CN109197706A - A kind of eurysome golden thread leech winter low temperature conservation method - Google Patents
A kind of eurysome golden thread leech winter low temperature conservation method Download PDFInfo
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- CN109197706A CN109197706A CN201811112002.4A CN201811112002A CN109197706A CN 109197706 A CN109197706 A CN 109197706A CN 201811112002 A CN201811112002 A CN 201811112002A CN 109197706 A CN109197706 A CN 109197706A
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- golden thread
- thread leech
- eurysome golden
- overwintering
- leech
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- 241000545744 Hirudinea Species 0.000 title claims abstract description 122
- 241000099774 Cuscuta salina Species 0.000 title claims abstract description 97
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000004083 survival effect Effects 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims abstract description 7
- 239000004576 sand Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000218202 Coptis Species 0.000 claims description 11
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 7
- 239000011449 brick Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000010865 sewage Substances 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- 239000013589 supplement Substances 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 210000004681 ovum Anatomy 0.000 claims description 4
- 238000012795 verification Methods 0.000 claims description 4
- 230000003442 weekly effect Effects 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 description 9
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 241000254223 Syntexis Species 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000243818 Annelida Species 0.000 description 1
- 241001123468 Arhynchobdellida Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
A kind of eurysome golden thread leech conservation method, in particular to eurysome golden thread leech winter low temperature protecting method.The present invention solves habitat and heat preservation, the novel designs etc. of overwintering container, so that eurysome golden thread leech Overwintering Survival Percentage greatly improves, and thin rate reduces etc. by changing the external environment influence overwintering to eurysome golden thread leech.The method of the present invention is furtherd investigate and is innovated to the overwintering technology of eurysome golden thread leech; kind source problem increasingly in short supply and constantly degenerating in eurysome golden thread leech breeding process to solve scale; it is simultaneously numerous raisers' save the cost; profit space is created, this has great importance to the status for improving eurysome golden thread leech shortage of resources.
Description
One, technical field
The invention belongs to eurysome golden thread leech technical fields, in particular to eurysome golden thread leech winter low temperature protecting method.
Two, technical background
Eurysome golden thread leech category Annelida, Hirudinea, Arhynchobdellae, Huang Zhi section gold thread leech category, nickname leech are that China passes
Unite Chinese medicine, have eliminating stasis to resolve swelling, evict extravesated blood, eliminating inflammation and expelling toxin and other effects from, be mainly used in clinic treat cardiovascular disease,
The diseases such as hyperlipidemia, gynaecology, andrology, internal medicine.
Due to eurysome golden thread leech medical value with higher and economic value, so that pharmaceuticals industry needs eurysome golden thread leech
It asks and increases year after year, wild leech is influenced by pollutions such as pesticide, chemical fertilizer, trade wastes in recent years, along with artificially to expanded letter
The immoderate fishing of gold thread leech causes wild leech resource almost exhausted, and insufficiency of supply-demand increasingly widens, so eurysome golden thread leech
Artificial breeding industry is forced gradually to rise.But current cultural technique is not yet mature, and aquaculture cost is high, and profit space is narrow, main
Based on the cultivation of casual household's small area, greatly all in the experimental exploring stage, inconvenience is promoted.Limit eurysome golden thread leech artificial breeding extremely
One of important reason is exactly kind of a source problem.Majority provenances are caught from the field in spring at present, with the reduction of wild resource,
The price of kind of leech is higher and higher, for the overwintering survival rate for reducing whole aquaculture cost, improving eurysome golden thread leech, based on ensuring expanded letter
The demand of gold thread leech kind source stability and the screening of breeding leech.
Summarizing prior art disadvantage is 1, traditional eurysome golden thread leech wintering method, and survival rate is low and unstable (survival rate
Fluctuated in 40-70%), be affected by the external environment big (such as temperature jump bust, SEA LEVEL VARIATION, noise effect, vibration influence etc.).
2, traditional eurysome golden thread leech wintering method, leech syntexis ratio is high, and weight syntexis rate every year on average is all 40%.3, traditional
Eurysome golden thread leech wintering method collect when high labor cost, it is larger to the stimulation of eurysome golden thread leech, and do not adapt to produce cocoon
Environment causes the second link death rate high.
Three, summary of the invention
The purpose of the present invention is the overwintering survival rate to reduce whole aquaculture cost, improving eurysome golden thread leech, based on ensuring
The demand of eurysome golden thread leech kind source stability and the screening of breeding leech.
The principle of the present invention is as follows: belonging to poikilotherm based on eurysome golden thread leech, does not feed when water temperature is lower than 15 DEG C, water
Temperature enters hibernation-like state when being lower than 10 DEG C and lacks under movable basic premise, and expanded letter gold thread is influenced by manual control environment's temperature
The body temperature of leech makes it that metabolism be greatly reduced, steadily in the long term in hibernation-like state without being influenced by environmental stimuli.Due to expanded letter gold
Line leech is overwintering gregarious phenomenon, and likes dark narrow gap, therefore be arranged in each wintering pond according to this characteristic
Portion's layout.Adaptation according to biology to Periodical, many biologies are developed in varying temperature environment than in isoperibol more preferable
It (such as plant in alternating temperature round the clock, grows, blossom and bear fruit and product quality is improved.Temperature change is to fish minimum sexal maturity
Age, breeding period, hatching rate etc.), eurysome golden thread leech is solved in this, as theoretical basis does not produce cocoon under artificial overwintering environment
Technical problem.
The object of the present invention is achieved like this: a kind of eurysome golden thread leech winter low temperature conservation method, for the prior art
The shortcomings that, by changing the external environment influence overwintering to eurysome golden thread leech, solve habitat and heat preservation, the novelty of overwintering container
Design etc., so that eurysome golden thread leech Overwintering Survival Percentage greatly improves, and thin rate reduces etc..Its specific method and step is such as
Under:
(1) novel overwintering mode is designed
1) it reduces external environment to set out to the angle of the overwintering interference of eurysome golden thread leech, environment overwintering outside conventional chamber is switched to surely
Fixed indoor environment.
2) leech is caused not enter hibernation-like state to avoid the liter suddenly of temperature from neglecting drop, it is desirable that overwintering indoor environment temperature is answered
Continually and steadily in most suitable 6-10 DEG C of the overwintering temperature of eurysome golden thread leech.
3) from the point of view of solving eurysome golden thread leech habitat and heat preservation, the mixture of traditional soil and brick is changed
For fine sand or square block sponge.
4) from the angle for reducing cost of labor, fine sand or square block sponge are packed into the permeable commercially available woven bag of energy
In, it had not only created the gap that eurysome golden thread leech is hidden but also had accelerated the speed of cleaning and avoid damage to.
5) from the point of view of solving bottom sewage, by overwintering Vessel Design be can replace the circulation of bottom water body into
Row.
(2) new ant algorithms effect is verified
1) overwintering container prepares
It is constant in 5.5-6.5 DEG C of freezer in same temperature, place this overwintering experimental group of at least nine.Container rule
Lattice are 0.4-0.8 cubic metres, and the square block sponge of diameter 2-5mm fine sand or side length 1-2cm are fitted into commercially available woven bag, then
It is put into containers for future use.
2) it is transferred to eurysome golden thread leech
Every group of preparation first is uniform in size, the identical adult eurysome golden thread leech 400-600 item in source.Then 0.1- is used
0.2% strong body stress clever solution eurysome golden thread leech is carried out after impregnating 1-2 hours, then add ice cube in the solution, make water temperature
Freezer steady temperature is progressivelyed reach in 6-8h.Finally just eurysome golden thread leech is transferred in commercially available woven bag, experimental period is 3-4
Month.
3) eurysome golden thread leech is produced
It is adjusted before will producing eurysome golden thread leech first by 1-2 times weekly temperature, within 30-45 days time gradually
Keep eurysome golden thread leech environment temperature identical as room temperature.Then it is transferred to again to eurysome golden thread leech supplement screw and produces cocoon pond.
4) verification result
Finally discovery can relatively efficiently improve eurysome golden thread leech Overwintering Survival Percentage to 70% or more, and expanded letter gold thread
Leech, can be with the effective ovum cocoon of output after being transferred to production cocoon pond.
The present invention is after adopting the above technical scheme, mainly have the following effects:
1, the new ant algorithms that the method for the present invention provides can effectively improve eurysome golden thread leech Overwintering Survival Percentage, and reduce and disappear
Production cocoon quality amount has been effectively ensured in thin rate.
2, the method for the present invention is reducing every production cost compared with traditional mode, be enterprise be raiser saved at
This.
3, the present invention in process of production, is not related to expensive equipment and instrument, thus production equipment is simple, easy to operate
And it is easily controllable, therefore production safety reduces production cost again.
It is furtherd investigate and is innovated using overwintering technology of the method for the present invention to eurysome golden thread leech, to solve scale
Eurysome golden thread leech breeding process in the increasingly in short supply and kind source problem constantly degenerated, while being numerous raisers' save the cost,
Profit space is created, this has great importance to the status for improving eurysome golden thread leech shortage of resources.
Four, specific embodiment
With reference to embodiment, the present invention is further illustrated.
Embodiment 1
A kind of eurysome golden thread leech winter low temperature conservation method, steps are as follows for specific method:
(1) novel overwintering mode is designed
1) it reduces external environment to set out to the angle of the overwintering interference of eurysome golden thread leech, environment overwintering outside conventional chamber is switched to surely
Fixed indoor environment.
2) leech is caused not enter hibernation-like state to avoid the liter suddenly of temperature from neglecting drop, it is desirable that overwintering indoor environment temperature is answered
Continually and steadily in most suitable 6 DEG C of the overwintering temperature of eurysome golden thread leech.
3) from the point of view of solving eurysome golden thread leech habitat and heat preservation, the mixture of traditional soil and brick is changed
Fine sand.
4) from the angle for reducing cost of labor, fine sand or square block sponge are packed into the permeable commercially available woven bag of energy
In, it had not only created the gap that eurysome golden thread leech is hidden but also had accelerated the speed of cleaning and avoid damage to.
5) from the point of view of solving bottom sewage, by overwintering Vessel Design be can replace the circulation of bottom water body into
Row.
(2) new ant algorithms effect is verified
1) overwintering container prepares
It is constant in 5.5 DEG C of freezer in same temperature, this overwintering experimental group of at least nine is placed, containers size is
0.4 cubic metre, diameter 2-5mm fine sand is fitted into commercially available woven bag, containers for future use is placed into.
2) it is transferred to eurysome golden thread leech
Every group of preparation first be uniform in size, the identical adult eurysome golden thread leech in source 400.Then using 0.1% it is strong
Body stress clever solution eurysome golden thread leech is carried out after impregnating 1 hour, then add ice cube in the solution, progressively reach water temperature in 6h
Freezer steady temperature.Finally just eurysome golden thread leech is transferred in commercially available woven bag, experimental period is 3 months.
3) eurysome golden thread leech is produced
It is adjusted before will producing eurysome golden thread leech first by temperature 1 times a week, gradually made width within 30 day time
Body gold thread leech environment temperature is identical as room temperature.Then it is transferred to again to eurysome golden thread leech supplement screw and produces cocoon pond.
Embodiment 2
A kind of eurysome golden thread leech winter low temperature conservation method, steps are as follows for specific method:
(1) novel overwintering mode is designed
1) it reduces external environment to set out to the angle of the overwintering interference of eurysome golden thread leech, environment overwintering outside conventional chamber is switched to surely
Fixed indoor environment.
2) leech is caused not enter hibernation-like state to avoid the liter suddenly of temperature from neglecting drop, it is desirable that overwintering indoor environment temperature is answered
Continually and steadily in most suitable 8 DEG C of the overwintering temperature of eurysome golden thread leech.
3) from the point of view of solving eurysome golden thread leech habitat and heat preservation, the mixture of traditional soil and brick is changed
For fine sand.
4) from the angle for reducing cost of labor, fine sand or square block sponge are packed into the permeable commercially available woven bag of energy
In, it had not only created the gap that eurysome golden thread leech is hidden but also had accelerated the speed of cleaning and avoid damage to.
5) from the point of view of solving bottom sewage, by overwintering Vessel Design be can replace the circulation of bottom water body into
Row.
(2) new ant algorithms effect is verified
1) overwintering container prepares
It is constant in 6.0 DEG C of freezer in same temperature, this overwintering experimental group of at least nine is placed, containers size is
0.6 cubic metre, diameter 2-5mm fine sand is fitted into commercially available woven bag, containers for future use is placed into.
2) it is transferred to eurysome golden thread leech
Every group of preparation first be uniform in size, the identical adult eurysome golden thread leech in source 500.Then using 0.15% it is strong
Body stress clever solution eurysome golden thread leech is carried out after impregnating 1.5 hours, then add ice cube in the solution, reach water temperature gradually in 7h
To freezer steady temperature.Finally just eurysome golden thread leech is transferred in commercially available woven bag, experimental period is 3.5 months.
3) eurysome golden thread leech is produced
It is adjusted before will producing eurysome golden thread leech first by temperature 1 times a week, gradually made width within 35 day time
Body gold thread leech environment temperature is identical as room temperature.Then it is transferred to again to eurysome golden thread leech supplement screw and produces cocoon pond.
4) verification result
Finally discovery can relatively efficiently improve eurysome golden thread leech Overwintering Survival Percentage to 70% or more, and expanded letter gold thread
Leech, can be with the effective ovum cocoon of output after being transferred to production cocoon pond.
Embodiment 3
A kind of eurysome golden thread leech winter low temperature conservation method, steps are as follows for specific method:
(1) novel overwintering mode is designed
1) it reduces external environment to set out to the angle of the overwintering interference of eurysome golden thread leech, environment overwintering outside conventional chamber is switched to surely
Fixed indoor environment.
2) leech is caused not enter hibernation-like state to avoid the liter suddenly of temperature from neglecting drop, it is desirable that overwintering indoor environment temperature is answered
Continually and steadily in most suitable 10 DEG C of the overwintering temperature of eurysome golden thread leech.
3) from the point of view of solving eurysome golden thread leech habitat and heat preservation, the mixture of traditional soil and brick is changed
For square block sponge.
4) from the angle for reducing cost of labor, fine sand or square block sponge are packed into the permeable commercially available woven bag of energy
In, it had not only created the gap that eurysome golden thread leech is hidden but also had accelerated the speed of cleaning and avoid damage to.
5) from the point of view of solving bottom sewage, by overwintering Vessel Design be can replace the circulation of bottom water body into
Row.
(2) new ant algorithms effect is verified
1) overwintering container prepares
It is constant in 6.5 DEG C of freezer in same temperature, this overwintering experimental group of at least nine is placed, containers size is
0.8 cubic metre, the square block sponge of side length 1-2em is fitted into commercially available woven bag, containers for future use is placed into.
2) it is transferred to eurysome golden thread leech
Every group of preparation first be uniform in size, the identical adult eurysome golden thread leech in source 600.Then using 0.2% it is strong
Body stress clever solution eurysome golden thread leech is carried out after impregnating 2 hours, then add ice cube in the solution, progressively reach water temperature in 8h
Freezer steady temperature.Finally just eurysome golden thread leech is transferred in commercially available woven bag, experimental period is 4 months.
3) eurysome golden thread leech is produced
It is adjusted before will producing eurysome golden thread leech first by temperature 2 times a week, gradually made width within 45 day time
Body gold thread leech environment temperature is identical as room temperature.Then it is transferred to again to eurysome golden thread leech supplement screw and produces cocoon pond.
4) verification result
Finally discovery can relatively efficiently improve eurysome golden thread leech Overwintering Survival Percentage to 70% or more, and expanded letter gold thread
Leech, can be with the effective ovum cocoon of output after being transferred to production cocoon pond.
Claims (1)
1. a kind of eurysome golden thread leech winter low temperature conservation method, it is characterised in that steps are as follows for specific method:
(1) novel overwintering mode is designed
1) it reduces external environment to set out to the angle of the overwintering interference of eurysome golden thread leech, environment overwintering outside conventional chamber be switched to stable
Indoor environment；
2) leech is caused not enter hibernation-like state to avoid the liter suddenly of temperature from neglecting drop, it is desirable that overwintering indoor environment temperature should continue
Stablize most suitable 6-10 DEG C of the overwintering temperature in eurysome golden thread leech；
3) from the point of view of solving eurysome golden thread leech habitat and heat preservation, the mixture of traditional soil and brick is changed to carefully
Husky or square block sponge；
4) from the angle for reducing cost of labor, fine sand or square block sponge are fitted into the permeable commercially available woven bag of energy,
Not only it had created the gap that eurysome golden thread leech is hidden but also had accelerated the speed of cleaning and avoid damage to；
It 5) is that can replace the circulation of bottom water body into row by overwintering Vessel Design from the point of view of solving bottom sewage；
(2) new ant algorithms effect is verified
1) overwintering container prepares
It is constant in 5.5-6.5 DEG C of freezer in same temperature, place this overwintering experimental group of at least nine；Containers size is
0.4-0.8 cubic metres, the square block sponge of diameter 2-5mm fine sand or side length 1-2cm are fitted into commercially available woven bag, placed into
Containers for future use；
2) it is transferred to eurysome golden thread leech
Every group of preparation first is uniform in size, the identical adult eurysome golden thread leech 400-600 item in source；Then 0.1-0.2% is used
Strong body stress clever solution eurysome golden thread leech is carried out after impregnating 1-2 hours, then add ice cube in the solution, make water temperature in 6-8h
Progressively reach freezer steady temperature；Finally just eurysome golden thread leech is transferred in commercially available woven bag, experimental period is 3-4 months；
3) eurysome golden thread leech is produced
It is adjusted before will producing eurysome golden thread leech first by 1-2 times weekly temperature, gradually made width within 30-45 days time
Body gold thread leech environment temperature is identical as room temperature；Then it is transferred to again to eurysome golden thread leech supplement screw and produces cocoon pond；
4) verification result
Finally discovery can relatively efficiently improve eurysome golden thread leech Overwintering Survival Percentage to 70% or more, and eurysome golden thread leech exists
It is transferred to after producing cocoon pond, it can be with the effective ovum cocoon of output.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811112002.4A CN109197706A (en) | 2018-09-25 | 2018-09-25 | A kind of eurysome golden thread leech winter low temperature conservation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811112002.4A CN109197706A (en) | 2018-09-25 | 2018-09-25 | A kind of eurysome golden thread leech winter low temperature conservation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109197706A true CN109197706A (en) | 2019-01-15 |
Family
ID=64984970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811112002.4A Pending CN109197706A (en) | 2018-09-25 | 2018-09-25 | A kind of eurysome golden thread leech winter low temperature conservation method |
Country Status (1)
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| CN (1) | CN109197706A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642431A (en) * | 2020-07-03 | 2020-09-11 | 重庆市药物种植研究所 | Method for treating and observing leech living bodies |
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| CN105875508A (en) * | 2014-10-24 | 2016-08-24 | 南宁市德营生物科技有限公司 | New method for soilless overwintering of blood-sucking leeches |
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| CN106614157A (en) * | 2016-09-26 | 2017-05-10 | 南京农业大学 | Indoor artificial leech hibernation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111642431A (en) * | 2020-07-03 | 2020-09-11 | 重庆市药物种植研究所 | Method for treating and observing leech living bodies |
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Application publication date: 20190115 |