CN106688988B - Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same - Google Patents
Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same Download PDFInfo
- Publication number
- CN106688988B CN106688988B CN201611207522.4A CN201611207522A CN106688988B CN 106688988 B CN106688988 B CN 106688988B CN 201611207522 A CN201611207522 A CN 201611207522A CN 106688988 B CN106688988 B CN 106688988B
- Authority
- CN
- China
- Prior art keywords
- culture
- poecilobdella manillensis
- water
- culture pond
- manillensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000500851 Poecilobdella manillensis Species 0.000 title claims abstract description 174
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000004321 preservation Methods 0.000 claims abstract description 60
- 238000010438 heat treatment Methods 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 230000007246 mechanism Effects 0.000 claims abstract description 31
- 239000000523 sample Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 178
- 210000004369 blood Anatomy 0.000 claims description 60
- 239000008280 blood Substances 0.000 claims description 60
- 239000000843 powder Substances 0.000 claims description 51
- 239000002689 soil Substances 0.000 claims description 48
- 241000283690 Bos taurus Species 0.000 claims description 37
- 238000002156 mixing Methods 0.000 claims description 28
- 239000011248 coating agent Substances 0.000 claims description 24
- 238000000576 coating method Methods 0.000 claims description 24
- 235000007164 Oryza sativa Nutrition 0.000 claims description 23
- 235000009566 rice Nutrition 0.000 claims description 23
- 238000003466 welding Methods 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 19
- 229910001220 stainless steel Inorganic materials 0.000 claims description 18
- 239000010935 stainless steel Substances 0.000 claims description 18
- 240000000491 Corchorus aestuans Species 0.000 claims description 17
- 235000011777 Corchorus aestuans Nutrition 0.000 claims description 17
- 235000010862 Corchorus capsularis Nutrition 0.000 claims description 17
- 208000007536 Thrombosis Diseases 0.000 claims description 16
- 239000003292 glue Substances 0.000 claims description 16
- 210000002381 plasma Anatomy 0.000 claims description 16
- 239000004576 sand Substances 0.000 claims description 16
- 230000002457 bidirectional effect Effects 0.000 claims description 14
- 238000011049 filling Methods 0.000 claims description 14
- 238000009395 breeding Methods 0.000 claims description 12
- 230000001488 breeding effect Effects 0.000 claims description 12
- 230000002354 daily effect Effects 0.000 claims description 12
- 239000004816 latex Substances 0.000 claims description 10
- 229920000126 latex Polymers 0.000 claims description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 8
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 8
- 239000004677 Nylon Substances 0.000 claims description 8
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 8
- 239000003242 anti bacterial agent Substances 0.000 claims description 8
- 229940088710 antibiotic agent Drugs 0.000 claims description 8
- 239000011425 bamboo Substances 0.000 claims description 8
- 239000003610 charcoal Substances 0.000 claims description 8
- 230000001112 coagulating effect Effects 0.000 claims description 8
- 229920001778 nylon Polymers 0.000 claims description 8
- 241000545744 Hirudinea Species 0.000 claims description 7
- 239000004832 casein glue Substances 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 6
- 210000000436 anus Anatomy 0.000 claims description 5
- 206010061428 decreased appetite Diseases 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 235000019387 fatty acid methyl ester Nutrition 0.000 claims description 5
- 239000004459 forage Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 3
- 230000001706 oxygenating effect Effects 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 3
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000003672 processing method Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 16
- 238000012136 culture method Methods 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 230000000644 propagated effect Effects 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 20
- 230000000052 comparative effect Effects 0.000 description 14
- 241001330002 Bambuseae Species 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 238000006213 oxygenation reaction Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 102000007625 Hirudins Human genes 0.000 description 3
- 108010007267 Hirudins Proteins 0.000 description 3
- 238000007605 air drying Methods 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 3
- 229940006607 hirudin Drugs 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000004584 weight gain Effects 0.000 description 3
- 235000019786 weight gain Nutrition 0.000 description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 241000243818 Annelida Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 241000371027 Poecilobdella Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 101000712605 Theromyzon tessulatum Theromin Proteins 0.000 description 1
- 229940122388 Thrombin inhibitor Drugs 0.000 description 1
- 241000964941 Whitmania Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 235000012255 calcium oxide Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000004530 effect on cardiovascular disease Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000006266 hibernation Effects 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000003868 thrombin inhibitor Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
the invention discloses a Poecilobdella manillensis culture device, which comprises: the device comprises a culture pond and a spawning platform, wherein a heating cable is laid at the bottom of the culture pond, a temperature probe line is arranged below the liquid surface of the culture pond, and the heating cable and the temperature probe line are both connected with a temperature controller; the heat preservation mechanism is erected above the culture pond and the spawning platform, and is provided with a nut rod, a keel, a heat preservation plate and a film shed from bottom to top. The invention also discloses a method for overwintering culture of Poecilobdella manillensis by using the device. According to the invention, through the design of the plurality of culture ponds, the spawning platforms and the heat preservation mechanism in the Poecilobdella manillensis culture equipment, the temperature stability during the overwintering culture period is effectively ensured, and meanwhile, through a scientific culture method, the survival rate of the Poecilobdella manillensis during the overwintering period is effectively improved, the normal growth of the Poecilobdella manillensis is ensured, and the Poecilobdella manillensis is also beneficially propagated.
Description
Technical Field
The invention relates to the field of leech culture, in particular to a Poecilobdella manillensis culture device and a method for overwintering culture of Poecilobdella manillensis by using the same.
Background
poecilobdella manillensis belongs to the genus Poecilobdella in the subclass of Hylobdellidae in the phylum Annelida, commonly called "Whitmania manillensis", and is a precious medical leech. The secretion of saliva gland of Poecilobdella manillensis and the body contain hirudin. Hirudin is a natural specific thrombin inhibitor with strongest effect, is an ideal anticoagulant natural medicament and has better curative effect on cardiovascular diseases, cerebral thrombosis, angina pectoris and the like.
with the deep development of the medicinal value of the poecilobdella manillensis, the market demand potential is huge, the natural fishing is mainly used historically, and the large amount of quicklime is used for improving soil and applying chemical fertilizers and pesticides in agricultural production, so that the industrial waste is discharged to seriously pollute the water body, the living space of the poecilobdella manillensis is smaller and smaller, the wild resources are fewer and smaller, and the contradiction between supply and demand is prominent. In order to obtain hirudin, artificial breeding of poecilobdella manillensis is imperative, so that a plurality of breeding poecilobdella manillensis are emerging all over the world, and a certain breeding scale is formed.
Poecilobdella manillensis is a cold blood temperature-variable animal and has hibernation habit under natural environment conditions. Most Poecilobdella manillensis lives in water, and is usually dormant in soil in winter. However, in an indoor artificial breeding environment, temperature fluctuation is large during breeding due to unstable temperature control of breeding equipment, and in addition, the feeding density and daily management during overwintering breeding have serious influence on the overwintering survival and next-year growth and reproduction of poecilobdella manillensis, even all the poecilobdella manillensis die during the overwintering period, which is a serious economic loss for farmers.
disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
the invention also aims to provide the Poecilobdella manillensis culture equipment and the method for culturing Poecilobdella manillensis through the Poecilobdella manillensis culture equipment, wherein the temperature stability during the overwintering culture period is effectively ensured through the design of a plurality of culture ponds, a spawning platform and a heat preservation mechanism in the Poecilobdella manillensis culture equipment, and meanwhile, the survival rate of the Poecilobdella manillensis during the overwintering period is effectively improved through a scientific culture method, the normal growth of the Poecilobdella manillensis is ensured, and meanwhile, the Poecilobdella manillensis is beneficial.
To achieve these objects and other advantages in accordance with the present invention, there is provided a Poecilobdella manillensis cultivation apparatus including:
the device comprises a plurality of culture ponds and a plurality of spawning platforms which are alternately arranged, wherein heating cables are uniformly laid at the bottoms of the culture ponds, a temperature probe line is arranged below the liquid surface of each culture pond, the heating cables and the temperature probe line are both connected with a temperature controller, a water inlet pipe is arranged above the liquid surface of each culture pond, a water outlet pipe is arranged below the liquid surface, an anti-escape net is arranged at the pipe orifice of each water outlet pipe, and soil is filled in each spawning platform;
the heat preservation mechanism, it erects in breeding the pond and spawning platform top, and from the bottom up is provided with nut pole, fossil fragments, heated board and film canopy, the nut pole sets up in breeding the pond and spawning platform periphery along vertical direction with the form of a plurality of rows and columns, fossil fragments are including the main joist and the false keel that are located the mutually perpendicular and joint of same horizontal plane, the heated board erects in the fossil fragments top, film canopy top-down will the heated board nut pole covers.
Preferably, heating cable has bound the stainless steel electric welding net through the nylon ribbon, the broken tile piece has been placed to stainless steel electric welding net below, the temperature controller is installed in the ammeter case and is connected with the air switch in the ammeter case, air switch passes through copper wire connection with two-way plug-in strip, two-way plug-in strip is connected with power supply line and reserve power supply line.
preferably, the soil filled in the spawning platform is rice field soil, coarse sand or gravel with the thickness of 5-10 cm, and gunny bags are laid on the rice field soil, the coarse sand or the gravel.
preferably, the bottom of the culture pond is also paved with an inflation tube, the air inlet end of the inflation tube is communicated with the air outlet of an air heater, the air inlet of the air heater is connected with an air compressor, the air outlet end of the inflation tube is communicated with a plurality of hollow cylinders, the tops of the hollow cylinders are provided with a plurality of air outlet holes, a whole piece of microporous sponge is pasted above the plurality of air outlet holes and covers the tops of the hollow cylinders, and the microporous sponge is immersed below the liquid level.
Preferably, the side wall of the culture pond close to the spawning platform is arranged in a zigzag ladder shape, a sun shield is fixedly erected above the side wall, the sun shield is parallel to the side wall and shields the side wall to form a space for resting Poecilobdella manillensis, and the side wall of the culture pond is coated with shell powder coating.
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
S1, preparation before cultivation: putting 3-8 cm of water into a culture pond, adjusting the water temperature of the culture pond through a temperature controller and a heating cable, controlling the water temperature to be 23-30 ℃, building a heat preservation mechanism, filling soil into a spawning platform, and paving jute bags on the surface of the soil;
s2, putting poecilobdella manillensis: putting 40-80 Poecilobdella manillensis per square meter into a culture pond for overwintering period culture;
S3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as feed, lifting the film shed to feed Poecilobdella manillensis, and putting down the film shed after feeding; feeding every 4-6 days, selecting 10-12 am to feed, and changing water for the culture pond every 2-3 days through a water outlet pipe and a water inlet pipe.
preferably, the treatment method of the pig blood or the cattle blood as the feed comprises the following steps: naturally coagulating pig blood or cattle blood into blood clots, adding water with the mass 2-3 times that of the blood clots, mixing and crushing to prepare blood plasma, and heating the obtained blood plasma to 37-38 ℃ to obtain the feed.
Preferably, the method further comprises the step of S4 guiding the Poecilobdella manillensis to forage: the side wall of the culture pond is arranged in a zigzag ladder shape, the shell powder coating is coated, a sun shield is fixedly erected above the side wall to form a space for the Poecilobdella manillensis to rest, and then a casing filled with fresh pig and bovine blood is put into the space once a week to guide the Poecilobia manillensis to find food and rest;
The preparation method of the shell powder coating comprises the following steps: mixing 20-30 parts by weight of shell powder and 5-8 parts by weight of bamboo charcoal powder to obtain powder; mixing 2-3 parts by weight of casein glue, 1-2 parts by weight of tortoise-plastron glue, 3-5 parts by weight of sticky rice glue and 0.5-0.8 part by weight of fatty acid methyl ester ethoxylate to obtain latex; and mixing the powder and the latex, adding 6-8 parts by weight of water, and mixing and stirring at 80-100 ℃ for 2-3 hours to obtain the shell powder coating.
preferably, the method further comprises S5 disease management: if the leech has the conditions of inappetence, no desire to move and red and swollen anus, a water source containing 0.08-0.12% of the antibiotics by mass is led into the water inlet pipe, then the water in the culture pond is changed once through the water outlet pipe and the water inlet pipe, and the water without the antibiotics is used for changing the water again after 12-16 hours.
Preferably, the method further comprises the step of oxygenating the S6 culture pond: and starting an air compressor and an air heater at 12 noon every day, heating the air introduced into an inflation pipe laid at the bottom of the culture pond until the air temperature reaches 30-35 ℃, and then inflating the culture pond for 2-4 hours, wherein the inflation amount of each cubic meter of pond water in the culture pond is 5-8L/h.
The invention at least comprises the following beneficial effects:
Firstly, the Poecilobdella manillensis culture equipment detects the temperature of water in a culture pond in real time through a temperature controller, the temperature controller automatically cuts off a heating power supply when the water temperature reaches a set temperature, and the temperature controller automatically communicates with the heating power supply to heat when the water temperature is lower than the set temperature; the cultivation equipment for the poecilobdella manillensis is different from the common indoor cultivation that the water temperature cannot be effectively controlled by covering the heat-insulating plates above the cultivation pool and the spawning platform and using the film shed for overall heat insulation, and is favorable for avoiding the severe fluctuation of the water temperature, the air temperature and the soil temperature, thereby achieving the purposes of saving energy, saving cost and being convenient for manual operation; meanwhile, the heat-insulating mechanism is formed by simply assembling the nut rod, the keel, the heat-insulating plate and the film shed, so that the mounting and the dismounting are very convenient, the heat-insulating mechanism can be dismounted after the Poecilobdella manillensis passes through the winter period, and the heat-insulating mechanism is convenient to mount in the winter period, has the advantages of simple structure, convenience in operation and maintenance, and also has good economic benefit;
secondly, the small tiles are arranged below the stainless steel electric welding net, so that on one hand, the electric welding net can be supported, the situation that the Poecilobdella manillensis is pressed by the electric welding net and cannot move is effectively avoided, on the other hand, the Poecilobdella manillensis can inhabit below the tiles, stress of the Poecilobdella manillensis is reduced, and the survival rate of the Poecilobdella manillensis in overwintering is improved;
thirdly, an inflation pipe is laid at the bottom of the culture pond and is communicated with an air heater and an air compressor, water in the culture pond is oxygenated through a plurality of hollow cylinders with air outlets at the tops of air outlet ends of the inflation pipe, a whole piece of microporous sponge is adhered above the air outlets and covers the tops of the hollow cylinders, and air can be discharged from a net structure in the microporous sponge through the structure of the air outlets and the microporous sponge, so that the contact area of oxygen and pond water is effectively increased, and oxygen dissolution is facilitated; meanwhile, the air introduced into the pond water is heated by the air heater, so that the method is different from a conventional oxygenation method, can effectively prevent low-temperature air from entering the pond water in winter to reduce the temperature of the pond water and reduce heat loss, thereby effectively reducing water temperature fluctuation during oxygenation, facilitating the overwintering of the poecilobdella manillensis and improving the survival rate of the poecilobdella manillensis in the winter;
fourthly, the side wall of the culture pond is arranged in a zigzag ladder shape, the shell powder coating made of shell powder, bamboo charcoal powder, casein glue, tortoise-plastron glue, glutinous rice glue and the like is coated on the side wall, a sun shield is fixedly erected above the side wall to form a space for the poecilobdella manillensis to rest, and then a casing filled with fresh pig and cattle blood is put into the space once a week to guide the poecilobdella manillensis to find food and rest; the sawtoothed stepped side wall is convenient for the poecilobdella manillensis to rest, and the poecilobdella manillensis is coated with the shell powder coating when being sucked and filled into the casing filled with fresh pig and cattle blood, so that the blood is possibly leaked and deteriorated in the sucking process to cause the breeding of harmful bacteria, the shell powder coating is rich in chitin, the biological structure of double helix of the shell powder is not suitable for the growth of bacteria, the shell powder has extremely strong antibacterial and bactericidal effects on escherichia coli, salmonella and yellow glucose bacteria, the death of the poecilobdella manillensis infected by the harmful bacteria is reduced, and the survival rate of the poecilobdella manillensis is improved; the bamboo charcoal powder has countless pores, the carbon pores can effectively adsorb a part of suspended matters in water, the effects of adsorbing, decomposing peculiar smell and deodorizing are achieved on the excrement of Poecilobdella manillensis, and meanwhile, the shell powder and the bamboo charcoal powder have remarkable mildew-proof functions, so that the cleanness of a water body is ensured; the casein glue, the tortoise-plastron glue and the glutinous rice glue are all animal and vegetable glue, so that the influence of using chemical glue on water quality is reduced to a certain extent;
fifthly, the air is heated to 30-35 ℃ and then is introduced into the pond water, so that the temperature of the air is slightly higher than that of the pond water, part of heat of the air can be transferred into the pond water, part of heat dissipated due to air circulation in the pond water in the oxygenation process is offset, the water temperature is more stable, and the survival rate of the Poecilobdella manillensis over-wintering is further improved.
additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
drawings
FIG. 1 is a schematic structural diagram of a Poecilobdella manillensis culture device in the invention;
FIG. 2 is a schematic structural view of a heat retention mechanism according to the present invention;
FIG. 3 is a schematic structural view of a hollow cylinder at the outlet end of the inflation tube of the present invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
< example 1>
a poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
the device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at the pipe orifice of the water outlet pipe, and soil is filled in the spawning platforms 2;
the heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
The method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting 3cm of water into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 23 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 40 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as feed, lifting the film shed to feed Poecilobdella manillensis, and putting down the film shed after feeding; feeding every 4 days, selecting 10 am to feed, and changing water for the culture pond 1 every 2 days through a water outlet pipe and a water inlet pipe.
< example 2>
a poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
The device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at the pipe orifice of the water outlet pipe, and soil is filled in the spawning platforms 2;
the heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting water with the depth of 8cm into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 30 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 80 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
S3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as feed, lifting the film shed to feed Poecilobdella manillensis, and putting down the film shed after feeding; feeding once every 6 days, selecting 12 am to feed, and changing water for the culture pond 1 once every 3 days through a water outlet pipe and a water inlet pipe.
< example 3>
A poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
The device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at the pipe orifice of the water outlet pipe, and soil is filled in the spawning platforms 2;
The heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting water with the depth of 6cm into the culture pond 1, adjusting the temperature of the water 1 in the culture pond through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 26 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 60 pieces of poecilobdella manillensis per square meter are put into a culture pond for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as feed, lifting the film shed to feed Poecilobdella manillensis, and putting down the film shed after feeding; feeding once every 5 days, selecting 11 am to feed, and changing water for the culture pond 1 once every 2 days through a water outlet pipe and a water inlet pipe.
< example 4>
a poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
The device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 5cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel;
The heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
The heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting 3cm of water into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 23 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 40 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass 2 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 37 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding every 4 days, selecting 10 am to feed, and changing water for the culture pond 1 every 2 days through a water outlet pipe and a water inlet pipe.
< example 5>
a poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
the device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 10cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel;
The heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
The heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
The method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting water with the depth of 8cm into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 30 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 80 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass of 3 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 38 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding once every 6 days, selecting 12 am to feed, and changing water for the culture pond 1 once every 3 days through a water outlet pipe and a water inlet pipe.
< example 6>
a poecilobdella manillensis culture device is shown in figures 1-2 and comprises:
the device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 8cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel;
the heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
the heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
S1, preparation before cultivation: putting water with the depth of 6cm into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 26 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 60 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
S3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass 2.5 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 37 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding once every 5 days, selecting 11 am to feed, and changing water for the culture pond 1 once every 2 days through a water outlet pipe and a water inlet pipe.
< example 7>
a Poecilobdella manillensis culture device is shown in figures 1-3 and comprises:
the device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 5cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel; an air charging pipe 16 is further laid at the bottom of the culture pond 1, an air inlet end of the air charging pipe 16 is communicated with an air outlet of an air heater, an air inlet of the air heater is connected with an air compressor, an air outlet end of the air charging pipe 16 is communicated with a plurality of hollow cylinders 17, a plurality of air outlet holes 18 are formed in the tops of the hollow cylinders, a whole piece of microporous sponge 19 is adhered to the upper portions of the air outlet holes and covers the tops of the hollow cylinders, and the microporous sponge 19 is soaked below the liquid level; the side wall of the culture pond 1 close to the spawning platform is in a zigzag ladder shape, a sun shield is fixedly erected above the side wall, the sun shield is parallel to the side wall and shields the side wall to form a space for the poecilobdella manillensis to rest, and the side wall of the culture pond 1 is coated with shell powder coating;
the heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
the heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
The method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
s1, preparation before cultivation: putting 3cm of water into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 23 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 40 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
S3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass 2 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 37 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding every 4 days, selecting 10 am to feed, and changing water for the culture pond 1 every 2 days through a water outlet pipe and a water inlet pipe;
s4, guiding Poecilobdella manillensis to forage: the side wall of the culture pond 1 is arranged in a zigzag ladder shape, the shell powder coating is coated on the side wall, a sun shield is fixedly erected above the side wall to form a space for the Poecilobdella manillensis to rest, and then a casing filled with fresh pig and bovine blood is put into the space once a week to guide the Poecilobdella manillensis to find food and rest; the preparation method of the shell powder coating comprises the following steps: mixing 20 parts by weight of shell powder and 5 parts by weight of bamboo charcoal powder to obtain powder; mixing 2 parts by weight of casein glue, 1 part by weight of tortoise-plastron glue, 3 parts by weight of glutinous rice glue and 0.5 part by weight of fatty acid methyl ester ethoxylate to obtain latex; mixing the powder and the latex, adding 6 parts by weight of water, and then mixing and stirring for 2 hours at 80 ℃ to obtain the shell powder coating; the preparation method of casing filled with fresh pig and cattle blood comprises the following steps: soaking fresh pig casing in oil, washing, inflating, selecting casing without air leakage, air drying, filling fresh pig and cattle blood, and fastening two ends of the casing to obtain casing filled with fresh pig and cattle blood;
s5, disease management: if the leech has the conditions of inappetence, no desire to move and red and swollen anus, introducing a water source containing 0.08 percent of the mass fraction of the antibiotics into the water inlet pipe, then changing the water in the culture pond 1 once through the water outlet pipe and the water inlet pipe, and changing the water again after 12 hours by using the water without the antibiotics;
s6, oxygenation of the culture pond: starting an air compressor and an air heater at 12 noon every day, heating air introduced into an inflation pipe 16 laid in the culture pond 1 until the air temperature reaches 30 ℃, and then inflating the culture pond 1 for 2 hours, wherein the inflation amount of each cubic meter of pond water in the culture pond 1 is 5L/h.
< example 8>
A Poecilobdella manillensis culture device is shown in figures 1-3 and comprises:
the device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 10cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel; an air charging pipe 16 is further laid at the bottom of the culture pond 1, an air inlet end of the air charging pipe 16 is communicated with an air outlet of an air heater, an air inlet of the air heater is connected with an air compressor, an air outlet end of the air charging pipe 16 is communicated with a plurality of hollow cylinders 17, a plurality of air outlet holes 18 are formed in the tops of the hollow cylinders, a whole piece of microporous sponge 19 is adhered to the upper portions of the air outlet holes and covers the tops of the hollow cylinders, and the microporous sponge 19 is soaked below the liquid level; the side wall of the culture pond 1 close to the spawning platform is in a zigzag ladder shape, a sun shield is fixedly erected above the side wall, the sun shield is parallel to the side wall and shields the side wall to form a space for the poecilobdella manillensis to rest, and the side wall of the culture pond 1 is coated with shell powder coating;
The heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
the heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
S1, preparation before cultivation: putting water with the depth of 8cm into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 30 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
s2, putting poecilobdella manillensis: 80 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass of 3 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 38 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding every 6 days, selecting 12 am to feed, and changing water for the culture pond 1 every 3 days through a water outlet pipe and a water inlet pipe;
s4, guiding Poecilobdella manillensis to forage: the side wall of the culture pond 1 is arranged in a zigzag ladder shape, the shell powder coating is coated on the side wall, a sun shield is fixedly erected above the side wall to form a space for the Poecilobdella manillensis to rest, and then a casing filled with fresh pig and bovine blood is put into the space once a week to guide the Poecilobdella manillensis to find food and rest; the preparation method of the shell powder coating comprises the following steps: mixing 30 parts by weight of shell powder and 8 parts by weight of bamboo charcoal powder to obtain powder; mixing 3 parts by weight of casein glue, 2 parts by weight of tortoise-plastron glue, 5 parts by weight of sticky rice glue and 0.8 part by weight of fatty acid methyl ester ethoxylate to obtain latex; mixing the powder and the latex, adding 6-8 parts by weight of water, and then mixing and stirring for 3 hours at 100 ℃ to obtain the shell powder coating; the preparation method of casing filled with fresh pig and cattle blood comprises the following steps: soaking fresh pig casing in oil, washing, inflating, selecting casing without air leakage, air drying, filling fresh pig and cattle blood, and fastening two ends of the casing to obtain casing filled with fresh pig and cattle blood;
s5, disease management: if the leech has the conditions of inappetence, no desire to move and red and swollen anus, introducing a water source containing 0.12 percent of the antibiotic by mass into the water inlet pipe, then changing the water in the culture pond 1 once through the water outlet pipe and the water inlet pipe, and changing the water again by using the water without the antibiotic after 16 hours;
S6, oxygenation of the culture pond: starting an air compressor and an air heater at 12 noon every day, heating air introduced into an inflation pipe 16 laid at the bottom of the culture pond 1 until the air temperature reaches 35 ℃, and then inflating the culture pond 1 for 4 hours, wherein the inflation amount of each cubic meter of pond water in the culture pond 1 is 8L/h.
< example 9>
a Poecilobdella manillensis culture device is shown in figures 1-3 and comprises:
The device comprises a plurality of culture ponds 1 and a plurality of spawning platforms 2 which are alternately arranged, wherein heating cables 11 are uniformly laid at the bottoms of the culture ponds 1, temperature probe lines 12 are arranged below the liquid level of the culture ponds 1, the heating cables 11 and the temperature probe lines 12 are both connected with a temperature controller 3, a water inlet pipe is arranged above the liquid level of the culture ponds 1, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at a pipe orifice of the water outlet pipe, rice field soil, coarse sand or gravel with the thickness of 8cm is filled in the spawning platforms 2, and jute bags are laid on the rice field soil, the coarse sand or the gravel; an air charging pipe 16 is further laid at the bottom of the culture pond 1, an air inlet end of the air charging pipe 16 is communicated with an air outlet of an air heater, an air inlet of the air heater is connected with an air compressor, an air outlet end of the air charging pipe 16 is communicated with a plurality of hollow cylinders 17, a plurality of air outlet holes 18 are formed in the tops of the hollow cylinders, a whole piece of microporous sponge 19 is adhered to the upper portions of the air outlet holes and covers the tops of the hollow cylinders, and the microporous sponge 19 is soaked below the liquid level; the side wall of the culture pond 1 close to the spawning platform is in a zigzag ladder shape, a sun shield is fixedly erected above the side wall, the sun shield is parallel to the side wall and shields the side wall to form a space for the poecilobdella manillensis to rest, and the side wall of the culture pond 1 is coated with shell powder coating;
the heating cable 11 is bound with a stainless steel electric welding net 14 through a nylon tie 13, broken tiles 15 are placed below the stainless steel electric welding net 14, the temperature controller 3 is installed in an electric meter box 31 and connected with an air switch 32 in the electric meter box 31, the air switch 32 is connected with a bidirectional knife switch 33 through a copper wire 34, and the bidirectional knife switch 33 is connected with a power supply line 35 and a standby power supply line 36;
The heat preservation mechanism is erected above the culture pond 1 and the spawning platform 2, and is provided with nut rods 4, keels 5, a heat preservation plate 6 and a film shed from bottom to top, the nut rods 4 are arranged on the peripheries of the culture pond 4 and the spawning platform 2 in a plurality of rows and columns along the vertical direction, each keel 5 comprises a main keel 51 and an auxiliary keel 52 which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate 6 is erected above the keel 5, and the heat preservation plate 6 and the nut rods 4 are covered by the film shed from top to bottom;
The method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment comprises the following steps:
S1, preparation before cultivation: putting water with the depth of 6cm into the culture pond 1, adjusting the water temperature of the culture pond 1 through a temperature controller 3 and a heating cable 11, controlling the water temperature to be 26 ℃, building a heat preservation mechanism, filling soil into the spawning platform 2, and paving jute bags on the surface of the soil;
S2, putting poecilobdella manillensis: 60 pieces of poecilobdella manillensis per square meter are put into the culture pond 1 for overwintering period culture;
S3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as a feed, naturally coagulating the pig blood or cattle blood into blood clots, adding water with the mass 2.5 times that of the blood clots, mixing and crushing the mixture to prepare blood plasma, and heating the obtained blood plasma to 37 ℃ to prepare the feed; then lifting the film shed to feed the poecilobdella manillensis, and putting down the film shed after feeding; feeding every 5 days, selecting 11 am to feed, and changing water for the culture pond 1 every 2 days through a water outlet pipe and a water inlet pipe;
s4, guiding Poecilobdella manillensis to forage: the side wall of the culture pond 1 is arranged in a zigzag ladder shape, the shell powder coating is coated on the side wall, a sun shield is fixedly erected above the side wall to form a space for the Poecilobdella manillensis to rest, and then a casing filled with fresh pig and bovine blood is put into the space once a week to guide the Poecilobdella manillensis to find food and rest; the preparation method of the shell powder coating comprises the following steps: mixing 25 parts by weight of shell powder and 6 parts by weight of bamboo charcoal powder to obtain powder; mixing 2.5 parts by weight of casein glue, 1.5 parts by weight of tortoise-plastron glue, 4 parts by weight of sticky rice glue and 0.6 part by weight of fatty acid methyl ester ethoxylate to obtain latex; mixing the powder and the latex, adding 7 parts by weight of water, and then mixing and stirring at 90 ℃ for 2.5 hours to obtain the shell powder coating; the preparation method of casing filled with fresh pig and cattle blood comprises the following steps: soaking fresh pig casing in oil, washing, inflating, selecting casing without air leakage, air drying, filling fresh pig and cattle blood, and fastening two ends of the casing to obtain casing filled with fresh pig and cattle blood;
s5, disease management: if the leech has the conditions of inappetence, no desire to move and red and swollen anus, introducing a water source containing 0.1 percent of the mass fraction of the antibiotics into the water inlet pipe, then changing the water in the culture pond 1 once through the water outlet pipe and the water inlet pipe, and changing the water again after 14 hours by using the water without the antibiotics;
s6, oxygenation of the culture pond: starting an air compressor and an air heater at 12 noon every day, heating air introduced into an inflation pipe 16 laid at the bottom of the culture pond 1 until the air temperature reaches 32 ℃, and then inflating the culture pond 1 for 3 hours, wherein the inflation amount of each cubic meter of pond water in the culture pond 1 is 6.5L/h.
< comparative test >
The poecilobdella manillensis of the same variety and the same size as those in the embodiments 1-9 is selected and put into an indoor overwintering culture pond (without an automatic water temperature control function, a spawning platform and a heat preservation mechanism built by a nut rod, a keel, a heat preservation plate and a film shed) according to the density of 60 poecilobdella manillensis per square meter for comparison test, the indoor temperature is 26 ℃, the water depth of the culture pond is 6cm, and the commercial conventional feed (feed containing grains) is fed according to a conventional method to serve as a comparative example 1; selecting the Poecilobdella manillensis cultivation equipment in the embodiment 3, selecting Poecilobdella manillensis of the same variety and the same size as the Poecilobdella manillensis in the comparative example 1, putting the Poecilobdella manillensis in the cultivation density of 60 Poecilobdella manillensis per square meter for a comparative test, feeding the Poecilobdella manillensis with a conventional feed (feed containing grains of five cereals) sold on the market, and feeding the Poecilobdella manillensis with the; the poecilobdella manillensis of the same variety and size as those in comparative example 1 is selected and put into an indoor overwintering culture pond (without an automatic water temperature control function, a spawning platform and a heat preservation mechanism built by a nut rod, a keel, a heat preservation plate and a film shed) according to the density of 60 poecilobdella manillensis per square meter for comparison test, and fed according to the culture method in example 3 (except that the water temperature control is not included, the other methods are consistent) to serve as comparative example 3; comparative examples 1 to 3 were compared with the cultivated Poecilobdella manillensis of examples 1 to 9, as shown in the following table:
TABLE 1 comparative examples 1 to 3 to comparative examples 1 to 9
| Survival rate of overwintering (%) | weight gain after overwintering (%) | |
| Comparative example 1 | 85.6 | 14.7 |
| comparative example 2 | 85.8 | 14.9 |
| Comparative example 3 | 85.4 | 14.4 |
| Example 1 | 95.4 | 23.2 |
| example 2 | 95.5 | 23.5 |
| Example 3 | 95.7 | 23.8 |
| Example 4 | 96.2 | 24.6 |
| example 5 | 96.5 | 24.9 |
| Example 6 | 96.7 | 25.2 |
| Example 7 | 97.8 | 26.2 |
| Example 8 | 98.1 | 26.5 |
| Example 9 | 98.5 | 26.7 |
as can be seen from Table 1, the survival rate of Poecilobdella manillensis cultivated by the equipment and the method in the embodiments 1-3 of the invention reaches 95.4% -95.7%, which is higher than the survival rate of 85.4% -85.8% in the comparative examples 1-3; after overwintering, the weight increase of the poecilobdella manillensis in the embodiments 1-3 reaches 23.2% -23.8%, and is also higher than the weight increase of 14.4% -14.9% in the comparative examples 1-3, which shows that the poecilobdella manillensis culture equipment and the method for culturing the poecilobdella manillensis through the poecilobdella manillensis culture equipment effectively improve the survival rate of the poecilobdella manillensis during the overwintering period and ensure the normal growth of the poecilobdella manillensis; the survival rate of the poecilobdella manillensis cultivated by the equipment and the method in the embodiments 4-6 of the invention reaches 95.7-96.5 percent, the weight gain reaches 24.6-25.2 percent, and the survival rate is partially improved compared with the survival rate in the embodiments 1-3; the survival rate of the poecilobdella manillensis cultivated by the equipment and the method in the embodiments 7-9 reaches 97.8-98.5% and the weight gain reaches 26.2-26.7%, and is obviously improved compared with the devices in the embodiments 1-6, which shows that the survival rate of the poecilobdella manillensis during the overwintering period is further improved and the normal growth of the poecilobdella manillensis is ensured by a series of steps of placing broken tiles below a stainless steel electric welding net, paving an air charging pipe at the bottom of a pond, heating air by using an air heater, oxygenating pond water by combining a hollow cylinder and a microporous sponge, setting the side wall of the cultivating pond into a zigzag ladder shape, coating shell powder on the side wall of the cultivating pond and the like.
Meanwhile, the examination shows that about 90.2% -93.5% of the sex glands of the poecilobdella manillensis in the examples 1-9 are full, and the egg laying and breeding of the poecilobdella manillensis are facilitated.
while embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the invention, and further modifications may readily be effected by those skilled in the art, so that the invention is not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (4)
1. The method for cultivating Poecilobdella manillensis by applying Poecilobdella manillensis cultivation equipment in the overwintering way is characterized in that,
the breeding equipment for poecilobdella manillensis comprises:
The method comprises the following steps that a plurality of culture ponds and a plurality of spawning platforms are alternately arranged, soil filled in the spawning platforms is rice field soil, coarse sand or gravel with the thickness of 5-10 cm, and gunny bags are laid on the rice field soil, the coarse sand or the gravel; heating cables are uniformly laid at the bottom of the culture pond, a temperature probe line is arranged below the liquid surface of the culture pond, an inflation tube is laid at the bottom of the culture pond, the air inlet end of the inflation tube is communicated with the air outlet of an air heater, the air inlet of the air heater is connected with an air compressor, the air outlet end of the inflation tube is communicated with a plurality of hollow cylinders, a plurality of air outlet holes are formed in the tops of the hollow cylinders, a whole piece of microporous sponge is adhered to the upper parts of the air outlet holes and covers the tops of the hollow cylinders, and the microporous sponge is immersed below the liquid level; the heating cable and the temperature probe line are both connected with a temperature controller, a water inlet pipe is arranged above the liquid level of the culture pond, a water outlet pipe is arranged below the liquid level, an anti-escape net is arranged at the pipe orifice of the water outlet pipe, and soil is filled in the spawning platform; the heating cable is bound with a stainless steel electric welding net through a nylon ribbon, broken tiles are placed below the stainless steel electric welding net, the temperature controller is installed in an electric meter box and connected with an air switch in the electric meter box, the air switch is connected with a bidirectional knife switch through a copper wire, and the bidirectional knife switch is connected with a power supply line and a standby power supply line;
the heat preservation mechanism is erected above the culture pond and the spawning platform, and is provided with nut rods, keels, a heat preservation plate and a film shed from bottom to top, the nut rods are arranged on the peripheries of the culture pond and the spawning platform in a plurality of rows and columns along the vertical direction, the keels comprise main keels and auxiliary keels which are positioned on the same horizontal plane and are mutually perpendicular and clamped, the heat preservation plate is erected above the keels, and the heat preservation plate and the nut rods are covered by the film shed from top to bottom; the side wall of the culture pond close to the spawning platform is in a zigzag ladder shape, a sun shield is fixedly erected above the side wall, the sun shield is parallel to the side wall and shields the side wall to form a space for the poecilobdella manillensis to rest, and the side wall of the culture pond is coated with shell powder coating;
the method for culturing Poecilobdella manillensis in the overwintering mode comprises the following steps:
s1, preparation before cultivation: putting 3-8 cm of water into a culture pond, adjusting the water temperature of the culture pond through a temperature controller and a heating cable, controlling the water temperature to be 23-30 ℃, building a heat preservation mechanism, filling soil into a spawning platform, and paving jute bags on the surface of the soil;
s2, putting poecilobdella manillensis: putting 40-80 Poecilobdella manillensis per square meter into a culture pond for overwintering period culture;
s3, daily management of Poecilobdella manillensis: selecting pig blood or cattle blood as feed, lifting the film shed to feed Poecilobdella manillensis, and putting down the film shed after feeding; feeding every 4-6 days, selecting 10-12 am to feed, and changing water for the culture pond every 2-3 days through a water outlet pipe and a water inlet pipe;
s4, guiding Poecilobdella manillensis to forage: the side wall of the culture pond is arranged in a zigzag ladder shape, the shell powder coating is coated, a sun shield is fixedly erected above the side wall to form a space for the Poecilobdella manillensis to rest, and then a casing filled with fresh pig and bovine blood is put into the space once a week to guide the Poecilobia manillensis to find food and rest; the preparation method of the shell powder coating comprises the following steps: mixing 20-30 parts by weight of shell powder and 5-8 parts by weight of bamboo charcoal powder to obtain powder; mixing 2-3 parts by weight of casein glue, 1-2 parts by weight of tortoise-plastron glue, 3-5 parts by weight of sticky rice glue and 0.5-0.8 part by weight of fatty acid methyl ester ethoxylate to obtain latex; and mixing the powder and the latex, adding 6-8 parts by weight of water, and mixing and stirring at 80-100 ℃ for 2-3 hours to obtain the shell powder coating.
2. the method for overwintering culture of Poecilobdella manillensis by using Poecilobdella manillensis culture equipment as claimed in claim 1, wherein the pig blood or the cattle blood is used as feed by a processing method comprising the following steps: naturally coagulating pig blood or cattle blood into blood clots, adding water with the mass 2-3 times that of the blood clots, mixing and crushing to prepare blood plasma, and heating the obtained blood plasma to 37-38 ℃ to obtain the feed.
3. the method for overwintering cultivation of Poecilobdella manillensis by using Poecilobdella manillensis cultivation equipment as claimed in claim 1, further comprising S5 disease management: if the leech has the conditions of inappetence, no desire to move and red and swollen anus, a water source containing 0.08-0.12% of the antibiotics by mass is led into the water inlet pipe, then the water in the culture pond is changed once through the water outlet pipe and the water inlet pipe, and the water without the antibiotics is used for changing the water again after 12-16 hours.
4. the method for overwintering culture of Poecilobdella manillensis by using Poecilobdella manillensis culture equipment as claimed in claim 1, further comprising the step of oxygenating the culture pond S6: and starting an air compressor and an air heater at 12 noon every day, heating the air introduced into an inflation pipe laid at the bottom of the culture pond until the air temperature reaches 30-35 ℃, and then inflating the culture pond for 2-4 hours, wherein the inflation amount of each cubic meter of pond water in the culture pond is 5-8L/h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611207522.4A CN106688988B (en) | 2016-12-23 | 2016-12-23 | Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611207522.4A CN106688988B (en) | 2016-12-23 | 2016-12-23 | Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106688988A CN106688988A (en) | 2017-05-24 |
| CN106688988B true CN106688988B (en) | 2019-12-10 |
Family
ID=58903719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611207522.4A Expired - Fee Related CN106688988B (en) | 2016-12-23 | 2016-12-23 | Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106688988B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197747A (en) * | 2017-07-03 | 2019-01-15 | 广元公正科技有限公司 | The equipment that cold place bummaree heats the water in fishpond |
| CN108094274A (en) * | 2017-11-30 | 2018-06-01 | 广西大学 | A kind of cultural method of hiruto |
| CN108566908A (en) * | 2017-12-19 | 2018-09-25 | 江苏农牧科技职业学院 | Leech, Mixed cultivation of prawn and crab cultivate factory |
| CN108967284A (en) * | 2018-07-09 | 2018-12-11 | 重庆多普泰生物科技开发有限责任公司 | A kind of wintering method of Hirudo japonica |
| CN108990849A (en) * | 2018-07-09 | 2018-12-14 | 重庆多普泰生物科技开发有限责任公司 | A kind of wintering pond improving Hirudo japonica survival rate |
| CN109197706A (en) * | 2018-09-25 | 2019-01-15 | 重庆铭实农业科技开发有限责任公司 | A kind of eurysome golden thread leech winter low temperature conservation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820589A (en) * | 2006-03-13 | 2006-08-23 | 孙庆海 | Automatic controller for temperature of fry nursing pool |
| CN102657138A (en) * | 2012-05-16 | 2012-09-12 | 句容市润泰生物科技有限公司 | Leech seeding and farming system |
| CN202514434U (en) * | 2012-03-16 | 2012-11-07 | 大连东泽海产品有限公司 | Oxygen increasing equipment for holothurian culture pond |
| CN104663307A (en) * | 2015-03-17 | 2015-06-03 | 韩承海 | Novel agricultural livable eco system |
| CN106234311A (en) * | 2016-07-27 | 2016-12-21 | 天津鸿腾水产科技发展有限公司 | A kind of hirudiniculture equipment and cultural method |
-
2016
- 2016-12-23 CN CN201611207522.4A patent/CN106688988B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820589A (en) * | 2006-03-13 | 2006-08-23 | 孙庆海 | Automatic controller for temperature of fry nursing pool |
| CN202514434U (en) * | 2012-03-16 | 2012-11-07 | 大连东泽海产品有限公司 | Oxygen increasing equipment for holothurian culture pond |
| CN102657138A (en) * | 2012-05-16 | 2012-09-12 | 句容市润泰生物科技有限公司 | Leech seeding and farming system |
| CN104663307A (en) * | 2015-03-17 | 2015-06-03 | 韩承海 | Novel agricultural livable eco system |
| CN106234311A (en) * | 2016-07-27 | 2016-12-21 | 天津鸿腾水产科技发展有限公司 | A kind of hirudiniculture equipment and cultural method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106688988A (en) | 2017-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106688988B (en) | Poecilobdella manillensis culture equipment and method for overwintering culture of Poecilobdella manillensis by using same | |
| CN107114328B (en) | A kind of black soldier flies cultivating system and cultural method | |
| CN101766154B (en) | Method for high-density green ecological culture of blood-sucking leeches | |
| CN103937668B (en) | Nostoc Algal Ecology simulation culture systems and cultural method | |
| CN101897321A (en) | Quick scorpion breeding without hibernation | |
| CN104430125A (en) | Efficient aquaculture system and method | |
| CN104255671B (en) | Brachionus plicatilis artificial water circulation high-yield cultivating system and breeding method | |
| CN105432181A (en) | Method for promoting germination of seed of gomuti palm | |
| CN207167471U (en) | Giant salamander culture pond | |
| CN107027714A (en) | A kind of edible earthworm indoor culture method | |
| CN102106285A (en) | Kishinouyea pond three-harvest raising technology | |
| CN102599107A (en) | Novel scorpion breeding method | |
| CN104823650B (en) | Water-breeding planting method of water celeries with green and yellow buds | |
| CN108575636A (en) | The breeding method of peanut sprout | |
| CN204305862U (en) | A kind of high-efficiency aquaculture system | |
| CN207040585U (en) | A kind of mud crab spawning sand table | |
| CN1430885A (en) | Method for rearing young silkworm in constant temperature and humidity | |
| CN208639365U (en) | A kind of device suitable for trypetid class insect reproduction | |
| CN106538459A (en) | A kind of cultural method of goat | |
| CN107155983B (en) | High-survival-rate leech culture method | |
| CN109429895A (en) | The method for preparing cover soil material plantation Dictyophora rubrovalvata using waste edible fungus chaff | |
| CN114651763A (en) | Breed conservation method for scylla paramamosain | |
| CN108935246A (en) | A kind of cuttlefish nursery living bait collection and indoor method for temporarily cultivating | |
| CN205284678U (en) | Device is raised to red line worm | |
| CN205106025U (en) | Portable artificial propagation loach fry hatching apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191210 Termination date: 20211223 |