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5965364A (en) 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69333650T2 (de) 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6913881B1 (en) * 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
WO1998059233A1 (en) 1997-06-23 1998-12-30 Luminex Corporation Interlaced lasers for multiple fluorescence measurement
US6274316B1 (en) 1997-07-03 2001-08-14 Id Biomedical Corporation Compositions and methods for detecting vancomycin resistant enterococci by cycling probe reactions
US20020102571A1 (en) * 1997-07-24 2002-08-01 Jane Theaker Use of 2'-O-methyl RNA as hybridisation probe
JP4357112B2 (ja) 1997-10-14 2009-11-04 ルミネックス コーポレイション 精密蛍光染色された粒子及びその製造及び使用方法
ATE239801T1 (de) 1998-01-22 2003-05-15 Luminex Corp Mikropartikel mit multiplen fluoreszenz-signalen
US6592822B1 (en) 1998-05-14 2003-07-15 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
AU4184099A (en) 1998-05-14 1999-11-29 Luminex Corporation Zero dead time architecture and method for flow cytometer
AU3996299A (en) 1998-05-14 1999-11-29 Luminex Corporation Diode laser based measurement apparatus
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
US6951722B2 (en) 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
AU6779200A (en) 1999-08-17 2001-03-13 Luminex Corporation Encapsulation of fluorescent particles
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
EP1358352B1 (en) 2000-10-14 2009-02-11 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US7226737B2 (en) 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
AU2002229435B2 (en) 2001-01-25 2007-06-07 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
EP1594950A4 (en) 2003-01-29 2007-07-25 454 Corp PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
CN101238221B (zh) * 2005-04-12 2011-11-16 Novia公司名下的现场Rcp公司 新型环形探针及其在鉴定生物分子中的用途
JP2008539761A (ja) * 2005-05-11 2008-11-20 ストラタジーン カリフォルニア スナップバックオリゴヌクレオチドプローブ
CN101268199A (zh) * 2005-07-15 2008-09-17 阿普尔拉股份有限公司 核酸扩增的检测
JP4898210B2 (ja) * 2005-08-09 2012-03-14 株式会社ビー・エム・エル サポウイルスの検出方法
US20090068643A1 (en) 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US20120122095A1 (en) * 2006-01-12 2012-05-17 Eragen Biosciences, Inc. Materials and methods for the detection of anthrax related toxin genes
WO2007127935A1 (en) * 2006-04-27 2007-11-08 J. David Gladstone Institutes Dephosphorylation of hdac7 by myosin phosphatase
US8296088B2 (en) 2006-06-02 2012-10-23 Luminex Corporation Systems and methods for performing measurements of one or more materials
US7910302B2 (en) * 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
WO2008089286A1 (en) 2007-01-17 2008-07-24 Meridian Bioscience Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)
CN101918587B (zh) * 2007-03-08 2013-03-27 拜欧法诊断有限公司 用于熔解分析的引物
JP2009050217A (ja) * 2007-08-28 2009-03-12 Olympus Corp 標的ｄｎａを検出する方法
WO2009074882A2 (en) 2007-11-02 2009-06-18 Luminex Molecular Diagnostics, Inc. One-step target detection assay
WO2009135093A2 (en) 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
US10227641B2 (en) * 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
WO2010036359A2 (en) 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
US8440406B2 (en) 2009-10-23 2013-05-14 Luminex Corporation Amplification primers with non-standard bases for increased reaction specificity
WO2011063388A2 (en) * 2009-11-23 2011-05-26 Becton, Dickinson And Company Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
GB201004292D0 (en) * 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
US8859266B2 (en) * 2010-04-28 2014-10-14 University Of Central Florida Research Foundation, Inc. Binary probe system for sensitive detection of target analytes
CN101871007B (zh) * 2010-05-07 2014-04-30 无锡锐奇基因生物科技有限公司 用标记探针进行检测和熔解曲线分析的方法
EP2569447A4 (en) * 2010-05-14 2013-11-27 Fluidigm Corp ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY
WO2012024639A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Polymerase assay with a fret substrate
US20120052492A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria spp. and use thereof
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2012150749A1 (en) * 2011-05-04 2012-11-08 Seegene, Inc. Detection of target nucleic acid sequences by po cleavage and hybridization
WO2012151473A2 (en) 2011-05-04 2012-11-08 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
WO2013166466A1 (en) * 2012-05-04 2013-11-07 Bio-Rad Laboratories, Inc. Hot-start digital pcr
US9617589B2 (en) * 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
US9657332B2 (en) 2013-08-09 2017-05-23 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2016025452A1 (en) * 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays

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