JP6664381B2 - 核酸アッセイにおける改善された融解識別と多重化のためのプローブ - Google Patents

核酸アッセイにおける改善された融解識別と多重化のためのプローブ Download PDF

Info

Publication number
JP6664381B2
JP6664381B2 JP2017507861A JP2017507861A JP6664381B2 JP 6664381 B2 JP6664381 B2 JP 6664381B2 JP 2017507861 A JP2017507861 A JP 2017507861A JP 2017507861 A JP2017507861 A JP 2017507861A JP 6664381 B2 JP6664381 B2 JP 6664381B2
Authority
JP
Japan
Prior art keywords
probe
sequence
nucleic acid
cleavable
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017507861A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017523799A (ja
JP2017523799A5 (enExample
Inventor
スコット シー． ジョンソン
スコット シー． ジョンソン
ニコラス アラブ
ニコラス アラブ
ダグ ホイットマン
ダグ ホイットマン
Original Assignee
ルミネックス コーポレーション
ルミネックス コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ルミネックス コーポレーション, ルミネックス コーポレーション filed Critical ルミネックス コーポレーション
Publication of JP2017523799A publication Critical patent/JP2017523799A/ja
Publication of JP2017523799A5 publication Critical patent/JP2017523799A5/ja
Priority to JP2020024843A priority Critical patent/JP6929398B2/ja
Application granted granted Critical
Publication of JP6664381B2 publication Critical patent/JP6664381B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2017507861A 2014-08-11 2015-08-11 核酸アッセイにおける改善された融解識別と多重化のためのプローブ Active JP6664381B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2020024843A JP6929398B2 (ja) 2014-08-11 2020-02-18 核酸アッセイにおける改善された融解識別と多重化のためのプローブ

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462035783P 2014-08-11 2014-08-11
US62/035,783 2014-08-11
PCT/US2015/044609 WO2016025452A1 (en) 2014-08-11 2015-08-11 Probes for improved melt discrimination and multiplexing in nucleic acids assays

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020024843A Division JP6929398B2 (ja) 2014-08-11 2020-02-18 核酸アッセイにおける改善された融解識別と多重化のためのプローブ

Publications (3)

Publication Number Publication Date
JP2017523799A JP2017523799A (ja) 2017-08-24
JP2017523799A5 JP2017523799A5 (enExample) 2018-08-23
JP6664381B2 true JP6664381B2 (ja) 2020-03-13

Family

ID=55266966

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017507861A Active JP6664381B2 (ja) 2014-08-11 2015-08-11 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
JP2020024843A Active JP6929398B2 (ja) 2014-08-11 2020-02-18 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
JP2021130466A Active JP7231680B2 (ja) 2014-08-11 2021-08-10 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
JP2023022049A Pending JP2023053361A (ja) 2014-08-11 2023-02-16 核酸アッセイにおける改善された融解識別と多重化のためのプローブ

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020024843A Active JP6929398B2 (ja) 2014-08-11 2020-02-18 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
JP2021130466A Active JP7231680B2 (ja) 2014-08-11 2021-08-10 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
JP2023022049A Pending JP2023053361A (ja) 2014-08-11 2023-02-16 核酸アッセイにおける改善された融解識別と多重化のためのプローブ

Country Status (7)

Country Link
US (5) US9982291B2 (enExample)
EP (5) EP4613872A3 (enExample)
JP (4) JP6664381B2 (enExample)
KR (3) KR20220155394A (enExample)
CN (2) CN106715720B (enExample)
CA (1) CA2956812C (enExample)
WO (1) WO2016025452A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2761032B1 (en) * 2011-09-29 2017-07-19 Luminex Corporation Hydrolysis probes
EP3030680B1 (en) 2013-08-09 2018-10-03 Luminex Corporation Melt discrimination and multiplexing in nucleic acid assays
EP4613872A3 (en) 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2016100046A1 (en) * 2014-12-15 2016-06-23 Luminex Corporation Detailed assay protocol specification
WO2017214561A1 (en) * 2016-06-10 2017-12-14 Life Technologies Corporation Methods and compositions for nucleic acid amplification
EP3512959B1 (en) * 2016-09-15 2021-08-11 Roche Diagnostics GmbH Methods for performing multiplexed pcr
JP6754301B2 (ja) * 2017-01-05 2020-09-09 株式会社日立製作所 ドロップレットデジタルｐｃｒの測定方法および測定装置
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
WO2019028361A1 (en) * 2017-08-04 2019-02-07 Stratos Genomics Inc. SINGLE MOLECULE NUCLEIC ACID DETECTION BY CLEARING MESAPPARITIONS
KR102237237B1 (ko) * 2017-11-28 2021-04-07 에스에프씨 주식회사 소광자 및 이의 용도
WO2019144107A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
KR102098710B1 (ko) * 2018-01-31 2020-04-08 한국과학기술원 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법
US20190284609A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Fast, multiplex amplification of nucleic acids
CN108504655B (zh) * 2018-03-27 2020-10-20 浙江大学 一种用于制备DNA Ladder的引物对、DNA片段及方法
MX2021001385A (es) 2018-08-09 2021-06-23 Speedx Pty Ltd Detección múltiplex de ácidos nucleicos.
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
US20200208207A1 (en) 2018-12-28 2020-07-02 Luminex Corporation Methods for detecting variant nucleotides
EP3943614B1 (en) * 2019-04-22 2024-02-21 POSTECH Research and Business Development Foundation Novel probe set for isothermal one-pot reaction, and uses thereof
WO2020256378A1 (ko) * 2019-06-17 2020-12-24 강릉원주대학교산학협력단 핵산 검출 방법
CN112921117B (zh) * 2019-12-06 2022-10-14 杭州百殷生物科技有限公司 一种用于检测多种人乳头状瘤病毒分型的试剂盒及操作方法
CN115943218B (zh) * 2020-03-03 2025-05-09 根特大学 基于水解的探针和str基因分型的方法
CN112329936B (zh) * 2020-11-05 2023-03-24 西北工业大学 一种基于多种群遗传算法和相似性计算的信号延拓方法
US20240327933A1 (en) * 2020-12-16 2024-10-03 The Broad Institute, Inc. Coronavirus rapid diagnostics
CN113073152A (zh) * 2021-05-12 2021-07-06 广州普世利华科技有限公司 一种用于检测乙型流感病毒的lamp引物、探针、试剂盒
CN113201593B (zh) * 2021-05-25 2024-01-23 广州盛世中豪运动科技有限公司 用于检测actn3基因型的引物探针组合产品
AU2022346850A1 (en) * 2021-09-16 2024-03-28 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences
US20230348957A1 (en) 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
EP4547758A1 (en) * 2022-06-29 2025-05-07 Life Technologies Corporation Multiplex dye compounds
WO2024101758A1 (ko) * 2022-11-09 2024-05-16 고려대학교 산학협력단 연장 매개 자가 접힘을 이용한 새로운 핵산 등온 증폭 방법
CN115896250A (zh) * 2023-02-23 2023-04-04 武汉大学 用于检测目标rna或单链dna的组合物及其应用
WO2025053620A1 (ko) * 2023-09-08 2025-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
CN118879840B (zh) * 2024-08-19 2025-10-21 福州共荧生物科技有限公司 一种基于pcr的多色荧光通道检测方法及应用

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5965364A (en) 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6913881B1 (en) * 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
DK1179600T3 (da) 1996-06-04 2005-09-05 Univ Utah Res Found Overvågning af hybridisering under PCR
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
WO1998059233A1 (en) 1997-06-23 1998-12-30 Luminex Corporation Interlaced lasers for multiple fluorescence measurement
US6274316B1 (en) * 1997-07-03 2001-08-14 Id Biomedical Corporation Compositions and methods for detecting vancomycin resistant enterococci by cycling probe reactions
US20020102571A1 (en) * 1997-07-24 2002-08-01 Jane Theaker Use of 2'-O-methyl RNA as hybridisation probe
WO1999019515A1 (en) 1997-10-14 1999-04-22 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
KR100593712B1 (ko) 1998-01-22 2006-06-30 루미넥스 코포레이션 다수의 형광 시그널을 갖는 마이크로입자
CA2331897C (en) 1998-05-14 2008-11-18 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
JP4471494B2 (ja) 1998-05-14 2010-06-02 ルミネックス コーポレイション ダイオードレーザをベースとした測定装置
WO1999057955A1 (en) 1998-05-14 1999-11-18 Luminex Corporation Zero dead time architecture and method for flow cytometer
WO2000028082A1 (fr) 1998-11-09 2000-05-18 Eiken Kagaku Kabushiki Kaisha Procede de synthese d'acide nucleique
US6951722B2 (en) 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
ATE324588T1 (de) 1999-08-17 2006-05-15 Luminex Corp Verkapselung von fluoreszierenden partikeln
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
CA2425747C (en) 2000-10-14 2012-01-24 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
WO2002059354A2 (en) 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides for use as tags and tag complements, manufacture and use thereof
US7226737B2 (en) 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP2918595B1 (en) 2003-07-05 2019-12-11 The Johns-Hopkins University Method and compositions for detection and enumeration of genetic variations
US7927797B2 (en) 2004-01-28 2011-04-19 454 Life Sciences Corporation Nucleic acid amplification with continuous flow emulsion
EP1871896B1 (en) 2005-04-12 2015-06-03 Olink AB Circle probes and their use in the identification of biomolecules
US20070020656A1 (en) * 2005-05-11 2007-01-25 Stratagene California Snapback oligonucleotide probe
CN101268199A (zh) * 2005-07-15 2008-09-17 阿普尔拉股份有限公司 核酸扩增的检测
JP4898210B2 (ja) * 2005-08-09 2012-03-14 株式会社ビー・エム・エル サポウイルスの検出方法
US20090068643A1 (en) 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
WO2008048334A2 (en) * 2006-01-12 2008-04-24 Eragen Biosciences, Inc. Materials and methods for the detection of anthrax related toxin genes
US20080200566A1 (en) * 2006-04-27 2008-08-21 The J. David Gladstone Institutes Dephosphorylation of HDAC7 By Myosin Phosphatase
US8296088B2 (en) 2006-06-02 2012-10-23 Luminex Corporation Systems and methods for performing measurements of one or more materials
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007333040B2 (en) 2006-12-13 2013-02-07 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
AU2008206221A1 (en) 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP)
CA2680121C (en) 2007-03-08 2016-05-10 Idaho Technology Primers for melting curve analysis in methods of nucleic acid analysis
JP2009050217A (ja) * 2007-08-28 2009-03-12 Olympus Corp 標的ｄｎａを検出する方法
EP2215245A2 (en) 2007-11-02 2010-08-11 Luminex Molecular Diagnostics, Inc. One-step target detection assay
US10227641B2 (en) * 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP3150727B1 (en) 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
WO2010036359A2 (en) 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
WO2011050278A1 (en) 2009-10-23 2011-04-28 Eragen Biosciences, Inc. Amplification primers with non-standard bases for increased reaction specificity
WO2011063388A2 (en) * 2009-11-23 2011-05-26 Becton, Dickinson And Company Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
US9248422B2 (en) 2010-02-23 2016-02-02 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
GB201004292D0 (en) * 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
US8859266B2 (en) * 2010-04-28 2014-10-14 University Of Central Florida Research Foundation, Inc. Binary probe system for sensitive detection of target analytes
CN101871007B (zh) * 2010-05-07 2014-04-30 无锡锐奇基因生物科技有限公司 用标记探针进行检测和熔解曲线分析的方法
US20140186827A1 (en) 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
US10301674B2 (en) * 2010-08-20 2019-05-28 Life Technologies Corporation Polymerase assay with a FRET substrate
US20120052492A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria spp. and use thereof
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
MX342067B (es) * 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.
US20130310269A1 (en) * 2012-05-04 2013-11-21 Austin So Hot-start digital pcr
CN108587867B (zh) * 2012-05-25 2021-12-17 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
EP3030680B1 (en) 2013-08-09 2018-10-03 Luminex Corporation Melt discrimination and multiplexing in nucleic acid assays
EP4613872A3 (en) * 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays

Also Published As

Publication number Publication date
EP3180449A1 (en) 2017-06-21
EP4299760B1 (en) 2025-07-09
EP4299760A2 (en) 2024-01-03
EP3180449A4 (en) 2018-04-11
JP2017523799A (ja) 2017-08-24
US12480152B2 (en) 2025-11-25
US20160040219A1 (en) 2016-02-11
EP4613872A2 (en) 2025-09-10
US20180245139A1 (en) 2018-08-30
CA2956812C (en) 2023-04-11
US9982291B2 (en) 2018-05-29
CA3190291A1 (en) 2016-02-18
JP2021175411A (ja) 2021-11-04
US20170321259A1 (en) 2017-11-09
CN113637728A (zh) 2021-11-12
JP2023053361A (ja) 2023-04-12
EP3180449B1 (en) 2019-10-09
CA2956812A1 (en) 2016-02-18
JP2020096626A (ja) 2020-06-25
JP6929398B2 (ja) 2021-09-01
WO2016025452A1 (en) 2016-02-18
KR20210048598A (ko) 2021-05-03
US20200362396A1 (en) 2020-11-19
KR102246600B1 (ko) 2021-05-03
US10752939B2 (en) 2020-08-25
KR20220155394A (ko) 2022-11-22
EP3653726B1 (en) 2021-10-27
JP7231680B2 (ja) 2023-03-01
EP3653726A1 (en) 2020-05-20
EP4613872A3 (en) 2025-11-12
EP3967771B1 (en) 2023-10-04
EP4299760A3 (en) 2024-02-21
EP3967771A1 (en) 2022-03-16
CN106715720B (zh) 2021-08-13
KR20170041897A (ko) 2017-04-17
CN106715720A (zh) 2017-05-24
CN113637728B (zh) 2025-04-29
KR102465287B1 (ko) 2022-11-09
US20210189469A1 (en) 2021-06-24
US10975419B2 (en) 2021-04-13

Similar Documents

Publication Publication Date Title
JP7231680B2 (ja) 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
US20220154257A1 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
HK40070845A (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
CA3190291C (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
HK1236579A1 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
HK1236579B (zh) 用於在核酸测定中改善解链分辨和多重性的探针
HK1223404B (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170220

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180711

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180711

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190522

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190530

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190815

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200122

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200218

R150 Certificate of patent or registration of utility model

Ref document number: 6664381

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250