CA2956812C - Probes for improved melt discrimination and multiplexing in nucleic acid assays - Google Patents
Probes for improved melt discrimination and multiplexing in nucleic acid assays Download PDFInfo
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- CA2956812C CA2956812C CA2956812A CA2956812A CA2956812C CA 2956812 C CA2956812 C CA 2956812C CA 2956812 A CA2956812 A CA 2956812A CA 2956812 A CA2956812 A CA 2956812A CA 2956812 C CA2956812 C CA 2956812C
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Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013049613A1 (en) * | 2011-09-29 | 2013-04-04 | Luminex Corporation | Hydrolysis probes |
| EP4321867B1 (en) | 2013-08-09 | 2025-10-01 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| KR20220155394A (ko) * | 2014-08-11 | 2022-11-22 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
| JP6740242B2 (ja) * | 2014-12-15 | 2020-08-12 | ルミネックス コーポレーション | 詳細検定プロトコル仕様 |
| WO2017214561A1 (en) * | 2016-06-10 | 2017-12-14 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| JP7221199B2 (ja) * | 2016-09-15 | 2023-02-20 | エフ． ホフマン－ラ ロシュ エージー． | マルチプレックスｐｃｒの実施方法 |
| JP6754301B2 (ja) * | 2017-01-05 | 2020-09-09 | 株式会社日立製作所 | ドロップレットデジタルｐｃｒの測定方法および測定装置 |
| CN108823287B (zh) | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| WO2019028361A1 (en) * | 2017-08-04 | 2019-02-07 | Stratos Genomics Inc. | SINGLE MOLECULE NUCLEIC ACID DETECTION BY CLEARING MESAPPARITIONS |
| KR102237237B1 (ko) * | 2017-11-28 | 2021-04-07 | 에스에프씨 주식회사 | 소광자 및 이의 용도 |
| AU2019209444A1 (en) * | 2018-01-22 | 2020-07-30 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| KR102098710B1 (ko) * | 2018-01-31 | 2020-04-08 | 한국과학기술원 | 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법 |
| US20190284609A1 (en) | 2018-03-14 | 2019-09-19 | Luminex Corporation | Fast, multiplex amplification of nucleic acids |
| CN108504655B (zh) * | 2018-03-27 | 2020-10-20 | 浙江大学 | 一种用于制备DNA Ladder的引物对、DNA片段及方法 |
| SG11202101140TA (en) * | 2018-08-09 | 2021-03-30 | Speedx Pty Ltd | Multiplex detection of nucleic acids |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| WO2020139671A1 (en) | 2018-12-28 | 2020-07-02 | Luminex Corporation | Methods for detecting variant nucleotides |
| WO2020218831A1 (ko) * | 2019-04-22 | 2020-10-29 | 포항공과대학교 산학협력단 | 신규한 등온 단일 반응용 프로브 세트 및 이의 용도 |
| WO2020256378A1 (ko) * | 2019-06-17 | 2020-12-24 | 강릉원주대학교산학협력단 | 핵산 검출 방법 |
| CN112921117B (zh) * | 2019-12-06 | 2022-10-14 | 杭州百殷生物科技有限公司 | 一种用于检测多种人乳头状瘤病毒分型的试剂盒及操作方法 |
| CN115943218B (zh) * | 2020-03-03 | 2025-05-09 | 根特大学 | 基于水解的探针和str基因分型的方法 |
| CN112329936B (zh) * | 2020-11-05 | 2023-03-24 | 西北工业大学 | 一种基于多种群遗传算法和相似性计算的信号延拓方法 |
| US20240327933A1 (en) * | 2020-12-16 | 2024-10-03 | The Broad Institute, Inc. | Coronavirus rapid diagnostics |
| CN113073152A (zh) * | 2021-05-12 | 2021-07-06 | 广州普世利华科技有限公司 | 一种用于检测乙型流感病毒的lamp引物、探针、试剂盒 |
| CN113201593B (zh) * | 2021-05-25 | 2024-01-23 | 广州盛世中豪运动科技有限公司 | 用于检测actn3基因型的引物探针组合产品 |
| AU2022346850A1 (en) * | 2021-09-16 | 2024-03-28 | Cepheid | Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences |
| WO2023108014A1 (en) | 2021-12-07 | 2023-06-15 | Luminex Corporation | Methods and compositions for nucleic acid analysis |
| EP4547861A1 (en) * | 2022-06-29 | 2025-05-07 | Life Technologies Corporation | Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing |
| WO2024101758A1 (ko) * | 2022-11-09 | 2024-05-16 | 고려대학교 산학협력단 | 연장 매개 자가 접힘을 이용한 새로운 핵산 등온 증폭 방법 |
| CN115896250A (zh) * | 2023-02-23 | 2023-04-04 | 武汉大学 | 用于检测目标rna或单链dna的组合物及其应用 |
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| CN118879840B (zh) * | 2024-08-19 | 2025-10-21 | 福州共荧生物科技有限公司 | 一种基于pcr的多色荧光通道检测方法及应用 |
Family Cites Families (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4284412A (en) | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
| US4498766A (en) | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
| US4661913A (en) | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
| US4767206A (en) | 1984-12-24 | 1988-08-30 | Flow Cytometry Standards Corporation | Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof |
| US4857451A (en) | 1984-12-24 | 1989-08-15 | Flow Cytometry Standards Corporation | Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor |
| US4774189A (en) | 1984-12-24 | 1988-09-27 | Flow Cytometry Standards Corp. | Fluorescent calibration microbeads simulating stained cells |
| US5656493A (en) | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
| US4989977A (en) | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
| US4714682A (en) | 1985-12-11 | 1987-12-22 | Flow Cytometry Standards Corporation | Fluorescent calibration microbeads simulating stained cells |
| US4942124A (en) | 1987-08-11 | 1990-07-17 | President And Fellows Of Harvard College | Multiplex sequencing |
| US5160974A (en) | 1990-06-25 | 1992-11-03 | Flow Science, Inc. | Closed sample cell for use in flow cytometry |
| US6140496A (en) | 1990-10-09 | 2000-10-31 | Benner; Steven Albert | Precursors for deoxyribonucleotides containing non-standard nucleosides |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US6037120A (en) | 1995-10-12 | 2000-03-14 | Benner; Steven Albert | Recognition of oligonucleotides containing non-standard base pairs |
| US5965364A (en) | 1990-10-09 | 1999-10-12 | Benner; Steven Albert | Method for selecting functional deoxyribonucleotide derivatives |
| US5478722A (en) | 1991-02-17 | 1995-12-26 | The Curators Of The University Of Missouri | Preserved cell preparations for flow cytometry and immunology |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| ATE278807T1 (de) | 1992-02-19 | 2004-10-15 | New York Health Res Inst | Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren |
| US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US5981180A (en) | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| EP0852004B1 (en) | 1995-10-11 | 2011-01-19 | Luminex Corporation | Multiplexed analysis of clinical specimens |
| US6913881B1 (en) * | 1996-01-24 | 2005-07-05 | Third Wave Technologies, Inc. | Methods and compositions for detecting target sequences |
| US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
| ATE318327T1 (de) | 1996-06-04 | 2006-03-15 | Univ Utah Res Found | Fluoreszenz-donor-akzeptor paar |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| US6449562B1 (en) | 1996-10-10 | 2002-09-10 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
| US6139800A (en) | 1997-06-23 | 2000-10-31 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| US6274316B1 (en) | 1997-07-03 | 2001-08-14 | Id Biomedical Corporation | Compositions and methods for detecting vancomycin resistant enterococci by cycling probe reactions |
| US20020102571A1 (en) * | 1997-07-24 | 2002-08-01 | Jane Theaker | Use of 2'-O-methyl RNA as hybridisation probe |
| KR20010031140A (ko) | 1997-10-14 | 2001-04-16 | 루미넥스 코포레이션 | 정밀 형광염료 입자 및 그의 제조방법 그리고 그의 사용 |
| AU2464299A (en) | 1998-01-22 | 1999-08-09 | Luminex Corporation | Microparticles with multiple fluorescent signals |
| AU3996299A (en) | 1998-05-14 | 1999-11-29 | Luminex Corporation | Diode laser based measurement apparatus |
| US6592822B1 (en) | 1998-05-14 | 2003-07-15 | Luminex Corporation | Multi-analyte diagnostic system and computer implemented process for same |
| CA2328408C (en) | 1998-05-14 | 2005-02-15 | Luminex Corporation | Zero dead time architecture and method for flow cytometer |
| WO2000028082A1 (fr) | 1998-11-09 | 2000-05-18 | Eiken Kagaku Kabushiki Kaisha | Procede de synthese d'acide nucleique |
| US6951722B2 (en) | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| ATE324588T1 (de) | 1999-08-17 | 2006-05-15 | Luminex Corp | Verkapselung von fluoreszierenden partikeln |
| CN1592792B (zh) | 2000-05-19 | 2010-12-01 | 伊拉根生物科学公司 | 检测核酸的材料和方法 |
| JP4499987B2 (ja) | 2000-10-14 | 2010-07-14 | エラジェン バイオサイエンシズ インコーポレイテッド | 非−標準塩基を使用する固体支持体アッセイ系及び方法 |
| WO2002059355A2 (en) | 2001-01-25 | 2002-08-01 | Tm Bioscience Corporation | Polynucleotides for use as tags and tag complements, manufacture and use thereof |
| US7226737B2 (en) | 2001-01-25 | 2007-06-05 | Luminex Molecular Diagnostics, Inc. | Polynucleotides for use as tags and tag complements, manufacture and use thereof |
| DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| US7927797B2 (en) | 2004-01-28 | 2011-04-19 | 454 Life Sciences Corporation | Nucleic acid amplification with continuous flow emulsion |
| CN101238221B (zh) * | 2005-04-12 | 2011-11-16 | Novia公司名下的现场Rcp公司 | 新型环形探针及其在鉴定生物分子中的用途 |
| AU2006244035A1 (en) * | 2005-05-11 | 2006-11-16 | Stratagene California | Snapback oligonucleotide probe |
| CN101268199A (zh) * | 2005-07-15 | 2008-09-17 | 阿普尔拉股份有限公司 | 核酸扩增的检测 |
| JP4898210B2 (ja) * | 2005-08-09 | 2012-03-14 | 株式会社ビー・エム・エル | サポウイルスの検出方法 |
| US20090068643A1 (en) * | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| US20120122095A1 (en) * | 2006-01-12 | 2012-05-17 | Eragen Biosciences, Inc. | Materials and methods for the detection of anthrax related toxin genes |
| WO2007127935A1 (en) * | 2006-04-27 | 2007-11-08 | J. David Gladstone Institutes | Dephosphorylation of hdac7 by myosin phosphatase |
| US8296088B2 (en) | 2006-06-02 | 2012-10-23 | Luminex Corporation | Systems and methods for performing measurements of one or more materials |
| WO2008070352A2 (en) * | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| KR101433208B1 (ko) | 2006-12-13 | 2014-08-22 | 루미넥스 코포레이션 | 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법 |
| AU2008206221A1 (en) | 2007-01-17 | 2008-07-24 | Meridian Bioscience, Inc. | Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP) |
| CN101918587B (zh) | 2007-03-08 | 2013-03-27 | 拜欧法诊断有限公司 | 用于熔解分析的引物 |
| JP2009050217A (ja) * | 2007-08-28 | 2009-03-12 | Olympus Corp | 標的ｄｎａを検出する方法 |
| WO2009074882A2 (en) | 2007-11-02 | 2009-06-18 | Luminex Molecular Diagnostics, Inc. | One-step target detection assay |
| DK2644707T3 (en) | 2008-04-30 | 2015-06-29 | Integrated Dna Tech Inc | RNase-H-based assays using modified RNA monomers. |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US10227641B2 (en) * | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2010013017A1 (en) | 2008-07-31 | 2010-02-04 | Oxitec Limited | Multiplex amplification and detection |
| GB0909333D0 (en) | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| WO2010036359A2 (en) | 2008-09-25 | 2010-04-01 | Allelogic Biosciences Corporation | Methods of polymerase activity assay |
| EP2491146B1 (en) | 2009-10-23 | 2017-09-20 | Luminex Corporation | Amplification primers with non-standard bases for increased reaction specificity |
| WO2011063388A2 (en) * | 2009-11-23 | 2011-05-26 | Becton, Dickinson And Company | Assay method for target nucleic acid by signal amplification using probe hybridization and restriction |
| GB201004292D0 (en) * | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| US8859266B2 (en) * | 2010-04-28 | 2014-10-14 | University Of Central Florida Research Foundation, Inc. | Binary probe system for sensitive detection of target analytes |
| CN101871007B (zh) * | 2010-05-07 | 2014-04-30 | 无锡锐奇基因生物科技有限公司 | 用标记探针进行检测和熔解曲线分析的方法 |
| SG185543A1 (en) * | 2010-05-14 | 2012-12-28 | Fluidigm Corp | Assays for the detection of genotype, mutations, and/or aneuploidy |
| WO2012024639A1 (en) * | 2010-08-20 | 2012-02-23 | Life Technologies Corporation | Polymerase assay with a fret substrate |
| US20120052492A1 (en) * | 2010-08-30 | 2012-03-01 | Samsung Techwin Co., Ltd. | Oligonucleotides for detecting listeria spp. and use thereof |
| MX340258B (es) | 2011-01-11 | 2016-07-01 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| MX342067B (es) * | 2011-05-04 | 2016-09-09 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda. |
| CN104023834B (zh) | 2011-05-04 | 2016-09-28 | 卢米耐克斯公司 | 用于集成的样品制备、反应和检测的设备与方法 |
| US20130310269A1 (en) * | 2012-05-04 | 2013-11-21 | Austin So | Hot-start digital pcr |
| JP6200948B2 (ja) * | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| EP4321867B1 (en) | 2013-08-09 | 2025-10-01 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
| KR20220155394A (ko) * | 2014-08-11 | 2022-11-22 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
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|
| EEER | Examination request |
Effective date: 20200810 |
|
| EEER | Examination request |
Effective date: 20200810 |
|
| EEER | Examination request |
Effective date: 20200810 |